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See detailDynamique et composition des atmosphères supérieures de Mars et Vénus observées par les spectrographes ultraviolets à bord de Mars Express et Vénus Express
Stiepen, Arnaud ULg

Doctoral thesis (2014)

Venus has a dense CO2-dominated atmosphere. Above 50 km, the atmospheric circulation is composed of two dominants patterns: the Retrograde Superrotating Zonal (RSZ) circulation up to 65 km and the ... [more ▼]

Venus has a dense CO2-dominated atmosphere. Above 50 km, the atmospheric circulation is composed of two dominants patterns: the Retrograde Superrotating Zonal (RSZ) circulation up to 65 km and the subsolar to antisolar (SSAS) circulation beyond 120 km. The SSAS is caused by the temperature gradient between the day and the night hemispheres and it generates a global flow from the dayside to the nightside. Between 65 and 120 km, the circulation is complex as it is the superposition of the two main components. I have used the nightglow emitted by the NO* molecule (180-300 nm) produced by recombination of N(4S) and O(3P) atoms. These atoms are created by photodissociation of N2 and CO2 molecules on the dayside and carried to the nightside by the SSAS circulation. I analyze the full set of measurements of the NO δ and γ bands measured by the SPICAV instrument on board the ESA Venus Express spacecraft. I discuss the altitude and brightness of the emission peak (60 kR at 115 km) and show that the emission profile exhibits a secondary peak in ~10% of the cases. This additional peak is probably caused by upward-moving gravity waves propagating from below. I have developed an inversion method of the observed limb profiles to compare the results with a chemical-diffusive model of the atmosphere of Venus and constrain the vertical fluxes of N and O. On the basis of an extended statistical analysis, I characterize the presence of a bright spot of the nightglow of NO shifted from the antisolar point and located around 0230LT, 10°S. I use the vast SPICAV airglow database to statistically demonstrate that the NO nightglow is highly variable, both temporally and spatially. Mars experiences seasons as its rotation axis is inclined from its spin axis. Its upper atmospheric dynamics is dominated by summer-to-winter global transport. I study the upper atmosphere of Mars using nightglow observations performed by the SPICAM instrument on board ESA’s Mars Express spacecraft. I analyze the δ and γ bands of molecule NO in the nightside mesosphere to confirm, on statistical basis, the peak brightness and altitude of the NO UV emission (5 kR at 72 km). I show that the NO nightglow is located following the relation Latitude = -80xsin(Solar Longitude), in agreement with results based on stellar occultations and from the LMD model, which simulates the photochemistry and dynamics of the Mars atmosphere. I have performed a detailed study of the CO Cameron band (170 to 270 nm) and CO2+ doublet (290 nm) dayglow. I have developed a method to deduce the temperature profile around 150 km, an important study as the distribution of the temperature in the Mars atmosphere is poorly known. I showed that the temperature at high altitude is only weakly correlated with the solar EUV flux. This result suggests that the variability of the upper atmosphere may include internal processes. Comparisons with the Mars Global Ionosphere Thermosphere Model show that the model can reproduce the observation for high solar conditions but predicts lower temperature for low to moderate solar conditions. Further study will therefore be necessary to identify and understand the mechanisms that govern the temperature variability. [less ▲]

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See detailLa collecte des déchets en Wallonie : organisation et performances
Gautier, Axel ULg

Scientific conference (2014, April 03)

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See detailEU and Brazil : partners or competitors ?
Santander, Sébastian ULg

Scientific conference (2014, April 03)

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See detailRates of CTL Killing in Persistent Viral Infection In Vivo
Elemans, Marjet; Florins, Arnaud; Willems, Luc ULg et al

in PLoS Computational Biology (2014), 4

The CD8+ cytotoxic T lymphocyte (CTL) response is an important defence against viral invasion. Although CTL-mediated cytotoxicity has been widely studied for many years, the rate at which virus-infected ... [more ▼]

The CD8+ cytotoxic T lymphocyte (CTL) response is an important defence against viral invasion. Although CTL-mediated cytotoxicity has been widely studied for many years, the rate at which virus-infected cells are killed in vivo by the CTL response is poorly understood. To date the rate of CTL killing in vivo has been estimated for three virus infections but the estimates differ considerably, and killing of HIV-1-infected cells was unexpectedly low. This raises questions about the typical anti-viral capability of CTL and whether CTL killing is abnormally low in HIV-1. We estimated the rate of killing of infected cells by CD8+ T cells in two distinct persistent virus infections: sheep infected with Bovine Leukemia Virus (BLV) and humans infected with Human T Lymphotropic Virus type 1 (HTLV-1) which together with existing data allows us to study a total of five viruses in parallel. Although both BLV and HTLV-1 infection are characterised by large expansions of chronically activated CTL with immediate effector function ex vivo and no evidence of overt immune suppression, our estimates are at the lower end of the reported range. This enables us to put current estimates into perspective and shows that CTL killing of HIV-infected cells may not be atypically low. The estimates at the higher end of the range are obtained in more manipulated systems and may thus represent the potential rather than the realised CTL efficiency. [less ▲]

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See detailUse of on-line flow cytometry for the characterization of microbial stress dynamics during the bioprocess
Brognaux, Alison ULg; Han, Shanshan; Sorensen, Soren et al

Conference (2014, April 03)

Microbial cell population heterogeneity is now recognized as a major source of issues for the development and optimization of bioprocesses. Flow cytometry is a very powerful tool for the follow up of ... [more ▼]

Microbial cell population heterogeneity is now recognized as a major source of issues for the development and optimization of bioprocesses. Flow cytometry is a very powerful tool for the follow up of physiological properties of microbial cells in process-related conditions at the single cell level, and can be used to study the dynamics of segregation directly in bioreactors. In this context, specific interfaces have been developed in order to connect flow cytometer (FC) directly on bioreactor for automated analyses. In this work, we propose a simplified version of such interface and demonstrated its usefulness for multiplexed experiments. This automated FC system has been tested for the follow up of the dynamics of an E. coli pfis::gfpAAV fluorescent bio-reporter and its PI uptake, correlated with membrane permeability. This bioreporter is composed of a fis promoter, a growth dependent promoter-indicator of the nutrient status of cells, fused to a gene expressing an unstable variant of GFP. The results obtained showed that the dynamics of the GFP synthesis is complex and can be attributed to a complex set of biological parameters. Segregation in the membrane permeability has been noticed. This work demonstrates that a simplified version of on-line FC can be used at the process level for the investigation of the dynamics of complex physiological mechanisms. [less ▲]

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See detailNo simian Plasmodium detected in populations living in the equatorial rainy forest of the Democratic Republic of Congo
Mvumbi makaba, Dieudonné; Bobanga Lengu, Thierry; Kayembe Ntumba, Jean-Marie et al

Poster (2014, April 03)

Background Malaria remains the most deadly parasitic disease to date, especially in sub-Saharan Africa, which comprises the majority of cases collected per year. It has long been accepted that four ... [more ▼]

Background Malaria remains the most deadly parasitic disease to date, especially in sub-Saharan Africa, which comprises the majority of cases collected per year. It has long been accepted that four species of Plasmodium (P. falciparum, P. vivax, P. malariae and P. ovale) were responsible for the disease in humans. But quite recently, a fifth species, Plasmodium Knowlesi, has been identified as naturally infecting humans. Indeed, known for decades as naturally parasitizing the monkey Macaca fascicularis, P. knowlesi has long been confused, in terms of its evolutionary stage, with P. malariae or P. falciparum, which it resembles morphologically and it was not possible to properly differentiate them until the advent of molecular biology. To date, P. Knowlesi has only been identified in Southeast Asia and a similar phenomenon of natural transmission of simian plasmodium to humans has not been reported elsewhere. We therefore conducted this study to investigate the possible transmission of simian plasmodium to humans in populations living near the rainforest of the Democratic Republic of Congo (DRC) where several species of primates lives. Methods & Materials Three villages (Wenji-Secli, Bongonde, and Bolenge) in the Province of Ecuador (North-eastern DRC) were selected because of their geographical location. Blood samples spotted on filter paper were collected from 100 people randomly taken in each village. Two successive RT- PCR were performed. A first one using a single probe able to diagnose all plasmodium spp. and a second using four species-specific probes for the diagnosis of the four conventional human plasmodium species. Positivity in the first RT- PCR with negativity in the second RT- PCR would suggest the presence of plasmodium species other than the four conventional. Results P. falciparum was correctly identified in 44.6 % of samples. No other species of human plasmodium or not has been identified. Conclusion This preliminary study did not detect the presence of simian plasmodium in human populations living in the rainforest of the DRC. Studies with larger samples and with more advanced techniques should still be conducted. Keywords: Malaria, simian plasmodium, DR Congo [less ▲]

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See detailLa confiance comme processus relationnel. Une tentative de modélisation
Dethier, Baptiste ULg

Scientific conference (2014, April 03)

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See detailCaractérisation minéralogique, géochimique et géotechnique des matières premières utilisées en poterie dans le site d’Agafay, Marrakech, Maroc.
El Boudour El Idrissi, Hicham; Daoudi, Lahcen; El Ouahabi, Meriam ULg et al

Conference (2014, April 03)

Le site potier d’Agafay est localisé à 20 km au S-W de la ville de Marrakech, à l’ouest de l’oued N’Fis. L’activité potière y a débuté par transfert du savoir-faire à partir du village de Tamsloht vers ... [more ▼]

Le site potier d’Agafay est localisé à 20 km au S-W de la ville de Marrakech, à l’ouest de l’oued N’Fis. L’activité potière y a débuté par transfert du savoir-faire à partir du village de Tamsloht vers 1940 apr. J.-C. Le site comporte environ 90 ateliers où sont actifs 240 artisans. Les principales pièces produites sont les gasriyas (i.e., plats traditionnels de couscous et de modelage de pâtes de pains) et les briques des grandes murailles traditionnelles. Les matières premières utilisées, brutes ou en mélange, sont issues des terres agricoles locales (Ag1 et Ag5) pour les gasriyas et des dépôts actuels du oued N’Fis (Ag7) pour les briques. Les problématiques rencontrées dans ce site concernent les deux types de produits, gasriyas et briques. Dans le premier cas, des grains de la chaux gonflent dans les pièces après le contact prolongé des pièces produites avec l’air. Ce gonflement engendre des décollements dans les tessons et nuit à la qualité du produit final. Dans le deuxième cas, la distribution non homogène de la chaleur à l’intérieur des fours traditionnels utilisés engendre une très grande variation de couleur dans les briques, elles sont généralement tordues et certaines fondent totalement. Notre projet vise à une caractérisation scientifique des différentes phases de fabrication afin de remédier à ces problèmes. Les résultats montrent que la composition chimique est surtout silico-alumineuse (55 à 61 % de SiO2 ; 16 à 20% d’Al2O3), la teneur en Fe2O3 se situe entre 6 et 8 %, celle de CaO ne dépasse pas 6% pour les pâtes des gasriyas et atteint 10 % pour les pâtes des briques. La teneur en carbonates mesurée par le calcimètre de Bernard varie entre 5 et 14 %. La teneur en matière organique varie entre 5 et 7,5 %. L’indice de plasticité oscille entre 16 et 17,5. Pour des teneurs en eau proches de la limite de plasticité le degré de retrait au séchage des gasriyas est de l’ordre de 5 %, il est plus élevé pour les briques (8%) ce qui nécessite une plus grande maitrise du processus de séchage pour éviter que les pièces ne se tordent pas. La granulométrie des pâtes utilisées pour les gasriyas a montré une teneur moyenne de 50 % pour les argiles, 32 % pour les limons et 18 % pour les sables. Les briques comportent une proportion égale des trois classes granulométriques. La minéralogie de l’ensemble des pâtes est formée de quartz (< 27%), plagioclase (13%), calcite (7 à 9%), dolomite (5%) et argile (50%, 34% et 34% pour Ag1, Ag5 et Ag7, respectivement). Le feldspath potassique est présent uniquement dans l’échantillon Ag7. Concernant la fraction argileuse, elle est surtout constituée d’illite pour l’ensemble des pâtes, associée à des interstratifiés irréguliers 14c-14s (13 %) pour la pâte des briques, et une teneur de 11 % de smectite pour la pâte des gasriyas (Ag5). La mesure de la vitesse sonique a montré que les pièces formées par pression aboutissent à une porosité moindre que celle des pièces produites par boudinage. La densité des pièces produites reste semblable pour les cuissons à 950°C et 1050°C. Le plus bas degré de porosité ainsi que les plus grandes valeurs de résistance mécanique à la compression ont été enregistrés sur les pièces cuites à 1050 °C. Les contraintes maximales atteintes se situent entre 51 et 59 MPa pour les gasriyas et 24,5 MPa pour les briques. Quant à la résistance mécanique à la flexion, elle varie entre 17,4 et 20,3 N/mm² pour les gasriyas et 9,4 N/mm² pour les briques. L’analyse DRX des pièces cuites à 1050 °C a montré la disparition totale des carbonates et des argiles à cette température, accompagnée de l’apparition de la géhelénite, cristobalite, anorthoclase, diopside, spinelle et hématite. A l’issue de cette étude, nous recommandons un tamisage humide à 1 mm pour réduire l’effet des gros grains de la chaux, une cuisson par palier jusqu’à 1050°C est également nécessaire pour comprendre les transformations des phases minéralogiques et vitreuses. Finalement, l’utilisation des fours équipés d’une sonde pour garantir une meilleure distribution de la chaleur. [less ▲]

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See detailNest grouping patterns of bonobos (Pan paniscus) in relation to fruit availability in a forest-savannah mosaic
Serckx, Adeline ULg; Huynen, Marie-Claude ULg; Bastin, Jean-François ULg et al

in PLoS ONE (2014)

A topic of major interest in socio-ecology is the comparison of chimpanzees and bonobos’ grouping patterns. Numerous studies have highlighted the impact of social and environmental factors on the ... [more ▼]

A topic of major interest in socio-ecology is the comparison of chimpanzees and bonobos’ grouping patterns. Numerous studies have highlighted the impact of social and environmental factors on the different evolution in group cohesion seen in these sister species. We are still lacking, however, key information about bonobo social traits across their habitat range, in order to make accurate inter-species comparisons. In this study we investigated bonobo social cohesiveness at nesting sites depending on fruit availability in the forest-savannah mosaic of western Democratic Republic of Congo (DRC), a bonobo habitat which has received little attention from researchers and is characterized by high food resource variation within years. We collected data on two bonobo communities. Nest counts at nesting sites were used as a proxy for night grouping patterns and were analysed with regard to fruit availability. We also modelled bonobo population density at the site in order to investigate yearly variation. We found that one community density varied across the three years of surveys, suggesting that this bonobo community has significant variability in use of its home range. This finding highlights the importance of forest connectivity, a likely prerequisite for the ability of bonobos to adapt their ranging patterns to fruit availability changes. We found no influence of overall fruit availability on bonobo cohesiveness. Only fruit availability at the nesting sites showed a positive influence, indicating that bonobos favour food ‘hot spots’ as sleeping sites. Our findings have confirmed the results obtained from previous studies carried out in the dense tropical forests of DRC. Nevertheless, in order to clarify the impact of environmental variability on bonobo social cohesiveness, we will need to make direct observations of the apes in the forest-savannah mosaic as well as make comparisons across the entirety of the bonobos’ range using systematic methodology. [less ▲]

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See detailBayesian inference of a dynamic vegetation model for grassland
Minet, Julien ULg; Laloy, Eric; Tychon, Bernard ULg et al

Conference (2014, April 02)

As a part of the MACSUR task L2.4, we probabilistically calibrated the CARAIB dynamic vegetation model by Markov chain Monte Carlo (MCMC) simulation with the DREAMZS sampler.. CARAIB is a mechanistic ... [more ▼]

As a part of the MACSUR task L2.4, we probabilistically calibrated the CARAIB dynamic vegetation model by Markov chain Monte Carlo (MCMC) simulation with the DREAMZS sampler.. CARAIB is a mechanistic model that calculates the carbon assimilation of the vegetation as a function of the soil and climatic conditions, and can thus be used for simulating grassland production under cutting or grazing management. Bayesian model inversion was performed at 4 grassland sites across Europe: Oensingen, CH; Grillenburg, DE; Laqueuille, FR and Monte-Bodone, IT. Four daily measured variables from these sites: the Gross Primary Productivity (GPP), Net Ecosystem Exchange (NEE), Evapotranspiration (ET) and Soil Water Content (SWC) were used to sample 10 parameters related to rooting depth, stomatal conductance, specific leaf area, carbon-nitrogen ratio and water stresses. The maximized likelihood function therefore involved four objectives, whereas the applied Bayesian framework allowed for assessing the so called parameter posterior probability density function (pdf), which quantifies model parameter uncertainty caused by measurement and model errors. Sampling trials were performed using merged data from all sites (all-sites-sampling) and for each site (site-specific sampling) separately. The derived posterior parameter pdfs from the all-sites sampling and site-specific sampling runs showed differences in relation with the specificities of each site. Analysis of these distributions also revealed model sensitivity to parameters conditioned on the measured data, as well as parameter correlations. [less ▲]

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See detailWill top-down interactions be affacted by climate change?
Boullis, Antoine ULg; Verheggen, François ULg; Francis, Frédéric ULg

Poster (2014, April 02)

Greenhouse gas emissions are rising since the beginning of the industrial era: atmospheric carbon dioxide now exceeds 400 ppm (30% increase since the mid-1800s) and tropospheric ozone has reached 40 ppb ... [more ▼]

Greenhouse gas emissions are rising since the beginning of the industrial era: atmospheric carbon dioxide now exceeds 400 ppm (30% increase since the mid-1800s) and tropospheric ozone has reached 40 ppb in industrialized areas. According to forecasting models, these concentrations are expected to double by the end of the 21st century. To date, impacts of elevated greenhouse gases have been extensively studied in plant-insect interactions, whereas interactions between herbivores and their natural enemies have been neglected. Because most interactions between herbivorous insects and their natural enemies are orchestrated by natural molecules produced as semiochemicals, we hypothesize that modification in atmospheric gas composition will alter chemical communication in insects, and affect the performance of natural enemies to locate their prey. In this project, we shall attempt to analyze the effect of increase in greenhouse gas concentrations on insect communication, particularly in top-down interactions. The study model will consist in the interactions between the pea aphids (Acyrthosiphon pisum (Harris)) and their natural enemies Harmonia axyridis (Pallas) and Episyrphus balteatus (DeGeer). Under elevated CO2 and/or O3 concentrations, we will quantify the production of several aphid semiochemicals that are currently used as kairomones by natural enemies, including the aphid alarm pheromone E-β-farnesene and honeydew volatile compounds. Olfactometry assays will then be set up to examine the ability of natural enemies to locate their prey in the predicted atmospheric conditions of the end of the 21st century. Finally, using an Ecotron structure to be built in Gembloux Agro-Bio Tech, we will simulate a future ecosystem with the possibility to control additional biotic and abiotic parameters. [less ▲]

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See detailBiological control of aphids in wheat and vegetable crops : a multi-approach case study in Shandong province (China)
Chevalier Mendes Lopes, Thomas ULg; Bosquée, Emilie ULg; Chen, Julian et al

Poster (2014, April 02)

This multi-approach study was conducted in the Shandong province (China) to assess the effectiveness of different biological control methods against aphids in wheat and vegetable crops. Three approaches ... [more ▼]

This multi-approach study was conducted in the Shandong province (China) to assess the effectiveness of different biological control methods against aphids in wheat and vegetable crops. Three approaches were tested: (1) wheat/oilseed rape and wheat/pea associations, (2) potatoes/peas association, and (3) E-β-farnesene (aphid alarm pheromone) releasers in squashes under plastic tunnels. Aphids and aphidophagous beneficials were monitored by observations on plants. Wheat associations and E-β-farnesene reduced aphid infestations compared with control treatments, while no effect was observed in the potatoes/peas association. Moreover, aphidophagous beneficials were significantly more abundant in the three kinds of associations compared with control treatments. Few individuals were observed on squashes. Additionally, adult aphidophagous beneficials were monitored using yellow pan traps. The parasitoid species Aphidius gifuensis (Ashmead) was prevalent, while Propylea japonica (Thunberg) and Harmonia axyridis (Pallas) were the most abundant species among predators. Globally, wheat associations were more efficient to reduce aphid infestations and attract aphidophagous beneficials to promote biological control. The trapping of natural enemies allowed to assess the diversity and abundance of aphidophagous species in this region. This study shows a beneficial effect of crop associations and E-β-farnesene on the control of aphid populations and contributes to assess the potentialities of these alternative methods to the use of insecticides. [less ▲]

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See detailConservatoire entomologique de Gembloux Agro-Bio Tech: les coccinelles de Wallonie et de Bruxelles de 2001 à 2009
Vandereycken, Axel ULg; Fassotte, Bérénice ULg; Durieux, Delphine ULg et al

Poster (2014, April 02)

Un inventaire des Coccinellidae présents au sein de la collection de l’unité d’Entomologie fonctionnelle et évolutive (ULg - Gembloux Agro-Bio Tech) a été réalisé sur la période s’étendant de 2001 à 2009 ... [more ▼]

Un inventaire des Coccinellidae présents au sein de la collection de l’unité d’Entomologie fonctionnelle et évolutive (ULg - Gembloux Agro-Bio Tech) a été réalisé sur la période s’étendant de 2001 à 2009. Cette collection est essentiellement constituée par les récoltes des étudiants de première Bachelier de Gembloux Agro-Bio Tech. A travers ce recensement, nous avons étudié l’évolution des espèces récoltées au sein de cette famille en termes d’effectif relatif et de richesse spécifique. Notre étude s’est focalisée sur les données issues de Wallonie et de la Région de Bruxelles-Capitale, trop peu de collectes ayant été réalisées en Flandre. Parmi les individus identifiés, 27 espèces ont été observées, dont 21 appartiennent à la sous-famille des Coccinellinae, 2 à celle des Epilachninae et 4 à celle des Chilocorinae. La plupart des espèces sont communes à la Belgique. Cependant, Harmonia axyridis (Pallas 1773), espèce originaire du sud-est de l’Asie, a été introduite sur le territoire belge en 1997. Cette coccinelle exotique fait son apparition dans les récoltes de 2002 et son effectif ne cesse d’augmenter au fil des années prospectées. En parallèle à cette augmentation, une diminution de la richesse spécifique, ainsi que de l’effectif relatif d’Adalia bipunctata (L. 1758), de Propylea quatuordecimpunctata (L. 1758) et de Psyllobora vigintiduopunctata (L. 1758), est observée. [less ▲]

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See detailImages fixes, cadre mobile. Fantômes de cinéma dans la bande dessinée expérimentale contemporaine
Belloi, Livio ULg

Conference (2014, April 02)

La présente communication porte sur la bande dessinée expérimentale contemporaine et se focalise plus particulièrement sur "Travelling Square District" (2010) du jeune dessinateur belge Greg Shaw. Objet ... [more ▼]

La présente communication porte sur la bande dessinée expérimentale contemporaine et se focalise plus particulièrement sur "Travelling Square District" (2010) du jeune dessinateur belge Greg Shaw. Objet très singulier, cet album se présente comme une expérimentation systématique autour du cadre mobile, dans un art fondé sur des successions d'images fixes. En cela, cette œuvre peut être considérée comme relevant d'une para-histoire du cinéma. [less ▲]

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See detailApport des examens biologiques en pratique allergologique
GADISSEUR, Romy ULg

Conference (2014, April 02)

Apport des examens biologiques en pratique allergologique

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