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See detailDiscovery and characterization of EIIB, a new α-conotoxin from Conus ermineus venom by nAChRs affinity capture monitored by MALDI-TOF/TOF mass spectrometry
Echterbille, Julien; Gilles, Nicolas; Araoz, Romulo et al

in Toxicon (2017), 130

Animal toxins are peptides that often bind with remarkable affinity and selectivity to membrane receptors such as nicotinic acetylcholine receptors (nAChRs). The latter are, for example, targeted by α ... [more ▼]

Animal toxins are peptides that often bind with remarkable affinity and selectivity to membrane receptors such as nicotinic acetylcholine receptors (nAChRs). The latter are, for example, targeted by α-conotoxins, a family of peptide toxins produced by venomous cone snails. nAChRs are implicated in numerous physiological processes explaining why the design of new pharmacological tools and the discovery of potential innovative drugs targeting these receptor channels appear so important. This work describes a methodology developed to discover new ligands of nAChRs from complex mixtures of peptides. The methodology was set up by the incubation of Torpedo marmorata electrocyte membranes rich in nAChRs with BSA tryptic digests (>100 peptides) doped by small amounts of known nAChRs ligands (α-conotoxins). Peptides that bind to the receptors were purified and analyzed by MALDI-TOF/TOF mass spectrometry which revealed an enrichment of α-conotoxins in membrane-containing fractions. This result exhibits the binding of α-conotoxins to nAChRs. Negative controls were performed to demonstrate the specificity of the binding. The usefulness and the power of the methodology were also investigated for a discovery issue. The workflow was then applied to the screening of Conus ermineus crude venom, aiming at characterizing new nAChRs ligands from this venom, which has not been extensively investigated to date. The methodology validated our experiments by allowing us to bind two α-conotoxins (α-EI and α-EIIA) which have already been described as nAChRs ligands. Moreover, a new conotoxin, never described to date, was also captured, identified and sequenced from this venom. Classical pharmacology tests by radioligand binding using a synthetic homologue of the toxin confirm the activity of the new peptide, called α-EIIB. The Ki value of this peptide for Torpedo nicotinic receptors was measured at 2.2 ± 0.7 nM. [less ▲]

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See detailBuilding an Effective Strategy To Supply High Frequency Stores in Emerging Cities
Sabiri, Asmaa; Riane, Fouad; Limbourg, Sabine ULg

in Cools, Mario; Limbourg, Sabine (Eds.) Proceedings of the BIVEC-GIBET Transport Research Days 2017: Towards an Autonomous and Interconnected Transport Future (2017)

Traditional distribution channels that cover nanostores still abound in merging megacities. These traditional retail formats need to be visited and supplied very frequently (High Frequency Stores) which ... [more ▼]

Traditional distribution channels that cover nanostores still abound in merging megacities. These traditional retail formats need to be visited and supplied very frequently (High Frequency Stores) which turns to be costly and time consuming if not well optimized. Supplying High Frequency Stores in emerging countries involves different constraints that make the associated optimization problems complex. In this paper, we deal with a real-life application problem where a company try to build an effective strategy to cover and to supply nanostores. We present an altered approach to a multi-depot multiple traveling salesman problem. The problem of concern is modeled as an assignment problem, combined with side constraints regarding disruption of pre-assignments and profit potential balance. The results show that the multi-objective function produces better profit and workload balance than setting a simple objective function and a constraint, one for the profit balance and the other for workload balance and vice-versa. [less ▲]

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See detailThe Fleet Size and Mix Vehicle Routing Problem with sequence-based pallet loading and axle weight constraints
Pollaris, Hanne; Braekers, Kris; caris, An et al

in Cools, Mario; Limbourg, Sabine (Eds.) Proceedings of the BIVEC-GIBET Transport Research Days 2017: Towards an Autonomous and Interconnected Transport Future (2017)

In this paper, the Fleet Size and Mix Vehicle Routing Problem (FSM) with sequence-based pallet loading and axle weight constraints is introduced. The effect of the integration of axle weight constraints ... [more ▼]

In this paper, the Fleet Size and Mix Vehicle Routing Problem (FSM) with sequence-based pallet loading and axle weight constraints is introduced. The effect of the integration of axle weight constraints in a FSM is analyzed by comparing the FSM with and without axle weight constraints. A vehicle fleet of 30-foot and 45-foot trucks, consisting of a tractor and a semi-trailer, is considered. Furthermore, two scenarios are analyzed for which the objective function differs: in the first scenario the objective is to minimize total distance while in the second scenario the objective is the minimization of total transport costs. An Iterated Local Search metaheuristic algorithm is used to tackle the problem. The results indicate that the vehicle fleet strongly influences the impact of the integration of axle weight constraints on solution cost. Furthermore, the analysis points out that fleet size and mix decisions may be influenced by the integration of axle weight constraints. [less ▲]

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See detailDes friches minières comme capital rural ?
Schmitz, Serge ULg

Conference (2017, May)

La communication a pour but de proposer un typologie théorique des friches minières et d'interroger leur potentiel de développement.

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See detailEvaluation of the implementation of integrated care for people with chronic conditions
Van Durme, Thérèse; Anthierens, Sibyl; Van Hecke, Ann et al

Conference (2017, May)

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See detailDiversity in sequences, post-translational modifications and expected pharmacological activities of toxins from four Conus species revealed by the combination of cutting-edge proteomics, transcriptomics and bioinformatics
Degueldre, Michel; Verdenaud, Marion; Garikoitz, Legarda et al

in Toxicon (2017), 130

Venomous animals have developed a huge arsenal of reticulated peptides for defense and predation. Based on various scaffolds, they represent a colossal pharmacological diversity, making them top ... [more ▼]

Venomous animals have developed a huge arsenal of reticulated peptides for defense and predation. Based on various scaffolds, they represent a colossal pharmacological diversity, making them top candidates for the development of innovative drugs. Instead of relying on the classical, low-throughput bioassay-guided approach to identify innovative bioactive peptides, this work exploits a recent paradigm to access to venom diversity. This strategy bypasses the classical approach by combining high-throughput transcriptomics, proteomics and bioinformatics cutting-edge technologies to generate reliable peptide sequences. The strategy employed to generate hundreds of reliable sequences from Conus venoms is deeply described. The study led to the discovery of (i) conotoxins that belong to known pharmacological families targeting various GPCRs or ion-gated channels, and (ii) new families of conotoxins, never described to date. It also focusses on the diversity of genes, sequences, folds, and PTM's provided by such species. [less ▲]

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See detailLes pouvoirs publics et les édifices cultuels en Belgique
Husson, Jean-François ULg

in Revue du Droit des Religions (2017), (3), 61-78

The Belgian regime of relations between the State and religious or philosophical communities results notably in financial supports for buildings used for worship and moral counselling. These interventions ... [more ▼]

The Belgian regime of relations between the State and religious or philosophical communities results notably in financial supports for buildings used for worship and moral counselling. These interventions are essentially a legacy of the French Concordat, largely unchallenged by the regionalization process. Today, it has to respond to contrasting situations between religions recognized in the 19th century – generally declining – and more recently recognized ones – generally expanding –. An additional complication originates in differences in ownership of the buildings or their classification as listed buildings. After presenting the situation by religious and philosophical community and level of power, this paper questions the equity of the system and addresses the possible developments. [less ▲]

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See detailL'intrapreneuriat, vecteur de nouvelles formes de collaborations ?
Lisein, Olivier ULg

Conference (2017, May)

Intervention sur les enjeux, les challenges et les bénéfices potentiels de l'intrapreneuriat comme vecteur d'innovations et de collaborations pour et au sein des entreprises. Intervention réalisée lors de ... [more ▼]

Intervention sur les enjeux, les challenges et les bénéfices potentiels de l'intrapreneuriat comme vecteur d'innovations et de collaborations pour et au sein des entreprises. Intervention réalisée lors de la table ronde "Interview d’experts: Les nouvelles pratiques de management dans l’entreprise collaborative" dont le fil conducteur est résumé comme suit : "Face à un marché en pleine mutation, nous verrons lors de cette table ronde quelles actions de management les entreprises mettent en place pour valoriser les individus et développer leurs compétences, mais aussi pérenniser une relation empreinte d’autonomie". [less ▲]

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See detailIsomeric species detection of base oil hydrocarbons by photo ionization GC×GC-HRTOF-MS
Giri, Anupam ULg; Courtiade, M; Racaud, A et al

Conference (2017, May)

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See detailThe internet as a source of information used by women after childbirth to meet their need for information: A web-based survey.
Slomian, Justine ULg; Bruyère, Olivier ULg; Reginster, Jean-Yves ULg et al

in Midwifery (2017), 48

OBJECTIVE: the aims of this survey were: (a) to evaluate the need of information after childbirth and what questions do 'new' mothers ask themselves; (b) to assess why and how women use the Internet to ... [more ▼]

OBJECTIVE: the aims of this survey were: (a) to evaluate the need of information after childbirth and what questions do 'new' mothers ask themselves; (b) to assess why and how women use the Internet to meet their need of information; (c) to describe how the respondents evaluate the reliability of the information found; (d) to understand how the information found on the Internet affects women's decision-making; and (e) to appreciate how health professionals react to the information found by the women. DESIGN: this study used a large web-based survey that was widely broadcasted on various websites and social networks. SETTING AND PARTICIPANTS: belgian women who had a child under 2 years old and who agreed to participate were included in the study. FINDINGS: 349 questionnaires were valid for analyses. After childbirth, 90.5% of women admitted to using the Internet to seek information about themselves or about their baby, regardless of socioeconomic status or age. There were various reasons for seeking information on the Internet, but the most frequent reason the women expressed was to find information 'on their own' (88.1%). The most searched for topic was breastfeeding. The women believed that the information was quite useful (82.7%) but they assigned an average score of 5.3 out of 10 for the quality of the information they found on the Internet. Approximately 80% of the women felt that the Internet helped them control a decision that they made 'a little', 'often' or 'very often'. Professionals are not always willing to talk about information found on the Internet with mothers. Therefore, many women believed that health professionals should suggest reliable Internet websites for new mothers. CONCLUSIONS: the integration of the Internet and new technologies could be a useful tool during postpartum management. [less ▲]

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See detailEffect of Multicolumn Lead Spinal Cord Stimulation on Low Back Pain in Failed Back Surgery Patients: A Three-Year Follow-Up
Remacle, Thibault ULg; BONHOMME, Vincent ULg; Renwart, Henri-Jean et al

in Neuromodulation (2017)

OBJECTIVES:Spinal cord stimulation (SCS) remains poorly efficient at reducing back pain in failed back surgery syndrome (FBSS) patients. We aimed at determining whether a new multicolumn lead SCS ... [more ▼]

OBJECTIVES:Spinal cord stimulation (SCS) remains poorly efficient at reducing back pain in failed back surgery syndrome (FBSS) patients. We aimed at determining whether a new multicolumn lead SCS technique was efficient at durably reducing their leg (LP) and back (BP) pain. MATERIALS AND METHODS:Sixty-two consecutive refractory FBSS patients received multicolumn SCS. Visual analogue scale (VAS) self-evaluation of BP, LP, and limitation of daily activity (LAD) were recorded preoperatively and at 2, 6, 12, 24, and 36 months after surgery. Quality of sleep and use of concomitant medications were also recorded. RESULTS:Complete datasets were obtained in 29 patients. BP (median VAS [25-75 centiles]) significantly decreased from 9 (8.5-10) preoperatively, to 3 (3-4) at short-term follow-up (2 months), and rose up to 5 (4-5) at 36 months. LP evolved from 7 (6-8) preoperatively, to 2.5 (2-3) at short-term follow-up, and 3 (2-3) at 36 months. Correspondingly, LAD VAS was 8 (8-9) preoperatively, and decreased to stable values of 3 (3-4) during the follow-up period. Quality of sleep also improved, with 72% of patients reporting poor sleep preoperatively to 0-7% in the follow-up period until 36 months. The percentages of patients regularly taking analgesic and/or co-analgesic medications decreased from 100% preoperatively to 8, 4, 12, 19, and 19%. CONCLUSION:Multicolumn lead SCS in FBSS patients significantly improve BP, LP, quality of life, and medication consumption for at least 36 months. A classical placebo effect cannot account for long-term improvements of such magnitude. [less ▲]

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See detailIsotopic half-life and enrichment factor in two species of European freshwater fish larvae: an experimental approach
Latli, Adrien; Sturaro, Nicolas ULg; Dujardin, Nelson et al

in Rapid Communications in Mass Spectrometry (2017), 31(8), 685-692

RATIONALE: Stable isotope ratios of carbon and nitrogen are valuable tools for field ecologists to use to analyse animal diets. However, the application of these tools requires knowledge of the tissue ... [more ▼]

RATIONALE: Stable isotope ratios of carbon and nitrogen are valuable tools for field ecologists to use to analyse animal diets. However, the application of these tools requires knowledge of the tissue enrichment factor (TEF) and half-life (HL). We experimentally compared TEF and HL in two freshwater fish larvae. We hypothesised that chub had a better growth/tissue replacement ratio than roach, due to the use of a food closer to their natural diet. METHODS: We determined the isotopic HL, the TEF and the contribution of growth or metabolic tissue replacement to dynamic isotopic incorporation. After yolk sac resorption, larvae were fed for 5 weeks with prey similar to their natural diet (Artemia nauplii) up to the isotopic equilibrium followed by Chironomid larvae. Stable isotope measurements were carried out using a continuous flow isotope ratio mass spectrometer coupled to an elemental analyser. RESULTS: Changes in isotopic composition strongly followed the predictions of exponential growth and time-dependent models. The isotopic HL varied between 8.2 and 12.6 days and the TEF of nitrogen and carbon ranged from 1.7 to 2.1‰ and from –0.9 to 1.2 ‰, respectively. The incorporation of dietary 13C was due more to the production of new tissue (between 56 and 79%) than to the metabolic process. Chub allocated more energy to growth than roach and the Chironomidae diet contributed more to the consumers’ growth than the Artemia diet. CONCLUSIONS: Metabolic rates seemed lower for chub than for roach, especially when they were fed with Chironomidae. A Chironomidae-based diet would be more profitable to chub, and the high associated growth rate could increase the development of the fish larvae. The HL and TEF were in the range of those reported in the literature. These results will be helpful for field-based studies, because they can help to increase the accuracy of models. [less ▲]

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See detailL'enseignement-apprentissage du français dans le qualifiant : regards croisés
Scheepers, Caroline ULg; Outers, Pierre ULg; Werner, Anne-Catherine ULg

Poster (2017, April 29)

La présentation donne à voir les résultats partiels d'une recherche exploratoire qui a consisté à questionner les élèves et les professeurs de l'enseignement secondaire de transition et du qualifiant à ... [more ▼]

La présentation donne à voir les résultats partiels d'une recherche exploratoire qui a consisté à questionner les élèves et les professeurs de l'enseignement secondaire de transition et du qualifiant à propos de leurs représentations relatives à l'enseignement-apprentissage du français dans le qualifiant. Pour l'heure, nous disposons des réponses apportées par 30 enseignants et par 450 élèves. A terme, cinquante enseignants et 1000 élèves sont concernés. [less ▲]

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See detailEffect of growth rate on the physical and mechanical properties of Douglas-fir in Western Europe
Polet, Caroline; Henin, Jean-Marc; Hebert, Jacques ULg et al

in Canadian Journal of Forest Research = Journal Canadien de la Recherche Forestière (2017), 47

To quantify the impact of forest management and tree growth rate on the potential uses of Douglas-fir wood, nine physico-mechanical properties were studied on more than 1250 standardized clear specimens ... [more ▼]

To quantify the impact of forest management and tree growth rate on the potential uses of Douglas-fir wood, nine physico-mechanical properties were studied on more than 1250 standardized clear specimens. These were collected from trees cut in 11 even-aged stands (6 trees/stand) located in Wallonia (Southern Belgium). Stands were 40 to 69 years old and the mean tree girth was ca. 150 cm. The mean ring width of the 66 trees ranged from 3 to more than 7 mm. Statistical analysis evidenced significant but weak effects of ring width. So, mean ring width and cambial age of the specimens considered jointly only explain 28 to 40% of the variability of the properties studied. From a purely technological standpoint, maintaining mean ring width under 4 mm/year in juvenile wood and 6 mm/year in mature wood should allow all potential uses of Douglas-fir wood. Our results and the literature demonstrate, however, the importance of genetic selection as a complement of silvicultural measures to improve or guarantee the technological properties of Douglas-fir wood. [less ▲]

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See detailDes preuves : où, quand, comment ?
Rigo, Michel ULg

Speech/Talk (2017)

Expliquer une démonstration, prouver un résultat, énoncer une conjecture sont des activités qui font partie intégrante du quotidien du mathématicien. Dans ce court exposé, je passerai en revue quelques ... [more ▼]

Expliquer une démonstration, prouver un résultat, énoncer une conjecture sont des activités qui font partie intégrante du quotidien du mathématicien. Dans ce court exposé, je passerai en revue quelques exemples de preuves. Certaines sont classiques ou historiques, d'autres sont peut-être moins connues : des preuves à la Ramanujan, des preuves sans mots, le théorème des quatre couleurs, la conjecture de Klepler sur l'empilement de sphères,... Le but est de partager mes réflexions comme chercheur « professionnel » et enseignant. [less ▲]

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See detailAmbulatory System for Gait Analysis
Boutaayamou, Mohamed ULg; Bruls, Olivier ULg; Croisier, Jean-Louis ULg et al

Conference (2017, April 29)

We describe the principle and use of a wireless, 3-axis accelerometer-based ambulatory system that records acceleration signals and automatically analyses them to characterize normal and pathological gait ... [more ▼]

We describe the principle and use of a wireless, 3-axis accelerometer-based ambulatory system that records acceleration signals and automatically analyses them to characterize normal and pathological gait. The associated algorithm is versatile enough to detect, on a stride-by-stride basis, refined gait parameters that quantify subtle gait disturbances in, e.g., in Parkinson’s disease in a rater-independent way. The experimental results show the potential of the developed accelerometer-based technique to be used in neurology (e.g., characterization of Parkinsonian gait: slowness, shuffling, short steps, freezing of gait, asymmetries in gait), rehabilitation, geriatrics (ex. monitoring activity parameters in the elderly), orthopedics and sport. [less ▲]

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