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See detailLes appareils idéologiques du Prince. Une lecture de “Machiavel et nous” (Althusser)
Mancuso, Eva ULg

Scientific conference (2015, April 25)

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See detailEvaluation of a commercially developed semi-automated PCR-surface enhanced raman scattering assay for the detection of Candida species in blood
HAYETTE, Marie-Pierre ULg; WERY, Marie ULg; BOREUX, Raphaël ULg et al

Poster (2015, April 25)

Objectives Microbiological diagnosis of invasive candidiasis is still dependent on culture-based methods. The use of beta-D-glucan antigen detection is included in the EORTC microbiological diagnostic ... [more ▼]

Objectives Microbiological diagnosis of invasive candidiasis is still dependent on culture-based methods. The use of beta-D-glucan antigen detection is included in the EORTC microbiological diagnostic criteria but is rarely available in the clinical labs. On the other hand, PCR-based methods lack standardization. The RenDx Fungiplex® is a new commercially available semi-automated PCR SERS assay designed for the detection of Aspergillus sp. and Candida sp. including the differentiation of resistant strains as C. glabrata, C. krusei and A. terreus. This study was performed for sensitivity and reproducibility testing of the method on 8 different Candida species. Methods The study was conducted on EDTA-blood collected from a healthy donor. Blood samples were spiked with 10 Candida reference strains: C. albicans ATCC 10231, C. albicans NEQAS 1206 and C. albicans NEQAS 2359; C. glabrata ATCC 90030 ; C. krusei ATCC 6258 ; C. tropicalis NEQAS 1036 ; C. guillermondii NEQAS 1035 ; C. parapsilosis ATCC 22019 ; C. lusitaniae NEQAS 1511 and C. dubliniensis IHEM 14280. Spiked samples were diluted at final concentrations ranging from 1 CFU/mL to 1000 CFU/mL. Cultures on Sabouraud dextrose agar were performed in parallel to control yeasts dilutions. DNA extraction was performed by using proteinase K-based method followed by purification on QIAcube automate. The RenDx Fungiplex®kit (Renishaw) was used for the amplification process and the final detection was processed on the SP-1000 sample analyzer. Reproducibility testing was performed on the three C. albicans reference strains by repeating each test 5 times. Results A total of 142 samples were included in the study. A sensitivity of 10 CFU/mL was reached for C. glabrata, C. krusei, C. tropicalis, C. dubliniensis spiked samples while C. lusitaniae and C. tropicalis performed better at 1 CFU/mL. The three tested reference C. albicans strains and C. guillermondii gave the lowest sensitivity (100 CFU/mL). The reproducibility of the assay was 96% Conclusion RenDx Fungiplex®kit allows the detection of the most frequent Candida species responsible for invasive candidiasis in spiked blood samples. The sensitivity of the test is comprised between 10 and 100 CFU/mL for most Candida sp. and reproducibility is very high. This evaluation allows us to consider this commercial kit for inclusion in a clinical study on invasive candidiasis in comparison with non-molecular diagnostic assays. [less ▲]

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See detailTumor suppressive p53 signaling empowers metastatic inhibitor KLF17-dependent transcription to overcome tumorigenesis in non-small cell lung cancer.
Ali, Amjad ULg; Zeeshan Bhatti M; Saboor Shah A et al

in Journal of Biological Chemistry (2015)

Metastasis, which is controlled by concerted action of multiple genes, is a complex process, and is important cause of cancer death. KLF17 is a negative regulator of metastasis and epithelial-mesenchymal ... [more ▼]

Metastasis, which is controlled by concerted action of multiple genes, is a complex process, and is important cause of cancer death. KLF17 is a negative regulator of metastasis and epithelial-mesenchymal-transition (EMT) during cancer progression. However, the underlying molecular mechanism and biological relevance of KLF17 in cancer cells are poorly understood. Here, we show that tumor suppressor protein p53 plays an integral role to induce KLF17 expression in NSCLC. p53 is recruited to KLF17 promoter and results in the formation of p53-DNA complex. p53 enhances binding of p300, and favors histone acetylation on KLF17 promoter. Mechanistically, p53 physically interacts with KLF17 and thereby enhances anti-metastatic function of KLF17. p53 empowers KLF17 mediated EMT genes transcription via enhancing physical association of KLF17 to target gene promoters. Nutlin-3 recruits KLF17 to EMT target gene promoters and results in the formation of KLF17-DNA complex via p53-dependent pathway. p53 depletion abrogates DNA binding affinity of KLF17 to EMT target gene promoters. KLF17 is critical for p53 cellular activities in NSCLC. Importantly, KLF17 enhances p53 transcription to generate a novel positive feedback loop. KLF17 depletion accelerates lungs cancer cells growth in response to chemotherapy. Mechanistically, we found that KLF17 increases tumor suppressor genes p53, p21 and pRB expressions in NSCLC. Functionally, KLF17 required p53 to suppress cancer cell invasion and migration in NSCLC. In sum, our study highlights novel insight into anti-EMT affect of KLF17 via p53-dependent pathway in NSCLC, and KLF17 may be a new therapeutic target in NSCLC with p53 status. [less ▲]

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See detailREGg is critical for skin carcinogenesis by modulating the Wnt/b-catenin pathway
Lei Li; Yongyan Dang; Jishen Zhang et al

in nature communications (2015)

Here we report that mice deficient for the proteasome activator, REGg, exhibit a marked resistance to TPA (12-O-tetradecanoyl-phorbol-13-acetate)-induced keratinocyte proliferation, epidermal hyperplasia ... [more ▼]

Here we report that mice deficient for the proteasome activator, REGg, exhibit a marked resistance to TPA (12-O-tetradecanoyl-phorbol-13-acetate)-induced keratinocyte proliferation, epidermal hyperplasia and onset of papillomas compared with wild-type counterparts. Interestingly, a massive increase of REGg in skin tissues or cells resulting from TPA induces activation of p38 mitogen-activated protein kinase (MAPK/p38). Blocking p38 MAPK activation prevents REGg elevation in HaCaT cells with TPA treatment. AP-1, the downstream effector of MAPK/p38, directly binds to the REGg promoter and activates its transcription in response to TPA stimulation. Furthermore, we find that REGg activates Wnt/b-catenin signalling by degrading GSK-3b in vitro and in cells, increasing levels of CyclinD1 and c-Myc, the downstream targets of b-catenin. Conversely, MAPK/p38 inactivation or REGg deletion prevents the increase of cyclinD1 and c-Myc by TPA. This study demonstrates that REGg acts in skin tumorigenesis mediating MAPK/p38 activation of the Wnt/b-catenin pathway. [less ▲]

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See detailDisrupting the wall accumulation of human sperm cells by artificial corrugation
Guidobaldi, H.A.; Jeyaram, Y.; Condat, C.A. et al

in Biomicrofluidics (2015), 9

Many self-propelled microorganisms are attracted to surfaces. This makes their dynamics in restricted geometries very di erent from that observed in the bulk. Swimming along walls is bene cial for ... [more ▼]

Many self-propelled microorganisms are attracted to surfaces. This makes their dynamics in restricted geometries very di erent from that observed in the bulk. Swimming along walls is bene cial for directing and sorting cells, but may be detrimental if homogeneous populations are desired, such as in counting microchambers. In this work, we characterize the motion of human sperm cells 60 um long, strongly confi ned to 25u m shallow chambers. We investigate the nature of the cell trajectories between the con fining surfaces and their accumulation near the borders. Observed cell trajectories are composed of a succession of quasi-circular and quasi-linear segments. This suggests that the cells follow a path of intermittent trappings near the top and bottom surfaces separated by stretches of quasi-free motion in between the two surfaces, as confi rmed by depth resolved confocal microscopy studies. We show that the introduction of arti cial petal-shaped corrugation in the lateral boundaries removes the tendency of cells to accumulate near the borders, an e ffect which we hypothesize may be valuable for micro fluidic applications in biomedicine. [less ▲]

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See detailLe futur du vieillissement de la population en Wallonie
Delvenne, Pierre ULg

Conference given outside the academic context (2015)

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See detailEffects of enzymatic hydrolysis on conformational and functional properties of chickpea protein isolate
Ghribi, Abir Mokni; Maklouf Gafsi, Ines; Sila, Assaâd et al

in Food Chemistry (2015), 187

The impact of enzymatic hydrolysis by Alcalase on the conformational and functional properties of chickpea protein isolate (CPI) was investigated. The physicochemical, interfacial tension and surface ... [more ▼]

The impact of enzymatic hydrolysis by Alcalase on the conformational and functional properties of chickpea protein isolate (CPI) was investigated. The physicochemical, interfacial tension and surface characteristics of CPI and their hydrolysates (CPH) according to the degree of hydrolysis (DH) were also determined. These parameters were then related to the changes in the emulsification activity (EAI) and stability (ESI). The enzymatic hydrolysis was found to improve protein recovery and solubility, leading to a reduction in the molecular weight bands with a concomitant increase in the intensity and appearance of protein bands having apparent molecular mass below 20 kDa. The interfacial tension decreased from _66.5 mN m_1 for CPI to _59.1 mN m_1 for CPH. A similar trend was observed for the surface charge which declined from _27.55 mV to _16.4 mV for the CPI and CPH, respectively. These changes were found to have a detrimental effect on the EAI and ESI values. [less ▲]

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See detailRetour au travail après un cancer du sein
Mairiaux, Philippe ULg; Schippers, Nathalie ULg; DESREUX, Joëlle ULg et al

Conference (2015, April 23)

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See detailEfficient one-pot synthesis of trans- Pt(II)(salicylaldimine)(4-picoline)Cl complexes: Effective agents for enhanced expression of p53 tumor suppressor genes
Faiz-Ur-Rahman; Ali, Amjad ULg; Rong Guo et al

in Dalton Transactions (2015)

A series of trans-Pt(II)(salicylaldimine)(4-picoline)Cl complexes were synthesized in 78‒87% yield using one-pot procedure from commercially available precursors. The structures of these complexes were ... [more ▼]

A series of trans-Pt(II)(salicylaldimine)(4-picoline)Cl complexes were synthesized in 78‒87% yield using one-pot procedure from commercially available precursors. The structures of these complexes were characterized by 1H, 19F and 13C NMR spectroscopy, HRMS (ESI) as well as single crystal X-ray analysis. Bioactivity investigations including bio-assay, time- and dosedependent, cell cycle progression study, caspase 3 and 9 apoptosis marker assay and DNA interaction using pBR322 plasmid DNA by gel electrophoresis were performed. The results indicated that the complexes showed promising in vitro cytotoxicity in MCF-7 and A549 cancer cell lines. Moreover these complexes enhanced the expression of p53 tumor suppressor gene family members such as p63 and p73. [less ▲]

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See detailLettre à nos amis du Sud
Verjans, Pierre ULg

Article for general public (2015)

Le débat français sur le vote obligatoire peut être alimenté par les expériences belges en la matière. Depuis 1919, la moyenne des absents au vote, des votes blancs et des votes nuls tourne autour de 15 ... [more ▼]

Le débat français sur le vote obligatoire peut être alimenté par les expériences belges en la matière. Depuis 1919, la moyenne des absents au vote, des votes blancs et des votes nuls tourne autour de 15%. On sait que le vote facultatif facilite la mise en place d'un "cens caché". [less ▲]

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See detailOrigines de la "subjectivation": Heidegger, Bachelard
Pieron, Julien ULg

Scientific conference (2015, April 23)

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See detailLes marchés publics et le développement durable
Durviaux, Ann-Lawrence ULg

Conference given outside the academic context (2015)

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See detailMises en prose hagiographiques : questions de méthodologie. Le cas de Barlaam et Josaphat
Lambert, Adelaïde ULg

Diverse speeche and writing (2015)

Séance de séminaire consacrée à quelques outils méthodologiques, théoriques et numériques permettant d'appréhender les particularités que présente le genre hagiographique au sein du corpus des mises en ... [more ▼]

Séance de séminaire consacrée à quelques outils méthodologiques, théoriques et numériques permettant d'appréhender les particularités que présente le genre hagiographique au sein du corpus des mises en prose. [less ▲]

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See detailJustice et Management : les enjeux de la modernisation de la justice
Dupont, Emilie ULg

Scientific conference (2015, April 23)

Cette communication traite des enjeux de la modernisation et de la "managérialisation" de la sphère judiciaire belge. L'exposé s'articule autour d'une présentation de ma recherche doctorale et de mes ... [more ▼]

Cette communication traite des enjeux de la modernisation et de la "managérialisation" de la sphère judiciaire belge. L'exposé s'articule autour d'une présentation de ma recherche doctorale et de mes premiers résultats issus de ma phase exploratoire. [less ▲]

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See detailRessources Métalliques et Economie Circulaire
Pirard, Eric ULg

Conference given outside the academic context (2015)

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See detailEvaluation des systèmes de management de la sécurité et de la qualité de l’aquaculture du tilapia du Nil "Oreochromis niloticus" dans l’Ouest algérien.
Dergal, Nadir Boudjlal ULg

Doctoral thesis (2015)

Cette thèse a été entreprise afin d’élaborer et de développer le premier modèle HACCP spécifique pour la gestion de la qualité ante et post mortem du tilapia (Oreochromis niloticus) produit au niveau de ... [more ▼]

Cette thèse a été entreprise afin d’élaborer et de développer le premier modèle HACCP spécifique pour la gestion de la qualité ante et post mortem du tilapia (Oreochromis niloticus) produit au niveau de la ferme continentale "Fat-Steppes" (Ouest algérien). Pour ce faire, différents points critiques (PCC) ont été déterminés et maitrisés tout au long de la phase d’élevage et de commercialisation. Durant la phase d’élevage, les résidus d’antibiotiques, les résidus de la 17α-méthyle testostérone et la qualité microbiologique et physicochimique de l’eau et de l’aliment d’élevage ont été évalués. Une stratégie analytique pertinente a été développée basée sur une première technique microbiologique de screening qui a été améliorée et partiellement validée pour l’identification des tétracyclines et des fluoroquinolones. Suivie d’une seconde technique LC-UV-MS qui a été spécialement optimisée pour l’identification et la quantification de l’acide oxolinique. Une nouvelle méthode d’extraction, compatible avec un test immuno-enzymatique ELISA (enzyme-linked immunosorbent assay), a été optimisée pour la détection des résidus de 17α-méthyle testostérone (MT) dans la chair du poisson, dans l’eau et l’aliment d’élevage. Le profil nutritionnel du tilapia produit à Fat Steppes a été caractérisé en déterminant sa composition en macronutriments, son profil en acides gras et en acide aminés. Par la suite, l’évolution temporelle de la qualité hygiénique, du tilapia conservé à 4°C et 30°C, a été évaluée par une approche sensorielle, microbiologique (Flore Mésophile Aérobie Totale et Entérobactéries) et biochimique. L’analyse des diènes conjuguées, des hydroperoxydes et des TBARS "Thiobarbituric acid reactive substances" a servi pour évaluer l’oxydation lipidique. L’analyse d’azote basique volatil totat "ABVT", la triméthylamine "TMA" et les amines biogènes a servi pour évaluer la protéolyse bactérienne et enzymatique. Les résultats obtenus ont démontrés des performances satisfaisantes des deux techniques d’analyse des résidus d’antibiotiques. La capacité de détection (CCß) du test de screening est de 0,75 fois la limite maximale de résidus (LMR) de l’acide oxolinique (OXO) et la limite de la quantification chromatographique LC/UV est de LMR/20. Le délai d’attente de l’OXO est estimé à 8 jours après un traitement de six jours successifs avec une dose de 12 mg/ kg de poids vif. La nouvelle méthode d’extraction des résidus d’MT est acceptable en termes de rendement d’extraction (55 % pour la chair et 85 % pour l'eau) et de linéarité (R2 = 0,998). Le délai d’attente des résidus de la MT est estimé à deux mois après un traitement avec une dose ≤ 65 mg MT/kg d’aliment, pendant 28 jours, à partir du 10ème jour post-éclosion. Des seuils microbiologiques de 103 ufc/100 ml de coliformes fécaux et de 3 x 106 ufc/g de flore mésophile aérobie totale (FMAT) sont retenus comme critères de bonne qualité microbiologique de l’eau et de l’aliment d’élevage respectivement. Le tilapia algérien est considéré comme un poisson maigre (± 0,33 % de lipide) et riche en protéines (± 17,3 %). Son profil en acides aminés essentiels et en acides gras polyinsaturés est relativement intéressant. Les paramètres de performance de la nouvelle méthode HPTLC "High Performance Thin Layer Chromatography", développée pour le screening et la quantification de six amines biogènes d’intérêt, sont satisfaisants en termes de linéarité (R2 > 0,98), de justesse (> 84 %), de fidélité. La limite de détection (LOD) de la méthode HPTLC est de l’ordre de 5 µg.g-1 de chair de poisson. Les résultats de l’analyse organoleptique, microbiologique et biochimique sont fortement et positivement corrélés et révèlent que la date d’utilisation optimale du tilapia est limitée à 12 heures et à 5 jours de conservation à 30°C et à 4°C respectivement. Après ces délais, le poisson est rejeté par l’appréciation organoleptique et tous les seuils critiques établis dans ce travail sont dépassés. Des valeurs de l’ordre de : 6 log cfu g-1 pour les FMAT et les entérobactéries, 0,85 mg MDA kg-1 de poids humide, 35 mg ABVT-N 100 g-1 de chair, 8 mg TMA-N 100 g-1 de chair, 100 µg.g-1 de putrescine et de cadavérine sont retenus comme critères microbiologiques et biochimiques respectivement. [less ▲]

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