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See detailNovel application assigned to toluquinol: inhibition of lymphangiogenesis by interfering with VEGF-C/VEGFR-3 signaling pathway.
Garcia-Caballero, Melissa; Blacher, Silvia ULg; Paupert, J. et al

in British Journal of Pharmacology (2016), 173(12), 1966-87

BACKGROUND AND PURPOSE: Lymphangiogenesis is an important biological process associated with the pathogenesis of several diseases, including metastatic dissemination, graft rejection, lymphedema and other ... [more ▼]

BACKGROUND AND PURPOSE: Lymphangiogenesis is an important biological process associated with the pathogenesis of several diseases, including metastatic dissemination, graft rejection, lymphedema and other inflammatory disorders. The development of new drugs blocking lymphangiogenesis has become a promising therapeutic strategy. In this study, we aim at investigating the ability of toluquinol, a 2-methyl-hydroquinone isolated from the culture broth of the marine fungus Penicillium sp. HL-85-ALS5-R004, to inhibit lymphangiogenesis in vitro, ex vivo and in vivo. EXPERIMENTAL APPROACH: We used human lymphatic endothelial cells (LEC) to analyze the effect of toluquinol in 2D and 3D in vitro cultures, and in the ex vivo mouse lymphatic ring assay. For in vivo approaches, the transgenic Fli1:eGFPy1 zebrafish, the mouse ear sponges and cornea models were used. Western-blotting and apoptosis analyses were carried out to search for drug targets. KEY RESULTS: Toluquinol inhibited LEC proliferation, migration, tubulogenesis and sprouting of new lymphatic vessels. Furthermore, toluquinol induced LEC apoptosis after 14 h of treatment in vitro, blocked the thoracic duct development in zebrafish, and reduced the VEGF-C-induced lymphatic vessel formation and corneal neovascularization in mice. Mechanistically, we are providing evidence that this drug abrogates the VEGF-C-induced VEGFR-3 phosphorylation in a dose-dependent manner, and represses Akt and ERK1/2 phosphorylations. CONCLUSIONS AND IMPLICATIONS: Based on these findings, we propose toluquinol as a new candidate with pharmacological potential for the treatment of lymphangiogenesis-related pathologies. Notably, its ability to suppress corneal neovascularization paves the way for applications in vascular ocular pathologies. This article is protected by copyright. All rights reserved. [less ▲]

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See detailL’objet d’art et l’expérience du merveilleux mis en mots : le livre de fête comme laboratoire lexical
De Marco, Rosa ULg; Heering, Caroline

Scientific conference (2016, June)

Cette communication entend envisager le vocabulaire artistique tel qu’il s’élabore dans un contexte d’utilisation précis, celui du récit de fête. En marge des écrits théoriques et des principaux réseaux ... [more ▼]

Cette communication entend envisager le vocabulaire artistique tel qu’il s’élabore dans un contexte d’utilisation précis, celui du récit de fête. En marge des écrits théoriques et des principaux réseaux de diffusion d’une lexicographie artistique, le livre de fête se présente en effet comme un précieux laboratoire pour étudier la constitution, l’usage et la circulation d’un vocabulaire artistique dans l’Europe du XVIIe siècle. Destiné à conserver la mémoire de l’événement éphémère, le récit de fête donne aux mots le pouvoir de reproduire l’image de l’objet artistique et de renouveler l’expérience du merveilleux que le lecteur ne peut pas ou plus éprouver. Mis au service de l’ekphrasis, les mots et les procédés rhétoriques y sont chargés de rendre compte des différentes composantes de l’appareil festif (tableau, quadro, pictura, scultura, ornement, etc.), de décrire leurs caractéristiques (forme, couleur, gestuelle, etc.), la manière dont ils sont réalisés (matière, ordonnancement, inventio, technique, etc.) ou la valeur qui y est associée (beauté, nouveauté, prix, ingenium, etc.), mais ont aussi pour tâche de transmettre l’effet produitet recherché sur le public (émerveillement, illusion, plaisir, saisissement,etc.). En envisageant le livre de fête comme un laboratoire lexical à part entière, recouvrant des enjeux qui lui sont propres, il s’agira d’explorer un corpus de textes relatant les festivités jésuites organisées à l’occasion de la canonisation d’Ignace de Loyola et de François Xavier en 1622, lesquelles ont donné lieu à des célébrations dans toute la chrétienté. L’étude synchronique axée sur des comparaisons entre les récits italiens, latins et français permettra de cerner les phénomènes artistiques à travers les mots, de suivre dans le cadre géographique européen les variations expressives des termes et des notions artistiques dans les différentes langues. Un échantillon de mots et de notions inhérents aux champs lexicaux de la description des apparatiet de la perception de l’objet artistique sera confronté aux définitions des dictionnaires, aux traductions et à la littérature artistique contemporains. Une attention particulière sera consacrée aux synthèses théoriques sur le spectaclequi en codifient le langage, comme celles réalisées par Claude-François Ménestrier (1631-1705). [less ▲]

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See detailCompte rendu de Voltaire, Œuvres complètes, vol. 51A : Recueil des facéties parisiennes, éd. dirigée par Nicholas Cronk
Daubercies, Laurence ULg

in COnTEXTES : Revue de Sociologie de la Littérature (2016)

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See detailCartels in the EU: who appeals and who wins?
Gautier, Axel ULg

Conference (2016, June)

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See detailPour une conception deleuzienne de l'interdiscursivité
Franck, Thomas ULg

Conference (2016, June)

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See detailOsteoarthritis biomarkers derived from cartilage extracellular matrix: Current status and future perspectives.
Henrotin, Yves ULg; Sanchez, Christelle ULg; Bay-Jensen, A. C. et al

in Annals of physical and rehabilitation medicine (2016), 59(3), 145-148

Specific soluble biomarkers can be powerful tools for the diagnosis, prognosis and personalized management of osteoarthritis (OA). Biomarkers are potential indicators of the effect of a drug on cartilage ... [more ▼]

Specific soluble biomarkers can be powerful tools for the diagnosis, prognosis and personalized management of osteoarthritis (OA). Biomarkers are potential indicators of the effect of a drug on cartilage metabolism and provide crucial information about the mechanisms of drug action. In this review, we address key questions concerning the use of biomarkers in OA management: Why do we need soluble biomarkers? What are the most widely investigated biomarkers derived from cartilage extracellular matrix? What are the most common pitfalls in interpreting soluble biomarker measurements? What are the perspectives and future research directions in this field? We review current evidence to propose that cartilage-derived soluble biomarkers are complementary "drug development tools" that can be applied during drug development from preclinical research to clinical evaluation. In the future, such biomarkers could be surrogate markers of clinical and/or imaging outcomes. Successful standardization and implementation of automated biomarker assays will facilitate their use in companion diagnostics in the context of personalized medicine for enhanced management of OA. [less ▲]

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See detailEffect of chondroitin sulfate on soluble biomarkers of osteoarthritis: how to analyze and interpret the results from an open-label trial in unilateral knee osteoarthritis patients
Möller, I; Gharbi, Myriam; Martinez, H et al

in Annals of the Rheumatic Diseases (2016, June), 75(Suppl 2), 1167

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See detailStudy of Developmental and Molecular Processes Sorbs1 Regulated by using a combination of in vitro and in vivo models
Bacquelaine Veloso, Alexandra ULg; Dequiedt, Franck ULg; Bruyr, Jonathan ULg et al

Report (2016)

SoHo proteins belong to a family that includes three members:  Sorbs1 (Cbl-associated protein, CAP/ponsin), Sorbs2 (Arg-Binding Protein 2, ArgBP2) and Sorbs3 (Vinexin). These proteins share a similar ... [more ▼]

SoHo proteins belong to a family that includes three members:  Sorbs1 (Cbl-associated protein, CAP/ponsin), Sorbs2 (Arg-Binding Protein 2, ArgBP2) and Sorbs3 (Vinexin). These proteins share a similar structure with a SoHo domain in N-terminal region and three SH3 domains in carboxy-terminal region. These characteristic domains bind to several signaling molecules involved in a variety of cytoskeleton-related processes, and SoHo family members are thus thought to function as adaptor proteins. However, the precise role of these proteins in the cytoskeleton regulation and associated biological functions remains unknown. It is well established that cytoskeleton regulation is critical for various developmental events including angiogenesis, the process by which new blood vessels develop from pre-existing ones. The goal of this project is to identify the developmental function of Sorbs1 and to characterize the underlying molecular events by exploiting a combination of in vivo (Zebrafish) and in vitro (Huvecs) models. In vitro assays showed that Sorbs1 inactivation results in defects of migratory (not shown) and adhesive properties of endothelial cells which leads to problems in tubular formation and sprouting of this last ones, by controlling small Rho-GTPases activity. Sorbs1 morphant zebrafish embryos, revealed abnormal development of venous angiogenic structures (such as caudal vein plexus (CVP) and subintestinal veins (SIV)), which could be related to the defects observed in vitro. These results highlighted important developmental defects following Sorbs1 inactivation in two types of models, which led us to conclude that Sorbs1 could be an important regulator of cytoskeleton-dependent processes, especially during angiogenesis. [less ▲]

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See detailDie Möglichkeiten und Herausforderungen für die Deutschsprachige Gemeinschaft bei der Gestaltung der ihr in der Sechsten Staatsreform übertragenen Zuständigkeiten
Behrendt, Christian ULg

Conference given outside the academic context (2016)

Conférence-débat organisé à Saint-Vith, centre culturel Triangel, 31 mai 2016

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See detailCharacterisation of Phosphate Accumulating Organisms and Techniques for Polyphosphate Detection: A Review
Tarayre, Cédric ULg; Nguyen, Huu-Thanh; Brognaux, Alison ULg et al

in Sensors (2016), 16(797), 1-14

Phosphate minerals have long been used for the production of phosphorus-based chemicals used in many economic sectors. However, these resources are not renewable and the natural phosphate stocks are ... [more ▼]

Phosphate minerals have long been used for the production of phosphorus-based chemicals used in many economic sectors. However, these resources are not renewable and the natural phosphate stocks are decreasing. In this context, the research of new phosphate sources has become necessary. Many types of wastes contain non-negligible phosphate concentrations, such as wastewater. In wastewater treatment plants, phosphorus is eliminated by physicochemical and/or biological techniques. In this latter case, a specific microbiota, phosphate accumulating organisms (PAOs), accumulates phosphate as polyphosphate. This molecule can be considered as an alternative phosphate source, and is directly extracted from wastewater generated by human activities. This review focuses on the techniques which can be applied to enrich and try to isolate these PAOs, and to detect the presence of polyphosphate in microbial cells. [less ▲]

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See detailL’obligation d’aménagement raisonnable pour motif religieux en droit du travail et les ressources du droit du bien-être
Linguelet, Renaud ULg

in Journal des Tribunaux du Travail (2016)

L’obligation d’aménagement raisonnable pour cause de liberté de conscience et de religion peut se définir comme une obligation de nature juridique, applicable dans une situation discriminatoire ou mettant ... [more ▼]

L’obligation d’aménagement raisonnable pour cause de liberté de conscience et de religion peut se définir comme une obligation de nature juridique, applicable dans une situation discriminatoire ou mettant en cause la liberté religieuse d’un individu, qui a pour objectif d’aménager une norme ou une pratique de portée générale. Elle s’exerce dans les limites du raisonnable. Afin de saisir pleinement la portée du concept d’aménagement raisonnable pour motif religieux et ses conséquences en droit du travail, un détour par l’Amérique du Nord s’impose. Par la suite, cet article se focalise sur la possibilité de consacrer l’obligation d’aménagement raisonnable en droit belge. Pour ce faire, les enseignements à tirer du droit européen et communautaire sont mis en lumière, avant de terminer ce tour d’horizon par une analyse des ressources qu’offrent l’article 20, 5°, de la loi du 3 juillet 1978 relative aux contrats de travail ainsi que la législation relative aux risques psychosociaux telle qu’elle a été amendée lors de la réforme de 2014. [less ▲]

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See detailModulation du stress oxydant : nouveaux concepts pour de nouvelles applications
PINCEMAIL, Joël ULg

Scientific conference (2016, May 31)

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See detailI Am Very Sorry Sir! Breaking Bad News to Customers
Delcourt, Cécile ULg; GREMLER, Dwayne; GREER, Dominique

Conference (2016, May 31)

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See detailDie Deutschprachige Gemeinschaft nach der 6. Staatsreform
Bouhon, Frédéric ULg

Conference given outside the academic context (2016)

L'exposé présente les résultats d'une étude sur la Communauté germanophone après la sixième réforme de l'État, menée par l'auteur et deux collègues (étude publiée sous la forme d'un Courrier Hebdomadaire ... [more ▼]

L'exposé présente les résultats d'une étude sur la Communauté germanophone après la sixième réforme de l'État, menée par l'auteur et deux collègues (étude publiée sous la forme d'un Courrier Hebdomadaire du CRISP). Le texte de la conférence offre un résumé de la situation juridique et politique de la Communauté germanophone au milieu de l'année 2016. [less ▲]

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See detailImpact of cord blood banking technologies on clinical outcome: a Eurocord/Cord Blood Committee (CTIWP), European Society for Blood and Marrow Transplantation and NetCord retrospective analysis
Saccardi, Riccardo; Tucunduva, Luciana; Ruggeri, Annalisa et al

in Transfusion (2016), 56(8), 2021-2029

BACKGROUND Techniques for banking cord blood units (CBUs) as source for hematopoietic stem cell transplantation have been developed over the past 20 years, aimed to improve laboratory efficiency without ... [more ▼]

BACKGROUND Techniques for banking cord blood units (CBUs) as source for hematopoietic stem cell transplantation have been developed over the past 20 years, aimed to improve laboratory efficiency without altering the biologic properties of the graft. A large-scale, registry-based assessment of the impact of the banking variables on the clinical outcome is currently missing. STUDY DESIGN AND METHODS A total of 677 single cord blood transplants (CBTs) carried out for acute leukemia in complete remission in centers affiliated with the European Society for Blood and Marrow Transplantation were selected. An extensive set of data concerning CBU banking were collected and correlations with clinical outcome were assessed. Clinical endpoints were transplant-related mortality, engraftment, and graft-versus-host disease (GVHD). RESULTS The median time between collection and CBT was 4.1 years (range, 0.2-16.3 years). Volume reduction (VR) of CBUs before freezing was performed in 59.2% of available reports; in half of these the frozen volume was less than 30 mL. Cumulative incidences of neutrophil engraftment on Day 60, 100-day acute GVHD (II-IV), and 4-year chronic GVHD were 87, 29, and 21 ± 2%. The cumulative incidence of nonrelapse mortality (NRM) at 100 days and 4-year NRM were, respectively, 16 ± 2 and 30 ± 2%. Neither the variables related to banking procedures nor the interval between collection and CBT influenced the clinical outcome. CONCLUSION These findings indicate a satisfactory validation of the techniques associated with CBU VR across the banks. Cell viability assessment varied among the banks, suggesting that efforts to improve the standardization of CBU quality controls are needed. [less ▲]

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See detailStatics and dynamics of magnetocapillary bonds
Lagubeau, Guillaume; Grosjean, Galien ULg; Darras, Alexis ULg et al

in Physical Review. E : Statistical, Nonlinear, and Soft Matter Physics (2016), 93

When ferromagnetic particles are suspended at an interface under magnetic fields, dipole-dipole interactions compete with capillary attraction. This combination of forces has recently given promising ... [more ▼]

When ferromagnetic particles are suspended at an interface under magnetic fields, dipole-dipole interactions compete with capillary attraction. This combination of forces has recently given promising results towards controllable self-assemblies as well as low-Reynolds-number swimming systems. The elementary unit of these assemblies is a pair of particles. Although equilibrium properties of this interaction are well described, the dynamics remain unclear. In this paper, the properties of magnetocapillary bonds are determined by probing them with magnetic perturbations. Two deformation modes are evidenced and discussed. These modes exhibit resonances whose frequencies can be detuned to generate nonreciprocal motion. A model is proposed that can become the basis for elaborate collective behaviors. [less ▲]

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