Last 7 days
Bookmark and Share    
Peer Reviewed
See detailPredicting Grain Protein Content of Winter Wheat
Mansouri, Majdi ULg; Dumont, Benjamin ULg; Destain, Marie-France ULg

in ESANN 2014 Proceedings (2014, April 24)

Detailed reference viewed: 10 (1 ULg)
Full Text
See detailA Frequency-domain Approach to Subspace Identification of Nonlinear Systems, Application to Aerospace Structures
Noël, Jean-Philippe ULg

Doctoral thesis (2014)

The construction of mathematical models from experimental data is an essential step in the design process of engineering systems. The different tasks involved in this activity, from the measurement and ... [more ▼]

The construction of mathematical models from experimental data is an essential step in the design process of engineering systems. The different tasks involved in this activity, from the measurement and processing of data to the validation of the model, fall into the general field of system identification. In structural dynamics, the theoretical and experimental aspects of linear system identification have been successfully addressed since the early seventies, and mature analytical, computational and testing tools have emerged. Nonlinear system identification of vibrating structures has also enjoyed significant advances during the past few years. However, the common practice in industry is to ignore nonlinearities, arguably because their analysis is still regarded as impractical. The objective of this doctoral thesis is precisely to progress towards the development of a practical system identification methodology dedicated to real-life nonlinear structures. The first facet of the thesis is to introduce a nonlinear generalisation in the frequency domain of the so-called subspace identification methods. The proposed frequency-domain nonlinear subspace identification (FNSI) approach yields accurate models of large-scale systems comprising strong nonlinearities, closely-spaced modes and high damping. Because it can also estimate a large number of parameters while maintaining an acceptable computational burden, the second facet of this research is to investigate the utilisation of cubic splines as a very flexible means to model complex nonlinearities. Finally, the third facet of the present work is to derive nonlinear models with optimal statistical properties in the presence of measurement noise. This is achieved by embedding the FNSI method into the maximum likelihood identification framework. The scope of the identification and modelling tools developed in this thesis encompasses nonlinear structural systems originating from the various areas of vibration engineering, including the aerospace, mechanical or civil fields, amongst others. Throughout the dissertation, these tools are illustrated using numerical and experimental structures of increasing complexity, mainly related to aerospace applications. [less ▲]

Detailed reference viewed: 36 (13 ULg)
Full Text
Peer Reviewed
See detailMachine learning techniques to assess the performance of a gait analysis system
Pierard, Sébastien ULg; Phan-Ba, Rémy; Van Droogenbroeck, Marc ULg

Poster (2014, April 24)

This paper presents a methodology based on machine learning techniques to assess the performance of a system measuring the trajectories of the lower limbs extremities for the follow-up of patients with ... [more ▼]

This paper presents a methodology based on machine learning techniques to assess the performance of a system measuring the trajectories of the lower limbs extremities for the follow-up of patients with multiple sclerosis. We show how we have established, with the help of machine learning, four important properties about this system: (1) an automated analysis of gait characteristics provides an improved analysis with respect to that of a human expert, (2) after learning, the gait characteristics provided by this system are valuable compared to measures taken by stopwatches, as used in the standardized tests, (3) the motion of the lower limbs extremities contains a lot of useful information about the gait, even if it is only a small part of the body motion, (4) a measurement system combined with a machine learning tool is sensitive to intra-subject modifications of the walking pattern. [less ▲]

Detailed reference viewed: 22 (2 ULg)
Peer Reviewed
See detailLe psychopathe : apprésentation, chosification de l’alter ego et compétences émotionnelles
Englebert, Jérôme ULg

Conference (2014, April 23)

La psychopathie est une entité diagnostique « trouble », tant au sein de la tradition psychopathologique que dans les nosographies internationales contemporaines (DSM-IV et DSM-V). Le modèle faisant ... [more ▼]

La psychopathie est une entité diagnostique « trouble », tant au sein de la tradition psychopathologique que dans les nosographies internationales contemporaines (DSM-IV et DSM-V). Le modèle faisant autorité en psychologie forensic est celui développé par R.D. Hare (2003) à travers une échelle diagnostique appelée PCL-R. Ce modèle rencontre, d’un point de vue théorique, plusieurs apories, et, d’un point de vue clinique, de nombreuses impasses. Nous proposons de réaliser un exercice de compréhension psychopathologique axé sur notre pratique clinique en nous inspirant des recommandations du courant de la psychopathologie phénoménologique (Minkowski, 1966 ; Binswanger, 1960 ; Stanghellini, 2006 ; Sass, Parnas & Zahavi, 2011). Celui-ci suggère de procéder par la mise en évidence de la structure psychopathologique qui organise le fonctionnement psychologique du sujet. De façon plus précise, nous centrerons notre propos sur deux dimensions essentielles à l’être-au-monde psychopathique. D’une part, nous réaliserons une comparaison avec l’être-au-monde maniaque à travers les variables de l’apprésentation et de la chosification de l’alter ego – telles que les développe Binswanger (1960). Nous discuterons, d’autre part, du vécu émotionnel propre à la psychopathie qui est davantage source d’adaptation (du moins en certaines situations) que de carence véritable. Ce constat nous permettra de rediscuter brièvement de la notion d’empathie en psychopathologie. Nous verrons que ces dimensions propres à l’existence psychopathique font apparaitre une thématique explicitement absente de la nosographie de Hare, celle de la morale. [less ▲]

Detailed reference viewed: 31 (1 ULg)
Full Text
See detailIncertitudes liées à la modélisation agro-environnementale en vue de développer des outils d'aide à la décision
Dumont, Benjamin ULg

Doctoral thesis (2014)

The European Nitrates Directive 91/6/76/EEC aims to ensure water quality by preventing pollution of surface and groundwater induced by nitrates originating from agricultural sources and by promoting ... [more ▼]

The European Nitrates Directive 91/6/76/EEC aims to ensure water quality by preventing pollution of surface and groundwater induced by nitrates originating from agricultural sources and by promoting agronomical good practices. While the implementation of this Directive seems effective, it appears however that the use of nitrogen has still increased by 6% over the last four years in 27 European countries. Furthermore, agricultural sources would be still at the origin of 50% of the total amount of nitrogen discharged into surface waters (http://ec.europa.eu/environment/water/water-nitrates/index_en.html). In Wallonia (Belgium), the Nitrates Directive has been transposed under the Sustainable Nitrogen Management in Agriculture Program (PGDA). Launched in 2002, it involves different sets of actions, like rules definitions concerning fertilizers application, specific and appropriate crop management in vulnerable areas, the control of potentially leachable nitrogen (APL) levels in soils, etc. This is the global context in which lies the present thesis. The main aim is to optimise the nitrogen fertiliser practices to ensure that the needs of a winter wheat culture (Triticum aestivum L.) could be met while reducing the environmental pressure. It relies on the use of crop models, which describe the growth and the development of a culture interacting with its environment, namely the soil and the atmosphere. The major difficulty while working with crop models and model-based decision support tools lies in the fact that different sources of uncertainties have an impact on the modelled phenomena. Indeed, crop models are constituted by a consequent number of differential non-linear equations, involving a lot of parameters which need to be determined as accurately as possible in order to match as close as possible observed sequences of measurements. The first source of uncertainty is thus constituted by the parameters definition. Once the model has been correctly and robustly calibrated it can be used to perform predictions. However, in an agronomical context, the time-delay between sowing and harvest is consequent. As the end-season yield is often the expected output, the uncertainty linked to the non-knowledge of the future implies for the modeller to refer to different hypothesis concerning upcoming climatic scenarios. Finally, moving from models to decision systems dealing with N management involves a last source of uncertainty. Indeed the main problem is that the impact of a given practice is delayed in time from its realisation. In addition to the uncertainty linked to climatic projections themselves, it is highly important to consider the interactions between the practices and the climate. Furthermore, in a decision-making process, it could be highly relevant to know the uncertainty's estimation that could be tolerated on the decision.. Therefore, the present thesis aims to study these different sources of uncertainty in order to design an efficient decision support system. It is divided into five parts. In the first part, a Bayesian sampling algorithm, known as DREAM (DiffeRential Evolution Adaptative Metropolis) will be presented. It was successfully coupled with the STICS soil-crop model used in this study. The a posteriori probability density function of many parameters was sampled in order to improve the simulations of the growth of a winter wheat culture (Triticum aestivum L.). The DREAM algorithm offers different advantages in comparison to usual methods. Among these, it is possible to study i) the most probable a posteriori parameters distributions, ii) the parameters correlations, and iii) the uncertainties impacted on model outputs. Furthermore, a new version of the likelihood function was proposed, making an explicit use of the coefficient of variation. Results showed that it allowed the noise existing on measurements to be considered, but also the heteroscedasticity phenomenon usually encountered in biological growth processes. In parallel, assimilation data is another way to improve models simulations. These techniques allow considering measurements performed in real-time (e.g. remote measures of LAI or soil water content) in order to correct and adjust the possible drift of model simulations. In particular, a recently developed algorithm, known as variational filter, was evaluated. Its superiority, both in term of state variables simulations improvement and parameter resampling, was demonstrated. The third part of the research focuses on the real-time end season yield prediction. It involves building climate matrix ensembles, combining different time ranges of projected mean data and real measured weather originating from the historical records. As the crop growing season progresses, the effects of real monitored data plays a greater role and the prediction reliability increases. Our results demonstrated that a reliable predictive delay of 3-4 weeks before harvest could be obtained. Finally, using real-time data acquired with a micrometeorological station enabled to (i) predict, daily, potential yield at the local level, (ii) detect stress occurrence, and (iii) quantify yield losses (or gains). Being based on projected seasonal norms, this methodology is in opposition to another technique that consists to offer a panel of solution for what concerns the future. Such probabilistic technique relies on the use of stochastic weather generator (LARS-WG in this case). However, in the fourth part of this thesis, on the basis of the convergence in law theorem, it was demonstrated that in 90% of the climatic situations, both approaches were equivalent, exhibiting RRMSE and normalised deviation criteria inferior to 10%. Furthermore the two approaches offered similar predictive delay-time. The main difference between techniques lies in the finality. The first allows to quickly simulate the remaining yield potential, while the second aims to quantify the uncertainty level associated to the predictions. In the fifth and last part of this thesis, in order to quantify the uncertainty level associated to different modalities of N applications, the STICS model answers were studied under stochastic climatic realisations. It was demonstrated that, if no N was applied, under our temperate climatic conditions, the yield distribution could be considered as normal. However, with increasing N practices, the asymmetry level was found itself increasing. As soon as N was applied, not only were the yields higher, but also was the probability to achieve yields that were at least superior to the mean of the distribution. This undoubtedly reduced the risk for the farmer to achieve low yields levels. To summary all the researches conducted in this thesis, a N strategic decision support system was developed. In a general way, for what concerns the Hesbaye Region, the superiority of three fractions N protocols was demonstrated. In addition, the three rates fertilisation management based on the systematic applications of 60 kgN.ha-1 at tillering and stem extension stages and offering the possibility to adapt the flag-leaf fraction in real-time appeared as an optimal strategy. Within this tool, the uncertainty associated to climatic variability could be finely characterised, and the risk encountered by the farmer was quantified for different investigated practices. But far more important, it was demonstrated that N management could be optimised in real-time. In a general way, the research should be pursued by studying more fundamentally and systematically a wide range of different agro-environmental situations. In particular, it would be interesting to study of the Genotype × Environment × Cultural practices interactions to ensure food security in a climatic changing world. [less ▲]

Detailed reference viewed: 63 (26 ULg)
Full Text
Peer Reviewed
See detailPreparation of polyaniline-modified local clay and study of its sorption capacity
Benhebal, Hadj; Chaid, Messaoud; Léonard, Angélique ULg et al

in Journal of Nanostructure in Chemistry (2014), 4(98), 6

Clay minerals are frequently used in adsorption processes with aqueous solution; it was found that the adsorption properties of clays change when the samples are modified. In this context, polyaniline ... [more ▼]

Clay minerals are frequently used in adsorption processes with aqueous solution; it was found that the adsorption properties of clays change when the samples are modified. In this context, polyaniline-modified clay nanocomposite (at 10 %) was prepared by in situ polymerization processes. The structural and morphological characteristics of the synthesized material are systematically examined by X-ray diffraction, scanning electron microscopy, thermo-gravimetry, differential scanning calorimetry and infrared spectroscopy techniques. The adsorption property of the modified clay was evaluated for the removal of a reactive dye (methylene blue) from aqueous solution at room temperature (25 C) via batch adsorption. [less ▲]

Detailed reference viewed: 12 (2 ULg)
Full Text
Peer Reviewed
See detailApplication of isotopic tracers as a tool for understanding hydrodynamic behavior of the highly exploited Diass aquifer system (Senegal)
Madioune, Diakher Hélène; Faye, Serigne; Orban, Philippe ULg et al

in Journal of Hydrology (2014), 511

The Diass horst aquifer system located 50 km east of Dakar (Senegal) is exploited in two main aquifers covered by a sandy superficial aquifer: the confined/unconfined Palaeocene karstic limestone and the ... [more ▼]

The Diass horst aquifer system located 50 km east of Dakar (Senegal) is exploited in two main aquifers covered by a sandy superficial aquifer: the confined/unconfined Palaeocene karstic limestone and the confined Maastrichtian sandstone aquifer underneath. This system has experienced intensive groundwater abstraction during the last 50 years to supply increasing water demand, agricultural and industrial needs. The high abstraction rate from 1989 to 2009 (about 109,000 m3/d) has caused a continuous groundwater level decline (up to 30 m), a modification of the groundwater flow and salinization in parts of the aquifers. The objective of the study is to improve our understanding of the system functioning with regards to high pumping, identify the geochemical reactions that take place in the system, infer origin and timing of recharge by using mainly stable (δ18O, δ2H, 13C) and radioactive (3H and 14C) isotopes. Water types defined in the Piper diagram vary in order of abundance from Ca–HCO3 (65%), Ca/Na–Cl (20%), Na–HCO3 (3%) and Na–Cl (12%). Values of δ18O and δ2H for the superficial aquifer range between −5.8 and −4.2‰ and between −42 and −31‰, respectively. For the Palaeocene aquifer they range from −5.8 to −5.0‰ and from −38 to −31‰, respectively; values in the Maastrichtian aquifer are between −5.9 and −4.3‰ for δ18O and −38 to −26‰ for δ2H. Plotted against the conventional δ18O vs δ2H diagram, data from the upper aquifer exhibit a dispersed distribution with respect to isotopic fractionation while those of the Palaeocene and Maastrichtian aquifers are aligned parallel and slightly below/or on the Global Meteoric Water Line (GMWL) evidencing ancient waters which had evaporated during infiltration. The low tritium (generally <0.7 TU) and 14C (0.7–57.2 pmc) contents indicate predominance of older water being recharged during the Pleistocene and Holocene periods. However, few boreholes which exhibit high tritium (1.2–4.3 TU) and 14C (65.7–70.8 pmc) values indicate some mixture with recent water likely through faulting and vertical drainage from the upper to deeper aquifers as well as lateral flow along flow paths to the piezometric depressions created by pumping. [less ▲]

Detailed reference viewed: 18 (1 ULg)
Full Text
Peer Reviewed
See detailImproving our vision on human cadaveric decomposition chemistry
Stefanuto, Pierre-Hugues ULg; Stadler, S; Perrault, K et al

Conference (2014, April 15)

Detailed reference viewed: 7 (2 ULg)
Full Text
Peer Reviewed
See detailA multiple-level study of metal tolerance in Salix fragilis and Salix aurita clones
Evlard, Aricia ULg; Sergeant, Kjell; Printz, Bruno et al

in Journal of Proteomics (2014), 101C

The response of two willow clones (Salix fragilis (Sf) and S. aurita (Sa)) to the presence of metals (Zn, Cu, Cd, Ni) was studied. Rooted cuttings were planted in control and contaminated soil. After 100 ... [more ▼]

The response of two willow clones (Salix fragilis (Sf) and S. aurita (Sa)) to the presence of metals (Zn, Cu, Cd, Ni) was studied. Rooted cuttings were planted in control and contaminated soil. After 100 days, different parameters (biomass, chlorophyll fluorescence (Fv/Fm), pigment and sugar concentrations, electrolyte leakage and proteome-level changes) were analyzed. The growth of Sa was not influenced by metals whereas Sf produced significantly less biomass when exposed to the pollutants. Furthermore, although Sa did not show a growth reduction in the presence of metals, the overall view of the physiological results among others the changes in the accumulation of sugars and pigments indicated that metals had a more severe impact on this clone. The response at the proteome level confirmed these observations. The growth reduction and the proteomic changes in Sf indicate that this clone adjusts its metabolism to maintain cellular homeostasis. Sa on the contrary maintains growth but the physiological and proteomics data suggests that this can only be done at the cost of cellular deregulation. Therefore high biomass is not linked with a good tolerance strategy. In a long-term study the survival of Sa might be compromised making it a poorer candidate for phytoremediation efforts. [less ▲]

Detailed reference viewed: 10 (0 ULg)
Full Text
Peer Reviewed
See detailThe International Criminal Court as the Principal Authority for Defining the Boundaries of its own Human Rights Obligations
Deprez, Christophe ULg

Conference (2014, April 14)

This paper addresses the applicability of human rights standards to the activity of the International Criminal Court. It argues that, in practice, the Court itself is the key authority when it comes to ... [more ▼]

This paper addresses the applicability of human rights standards to the activity of the International Criminal Court. It argues that, in practice, the Court itself is the key authority when it comes to determining the content of its own human rights obligations. This paradox is due to the fact that the only two legal constructions on which the ICC can convincingly and practically be said to be bound by human rights law – i.e. those that are respectively based on general sources of international law and on the Court’s founding treaty – leave much discretion to the interpreter as to the content of the resulting duties. [less ▲]

Detailed reference viewed: 13 (3 ULg)
See detailDeception and the value of truth: a case study from the fifteenth century
Bauden, Frédéric ULg

Scientific conference (2014, April 13)

Detailed reference viewed: 18 (1 ULg)
Full Text
See detail"Gefühltes Komma - richtiges Komma?"
Münzberg, Franziska ULg

Scientific conference (2014, April 11)

Detailed reference viewed: 11 (0 ULg)
Full Text
Peer Reviewed
See detailPhylogenetic study of Aedes albopictus and Aedes koreicus (Diptera, Culicidae) origin, two invasive mosquito species in Belgium
Raharimalala, Fara Nantenaina ULg; Boukraa, Slimane ULg; Bawin, Thomas ULg et al

Poster (2014, April 10)

The main ways of the introduction of invasive mosquito species in Europe were greatly facilitated by the importation of used tires and plants from the South of China (Dracaena sanderiana, called "Lucky ... [more ▼]

The main ways of the introduction of invasive mosquito species in Europe were greatly facilitated by the importation of used tires and plants from the South of China (Dracaena sanderiana, called "Lucky bamboo"), which are a very good breeding sites of these mosquitoes (www.eidatlantique.eu). For Belgium, several species of exotic mosquitoes have already been listed such as Ochlerotatus japonicus japonicus, Aedes albopictus and A. koreicus. We have captured one male of A. albopictus in Belgium in July 2013 (Boukraa et al, 2013), and teams from other entomologist have been able to find several individuals in the months that have followed our discovery (personal communication). However, his presence was reported first in 2000 (Schaffner et al. 2004) but until 2012, no individuals have been found (Versteirt et al., 2013). We also could find larvae of A. koreicus in abundance. These discovery suggest that these two invasive species are being established in Belgium. Methods: Adult mosquitoes were collected by CO2-baited traps Mosquito Magnet Liberty Plus (MMLP) and immature stages by the dipping method from 8 sites of Belgium. Each individual was then determined morphologically. For A. albopictus and A. koreicus, reconfirmation by molecular method was performed with COI and ND5 mitochondrial primers and sequences were then aligned with those of mosquitoes available in databases by using BioEdit and Multialn softwares. The two gene sequences were concatenated to improve the reliability of the phylogenetic analysis and were carried out by using the Seaview software based on maximum likelihood (ML) methods. Trees were then constructed with the general time reversible (GTR) model, and branch supports were estimated by bootstrapping with 1000 replicates. Result: The aim of this work will focus on the study of the probable origin of these two species of Aedes, which are for the moment safe yet, but that might become dangerous in the event of massive outbreak. The knowledge of the probably origin of the two invasive mosquitoes allow take more protection against their way to enter in the country. [less ▲]

Detailed reference viewed: 9 (0 ULg)
See detailFaire les courses au quotidien. L’approvisionnement alimentaire d’un groupe de migrantes marocaines en Italie en temps de crise
Mescoli, Elsa ULg

Scientific conference (2014, April 10)

La crise généralisée qui, depuis quelques années, a affaibli les économies de nombre d’états, a eu des conséquences sur la typologie des migrations internationales : les trajectoires, les projets, les ... [more ▼]

La crise généralisée qui, depuis quelques années, a affaibli les économies de nombre d’états, a eu des conséquences sur la typologie des migrations internationales : les trajectoires, les projets, les destinations, les départs se sont modifiées en fonction de nouveaux facteurs auxquels les individus et les groupes étaient confrontés (Castles e Vezzoli 2009 ; Arango e González Quiñones 2009 ; Cornelius et al. 2010). A côté de ces macro-dynamiques globales, la crise a également engendré des changements dans la vie quotidienne des migrants lorsqu’ils étaient déjà partis et qu’ils s’étaient déjà installés ailleurs. Dans ce cadre, ma communication vise à analyser les pratiques d’approvisionnement alimentaire d’un groupe de migrantes marocaines en Italie mettant en place des stratégies (Certeau 1980 ; Sassen 2002 ; Rebughini and Sassatelli, 2008; Sassatelli 2009) pour faire face aux conséquences concrètes de la crise économique (notamment la diminution de leur pouvoir d’achat en conséquence de leur précarité professionnelle). Au travers de plusieurs déplacements dans la ville où elles habitaient et de la mobilisation des réseaux familiaux alimentant des circuits informels d’échange de biens, ces femmes satisfaisaient leurs différentes exigences – parmi lesquelles le désir de performer des préparations propres à leur mémoire incorporée – tout en répondant aux contraintes économiques déterminées par la crise. Ma tentative sera de démontrer qu’en dépit des difficultés rencontrées, ces femmes arrivaient à exercer une agency dans le nouvel espace vécu et à se positionner de façon active au travers de leurs choix et conduites quotidiennes. Mon analyse se basera sur les exemples collectés lors de ma recherche doctorale concernant les pratiques culinaires de femmes marocaines en contexte migratoire, et elle sera également supportée par des éléments visuels exprimant la matérialité des dynamiques objets d’étude. [less ▲]

Detailed reference viewed: 18 (1 ULg)
Full Text
See detailEtude du mécanisme d’activation du zymogène proDer p 6 de l’acarien Dermatophagoides pteronyssinus et du rôle de son propeptide dans l’inhibition, le repliement et l’immunogénicité de l’allergène
Herman, Julie ULg

Doctoral thesis (2014)

L’allergie aux acariens touche 20 à 30% de la population occidentale. L’allergène Der p 6 de l’acarien Dermatophagoides pteronyssinus est une protéase à sérine active de la famille de la chymotrypsine ... [more ▼]

L’allergie aux acariens touche 20 à 30% de la population occidentale. L’allergène Der p 6 de l’acarien Dermatophagoides pteronyssinus est une protéase à sérine active de la famille de la chymotrypsine. Son activité protéase pourrait être impliquée dans l’augmentation de la réponse allergique chez les patients atopiques. Der p 6 est synthétisée chez l’acarien sous la forme d’une pré-pro-protéine constituée d’un peptide signal (17 résidus) et d’un zymogène présentant un propeptide de 34 résidus en amont du domaine protéase de 231 résidus. Après avoir produit en Pichia pastoris et purifié le zymogène proDer p 6, son mécanisme d’activation a été élucidé dans la première partie de ce travail. Il s’agit d’un mécanisme tout à fait particulier, propre à l’acarien lors duquel la protéase à cystéine active Der p 1 mature proDer p 6. Un mécanisme similaire avait pu être montré pour le trypsinogène proDer p 3. Pour la première fois, la forme recombinante de Der p 6 a pu être produite. Celle-ci présente la même activité que la protéase naturelle présente dans les extraits d’acariens. Dans la seconde partie de ce travail, Der p 6 ainsi que Der p 1 ont pu être mis en évidence au niveau du tube digestif de l’acarien D. pteronyssinus, ce qui confirme que ces protéases sont impliquées dans la digestion de l’acarien. La protéase Der p 1 est présente au niveau de l’intestin moyen, tandis que Der p 6 est présente dans le gros intestin. Dans cette dernière partie de l’intestin, les protéases sont co-localisées, ce qui indique que le procédé de maturation observé in vitro pourrait se dérouler in vivo. Dans la troisième partie de ce travail, nous avons montré que le propeptide de proDer p 6 est un inhibiteur spécifique de la protéase Der p 6. Le propeptide est donc impliqué dans le contrôle spatio-temporel de l’activation de la protéase. Lors de ce processus, les quatre résidus en amont du site de clivage (résidus P4 à P1) sont nécessaires et suffisants pour la reconnaissance et le clivage du propeptide par Der p 1. Comme cela a été montré pour Der p 3, aucun rôle du propeptide dans le repliement de la protéase Der p 6 n’a pu être mis en évidence. Dans la dernière partie, une étude préliminaire de l’allergénicité de Der p 6 a été réalisée. Der p 6 serait un allergène mineur avec une prévalence d’environ 30 %. Les études par ELISA et activation des basophiles, réalisées avec les formes naturelles, recombinantes ou inactives de Der p 6 indiquent que ces différentes formes pourraient être utilisées en diagnostic. Des prédictions bioinformatiques ont également été réalisées afin de déterminer les épitopes B de Der p 6. [less ▲]

Detailed reference viewed: 9 (5 ULg)