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See detailLa réforme des soins de santé mentale en Belgique: pas complètement convergents, mais concrètement interdépendants
Thunus, Sophie ULg

Conference (2015, January 08)

Dans cet article, nous retracerons d’abord les grandes lignes des évolutions des associations d’usagers et de proches en santé mentale, en Europe et en Belgique. Ensuite, nous présenterons une ... [more ▼]

Dans cet article, nous retracerons d’abord les grandes lignes des évolutions des associations d’usagers et de proches en santé mentale, en Europe et en Belgique. Ensuite, nous présenterons une expérimentation politique dans le champ de la santé mentale en Belgique, qui a été l’occasion, pour les associations d’usagers et de proches, d’institutionnaliser leur participation. A cette occasion, nous évoquerons les motifs politiques à l’origine du projet de participation (acte 1) confié par les autorités publiques belges aux associations, ses modalités, et son implémentation au sein d’un dispositif d’action publique impliquant également des experts professionnels et scientifiques. Dans un troisième temps, nous nous intéresserons à la poursuite du projet participation dans le cadre de la réforme actuelle de la santé mentale et de la psychiatrie. Nous décrirons les modalités d’implémentation du projet (acte 2) et les nouveaux défis qui se posent aux associations, au fur-et-à-mesure de l’augmentation et de la visibilité accrue de leur participation aux niveaux institutionnels et politiques. En conclusion, nous soulignerons le caractère contingent de l’émergence et de la stabilisation de la participation en Belgique. Nous verrons que ce caractère contingent est à l’origine de fortes interdépendances entre le développement de la participation, des projets politiques spécifiques et trajectoires individuelles particulières. Cette interdépendance génère à son tour des enjeux spécifiques, pour les associations et les représentants des usagers, en termes identitaires et de légitimité. Nous soulignerons en outre le caractère incrémental du processus par lequel les associations stabilisent leur discours, leur méthodologie et leur intervention concrète dans la prise de décision aux niveaux macro et micro. A cet égard, mettrons en évidence l’importance de la production continue de documents (recommandations, questionnaires, …) stabilisant les éléments-clé de ce discours et permettant aux experts par le vécu de le porter en différents endroits, au sein des institutions des soins et dans la sphère politique. Finalement, nous envisagerons l’hypothèse de la professionnalisation des représentant(e)s des associations et des représentants des usagers, tout en soulignant les enjeux personnels et structurels associés à cette professionnalisation. [less ▲]

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See detailAutour du sujet défait
Vrancken, Didier ULg

Conference (2015, January 07)

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See detailRecent summer Arctic atmospheric circulation anomalies in a historical perspective
Belleflamme, Alexandre ULg; Fettweis, Xavier ULg; Erpicum, Michel ULg

in Cryosphere (The) (2015), 9

A significant increase in the summertime occurrence of a high pressure area over the Beaufort Sea, the Canadian Arctic Archipelago, and Greenland has been observed since the beginning of the 2000s, and ... [more ▼]

A significant increase in the summertime occurrence of a high pressure area over the Beaufort Sea, the Canadian Arctic Archipelago, and Greenland has been observed since the beginning of the 2000s, and particularly between 2007 and 2012. These circulation anomalies are likely partly responsible for the enhanced Greenland ice sheet melt as well as the Arctic sea ice loss observed since 2007. Therefore, it is interesting to analyse whether similar conditions might have happened since the late 19th century over the Arctic region. We have used an atmospheric circulation type classification based on daily mean sea level pressure and 500 hPa geopotential height data from five reanalysis data sets (ERA-Interim, ERA-40, NCEP/NCAR, ERA-20C, and 20CRv2) to put the recent circulation anomalies in perspective with the atmospheric circulation variability since 1871. We found that circulation conditions similar to 2007–2012 have occurred in the past, despite a higher uncertainty of the reconstructed circulation before 1940. For example, only ERA-20C shows circulation anomalies that could explain the 1920–1930 summertime Greenland warming, in contrast to 20CRv2. While the recent anomalies exceed by a factor of 2 the interannual variability of the atmospheric circulation of the Arctic region, their origin (natural variability or global warming) remains debatable. [less ▲]

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See detailLa période hollandaise en Belgique
Brüll, Christoph ULg

Conference given outside the academic context (2015)

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See detailHigh-density mapping of the MHC identifies a shared role for HLA-DRB1*01:03 in inflammatory bowel diseases and heterozygous advantage in ulcerative colitis.
Goyette, Philippe; Boucher, Gabrielle; Mallon, D et al

in Nature Genetics (2015)

Genome-wide association studies of the related chronic inflammatory bowel diseases (IBD) known as Crohn's disease and ulcerative colitis have shown strong evidence of association to the major ... [more ▼]

Genome-wide association studies of the related chronic inflammatory bowel diseases (IBD) known as Crohn's disease and ulcerative colitis have shown strong evidence of association to the major histocompatibility complex (MHC). This region encodes a large number of immunological candidates, including the antigen-presenting classical human leukocyte antigen (HLA) molecules. Studies in IBD have indicated that multiple independent associations exist at HLA and non-HLA genes, but they have lacked the statistical power to define the architecture of association and causal alleles. To address this, we performed high-density SNP typing of the MHC in >32,000 individuals with IBD, implicating multiple HLA alleles, with a primary role for HLA-DRB1*01:03 in both Crohn's disease and ulcerative colitis. Noteworthy differences were observed between these diseases, including a predominant role for class II HLA variants and heterozygous advantage observed in ulcerative colitis, suggesting an important role of the adaptive immune response in the colonic environment in the pathogenesis of IBD. [less ▲]

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See detailIchtyofaune du bassin du fleuve Mono (Bénin et Togo): diversité, écologie et impacts anthropiques
Lederoun, Djiman ULg

Doctoral thesis (2015)

This study has three main objectives: (1) to obtain an inventory of the ichthyofauna of the Mono River that is as complete as possible; (2) to study the ecology of this ichthyofauna; and (3) to document ... [more ▼]

This study has three main objectives: (1) to obtain an inventory of the ichthyofauna of the Mono River that is as complete as possible; (2) to study the ecology of this ichthyofauna; and (3) to document the impact of fishing on the exploited stocks based on two targeted species. The inventory was made by compiling the literature, by studying collections of museums and of other institutions and by several sampling campaigns, conducted between February 2011 and March 2014. This resulted in an inventory of 91 fish species belonging to 67 genera, 42 families and 14 orders. The Perciformes (31 species), Siluriformes (18), Osteoglossiformes (10), Cypriniformes (9) and Characiformes (9) are the most diverse families in the Mono River Basin. The most species-rich families are the Cichlidae (9 espèces), Cyprinidae (9), Mormyridae (7), Clariidae (7) and Alestidae (6). Twenty-nine species, of which 7 of freshwater and 22 of marine or estuarine origin have been added to the ichtyofauna as known before this study. Nine species present in museum collections and three species only known from the literature have not been collected again. Several taxonomic problems have been encountered. Two of these cases were studied in depth. Foremost, the taxonomic status of Brycinus leuciscus and B. luteus (Alestiidae, Characiformes) was revised based on specimens originating from the complete distribution range of the two nominal species. This allowed us to synonymise the two species, with Brycinus leuciscus as senior synonym. The latter has been erroneously mentioned from the Mono River. Our results, however, confirmed the presence of two phenotypes, i.e. one with and one without a pre-dorsal hump, which was mentioned in the literature as intra-specific variation in B. leuciscus. A comparative study comparing specimens belonging to 'Barbus' nigeriensis (Cyprinidae, Cypriniformes) of the Mono Basin with those originating from the basins of the Volta, the Ogun et the Niger revealed the presence of a new species, described as Barbus "Vandewalle", a Volta Basin endemic. An indentification key of the different families, genera and species from the Mono Basin is presented. For the ecological study, two seasonal sampling campaigns were performed (from December to mid-March and from mid-July to mid-September) in 2012 and 2013 using gill nets of different mesh-size (10, 12, 17, 20 et 22 mm knot to knot). Sampling was performed at 10 sampling sites that were located upstream and downstream of the Adjarala Falls (the site of a planned hydroelectric plant, 100 km upstream of that of Nangbéto) and upstream and downstream of the dam at Nangbéto. Seventeen physico-chemical and environmental variables were taken at these sites. The ordination of the sampling sites in relation to their values for the physico-chemical, environmental variables and to their fish assemblages was studied using a non-linear method: the self organising map (SOM). The samples were scattered in four groups in function of their physico-chemical and environmental variables. The distance to the source, height of the canopy, rocks, coverage of the canopy, altitude, gravel, mud, sand, water velocity and dissolved oxygen were the most discriminating factors for the obtained groups. The zone of the Adjarala, where a new dam will be constructed, differs by its rocky substrate, the velocity of the water and the relatively high amount of dissolved oxygen. For the ichthyofauna, the SOM suggest a structuring in three groups. Our results oppose the tendency to homogeny in the middle course of rivers, which is probably due to the presence of the dam at Nangbéto. A significant correlation was found between the distribution of the species and the physico-chemical and environmental parameters. The distance to the source, altitude, forested river banks, height of the canopy, velocity of the water, conductivity, transparency and pH were the parameters that explained the distribution of the fish community best. The level of disturbance of the ecosystem, which was studied using the Abundance Biomass Comparison (ABC) showed that the zones at and near the dam at Nangbéto were the most stressed. A length-weight and length-length key was designed for the first time for the 37 species that were the most abundant in the catches. This was done in order to improve the quality of the fisheries statistics collected by fisheries officers on the scale of the basin. The analysis of the allometric coefficient b of the length-weight relation showed that the majority of the fishes had an allometric growth. The condition factor calculated in order to estimate the amount of flesh on the fish showed that the environment was heavily disturbed. To conclude, from the results of the study on the impact of the fisheries on targeted species, it became clear that the population of Sarotherodon melanotheron melanotheron is over-exploited at Lake Toho. At Lakes Doukon and Togbadji, on the other hand, an under-exploitation of the population of S. galilaeus galilaeus was observed. The latter illustrates the possibility of sustainable management even in the presence of intensive fishing pressure. As, in this thesis, several cases of anthropogenic impacts were identified, a regular surveillance programme will be paramount in order to preserve the ichthyological diversity of the Mono River Basin. [less ▲]

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See detailEffect of stress path on the miniaturizatio size effect for nickel polycrystals
Keller, Clement; Hug, Eric; Duchene, Laurent ULg et al

Conference (2015, January 04)

The mechanical behavior of mettalic materials deeply depends on the size of samples. For specimen dimensions de creasing from a few millimeters to a few micrometers, the general observed trend is a ... [more ▼]

The mechanical behavior of mettalic materials deeply depends on the size of samples. For specimen dimensions de creasing from a few millimeters to a few micrometers, the general observed trend is a softening of the mechanical behavior in tension which affects the stress level and the strain hardening. This effect is triggered by the derease of the number of grains across the thickness ( also called thickness"t" over grain size "d" ratio). The objective of this work is to provide new experimental results in order to analyse the miniaturization size effects for various stress paths without strain gradients across the thickness of the samples. To this aim, experimental tensil tests, large tensile tests and shear tests have been performed on Ni sheets ( 0.5mm) with various grain sizes ensuring different t/d ratios. Results show that the miniaturization softening is affected by triaxiality, the larger is this parameter, the lower is the mechanicval softening. These features seem to be linked to surface effects which are larger for low triaxiality stress paths. Attempts of numerical simulations using strain gradient crystal plasticity model are also performed to confirm the role played by surface effects. [less ▲]

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See detailUpscaling of an electronic nose for continuously stirred tank reactor stability monitoring from pilot-scale to real-scale agricultural co-digestion biogas plant
Adam, Gilles ULg; Lemaigre, Sébastien; Goux, Xavier et al

in Bioresource Technology (2015), 178

This study investigated the use of an electronic nose for on-line anaerobic reactor state monitoring at the pilot-scale level and then upscaling to the full-scale level. E-nose indicator was compared to ... [more ▼]

This study investigated the use of an electronic nose for on-line anaerobic reactor state monitoring at the pilot-scale level and then upscaling to the full-scale level. E-nose indicator was compared to classical state indicators such as pH, alkalinity, volatile fatty acids concentration and to other gas phase compounds. Multivariate statistical process control method, based on principal component analysis and the Hotelling's T² statistics was used to derive an indicator representative of the reactor state. At the pilot-scale level, the e-nose indicator was relevant and could distinguish 3 process states: steady-state, transient and collapsing process. At the full-scale level, the e-nose indicator could provide the warning of the major disturbance whereas two slight disturbances were not detected and it gave one major false alarm. This work showed that gas phase relation with anaerobic process should be deeper investigated, as an e-nose could indicate the reactor state, focusing on the gas phase. [less ▲]

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See detailBasin-scale partitioning of Greenland ice sheet mass balance components (2007–2011)
Andersen, M.L.; Stenseng, L.; Skourup, H. et al

in Earth and Planetary Science Letters (2015), 409

The current deficit in Greenland ice sheet mass balance is due to both a decrease in surface mass balance (SMB ) input and an increase in ice discharge (D ) output. While SMB processes are beginning to be ... [more ▼]

The current deficit in Greenland ice sheet mass balance is due to both a decrease in surface mass balance (SMB ) input and an increase in ice discharge (D ) output. While SMB processes are beginning to be well captured by observationally-constrained climate modeling, insight into D is relatively limited. We use InSAR-derived velocities, in combination with ice thickness observations, to quantify the mass flux (F ) across a flux perimeter around the ice sheet at ∼1700 m elevation. To quantify D , we correct F for SMB , as well as changes in volume due to ice dynamics, in the area downstream of the gate. Using a 1961–1990 reference climatology SMB field from the MAR regional climate model, we quantify ice sheet mass balance within eighteen basins. We find a 2007–2011 mean D of View the MathML source. We find a 2007–2011 mean total mass balance of View the MathML source, which is equal to a 0.73 mm yr−1 global sea level rise contribution. This mass loss is dominated by SMB, which accounts for 61% of mass loss in the basins where partitioning is possible. [less ▲]

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See detailV444 Cygni X-ray and polarimetric variability: Radiative and Coriolis forces shape the wind collision region
Lomax, J. R.; Nazé, Yaël ULg; Hoffman, J. L. et al

in Astronomy and Astrophysics (2015), 573

We present results from a study of the eclipsing, colliding-wind binary V444 Cyg that uses a combination of X-ray and optical spectropolarimetric methods to describe the 3D nature of the shock and wind ... [more ▼]

We present results from a study of the eclipsing, colliding-wind binary V444 Cyg that uses a combination of X-ray and optical spectropolarimetric methods to describe the 3D nature of the shock and wind structure within the system. We have created the most complete X-ray light curve of V444 Cyg to date using 40 ks of new data from Swift, and 200 ks of new and archived XMM-Newton observations. In addition, we have characterized the intrinsic, polarimetric phase-dependent behavior of the strongest optical emission lines using data obtained with the University of Wisconsin's Half-Wave Spectropolarimeter. We have detected evidence of the Coriolis distortion of the wind-wind collision in the X-ray regime, which manifests itself through asymmetric behavior around the eclipses in the system's X-ray light curves. The large opening angle of the X-ray emitting region, as well as its location (i.e. the WN wind does not collide with the O star, but rather its wind) are evidence of radiative braking/inhibition occurring within the system. Additionally, the polarimetric results show evidence of the cavity the wind-wind collision region carves out of the Wolf-Rayet star's wind. [less ▲]

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See detailThe Gaia-ESO Survey: CNO abundances in the open clusters Trumpler 20, NGC 4815, and NGC 6705
Tautvaišienė, G.; Drazdauskas, A.; Mikolaitis, Š. et al

in Astronomy and Astrophysics (2015), 573

Context. The Gaia-ESO Public Spectroscopic Survey will observe a large sample of clusters and cluster stars, covering a wide age-distance-metallicity-position-density parameter space. <BR /> Aims: We aim ... [more ▼]

Context. The Gaia-ESO Public Spectroscopic Survey will observe a large sample of clusters and cluster stars, covering a wide age-distance-metallicity-position-density parameter space. <BR /> Aims: We aim to determine C, N, and O abundances in stars of Galactic open clusters of the Gaia-ESO survey and to compare the observed abundances with those predicted by current stellar and Galactic evolution models. In this pilot paper, we investigate the first three intermediate-age open clusters. <BR /> Methods: High-resolution spectra, observed with the FLAMES-UVES spectrograph on the ESO VLT, were analysed using a differential model atmosphere method. Abundances of carbon were derived using the C[SUB]2[/SUB] band heads at 5135 and 5635.5 Å. The wavelength interval 6470-6490 Å, with CN features, was analysed to determine nitrogen abundances. Oxygen abundances were determined from the [O i] line at 6300 Å. <BR /> Results: The mean values of the elemental abundances in Trumpler 20 as determined from 42 stars are: [ Fe/H ] = 0.10 ± 0.08 (s.d.), [ C/H ] = -0.10 ± 0.07, [ N/H ] = 0.50 ± 0.07, and consequently C/N = 0.98 ± 0.12. We measure from five giants in NGC 4815: [ Fe/H ] = -0.01 ± 0.04, [ C/H ] = -0.17 ± 0.08, [ N/H ] = 0.53 ± 0.07, [ O/H ] = 0.12 ± 0.09, and C/N = 0.79 ± 0.08. We obtain from 27 giants in NGC 6705: [ Fe/H ] = 0.0 ± 0.05, [ C/H ] = -0.08 ± 0.06, [ N/H ] = 0.61 ± 0.07, [ O/H ] = 0.13 ± 0.05, and C/N = 0.83 ± 0.19. The C/N ratios of stars in the investigated open clusters were compared with the ratios predicted by stellar evolutionary models. For the corresponding stellar turn-off masses from 1.9 to 3.3 M[SUB]⊙[/SUB], the observed C/N ratio values are very close to the predictions of standard first dredge-up models as well as to models of thermohaline extra-mixing. They are not decreased as much as predicted by the recent model in which the thermohaline- and rotation-induced extra-mixing act together. The average [O/H] abundance ratios of NGC 4815 and NGC 6705 are compared with the predictions of two Galactic chemical evolution models. The data are consistent with the evolution at the solar radius within the errors. <BR /> Conclusions: The first results of CNO determinations in open clusters show the potential of the Gaia-ESO Survey to judge stellar and Galactic chemical evolution models and the validity of their physical assumptions through a homogeneous and detailed spectral analysis. Based on data products from observations made with ESO Telescopes at the La Silla Paranal Observatory under programme ID 188.B-3002 (The Gaia-ESO Public Spectroscopic Survey, PIs G. Gilmore and S. Randich). [less ▲]

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See detailFeature issue of digital holography and 3D imaging (DH): introduction
Hayasaki, Yoshio; Liu, Jung-Ping; Georges, Marc ULg

in Applied Optics (2015), 54(1), 1-2

The OSA Topical Meeting “Digital Holography and 3D Imaging (DH)” was held in Seattle, Washington, 13–17 July 2014. Feature issues based on the DH meeting series have been released by Applied Optics (AO ... [more ▼]

The OSA Topical Meeting “Digital Holography and 3D Imaging (DH)” was held in Seattle, Washington, 13–17 July 2014. Feature issues based on the DH meeting series have been released by Applied Optics (AO) since 2007. In this year (2014), Optics Express (OE) and AO jointly decided to have one such feature issue in each journal. The feature issue includes 27 papers and covers a large range of topics, reflecting the rapidly expanding techniques and applications of digital holography and 3D imaging. The DH meeting will continue in the future, as expected, and the next meeting is scheduled to be held on24–28 May 2015, at Shanghai Institute of Optics and Fine Mechanics, Shanghai, China. [less ▲]

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See detailFood provioning influences ranging patterns in northern pigtailed macaques (Macaca leonina)
Huynen, Marie-Claude ULg; Savini, Tommaso ULg; Asensio, Norberto et al

in International Journal of Primatology (2015, January)

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See detailDocumentário e mal-entendido. Retorno sobre uma primeira filmagem no Brasil
Hamers, Jérémy ULg

in Stols, Eddy; Mascaro, Luciana; Bueno, Clodoaldo (Eds.) Brasil e Bélgica: Cinco Séculos de Conexões e Interação (2015)

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See detailPrivate Versus Official? New Hypotheses for the Reconstruction of Bronze Age Upper Mesopotamian Economic System
Mas, Juliette ULg

Conference (2015, January)

Ancient economic systems from Southern and Central Mesopotamia are quite well known when Northern Mesopotamia ones remain less known. If we already know that, during Bronze Age, households played a ... [more ▼]

Ancient economic systems from Southern and Central Mesopotamia are quite well known when Northern Mesopotamia ones remain less known. If we already know that, during Bronze Age, households played a deciding role in several kinds of production, their degree of dependence or independence to the State still has to be clarified . The archaeological data clearly show that private workshops, set up in private houses, could be very efficient and specialized. Furthermore, these economic entities could be assimilated to private enterprises, which were able to handle their own administrative duties. On the other hand, we know from the texts that the Palace and the temples were also a main element of ancient economic systems. If public administrations should, in some cases, have supplied raw materials, hired workforce, and dealt with the distribution of produced goods, it seems that the production didn’t take place in the palace itself. Dealing both with archaeological and textual data could help to better define the role and the importance of both public and private components in early Mesopotamian economic systems. [less ▲]

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See detailDiversité des expressions culturelles: retour du politique
Vlassis, Antonios ULg

Report (2015)

Cette édition spéciale aborde les enjeux actuels de la mise en œuvre de la Convention sur la diversité des expressions culturelles (CDEC) à travers une analyse des questions discutées lors de la 8ème ... [more ▼]

Cette édition spéciale aborde les enjeux actuels de la mise en œuvre de la Convention sur la diversité des expressions culturelles (CDEC) à travers une analyse des questions discutées lors de la 8ème session du Comité intergouvernemental de la CDEC. À cet égard, nous revenons dans un premier temps sur l’impact normatif de la CDEC et sur la question des rapports périodiques des Parties à la CDEC. Dans un deuxième temps, nous traitons du débat autour de la mise en œuvre du Fonds international pour la diversité culturelle et des articles 16 (Traitement préférentiel) et 21 (Concertation et coordination internationales), ainsi que de la question de l’adaptation de la CDEC aux défis des technologies numériques. Les interventions des participants lors de la session du Comité et le caractère du débat nous révèlent le retour du politique dans la CDEC, à savoir que l’étape actuelle de sa mise en œuvre implique nécessairement des divergences et des conflits politiques, vu l’importance économique, sociale et culturelle des questions traitées. Un tableau récapitulatif des principaux événements qui ont marqué 2014 vient compléter ce tour d’horizon. [less ▲]

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See detailN1-Fluoroalkyltryptophan analogues: synthesis and in vitro study as potential substrates for indoleamine 2,3-dioxygenase
Henrottin, Jean ULg; Zervosen, Astrid ULg; Lemaire, Christian ULg et al

in ACS Medicinal Chemistry Letters (2015)

ABSTRACT: Indoleamine 2,3-dioxygenase (hIDO) is an enzyme that catalyzes the oxidative cleavage of the indole ring of L-tryptophan through the kynurenine pathway, thereby exerting immunosuppressive ... [more ▼]

ABSTRACT: Indoleamine 2,3-dioxygenase (hIDO) is an enzyme that catalyzes the oxidative cleavage of the indole ring of L-tryptophan through the kynurenine pathway, thereby exerting immunosuppressive properties in inflammatory and tumoral tissues. The syntheses of 1-(2-fluoroethyl)-tryptophan (1-FETrp) and 1-((1-(2-fluoroethyl)-1H-1,2,3-triazol-4-yl)methyl)-tryptophan, two N1-fluoroalkylated tryptophan derivatives, are described here. In vitro enzymatic assays with these two new potential substrates of hIDO show that 1-FETrp is a good and specific substrate of hIDO. Therefore, its radioactive isotopomer, 1-[18F]FETrp, should be a molecule of choice to visualize tumoral and inflammatory tissues and/or to validate new potential inhibitors. [less ▲]

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See detailMetabolic reprogramming in transformed mouse cortical astrocytes: a proteomic study.
Bentaib, Azeddine; De Tullio, Pascal ULg; Chneiweiss, Herve et al

in Journal of proteomics (2015), 113

Metabolic reprogramming is thought to play a key role in sustaining the survival and proliferation of cancer cells. These changes facilitate for example the uptake and release of nutrients required for ... [more ▼]

Metabolic reprogramming is thought to play a key role in sustaining the survival and proliferation of cancer cells. These changes facilitate for example the uptake and release of nutrients required for nucleotide, protein and lipid synthesis necessary for macromolecule assembly and tumor growth. We applied a 2D-DIGE (Two-Dimensional Differential in-Gel Electrophoresis) quantitative proteomic analysis to characterize the proteomes of mouse astrocytes that underwent in vitro cancerous transformation, and of their normal counterparts. Metabolic reprogramming effects on enzymatic and structural protein expression as well as associated metabolites abundance were quantified. Using enzymatic activity measurements and zymography, we documented and confirmed several changes in abundance and activity of various isoenzymes likely to participate in metabolic reprogramming. We found that after transformation, the cells increase their expression of glycolytic enzymes, thus augmenting their ability to use aerobic glycolysis (Warburg effect). An increased capacity to dispose of reducing equivalents through lactate production was also documented. Major effects on carbohydrates, amino acids and nucleotides metabolic enzymes were also observed. Conversely, the transformed cells reduced their enzymatic capacity for reactions of tricarboxylic acid oxidation, for neurotransmitter (glutamate) metabolism, for oxidative stress defense and their expression of astroglial markers. BIOLOGICAL SIGNIFICANCE: The use of a global approach based on a 2D DIGE analysis allows obtaining a comprehensive view of the metabolic reprogramming undergone by astrocytes upon cancerous transformation. Indeed, except for a few enzymes such as pyruvate carboxylase and glutaminase that were not detected in our initial analysis, pertinent information on the abundance of most enzymes belonging to pathways relevant to metabolic reprogramming was directly obtained. In this in vitro model, transformation causes major losses of astrocyte-specific proteins and functions and the acquisition of metabolic adaptations that favor intermediate metabolites production for increased macromolecule biosynthesis. Thus our approach appears to be readily applicable for the investigation of changes in protein abundance that determine various transformed cell phenotypes. It could similarly be applied to the evaluation of the effects of treatments aimed at correcting the consequences of cell transformation. [less ▲]

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See detailChine, une mise à jour
Florence, Eric ULg

in Politique : Revue de Débats (2015), 88

Au lendemain de la répression du mouvement démocratique de 1989, journalistes, analystes et autres « China watchers » abondaient en commentaires crépusculaires sur la pérennité du régime chinois ... [more ▼]

Au lendemain de la répression du mouvement démocratique de 1989, journalistes, analystes et autres « China watchers » abondaient en commentaires crépusculaires sur la pérennité du régime chinois. L’argument le plus souvent évoqué était celui d’un décalage insoutenable entre réformes économiques – qui connaîtront un approfondissement à l’occasion du fameux « voyage dans le Sud » de Deng Xiaoping en 1992 – et l’absence de réformes politiques, un décalage qui devait presque immanquablement signifier la fin du régime communiste. Quelque vingt-cinq ans plus tard, où en est-on ? [less ▲]

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