Last 7 days     Results 841-860 of 67403.   38 39 40 41 42 43 44 45 46 47 48   Let's create, not debate. Co-creation as the ‘new wave’ of public engagement? First insights from WalloniaMacq, Hadrien Scientific conference (2017, July 04)Detailed reference viewed: 62 (2 ULg) For your ka! How to give something as a present to the tomb owner?Motte, Aurore Conference (2017, July 04)Detailed reference viewed: 23 (2 ULg) Maximal voluntary isometric contraction tests for normalizing surface EMG of scapular stabilizers musclesSchwartz, Cédric ; Tubez, François ; Soulier, Maxime et alConference (2017, July 04)Detailed reference viewed: 15 (5 ULg) Comparative Proteomics Analysis Provides New Candidates for Zinc Homeostasis Regulation in ArabidopsisAmini, Sahand ; Arsova, Borjana ; Scheepers, Maxime et alPoster (2017, July 03)Zinc (Zn) is an essential micronutrient for plants and around two billion people are depending on grains and legumes as their main Zn source. On the other hand, this transition metal is toxic for plants ... [more ▼]Zinc (Zn) is an essential micronutrient for plants and around two billion people are depending on grains and legumes as their main Zn source. On the other hand, this transition metal is toxic for plants at high concentrations in soils. This calls for a better unravelling of Zn homeostasis regulation mechanisms, including sensing and signaling in plants. In order to fulfill this aim, we are testing for novel proteins involved in Zn homeostasis in the model plant Arabidopsis thaliana. First, quantitative proteomics was performed on root and shoot samples obtained upon Zn starvation and re-supply in different spatio-temporal conditions. Inductively coupled plasma atomic emission spectroscopy (ICP-AES) analysis was also performed for those treatments to measure the Zn concentration in tissues. It showed very rapid Zn uptake in root upon re-supply. Moreover, quantitative expression studies of known players of Zn homeostasis confirmed our large-scale proteomic results, although for a few genes lack of correlation between transcript and protein regulation was observed. Using clustering, statistical and gene ontology analyses, we selected candidate genes for further studies. Among more than 5000 detected proteins in roots by shotgun proteomics, 75 genes were selected for targeted analyses. In general, our results show that comparative proteomics study can be useful to reveal new players in the Zn regulatory network in plants, which can lead to new Zn biofortification and phytoremediation strategies. [less ▲]Detailed reference viewed: 29 (8 ULg) Characterizing Thiamine Status and Mechanisms of Thiamine Supplementation on Subacute Ruminal Acidosis Attenuation in Dairy CowsPan, Xiaohua Doctoral thesis (2017)Dairy cows are often fed high grain diets to maximize milk production in today’s intensive management farms. However, overfeeding high grain diets increases the risk of subacute ruminal acidosis (SARA ... [more ▼]Dairy cows are often fed high grain diets to maximize milk production in today’s intensive management farms. However, overfeeding high grain diets increases the risk of subacute ruminal acidosis (SARA), which is characterized by prolonged decrease in rumen pH and high levels of lipopolysaccharide (LPS). SARA challenge has been confirmed to severely impair animal health, production performance and farm profitability, and more and more attentions have been paid to prevent the occurrence of SARA in dairy industry worldwide. Our teams’ previous research found that thiamine supplementation relieved SARA in dairy cows, but the modes of action of thiamine in SARA attenuation are still unclear. In this context, this thesis was conducted to increase our understanding of relationships between SARA induction and thiamine, and to explore the mechanisms of thiamine supplementation on SARA attenuation by determining its effects on rumen fermentation, microbiome composition and anti-inflammatory response. Firstly, the first experiment in Chapter III demonstrated that thiamine status in rumen and blood were altered by SARA induction and thiamine deficiency occurred during SARA challenge. Regression analysis proved the ruminal thiamine content was positively related to pH and the concentrations of acetate in the rumen, and negatively correlated to the lactate contents, indicating the altered rumen fermentation would affect thiamine status. Secondly, the mRNA expression of thiamine transporters in ruminal epithelium (Chapter IV) and ruminal bacteria compositions (Chapter V) in SARA and control cows were detected. We found that thiamine concentration was positively correlated with Bacteroides, Ruminococcus 1, Ruminobacter, Pyramidobacter and Fibrobacter, and the decrease in these genera implied SARA challenge altered ruminal thiamine status by inhibiting the growth of thiamine synthesis related bacteria. The positive correlation between ruminal and blood thiamine, and the down-regulation of thiamine transporters’ expression in rumen epithelium indicated the reduced ruminal thiamine synthesis and thiamine absorption by SARA challenge resulted in low blood thiamine content of SARA cows. Thirdly, the effects of thiamine on rumen fermentation (Chapter III), anti-inflammatory response (Chapter IV) and microbiome composition (Chapter V) were evaluated to reveal its mechanisms on SARA attenuation. We found that thiamine supplementation promoted acetate-producing bacteria including Ruminococcus 1, Pyramidobacter, Succinivibrio and Bacteroides, and decreased bacteria positively related to ruminal lactate (Succiniclasticum and Ruminococcaceae NK4A214). Consequently, rumen fermentation was improved by reducing the accumulation of lactate and increasing ruminal pH. In addition, thiamine supplementation alleviated inflammatory response in rumen epithelium by reducing the release of LPS and phosphorylation of NFκB protein, which is conducive to SARA attenuation. In summary, this thesis had increased our understanding of thiamine nutrition in dairy cows, and provided a new control strategy for subacute ruminal acidosis in dairy cows. However, further investigations are needed to deeply understand the relationship between thiamine and SARA induction, such as the isolation and validation of thiamine synthesis bacteria affected by SARA challenge, as well as to figure out metabolic pathways through which SARA induction affects thiamine synthesis in the rumen. Moreover, the impacts of overfeeding high grain diets on intestinal thiamine absorption are still unclear and need to be illuminated in dairy cows. [less ▲]Detailed reference viewed: 25 (5 ULg) Benchmarking the Environmental Impact of Green and Traditional Masonry Wall ConstructionsGauvreau-Lemelin, Corinne; Attia, Shady in Roaf, Susan (Ed.) Passive low energy architecture design to thrive (2017, July 03)In Belgium, the most common approach for nearly Zero Energy Buildings is to comply with the locally modified version of the German Passive House (PH) Standard that requires a very low conductivity of ... [more ▼]In Belgium, the most common approach for nearly Zero Energy Buildings is to comply with the locally modified version of the German Passive House (PH) Standard that requires a very low conductivity of exterior walls. The conventional PH brick constructions are dominated by building materials with high environmental impact including concrete blocks, firebrick and petrochemical insulation materials that produce a great amount of greenhouse gases (GHG). Moreover, there are very few studies that assessed the holistic environmental impact of conventional wall compositions against ecological wall compositions. Therefore, this research compares a traditional Belgian representative wall against a hemp block wall, according to the PH standard. The environmental impact of each wall is quantified through a life cycle assessment. The final results indicate that the hemp wall reinforced with a wood skeleton has a much lower impact on the environment: up to 60% reduction on total primary energy, 72% on climate changes, 93% on eutrophication, 61 % on ozone layer depletion and 74% on acidification. Future work may refine the assessment process. The study provides novel and significant findings that can inform building owners, architects and contractors and encourage them to choose environmentally friendly masonry wall compositions. [less ▲]Detailed reference viewed: 20 (3 ULg) Omics insights into rumen ureolytic bacterial community and urea metabolism in dairy cowsJin, Di Doctoral thesis (2017)Urea has been used in the diets of ruminants as a non-protein nitrogen source. Ureolytic bacteria are key organisms in the rumen producing urease enzymes to catalyze the breakdown of urea to ammonia (NH3 ... [more ▼]Urea has been used in the diets of ruminants as a non-protein nitrogen source. Ureolytic bacteria are key organisms in the rumen producing urease enzymes to catalyze the breakdown of urea to ammonia (NH3), and the NH3 is used as nitrogen for microbial protein synthesis. In the rumen, hydrolysis of urea to NH3 occurs at a greater rate than NH3 can be utilized by rumen bacteria, and excess ammonia absorbed into blood may be harmful to the animals. Nowadays, little is known about the information of ureolytic microorganisms in the rumen, and the changes that occur in the rumen microbial and host metabolites induced by urea nitrogen have not been fully characterized. ‘Omics’ approaches, such as metagenomics and metabolomics have been applied to analyzing rumen microbial community and nutrients metabolism in dairy cows. The objective of this study is to investigate the rumen predominant ureolytic bacteria community and the mechanisms of urea utilization in ruminants using sequencing and metabolomics approaches. Firstly, an in vitro experiment trying to explore the ruminal ureolytic bacterial community was performed. Urea or acetohydroxamic acid were supplemented into the rumen simulation systems as the stimulator and inhibitor for ureolytic bacteria, respectively. The bacterial 16S rRNA genes were analyzed by Miseq sequencing and used to reveal the ureolytic bacteria by comparing different treatments. We found that urea supplementation significantly increased the proportion of ureC genes. The rumen ureolytic bacteria were abundant in the genera of Pseudomonas, Haemophilus, Neisseria, Streptococcus, Actinomyces, Bacillus and unclassified Succinivibrionaceae. Secondly, an in vivo experiment was taken to investigate differences in ureolytic bacterial composition between the rumen digesta and rumen wall based on ureC gene classification. Six dairy cows with rumen fistula were assigned to a two-period cross-over trial. One group was fed a total mixed ration without urea and the treatment group was fed rations plus 180 g urea per cow per day. Rumen bacterial samples from rumen content and rumen wall fractions were collected for ureC gene amplification and sequencing using Miseq. More than 55% of the ureC sequences did not affiliate with any known taxonomically assigned urease genes. The wall-adherent bacteria had a distinct ureolytic bacterial profile compared to the bacteria in the rumen content. The most abundant ureC genes were affiliated with Methylococcaceae, Clostridiaceae, Paenibacillaceae, Helicobacteraceae and Methylophilaceae families. Relative abundance of the operational taxonomic units (OTUs) affiliated with Methylophilus and Marinobacter genera were significantly higher in the bacteria on the rumen wall than that in the rumen content. Thirdly, based on the in vivo experiment, rumen fluid and blood samples were collected and analyzed using nuclear magnetic resonance spectroscopy and multivariate analysis of variance. Concentrations of valine, aspartate, glutamate, and uracil in the rumen, and urea and pyroglutamate in the plasma were increased after urea supplementation. Metabolic pathways include pantothenate and CoA biosynthesis, beta-alanine metabolism, valine, leucine, and isoleucine metabolism in the rumen, and urea and glutathione metabolism in the plasma were significantly increased by urea nitrogen. In conclusion, this study identified significant populations of ureolytic bacterial community that have not been recognized or studied previously in the rumen and provides a basis for obtaining regulatory targets to moderate urea hydrolysis in the rumen. The findings also provided novel information to aid understanding of the metabolic pathways affected by urea nitrogen in dairy cows, and could potentially help to guide efforts directed at improving the efficiency of urea utilization in the rumen. [less ▲]Detailed reference viewed: 32 (5 ULg) Histoire évolutive du complexe Afzelia Smith (Leguminosae - Caesalpinioideae) dans les écosystèmes forestiers et savanicoles en Afrique tropicaleDonkpegan, Segbedji Doctoral thesis (2017)The genus Afzelia Smith (Fabaceae, Caesalpinioideae) is known to have seven African tree species two of which are found in the Zambezian region, one is distributed in the Sudanian region and the four ... [more ▼]The genus Afzelia Smith (Fabaceae, Caesalpinioideae) is known to have seven African tree species two of which are found in the Zambezian region, one is distributed in the Sudanian region and the four other are endemic to the Guineo-Congolian region. These taxa, of high commercial value, are difficult to identify. They are therefore marketed under the same "doussié" name. These difficulties of distinction can prove detrimental to the sustainable management of populations. The aim of this doctoral thesis is to characterize the evolutionary history of the Afzelia genus. More specifically, this study aims to: (i) evaluate the level of morphological divergences within the Afzelia genus and describe the phylogenetic relationships in order to quantify the reproductive isolation between taxa by highlighting the role of past climate change and / or ecological gradients in the speciation of the genus; (ii) proceed to an analysis of the spatial genetic diversity and structure of Afzelia spp.; (iii) identify and describe ecological, biotic and abiotic factors that may influence population-level gene flows of an Afzelia species (A. bipindensis). A morpho-genetic analysis of Afzelia species was carried out and confirmed the strong botanical resemblance between the taxa. The savannah species are diploid and have half the size of the genome of forest species that are tetraploid. The phylogenies of genes (nuclear and chloroplastic) differ from one another and do not allow the separation of tetraploid taxa from one another. Such differences can be generated as a result of episodes of ancestral hybridization between species. These hybridizations would probably be old and would have occurred between lineages of the forest species and A quanzensis (a species of the Zambezian savannahs) lineages. Polyploidy would have occurred between 7 and 9.4 million years in the evolutionary history of the genus. In addition, Bayesian assignment and reproductive isolation analyzes suggested interspecific crosses, but only in forest species distributed sympatrically. At a more limited spatial scale, we observed two well differentiated genetic groups in sympatry in A. bipindensis. These show a morphological differentiation and a phenological shift of flowering which can contribute to their reproductive isolation. This study highlighted some important points: the discovery of a polyploid complex within the Afzelia genus, the confirmation of the delimitation of the diploid savannah species and the need to revise the delimitation of forest tetraploid species. [less ▲]Detailed reference viewed: 39 (10 ULg) Ion Mobility-Mass Spectrometry: Going beyond the numbersHaler, Jean ; Far, Johann ; Béchet, Eric et alScientific conference (2017, July 03)Detailed reference viewed: 16 (2 ULg) Vibration Induced Phenomena in Granular Media in MicrogravityOpsomer, Eric ; Noirhomme, Martial ; Vandewalle, Nicolas Poster (2017, July 03)Detailed reference viewed: 20 (2 ULg) H0LiCOW - I. H0 Lenses in COSMOGRAIL's Wellspring: program overviewSuyu, S. H.; Bonvin, V.; Courbin, F. et alin Monthly Notices of the Royal Astronomical Society (2017), 468Strong gravitational lens systems with time delays between the multiple images allow measurements of time-delay distances, which are primarily sensitive to the Hubble constant that is key to probing dark ... [more ▼]Strong gravitational lens systems with time delays between the multiple images allow measurements of time-delay distances, which are primarily sensitive to the Hubble constant that is key to probing dark energy, neutrino physics and the spatial curvature of the Universe, as well as discovering new physics. We present H0LiCOW (H[SUB]0[/SUB] Lenses in COSMOGRAIL's Wellspring), a program that aims to measure H[SUB]0[/SUB] with <3.5 per cent uncertainty from five lens systems (B1608+656, RXJ1131-1231, HE 0435-1223, WFI2033-4723 and HE 1104-1805). We have been acquiring (1) time delays through COSMOGRAIL and Very Large Array monitoring, (2) high-resolution Hubble Space Telescope imaging for the lens mass modelling, (3) wide-field imaging and spectroscopy to characterize the lens environment and (4) moderate-resolution spectroscopy to obtain the stellar velocity dispersion of the lenses for mass modelling. In cosmological models with one-parameter extension to flat Λ cold dark matter, we expect to measure H[SUB]0[/SUB] to <3.5 per cent in most models, spatial curvature Ω[SUB]k[/SUB] to 0.004, w to 0.14 and the effective number of neutrino species to 0.2 (1σ uncertainties) when combined with current cosmic microwave background (CMB) experiments. These are, respectively, a factor of ˜15, ˜2 and ˜1.5 tighter than CMB alone. Our data set will further enable us to study the stellar initial mass function of the lens galaxies, and the co-evolution of supermassive black holes and their host galaxies. This program will provide a foundation for extracting cosmological distances from the hundreds of time-delay lenses that are expected to be discovered in current and future surveys. [less ▲]Detailed reference viewed: 17 (2 ULg) New Media Criticism and the Body: Hans Magnus Enzensberger’s Ambivalent Reflection on Digital DevicesHamers, Jérémy Conference (2017, July 01)Detailed reference viewed: 22 (0 ULg) Trade unions and political culture(s) in the mining area of KolweziGeenen, Kristien Conference (2017, July 01)Detailed reference viewed: 66 (1 ULg) Probing the atmosphere of a sub-Jovian planet orbiting a cool dwarfSedaghati, Elyar; Boffin, Henri M. J.; Delrez, Laetitia et alin Monthly Notices of the Royal Astronomical Society (2017), 468We derive the 0.01 $\mu$m binned transmission spectrum, between 0.74 and 1.0 $\mu$m, of WASP-80b from low resolution spectra obtained with the FORS2 instrument attached to ESO's Very Large Telescope. The ... [more ▼]We derive the 0.01 $\mu$m binned transmission spectrum, between 0.74 and 1.0 $\mu$m, of WASP-80b from low resolution spectra obtained with the FORS2 instrument attached to ESO's Very Large Telescope. The combination of the fact that WASP-80 is an active star, together with instrumental and telluric factors, introduces correlated noise in the observed transit light curves, which we treat quantitatively using Gaussian Processes. Comparison of our results together with those from previous studies, to theoretically calculated models reveals an equilibrium temperature in agreement with the previously measured value of 825K, and a sub-solar metallicity, as well as an atmosphere depleted of molecular species with absorption bands in the IR ($\gg 5\sigma$). Our transmission spectrum alone shows evidence for additional absorption from the potassium core and wing, whereby its presence is detected from analysis of narrow 0.003 $\mu$m bin light curves ($\gg 5\sigma$). Further observations with visible and near-UV filters will be required to expand this spectrum and provide more in-depth knowledge of the atmosphere. These detections are only made possible through an instrument-dependent baseline model and a careful analysis of systematics in the data. [less ▲]Detailed reference viewed: 10 (0 ULg) Changes in cannabis use in emerging adulthood: The influence of peer network, impulsivity, anxiety and depressionGlowacz, Fabienne ; Schmits, Emilie in European Review of Applied Psychology = Revue Européenne de Psychologie Appliquée (2017), 67(4), 171-179Cannabis is the most commonly used illicit drug with the highest prevalence reported among 15- to 24-year-olds. This specific period of emerging adulthood constitutes a critical age for substance use and ... [more ▼]Cannabis is the most commonly used illicit drug with the highest prevalence reported among 15- to 24-year-olds. This specific period of emerging adulthood constitutes a critical age for substance use and for future consumption. Cannabis use patterns change during college or university and the post-college transition; users are at greater risk of adverse health outcomes (especially if they start or maintain a pattern of frequent use). Objectives The overall aim of this study was to highlight psychological and relational factors that might be associated with changes (including cessation and fluctuation) in cannabis use during this specific period, separately for males and females. Methods The subjects were 682 first-year college students (69.94% of female), aged between 18 and 25 years (M = 18.59 years, SD = 1.56). Four groups were formed according to cannabis consumption: “non-users” (54.64%), “desisting users” (14.04%), “fluctuating users” (16.23%), and “persistent users” (15.07%). A self-report questionnaire was administered to evaluate prevalence, frequency and trajectory of use, number of peer cannabis users, alcohol use, impulsivity, anxiety (trait and social) and depression. Results Females appear more sensitive to the romantic partner's consumption than males. For both sexes, having more friends who use cannabis appears to be a determinant. Depression and anxiety were not related to changes in cannabis use. Impulsivity is a significant factor for the maintenance of cannabis use in emerging adulthood, with higher lack of premeditation for males and higher sensation seeking for females among fluctuating and persistent users. Conclusions Results are discussed in terms of maintenance of use and the spiral of consumption, including clinical implications for prevention and interventions. [less ▲]Detailed reference viewed: 30 (6 ULg) Ethique d'un médecin de familleJamoulle, Marc Book published by Care Editions (2017)Préface Un médecin généraliste à l'endroit. Marc Jamoulle n'aime pas la médecine à l'envers, celle des étiquettes, de la maladie définie parce qu'elle répond aux trouvailles des pharmas, celle qui ... [more ▼]Préface Un médecin généraliste à l'endroit. Marc Jamoulle n'aime pas la médecine à l'envers, celle des étiquettes, de la maladie définie parce qu'elle répond aux trouvailles des pharmas, celle qui distribue plus de produits que de soins, celle qui réduit l'humain en chiffres, ni celle des corps morcelés, des pièces détachées du garage hospitalier. La médecine à l'endroit accepte l'humain tel qu'il est et s'adapte au problème. Elle ne nie pas le vécu et ne juge pas. Faite de tendresse pour la personne, pour l'individu particulier et de compassion, elle n'est ni le gardien de la morale sociale, ni la courroie de transmission des grands principes. Marc Jamoulle n'est pas sur l'estrade des Académiciens, il reste près du poêle au fond de la classe et devient un "virtuose du complexe et du coq à l'âne". Il se fâche lorsque les outils qu'on lui offre ne sont pas adaptés à la réalité. L'informatisation de l'humain lui pose problème puisqu'on ne peut pas faire de la "télémétrie de la souffrance". Et que valent les données recueillies si personne ne les lit? Il rêve du carnet d'écolier, un objet transitionnel comme trace de l'agir et du respect d'une équipe. Les rêves de Marc Jamoulle peuvent aboutir à des chartes élaborées avec ses collègues, comme la charte pour une éthique de l'information. Toujours Marc construit son cheminement sur la réalité de son quotidien professionnel: il nous raconte des histoires de patients, décrit ses journées, ses pensées, ses préoccupations et celles de ses confrères et progressivement il crée ses concepts ancrés dans la vie: les Q-Codes, la prévention quaternaire. Sa vision est critique, consciente des dérives possibles induites par les pouvoirs, ce que l'on voit tout au long de sa carrière, de la déclaration de Cos à celle de Rio. Il a certainement des colères constructives et le livre s'ouvre et se termine par elles: la condamnation de la méthadone par les autorités privant les patients d'un traitement qui s'avérait protecteur de la personne, et plus récemment la différence des législations sur l'aide en fin de vie, obligeant certains patients à passer d'un pays à l'autre, à un moment de leur vie qui mériterait plus de respect. Le livre de Marc Jamoulle est un patch-work, un aller et retour entre l'expérience quotidienne et les prises de position politique, une pensée sauvage toujours en alerte du risque de récupération: l'industrie n'a-t-elle pas réussi à s'emparer de l'EBM? En effet les meilleures idées peuvent être finalement pasteurisées comme le dit la déclaration de Rio et Marc tient à conserver le goût et les saveurs de la vie. Daniel Widmer Médecin généraliste, Vice-président de l'Union Européenne de Médecine Omnipraticienne UEMO. [less ▲]Detailed reference viewed: 43 (0 ULg) Baudelaire: traductor auctoritasMogollon Zapata, Juan Manuel in Literatura: Historia, Teoría, Crítica (2017), 19(2), 19-51In order to achieve visibility in the media and a position recognized by both the public and their peers, translators are compelled to take advantage of spaces of enunciation such as those provided by ... [more ▼]In order to achieve visibility in the media and a position recognized by both the public and their peers, translators are compelled to take advantage of spaces of enunciation such as those provided by prefaces, criticism, or biographical notes. Thanks to these spaces, in which translators deploy discursive and institutional strategies that allow them to position themselves and their translation project, translators acquire the status of translator-auctoritas, that is, a level of symbolic authority capable of endowing them with a public image. Through the detailed analysis of the editorial strategies and institutional calculations implemented by Baudelaire in order to position his project of translating Edgar Allan Poe, we show how the poet achieves the status of translator-autoritas and the role the latter played in the construction of his own literary identity [less ▲]Detailed reference viewed: 117 (12 ULg) Réalisation du attelle de Thomas modifiée chez le bovinMaquet, Celine ; Casalta, Hélène ; Sartelet, Arnaud et alin Point Vétérinaire (2017), 377Detailed reference viewed: 16 (1 ULg) Réalisation du attelle de Thomas modifiée pour un membre antérieurMaquet, Celine ; Casalta, Hélène ; Touati, Kamal et alin Point Vétérinaire (2017), 377Detailed reference viewed: 12 (1 ULg) Réalisation du attelle de Thomas modifiée pour un membre postérieurMaquet, Celine ; Casalta, Hélène ; Touati, Kamal et alin Point Vétérinaire (2017), 377Detailed reference viewed: 12 (0 ULg)