Deletion of cysteine cathepsins B or L yields differential impacts on murine skin proteome and degradome

in Molecular & Cellular Proteomics (2013), 12(3), 611-25

Numerous studies highlight that concerted proteolysis is essential for skin morphology and function. The cysteine protease cathepsin L (Ctsl) has been implicated in epidermal proliferation and ... [more ▼]

Numerous studies highlight that concerted proteolysis is essential for skin morphology and function. The cysteine protease cathepsin L (Ctsl) has been implicated in epidermal proliferation and desquamation as well as in hair cycle regulation. In stark contrast, mice deficient for cathepsin B (Ctsb) do not display an overt skin phenotype. To understand the systematic consequences of deleting Ctsb or Ctsl, we determined protein abundances of > 1300 proteins and proteolytic cleavage events in skin samples of wild-type, Ctsb-/- and Ctsl-/- mice by mass spectrometry based proteomics. Both protease deficiencies revealed distinct quantitative changes in proteome composition. Ctsl-/- skin revealed increased levels of the cysteine protease inhibitors cystatin B and cystatin M/E, increased cathepsin D and accumulation of the extracellular glycoprotein periostin. Immunohistochemistry located periostin predominantly in hypodermal connective tissue of Ctsl-/- skin. Proteomic identification of proteolytic cleavage sites within skin proteins revealed numerous processing sites that are underrepresented in Ctsl-/- or Ctsb-/- samples. Notably, few of the affected cleavage sites shared the canonical Ctsl or Ctsb specificity, providing further evidence for a complex proteolytic network in the skin. Novel processing sites in proteins such as dermokine and Notch-1 were detected. Simultaneous analysis of acetylated protein N-termini showed prototypical mammalian N-alpha acetylation. These results illustrate an influence of both Ctsb and Ctsl on the murine skin proteome and degradome with the phenotypic consequences of the absence of either protease differing considerably. [less ▲]

Investigation of the Determinants of Travelers’ Mental Knowledge of Public Parking Facilities

in Proceedings of the 92nd Annual Meeting of the Transportation Research Board (DVD-ROM) (2013)

This paper describes a study of car drivers’ familiarity with the parking situation in the vicinity of a regional shopping center. The data used for this study are collected in Hasselt, a medium sized ... [more ▼]

This paper describes a study of car drivers’ familiarity with the parking situation in the vicinity of a regional shopping center. The data used for this study are collected in Hasselt, a medium sized city in Belgium. The central shopping area of Hasselt is surrounded by 23 public parking facilities. 1007 residents have been asked to indicate if they are familiar with each parking facility. The concept of familiarity was related to the socio-demographic and cognitive attributes of the respondents, their trips to the city center and the type of parking facility using multinomial logistic regression and bivariate probit regression. The results show that familiarity with parking facilities is especially related to age and education, and to the frequency of car use towards the city centre, and to a lesser extent to place of residence, income and perceived mental knowledge. In addition to these results, this paper demonstrates the value of collecting virtual buffer data by means of an online survey tool. The authors recommend that simulation models that predict parking choice behavior take into account the different levels of familiarity with parking facilities, and the contributing factors. An important avenue for further research is the combination of personal and facility specific information to assess the familiarity with different types of parking facilities. [less ▲]

Decision making for teeth replacement in the esthetic area

Scientific conference (2013)

Esthetic results in dentistry are determined by the harmony between the dental prostheses and the soft tissues. To reach an optimal result, each single step of the treatment has a great importance, from ... [more ▼]

Esthetic results in dentistry are determined by the harmony between the dental prostheses and the soft tissues. To reach an optimal result, each single step of the treatment has a great importance, from the smile analyses to the final restoration delivery. The goal of this lecture is to describe how to elaborate a treatment planning for single and multiple tooth replacement in the aesthetic zone. A decision tree for the surgical protocols will be proposed according to our clinical experience at the university of Liège and, the current scientific evidences. The overall objective is, from an initial clinical situation, to determine the appropriate treatment approach and timing. The following scientific aspects will be developed: - The biological concept of extraction socket preservation. The use of biomaterials for alveolar bone augmentation or socket preservation. The goal is to clarify properties of the mostly used biomaterial in dentistry, as well as determine their clinical indications and limitations. Soft tissue management around teeth and implant [less ▲]

Validation of a French Version of a New Anxiety Trait Scale for Children

Poster (2013)

Anxiety diagnosis is relatively complex in children because intensity as well as symptoms of anxiety change during childhood (Bouden, Halayem, & Fakhfakh, 2002). The principal aim of this study was to ... [more ▼]

Anxiety diagnosis is relatively complex in children because intensity as well as symptoms of anxiety change during childhood (Bouden, Halayem, & Fakhfakh, 2002). The principal aim of this study was to validate through Confirmatory Factor Analyses (CFA) the a priori three-factor structure of the French version of the anxiety trait scale for children which includes psychological (“tend to be worried about everything”), behavioral (“tend to be upset, nervous or grumbling”), and somatic symptoms (“headache complaints”) of anxiety. This scale was previously found to discriminate, with high sensitivity and specificity, children with anxiety from control group. A first CFA performed on 288 6-12 year-old children showed an acceptable fit (2/df =2.66; RMSEA=.07 and CFI=.94). A second three-factor model was constructed and showed a better fit with a new sample of 287 children (2/df =2.18; RMSEA=.06 and CFI=.96), with a lower ECVI value for the model 2. For this model, the Cronbach’s alpha for each of the subscales ranged from .71 to .86, which confirmed the good internal reliability of the scale. This study provides a new three-factor structure for this anxiety scale and proposes normative data for French-speaking children. [less ▲]

Internet Topology Discovery

in Biersack, Ernst; Callegari, Christian; Matijasevic, Maja (Eds.) Data Traffic Monitoring and Analysis: From Measurement, Classification, and Anomaly Detection to Quality of Experience (2013)

Since the nineties, the Internet has seen an impressive growth, in terms of users, intermediate systems (such as routers), autonomous systems, or applications. In parallel to this growth, the research ... [more ▼]

Since the nineties, the Internet has seen an impressive growth, in terms of users, intermediate systems (such as routers), autonomous systems, or applications. In parallel to this growth, the research community has been looking for obtaining and modeling the Internet topology, i.e., how the various elements of the network interconnect between themselves. An impressive amount of work has been done regarding how to collect data and how to analyse and model it. This chapter reviews main approaches for gathering Internet topology data. We first focus on hop limited probing, i.e., traceroute-like probing. We review large-scale tracerouting projects and discuss traceroute limitations and how they are mitigated by new techniques or extensions. Hop limited probing can reveal an IP interface vision of the Internet. We next focus on techniques for aggregating several IP interfaces of a given router into a single identifier. This leads to a router level vision of the topology. The aggregation can be done through a process called alias resolution. We also review a technique based on IGMP probing that silently collect all multicast interfaces of a router into a single probe. We next refine the router level topology by adding subnet information. We finish this chapter by discussing the AS level topology, in particular the relationships between ASes and the induced hierarchy. [less ▲]

Préface

in Nespoulet, Roland; Chiotti, Laurent; Henry-Gambier, Dominique (Eds.) Le Gravettien final de l'abri Pataud (Dordogne, France). Fouilles et études 2005-2009 (2013)

Effect of exercise on innate immunity in horses

in Proceedings of the 6th Congress of the European College of Equine Internal Medicine (ECEIM): ACVIM, Le Touquet France (2013)

Comment étudier la communication entre dauphins captifs et soigneurs ?

in Grandgeorge, Marine; Le Pevedic, Brigitte; Pugniere, Frédéric (Eds.) Interactions et Intercompréhension : une approche comparative (2013)

Cet article présente la manière dont nous avons abordé la communication interspécifique dans le cas d’une étude toujours en cours sur les systèmes de communication formés par des soigneurs et leurs ... [more ▼]

Cet article présente la manière dont nous avons abordé la communication interspécifique dans le cas d’une étude toujours en cours sur les systèmes de communication formés par des soigneurs et leurs dauphins au Parc Astérix de Paris. La première partie aborde les principales difficultés théoriques et méthodologiques qui se sont posées à nous pour la description de ce système de communication hétérogène. La seconde partie présente le cadre théorique mobilisé, les choix méthodologiques et les résultats attendus (les analyses sont toujours en cours). La troisième partie pose la question de l’intercompréhension sous la forme d’une compréhension sociale qui n’exclut pas le malentendu. [less ▲]

Méthodes archéologiques

Book published by De Boeck (2013)

Iconicité démonstrative. Dispositifs producteurs de l’évidence géométrique d’une preuve formelle

in Visible (2013)