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See detailCombination of Partial Least Squares regression and Design of Experiments to model the retention of pharmaceuticals in Supercritical Fluid Chromatography
Andri, Bertyl ULg; Dispas, Amandine ULg; Marini Djang'Eing'A, Roland ULg et al

in Journal of Chromatography. A (2017)

This work presents a first attempt to establish a model of the retention behaviour for pharmaceutical compounds in gradient mode SFC. For this purpose, multivariate statistics were applied on the basis of ... [more ▼]

This work presents a first attempt to establish a model of the retention behaviour for pharmaceutical compounds in gradient mode SFC. For this purpose, multivariate statistics were applied on the basis of data gathered with the Design of Experiment (DoE) methodology. It permitted to build optimally the experiments needed, and served as a basis for providing relevant physicochemical interpretation of the effects observed. Data gathered over a broad experimental domain enabled the establishment of well-fit linear models of the retention of the individual compounds in presence of methanol as co-solvent. These models also allowed the appreciation of the impact of each experimental parameter and their factorial combinations. This approach was carried out with two organic modifiers (i.e. methanol and ethanol) and provided comparable results. Therefore, it demonstrates the feasibility to model retention in gradient mode SFC for individual compounds as a function of the experimental conditions. This approach also permitted to highlight the predominant effect of some parameters (e.g. gradient slope and pressure) on the retention of compounds. Because building of individual models of retention was possible, the next step considered the estab- lishment of a global model of the retention to predict the behaviour of given compounds on the basis of, on the one side, the physicochemical descriptors of the compounds (e.g. Linear Solvation Energy Relationship (LSER) descriptors) and, on the other side, of the experimental conditions. This global model was established by means of partial least squares regression for the selected compounds, in an experimental domain defined by the Design of Experiment (DoE) methodology. Assessment of the model’s predic- tive capabilities revealed satisfactory agreement between predicted and actual retention (i.e. R2 = 0.942, slope = 1.004) of the assessed compounds, which is unprecedented in the field. [less ▲]

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See detailA Large and Consistent Phylogenomic Dataset Supports Sponges as the Sister Group to All Other Animals.
Simion, Paul; Philippe, Herve; Baurain, Denis ULg et al

in Current Biology (2017)

Resolving the early diversification of animal lineages has proven difficult, even using genome-scale datasets. Several phylogenomic studies have supported the classical scenario in which sponges (Porifera ... [more ▼]

Resolving the early diversification of animal lineages has proven difficult, even using genome-scale datasets. Several phylogenomic studies have supported the classical scenario in which sponges (Porifera) are the sister group to all other animals ("Porifera-sister" hypothesis), consistent with a single origin of the gut, nerve cells, and muscle cells in the stem lineage of eumetazoans (bilaterians + ctenophores + cnidarians). In contrast, several other studies have recovered an alternative topology in which ctenophores are the sister group to all other animals (including sponges). The "Ctenophora-sister" hypothesis implies that eumetazoan-specific traits, such as neurons and muscle cells, either evolved once along the metazoan stem lineage and were then lost in sponges and placozoans or evolved at least twice independently in Ctenophora and in Cnidaria + Bilateria. Here, we report on our reconstruction of deep metazoan relationships using a 1,719-gene dataset with dense taxonomic sampling of non-bilaterian animals that was assembled using a semi-automated procedure, designed to reduce known error sources. Our dataset outperforms previous metazoan gene superalignments in terms of data quality and quantity. Analyses with a best-fitting site-heterogeneous evolutionary model provide strong statistical support for placing sponges as the sister-group to all other metazoans, with ctenophores emerging as the second-earliest branching animal lineage. Only those methodological settings that exacerbated long-branch attraction artifacts yielded Ctenophora-sister. These results show that methodological issues must be carefully addressed to tackle difficult phylogenetic questions and pave the road to a better understanding of how fundamental features of animal body plans have emerged. [less ▲]

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See detailANTICANCER, ANTIPLASMODIAL AND ANTITRYPANOSOMAL ACTIVITIES OF CRUDE EXTRACTS OF PLATANUS ORIENTALIS
Ebralidze, L.; Mskhiladze, Lasha; Ledoux, Allison ULg et al

in World Journal of Pharmaceutical Research (2017), 6(3), 170-175

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See detailDeciding game invariance
Duchêne, Eric; Parreau, Aline; Rigo, Michel ULg

in Information & Computation (2017), 253

In a preivous paper, Duchêne and Rigo introduced the notion of invariance for take-away games on heaps. Roughly speaking, these are games whose rulesets do not depend on the position. Given a sequence S ... [more ▼]

In a preivous paper, Duchêne and Rigo introduced the notion of invariance for take-away games on heaps. Roughly speaking, these are games whose rulesets do not depend on the position. Given a sequence S of positive tuples of integers, the question of whether there exists an invariant game having S as set of P -positions is relevant. In particular, it was recently proved by Larsson et al. that if S is a pair of complementary Beatty sequences, then the answer to this question is always positive. In this paper, we show that for a fairly large set of sequences (expressed by infinite words), the answer to this question is decidable. [less ▲]

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See detailComparison of emergence times and quality between isoflurane and sevoflurane in rhesus macaque (Macaca mulatta) undergoing neurosurgical procedure
Bertrand, Henri ULg; Springer, Svenja; Burnside, Wesley et al

in Laboratory Animals (2017)

Volatile agents for anaesthesia are widely used for anaesthetizing laboratory primates, and isoflurane is one of the most frequently used agents. Sevoflurane has been shown to offer a more rapid recovery ... [more ▼]

Volatile agents for anaesthesia are widely used for anaesthetizing laboratory primates, and isoflurane is one of the most frequently used agents. Sevoflurane has been shown to offer a more rapid recovery than iso- flurane in a number of species, but no comparisons have been made in non-human primates. This study compared the recovery characteristics of isoflurane and sevoflurane in rhesus macaques undergoing experi- mental neurosurgery. Twelve primates (7 males and 5 females) were randomly allocated to the treatment groups. They were sedated with ketamine (10 mg/kg) and anaesthesia was induced with propofol (usually 8 mg/kg intravenously [IV]). Anaesthesia was maintained with either sevoflurane (SEVO) (2.2 0.4%) or iso- flurane (ISO) (1.2 0.2%) and alfentanil (0.2–0.5 mg/kg/min IV) for 332–592 min. Animals were mechanically ventilated. Meloxicam (0.3mg/kg) and methylprednisolone infusion (5.4mg/kg/h) were also administered. Time to extubation after cessation of anaesthesia was significantly shorter with sevoflurane (ISO: 7.0 1.8 min; SEVO: 3.6 1.5; *P 1⁄4 0.005) as was the time to the animal sitting unaided (ISO: 15.7 8.2 min; SEVO: 7.1 1.7min; *P1⁄40.004). No significant difference in the quality of recovery following isoflurane or sevoflurane anaesthesia was found. In conclusion, isoflurane and sevoflurane are both suitable volatile agents for the maintenance of general anaesthesia in rhesus macaques undergoing experimental neurosur- gical procedures. The two volatile agents presented a similar emergence quality profile, however sevoflurane anaesthesia was associated with a faster recovery, offering the possibility of conducting earlier post-operative neurological assessment. [less ▲]

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See detailThree Mamluk Letters concerning the Florentine Trade in Egypt and Syria. A new interpretation.
Rizzo, Alessandro ULg

in Mamlūk Cairo: A Crossroad for Embassies (2017)

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See detailDiplomatie sur le terrain : la première mission diplomatique florentine en territoire mamelouk.
Rizzo, Alessandro ULg

in Culture matérielle et contacts diplomatiques entre l’Occident latin, Byzance et l’Orient islamique (XIe-XVIe siècle). (2017)

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See detailRelations on words
Rigo, Michel ULg

in Indagationes Mathematicae (2017), 28

In the first part of this survey, we present classical notions arising in combinatorics on words: growth function of a language, complexity function of an infinite word, pattern avoidance, periodicity and ... [more ▼]

In the first part of this survey, we present classical notions arising in combinatorics on words: growth function of a language, complexity function of an infinite word, pattern avoidance, periodicity and uniform recurrence. Our presentation tries to set up a unified framework with respect to a given binary relation. In the second part, we mainly focus on abelian equivalence, $k$-abelian equivalence, combinatorial coefficients and associated relations, Parikh matrices and $M$-equivalence. In particular, some new refinements of abelian equivalence are introduced. [less ▲]

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See detailNanomedicine as a potent strategy in melanoma tumor microenvironment
Pautu, Vincent ULg; Leonetti, Daniela; Lepeltier, Elise et al

in Pharmacological Research (2017)

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See detailInfusion of mesenchymal stromal cells after deceased liver transplantation: A phase I-II, open-label, clinical study.
DETRY, Olivier ULg; VANDERMEULEN, Morgan ULg; DELBOUILLE, Marie-Hélène ULg et al

in Journal of Hepatology (2017)

BACKGROUND & AIM: Mesenchymal stromal cell (MSC) infusion could be a mean to establish tolerance in solid organ recipients. The aim of this prospective, controlled, phase-1 study was to evaluate the ... [more ▼]

BACKGROUND & AIM: Mesenchymal stromal cell (MSC) infusion could be a mean to establish tolerance in solid organ recipients. The aim of this prospective, controlled, phase-1 study was to evaluate the feasibility, safety and tolerability of a single infusion of MSCs in liver transplant recipients. METHODS: Ten liver transplant recipients under standard immunosuppression received 1.5-3x106/kg third-party unrelated MSCs on post-operative day 3+/-2, and were prospectively compared to a control group of 10 liver transplant recipients. As primary end-points, MSC infusional toxicity was evaluated, and infectious and cancerous complications were prospectively recorded until month 12 in both groups. As secondary end-points, rejection rate, month-6 graft biopsies, and peripheral blood lymphocyte phenotyping were compared. Progressive immunosuppression weaning was attempted from month 6 to 12 in MSC recipients. RESULTS: No variation in vital parameters or cytokine release syndrome could be detected during and after MSC infusion. No patient developed impairment of organ functions (including liver graft function) following MSC infusion. No increased rate of opportunistic infection or de novo cancer was detected. As secondary end-points, there was no difference in overall rates of rejection or graft survival. Month-6 biopsies did not demonstrate a difference between groups in the evaluation of rejection according to the Banff criteria, in the fibrosis score or in immunohistochemistry (including Tregs). No difference in peripheral blood lymphocyte typing could be detected. The immunosuppression weaning in MSC recipients was not successful. CONCLUSIONS: No side effect of MSC infusion at day 3 after liver transplant could be detected, but this infusion did not promote tolerance. This study opens the way for further MSC or Treg-based trials in liver transplant recipients. LAY SUMMARY: Therapy with mesenchymal stromal cells (MSCs) has been proposed as a mean to improve results of solid organ transplantation. One of the potential MSC role could be to induce tolerance after liver transplantation, i.e. allowing the cessation of several medications with severe side effects. This study is the first-in-man use of MSC therapy in 10 liver transplant recipients. This study did not show toxicity after a single MSC infusion but it was not sufficient to allow withdrawal of immunosuppression. [less ▲]

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See detailSoil organic carbon fractionation for improving agricultural soil quality assessment – a case study in Southern Belgium (Wallonia)
Trigalet, Sylvain; Chartin, Caroline; Krüger, Inken ULg et al

in Biotechnologie, Agronomie, Société et Environnement = Biotechnology, Agronomy, Society and Environment (2017), 21(S1),

Description of the subject. The paper presents and discusses a method for fractionating bulk soil organic carbon (SOC) in meaningful SOC fractions to better assess SOC status and its related soil ... [more ▼]

Description of the subject. The paper presents and discusses a method for fractionating bulk soil organic carbon (SOC) in meaningful SOC fractions to better assess SOC status and its related soil ecosystem functions. Objectives. The objective is to perform an evaluation of ecosystem functions of soil organic matter at plot scale and compare it to the normal operative range of the local agro-ecological region. Method. By separating carbon associated with clay and fine silt particles (stable carbon with slow turnover rate, < 20 μm) and carbon non-associated with this fraction (labile and intermediate carbon with higher turnover rates, ≥ 20 μm), effects of management can be detected more efficiently at different scales. Conclusions. Soil organic carbon fractions, used as proxies for soil ecosystem functions, can be helpful because they represent SOC functional pools. This paper proposes to apply fractionation on samples taken at plot and regional scale. It is therefore possible to establish a normal operative range for a specific agro-region for comparison with the values in individual plots. This allows drawing a baseline for SOC fractions status in a specific agricultural unit. This approach provides valuable information to study and evaluate the impact of agricultural management in the context of enhancing soil quality and functions. [less ▲]

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See detailIntegrating biological indicators in a Soil Monitoring Network (SMN) to improve soil quality diagnosis – a study case in Southern Belgium (Wallonia)
Krüger, Inken ULg; Chartin, Caroline; van Wesemael, Bas et al

in Biotechnologie, Agronomie, Société et Environnement = Biotechnology, Agronomy, Society and Environment (2017), 31(S1),

Soil organisms and their activities are essential for soil ecosystem functioning and they can thus be used as pertinent indicators of soil quality. Recent efforts have been undertaken to include ... [more ▼]

Soil organisms and their activities are essential for soil ecosystem functioning and they can thus be used as pertinent indicators of soil quality. Recent efforts have been undertaken to include biological indicators of soil quality into regional/national monitoring networks. Objectives. The aim of this study was to provide a first dataset of six biological indicators and two eco-physiological quotients for two landscape units in Wallonia. These spatial units are characterized by homogeneous climate conditions, soil type, land-use and management (here, grasslands in the Ardennes, and croplands in the Loam Region). Method. Respiration potential, microbial biomass carbon and nitrogen, net nitrogen mineralization, metabolic potential of soil bacteria and earthworm abundance were measured at a total of 60 sites in two different landscape units (LSU). Variability within each LSU was studied. Data was synthesized through calculation of a comprehensive score and presentation as radar plots. Results. All selected biological indicators were significantly higher under grassland than under cropland soils, highlighting the biological indicators’ power of discrimination between main land use types. Variability within LSU depended on the biological indicator and was generally higher in grassland than in cropland soils. Each site could unambiguously be assigned to its landscape unit based on its calculated comprehensive score. Radar plots allowed an assessment of the distribution of values within a landscape unit at a glance. Conclusions. The pilot-study defined the first baseline values for agricultural soils in Wallonia and laid the foundation for a monitoring network of biological soil quality. [less ▲]

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See detailComment j'utilise le blended learning et la classe inversée
Pochet, Bernard ULg

Speech/Talk (2017)

Make a little history Present the reasons for my choice Presenting the current framework Present the principles of the flipped classroom Show how I use the blended learning and flipped classroom Some ... [more ▼]

Make a little history Present the reasons for my choice Presenting the current framework Present the principles of the flipped classroom Show how I use the blended learning and flipped classroom Some questions ... [less ▲]

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See detailDisease Note. First Report of Persimmon Cryptic Virus in Spain
Ruiz-Garcia, A-B; Chamberland, N.; Martinez, C. et al

in Journal of Plant Pathology [=JPP] (2017), 99(1),

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See detailEekenhof
Leyh, Valérie ULg

in Philipp Theisohn; Christian Demandt (Eds.) Theodor Storm-Handbuch. Leben - Werk - Wirkung (2017)

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See detailIm Brauer-Hause
Leyh, Valérie ULg

in Philipp Theisohn; Christian Demandt (Eds.) Theodor Storm-Handbuch. Leben - Werk - Wirkung (2017)

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See detailThe Politics and Aesthetics of Hunger and Disgust: Perspectives on the Dark Grotesque
Delville, Michel ULg; Norris, Andrew

Book published by Routledge (2017)

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See detailWaldwinkel
Leyh, Valérie ULg

in Christian Demandt; Philipp Theisohn (Eds.) Theodor Storm-Handbuch. Leben - Werk - Wirkung (2017)

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