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See detailOptimiaztion of an electrophoretic approach for the screening and the development of new antithrombotic drugs
Farcas, Elena ULiege; Bouckaert, Charlotte; Servais, Anne-Catherine ULiege et al

Poster (2017, June 19)

The discovery of lead compounds that can modulate the activity of a biological target is essential to provide efficient pharmacological tools and to serve as starting points for new drug generations ... [more ▼]

The discovery of lead compounds that can modulate the activity of a biological target is essential to provide efficient pharmacological tools and to serve as starting points for new drug generations. Fragment-based drug discovery (FBDD) approach is an attractive tool for the identification of new selective inhibitors of a target of interest, but its success largely depends on the ability to develop screening bioassays capable to detect and gauge weak affinity binders. To achieve this goal, we investigated capillary electrophoresis (CE) for identifying and ranking fragments from an initial library. Indeed, due to its ability to evaluate weak interactions, CE seems to be promising for fragment-based screening. This technique is a powerful analytical tool with a unique separation mechanism, speed, efficiency and versatility. Its main advantages are low protein consumption, higher throughput compared to NMR and X-ray crystallography and the fact that screening can be carried out using native protein in physiological solution without the need of immobilization. We developed a proof of concept study on thrombin, a serine protease implicated in the coagulation cascade using affinity capillary electrophoresis (ACE) for ranking fragments from an initial library. For this study, we followed a probe ligand, benzamidine, and we investigated interactions with the target by monitoring the changes of its electrophoretic mobility upon binding. The first step of this study consisted in the optimization of the experimental conditions suitable for the CE method (target and probe ligand concentrations, separation buffer composition, voltage, separation effective length, target partial filling…). Then, numerous thrombin inhibitors with a wide range of inhibitory potency (i.e. Ki 200 µM – 5 nM) were tested to validate our system demonstrating the possibility to fish binders in the optimized conditions. We also checked the absence of non-specific binding with the target using the inactivated enzyme at the binding site. It is noteworthy that in this operating system (ACE assay), binding occurs in free solution using physiological buffers, thus preventing artifacts that may result from target immobilization, which is a requirement for some techniques such as SPR. [less ▲]

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See detailDesign and experimental validation of an adaptive control law to maximize power generation of a small-scale waste heat recovery system
Hernandez Naranjo, Jairo Andrés ULiege; Desideri, Adriano ULiege; Gusev, Sergei et al

in Applied Energy (2017), 203

Increasing the energy efficiency of industrial processes is a challenge that involves, not only improving the methodologies for design and manufacturing, but optimizing performance during part-load ... [more ▼]

Increasing the energy efficiency of industrial processes is a challenge that involves, not only improving the methodologies for design and manufacturing, but optimizing performance during part-load operation and transient conditions. A well-adopted solution consists of developing waste heat recovery (WHR) systems based on Organic Rankine Cycle (ORC) power units. The highest efficiency for such cycle is obtained at low superheating values, corresponding to the situation where the system exhibits time-varying nonlinear dynamics, triggered by the fluctuating nature of the waste heat source. In this paper, an adaptive control law using the Model Predictive Control (MPC) framework is proposed. This work goes a step beyond most of the existing scientific works in the field of ORC power systems, since the MPC controller is implemented in a lab-scale prototype, and its performance compared against a gain-scheduled PID strategy. The experimental results show that the adaptive MPC outperforms the gain-scheduled PID based strategy, as it allows to accurately regulate the evaporating temperature, while keeping vapor condition at the inlet of the expander i.e., the superheating, in a safe operating range, thus increasing the net power generation. [less ▲]

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See detailDEVELOPMENT AND VALIDATION OF A FAST SFC METHOD FOR THE ANALYSIS OF FLAVONOIDS IN PLANT EXTRACTS
Huang, Yang ULiege; Jiang, Zhengjin; Fillet, Marianne ULiege et al

Poster (2017, June 18)

Flavonoids from plants always show a wide range of biological activities [1-2]. In the present study, a rapid and highly efficient supercritical fluid chromatography (SFC) method was developed for the ... [more ▼]

Flavonoids from plants always show a wide range of biological activities [1-2]. In the present study, a rapid and highly efficient supercritical fluid chromatography (SFC) method was developed for the separation of 12 flavonoids. After careful optimization, the 12 flavonoids were baseline separated on a ZORBAX RX-SIL column using gradient elution. A 0.1% phosphoric acid solution in methanol was found to be the most suitable polar mobile phase component for the separation of flavonoids. From the viewpoint of retention and resolution, a backpressure of 200 bar and a temperature of 40 °C were shown to give the best results. Compared with a previously developed reverse phase liquid chromatography method, the SFC method could provide flavonoid separations that were about three times faster, while maintaining good peak shape and comparable peak efficiency. This SFC method was validated and applied to the analysis of five flavonoids (kaempferol, luteolin, quercetin, luteoloside, buddleoside) present in Chrysanthemum morifolium Ramat. from different cultivars (Chuju, Gongju, Hangju, Boju). The results indicated a good repeatability and sensitivity for the quantification of the five analytes with RSDs for overall precision lower than 3%. The limits of detection ranged from 0.73 to 2.34 μg/mL, while the limits of quantification were between 2.19 and 5.86 μg/mL. The method showed that SFC could be employed as a useful tool for the quality assessment of Traditional Chinese medicines (TCMs) containing flavonoids as active components. [less ▲]

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See detailPanel moderator: enforcement and remedies
Van Cleynenbreugel, Pieter ULiege

Scientific conference (2017, June 17)

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See detailENMG du pied (atelier)
WANG, François-Charles ULiege

Conference (2017, June 17)

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See detailNCA independence in the context of EU Regulation 1/2003: time to take incongruent accountability realities (more) seriously?
Van Cleynenbreugel, Pieter ULiege

Scientific conference (2017, June 16)

This paper argues that the Commission’s reform suggestions aimed at streamlining NCAs into independent administrative agencies appear to neglect this variety of accountability mechanisms underlying ... [more ▼]

This paper argues that the Commission’s reform suggestions aimed at streamlining NCAs into independent administrative agencies appear to neglect this variety of accountability mechanisms underlying current NCA institutional organisation and functioning. Given that those mechanisms each harbour incongruent expectations regarding the organisation and functioning of independent NCAs, failure to acknowledge such incongruence may in practice lead to less effective competition law enforcement. The purpose of this paper is therefore to uncover the different NCA independence perspectives underlying those accountability mechanisms and to look for legally sound ways to make them converge when designing or upgrading NCA independence in the context of the Regulation 1/2003 reform. [less ▲]

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See detailGeneralized Pascal triangles for binomial coefficients of finite words
Stipulanti, Manon ULiege

Conference (2017, June 16)

We introduce a generalization of Pascal triangle based on binomial coefficients of finite words. These coefficients count the number of times a finite word appears as a subsequence of another finite word ... [more ▼]

We introduce a generalization of Pascal triangle based on binomial coefficients of finite words. These coefficients count the number of times a finite word appears as a subsequence of another finite word. Similarly to the Sierpiński gasket that can be built as the limit set, for the Hausdorff distance, of a convergent sequence of normalized compact blocks extracted from Pascal triangle modulo 2, we describe and study the first properties of the subset of [0, 1] × [0, 1] associated with this extended Pascal triangle modulo a prime p. Then we create a new sequence from this extended Pascal triangle that counts, on each row of this triangle, the number of positive binomial coefficients. We show that this sequence is 2-regular. To extend our work, we construct a Pascal triangle using the Fibonacci representations of all the nonnegative integers and we define the corresponding sequence of which we study the regularity. This regularity is an extension of the classical k-regularity of sequences. [less ▲]

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See detailFirst Characterization of the Volatile Organic Compounds Emitted by Banana Plants (Musa sp.)
Berhal, Chadi ULiege; De Clerck, Caroline ULiege; LEVICEK, Carolina et al

Conference (2017, June 16)

Banana fruit (Musa sp.) ranks fourth in developing world's production, and has economical and nutritional key values. The popular and most dominant variety of the dessert banana group is the Cavendish ... [more ▼]

Banana fruit (Musa sp.) ranks fourth in developing world's production, and has economical and nutritional key values. The popular and most dominant variety of the dessert banana group is the Cavendish variety, and Plantain represents that status for the cooking banana group. Despite the importance of the Volatile Organic Compounds (VOCs), which are secondary metabolites with a high vapour pressure, in their utility in the plant protection and communication processes, they were never documented for the plant itself. Thus, the aim of this PHD thesis is to study the VOCs emitted by the plant, rather than their fruits or flowers. A protocol was optimized for the extraction of the banana plant's VOCs. The results of the first analysis showed 11 VOCs for the Cavendish, mainly (E,E)-α-farnesene (87.90 ± 11.28 ng/µl), methyl salicylate (33.82 ± 14.29) and 6-methyl-5-hepten-2-one (29.60 ± 11.66), and 14 VOCs for the Pacific Plantain cultivars, mainly (Z,E)-α-farnesene (799.64 ± 503.15),(E,E)-α-farnesene (571.24 ± 381.70) and (E) β ocimene (241.76 ± 158.49). Most of these compounds belong to the terpenes group (8 for Cavendish, 10 for Pacific Plantain). The other compounds detected were ketones, esters and aldehydes. Eight compounds were common between the two varieties (myrcene, Z and E β-ocimene, 6-methyl-5-hepten-2 one, 6-methyl-3,5-hepadien-2-one, a-farnesene, methyl salicylate and β-ionone). This exploratory study paves the way for an in-depth characterisation of VOCs emitted by Musa plants. [less ▲]

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Peer Reviewed
See detailRelevance of early stretching in overhead athletes
Schwartz, Cédric ULiege; Tubez, François ULiege; Croisier, Jean-Louis ULiege et al

Conference (2017, June 16)

Overhead athletes often develop, over time, a tightness of the posterior structures of the shoulder, which may be associated with injuries. Ten symptomatic (with pain) and ten asymptomatic players with a ... [more ▼]

Overhead athletes often develop, over time, a tightness of the posterior structures of the shoulder, which may be associated with injuries. Ten symptomatic (with pain) and ten asymptomatic players with a tight shoulder were compared and the effect of a self-applied stretching program was evaluated. Before and after the stretching program, pain and stiffness of the shoulder were evaluated. Our results demonstrate that risk factors for shoulder pain such as glenohumeral internal rotation deficit and total range of motion deficit may only be limited in symptomatic athletes. The mobility of the shoulder was significantly improved after the stretching program for both groups. Pain was reduced when present. Because of the limited differences between the symptomatic and asymptomatic athletes, clinicians may find it advantageous to initiate early prevention or rehabilitation programs. [less ▲]

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See detailFrammenti d’intonaci di I stile a Ostia Antica. Un lotto inedito proveniente dagli strati preparatori di un mosaico tardo-repubblicano (Domus dei Bucrani - Camera dei Nani)
Morard, Thomas ULiege; Motta, Lucie ULiege

Conference (2017, June 16)

Les fouilles entreprises durant les années 2002-2010 sur le site de la Schola del Traiano (IV, IV, 15) à Ostia Antica ont révélé l’existence d’une domus tardo-républicaine - la Domus aux Bucranes (60-20 ... [more ▼]

Les fouilles entreprises durant les années 2002-2010 sur le site de la Schola del Traiano (IV, IV, 15) à Ostia Antica ont révélé l’existence d’une domus tardo-républicaine - la Domus aux Bucranes (60-20 av. J.-C.) - dont le système décoratif de IIe style (pavements, enduits peints et reliefs de stuc, plafonds) est apparu exceptionnellement bien conservé dans les couches les plus profondes de la parcelle urbaine concernée. Ce système décoratif - actuellement en cours de publication - a déjà été présenté à la communauté scientifique lors de différents colloques et journées d’études internationales. Il n’est pas question d’y revenir dans le cadre de la prochaine rencontre de l’AIRPA. En revanche, nous souhaiterions y présenter un lot d’enduits peints de Ier style jetés pêle-mêle dans les couches de préparation du tapis de mosaïque de la Chambre des Nains - un oecus aménagé durant les années 40-30 au sein de cette même domus tardo-républicaine. Restés inédits jusqu’à ce jour, ces fragments comptent parmi les plus anciens jamais découverts à Ostia Antica et leur position stratigraphique - scellée entre deux pavements bien datés - offre à n’en pas douter matière à réflexion. L’ensemble de ces fragments a fait l’objet d’une documentation systématique et des analyses archéométriques ont été portées sur les mortiers et les pigments des éléments les plus significatifs. Ce sont les résultats de cette entreprise scientifique que nous souhaiterions présenter lors du colloque de l'Aquileia afin de pouvoir intégrer cet ensemble décoratif de Ier style dans un corpus plus large - une mise en relation étroite avec le matériel contemporain découvert dans le Latium. [less ▲]

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See detailElaboration of an aqueous sol-gel method for the synthesis of g-Al2O3 supports
Lambert, Stéphanie ULiege

Conference (2017, June 16)

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See detailLe cartoon. Réflexions sur la culture populaire aux États-Unis
Moreau, Timothée ULiege

Conference (2017, June 16)

The aim of this paper is to propose an analysis of the cartoon and its place in popular culture from two different yet connected angles: the idea of "pop culture", linked to mass culture and its critique ... [more ▼]

The aim of this paper is to propose an analysis of the cartoon and its place in popular culture from two different yet connected angles: the idea of "pop culture", linked to mass culture and its critique/analysis by Adorno & Horkheimer and Benjamin ; and the idea of "folk culture", linked to the notion of grotesque as it is developped by Bakhtin. The main idea is that those two paths of analysis are connected through the notion of ambivalence and their link to political conceptions of a particular society and the culture that emerges from it. [less ▲]

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See detailLe point de vue onomasiologique – Réseaux de sens et cartes sémantiques
Winand, Jean ULiege

Scientific conference (2017, June 16)

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See detailDevelopment of an open-source algorithm based on inertial measurement units (IMU) of a smartphone to detect cattle grass intake and ruminating behaviors
Andriamandroso, Andriamasinoro ULiege; Lebeau, Frédéric ULiege; Beckers, Yves ULiege et al

in Computers & Electronics in Agriculture (2017), 139

In this paper, an open algorithm was developed for the detection of cattle’s grass intake and rumination activities. This was done using the widely available inertial measurement unit (IMU) from a ... [more ▼]

In this paper, an open algorithm was developed for the detection of cattle’s grass intake and rumination activities. This was done using the widely available inertial measurement unit (IMU) from a smartphone, which contains an accelerometer, a gyroscope, a magnetometer and location sensors signals sampled at 100 Hz. This equipment was mounted on 19 grazing cows of different breeds and daily video sequences were recorded on pasture of different forage allowances. After visually analyzing the cows’ movements on a calibration database, signal combinations were selected and thresholds were determined based on 1-s time windows, since increasing the time window did not increase the accuracy of detection. The final algorithm uses the average value and standard deviation of two signals in a two-step discrimination tree: the gravitational acceleration on x-axis (Gx) expressing the cows’ head movements and the rotation rate on the same x-axis (Rx) expressing jaw movements. Threshold values encompassing 95% of the normalized calibrated data gave the best results. Validation on an independent database resulted in an average detection accuracy of 92% with a better detection for rumination (95%) than for grass intake (91%). The detection algorithm also allows for characterization of the diurnal feeding activities of cattle at pasture. Any user can make further improvements, for data collected at the same way as the iPhone’s IMU has done, since the algorithm codes are open and provided as supplementary data. [less ▲]

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See detailThe "Death" of Restorative Justice Advisers in Belgian Prisons
Dubois, Christophe ULiege

Scientific conference (2017, June 15)

This presentation accounts for three dimensions enlightening 20 years of RJ within Belgian Prisons: the policy-making process, the policy-implementation process, and the policy and organizational ... [more ▼]

This presentation accounts for three dimensions enlightening 20 years of RJ within Belgian Prisons: the policy-making process, the policy-implementation process, and the policy and organizational embeddedness of RJ in Belgian prison settings. [less ▲]

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See detailImpact of 3’-sialyllactose and Bifidobacterium crudilactis on infant feces microbial composition and virulence modulation of Escherichia coli O157:H7, using the SHIME® gastrointestinal model
Bondue, Pauline ULiege

Conference (2017, June 15)

Bifidobacterium crudilactis, a bacterial species from bovine origin, growths on bovine milk oligosaccharides (BMO). Cell free spent media (CFSM) from B. crudilactis and 3’-sialyllactose (3’SL), a major ... [more ▼]

Bifidobacterium crudilactis, a bacterial species from bovine origin, growths on bovine milk oligosaccharides (BMO). Cell free spent media (CFSM) from B. crudilactis and 3’-sialyllactose (3’SL), a major BMO, modulated Escherichia coli O157:H7 virulence gene expressiona. In this study, the SHIME® gastrointestinal model was inoculated with 4 different treatments: 3’SL (ttm1), B. crudilactis (ttm2), 3’SL and B. crudilactis (ttm3) and CFSM from 3’SL and B. crudilactis culture (ttm4). In each section of the colon, samples were collected and analysed for short chain fatty acid (SCFA) concentration using HPLC, microbial populations using 16S rDNA metagenetic analysis and correlated with E. coli O157:H7 virulence gene expression. The results showed that SCFA levels were stable during the experiments. Metagenetic analysis showed a microbial diversity in transverse (TC) and descending colon (DC) close to feces, dominated by Bacteroides, Prevotella and Fusobacterium, while the ascending colon (AC) showed a different microbial diversity dominated by Veillonella. Ttm4 and ttm2 induced mainly a down-regulation of virulence genes: fliC in DC with ttm4, and luxS, stx1, qseA in AC, DC or TC with ttm2. Ttm1 also showed a down-regulation of fliC in DC, similar to the one observed with ttm4, but this was associated with an up-regulation of fliC and stx1 in AC or TC. Finally, ttm3 showed slight upregulation of ler, fliC and qseA in AC. These results show that ttm4 and ttm2 might have a positive effect against virulence expression of E. coli O157:H7. However, this trend has to be validated with the further replicates on the SHIME® system. [less ▲]

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See detailExtrême droite et démocratie: entre opposition et récupération
Debras, François ULiege

Conference given outside the academic context (2017)

Après une période de dédiabolisation et grâce à une base électorale croissante, de plus en plus de partis d'extrême droite européens se revendiquent aujourd'hui comme démocratiques. En écartant de la ... [more ▼]

Après une période de dédiabolisation et grâce à une base électorale croissante, de plus en plus de partis d'extrême droite européens se revendiquent aujourd'hui comme démocratiques. En écartant de la scène politique et médiatique leurs membres les plus violents, en respectant les procédures électorales et en proposant des programmes alternatifs vis-à-vis des partis traditionnels, les organisations d'extrême droite s'intégreraient durablement au sein de nos systèmes politiques jusqu'à se présenter, dans certains pays, comme les véritables représentants du peuple, les défenseurs des libertés individuelles et les promoteurs d'une véritable démocratie loin des bureaucrates et technocrates de Bruxelles, loin des élites politiques et économiques qui ont confisqué le pouvoir au citoyens. Liberté d'expression, laïcité, référendum, souveraineté populaire... Autant de valeurs, autant de principes qui jettent la confusion dans un débat censé opposer la démocratie à l'extrême droite. Entre opposition et récupération, quels sont donc les liens qu'entretiennent les partis d'extrême droite avec la démocratie? [less ▲]

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