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See detailZonula occludens-1/NF-κB/CXCL8: a new regulatory axis for tumor angiogenesis.
Lesage, Julien; Suarez-Carmona, Meggy; Neyrinck-Leglantier, Deborah et al

in FASEB Journal (2017), 31(4), 1678-1688

Zonula occludens-1 (ZO-1) is a submembrane scaffolding protein that may display proinvasive functions when it relocates from tight junctions into the cytonuclear compartment. This article examines the ... [more ▼]

Zonula occludens-1 (ZO-1) is a submembrane scaffolding protein that may display proinvasive functions when it relocates from tight junctions into the cytonuclear compartment. This article examines the functional involvement of ZO-1 in CXCL8/IL-8 chemokine expression in lung and breast tumor cells. ZO-1 small interfering RNA and cDNA transfection experiments emphasized regulation of CXCL8/IL-8 expression via a cytonuclear pool of ZO-1. Luciferase reporter assays highlighted a 173-bp region of CXCL8/IL-8 promoter that responded to ZO-1. Moreover, by using mutated promoter constructs, we identified a NF-κB site as critical in this activation. Furthermore, NF-κB pathway signaling analysis revealed both IκBα and p65 phosphorylation in ZO-1-overexpressing cells, and subsequent p65 silencing validated its requirement for CXCL8/IL-8 induction. Investigation of the functional implication of this regulatory axis next showed the proangiogenic activity of ZO-1 in both ex vivo and in vivo angiogenesis assays. Finally, we found that non-small-cell lung carcinoma that presented a cytonuclear ZO-1 pattern was significantly more angiogenic that that without detectable cytonuclear ZO-1 expression. Taken together, our results demonstrate that ZO-1 regulates CXCL8/IL-8 expression via the NF-κB signaling pathway and its p65 subunit, which subsequently modulates the transcription of IL-8. We also provide evidence of a newly identified regulatory pathway that could promote angiogenesis. Thus, our results support the concept that the ZO-1 shuttle from the cell junction to the cytonuclear compartment may affect both the intrinsic invasive properties of tumor cells and the establishment of the protumoral microenvironment. [less ▲]

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See detailUn contrat d'enseignement de la médecine du IIIe siècle avant notre ère : P.Heid. III 226
Ricciardetto, Antonio ULg

in Ricciardetto, Antonio; Marganne, Marie-Hélène (Eds.) En marge du Serment hippocratique : contrats et serments dans le monde gréco-romain. Actes de la Journée d'étude internationale (Université de Liège, 29 octobre 2014) (2017, April)

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See detailIntroduction
Ricciardetto, Antonio ULg

in Ricciardetto, Antonio; Marganne, Marie-Hélène (Eds.) En marge du Serment hippocratique : contrats et serments dans le monde gréco-romain. Actes de la Journée d'étude internationale (Université de Liège, 29 octobre 2014) (2017, April)

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See detailEntre Rome et l’Égypte romaine. Pour une étude de la nourrice entre littérature médicale et contrats de travail
Ricciardetto, Antonio ULg; Gourevitch, Danielle

in Ricciardetto, Antonio; Marganne, Marie-Hélène (Eds.) En marge du Serment hippocratique : contrats et serments dans le monde gréco-romain. Actes de la Journée d'étude internationale (Université de Liège, 29 octobre 2014) (2017, April)

Detailed reference viewed: 38 (4 ULg)
See detailGlobaLex / Update : Research Guide to Belgian Law
Malliet, Christoph; Desseilles, François ULg

Learning material (2017)

1. The Belgian Legal System in a Nutshell 2. Developments in Belgian Legal Documentation 3. Legal Documentation

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See detailOn the identification of paedomorphic and overwintering larval newts based on cloacal shape: review and guidelines
Denoël, Mathieu ULg

in Current Zoology (2017), 63(2), 165-173

Paedomorphosis is an alternative process to metamorphosis in which adults retain larval traits at the adult stage. It is frequent in newts and salamanders, where larvae reach sexual maturity without ... [more ▼]

Paedomorphosis is an alternative process to metamorphosis in which adults retain larval traits at the adult stage. It is frequent in newts and salamanders, where larvae reach sexual maturity without losing their gills. However, in some populations, larvae overwinter in water, while remaining immature. These alternative ontogenetic processes are of particular interest in various research fields, but have different causes and consequences, as only paedomorphosis allows metamorphosis to be bypassed before maturity. It is thus relevant to efficiently identify paedomorphs versus overwintering larvae. In this context, the aim of this paper was threefold: firstly, to perform a meta-analysis of the identification procedures carried out in the literature; secondly, to determine the effectiveness of body size to make inferences about adulthood by surveying natural newt populations of Lissotriton helveticus and Ichthyosaura alpestris, and thirdly, to propose easy guidelines for an accurate distinction between large larvae and paedomorphs based on an external sexual trait, which is essential for reproduction — the cloaca. More than half of the studies in the literature do not mention the diagnostic criteria used for determining adulthood. The criteria mentioned were the presence of mature gonads (10%), eggs laid (4%), courtship behaviour (10%), and external morphological sexual traits (39%) including the cloaca (24%). Body-size thresholds should not be used as a proxy for paedomorphosis, because overwintering larvae can reach a larger size than paedomorphs within the same populations. In contrast, diagnosis based on cloacal external morphology is recommended, as it can be processed by the rapid visual assessment of all caught specimens, thus providing straightforward data at the individual level for both sexes. [less ▲]

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See detailThe ‘male escape hypothesis’: sex-biased metamorphosis in response to climatic drivers in a facultatively paedomorphic amphibian
Mathiron, Anthony; Lena, Jean-Paul; Baouch, Sarah et al

in Proceedings of the Royal Society B : Biological Sciences (2017), 284(1853), 20170176

Paedomorphosis is a major evolutionary process that bypasses metamorphosis and allows reproduction in larvae. In newts and salamanders, it can be facultative with paedomorphs retaining gills and ... [more ▼]

Paedomorphosis is a major evolutionary process that bypasses metamorphosis and allows reproduction in larvae. In newts and salamanders, it can be facultative with paedomorphs retaining gills and metamorphs dispersing. The evolution of these developmental processes is thought to have been driven by the costs and benefits of inhabiting aquatic versus terrestrial habitats. In this context, we aimed at testing the hypothesis that climatic drivers affect phenotypic transition and the difference across sexes because sex-ratio is biased in natural populations. Through a replicated laboratory experiment, we showed that paedomorphic palmate newts (Lissotriton helveticus) metamorphosed at a higher frequency when water availability decreased and metamorphosed earlier when temperature increased in these conditions. All responses were sex-biased, and males were more prone to change phenotype than females. Our work shows how climatic variables can affect facultative paedomorphosis and support theoretical models predicting life on land instead of in water. Moreover, because males metamorphose and leave water more often and earlier than females, these results, for the first time, give an experimental explanation for the rarity of male paedomorphosis (the ‘male escape hypothesis’) and suggest the importance of sex in the evolution of paedomorphosis versus metamorphosis. [less ▲]

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See detailRésultats des enquêtes "Attrait des Sciences" (années académiques 2015–2016 et 2016–2017)
Aerts, Stéphanie ULg; Ernst, Marie ULg

Report (2017)

Lors des rentrées académiques 2015-2016 et 2016-2017, une enquête a été envoyée aux étudiants primants inscrits en Faculté des Sciences. Cette enquête interroge les étudiants sur leur parcours secondaire ... [more ▼]

Lors des rentrées académiques 2015-2016 et 2016-2017, une enquête a été envoyée aux étudiants primants inscrits en Faculté des Sciences. Cette enquête interroge les étudiants sur leur parcours secondaire, leur choix d'étude, leur avenir professionnel et leurs aptitudes. Ce rapport consiste en une analyse descriptive et exploratoire des résultats des deux enquêtes. [less ▲]

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See detailThe shadow of Montaigne in the political works of Gabriel Naudé
Decoster, Sara ULg

Conference (2017, March 31)

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See detailLes Q-Codes et la terminologie multilingue de médecine générale et de famille
Jamoulle, Marc ULg; Schuers, Matthieu; Ouvrard, Patrick et al

Conference (2017, March 31)

voir pdf

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See detailStaatssteun aan groene energie
Van Cleynenbreugel, Pieter ULg

Scientific conference (2017, March 31)

In this presentation, I tackle the subject of aid in the context of green energy, discussing the legal framework as well as cases and pitfalls.

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See detailProposals for a further expansion of day surgery in Belgium
Leroy, Roos; Camberlin, Cécile; Lefevre, Mélanie ULg et al

Report (2017)

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See detailDFT investigation of the reaction mechanism for the guanidine catalyzed ring-opening of cyclic carbonates by aromatic and alkyl-amines
Alves, Margot ULg; Méreau, Raphaël; Grignard, Bruno ULg et al

in RSC Advances (2017), 7(31), 18993-19001

The guanidine catalysed aminolysis of propylene carbonate has been investigated using the density functional theory (DFT) and highlights that different reaction pathways are involved depending on the ... [more ▼]

The guanidine catalysed aminolysis of propylene carbonate has been investigated using the density functional theory (DFT) and highlights that different reaction pathways are involved depending on the aromatic or aliphatic nature of the amine. The structural ability of 1,5,7-triazabicyclo[4.4.0]dec-5-ene (TBD) to simultaneously give and receive protons was demonstrated by a detailed mechanistic investigation. The bifunctional activity (base/H-bond donor) of TBD significantly reduces the Gibbs energy of the reaction and allows understanding its higher efficiency compared to its methyl counterpart (MTBD). [less ▲]

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See detailImportance of monitoring parenteral nutrition
SENTERRE, Thibault ULg

in International Conference for Advancing Nutrition – Neonatology, Brussels (2017, March 30)

Detailed reference viewed: 13 (1 ULg)