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See detailModeling soil respiration in wheat fields
Delogu, Emilie; Le Dantec, Valérie; Mordelet, Patrick et al

Poster (2013, April 09)

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See detailTEMPORAL VARIABILITY OF N2O FLUXES FROM A FERTILIZED GRASSLAND: PRELIMINARY RESULTS FROM DYNAMIC CLOSED CHAMBERS
Beekkerk van Ruth, Jöran ULg; Moureaux, Christine ULg; Degré, Aurore ULg et al

Poster (2013, April 09)

This work presents preliminary results of nitrous oxide (N2O) fluxes measured by dynamic closed chambers from a fertilized grassland grazed by the Belgian Blue breed of cattle. It is part of a project ... [more ▼]

This work presents preliminary results of nitrous oxide (N2O) fluxes measured by dynamic closed chambers from a fertilized grassland grazed by the Belgian Blue breed of cattle. It is part of a project funded by the public service of Wallonia (SPW-DGARNE), whose objectives are to make a carbon/CO2 balance of the grassland (Jérôme et al., 2013) and to quantify CH4 (Dumortier et al., 2013) and N2O fluxes. The site is located in Dorinne (Dorinne Terrestrial Observatory), Belgium (50° 18’ 44” N; 4° 58’ 07” E; 248 m al.). It is a permanent grassland of ca. 4.2 ha with a moderate slope of 1 to 2 %. Mineral fertilisation took place in March and May 2012. Two cylindrical chambers of 19,2 cm diameter and 11,5 cm height were placed inside a protected area around a micrometeorological station. An infrared gas analyser (Thermofischer 46i) was used in order to measure the N2O concentrations inside of the chambers, closed by automatically controlled lids and ventilated by a constant air flow of 1liter/min. These devices were completed by adjacent soil humidity and temperature sensors. The first measurement campaign took place during June and July 2012. The chambers were installed in the field and N2O fluxes were followed without manipulation. N2O fluxes were characterised by a background emission (between 2 and 10 ngN.m2s􀀀1) on which intense but time limited peaks (between 50 and 300 ngN.m2s􀀀1) superimposed. Peaks were found to be mainly linked to fertilisation and driven by precipitation. Background fluxes were found to correlate positively with soil temperature. Secondly, a manipulation experiment took place in November 2012: two different fertilizer treatments were applied to the chambers. Doses of respectively 100 and 200 kg N/ha of ammonium nitrate were sprayed in the chambers (equivalent to a 8mmprecipitation). N2O fluxes peaked shortly after fertiliser application (respectively 300 and 550 ngN.m2s􀀀1), as well as after a posterior rain event (respectively 800 and 1500 ngN.m2s􀀀1). The peak dynamics suggests a complex interaction between soil humidity and nitrogen availability, which is under study. Dumortier et al., Geophysical Research Abstracts, Vol. 15, EGU2013-2083-1, 2013 Jérôme et al., Geophysical Research Abstracts Vol. 15, EGU2013-6989, 2013 [less ▲]

See detailDeciphering human-climate interactions in ombrotrophic peat record : REE, Nd and Pb isotope signatures of dust supplies over the last 2500 years (Misten bog, Belgium)
Fagel, Nathalie ULg; Allan, Mouhamd ULg; le roux, Gael et al

Poster (2013, April 08)

A core of 173 cm of ombrotrophic Misten peat bog from the Hautes-Fagnes Plateau in Eastern Belgium provides a record of Rare Earth Elements (REE) deposition allowing to trace dust fluxes in West Europe ... [more ▼]

A core of 173 cm of ombrotrophic Misten peat bog from the Hautes-Fagnes Plateau in Eastern Belgium provides a record of Rare Earth Elements (REE) deposition allowing to trace dust fluxes in West Europe during the historical record (last 2500 years). REE and lithogenic element analyses, as well as the Nd isotopes, were performed by HR-ICP-MS and MC-ICP-MS, respectively in peat layers dated by 210Pb and 14C. The parallel variations of REE concentration with lithogenic conservative elements confirms that REE are immobile in the studied peat bog and can be used as tracers of dust deposition. Dust fluxes show pronounced increase at BC300, AD600, 1000AD,1200AD and from 1700AD, recording either influence of human activities (regional erosion due to forest clearing and soil cultivation activities) or local and regional climate changes. Using Nd isotope allows to decipher between local and distal causes. The ENd variability (-13 to -9) is interpreted by a mixing between dust sources fromlocal soils and desert particles. Three periods characterised by dominant-distal sources (at 320AD, 1000 AD and 1700AD) are consistent with local wetter intervals as indicated by lower humi[U+FB01]cation degree. Local erosion prevails durier drier (higher humification) intervals (-100AD, 600AD). On a global scale more distal supplies are driven during colder periods, in particular Oort and Maunder minima. Combining geochemical elementary content and isotope data in ombrotrophic peat allows to decipher between dust flux changes related to human and climate forcing. [less ▲]

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See detailAlimentation azotée des animaux de rente
Beckers, Yves ULg

Learning material (2013)

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See detailAmphiphilic structure-surface property relationships of monosaccharide-based surfactants
Razafindralambo, Hary ULg; Nicks, Francois ULg; Richard, Gaetan ULg et al

Poster (2013, April 08)

A set of monosaccharide-based renewable surfactants has been prepared, structurally checked, and systematically screened for different activities and functionalities. These compounds vary in the polar ... [more ▼]

A set of monosaccharide-based renewable surfactants has been prepared, structurally checked, and systematically screened for different activities and functionalities. These compounds vary in the polar head group, linker, and hydrophobic tail. Beyond the goal to find out the most potential valuable surfactants for each measurement, relationships between chemical structure, basic properties, and functionalities have been examined by comparing compounds differing in a single structural variable. In this paper, we show and explain the impact of the anomeric alpha or bêta, neutral or acid sugar derivatives, equatorial or axial position of hydroxyl group, alkyl chain substitute attachment position, number, and length on surface properties of sugar-based surfactants. [less ▲]

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See detailContribution à l'étude des mécanismes transcriptionnels du facteur de transcription SIP1/ZEB2
Koopmansch, Benjamin ULg

Doctoral thesis (2013)

Lors de la conversion métastatique des tumeurs épithéliales, certaines cellules tumorales acquièrent la capacité d’envahir le tissu sous-jacent et de former des métastases à distance. De nombreuses ... [more ▼]

Lors de la conversion métastatique des tumeurs épithéliales, certaines cellules tumorales acquièrent la capacité d’envahir le tissu sous-jacent et de former des métastases à distance. De nombreuses données de la littérature montrent que l’acquisition de ces propriétés est accompagnée d’un phénomène de transdifférenciation appelé « transition épithéliomésenchymateuse » (TEM), impliquant la perte de caractéristiques de cellules épithéliales au profit de caractéristiques de cellules mésenchymateuses. Parmi les modifications moléculaires caractéristiques de la TEM, on observe une diminution de l’expression de cadhérine E ainsi et l’expression de novo de filaments de vimentine. L’expression accrue de différents facteurs de transcription inducteurs de la TEM est aussi rapportée. SIP1 est un des facteurs de transcription impliqués dans les phénomènes de TEM tumorale. Il a été clairement montré que SIP1 réprime l’expression de la cadhérine E en liant son promoteur. Le mécanisme de répression n’est pas précisément connu, mais il n’implique pas le co-répresseur CtBP. Un modèle de répression suggère que SIP1 empêche l’accès de facteurs activateurs aux promoteurs des gènes réprimés. Les données obtenues au cours de ce travail nous permettent d’appuyer cette hypothèse et impliquent KLF4, un facteur de transcription activateur liant le promoteur de la cadhérine E, dans ce modèle. Nos résultats révèlent en effet une compétition entre SIP1 et KLF4 pour la liaison sur le promoteur de la cadhérine E. Nous montrons aussi que les deux facteurs ont des effets opposés sur l’activité du promoteur de la cadhérine E et que KLF4 n’active celui-ci que lorsque SIP1 ne peut s’y lier. Enfin, nos données nous ont permis de localiser plus précisément la région du promoteur de la cadhérine E liée par KLF4. Cette région chevauche un des sites liés par SIP1. SIP1 est considéré comme répresseur de la transcription, mais les données s’accumulent montrant l’induction rapide de gènes cibles de la TEM, dont la vimentine, suite à une surexpression de SIP1. Nous avons entrepris de mieux comprendre l’activation de la transcription par SIP1 en utilisant le promoteur de la vimentine comme modèle, et par une approche globale à l’aide de la technique de ChIPSeq. Cette partie n’a malheureusement pas atteint ses objectifs. [less ▲]

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See detailModélisation du transfert radiatif en forêts feuillues ardennaises : adaptation de la librairie Samsaralight
Ligot, Gauthier ULg; Jonard, Mathieu; de Coligny, François et al

Conference (2013, April 08)

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See detailFrom a source to a sink: the role of biological activities on atmospheric CO2 exchange along the river-ocean continuum
Gypens, N; Passy, P; Lancelot, C et al

Poster (2013, April 07)

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See detailThe influence of biogeochemical processes on the pH dynamics in the seasonally hypoxic saline Lake Grevelingen
Hagens, M; Slomp, C; Meysman, F et al

Poster (2013, April 07)

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See detailVariability of North Sea pH and CO2 pumping in response to North Atlantic Oscillation forcing
Salt, L; Thomas, H; Prowe, F et al

Poster (2013, April 07)

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See detailBranch-and-price: principles and its application to a 2-period vehicle routing problem
Rezaei Sadrabadi, Mahmood ULg

Scientific conference (2013, April 05)

We review branch-and-price as an efficient algorithm to solve integer programming problems with huge number of variables. In particular, we discuss column generation algorithm as the main engine in branch ... [more ▼]

We review branch-and-price as an efficient algorithm to solve integer programming problems with huge number of variables. In particular, we discuss column generation algorithm as the main engine in branch-and-price. Implementation of branch-and-price to solve the most basic version of the well-known vehicle routing problem (VRP) is investigated, and some common tricks are introduced. Then, a new extension of VRP is introduced and exploitation of branch-and-price to solve it is discussed. We consider a 2-period vehicle routing problem where each vertex of the network has a positive demand for period 1, 2, or both. Each demand on period 1 can be postponed to period 2 in order to decrease sum of the routing costs on two periods, but it is penalized in the objective function. Similarly, each demand on period 2 can be advanced to period 1, and yet penalized, with the hope of reducing routing costs. We have used many of the classic tricks to implement branch-and-price for solving our 2-period VRP. We have also used new tricks to (1) possibly improve the upper bound during the course of column generation in each node and (2) decrease the computations time to solve pricing problem in column generation. [less ▲]

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