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See detailInteraction of HTLV-1 Tax with minichromosome maintenance proteins modulates viral transcription
Barez, Pierre-Yves ULg; Carpentier, Alexandre ULg; Boxus, Mathieu et al

Poster (2015, May 13)

First human retrovirus discovered, HTLV-1 infects approximately twenty million individuals worldwide. HTLV-1 is the causative agent of different diseases that include adult T-cell leukemia (ATL) and a ... [more ▼]

First human retrovirus discovered, HTLV-1 infects approximately twenty million individuals worldwide. HTLV-1 is the causative agent of different diseases that include adult T-cell leukemia (ATL) and a neurodegenerative disorder called HAM/TSP (Human associated myelopathy/ Tropical spastic paraparesis). We are interested in the mechanisms of transformation by the viral Tax oncoprotein. We previously showed that Tax interacts with the minichromosome maintenance MCM2-7 helicase and affects host cell replication (Boxus et al, 2012 Blood 119:151). In this project, we focused on the role of the MCM2-7 complex in transcription. We first show by chromatin immunoprecipitation that the MCM2-7 is recruited onto the 5'-LTR promoter. The 5’-LTR does however not act as a DNA replication origin. In contrast, MCM2-7 activates viral transcription as revealed by luciferase reporter assays. Interaction between Tax and MCM2-7 also affect expression of cellular genes. Together, our data thus demonstrate that the viral promoter is not a replication origin and that interaction between Tax and MCM2-7 is involved in the viral transcription. [less ▲]

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See detailThe utility of Google Scholar when searching geographical literature: comparison with three commercial bibliographic databases
Stirbu, Simona ULg; Thirion, Paul ULg; Schmitz, Serge ULg et al

in Journal of Academic Librarianship (2015), 41(3), 322-329

This study aims to highlight what benefits, if any, Google Scholar (GS) has for academic literature searches in the field of geography, compared to three commercial bibliographic databases: Web of Science ... [more ▼]

This study aims to highlight what benefits, if any, Google Scholar (GS) has for academic literature searches in the field of geography, compared to three commercial bibliographic databases: Web of Science (WoS) and FRANCIS (multidisciplinary databases) and GeoRef (specialized in geosciences). To ensure a valid comparison, identical bibliographic searches were applied using each of the four bibliographic tools. To exclude automatic variations of the ten keywords tested, they were placed between quotation marks and searched only in the “title” field of the respective search tools’ interfaces. The results were limited to bibliographic references published from 2005 to 2009. In order to assess the repeatability of the results, the exact same process was repeated monthly between November 2010 and July 2011. Initially the whole set of results was analyzed, after which the search results for two keywords (selected since they yielded a manageable number of results) were studied in more detail. The results indicate that GS search results show a large degree of overlap with those of the other databases but, moreover, yield numerous unique hits, which should be useful to researchers in both the fields of human and physical geography. GS leads the other tools widely on number of results, independently of keyword, subfield, year of publication, or time of search. [less ▲]

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See detailAnticoherence of multiqubit symmetric states
Baguette, Dorian ULg; Bastin, Thierry ULg; Martin, John ULg

Conference (2015, May 13)

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See detailMagneto-optical trapping of iron atoms
Crauwels, Justine ULg; Huet, Nicolas ULg; Krins, Stéphanie ULg et al

Poster (2015, May 13)

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See detailGrowth factor-induced angiogenesis requires uPAR on endothelial cells
Paques, Cécile ULg

Poster (2015, May 13)

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See detailDesign and synthesis of PET-probes targeting AMPA subtype receptors
Deverdenne, François ULg; Claes, Giselle ULg; Goffin, Eric ULg et al

Poster (2015, May 13)

The AMPA subtype of glutamatergic receptors is the main actor in the fast excitatory neurotransmission in the mammalian central nervous system. These receptors are involved in the expression and the ... [more ▼]

The AMPA subtype of glutamatergic receptors is the main actor in the fast excitatory neurotransmission in the mammalian central nervous system. These receptors are involved in the expression and the maintenance of the long-term potentiation, a phenomenon closely linked to cognitive and memorization processes. Based on experimental data, it also appears that glutamatergic systems are involved in several pathological diseases. For instance, a lack of glutamatergic neurotransmission is observed in cognitive disorders or schizophrenia and an excessive activity is observed in Parkinson or Huntington diseases. The in vivo study of glutamate receptors mapping and its evolution appears to be an essential step for a better understanding of its implications. However, according to the literature, design of such a probe remains difficult due to the lack of specificity of the probes. Taking into account the potential in vitro and in vivo activity and specificity of benzothiadizine dioxides (BTDs) acting as AMPA positive allosteric modulators, we are investing the development of new compounds of this class radiolabeled with a fluorine-18 atom. Hence, we are currently developing new series of BTDs characterized by the presence of a fluorine atom and a 7-phenoxy-substituent that are expected to be more active and more specific. Finally, pharmacological tests to evaluate the best candidates for the radiochemical synthesis and in vivo evaluations are currently in progress. [less ▲]

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See detailAn encapsulation-dehydration method for the long-term storage of cyanobacterial strains in the BCCM/ULC collection.
Crahay, Charlotte ULg; Mari, Maude; Wilmotte, Annick ULg

Poster (2015, May 13)

Long-term genetic and functional stability is a fundamental requirement for the maintenance of microorganisms. Cryopreservation is the preferred method for the long-term storage of many micro-organisms ... [more ▼]

Long-term genetic and functional stability is a fundamental requirement for the maintenance of microorganisms. Cryopreservation is the preferred method for the long-term storage of many micro-organisms, including cyanobacteria. The BCCM/ULC collection currently holds over 200 cyanobacterial strains as living cultures, but only 62 are maintained in a cryo-preserved state. The main limiting factors are the low levels of survival of some strains, as well as the long periods required to recover from cryopreservation, and thus the inability to rapidly deliver strains to clients.. The BRAIN-be project PRESPHOTO (preservation of photosynthetic micro-algae in the BCCM collections) (www.presphoto.ulg.ac.be) aims to improve the preservation of cyanobacterial and diatoms in the BCCM/ULC and BCCM/DCG collections, respec-tively. The encapsulation-dehydration is often considered as a promising alternative to the traditional cryopreservation method for recalcitrant microalgal strains. In this technique, cyanobacterial cultures are entrapped in calcium-alginate beads, osmotically dehydrated (traditionally with sucrose solutions), then evaporatively desiccated in a sterile air flow, or over silica gel and subsequently cryopreserved. In this study, the encapsulation-dehydration method was evaluated as potential long-term preservation technique of cyanobacterial strains. The effects of several factors on the viability of 4 strains have been investigated to determine which are the most important for the successful cryopreservation. In particular, several cryoprotectants (i.e. sucrose, PEG or mannitol), methods of beads dehydration (i.e. under a laminar flow or with silica gel) were tested. We have also compared storage at -70°C and in liquid nitrogen (-196oC). A pre-cultivation step in KCl was also tested for its ability to enhance desiccation tolerance. In addition, the use of alginate dissolution buffer following thawing was also considered. In the final phase of the project, the selected cryopreservation protocols will be tested on a large set of strains. In addition, an independent validation of the protocols will be also performed by both partners of the project (BCCM/DCG and the Culture Collection of Algae and Protozoa). [less ▲]

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See detailFlemish nationalism and hip-hop in Belgium Politics v/s music between ethnicity and class
Mazzola, Alessandro ULg

Scientific conference (2015, May 12)

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See detailValorisation de la biomasse par fermentation : biométhanisation et biohydrogène
Hiligsmann, Serge ULg

Scientific conference (2015, May 12)

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See detailMarcello Venusti nel mondo di Perino del Vaga
Geremicca, Antonio ULg

Conference (2015, May 12)

Detailed reference viewed: 16 (3 ULg)
See detailRemote sensing of the atmospheric composition in the infrared spectral region within the Network for the Detection of Atmospheric Composition Change (NDACC) and the Total Carbon Column Observing Network (TCCON)
Notholt, J; Blumenstock, T; Deutscher, N et al

Conference (2015, May 12)

Remote sensing has been established as a powerful tool in atmospheric research. Throughout the last decades satellite and ground-based remote sensing instruments and methods have been developed to sample ... [more ▼]

Remote sensing has been established as a powerful tool in atmospheric research. Throughout the last decades satellite and ground-based remote sensing instruments and methods have been developed to sample the atmosphere from the microwave to the UV/Vis. The international ground based networks NDACC-IR and TCCON are based on solar absorption spectrometry in the infrared. Both networks consist of more than 30 observations sites around the globe, from the high Arctic through mid-latitudes and the tropics to the southern hemisphere and Antarctica. NDACC concentrates on stratospheric observations in relation to ozone chemistry, in many instances, information on the vertical distribution of the target species is determined. Measured trace gases include O3, HCl, HF, HNO3, ClONO2 and many others. In addition, the tropospheric composition is studied by measuring anthropogenic and biogenic species including HCN, OCS, H2O, CO, CH2O, C2H6, and C2H2. The aim of TCCON is to acquire accurate and precise column-averaged abundances of CO2, CH4, N2O, i.e. atmospheric trace gases which have a very small natural variability. TCCON measurements are linked to WMO calibration scales by comparisons with co-incident in situ profiles measured from aircraft or balloon. Results from both networks have been used in many studies in relation to stratospheric ozone chemistry, air-pollution, and with regard to the carbon-cycle. Long-term series are necessary for trend analysis, gaining insight into annual and longer term variability and placing into context shorter term process studies. Due to the similar observation geometry, the ground-based observations are optimally suitable for satellite and model validation and form an essential part of many satellite projects. They also play an important role in the validation of the Copernicus Atmospheric Monitoring Service. In our contribution we will give an overview on the current status of both networks, ongoing efforts to improve network coverage, precision and accuracy, and several examples of scientific highlights. [less ▲]

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See detailCortical reorganization in an astronaut's brain after long-duration spaceflight.
Demertzi, Athina ULg; Van Ombergen, Angelique; Tomilovskaya, Elena et al

in Brain Structure & Function (2015)

To date, hampered physiological function after exposure to microgravity has been primarily attributed to deprived peripheral neuro-sensory systems. For the first time, this study elucidates alterations in ... [more ▼]

To date, hampered physiological function after exposure to microgravity has been primarily attributed to deprived peripheral neuro-sensory systems. For the first time, this study elucidates alterations in human brain function after long-duration spaceflight. More specifically, we found significant differences in resting-state functional connectivity between motor cortex and cerebellum, as well as changes within the default mode network. In addition, the cosmonaut showed changes in the supplementary motor areas during a motor imagery task. These results highlight the underlying neural basis for the observed physiological deconditioning due to spaceflight and are relevant for future interplanetary missions and vestibular patients. [less ▲]

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See detailIntroduction
Cormann, Grégory ULg

Conference (2015, May 11)

Detailed reference viewed: 20 (3 ULg)
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See detailOn the nature of cosmological time
Hauret, Clémentine ULg

Conference (2015, May 11)

Detailed reference viewed: 9 (2 ULg)
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See detailLa problématique des conditionnalités dans les relations de coopération sino-congolaises et euro-congolaises
Baenda Fimbo, Zacharie ULg

Scientific conference (2015, May 11)

Les exigences politiques et économiques qui accompagnent les interventions chinoises et européennes ne cessent de faire l'objet de controverses. Cependant, les conditionnalités européennes plus politiques ... [more ▼]

Les exigences politiques et économiques qui accompagnent les interventions chinoises et européennes ne cessent de faire l'objet de controverses. Cependant, les conditionnalités européennes plus politiques visant le développement des pays partenaires à travers des reformes politiques et institutionnelles sont perçues comme une immixtion dans les affaires intérieures nationales. Alors que les préalables chinois plus économiques visent le développement des pays partenaires par le commerce et les infrastructures. Elles se trouvent aux antipodes de la politique de coopération élaborée par les bailleurs de fond européens. La Chine apparait comme un partenaire attractif et une nouvelle source de financement. Non exemptée des critiques et de zones d'ombre, la coopération chinoise s’intéresse aux infrastructures dont la RDC a besoin pour l'émergence de son économie afin de favoriser ses exportations minières dont la Chine a besoin. [less ▲]

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