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See detailLe droit européen de l’abus de position dominante en 2014
Petit, Nicolas ULg

in Contrats Concurrence Consommation (2015)

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See detailStrategies of regioselective radiolabeling of Nanofitin binder for imaging
Goux, Marine ULg; Dammicco, Sylvestre ULg; Becker, Guillaume ULg et al

Poster (2015, May)

Recently, new strategies emerged in the field of monoclonal antibodies radiolabeling for PET imaging with the use of positron emitter such as fluorine-18, zirconium-89 or gallium-68. Despite their ... [more ▼]

Recently, new strategies emerged in the field of monoclonal antibodies radiolabeling for PET imaging with the use of positron emitter such as fluorine-18, zirconium-89 or gallium-68. Despite their important role in the therapeutic world, antibodies have many disadvantages related to their structure. Moreover, conjugation of chelating agent often occurs on lysines, which is non-regioselective and leads to a heterogeneous mixture of products. In addition, the slow clearance of antibodies can be a problem to obtain a good contrast when they are used in imaging. To address these different limitations, we developed a chemistry-free chelating system consisting of a highly phosphorylatable peptide tag fused genetically to a Nanofitin. A specific phosphorylation step, with the alpha subunit of the casein kinase II, generates a nanocluster of 4 phosphates that can interact strongly with metal ion like zirconium or gallium. This strategy has already demonstrated its powerfulness for the stable and specific anchoring of protein on zirconium phosphonate-based microarray [1]. Considering this tag has been created to specifically anchoring protein on zirconium phosphonate-based microarray, we are currently working on a sequence derived from calcium-binding proteins to chelate specifically lanthanides[2]. As described by Pardoux et al.[3], our strategy is to functionalize this sequence with a phosphate nanocluster able to chelate zirconium or gallium. Our first results shown that a mono-phosphorylatable tag phosphorylated in vitro is able to chelate terbium(III) with a lower affinity than the wild type. Considering that terbium(III) is bigger than gallium(III) or zirconium(IV), we can suppose that the cage obtained is too small but suitable for other ions. In order to validate our hypothesis, we have planned to radiolabel those tags and determine their affinity for gallium(III). In order to validate the use of Nanofitin as a potent alternative tool for in vivo imaging, we have made biokinetic studies in mice with PET and MRI imaging. In these studies, we have radiolabelled with fluorine-18 a Nanofitin and we are currently making use of its specific binding to a cell-surface receptor to target a very precise cell population by using an animal model. Once the phosphorylatable tag optimized for regioselective radiolabelling and the Nanofitin targeting validated in an animal model, the next steps will be to combine these two approaches: we will fuse genetically the tag to the specific Nanofitin, radiolabel it with gallium-68 and perform the biokinetic study of this new radiopharmaceutical product. [1] M. Cinier, M. Petit, F. Pecorari, D. R. Talham, B. Bujoli, and C. Tellier, “Engineering of a phosphorylatable tag for specific protein binding on zirconium phosphonate based microarrays.,” J. Biol. Inorg. Chem., vol. 17, no. 3, pp. 399–407, Mar. 2012. [2] L. J. Martin, “Development of Lanthanide-Binding Tags (LBTs) as powerful and versatile peptides for use in studies of proteins and protein interactions,” 2008. [3] R. Pardoux, S. Sauge-merle, D. Lemaire, P. Delangle, L. Guilloreau, J. Adriano, and C. Berthomieu, “Modulating Uranium Binding Affinity in Engineered Calmodulin EF-Hand Peptides : Effect of Phosphorylation,” PLoS One, vol. 7, no. 8, 2012. [less ▲]

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See detailLa robinsonnade d’anticipation. Sur une forme composite et ses péripéties
Stienon, Valérie ULg

in Nineteenth Century French Studies (2015), vol. 43(n°3-4), 250-266

In nineteenth-century French literature, adventure novels and futuristic fictions share various narrative and thematic features which generate an extensive set of “science fiction adventure” novels that ... [more ▼]

In nineteenth-century French literature, adventure novels and futuristic fictions share various narrative and thematic features which generate an extensive set of “science fiction adventure” novels that have not attracted much critical attention to date. However, such hybrid narratives lead us to reconsider a certain kind of futuristic fiction as it is defined as much by a geographical shift as by time travel. The particular and prolific case of the robinsonnade, regarded as a pivotal subgenre of adventure, facilitates highlighting such generic interferences. This paper examines four novels related either to utopian travel literature or to Jules Verne’s literary and editorial paradigm of the “voyages extraordinaires” in order to shed light on the dynamics framing the earliest forms of science fiction built on the topic of remote, flying, or spatial island. (In French) [less ▲]

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See detailDetermination of Stress and Strain Fields in Cast and Heat Treated Bimetallic Rolling Mill Rolls
Neira Torres, Ingrid; Tchuindjang, Jérôme Tchoufack ULg; Sinnaeve, Mario et al

in Proceedings of the Iron & Steel Technology Conference and Exposition (AISTech 2015 & ICSTI) (2015, May)

Efficient work rolls for Hot Strip mill roughing stands are usually produced as bimetallic spun cast rolls with a core material made of Spheroidal Graphite Iron (SGI) and a shell material made of High ... [more ▼]

Efficient work rolls for Hot Strip mill roughing stands are usually produced as bimetallic spun cast rolls with a core material made of Spheroidal Graphite Iron (SGI) and a shell material made of High Chromium Steel alloy, semi-High speed steel or adapted High Speed steel chemistry. In this paper, the evolution of stress fields and microstructure during post casting cooling and subsequent heat treatment of a standard high chromium steel is targeted. This knowledge is a first step to analyze the different events that could happen during these production stages and provides the residual stress fields as well as the final roll microstructure which are interesting data for roll service life. As far as roughing mill work rolls for roughing mills are concerned, current trends in terms of work roll design are forcing the roll producer to go towards higher usable shell thicknesses or increased residual shell thickness after scrap diameter in order to delay potential fatigue phenomena at the shell-core interface. One important parameter could not be evaluated in this study, it concerns the pollutions of the core material by alloying elements of the shell during production, which could affect to some extent the mechanical properties and microstructure of the core. The macroscopic thermo-mechanical-metallurgical Finite Element model used to perform simulations, takes into account coupled effects. Input data parameters are identified by experimental tests such as compression tests at constant strain rate and numerical procedures such as inverse method. After the model presentation, the material data set is commented. Whole experimental campaign could not be presented, however interested readers are referred to [1] where more details on the material study of High Chromium Steel alloy can be found. The section of simulation results show the phase transformation histories and stress profiles of a typical roll of diameter 1.2 m and shell thickness of 0.08 m. An additional sensitivity analysis of the results to some material data such as induced plasticity transformation coefficient and shift of the martensitic transformation start temperature parameters is reported. Finally, some conclusions analyze the interest and drawbacks of the described model and its results. [less ▲]

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See detailUne histoire de la mobilité à Liège : dates et événements-clés
Frankignoulle, Pierre ULg

in Frankignoulle, Pierre (Ed.) Quand la mobilité fait la ville (et inversement) (2015)

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See detailTaming uncertainty: Towards a new governance approach for nuclear waste management in Belgium
Parotte, Céline ULg; Delvenne, Pierre ULg

in Technology Analysis & Strategic Management (2015)

We focus on the new governance practices in Belgian nuclear waste management from its ‘participatory turn’ in the late 1990s. Rather than praising (or rejecting) participation versus expert analysis, we ... [more ▼]

We focus on the new governance practices in Belgian nuclear waste management from its ‘participatory turn’ in the late 1990s. Rather than praising (or rejecting) participation versus expert analysis, we make use of a theoretical and analytical framework in which the relevant dynamics for the analysis are ‘opening up’ and ‘closing down’ technological appraisals and commitments. Even though nuclear waste management agencies often plead for an integrative approach between expert analysis and stakeholder participation, in practice both exercises are often kept separate. We address this separation and its consequences and we find that societal concerns remain subsumed in the technical options that have long been favoured by the Belgian agency. This article encourages scholars, waste managers and decision-makers to scrutinise the moments and situations in which opening up would be desirable, and when, by contrast, it would be better to close down options in nuclear waste management. [less ▲]

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See detailDo Customers Dare to Share? Exploring Risk Perception and Reduction in Access-Based Services
Hazee, Simon ULg; Delcourt, Cécile ULg; Van Vaerenbergh, Yves

Conference (2015, May)

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See detailEpidemiological aspects and genotypic characterization of strains of Microsporum audouinii isolated in the context of a Belgian National survey on anthropophilic tinea
SACHELI, Rosalie ULg; Dekkers, Charlotte; DARFOUF, Rajae ULg et al

Poster (2015, May)

Objectives The last two years, clinical cases of tinea capitis caused by Microsporum audouinii (M. audouinii), have increased in Belgium. To better understand the emergence of this species in the ... [more ▼]

Objectives The last two years, clinical cases of tinea capitis caused by Microsporum audouinii (M. audouinii), have increased in Belgium. To better understand the emergence of this species in the population, the Belgian National Reference Center (NRC) for dermatophytes launched a national survey in 2013. Epidemiological aspects and genotypic characterization of the strains were included. Methods The study was conducted from March 2013 up to February 2014. All Belgian laboratories were asked to send M. audouinii strains isolated from hair to the NRC with a form to fill in including epidemiological informations. The fungal strains were identified by microscopy or ITS sequencing in case of doubt. The genotypic analysis was performed by the DiversiLab® system (bioMérieux) for DNA fingerprinting and analysis. Epidemiological informations were analyzed with the help of a biostatistician. Results Among the collected isolates, 117 strains have been currently confirmed as M. audouinii. Analysis of the epidemiological characteristics of the infected population shows that the main age category concerns 5-9 year-old children (64%, p< 0,0001) with a sex-ratio M/F of 1.97. Data concerning the geographic origin of the family have been obtained in only 33,6% of the cases. It reveals that strains have been mainly isolated from patients with a Belgian nationality (43,6%) suggesting bias in the data collection. The geographic origin of the remaining group includes several African countries such as Congo (20,61%), Guinea (12,8%) and Burundi (5,12%). The genotypic analysis led to the distinction of 6 genotypic variants of M. audouinii. One of these variants was exclusively recovered from South Belgium (11 strains). The major group was composed of 96 strains, well distributed in different Belgium locations. Two other groups of three strains each were close to the major group but the analysis of the spectral superposition showed some differences between these groups. The two last groups were clearly different from the major group but species identification was confirmed by ITS sequencing. Conclusion The results of the genomic analysis by Diversilab, show that several groups of M. audouinii isolates co-exist in Belgium providing evidence of genetic heterogeneity. However, no clear correlation could be established between the appartenance to a group and epidemiological factors, such as the age or ethnical origin. ________________________________________ [less ▲]

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See detailIn vitro evaluation of fermentation characteristics of two types of insects, as potential novel protein feeds for pigs
POELAERT, Christine ULg; Beckers, Yves ULg; Despret, Xavier et al

in 13th International Symposium on Digestive Physiology of Pigs (2015, May)

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See detailA spectroscopic investigation of the O-type star population in four Cygnus OB associations. II. Determination of the fundamental parameters
Mahy, Laurent ULg; Rauw, Grégor ULg; De Becker, Michaël ULg et al

in Astronomy and Astrophysics (2015), 577

Aims: Having established the binary status of nineteen O-type stars located in four Cygnus OB associations, we now determine their fundamental parameters to constrain their properties and their ... [more ▼]

Aims: Having established the binary status of nineteen O-type stars located in four Cygnus OB associations, we now determine their fundamental parameters to constrain their properties and their evolutionary status. We also investigate their surface nitrogen abundances, which we compare with other results from the literature obtained for galactic O-type stars. Methods: Using optical spectra collected for each object in our sample and some UV data from the archives, we apply the CMFGEN atmosphere code to determine their main properties. For the binary systems, we have disentangled the components to obtain their individual spectra and investigate them as if they were single stars. Results: We find that the distances of several presumably single O-type stars seem poorly constrained because their luminosities are not in agreement with the "standard" luminosities of stars with similar spectral types. The ages of these O-type stars are all less than 7 Myr. Therefore, the ages of these stars agree with those, quoted in the literature, of the four associations, except for Cyg OB8 for which the stars seem older than the association itself. However, we point out that the distance of certain stars is debatable relative to values found in the literature. The N content of these stars put in perspective with N contents of several other galactic O-type stars seems to draw the same five groups as found in the "Hunter" diagram for the O and B-type stars in the LMC even though their locations are obviously different. We determine mass-loss rates for several objects from the Halpha line and UV spectra. Finally, we confirm the "mass discrepancy" especially for O stars with masses smaller than 30 Msun;. [less ▲]

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See detailAnatomic features underlying wood density, in 110 rainforest tree species from central Congo basin
de Haulleville, Thalès ULg; Rousseau, Mélissa; Morin-Rivat, Julie ULg et al

Poster (2015, May)

Investigate the influence of fiber thickness and vessel diameter on the wood density in 110 rainforest tree species, and the relationships between wood density, wood water content and shrinking ratio.

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See detailMolecular analysis of miRNA expression profiles in AIP mutation positive somatotropinomas
Falk, N; Daly, Adrian ULg; Beckers, Albert ULg et al

in Endocrine Abstracts (2015, May)

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See detailThe Clinical characteristics of X-linked acro-gigantism syndrome
Daly, Adrian ULg; Trivellin, G; Rostomyan, Liliya ULg et al

in Endocrine Abstracts (2015, May)

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See detailRecurrence of GH-secreting pituitary adenoms during puberty in children with germline AIP mutations : a clinical challenge
Filliponi, S; Esposito, V; Daly, Adrian ULg et al

in Endocrine Abstracts (2015, May)

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See detailLes réseaux lettrés du diplomate-écrivain Marcel-Henri Jaspar dans les années 1960. De la raison de Renan à l’ésotérisme de De Becker
Genin, Vincent ULg

in Textyles : Revue des Lettres Belges de Langue Française (2015), 46

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