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See detailLiquidity Constraints and Global Imbalances
Artige, Lionel ULg; Cavenaile, Laurent

Conference (2014, April 05)

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See detailL'ordinaire du contentieux administratif : Analyse du processus décisionnel au Conseil d'Etat belge francophone
Colemans, Julie ULg

Doctoral thesis (2014)

La thèse porte sur le processus décisionnel au Conseil d’Etat belge. Le raisonnement juridique pratique a été étudié à travers les activités concrètes que les auditeurs et conseillers accomplissent tout ... [more ▼]

La thèse porte sur le processus décisionnel au Conseil d’Etat belge. Le raisonnement juridique pratique a été étudié à travers les activités concrètes que les auditeurs et conseillers accomplissent tout au long du parcours du dossier. De cette manière, le raisonnement juridique n’est pas appréhendé comme une opération logique de la pensée mais comme un accomplissement pratique qui se déploient dans un horizon de sens partagé incarné par le concept de « raisonnable ». [less ▲]

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See detailRapports de forces et conflits : « la question balkanique »
Lika, Liridon ULg

Conference (2014, April 04)

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See detailDeconsecrating a doctrinal monument: Raymond M. Lemaire and the revisions of the Venice Charter
Houbart, Claudine ULg

Conference (2014, April 04)

Considering himself as the “main author” of the charter, Raymond M. Lemaire has been one of the first, together with Piero Gazzola, to plead for a revision of the document. As early as 1971, the two men ... [more ▼]

Considering himself as the “main author” of the charter, Raymond M. Lemaire has been one of the first, together with Piero Gazzola, to plead for a revision of the document. As early as 1971, the two men, respectively first Secretary General and President of ICOMOS, tended to launch a debate in favour of a better consideration of the social value of heritage, and the development of specific principles for historical cities conservation, to be included in the Venice charter. Lemaire’s recent experience in that field had indeed convinced him that, contrary to the assertion of article 14, “a literal application of principles valid for monuments, considered as such, is not always possible, nor desirable, for the ensembles”. The adoption of the Amsterdam declaration didn’t put an end to his efforts. Despite his unsuccessful attempt to get a revised version approved by the ICOMOS General assembly in Moscow (1978), R.M.Lemaire always remained critical towards the charter and the application of its principles in the field, underlining, in the eighties, its shortcomings in terms of cultural diversity and, in one of his last texts, in 1996, the negative effect of article 9, leading to the idea that “the mere essence of a conservation operation is a modernist intervention on the edifice or neighbourhood”. Beyond its interest for conservation history, the awareness of Lemaire’s early critical position towards the Venice Charter should inspire the current debates and help us overcome our reluctance to challenge the sacred principles of what we consider a doctrinal monument. [less ▲]

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See detailImaging MS: strategies for the identification of analytes
Debois, Delphine ULg; Smargiasso, Nicolas ULg; Jourdan, Emmanuel et al

Scientific conference (2014, April 04)

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See detailUse of on-line flow cytometry for the characterization of microbial stress dynamics during the bioprocess
Brognaux, Alison ULg; Han, Shanshan; Sorensen, Soren et al

Conference (2014, April 03)

Microbial cell population heterogeneity is now recognized as a major source of issues for the development and optimization of bioprocesses. Flow cytometry is a very powerful tool for the follow up of ... [more ▼]

Microbial cell population heterogeneity is now recognized as a major source of issues for the development and optimization of bioprocesses. Flow cytometry is a very powerful tool for the follow up of physiological properties of microbial cells in process-related conditions at the single cell level, and can be used to study the dynamics of segregation directly in bioreactors. In this context, specific interfaces have been developed in order to connect flow cytometer (FC) directly on bioreactor for automated analyses. In this work, we propose a simplified version of such interface and demonstrated its usefulness for multiplexed experiments. This automated FC system has been tested for the follow up of the dynamics of an E. coli pfis::gfpAAV fluorescent bio-reporter and its PI uptake, correlated with membrane permeability. This bioreporter is composed of a fis promoter, a growth dependent promoter-indicator of the nutrient status of cells, fused to a gene expressing an unstable variant of GFP. The results obtained showed that the dynamics of the GFP synthesis is complex and can be attributed to a complex set of biological parameters. Segregation in the membrane permeability has been noticed. This work demonstrates that a simplified version of on-line FC can be used at the process level for the investigation of the dynamics of complex physiological mechanisms. [less ▲]

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See detailNo simian Plasmodium detected in populations living in the equatorial rainy forest of the Democratic Republic of Congo
Mvumbi makaba, Dieudonné; Bobanga Lengu, Thierry; Kayembe Ntumba, Jean-Marie et al

Poster (2014, April 03)

Background Malaria remains the most deadly parasitic disease to date, especially in sub-Saharan Africa, which comprises the majority of cases collected per year. It has long been accepted that four ... [more ▼]

Background Malaria remains the most deadly parasitic disease to date, especially in sub-Saharan Africa, which comprises the majority of cases collected per year. It has long been accepted that four species of Plasmodium (P. falciparum, P. vivax, P. malariae and P. ovale) were responsible for the disease in humans. But quite recently, a fifth species, Plasmodium Knowlesi, has been identified as naturally infecting humans. Indeed, known for decades as naturally parasitizing the monkey Macaca fascicularis, P. knowlesi has long been confused, in terms of its evolutionary stage, with P. malariae or P. falciparum, which it resembles morphologically and it was not possible to properly differentiate them until the advent of molecular biology. To date, P. Knowlesi has only been identified in Southeast Asia and a similar phenomenon of natural transmission of simian plasmodium to humans has not been reported elsewhere. We therefore conducted this study to investigate the possible transmission of simian plasmodium to humans in populations living near the rainforest of the Democratic Republic of Congo (DRC) where several species of primates lives. Methods & Materials Three villages (Wenji-Secli, Bongonde, and Bolenge) in the Province of Ecuador (North-eastern DRC) were selected because of their geographical location. Blood samples spotted on filter paper were collected from 100 people randomly taken in each village. Two successive RT- PCR were performed. A first one using a single probe able to diagnose all plasmodium spp. and a second using four species-specific probes for the diagnosis of the four conventional human plasmodium species. Positivity in the first RT- PCR with negativity in the second RT- PCR would suggest the presence of plasmodium species other than the four conventional. Results P. falciparum was correctly identified in 44.6 % of samples. No other species of human plasmodium or not has been identified. Conclusion This preliminary study did not detect the presence of simian plasmodium in human populations living in the rainforest of the DRC. Studies with larger samples and with more advanced techniques should still be conducted. Keywords: Malaria, simian plasmodium, DR Congo [less ▲]

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See detailCaractérisation minéralogique, géochimique et géotechnique des matières premières utilisées en poterie dans le site d’Agafay, Marrakech, Maroc.
El Boudour El Idrissi, Hicham; Daoudi, Lahcen; El Ouahabi, Meriam ULg et al

Conference (2014, April 03)

Le site potier d’Agafay est localisé à 20 km au S-W de la ville de Marrakech, à l’ouest de l’oued N’Fis. L’activité potière y a débuté par transfert du savoir-faire à partir du village de Tamsloht vers ... [more ▼]

Le site potier d’Agafay est localisé à 20 km au S-W de la ville de Marrakech, à l’ouest de l’oued N’Fis. L’activité potière y a débuté par transfert du savoir-faire à partir du village de Tamsloht vers 1940 apr. J.-C. Le site comporte environ 90 ateliers où sont actifs 240 artisans. Les principales pièces produites sont les gasriyas (i.e., plats traditionnels de couscous et de modelage de pâtes de pains) et les briques des grandes murailles traditionnelles. Les matières premières utilisées, brutes ou en mélange, sont issues des terres agricoles locales (Ag1 et Ag5) pour les gasriyas et des dépôts actuels du oued N’Fis (Ag7) pour les briques. Les problématiques rencontrées dans ce site concernent les deux types de produits, gasriyas et briques. Dans le premier cas, des grains de la chaux gonflent dans les pièces après le contact prolongé des pièces produites avec l’air. Ce gonflement engendre des décollements dans les tessons et nuit à la qualité du produit final. Dans le deuxième cas, la distribution non homogène de la chaleur à l’intérieur des fours traditionnels utilisés engendre une très grande variation de couleur dans les briques, elles sont généralement tordues et certaines fondent totalement. Notre projet vise à une caractérisation scientifique des différentes phases de fabrication afin de remédier à ces problèmes. Les résultats montrent que la composition chimique est surtout silico-alumineuse (55 à 61 % de SiO2 ; 16 à 20% d’Al2O3), la teneur en Fe2O3 se situe entre 6 et 8 %, celle de CaO ne dépasse pas 6% pour les pâtes des gasriyas et atteint 10 % pour les pâtes des briques. La teneur en carbonates mesurée par le calcimètre de Bernard varie entre 5 et 14 %. La teneur en matière organique varie entre 5 et 7,5 %. L’indice de plasticité oscille entre 16 et 17,5. Pour des teneurs en eau proches de la limite de plasticité le degré de retrait au séchage des gasriyas est de l’ordre de 5 %, il est plus élevé pour les briques (8%) ce qui nécessite une plus grande maitrise du processus de séchage pour éviter que les pièces ne se tordent pas. La granulométrie des pâtes utilisées pour les gasriyas a montré une teneur moyenne de 50 % pour les argiles, 32 % pour les limons et 18 % pour les sables. Les briques comportent une proportion égale des trois classes granulométriques. La minéralogie de l’ensemble des pâtes est formée de quartz (< 27%), plagioclase (13%), calcite (7 à 9%), dolomite (5%) et argile (50%, 34% et 34% pour Ag1, Ag5 et Ag7, respectivement). Le feldspath potassique est présent uniquement dans l’échantillon Ag7. Concernant la fraction argileuse, elle est surtout constituée d’illite pour l’ensemble des pâtes, associée à des interstratifiés irréguliers 14c-14s (13 %) pour la pâte des briques, et une teneur de 11 % de smectite pour la pâte des gasriyas (Ag5). La mesure de la vitesse sonique a montré que les pièces formées par pression aboutissent à une porosité moindre que celle des pièces produites par boudinage. La densité des pièces produites reste semblable pour les cuissons à 950°C et 1050°C. Le plus bas degré de porosité ainsi que les plus grandes valeurs de résistance mécanique à la compression ont été enregistrés sur les pièces cuites à 1050 °C. Les contraintes maximales atteintes se situent entre 51 et 59 MPa pour les gasriyas et 24,5 MPa pour les briques. Quant à la résistance mécanique à la flexion, elle varie entre 17,4 et 20,3 N/mm² pour les gasriyas et 9,4 N/mm² pour les briques. L’analyse DRX des pièces cuites à 1050 °C a montré la disparition totale des carbonates et des argiles à cette température, accompagnée de l’apparition de la géhelénite, cristobalite, anorthoclase, diopside, spinelle et hématite. A l’issue de cette étude, nous recommandons un tamisage humide à 1 mm pour réduire l’effet des gros grains de la chaux, une cuisson par palier jusqu’à 1050°C est également nécessaire pour comprendre les transformations des phases minéralogiques et vitreuses. Finalement, l’utilisation des fours équipés d’une sonde pour garantir une meilleure distribution de la chaleur. [less ▲]

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See detailLa confiance comme processus relationnel. Une tentative de modélisation
Dethier, Baptiste ULg

Scientific conference (2014, April 03)

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See detailNest grouping patterns of bonobos (Pan paniscus) in relation to fruit availability in a forest-savannah mosaic
Serckx, Adeline ULg; Huynen, Marie-Claude ULg; Bastin, Jean-François ULg et al

in PLoS ONE (2014)

A topic of major interest in socio-ecology is the comparison of chimpanzees and bonobos’ grouping patterns. Numerous studies have highlighted the impact of social and environmental factors on the ... [more ▼]

A topic of major interest in socio-ecology is the comparison of chimpanzees and bonobos’ grouping patterns. Numerous studies have highlighted the impact of social and environmental factors on the different evolution in group cohesion seen in these sister species. We are still lacking, however, key information about bonobo social traits across their habitat range, in order to make accurate inter-species comparisons. In this study we investigated bonobo social cohesiveness at nesting sites depending on fruit availability in the forest-savannah mosaic of western Democratic Republic of Congo (DRC), a bonobo habitat which has received little attention from researchers and is characterized by high food resource variation within years. We collected data on two bonobo communities. Nest counts at nesting sites were used as a proxy for night grouping patterns and were analysed with regard to fruit availability. We also modelled bonobo population density at the site in order to investigate yearly variation. We found that one community density varied across the three years of surveys, suggesting that this bonobo community has significant variability in use of its home range. This finding highlights the importance of forest connectivity, a likely prerequisite for the ability of bonobos to adapt their ranging patterns to fruit availability changes. We found no influence of overall fruit availability on bonobo cohesiveness. Only fruit availability at the nesting sites showed a positive influence, indicating that bonobos favour food ‘hot spots’ as sleeping sites. Our findings have confirmed the results obtained from previous studies carried out in the dense tropical forests of DRC. Nevertheless, in order to clarify the impact of environmental variability on bonobo social cohesiveness, we will need to make direct observations of the apes in the forest-savannah mosaic as well as make comparisons across the entirety of the bonobos’ range using systematic methodology. [less ▲]

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See detailApport des examens biologiques en pratique allergologique
GADISSEUR, Romy ULg

Conference (2014, April 02)

Apport des examens biologiques en pratique allergologique

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See detailDiversité et abondance entomologique en cultures maraîchères à Libreville et à Owendo (Gabon)
Bayendi-Loudit, Sandrine ULg; Poligui, René Noël ULg; Ndoutoume Ndong, Auguste et al

Conference (2014, April 02)

In order to improve knowledge of the diversity and abundances of the entomofauna occurring within market gardenings in Gabon, this study was conducted in three localities in Libreville and Owendo, from ... [more ▼]

In order to improve knowledge of the diversity and abundances of the entomofauna occurring within market gardenings in Gabon, this study was conducted in three localities in Libreville and Owendo, from July1st to August 31st, 2012. Insect collections from yellow traps and visual observations were performed weekly within plots in which grow Amaranth (Amaranthus sp., Amaranthaceae), red sorrel (Hibiscus sabdariffa L, Malavaceae), Tomato (Lycopersicon esculentum Mill, Solanaceae) and Cabbage (Brassica oleracea L, Brassicaceae). A total of 7119 insects were assessed and taxonomically classified in 8 distinct orders comprising 81 families. The study highlighted some entomologic families with agronomic interest. The main pests were represented by Aphididae (31,0%), Cecidomyiidae (5,1%), Chrysomelidae (4,0%) and Thripidae (2,0%), the useful insects (pollinators and predators) by Dolichopodidae (5,4%), Andrenidae (2,6%) Coccinellidae (1,7%), Syrphidae (0,6%), Braconidae (0,5%) and Hemerobiidae (0,1%) and the associated insects were Psychodidae (7,4%), Muscidae (5,9%), Formicidae (4,4%) and Anthomyiidae (4,2%). Entomological diversity is discussed according to the local conditions and potential for sustainable pest control encountered. [less ▲]

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See detailA pheromone-based formulation against phytophagous pests
Fassotte, Bérénice ULg; Vandereycken, Axel ULg; Heuskin, Stéphanie ULg et al

Poster (2014, April 02)

Innovative integrated pest management methods are needed to overcome market withdrawal of synthetic pesticides. Therefore, the identification of environment-friendly bio-products carrying direct or ... [more ▼]

Innovative integrated pest management methods are needed to overcome market withdrawal of synthetic pesticides. Therefore, the identification of environment-friendly bio-products carrying direct or indirect biocide activity is one promising alternative option. Our researches focus on the identification of appropriate formulations releasing volatile organic compounds that are attractive for natural enemies of insect pests. However, the elaboration of slow-release devices that ensure stable and controlled release of active volatile compounds is quite challenging. Here, we developed a formulation based on E-β-farnesene and (–)-β-caryophyllene, these two semiochemicals having strong attractive potential on aphid natural enemies including ladybeetles and hoverflies. Both compounds were encapsulated together in alginate gel beads. The blend efficiency is currently being evaluated through laboratory and field assays. [less ▲]

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See detailFirst evidence of a volatile sex pheromone in the invasive Harmonia axyridis (Pallas) (Coleoptera: Coccinellidae)
Fassotte, Bérénice ULg; Fischer, Christophe ULg; Durieux, Delphine ULg et al

Conference (2014, April 02)

Data about sex pheromones, or any semiochemicals that play a role in Coccinellid mating, remain limited. Since years, various studies and behavioral observations have hypothesized that such molecules are ... [more ▼]

Data about sex pheromones, or any semiochemicals that play a role in Coccinellid mating, remain limited. Since years, various studies and behavioral observations have hypothesized that such molecules are involved in sexual communication of ladybeetles. In this study, we collected volatile organic compounds released by virgin females of the multicolored Asian ladybeetle, Harmonia axyridis (Pallas), which were either allowed or not allowed to feed on aphids. In the presence of aphids, virgin females exhibited “calling behavior”, which has been associated with the emission of a sex pheromone in several Coleoptera species. Bioassays showed that these females released a blend of volatile compounds that is involved in the attraction of males. Gas Chromatography-Mass Spectrometry analyses highlighted specific volatile cues emanating from females, whereas males did not produce these compounds. Five components were identified: (–)-β-caryophyllene, β elemene, methyl-eugenol, α humulene, and α bulnesene. All compounds were produced after virgin females were fed aphids, and their quantity increased across the experimental period. The results confirm that female H. axyridis produce a volatile sex pheromone. Therefore, this study provides important biological information that could promote the development of efficient pest control management methods to manipulate the movements of this invasive ladybeetle, and to reduce its negative impacts on biodiversity. [less ▲]

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See detailModélisation 2D par éléments finis du contact : effet de l’utilisation de méthodes de représentation des surfaces présentant un ordre de continuité élevé et méthodes permettant de passer le patch test
Nguyen, Duc Tue ULg

Doctoral thesis (2014)

Ce travail de thèse concerne la mécanique numérique du contact entre les solides déformables. La simulation numérique des problèmes de contact comporte de nombreuses difficultés notamment dans un contexte ... [more ▼]

Ce travail de thèse concerne la mécanique numérique du contact entre les solides déformables. La simulation numérique des problèmes de contact comporte de nombreuses difficultés notamment dans un contexte de grands déplacements et de grandes déformations. Le but de ce travail est d’analyser l’influence de l’ordre de continuité élevé de la représentation de surface et de développer une méthode pour passer le patch test, à savoir qu’un élément passe le patch test si il transmet correctement des pressions de contact entre deux surfaces. Pour la discrétisation des éléments finis, l'interface de contact est représentée par une série de lignes et de courbes à continuité C0. Par conséquent, lors du passage du nœud esclave entre deux segments maîtres adjacents, il peut arriver avoir une discontinuité de la direction du vecteur normal. Cette situation peut mener à des problèmes de convergence de l'algorithme de résolution. Pour éviter ce problème, l’approche proposée consiste à développer les représentations de surface maître à ordre de continuité C1 ou C2 pour les éléments nœud-surface linéaires et quadratiques. Les éléments nœud-surface ne passent pas le patch test. Plus récemment, un élément linéaire utilisant la méthode des nœuds esclaves virtuels pour Passer le Patch Test (PPT) a été proposée dans la littérature. Cependant, cet élément est à ordre de continuité C0 et peut donner des grandes oscillations de la pression de contact à cause des pénétrations initiales des nœuds esclaves virtuels qui sont les pénétrations des nœuds esclaves virtuels dans la surface maître à l’état initial. Pour tenter de surmonter cette difficulté, une nouvelle approche repose sur un élément linéaire utilisant la méthode PPT et la représentation de surface à ordre de continuité C1 pour la surface maître et pour la surface esclave. L’élément quadratique nœud-surface donne des oscillations de pression de contact à cause de la distribution non-uniforme des forces nodales. Afin de résoudre ce problème, la méthode PPT est développée pour l’élément quadratique. [less ▲]

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See detailUtilisation de biocapteurs microbiens GFP pour la caractérisation des performances des bioréacteurs
Brognaux, Alison ULg

Doctoral thesis (2014)

The scale-up of bioprocesses is a critical step during bioproducts development. Actually, the mixing operation’s efficiency drops when the reactor volume increases: gradients of glucose and oxygen appear ... [more ▼]

The scale-up of bioprocesses is a critical step during bioproducts development. Actually, the mixing operation’s efficiency drops when the reactor volume increases: gradients of glucose and oxygen appear when operating in fed-batch mode, causing losses of production. The aim of this project is the scale-up and sizing of bioreactors based on the direct physiological parameters to consider this heterogeneity. Concretely, it consists in obtaining an on-line signal of the physiological status of micro-organisms. The coding sequence of the Green Fluorescent Protein (GFP) has been inserted after gene promoters of interest in Escherichia coli to built biosensors. A particular focus has been paid on promoters responding to general stress or specifically to lack of glucose and on those responding to cell growth rate. The GFP biosensors of interest have been tested in scale-down bioreactors, allowing to reproduce industrial hydrodynamic conditions at a laboratory scale. A mini-bioreactor platform has also been developed to enable a high throughput screening of biosensors. The intracellular accumulation of GFP has been measured by flow cytometry and GFP release has been monitored by western blot analyses. For biosensors sensitive to stress general response or glucose limitation, GFP has been induced during a glucose limitation and repressed by comparison when glucose heterogeneities appear. The use of a destabilized GFP has been considered in this project for ribosomal biosensors to approach more instantaneous physiological responses of microorganisms. For these ones, the response is proportional to growth rate during the batch phase, but more complex mechanisms take part during a prolonged glucose limitation. Membrane permeability has also been studied and has been noticed more important in homogeneous fed-batch bioreactors than in scale-down reactors. As GFP leakage has been noted in the extracellular medium, a study has also been carry out about proteins released in the extracellular medium (leakage), and correlated with the cell permeability. Finally, an on-line flow cytometer has been developed for the characterization of physiological status of micro-organisms during the bioprocess, and a 3D-ORM probe allowed to measure their viability on-line [less ▲]

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See detailL’antonymie. Définition de l’antonymie en langue et description des fonctions sémantico-référentielles de la co-présence antonymique en discours
Steffens, Marie ULg

Doctoral thesis (2014)

Grâce à un examen critique des travaux consacrés à l’antonymie, nous avons pu définir l’antonymie comme une relation multiforme qui touche des unités très différentes sur les plans sémantique et morpho ... [more ▼]

Grâce à un examen critique des travaux consacrés à l’antonymie, nous avons pu définir l’antonymie comme une relation multiforme qui touche des unités très différentes sur les plans sémantique et morpho-syntaxique mais dont l’unicité réside dans une opposition binaire fondamentale enracinée lexicalement. Nous avons ainsi dépassé la simple distinction entre contraires et contradictoires pour entrer dans la fascinante diversité de l’opposition et montrer comme les notions de graduation, de graduabilité, de scalarité, de borne et de seuil se combinent pour forger l’identité sémantique de chaque paire d’antonymes, la rapprocher ou la distinguer des autres. Grâce à l’analyse empirique d’énoncés extraits du journal Le Monde, nous avons montré que la co-présence antonymique en contexte s’appuie pour faire sens sur les relations antonymiques inscrites dans la mémoire linguistique des locuteurs, où sont fortement associées la forme et le sens des antonymes. Nous avons également montré comment la plongée de deux antonymes dans un contexte d’emploi peut moduler leur opposition et la définition du lexème neutre ou intermédiaire, lorsqu’il existe. Les antonymes co-présents, grâce aux rôles sémantico-syntaxiques qu’ils peuvent jouer, contribuent à la structuration des énoncés dans lesquels ils sont employés parce qu’ils organisent les unités qui les entourent sur le plan sémantico-référentiel. [less ▲]

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