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See detailIl ne suffit pas de lire les étiquettes, mais bien de discuter avec le producteur
Ozer, Pierre ULg

Article for general public (2014)

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See detailRational Design of a Safe and Efficacious Attenuated Recombinant Vaccine Against Cyprinid Herpesvirus 3 Using Prokaryotic Mutagenesis and In Vivo Imaging System
Boutier, Maxime ULg

Conference (2014, October 02)

Cyprinid herpesvirus 3 (CyHV-3) is the causative agent of a lethal disease in common and koi carp. Since its emergence, in the late 1990s, CyHV 3 has caused severe economic losses worldwide creating a ... [more ▼]

Cyprinid herpesvirus 3 (CyHV-3) is the causative agent of a lethal disease in common and koi carp. Since its emergence, in the late 1990s, CyHV 3 has caused severe economic losses worldwide creating a need for a safe and efficacious vaccine. With this goal in mind, recombinant strains deleted for single gene were produced using prokaryotic recombination technologies. While producing such a recombinant for ORF134, we unexpectedly obtained a clone deleted for ORF56 and ORF57 in addition to the expected deletion of ORF134. Interestingly, this triple deleted recombinant replicated efficiently in vitro, exhibited an attenuated profile and induced an immune protection against a lethal challenge in vivo. To determine the role of each deleted locus in the phenotype of the triple deleted recombinant, a series of recombinant were produced and tested in vivo. Firstly, to investigate the role of ORF134 deletion, a double ORF56-57 deleted recombinant and an independent triple ORF56-57-ORF134 deleted recombinant were produced. These experiments revealed that ORF134 deletion did contribute significantly neither to the attenuation nor to the immune protection observed for the triple deleted recombinant. Secondly, to determine the relative importance of ORF56 and ORF57 deletion in the attenuation observed, two single deleted recombinants were produced for ORF56 and ORF57. These experiments demonstrated that ORF57 deletion, in contrast to ORF56 deletion, correlates with an attenuated phenotype. Based on its safety-efficacy profile, the ORF56-57 double deleted recombinant was selected as a candidate vaccine. Complementary experiments were performed to further investigate the potential of this strain as a safe and efficacious attenuated recombinant vaccine. The results obtained can be summarized as follows: (i) In vivo imaging (IVIS) of vaccinated fish challenged with a wild type strain expressing luciferase as a reporter gene, suggested that vaccination induces a sterile immunity at the portal of entry. (ii) Study of viral tropism by qPCR, IVIS, and histopathological analyses, demonstrated that in comparison to the wild type parental strain, the vaccine strain replicates at lower level, at later time post-infection and for a shorter period of time. (iii) Transmission studies demonstrated that there is no detectable spread of the vaccine from freshly vaccinated fish to naive fish located immediately down stream of vaccinated fish (water sharing). All together, the results of the present study demonstrate the potential of ORF56-ORF57 double deleted CyHV-3 recombinant strain for safe and efficacious mass vaccination of carp. [less ▲]

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See detailSimulation and optimization of a CHP biomass plant and district heating network
Sartor, Kevin ULg; Quoilin, Sylvain ULg; Dewallef, Pierre ULg

in Applied Energy (2014), 130

Biomass Combined Heat and Power (CHP) plants connected to district heating (DH) networks are recognized nowadays as a very good opportunity to increase the share of renewable sources into energy systems ... [more ▼]

Biomass Combined Heat and Power (CHP) plants connected to district heating (DH) networks are recognized nowadays as a very good opportunity to increase the share of renewable sources into energy systems. However, as CHP plants are not optimized for electricity production, their operation is profitable only if a sufficient heat demand is available throughout the year. Most of the time, pre-feasibility studies are based on peak power demand and business plans only assume monthly or yearly consumption data. This approach usually turns out to overestimate the number of operating hours or oversize the plant capacity. This contribution presents a methodology intended to be simple and effective that provides accurate estimations of economical, environmental and energetic performances of CHP plants connected to district heating networks. A quasi-steady state simulation model of a CHP plant combined with a simulation model of the district heating network installed on the Campus of the University in Liège (Belgium) is used as an application framework to demonstrate the effectiveness of the selected approach. Based on the developed model and actual consumption data, several scenarios for energy savings are considered and ranked. The potential energy savings and resulting energy costs are estimated enabling more general conclusions to be drawn on the opportunity of using district heating networks in urban districts for Western Europe countries. [less ▲]

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See detailÉTUDE DU COMPLEXE ENZYMATIQUE DES SYMBIONTES DU TERMITE RETICULITERMES FLAVIPES (EX. SANTONENSIS)
Tarayre, Cédric ULg

Doctoral thesis (2014)

In the light of the economic, environmental and social context in which we live today, it has become obvious that the use of fossil fuels is not conceivable over the long term. Some alternatives have ... [more ▼]

In the light of the economic, environmental and social context in which we live today, it has become obvious that the use of fossil fuels is not conceivable over the long term. Some alternatives have therefore emerged in recent years. Second-generation biofuels are one of those alternatives and are based on the exploitation of vegetal biomass, also called lignocellulosic biomass. These materials require a hydrolysis step which can notably be achieved by enzymes. Some insects, such as termites, harbor complex microbial communities inside their digestive tracts. Those communities are able to produce enzymes which can be used in the field of the hydrolysis of vegetal biomass. This is what this thesis deals with. The main part of the work done focused on the research of enzyme-producing microorganisms (bacteria, mycetes and protists) responsible for the degradation of hemicelluloses and cellulose, the hydrolysis of which releases fermentable sugars. The insect which was used in this work was Reticulitermes flavipes (ex. santonensis), a lower termite, harboring a highly diversified internal microflora. This thesis describes the characterization of the microbial strains which were isolated and the enzymes they secrete. A complementary part of this research focussed on termites grown on artificial diets. The objective of this part was multiple : isolating enzyme-producing strains, not extractable according to the standard technique, and characterizing the microflora resulting from the applied artificial diets. This multidisciplinary approach was based on microscopy, proteomics, metagenomics and the assessment of metabolism applied to the different microbial consortia. [less ▲]

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See detailMicrocrystalline cellulose, a direct compression binder in a quality by design environment—A review
Thoorens, Grégory; Krier, Fabrice ULg; Leclercq, Bruno et al

in International Journal of Pharmaceutics (2014), 473(1-2), 64-72

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See detailLiège. Où s’amorce le nouveau modèle économique wallon ? Où s’échoue le vieux modèle politique wallon ?
Artige, Lionel ULg

Conference given outside the academic context (2014)

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See detailLes châteaux industriels dans le bassin liégeois : typologie et étude de cas
Destatte, Julien ULg; Peters, Arnaud ULg; Van Velthem, Lionel

Conference (2014, October 01)

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See detailViolences dans les relations sentimentales et orientations au niveau de l'intervention
Glowacz, Fabienne ULg

Conference given outside the academic context (2014)

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See detailVelocity and turbulence measurements for assessing the stability of riverbeds: a comparison between UVP and ADVP
Duma, Diana ULg; Erpicum, Sébastien ULg; Archambeau, Pierre ULg et al

in 11th International Conference on Hydroscience & Engineering - "Hydro-Engineering for Environmental Challenges" (2014, October)

Maintaining the overall stability of active riverbeds requires a deep understanding of the complex interactions between turbulent flow forces and forces stabilizing the riverbed. Standard approaches do ... [more ▼]

Maintaining the overall stability of active riverbeds requires a deep understanding of the complex interactions between turbulent flow forces and forces stabilizing the riverbed. Standard approaches do not properly take into account the turbulence fluctuations in non-uniform flows, while these are of primary importance in the mechanisms determining the stability of stones. In a new approach, the flow forces are quantified by means of parameters combining explicitly the velocity and turbulence distributions over a certain water depth above the riverbed. In this paper, as an onset for using these newly developed bed stability parameters, we report on new experimental measurements of velocity and turbulent kinetic energy based on acoustic methods, whereas all previous uses at the new bed stability parameters relied on Laser PIV Techniques. For quasi-uniform and non-uniform flow conditions, velocity measurements were conducted with two complementary devices: an ultrasonic velocimeter probe (UVP) and an acoustic Doppler velocimeter profiler (ADVP). The results are compared and discussed in detail. A general consistency between the two types of measurements is obtained, while some discrepancies are highlighted close to the bed and tentative explanations are given. [less ▲]

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See detailPlasma riche en plaquettes et lésions tendineuses
KAUX, Jean-François ULg; Drion, Pierre ULg; Croisier, Jean-Louis ULg et al

in Revue Médicale de Liège (2014), 69(Synthèse 2014), 67-72

Platelets contain growth factors released during their degranulation following activation. These growth factors promote tissue remodeling, wound healing and angiogenesis. Currently, the clinical effect of ... [more ▼]

Platelets contain growth factors released during their degranulation following activation. These growth factors promote tissue remodeling, wound healing and angiogenesis. Currently, the clinical effect of Platelet-Rich Plasma (PRP) is still discussed or even controversial. Our researches have evaluated the effectiveness of PRP on the healing of animal tendons and human suffering from chronic jumper's knee. [less ▲]

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See detailInvestigation of shear stress evolution during neotissue growth in a perfusion bioreactor using 3d multiphysics modeling
Guyot, Yann ULg; Papantoniou, Ioannis; Schrooten, Jan et al

Conference (2014, October)

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See detailAn asymptotic expansion-based method for a spectral approach in equivalent statistical linearization
Canor, Thomas ULg; Blaise, Nicolas ULg; Denoël, Vincent ULg

in Probabilistic Engineering Mechanics (2014), 38

Equivalent linearization consists in replacing a nonlinear system with an equivalent linear one whose parameters are tuned with regard to the minimization of a suitable function. In particular, the ... [more ▼]

Equivalent linearization consists in replacing a nonlinear system with an equivalent linear one whose parameters are tuned with regard to the minimization of a suitable function. In particular, the Gaussian equivalent linearization expresses the properties of an equivalent linear system in terms of the mean vector and the covariance matrix of the responses, which are the unknowns of the optimization problem in a spectral approach. Even though the system has been linearized, the resulting set of equations is nonlinear. The computational effort in this method pertains to the solution of a possibly large set of nonlinear algebraic equations involving integrals and inversions of full matrices. This work proposes to develop and apply an asymptotic expansion-based method to facilitate and to improve the statistical linearization for large nonlinear structures. The proposed developments demonstrate that for slightly to moderately coupled nonlinear systems, the equivalent linearization can be applied with an appropriate modal approach and eventually seen as a convergent series initiated with the stochastic response of a main decoupled linear system. With this method, the computational effort is attractively reduced, the conditioning of the set of nonlinear algebraic equations is improved and inversion of full transfer matrices and repeated integrations are avoided. The paper gives a formal description of the method and illustrates its implementation and performances with the computation of stationary responses of nonlinear structures subject to coherent random excitation fields. [less ▲]

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See detailRetrospective analysis of a suburban out-ofhours clinic in Belgium
Belche, Jean-Luc ULg; Berrewaerts, Marie-Astrid ULg; Burette, Philippe ULg et al

in Acta Clinica Belgica (2014), 69(5), 341-347

Introduction: In many countries, out-of-hours medical care is under scrutiny. The aim of this article is to study the activities recorded by the first out-of-hours clinic that has been opened, as a pilot ... [more ▼]

Introduction: In many countries, out-of-hours medical care is under scrutiny. The aim of this article is to study the activities recorded by the first out-of-hours clinic that has been opened, as a pilot study, in two Walloon communes. Material and method: A retrospective analysis of anonymized data was conducted for 2009. Coding of diagnoses was conducted using the International Classification of Primary Care (ICPC-2). Results: A total of 3949 contacts were recorded in 2009 with the out-of-hours clinic, 3294 related to inhabitants of the two communes covered, which was equivalent to 13% of the total population in question. Compared to 7.2% of contacts between midnight and 8 a.m., 82.9% of contacts took place between 8 a.m. and 9 p.m., and 91.6% of contacts were handled locally, with only 8.4% resulting in hospitalization. In addition, 52% of contacts were with patients aged between 25 and 65; 29.9% of contacts were with paediatric patients (,15 years). Patients over the age of 65 made up 18% of contacts. The most common pathologies were respiratory (R). Analysis of flu diagnoses identified two epidemic peaks. Discussion: The suburban out-of-hours clinic studied fulfilled an important role in managing the demand for health care. The large majority of health problems were resolved locally, and the inhabitants did not need to go to hospital. Appointments between midnight and 8 a.m. were in the minority, which points towards adjusting the organization of the out-of-hours service during the night. The geriatric population is not highly over-represented contrary to what might be expected considering its largest number of pathologies. The on-call doctor’s skills profile should take account of the populations and morbidities encountered. Out-ofhours clinics could possibly play a sentinel role in terms of flu epidemics. Conclusion: This study describes a pilot suburban out-of-hours clinic which met three of recommendations set by the KCE in its report on out-of-hours care in general medicine: the organization of an out-of-hours clinic with logistical support, the use of a single telephone number and merging out-of-hours areas. While debate exists on the management of out-of-hours care, this study provides evidence on the role of the physician during these hours. [less ▲]

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See detailNumerical investigation of cut-edge effect using the Gurson-Tvergaard-Needleman model
Lemoine, Xavier; Tudor, Balan; Habraken, Anne ULg

in Procedia Engineering (2014, October)

In this contribution, the formability of sheet metal cut edges is investigated using damage model.Classical forming limit diagrams are known not to apply properly to the cut edges. However, with mild ... [more ▼]

In this contribution, the formability of sheet metal cut edges is investigated using damage model.Classical forming limit diagrams are known not to apply properly to the cut edges. However, with mild steels the sheet edges usually behave better than the Forming Limit Diagram prédictions, so this phenomenon has not been given sufficient attention. In contrast, for Advanced High Strengh Steels the cut edges exhibit reduced formability as compared to the plain sheet;this effect is very sensitive to the quality of the cutting process. The current investigation is aimed to evaluate the ability of available damage models to predict this effect on sample applications. The Gurson-Tvergaard-Needleman model is used and the material parameters for a Dual Phase are considered [1]. The effect of the cutting process is described by means of initial fields of equivalent plastic strain and porosity. The geometrical distribution and typical values for these two initial fields are devised based on literature . Numerical simulations of flat notched tensile tests are used within the FE code Abaqus/Explicit to illustrate the impact of the initial fields on the moment and the location of the failure initiation. The influence of the mesh size in the cutting-affected area is also investigated. The hole expansion test and a flat bending test are further simulated to investigate the influence of the cut edge. [less ▲]

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See detailLes valeurs et les valorisations des images
Dondero, Maria Giulia ULg

in Biglari, Amir (Ed.) Les valeurs. Aux fondements de la sémiotique (2014)

Cet article vise à explorer la manière dont la sémiotique greimassienne a traité la question des valeurs dans le cadre de l’image, notamment photographique et artistique. Après une relecture critique de ... [more ▼]

Cet article vise à explorer la manière dont la sémiotique greimassienne a traité la question des valeurs dans le cadre de l’image, notamment photographique et artistique. Après une relecture critique de l’ouvrage de Jean-Marie Floch, Les formes de l’empreinte, et de la distinction entre valeurs dans l’image et valorisations des images, ce travail se consacre à l’exposition de nouvelles propositions de la sémiotique des pratiques de Jacques Fontanille en essayant de les lier à des réflexions sur les objets et les pratiques de la photographie artistique (l’utilisation des séries, de l’original, des différents tirages, etc.). Si l’attention est portée surtout sur la photo artistique, ce texte envisage également une comparaison avec les pratiques de l’original et de l’authenticité dans la photo de guerre, la photo de famille et la photo-image pieuse. L’objectif majeur de ce travail est d’étudier les diverses pratiques de valorisation des images photographiques afin de faire éclater la notion ontologique de trace photographique. [less ▲]

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See detailThe impact and retention of spray droplets on a horizontal hydrophobic surface
Zwertvaegher, Ingrid; Verhaeghe, Micheline; Brusselman, Eva et al

in Biosystems Engineering (2014), 126

Spray retention, i.e. the overall capture of spray droplets by plants on initial or subsequent impact, and after loss due to run-off, is an important stage in the spray application process as droplet ... [more ▼]

Spray retention, i.e. the overall capture of spray droplets by plants on initial or subsequent impact, and after loss due to run-off, is an important stage in the spray application process as droplet losses may result in reduced efficacy, economic loss, and environmental contamination. The aim of this exploratory study is to determine whether a new method based on calculating the volumetric proportions per impact type, i.e. adhesion, rebound and shatter, can be used to predict spray retention. These volumetric proportions are calculated based on logistic regression models, derived from vision-based droplet characteristics and impact assessments, and laser-based spray characteristics. The advantages and limitations of such a method are explored. The volumetric proportions per impact type on a horizontal, synthetic hydrophobic surface were determined for four different nozzles (XR 110 01 VS flat-fan nozzle, XR 110 04 VS flat-fan nozzle, XR 110 08 VS flat-fan nozzle and AI 110 08 VS air-induction nozzle) under controlled realistic conditions, and compared to the results of a retention test. The volumetric proportions of adhesion were much lower than the relative retentions, indicating that a considerable amount of rebound and shatter also contributed to final retention. The method should thus be improved by including the droplets retained after first impact and the retained proportions of partial droplet fragmentation but it is nevertheless considered a promising technique. [less ▲]

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See detailAzione, testualizzazione, notazione
Dondero, Maria Giulia ULg

in Espressione e contenuto. Rivista on-line dell'AISS Associazione Italiana di Studi Semiotici (2014, October)

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