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See detailETEROGENEITÀ GENETICA DI NOROVIRUS BOVINI IDENTIFICATI IN ITALIA
Di Felice, Eliabetta; Di Profio, Federica; Melegari, Irene et al

Poster (2014, June)

I norovirus (NoV) sono piccoli virus a RNA monocatenario appartenenti alla famiglia Caliciviridae. Sulla base dell’analisi di sequenza del gene ORF2 codificante per la proteina capsidica VP1, i NoV sono ... [more ▼]

I norovirus (NoV) sono piccoli virus a RNA monocatenario appartenenti alla famiglia Caliciviridae. Sulla base dell’analisi di sequenza del gene ORF2 codificante per la proteina capsidica VP1, i NoV sono attualmente classificati in sei genogruppi (G), con GI, GII e GIV responsabili di gastroenterite nell’uomo. Calicivirus enterici morfologicamente simili a NoV umani sono stati identificati per la prima volta in vitelli diarroici nel Regno Unito nel 1978 e in Germania nel 1980. Il sequenziamento nucleotidico dell’intero genoma ha permesso di classificare i NoV bovini (BoNoV) all’interno del genogruppo III in due genotipi, GIII.1 (prototipo Bo/Jena/80/DE) e GIII.2 (prototipo Bo/Newbury/76/UK). I BoNoV hanno una diffusione mondiale con una maggiore prevalenza di virus GIII.2-like. Tuttavia, studi recenti basati sull’analisi nucleotidica a livello della giunzione fra la polimerasi (RdRp) ed il capside, hanno dimostrato la circolazione di ceppi ricombinanti. Nel presente lavoro vengono riportati i risultati di un’indagine per la ricerca e tipizzazione di BoNoV condotta su quattro allevamenti bovini ubicati nelle province di Teramo e Pescara. A tal fine, 104 tamponi rettali collezionati da vitelli asintomatici sono stati sottoposti a nested RT-PCR impiegando due set di primer specifici per BoNoV in grado di amplificare un frammento di 326 bp della RdRp. L’RNA di BoNoV è stato identificato nel 10,5% (11/104) degli animali testati. L'analisi di sequenza ha evidenziato per nove sequenze un’elevata identità nucleotidica (nt) (88-96%) con i ceppi GIII.2-like, mentre per due è stata rilevata la maggiore identità (87-90% nt) con i BoNoV GIII.1-like. Per sette ceppi è stata ottenuta anche una sequenza di circa 750 bp che includeva oltre che la regione parziale della RdRp, la regione 5’ capsidica. Sulla base dell’analisi molecolare, cinque ceppi sono risultati strettamente correlati con BoNoV GIII.2-like, mentre solo uno dei due ceppi con polimerasi GIII.1-like, ha mostrato la maggiore identità nucleotidica nei confronti di BoNoV appartenenti al genotipo 1. Il ceppo 80/TE/IT che possedeva una polimerasi GIII.1-like, a livello capsidico ha mostrato la più alta identità con ceppi GIII.2-like, suggerendo un fenomeno di ricombinazione a livello della giunzione ORF1/ORF2. [less ▲]

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See detailSTUDIO in vitro DEI PARAMETRI DI COINFEZIONE E SUPERINFEZIONE CHE INFLUENZANO LA RICOMBINAZIONE NEI NOROVIRUS
Di Felice, Elisabetta; Ceci, Chiara; Zonta, William ULg et al

Poster (2014, June)

I norovirus (NoV) sono piccoli virus (38-40 nm) a RNA monocatenario sprovvisti di envelope appartenenti alla famiglia Caliciviridae. I NoV attualmente vengono riconosciuti come una delle più importanti ... [more ▼]

I norovirus (NoV) sono piccoli virus (38-40 nm) a RNA monocatenario sprovvisti di envelope appartenenti alla famiglia Caliciviridae. I NoV attualmente vengono riconosciuti come una delle più importanti cause di gastroenterite acute di origine non batterica nell'uomo coinvolgendo tutte le classi di età e soprattutto contesti comunitari. I meccanismi molecolari che influenzano l'evoluzione dei NoV sono l'accumulo di mutazioni puntiformi e la ricombinazione. L’obiettivo sarà quello di dimostrare che la ricombinazione è un evento che può essere influenzabile dall’impiego di diversi parametri di coinfezione/superinfezione. In letteratura, gli studi sugli eventi ricombinanti che caratterizzano i NoV umani risultano piuttosto limitati a causa dell'assenza di un substrato cellulare che ne permetta la replicazione in vitro. Nel presente lavoro è stato sviluppato un modello sperimentale basato sull’impiego dell’unico NoV, il norovirus murino (MNV), in grado di replicare su substrato cellulare. A tal fine monostrati di cellule RAW264.7 sono stati coinfettati e superinfettati con due ceppi di MNV (CW1 e WU20) utilizzando diversi indici di molteplicità (MOI) e differenti tempi di infezione. I surnatanti sono stati collezionati a 24 e 48 ore post-infezione e quindi sottoposti a metodo delle placche. All’interno della popolazione virale risultante da tale coinfezione/superinfezione, 36 cloni per ciascuna condizione sono stati selezionati e purificati dalle placche e, successivamente, utilizzati per infettare nuovi monostrati cellulari di RAW264.7. Il monitoraggio degli eventi ricombinanti è stato eseguito mediante PCR e Real-Time PCR dopo estrazione dell'RNA virale e retrotrascrizione, impiegando due set di primer in grado di amplificare le regioni site all’estremità iniziale di ORF1 e all’estremità terminale di ORF3. I risultati che permetteranno di quantificare la distribuzione dei ceppi presenti nel surnatante della superinfezione, sono ancora in corso di analisi. [less ▲]

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See detailThe role of segmentation and investor recognition through the lens of cross-listing activity
Mouchette, Xavier ULg; Muller, Aline ULg; Carrieri, Francesca

Conference (2014, June)

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See detailThe role of segmentation and investor recognition through the lens of cross-listing activity
Mouchette, Xavier ULg; Carrieri, Francesca; Muller, Aline ULg

Conference (2014, June)

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See detailThe role of segmentation and investor recognition through the lens of cross‐listing activity
Mouchette, Xavier ULg; Muller, Aline ULg; Carrieri, Francesca

Conference (2014, June)

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See detailAltération de la fonction rénale chez le patient âgé, comment gérer?
KRZESINSKI, Jean-Marie ULg; DELANAYE, Pierre ULg

in Revue Médicale de Liège (2014), 69(5-6), 287-293

From age 30 onwards, kidney function physiologically decreases although this deterioration cannot yet be called chronic kidney disease. The latter appears in those exposed to cardiovascular risk factors ... [more ▼]

From age 30 onwards, kidney function physiologically decreases although this deterioration cannot yet be called chronic kidney disease. The latter appears in those exposed to cardiovascular risk factors associated with inflammation and oxidative stress. A diffuse atherosclerosis then develops Patients with a decreased glomerular filtration rate, especially below the threshold of 45 ml/min, are characterised by a poor physical heath and by cognitive disorders, leading to frailty. In these conditions, a management strategy to reduce the increased risk of acute kidney injury should be outlined and the need for renal replacement therapy be considered. One must try to maintain the best possible quality of life, promoting in some situations a conservative approach. [less ▲]

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See detailPrise en charge de l'hypertension artérielle du patient âgé
XHIGNESSE, Patricia ULg; Saint-Remy, Annie ULg; Krzesinski, Jean-Marie ULg

in Revue Médicale de Liège (2014), 69(5-6), 294-300

High blood pressure is very frequent in the elderly; it represents a real threat for the patient’s health and a source of huge costs for the economic system. Systolic hypertension is the most frequent ... [more ▼]

High blood pressure is very frequent in the elderly; it represents a real threat for the patient’s health and a source of huge costs for the economic system. Systolic hypertension is the most frequent form observed in the old, due to large arteries stiffness. Antihypertensive therapy has proven effective to decrease significantly the cardiovascular morbi-mortality and total mortality in this population. A non pharmacological approach is also very useful, but should not be too restrictive. Blood pressure target in patients older than 65 (and, particularly, in octogenarians) is 150/80 mmHg. Blood pressure should be checked in the upright position before changing the drug dosage. The first line therapy in the old should generally be a calcium channel antagonist or a low dose diuretic. [less ▲]

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See detailLe ciel étoilé d’Isis et d’Héphaïstos à Memphis
Veymiers, Richard ULg

Conference (2014, June)

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See detailBelowground Chemical Ecology: The Case of Wireworms
Barsics, Fanny ULg; Delory, Benjamin ULg; Delaplace, Pierre ULg et al

Poster (2014, June)

Wireworms, clock-beetles' larvae (Coleoptera, Elateridae), are below ground pests of many crops. They cause dramatic yield losses worldwide. Research on their ecology is crucial to undertake innovative ... [more ▼]

Wireworms, clock-beetles' larvae (Coleoptera, Elateridae), are below ground pests of many crops. They cause dramatic yield losses worldwide. Research on their ecology is crucial to undertake innovative management strategies. In the field of chemical ecology, multitrophic interactions occurring in the rhizosphere are gaining increasing attention from entomologists and agronomists. Our research aims at unveiling the role of volatile organic compounds (VOC) involved in wireworms' foraging behavior, putatively leading to host localization and/or host recognition. [less ▲]

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See detailA window on exoplanet dynamical histories: Rossiter-McLaughlin observations of WASP-13b and WASP-32b
Brothwell, R.D.; Watson, C.A.; Hébrard, G. et al

in Monthly Notices of the Royal Astronomical Society (2014), 440(4), 3392-3401

We present Rossiter-McLaughlin observations of WASP-13b and WASP-32b and determine the sky-projected angle between the normal of the planetary orbit and the stellar rotation axis (lambda). WASP-13b and ... [more ▼]

We present Rossiter-McLaughlin observations of WASP-13b and WASP-32b and determine the sky-projected angle between the normal of the planetary orbit and the stellar rotation axis (lambda). WASP-13b and WASP-32b both have prograde orbits and are consistent with alignment with measured sky-projected angles of lambda =8°^{+13}_{-12} and lambda =-2°^{+17}_{-19}, respectively. Both WASP-13 and WASP-32 have Teff < 6250 K, and therefore, these systems support the general trend that aligned planetary systems are preferentially found orbiting cool host stars. A Lomb-Scargle periodogram analysis was carried out on archival SuperWASP data for both systems. A statistically significant stellar rotation period detection (above 99.9 per cent confidence) was identified for the WASP-32 system with Prot = 11.6 ± 1.0 days. This rotation period is in agreement with the predicted stellar rotation period calculated from the stellar radius, R*, and vsin i if a stellar inclination of i* = 90° is assumed. With the determined rotation period, the true 3D angle between the stellar rotation axis and the planetary orbit, psi, was found to be psi = 11° ± 14°. We conclude with a discussion on the alignment of systems around cool host stars with Teff < 6150 K by calculating the tidal dissipation time-scale. We find that systems with short tidal dissipation time-scales are preferentially aligned and systems with long tidal dissipation time-scales have a broad range of obliquities. [less ▲]

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See detailThe grassland model intercomparison of the MACSUR (Modelling European Agriculture with Climate Change for Food Security) European knowledge hub
Ma, Shaoxiu; Acutis, Marco; Barcza, Zoltan et al

in Proceedings of the 7th International Congress on Environmental Modelling and Software (iEMSs) June 15-19, 2014, San Diego, California, USA (2014, June)

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See detailSynthèse bibliographique sur la mobilité des éléments traces dans les sols et l’utilisation de la stabilisation physico-chimique comme technique de gestion in situ des sites contaminés
Liénard, Amandine ULg; Colinet, Gilles ULg

in Biotechnologie, Agronomie, Société et Environnement = Biotechnology, Agronomy, Society and Environment [=BASE] (2014), 18(2), 273-289

Industrial activities have deeply impacted the quality of biophysical environment at places. This review focuses on consequences of soil contaminations by metallic trace elements: what conditions the ... [more ▼]

Industrial activities have deeply impacted the quality of biophysical environment at places. This review focuses on consequences of soil contaminations by metallic trace elements: what conditions the mobility of contaminants in soils and what are the remediation practices based upon physico-chemical stabilization? This technique is an in situ remediation which aims at limiting the dispersion of metals in the environment by use of soil conditioners and amendments in contaminated sites. First of all, we reviewed the concepts of mobility of trace elements and their indicators. Then, we compiled the different kinds of amendments applied to stabilize contaminants in soils and the processes involved. [less ▲]

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See detailStereoselective synthesis of peptidoglycan fragments on solid support
Simon, Justine ULg; Lamborelle, Nicolas ULg; Joris, Bernard ULg et al

Poster (2014, June)

The mammalian proteins, Nod1 and Nod2, are able to recognize peptidoglycan motifs during bacterial infection. Interestingly, Nod1 is a detector of peptides and muropeptides containing the meso-2,6 ... [more ▼]

The mammalian proteins, Nod1 and Nod2, are able to recognize peptidoglycan motifs during bacterial infection. Interestingly, Nod1 is a detector of peptides and muropeptides containing the meso-2,6-diaminopimelic acid (m-A2pm), a non-proteinogenic amino acid found in Gram- bacteria. In this work, we describe a synthesis of peptidoglycan motifs entirely on solid support. Solid-Phase Peptide Synthesis (SPPS), Cross Metathesis (CM) and sugar chemistry are three methods to anchor respectively amino acids, m-A2pm and sugars derivatives on a resin as solid support. [less ▲]

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See detailPosterior Cingulate Cortex-Related Co-Activation Patterns: A Resting State fMRI Study in Propofol-Induced Loss of Consciousness
Amico, Enrico ULg; Gomez, Francisco; Di Perri, Carol et al

in PLoS ONE (2014), 9

Background: Recent studies have been shown that functional connectivity of cerebral areas is not a static phenomenon, but exhibits spontaneous fluctuations over time. There is evidence that fluctuating ... [more ▼]

Background: Recent studies have been shown that functional connectivity of cerebral areas is not a static phenomenon, but exhibits spontaneous fluctuations over time. There is evidence that fluctuating connectivity is an intrinsic phenomenon of brain dynamics that persists during anesthesia. Lately, point process analysis applied on functional data has revealed that much of the information regarding brain connectivity is contained in a fraction of critical time points of a resting state dataset. In the present study we want to extend this methodology for the investigation of resting state fMRI spatial pattern changes during propofol-induced modulation of consciousness, with the aim of extracting new insights on brain networks consciousness-dependent fluctuations. Methods: Resting-state fMRI volumes on 18 healthy subjects were acquired in four clinical states during propofol injection: wakefulness, sedation, unconsciousness, and recovery. The dataset was reduced to a spatio-temporal point process by selecting time points in the Posterior Cingulate Cortex (PCC) at which the signal is higher than a given threshold (i.e., BOLD intensity above 1 standard deviation). Spatial clustering on the PCC time frames extracted was then performed (number of clusters = 8), to obtain 8 different PCC co-activation patterns (CAPs) for each level of consciousness. Results: The current analysis shows that the core of the PCC-CAPs throughout consciousness modulation seems to be preserved. Nonetheless, this methodology enables to differentiate region-specific propofol-induced reductions in PCC-CAPs, some of them already present in the functional connectivity literature (e.g., disconnections of the prefrontal cortex, thalamus, auditory cortex), some others new (e.g., reduced co-activation in motor cortex and visual area). Conclusion: In conclusion, our results indicate that the employed methodology can help in improving and refining the characterization of local functional changes in the brain associated to propofol-induced modulation of consciousness. [less ▲]

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See detailAnalysis versus synthesis for trending of gas-path measurement time series
Borguet, Sébastien ULg; Léonard, Olivier ULg; Dewallef, Pierre ULg

in Proceedings of ASME Turbo Expo 2014 (2014, June)

Gas-path measurements used to assess the health condition of an engine are corrupted by noise. Generally, a data cleaning step occurs before proceeding with fault detection and isolation. Classical linear ... [more ▼]

Gas-path measurements used to assess the health condition of an engine are corrupted by noise. Generally, a data cleaning step occurs before proceeding with fault detection and isolation. Classical linear filters such as the exponentially weighted moving average filter are traditionally used for noise removal. Unfortunately, these low-pass filters distort trend shifts indicative of faults, which increases the detection delay. The present paper investigates two new approaches to non-linear filtering of time series. On one hand, the synthesis approach reconstructs the signal as a combination of elementary signals chosen from a pre-defined library. On the other hand, the analysis approach imposes a constraint on the shape of the signal (e.g., piecewise constant). Both approaches incorporate prior information about the signal in a different way, but they lead to trend filters that are very capable at noise removal while preserving at the same time sharp edges in the signal. This is highlighted through the comparison with a classical linear filter on a batch of synthetic data representative of typical engine fault profiles. [less ▲]

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See detailÉtude des changements de circulation au-dessus de l'océan Austral en été
Belleflamme, Alexandre ULg; Fettweis, Xavier ULg; Erpicum, Michel ULg

in Camberlin, Pierre; Richard, Yves (Eds.) Actes du XXVIIème colloque de l'Association de Climatologie - Climat : système & interactions (2014, June)

Over the five last decades, the reanalyses (ERA and NCEP/NCAR) show a strengthening of the pressure gradient between the southern hemisphere subtropical anticyclone belt and the southern circumpolar lows ... [more ▼]

Over the five last decades, the reanalyses (ERA and NCEP/NCAR) show a strengthening of the pressure gradient between the southern hemisphere subtropical anticyclone belt and the southern circumpolar lows during summer. With the help of an automatic circulation type classification, we show that the strengthening of the pressure gradient is generalised to all circulation types and, paradoxically, it does not cause circulation changes. It is probably implied by the strengthening of the temperature gradient between the tropics and the South Pole, without consequences on the general circulation. Our classification also allows a successful comparison between the two reanalyses in a region where the observation data are rare. [less ▲]

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See detailLe "péril jaune" en RDC : mythe ou réalité ?
Wintgens, Sophie ULg; Kabamba, Bob ULg

in Santander, Sébastian (Ed.) L’Afrique, nouveau terrain de jeu des émergents (2014)

L’influence de la Chine en république démocratique du Congo (RDC) se caractérise par son caractère tardif mais fulgurant, au regard d’une présence chinoise historiquement forte en Afrique subsaharienne ... [more ▼]

L’influence de la Chine en république démocratique du Congo (RDC) se caractérise par son caractère tardif mais fulgurant, au regard d’une présence chinoise historiquement forte en Afrique subsaharienne. Son implantation offensive en RDC résulte d’une convergence d’intérêts entre un État chinois en quête de matières premières et de terres arables pour assurer son développement économique et sa stabilité politique, et un État congolais en (re)construction résolument acquis, depuis l’élection du président Joseph Kabila, au modèle de développement chinois fondé sur la séparation volontaire entre objectifs économiques et réformes politiques (prêts inconditionnés, aide déliée). En consacrant l’accroissement rapide des relations commerciales sino-congolaises, le partenariat stratégique Chine-Afrique, scellé en 2006 lors du troisième Forum de coopération sino-africain, participe plus largement de la remise en cause du rôle des puissances traditionnellement influentes dans la région. Au-delà de la concurrence économique induite vis-à-vis des États-Unis, du Japon et des institutions financières internationales au sein desquelles elle est largement représentée, l’Europe assiste plus singulièrement à la remise en question de son leadership régional et à la dépréciation normative de sa politique développementale. Entre émancipation et hégémonie, cet article vise à mettre au jour les enjeux stratégiques inhérents à l’impact du "facteur chinois" en RDC. [less ▲]

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See detailPlatelet-rich plasma to treat patellar tendinopathies: a 1 year follow-up.
Kaux, Jean-François ULg; Croisier, Jean-Louis ULg; Bruyère, Olivier ULg et al

in Clinical Chemistry & Laboratory Medicine (2014, June), 52(Special Suppl), 1285

Background: Jumper’s knee is a frequent chronic overuse syndrome of the upper part of the patellar tendon. Platelets contain lots of growth factors which could enhance the healing process of tendons ... [more ▼]

Background: Jumper’s knee is a frequent chronic overuse syndrome of the upper part of the patellar tendon. Platelets contain lots of growth factors which could enhance the healing process of tendons. Infiltration of Platelet Rich Plasma (PRP) may be considered as a recent therapeutic option for chronic tendinopathies. The aim of the current study is to evaluate the clinical status and the return to sports activities in patients with chronic upper patellar tendinopathies up to 1 year after 1 infiltration of PRP. Methods: Twenty patients with chronic upper patellar tendinopathy were enrolled. Assessments were made before infiltration of PRP, and 6 weeks and 3 months and 1 year after the infiltration, using a 10-point Visual Analogic Scale and algofunctional scores (IKDC and VISA-P). Moreover, they had to answer an information questionnaire concerning their life and sports activities. The PRP was obtained with an apheresis system (COM.TEC, Fresenius). Six millilitres of PRP were injected without local anaesthetic. One week after infiltration, patients started a standardised sub-maximal eccentric reeducation. Results: Pain during daily activities significantly decreased with time. VAS has significantly dropped, IKDC and VISA-P significantly improved over the follow-up of 1 year. Seventy percents of patients reported a favourable evolution with decrease of pain, 15% did never report any improvement and 15% were treated surgically. Seventy percents returned to sports activities, 64,3% without any pain, and 50% of them recovered the same sport level. Younger patients seemed to be more susceptible to have an improvement of pain by the PRP infiltration. Conclusions: This study demonstrates that a local infiltration of PRP associated with a submaximal eccentric protocol can improve, at 1 year, symptoms of chronic jumper’s knee in patients non-responsive to classical conservative treatments. [less ▲]

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