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See detailDetection of nitric oxide by diaminofluorescein visualizes the skeleton in living zebrafish.
Renn, Jörg ULg; Pruvot, Benoist; Muller, Marc ULg

in Journal of Applied Ichthyology (2014), 30(4), 701706

Several in vivo stainings, such as Calcein, Alizarin Red and Quercetin are commonly used to visualize ossification in living teleost specimen. These staining techniques represent important tools for bone ... [more ▼]

Several in vivo stainings, such as Calcein, Alizarin Red and Quercetin are commonly used to visualize ossification in living teleost specimen. These staining techniques represent important tools for bone research in fish, but do not visualize cartilage. In the present study, we show that nitric oxide (NO) labelling by DAF-FM DA visualizes both bone and cartilage in vivo during zebrafish skeletogenesis. NO detection performed in Tg(osterix:mCherry) or in combination with Alizarin Red in wild-type zebrafish reveals that intense staining through NO labelling colocalizes with the appearance of osteoblasts and characterizes ossified structures. Cartilage structures are clearly distinguished in the living larvae, although the labelling is less intensive when compared to ossified structures. This method is the first and easy to handle alternative to cartilage and bone double stainings on fixed samples. In contrast to most live skeletal stainings, which only stain the mineralized bone structures, this protocol in addition allows in vivo visualization of cartilage. [less ▲]

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See detailMultidisciplinary approaches and fractionations to study lower termite symbiotic system and ligno-cellulose digestion
Bauwens, Julien ULg; Brasseur, Catherine ULg; Tarayre, Cédric ULg et al

Poster (2014, August)

Wood-feeding termites are a considerable source of enzymes active on ligno-cellulosic compounds. These enzymes are produced by the termite host and some representatives of its symbiotic microbial ... [more ▼]

Wood-feeding termites are a considerable source of enzymes active on ligno-cellulosic compounds. These enzymes are produced by the termite host and some representatives of its symbiotic microbial community, and are of particular interest in regard second generation biofuel. However, the complexity of microbial interactions renders micro-organisms isolation very difficult. Culture-independent methods permitted to gather a large amount of data and to understand a little more the role of each microbial population, particularly the prokaryotes. Proteomics allows working on the final product of gene expression, and corresponds more to the real operation of the digestive system. In order to investigate such a complex system, it is necessary to use multidisciplinary approaches and to fractionate this system. Zymography or affinity chromatography are used in parallel of routine proteomics techniques such as two-dimensional gel electrophoresis associated to MALDI-TOF mass spectrometry and nano-LC ESI-MS/MS. We used an artificial-diet based rearing to induce changes in microbial population balance. We performed preliminary assay to investigate the glycosylated proteome in the hindgut of a lower termite, using Multi-Lectin Affinity Chromatography (M-LAC) and enzymatic activity of harvested fractions was assessed on cellulosic substrates. [less ▲]

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See detailLe statut du logos. Compréhension, explicitation, énonciation
Gauvry, Charlotte ULg

in Revue Philosophique de Louvain (2014), 12(3), 473-493

En adoptant une méthode de lecture rétrospective des paragraphes 31, 32 et 33 d’Être et temps de Heidegger, l’article entend souligner que le traitement que réserve le grand œuvre de 1927 au concept de ... [more ▼]

En adoptant une méthode de lecture rétrospective des paragraphes 31, 32 et 33 d’Être et temps de Heidegger, l’article entend souligner que le traitement que réserve le grand œuvre de 1927 au concept de logos est largement préparé par les cours antérieurs de Marbourg et de Fribourg. Même s’il est indéniable que Heidegger propose dès 1923-24 une lecture ontologique progressive des analyses aristotéliciennes sur le discours, le premier enjeu de notre relecture est de prouver que Heidegger présente, si ce n’est une « philosophie du langage » autonome, de nombreuses intuitions originales sur le langage. À rebours de la logique sémantique de son temps (et notamment de celle de son maître Rickert) et du modèle encore dominant chez Husserl, Heidegger refuse en effet de faire de l’énoncé prédicatif l’archétype du discours. [less ▲]

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See detailImproving dairy cow fertility using milk-based indicator traits
Bastin, Catherine ULg; Vandenplas, Jérémie ULg; Gengler, Nicolas ULg

in Proceedings of the 10th World Congress on Genetics Applied to Livestock Production (2014, August)

Improvement of dairy cow fertility by means of genetic selection has become increasingly important over the last decades. Because fertility traits are difficult to measure and have low heritabilities ... [more ▼]

Improvement of dairy cow fertility by means of genetic selection has become increasingly important over the last decades. Because fertility traits are difficult to measure and have low heritabilities, indicator traits are of interest to supplement the prediction of genetic merit for female fertility. This paper examines milk-based traits that could be potential predictor of fertility: changes in protein and fat composition, fat to protein ratio, urea, lactose, ketone bodies, and mid-infrared prediction of body energy traits. The pattern of genetic correlations between these traits and fertility over days in milk is likely related to the cow’s energy balance state. Furthermore, changes in milk fatty acid profile were demonstrated as good potential predictors of fertility. Finally, additional research is warranted to investigate the association over the lactation between fertility and changes in milk biomarkers, potentially predicted by mid-infrared analysis of milk. [less ▲]

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See detailCombining magnetic and seismic studies to constrain processes in massive stars
Neiner, C.; Degroote, P.; Coste, B. et al

in IAUS 302 - Magnetic Fields Throughout Stellar Evolution (2014, August)

The presence of pulsations influences the local parameters at the surface of massive stars and thus it modifies the Zeeman magnetic signatures. Therefore it makes the characterisation of a magnetic field ... [more ▼]

The presence of pulsations influences the local parameters at the surface of massive stars and thus it modifies the Zeeman magnetic signatures. Therefore it makes the characterisation of a magnetic field in pulsating stars more difficult and the characterisation of pulsations is thus required for the study of magnetic massive stars. Conversely, the presence of a magnetic field can inhibit differential rotation and mixing in massive stars and thus provides important constraints for seismic modelling based on pulsation studies. As a consequence, it is necessary to combine spectropolarimetric and seismic studies for all massive classical pulsators. Below we show examples of such combined studies and the interplay between physical processes. [less ▲]

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See detailAuroral emissions of Jupiter and Saturn and satellite footprints
Radioti, Aikaterini ULg

Conference (2014, August)

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See detail"Worte stürzen zerbrochen über die Straße." Zum Gerücht als Figur und Erzählverfahren in Wolfgang Koeppens "Jugend"
Leyh, Valérie ULg

in Wirkendes Wort (2014), 64(2), 231-246

In Wolfgang Koeppens Prosafragment "Jugend" (1976) wirkt das Gerücht als Erzählstrategie und poetologisches Prinzip. Gerüchte scheinen wegen ihrer Verbindung zur bürgerlichen Gesellschaft, gegen die sich ... [more ▼]

In Wolfgang Koeppens Prosafragment "Jugend" (1976) wirkt das Gerücht als Erzählstrategie und poetologisches Prinzip. Gerüchte scheinen wegen ihrer Verbindung zur bürgerlichen Gesellschaft, gegen die sich der Erzähler vehement richtet, zunächst von diesem wegzuführen, doch erweisen sie sich letztlich als Teil seines Erzählens und als Reflexionsfigur für Koeppens Schreibprozess. [less ▲]

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See detailCross-Modal Decoding of Neural Patterns Associated with Working Memory: Evidence for Attention-Based Accounts of Working Memory
Majerus, Steve ULg; Cowan, Nelson; Peters, Frédéric ULg et al

in Cerebral Cortex (2014)

Recent studies suggest common neural substrates involved in verbal and visual working memory (WM), interpreted as reflecting shared attention-based, short-term retention mechanisms. We used a machine ... [more ▼]

Recent studies suggest common neural substrates involved in verbal and visual working memory (WM), interpreted as reflecting shared attention-based, short-term retention mechanisms. We used a machine-learning approach to determine more directly the extent to which common neural patterns characterize retention in verbal WM and visual WM. Verbal WM was assessed via a standard delayed probe recognition task for letter sequences of variable length. Visual WM was assessed via a visual array WM task involving the maintenance of variable amounts of visual information in the focus of attention. We trained a classifier to distinguish neural activation patterns associated with high- and low-visual WM load and tested the ability of this classifier to predict verbal WM load (high–low) from their associated neural activation patterns, and vice versa. We observed significant between-task prediction of load effects during WM maintenance, in posterior parietal and superior frontal regions of the dorsal attention network; in contrast, between-task prediction in sensory processing cortices was restricted to the encoding stage. Furthermore, between-task prediction of load effects was strongest in those participants presenting the highest capacity for the visual WM task. This study provides novel evidence for common, attention-based neural patterns supporting verbal and visual WM. [less ▲]

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See detailÉvaluer la force de l’association
Bruyère, Olivier ULg; Boutron-Ruault, Marie-Christine

in Medi-Sphere (2014), 451

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See detailMulti-period vehicle loading with stochastic release dates
Arda, Yasemin ULg; Crama, Yves ULg; Kronus, David ULg et al

in EURO Journal on Transportation and Logistics (2014), 3(2), 93-119

This paper investigates a multi-period vehicle loading problem with stochastic information regarding the release dates of items to be transported. The deterministic version of the problem can be ... [more ▼]

This paper investigates a multi-period vehicle loading problem with stochastic information regarding the release dates of items to be transported. The deterministic version of the problem can be formulated as a large-scale set covering problem. Several heuristic algorithms are proposed to generate decision policies for the stochastic optimization model over a long rolling horizon. The resulting policies have been extensively tested on instances which display the main characteristics of the industrial case-study that motivated the research. The tests demonstrate the benefits of the multi-period stochastic model over simple myopic strategies. A simple and efficient heuristic is shown to deliver good policies and to be robust against errors in the estimation of the probability distribution of the release dates. [less ▲]

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See detailComparison and impact of waste heat recovery technologies on passenger car fuel consumption on a normalized driving cycle
Legros, Arnaud ULg; Guillaume, Ludovic ULg; Diny, Mouad et al

in Energies (2014)

The purpose of this article was to compare different waste heat recovery system technologies designed for automotive applications. A complete literature review is done and results in two comparative ... [more ▼]

The purpose of this article was to compare different waste heat recovery system technologies designed for automotive applications. A complete literature review is done and results in two comparative graphs. In the second part, simulation models are built and calibrated in order to assess the fuel consumption reduction that can be achieved on a real driving cycle. The strength of this article is that the models are calibrated using actual data. Finally, those simulations results are analyzed and the Rankine cycle and turbocompound are the two most profitable solutions. However the simulations of the turbocompound shows its limitations because the impact on the exhaust pressure drop is not taken into account in the assessment of the car fuel consumption. Fuel reduction of up to 6% could be achieved, depending on the driving cycle and the waste heat recovery technology. [less ▲]

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See detailScalar triplet flavored leptogenesis: a systematic approach
Aristizabal Sierra, Diego ULg; Dhen, Mikael; Hambye, Thomas

in Journal of Cosmology and Astroparticle Physics [= JCAP] (2014), 2014

Type-II seesaw is a simple scenario in which Majorana neutrino masses are generated by the exchange of a heavy scalar electroweak triplet. When endowed with additional heavy fields, such as right-handed ... [more ▼]

Type-II seesaw is a simple scenario in which Majorana neutrino masses are generated by the exchange of a heavy scalar electroweak triplet. When endowed with additional heavy fields, such as right-handed neutrinos or extra triplets, it also provides a compelling framework for baryogenesis via leptogenesis. We derive in this context the full network of Boltzmann equations for studying leptogenesis in the flavored regime. To this end we determine the relations which hold among the chemical potentials of the various particle species in the thermal bath. This takes into account the standard model Yukawa interactions of both leptons and quarks as well as sphaleron processes which, depending on the temperature, may be classified as faster or slower than the Universe Hubble expansion. We find that when leptogenesis is enabled by the presence of an extra triplet, lepton flavor effects allow the production of the B-L asymmetry through lepton number conserving CP asymmetries. This scenario becomes dominant as soon as the triplets couple more to leptons than to standard model scalar doublets. In this case, the way the B-L asymmetry is created through flavor effects is novel: instead of invoking the effect of L-violating inverse decays faster than the Hubble rate, it involves the effect of L-violating inverse decays slower than the Hubble rate. We also analyze the more general situation where lepton number violating CP asymmetries are present and actively participate in the generation of the B-L asymmetry, pointing out that as long as L-violating triplet decays are still in thermal equilibrium when the triplet gauge scattering processes decouple, flavor effects can be striking, allowing to avoid all washout suppression effects from seesaw interactions. In this case the amount of B-L asymmetry produced is limited only by a universal gauge suppression effect, which nevertheless goes away for large triplet decay rates. [less ▲]

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See detailA Remark on the Poroelastic Center of Dilation
Huynen, Alexandre ULg; Detournay, Emmanuel

in Journal of Elasticity (2014), 116(2), 189-206

In the original derivation due to Dougall (1897), the center of dilation is constructed from the superposition of orthogonal force dipoles. However, it can also be built by combining dislocation dipoles ... [more ▼]

In the original derivation due to Dougall (1897), the center of dilation is constructed from the superposition of orthogonal force dipoles. However, it can also be built by combining dislocation dipoles. While the two representations are equivalent in elasticity, the different nature of the force and dislocation dipoles leads to different transient behaviors in poroelasticity, when the response evolves from undrained to drained. Indeed, we show that the poroelastic solution of a center of dilation is obtained by superposing the corresponding elastic solution and either an instantaneous fluid sink or an instantaneous fluid source, depending on whether the center of dilation is treated as a singular stress or displacement discontinuity. A simple explanation to the instantaneous injection or withdrawal of a finite volume of fluid from/to the singularity is given by adopting an exploded view of the poroelastic center of dilation. The exploration of the drained, undrained and transient responses of this singularity also requires to clarify the close relationship between an instantaneous fluid source and a continuous fluid dodecapole, an object constructed by collapsing onto a point three orthogonal pairs of fluid dipoles. [less ▲]

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See detailTerrestrial OH nightglow measurements during the Rosetta flyby
Migliorini, A.; Gérard, Jean-Claude ULg; Soret, Lauriane ULg et al

Conference (2014, August)

During the Rosetta travel to the comet P67, the VIRTIS instrument on board the spacecraft acquired unique images of the Earth in the 1.0-5.1 μm simultaneously. These allowed to investigate the nightglow ... [more ▼]

During the Rosetta travel to the comet P67, the VIRTIS instrument on board the spacecraft acquired unique images of the Earth in the 1.0-5.1 μm simultaneously. These allowed to investigate the nightglow observed spectrum and infer the relative OH populations of levels from 1 to 9. The values are reported in the table. The results are in good agreement with the model from GRANADA code (Kaufmann et al., 2008) for the conditions of a midlatitude night atmosphere. [less ▲]

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See detailWhich P inputs are compatible with a sustainable agriculture at short and long-term?
Renneson, Malorie ULg; Dufey, Joseph; Roisin, Christian et al

Poster (2014, August)

During the past 20 years, there has been a constant reduction in mineral fertilizer use due to price increases and environmental concerns. These changes can lead to a decrease in soil P content, which is ... [more ▼]

During the past 20 years, there has been a constant reduction in mineral fertilizer use due to price increases and environmental concerns. These changes can lead to a decrease in soil P content, which is already observed in some regions in Wallonia. Some new issues are now emerging. Is current cropping systems compatible with yield maintenance? Do organic fertilizers have a similar effect than mineral fertilizers? To answer to these questions, a short-term experiment in controlled conditions and 2 long-term experimental plots were studied. The short-term experiment permitted to study the kinetics of P after an input and differences between fertilizer types, whereas the long-term experiments studied 3 levels of P and K input and different organic compounds. Although an evolution of P content was observed, no difference of yield was found before about 20 years. However, after 47 years, available P levels were considered as low in zero P-input plots and attention must now be focused on these parcels. Zero P-input caused a mean yield decrease of 7%, while a double input increased yield by 2% in comparison to plots with input corresponding to crop export. Thus the zero P-input option is rarely economically profitable in the long-term and providing double the amount of P removed is never financially sustainable. Finally, no difference of P content was observed between organic and mineral fertilizers, except for manure which engendered a higher P content. In conclusion, organic and inorganic fertilizers had a relatively similar effect and overlooking P fertilizer is possible in the short-term but P content has to be followed at the long-term, although yield loss was limited. [less ▲]

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See detailPrediction of stock and fate of phosphorus forms according to soil classification
Renneson, Malorie ULg; Barbieux, Sophie ULg; Dufey, Joseph et al

Poster (2014, August)

Wallonia presents a high diversity of soils and the fate of P in the soil-plant systems can highly vary from one region to another. The fate of phosphorus depends upon its forms in the solid constituents ... [more ▼]

Wallonia presents a high diversity of soils and the fate of P in the soil-plant systems can highly vary from one region to another. The fate of phosphorus depends upon its forms in the solid constituents of soils, which is seldom characterized. For example, total P determines the soil reserve of P but also the potential P content which can be lost to surface water by erosion but analysis of this parameter is time consuming and rarely performed. This study aims (i) to define functional groups of soils for a differentiate P management, (ii) to estimate total soil P by regression equations based on soil parameters, and (iii) to estimate the quality of these predictions. The study consists in a characterization of 12 parent materials in Wallonia, collected across different land uses. A classification of soils was defined by clustering analysis and 5 groups were defined according to P contents and forms. Using this information in regression improved the quality of predictions. The coefficients of determination vary from 0.83 to 0.99, in comparison to a coefficient of 0.77 for the global regression. Then, pedotransfer functions were validated with an independent external dataset of 55 soils. Estimation of the quality of the prediction of P content (mean error, standard deviation of prediction and root mean square error) was made with global and local regression models. In conclusion, using a soil classification allowed to improve P content assessment by specific regressions and to propose differentiated P management for each group of soils. [less ▲]

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See detailDiversity patterns of the vascular plant group Zosterophyllopsida
Cascales - Miñana, Borja ULg; Meyer-Berthaud, Brigitte

Conference (2014, August)

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See detailRapport d'étude convention ISSEP-ULg PArtim 1 Echantillonnage de sources surfaciques d'odeur / Habay
Romain, Anne-Claude ULg; Adam, Gilles ULg; Molitor, Noémie ULg et al

Report (2014)

L'objectif de l'échantillonnage et de la mesure sur une source surfacique d'odeur est de déterminer le flux d'odeur surfacique (Specific Odour Emission Rate = SOER), en uo/m2.s, qui sera par la suite ... [more ▼]

L'objectif de l'échantillonnage et de la mesure sur une source surfacique d'odeur est de déterminer le flux d'odeur surfacique (Specific Odour Emission Rate = SOER), en uo/m2.s, qui sera par la suite multiplié par la surface totale d'émission pour obtenir le débit global d'odeur (Odour Emission Rate = OER), en uo/s. Dans le cas présent, il s'agissait surtout de comparer différents types de dispositifs d'échantillonnage et également d'évaluer la variabilité temporelle à court terme de l'émission (à différentes heures de la journée). Nous avons choisi de travailler sur un andain de compost de déchets verts en tout début de fermentation. Une première mesure a été effectuée le 21 juin 2013, à trois moments de la journée : vers 10h30 ("matin"), vers 12h30 ("midi") et vers 15h30 ("après-midi"). Une deuxième mesure a été réalisée le 10 juillet 2013 à deux moments de la journée, vers 11h00 ("matin") et vers 15h00 ("après-midi"). Une troisième a été réalisée le 19 septembre le matin à 11h00 (2 répétitions) [less ▲]

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