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See detailRapid prenatal diagnosis of fetal Zellweger syndrome by biochemical tests, complementation studies, and molecular analyses.
Segers, Karin; Pierquin, Genevieve; Gaillez, Stephanie et al

in Prenatal Diagnosis (2013), 33(2), 201-3

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See detailReproductive and productive performances of the stress-negative Piétrain pigs in the tropics: the case of Vietnam
Do Duc, Luc ULg; Ha Xuan, Bo; Thomson, Peter et al

in Animal Production Science (2013), 53(2), 173-179

The objective of the present study is to investigate the reproduction, production and semen traits of the stressnegative Piétrain line of pigs in the north of Vietnam, as well as to identify various ... [more ▼]

The objective of the present study is to investigate the reproduction, production and semen traits of the stressnegative Piétrain line of pigs in the north of Vietnam, as well as to identify various factors (halothane genotype, generationorigin, parity, sex and month of the year) acting on these traits. The experiment was carried out on a purebred nucleus on Dong Hiep farm, Haiphong province in Vietnam. The reproduction performances and the sperm quality measures were based on 12 sows and 5 boars imported from Belgium as well as on 29 sows and 14 boars born in Vietnam, while the production performances were recorded only from 251 animals born in Vietnam. No significant differences were observed between halothane genotypes of sows for reproduction traits (P>0.0578). At the age of 7.5 months, bodyweight, backfat thickness and longissimus depth (7.5) of homozygous-dominant pigs were higher (P<0.05) than in heterozygotes. Homozygous-dominant boars had higher total number of spermatozoa than heterozygote boars (P < 0.05); similarly, Vietnam boars also presented higher total number of spermatozoa values than Belgium boars (P < 0.001). Vietnam sows had longer duration of pregnancy, more piglets born alive and heavier litter weight of weaning than Belgium sows (P < 0.01). Bodyweight at birth, at weaning, at 2, 5.5 and 7.5 months of age increased from the first to third parity (P < 0.05). Gilts had more lean meat than intact boars (P < 0.01). Compared with observations made in temperate countries, the reproduction and production performances, sperm concentration and total number of spermatozoa of stress-negative Piétrain pigs were reduced, whereas lean meat percentage was not affected. This might be due to the different climate, although improving values for animals born in Vietnam might indicate adaptation of the animals and/or of the technicians with that breed. The results support the interest of the use of this new Piétrain line in tropical countries with ecological and climatic conditions similar to Vietnam. [less ▲]

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See detailDevelopment and aging of the Kisspeptin-GPR54 system in the mammalian brain: what are the impacts on female reproductive function?
Franceschini, Isabelle; Desroziers, Elodie ULg

in Frontiers in Neuroendocrine Science (2013)

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See detailHydrolysis of starches and flours by sorghum malt amylases for dextrins production
Ba, Khady ULg; Aguedo, Mario ULg; Tine, Emmanuel et al

in European Food Research & Technology (2013)

Corn and wheat starches as well as wheat and cassava flours were hydrolyzed using sorghum malt at 65 °C for 6 hours. During these reactions, dextrose equivalent (DE) values were followed under 3 ... [more ▼]

Corn and wheat starches as well as wheat and cassava flours were hydrolyzed using sorghum malt at 65 °C for 6 hours. During these reactions, dextrose equivalent (DE) values were followed under 3 concentrations of sorghum malt and of calcium chloride. Wheat flour presented the highest DE values and cassava flour had the highest hydrolysis yield. Thus, different dextrins were produced in a pilot plant and were analyzed by HPSEC and HPAEC-PAD for their molecular weight distribution and oligosaccharides composition respectively. The results indicated that oligosaccharides with broad molecular weight distributions were present in the dextrins produced and that the proportion of maltose was very high. [less ▲]

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See detailMiR-210 promotes a hypoxic phenotype and increases radioresistance in human lung cancer cell lines.
Grosso, S.; Doyen, J.; Parks, S. K. et al

in Cell Death & Disease (2013), 4

The resistance of hypoxic cells to radiotherapy and chemotherapy is a major problem in the treatment of cancer. Recently, an additional mode of hypoxia-inducible factor (HIF)-dependent transcriptional ... [more ▼]

The resistance of hypoxic cells to radiotherapy and chemotherapy is a major problem in the treatment of cancer. Recently, an additional mode of hypoxia-inducible factor (HIF)-dependent transcriptional regulation, involving modulation of a specific set of micro RNAs (miRNAs), including miR-210, has emerged. We have recently shown that HIF-1 induction of miR-210 also stabilizes HIF-1 through a positive regulatory loop. Therefore, we hypothesized that by stabilizing HIF-1 in normoxia, miR-210 may protect cancer cells from radiation. We developed a non-small cell lung carcinoma (NSCLC)-derived cell line (A549) stably expressing miR-210 (pmiR-210) or a control miRNA (pmiR-Ctl). The miR-210-expressing cells showed a significant stabilization of HIF-1 associated with mitochondrial defects and a glycolytic phenotype. Cells were subjected to radiation levels ranging from 0 to 10 Gy in normoxia and hypoxia. Cells expressing miR-210 in normoxia had the same level of radioresistance as control cells in hypoxia. Under hypoxia, pmiR-210 cells showed a low mortality rate owing to a decrease in apoptosis, with an ability to grow even at 10 Gy. This miR-210 phenotype was reproduced in another NSCLC cell line (H1975) and in HeLa cells. We have established that radioresistance was independent of p53 and cell cycle status. In addition, we have shown that genomic double-strand breaks (DSBs) foci disappear faster in pmiR-210 than in pmiR-Ctl cells, suggesting that miR-210 expression promotes a more efficient DSB repair. Finally, HIF-1 invalidation in pmiR-210 cells removed the radioresistant phenotype, showing that this mechanism is dependent on HIF-1. In conclusion, miR-210 appears to be a component of the radioresistance of hypoxic cancer cells. Given the high stability of most miRNAs, this advantage could be used by tumor cells in conditions where reoxygenation has occurred and suggests that strategies targeting miR-210 could enhance tumor radiosensitization. [less ▲]

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See detailFlow-cytometric assessment of damages to Acetobacter senegalensis during freeze-drying process and storage
Shafiei, Rasoul ULg; Delvigne, Frank ULg; Thonart, Philippe ULg

in Acetic Acid Bacteria (2013)

Downstream processes have great influences on bacterial starter production. Different modifications occur to cellular compounds during freeze-drying process and storage of bacterial starters. Consequently ... [more ▼]

Downstream processes have great influences on bacterial starter production. Different modifications occur to cellular compounds during freeze-drying process and storage of bacterial starters. Consequently, viability and culturability (multiplication capacity) undergo some changes. In this study, the effects of freeze-drying process and storage conditions were examined on cell envelope integrity, respiration and culturability of Acetobacter senegalensis. Freezing of cells protected with mannitol (20% w/w) did not affect cell multiplication and respiration considerably; however, 19% of cells showed compromised cell envelope after freezing. After drying, 1.96×1011 CFU/g were enumerated, indicating that about 34% of the cells could survive and keep their culturability. Drying of the cells induced further leakage in cell envelope and finally 81% of cells appeared as injured ones; however, 87% of the dried cells maintained their respiration capacity. Storage temperature had significant effect on cell multiplication ability; higher storage temperature (35°C) caused 8.59-log reduction in cell culturability after nine-month period of storage. Collapse of cell envelop integrity and respiration was observed at 35°C. At lower storage temperature (4°C), the culturability decreased about one-log reduction after nine months. Cell envelope integrity was subjected to minor changes during a period of nine month-storage at 4°C whereas a heterogeneous population of cells with different respiration capacity emerged at 4°C. These results indicate that a major part of cells undergone drying process and storage entered into viable but non-culturable state. In addition, usage of different culture media didn’t improve resuscitation. Besides, it seems that sub-lethal damages to cell envelope caused uptake of propidium iodide, however these kinds of injuries could not impress cell multiplications and respiration. [less ▲]

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See detailStepped spillway Downstream of a Piano Key Weir - Critical length for uniforme flow
Silvestri, Aurélien ULg

in Bung, Daniel B.; Pagliara, Stefano (Eds.) International Workshop on Hydraulic Design of Low-Head Structures (2013)

Recent researches showed the rapid aeration of the flow downstream of piano key weirs. This could be related to the specific geometric features of the structure, creating several interacting flows and ... [more ▼]

Recent researches showed the rapid aeration of the flow downstream of piano key weirs. This could be related to the specific geometric features of the structure, creating several interacting flows and jets downstream. In order to validate this assumption, an experimental research has been carried out at the Laboratory of Engineering Hydraulics, University of Liège. The main objective of the study was to an-alyze the energy dissipation occurring in skimming flow conditions on a stepped spillway downstream of a piano key weir (PKW). Comparison with energy dissipation on a stepped spillway downstream of a standard ogee crested weir has been performed. An indirect method has been used to determine the resid-ual energy at the spillway toe. The results show that uniform flow conditions are reached faster on a stepped spillway downstream of a PWK compared to the length needed downstream of a standard ogee crested weir. Extrapolation of the results to prototype flow conditions is also discussed with regards to scale effects influence. [less ▲]

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See detailInventaire des archives de la famille Nagelmackers
Oger, Cécile ULg; Caulier-Mathy, Nicole; Haesenne-Peremans, Nicole

Report (2013)

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See detailLa Littérature à l'ombre. Sociologie du Zutisme.
Saint-Amand, Denis ULg

Book published by Classiques Garnier (2013)

Durant les derniers mois de 1871, une microsociété d’écrivains et artistes liés à la Bohème parisienne prend ses quartiers à l’Hôtel des Étrangers. En secret, les membres de ce groupuscule forment un ... [more ▼]

Durant les derniers mois de 1871, une microsociété d’écrivains et artistes liés à la Bohème parisienne prend ses quartiers à l’Hôtel des Étrangers. En secret, les membres de ce groupuscule forment un cercle, le Zutisme, et prennent part à la confection d’un manuscrit libertaire et potache, l’Album zutique, qu’ils remplissent de sonnets monosyllabiques, dizains parodiques et autres caricatures scabreuses. Les auteurs de ces textes frondeurs sont Rimbaud, Verlaine et Cros, mais aussi Valade, Cabaner et Keck. Le présent livre revient sur la genèse et les logiques de cette éphémère aventure, sur la conduite de vie collective qui s’y est développée, sur les enjeux et effets, enfin, de l’œuvre qui en est le produit. [less ▲]

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See detailMobility and distribution of lead, cadmium, copper and zinc in soil profiles in the peri-urban market garden of Kunming, Yunnan Province, China
Zu, Yanqun; Bock, Laurent ULg; Schvartz, Christian et al

in Archives of Agronomy and Soil Science (2013)

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See detailLevels of polychlorinated dibenzo-p-dioxins, polychlorinated dibenzofurans and polychlorinated biphenyls in human milk from different regions of France
Focant, Jean-François ULg; Fréry, N; Bidondo, ML et al

in Science of the Total Environment (2013), 452-453

Wereport on the pilot study carried out before the start of the Elfe project (French longitudinal study from childhood). A total of 44 samples of mature human milk were collected at home 8 weeks after ... [more ▼]

Wereport on the pilot study carried out before the start of the Elfe project (French longitudinal study from childhood). A total of 44 samples of mature human milk were collected at home 8 weeks after delivery. A total of 7 polychlorinated dibenzo-p-dioxins (PCDDs), 10 polychlorinated dibenzofurans (PCDFs), 12 dioxin-like (DL) polychlorinated biphenyls (PCBs), and 6 non dioxin-like (NDL)-PCBs were measured. For total TEQ (PCDD/Fs and DL-PCBs), the geometric mean concentration was 17.81 pg TEQWHO05/g lipids. Relative PCDD, PCDF, and DL-PCB contributions to the arithmetic mean TEQWHO05 were 38%, 18%, and 44%, respectively. The use of TEFWHO05 instead of TEFWHO98 resulted in a 27% reduction of the total TEQ value. Although PCDD levels did not significantly change (less than 0.5% increase), PCDF and DL-PCB levels both decreased by 35% and 38%, respectively. Levels have been compared to data obtained during a previous non-reported national study conducted in 1998 (TEFWHO98) in French lactaria (n = 244). The mean of PCDD/Fs has decreased about 39.4% (18.8 pg TEQWHO98/g lipids in 1998 vs 11.4 pg TEQWHO98/g lipids in pilot study), respectively 41.5% for PCDDs (10.6 pg TEQWHO98/g lipids in 1998 vs 6.2 pg TEQWHO98/g lipids in pilot study) and 36.7% for PCDFs (7.9 pg TEQWHO98/g lipids in 1998 vs 5.0 pg TEQWHO98/g lipids in pilot study). For the sum of the 6 NDL-PCBs, the 2007 geometric mean concentration in milk was 176.3 ng/g lipids. The arithmetic mean lipid concentration in 2007 breast milk was 26.4 g/l (range from 6.0 to 46.7 g/l). A PCDD/F and DL-PCB daily intake was estimated to be 62.3 pg TEQWHO05/kg body weight per day (85.0 pg TEQWHO98/kg bodyweight per day) for a baby of 5 kg of bodyweight fed daily with 700 ml of maternal milk containing 25 g/l of lipids. [less ▲]

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See detailToward genomic prediction from whole-genome sequence data: impact of sequencing design on genotype imputation and accuracy of predictions.
Druet, Tom ULg; Macleod, I. M.; Hayes, B. J.

in Heredity (2013)

Genomic prediction from whole-genome sequence data is attractive, as the accuracy of genomic prediction is no longer bounded by extent of linkage disequilibrium between DNA markers and causal mutations ... [more ▼]

Genomic prediction from whole-genome sequence data is attractive, as the accuracy of genomic prediction is no longer bounded by extent of linkage disequilibrium between DNA markers and causal mutations affecting the trait, given the causal mutations are in the data set. A cost-effective strategy could be to sequence a small proportion of the population, and impute sequence data to the rest of the reference population. Here, we describe strategies for selecting individuals for sequencing, based on either pedigree relationships or haplotype diversity. Performance of these strategies (number of variants detected and accuracy of imputation) were evaluated in sequence data simulated through a real Belgian Blue cattle pedigree. A strategy (AHAP), which selected a subset of individuals for sequencing that maximized the number of unique haplotypes (from single-nucleotide polymorphism panel data) sequenced gave good performance across a range of variant minor allele frequencies. We then investigated the optimum number of individuals to sequence by fold coverage given a maximum total sequencing effort. At 600 total fold coverage (x 600), the optimum strategy was to sequence 75 individuals at eightfold coverage. Finally, we investigated the accuracy of genomic predictions that could be achieved. The advantage of using imputed sequence data compared with dense SNP array genotypes was highly dependent on the allele frequency spectrum of the causative mutations affecting the trait. When this followed a neutral distribution, the advantage of the imputed sequence data was small; however, when the causal mutations all had low minor allele frequencies, using the sequence data improved the accuracy of genomic prediction by up to 30%.Heredity advance online publication, 3 April 2013; doi:10.1038/hdy.2013.13. [less ▲]

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See detailDioxin levels in fertilizers from Belgium: Determination and evaluation of the potential impact on soil contamination
Elskens, M; Pussemier, L; Dumortier, P et al

in Science of the Total Environment (2013), 454-455

Dioxins are harmful Persistent Organic Pollutants (POPs) to which humans are exposed mostly via the consumption of animal products. They can enter the food chain at any stage, including crop fertilization ... [more ▼]

Dioxins are harmful Persistent Organic Pollutants (POPs) to which humans are exposed mostly via the consumption of animal products. They can enter the food chain at any stage, including crop fertilization. Fertilizers belong to several categories: synthetic chemicals providing the essential elements (mostly N, P and K) that are required by the crops but also organic fertilizers or amendments, liming materials, etc. Ninety-seven samples of fertilizers were taken in Belgium during the year 2011 and analyzed after a soft extraction procedure for polychlorinated dibenzo-p-dioxins (PCDDs), polychlorinated dibenzofurans (PCDFs) and dioxin-like polychlorinated biphenyls (DL-PCBs) using GC-IDHRMS. Only small qualitative differences could be observed between the main fertilizer categories since the PCDD:PCDF:DL-PCB average ratio obtained with the results expressed in WHO 2005-TEQ was often close to 30:30:40 (typically for sewage sludge) or 40:30:30 (typically for compost). The median dioxin levels determined were generally lower than recorded previously and were the highest for sewage sludge and compost (5.6 and 5.5 ng TEQ/kg dry weight (dw), respectively). The levels in other fertilizers were lower including manure for which the median value was only 0.2 ng TEQ/kg dw. Several fertilization scenarios relying on the use of those fertilizers were assessed taking into consideration the application conditions prevailing in Belgium. From this assessment it could be concluded that the contribution of fertilizers to the overall soil contamination will be low by comparison of other sources of contamination such as atmospheric depositions. At the field scale, intensive use of compost and sewage sludge will increase dramatically the dioxin inputs compared with other fertilization practices but this kind of immission to the soil will still be relatively low compared to the dioxin atmospheric depositions. [less ▲]

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See detailArt on the lagoon: Eliasson at the Biennale of Venice
Szanto, Catherine ULg; Gay, Fabrizio; Ballarin, Matteo

in Newman, Conor; Nussaume, Yann; Pedroli, Bas (Eds.) Landscape and Imagination. Towards a new baseline for education in a changing world (2013)

The Biennales of Venice are not limited to exhibitions in national pavilions. Every year artists and architects choose to confront the site and more specifically, the lagoon. In the edition of 2005, the ... [more ▼]

The Biennales of Venice are not limited to exhibitions in national pavilions. Every year artists and architects choose to confront the site and more specifically, the lagoon. In the edition of 2005, the artist O. Eliasson proposed an installation (Your black horizon, 2005) where the visitors left the wide expanse and intense light of the lagoon to enter a darkened enclosed space with subtle light effects. These aesthetic experiences enhanced the visitors’ perception of light by changing the usual spatiality and temporality of colour. We will analyze the role of memory in the rite of the visit, and look at how the museal strategies of the artist create a play with spatial memory and sense of orientation in relation to the outdoor space of the lagoon. [less ▲]

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See detailLa tolérance est coûteuse. On essaye l'intolérance ?
Jacquemain, Marc ULg

E-print/Working paper (2013)

Le texte a servi à présenter une conférence à des étudiants de bacheliers pédagogiques. Il rappelle, sur un ton informel, mais en essayant d'être rigoureux, la place et l'importance de la tolérance dans ... [more ▼]

Le texte a servi à présenter une conférence à des étudiants de bacheliers pédagogiques. Il rappelle, sur un ton informel, mais en essayant d'être rigoureux, la place et l'importance de la tolérance dans toute conception raisonnable de la vie en société. Il rappelle notamment que si la tolérance n'est pas une vertu "flamboyante", elle constitue un préalable indispensable à d'autres modalités plus exigeantes du vivre ensemble [less ▲]

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See detailLong-Term Care Insurance and Family Norms
Pestieau, Pierre ULg; Canta, Chiara

in The B.E. Journal of Economic Analysis & Policy (2013), 14(1), 1-28

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See detailAnalytical determination of stress patterns in the periodontal ligament during orthodontic tooth movement.
Van Schepdael, An ULg; Geris, Liesbet ULg; Vander Sloten, Jos

in Medical Engineering & Physics (2013), 35(3), 403-10

A dedicated software package that allows simulation of tooth movement can lead to shortening of the treatment program in orthodontics. A first step in the development of this software is the modelling of ... [more ▼]

A dedicated software package that allows simulation of tooth movement can lead to shortening of the treatment program in orthodontics. A first step in the development of this software is the modelling of the movement of a single tooth. Forces applied to the crown of the tooth are transmitted to the alveolar bone through the periodontal ligament, stretching, and compressing the ligament, eventually resulting in tooth movement. This paper presents an analytical model that predicts stresses and strains inside this ligament by approximating the shape of the root as an elliptic paraboloid. The model input consists of 2 material parameters and 4 geometrical parameters. To assess the accuracy of the model a finite element model (FEM) was constructed to compare the results and the influence of the eccentricity of the root was studied. The results show that the model is able to successfully describe the global behavior of the PDL and, except at a region near the alveolar crest, the differences between analytical and FEM results are small. In contrast to FEM, the analytical model does not require setting up a 3D-model and creating a mesh, allowing for significantly lower computational times and reducing cost when implementing in clinical practice. [less ▲]

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See detailMechanisms of cell migration in the adult brain: modelling subventricular neurogenesis.
Van Schepdael, An ULg; Ashbourn, J. M. A.; Beard, R. et al

in Computer Methods in Biomechanics & Biomedical Engineering (2013)

Neurogenesis has been the subject of active research in recent years. Although the majority of neurons form during the embryonic period, neurogenesis continues in restricted regions of the mammalian brain ... [more ▼]

Neurogenesis has been the subject of active research in recent years. Although the majority of neurons form during the embryonic period, neurogenesis continues in restricted regions of the mammalian brain well into adulthood. In rodent brains, neuronal migration is present in the rostral migratory stream (RMS), connecting the subventricular zone to the olfactory bulb (OB). The migration in the RMS is characterised by a lack of dispersion of neuroblasts into the surrounding tissues and a highly directed motion towards the OB. This study uses a simple mathematical model to investigate several theories of migration of neuroblasts through the RMS proposed in the literature, including chemo-attraction, chemorepulsion, general inhibition and the presence of a migration-inducing protein. Apart from the general inhibition model, all the models were able to provide results in good qualitative correspondence with the experimental observations. [less ▲]

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