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See detailUne architecture de frontières : les monastères des annonciades célestes dans les Trois-Evêchés (17e-18e siècles)
Piront, Julie ULg

in Bourdieu, Catherine (Ed.) Vie religieuse féminine dans les Trois-Evêchés : vocations, missions et cadres d'existence (2017)

Les Trois-Evêchés ont été un terrain fertile pour les congrégations féminines aux 16e-18e siècles puisqu'ils ont vu la naissance de projets religieux conjuguée à une installation massive de « nouveaux ... [more ▼]

Les Trois-Evêchés ont été un terrain fertile pour les congrégations féminines aux 16e-18e siècles puisqu'ils ont vu la naissance de projets religieux conjuguée à une installation massive de « nouveaux ordres » nés ou réformés dans le sillage du concile de Trente. Fondées en Italie au début du 17e siècle, les annonciades célestes se sont implantées majoritairement sur la « dorsale catholique ». Au cœur de ce territoire de frontières, les Trois-Évêchés occupent une position géographique, politique et religieuse incontournable. Dans le cadre de cette journée d’études, il s’agira d’interroger les spécificités éventuelles des cinq monastères que les annonciades célestes firent édifier dans les Trois-Évêchés en les comparant à l’ensemble de la production architecturale de l’ordre en Europe aux 17e et 18e siècles. En allant plus loin, nous verrons comment les représentations mentales de ces édifices ont participé à la genèse de l’identité de l’ordre, notamment grâce aux sources écrites et iconographiques qui en témoignent. [less ▲]

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See detailMembrane Interactions of Natural Cyclic Lipodepsipeptides of the Viscosin Group
Geudens, Niels; Nasir, Mehmet Nail ULg; Crowet, Jean-Marc ULg et al

in Biochimica et Biophysica Acta - Biomembranes (2017), 1859(3), 331-339

Many Pseudomonas spp. produce cyclic lipodepsipeptides (CLPs), which, besides their role in biological functions such as motility, biofilm formation and interspecies interactions, are antimicrobial. It ... [more ▼]

Many Pseudomonas spp. produce cyclic lipodepsipeptides (CLPs), which, besides their role in biological functions such as motility, biofilm formation and interspecies interactions, are antimicrobial. It has been established that interaction with the cellular membrane is central to the mode of action of CLPs. In this work, we focus on the CLPs of the so-called viscosin group, aiming to assess the impact of the main structural variations observed within this group on both the antimicrobial activity and the interaction with model membranes. The antimicrobial activity of viscosin, viscosinamide A, WLIP and pseudodesmin A were all tested on a broad panel of mainly Gram-positive bacteria. Their capacity to permeabilize or fuse PG/PE/cardiolipin model membrane vesicles is assessed using fluorescent probes. We find that the Glu2/Gln2 structural variation within the viscosin group is the main factor that influences both the membrane permeabilization properties and the minimum inhibitory concentration of bacterial growth, while the configuration of the Leu5 residue has no apparent effect. The CLPmembrane interactions were further evaluated using CD and FT-IR spectroscopy on model membranes consisting of PG/PE/cardiolipin or POPC with or without cholesterol. In contrast to previous studies, we observe no conformational change upon membrane insertion. The CLPs interact both with the polar heads and aliphatic tails of model membrane systems, altering bilayer fluidity, while cholesterol reduces CLP insertion depth [less ▲]

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See detailTwo new species in the Micarea prasina group from Western Europe
van den Boom, Pieter P. G.; Brand, A. Maarten; Coppins, Brian J. et al

in Lichenologist (2017), 49(1), 13-25

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See detailShip collision analysis on Offshore Wind Turbine monopile foundations
Bela, Andreea ULg; Le Sourne, Hervé; Buldgen, Loïc ULg et al

in Marine Structures (2017), 51

An offshore wind farm covers a large maritime area and the site can be located close to traffic lanes of commercial and passenger ships, which may lead to possible collision events. The work presented in ... [more ▼]

An offshore wind farm covers a large maritime area and the site can be located close to traffic lanes of commercial and passenger ships, which may lead to possible collision events. The work presented in this paper aimed to understand both the crushing behavior and the nacelle dynamics of a predefined monopile offshore wind turbine when impacted by a ship. Another objective was to deeply investigate the influence of various parameters like ship impact velocity and location, wind direction, soil stiffness and deformability of the striking ship. First, nonlinear numerical simulations of ship - Offshore Wind Turbine (OWT) collisions have been carried out with a rigid striking ship for a better understanding of the OWT’s structural behaviour during collision. Different configurations for the wind turbine’s structure have been used in order to highlight the modifications in behaviour induced by changing the soil conditions or the loading scenario. The resulting resistant force and internal energies have been compared as well as the tower top (nacelle) displacements and accelerations. Then, another series of simulations have been performed with a deformable ship in order to investigate the influence of the deformability of the striking ship on the OWT’s behaviour. It is worth noting that all these numerical results will further serve to fix the hypotheses for the development of a simplified tool based on analytical formulations. [less ▲]

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See detailCXCL12 mediates glioblastoma resistance to radiotherapy in the subventricular zone.
Goffart, Nicolas ULg; Lombard, Arnaud; Lallemand, François ULg et al

in Neuro-Oncology (2017), 19(1), 66-77

BACKGROUND: Patients with glioblastoma (GBM) have an overall median survival of 15 months despite multimodal therapy. These catastrophic survival rates are to be correlated to systematic relapses that ... [more ▼]

BACKGROUND: Patients with glioblastoma (GBM) have an overall median survival of 15 months despite multimodal therapy. These catastrophic survival rates are to be correlated to systematic relapses that might arise from remaining glioblastoma stem cells (GSCs) left behind after surgery. In this line, it has recently been demonstrated that GSCs are able to escape the tumor mass and preferentially colonize the adult subventricular zone (SVZ). At a distance from the initial tumor site, these GSCs might therefore represent a high-quality model of clinical resilience to therapy and cancer relapses as they specifically retain tumor-initiating abilities. METHOD: While relying on recent findings that have validated the existence of GSCs in the human SVZ, we questioned the role of the SVZ niche as a potential GSC reservoir involved in therapeutic failure. RESULTS: Our results demonstrate that (i) GSCs located in the SVZ are specifically resistant to radiation in vivo, (ii) these cells display enhanced mesenchymal roots that are known to be associated with cancer radioresistance, (iii) these mesenchymal traits are specifically upregulated by CXCL12 (stromal cell-derived factor-1) both in vitro and in the SVZ environment, (iv) the amount of SVZ-released CXCL12 mediates GBM resistance to radiation in vitro, and (v) interferes with the CXCL12/CXCR4 signalling system, allowing weakening of the tumor mesenchymal roots and radiosensitizing SVZ-nested GBM cells. CONCLUSION: Together, these data provide evidence on how the adult SVZ environment, through the release of CXCL12, supports GBM therapeutic failure and potential tumor relapse. [less ▲]

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See detailThe somatotrope Growth Hormone-Releasing Hormone/Growth Hormone/Insulin-like Growth Factor 1 axis in immunoregulation and immunosenescence
Bodart, Gwennaëlle ULg; Farhat, Khalil; RENARD, Jeanne de Chantal ULg et al

in Savino, Wilson; Guaraldi, Federica (Eds.) Endocrine Immunology (2017)

Most scientific reports debate the thymotropic and immuno-stimulating properties of the somato- trope growth hormone-releasing hormone (GHRH)/growth hormone (GH)/insulin-like growth factor (IGF)-1 axis ... [more ▼]

Most scientific reports debate the thymotropic and immuno-stimulating properties of the somato- trope growth hormone-releasing hormone (GHRH)/growth hormone (GH)/insulin-like growth factor (IGF)-1 axis, but there is still some disagreement about the physiological role of this axis in basal conditions. Moreover, some authors have hypothesized that the physiological role of the somato- trope axis only appears in stressful conditions (such as sepsis or infective and inflammatory diseases). This chapter will provide an extended overview of the expression of the components (signals and receptors) of the somatotrope axis and their properties on cells of the innate and adaptive immune system. It will also summarize some clinical studies suggesting a benefit for a short-term GH treat- ment in acute immunodeficiencies, and the importance of GH supplementation in adult GH defi- ciency. A new transgenic mouse model, the hypothalamic GHRH-deficient (Ghrh–/–) mouse, which exhibits a severe deficiency of the somatotrope axis, will be presented since it will be of great help in further deciphering the regulation by the GHRH/GH/IGF-1 axis on both immune development and function. Finally, we will discuss the implication of aging-related somatopause in relation to the general context of Immunosenescence. [less ▲]

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See detailLe choix du liégeois. Dîmègne de Louis Remacle
Baiwir, Esther ULg; Biquet, Stéphanie ULg

in Baiwir, Esther; Biquet, Stéphanie (Eds.) Petite anthologie subjective de littérature wallonne (2017)

Lecture et commentaire du seul poème en liégeois de Louis Remacle, "Dîmègne".

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See detailSampling only ten microliters of whole blood for the quantification of poorly soluble drugs: Itraconazole as case study
Thiry, Justine ULg; Evrard, Brigitte ULg; Nys, Gwenaël ULg et al

in Journal of Chromatography. A (2017), 1479

Nowadays in animal studies, it is important to comply with the so-called Three Rs rule by replacing or reducing the number of tested animals. Volumetric absorptive microsampling (VAMS) can be used to ... [more ▼]

Nowadays in animal studies, it is important to comply with the so-called Three Rs rule by replacing or reducing the number of tested animals. Volumetric absorptive microsampling (VAMS) can be used to collect small quantities (10 or 20 µL) of whole blood, thereby limiting the amount of animals needed. In this study, a quantitative method was developed and subsequently validated for the poorly soluble drug itraconazole (ITZ) using VAMS and ultra-high performance liquid chromatography (UHPLC) coupled to tandem mass spectrometry (MS). A proof of concept study showed that the optimized method is applicable to test the bioavailability of drug formulations containing ITZ. Using VAMS, smaller blood volumes can be taken per sampling point (10-20 µL instead of the conventional 0.2-0.5 mL) avoiding the sacrifice of animals. Moreover, the same rats can be used to compare different drug formulations which strengthens the validity of the results. In long-term bioavailability studies, it is necessary to guarantee the stability of the tested drugs supported on VAMS devices. In this study, we show that ITZ was only stable for 24 hours after collection with VAMS, but for at least two weeks by the storage of extracted samples at -80°C. [less ▲]

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See detailInteractive effects of ultraviolet radiation and thermal stress on two reef-building corals
Courtial, Lucile; Roberty, Stéphane ULg; Shick, Malcom et al

in Limnology and Oceanography (2017)

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See detailMolecular dynamics study of micelle proeprties according to their size
Lebecque, Simon ULg; Crowet, Jean-Marc ULg; Nasir, Mehmet Nail ULg et al

in Journal of Molecular Graphics and Modelling (2017)

Surfactants are molecules able to spontaneously self-assemble to form aggregates with well-defined properties, such as spherical micelles, planar bilayers, cylindrical micelles or vesicles. Micelles have ... [more ▼]

Surfactants are molecules able to spontaneously self-assemble to form aggregates with well-defined properties, such as spherical micelles, planar bilayers, cylindrical micelles or vesicles. Micelles have notably several applications in many domains, such as drug delivery or membrane protein solubilization. In this context, the study of micelle formation in relation with the structural and physico-chemical properties of surfactants is of great interest to better control their use in the different application fields. In this work, we use the MD approach developed by Yoshii et al. and extend it to surfactants with different structures. We aim to systematically investigate different micellar properties as a function of the aggregates size by a molecular dynamics approach, to get an insight into the micellar organization and to collect some relevant descriptors about micelle formation. For this, we perform short MD simulations of preformed micelles of various sizes and analyze three parameters for each micelle size, namely the eccentricity of the micelles, the hydrophobic/hydrophilic surface ratio and the hydrophobic tails hydration. If these parameters are known descriptors of micelles, they were not yet studied in this way by MD. We show that eccentricity, used as “validator” parameter, exhibits minimal values when the aggregate size is close to the experimental aggregation number for surfactants that are known to form spherical micelles. This hence indicates that our methodology gives consistent results. The evolution of the two descriptors follows another scheme, with a sharp increase and decrease, respectively, followed by a leveling-off. The aggregate sizes at which this stabilization starts to occur are close to the respective aggregation number of each surfactant. In our approach, we validate the use of these descriptors to follow micelle formation by MD, from “simple” surfactants to more complex structures, like lipopeptides. Our calculations also suggest that some peculiar behavior, like that of TPC, can be highlighted by our approach. In the context of peptidic surfactants, our methodology could further help to improve computer simulations combined to molecular thermodynamic models to predict micellar properties of those more complex amphiphilic molecules. [less ▲]

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See detailAssessing inter- and intramolecular continuous-flow strategies towards methylphenidate (Ritalin) hydrochloride
Gerardy, Romaric ULg; Winter, Marc; Vizza, Alessandra et al

in Reaction Chemistry and Engineering (2017)

The batch-to-flow translation of inter- and intramolecular strategies for the diastereoselective preparation of the active pharmaceutical ingredient threo-methylphenidate hydrochloride is presented. Both ... [more ▼]

The batch-to-flow translation of inter- and intramolecular strategies for the diastereoselective preparation of the active pharmaceutical ingredient threo-methylphenidate hydrochloride is presented. Both inter- and intramolecular strategies imply the telescoping of multiple processing steps and the generation of unstable diazo species under continuous-flow conditions. The intermolecular strategy relies on an unprecedented continuous-flow Rh-catalyzed intermolecular C–H carbene insertion, providing enriched threo-N-Boc methylphenidate in 38% or 19% isolated yield according to sequential or fully telescoped processes, respectively. Quantitative Boc-deprotection is carried out off-line. The intramolecular strategy relies on a continuous-flow thermal intramolecular C–H carbene insertion, providing enriched threo-methylphenidate hydrochloride in 70% isolated yield. A continuous-flow photochemical alternative is also presented. The critical step of the most promising intramolecular strategy is implemented on the mesoscale in a pilot-scale continuous-flow reactor. [less ▲]

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See detailTime course of familiarity and novelty decisions in aging
Delhaye, Emma ULg; Bastin, Christine ULg; Moulin, Christopher et al

Conference (2017)

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See detailOut of the ground: two coexisting fossorial toad species differ in their emergence and movement patterns
Székely, Diana ULg; Cogalniceanu, Dan; Székely, Paul et al

in Zoology (2017), 121

Understanding the way species with similar niches can coexist is a challenge in ecology.The niche partitioning hypothesis has received much support, positing that species can exploit available resources ... [more ▼]

Understanding the way species with similar niches can coexist is a challenge in ecology.The niche partitioning hypothesis has received much support, positing that species can exploit available resources in different ways. In the case of secretive species, behavioural mechanisms of partitioning are still poorly understood. This is especially true for fossorial frogs because individuals hide underground by day and are active only during the night. We investigated the nocturnal activity and tested the niche partitioning hypothesis in two syntopic fossorial spadefoot toads (Pelobates fuscus and P. syriacus) by examining interspecific variation in emergence from the soil. We employed a night vision recording system combined with video-tracking analyses in a replicated laboratory setting to quantify individual movement patterns, a procedure that has not been used until now to observe terrestrial amphibians. Most individuals appeared on the surface every night and returned to their original burrow (about 60% of the times), or dug a new one around morning. There was a large temporal overlap between the two species. However, P. syriacus was significantly more active than P. fuscus in terms of total distance covered and time spent moving, while P. fuscus individuals left their underground burrow more frequently than P. syriacus. Consequently, P. fuscus adopted more of a sit-and-wait behaviour compared to P. syriacus, and this could facilitate their coexistence. The use of night video-tracking technologies offered the advantage of individually tracking these secretive organisms during their nocturnal activity period and getting fine-grain data to understand their movement patterns. [less ▲]

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See detailSouche bactérienne génétiquement modifiée produisant de la kurstakine dans le milieu de culture
Lereclus, D.; Jacques, Philippe ULg; Gohar, M. et al

Patent (2017)

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See detailProteomic response of Macrobrachium rosenbergii hepatopancreas exposed to chlordecone: Identification of endocrine disruption biomarkers?
Lafontaine, Anne ULg; Baiwir, Dominique ULg; Joaquim-Justo, Célia ULg et al

in Ecotoxicology and Environmental Safety (2017)

The present work is the first study investigating the impacts of chlordecone, an organochlorine insecticide, on the proteome of the decapod crustacean Macrobrachium rosenbergii, by gel-free proteomic ... [more ▼]

The present work is the first study investigating the impacts of chlordecone, an organochlorine insecticide, on the proteome of the decapod crustacean Macrobrachium rosenbergii, by gel-free proteomic analysis of the hepatopancreas protein expression variations in organisms exposed to three environmental relevant concentrations of chlordecone (i.e. 0.2, 2 and 20 μg/L). Results revealed that 62 proteins were significantly up- or down-regulated in exposed prawns compared to controls. Most of these proteins are involved in important physiological processes such as ion transport, defense mechanisms and immune system, cytoskeleton dynamics, or protein synthesis and degradation. Moreover, it appears that 6% of the deregulated protein are involved in the endocrine system and in the hormonal control of reproduction or development processes of M. rosenbergii (e.g. vitellogenin, farnesoic acid omethyltransferase). These results indicate that chlordecone is potentially an endocrine disruptor compound for decapods, as already observed in vertebrates. These protein modifications could lead to disruptions of growth and reproduction of M. rosenbergii, and therefore of the fitness population on the long-term. Besides, these disrupted proteins could be suggested as biomarkers of exposure for endocrine disruptions in invertebrates. However, further investigations are needed to complete understanding of action mechanisms of chlordecone on proteome and endocrine system of crustaceans. [less ▲]

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See detailHigh-throughput strategies for the discovery and engineering of enzymes for biocatalysis
Jacques, Philippe ULg; Béchet, M.; Bigan, M. et al

in Bioprocess and Biosystems Engineering (2017), 40(2),

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See detailSpecular sets
Berthé, Valérie; De Felice, Clelia; Delecroix, Vincent et al

in Theoretical Computer Science (2017)

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See detailImpaired familiarity in individuals at risk for Alzheimer's disease: Commentary on Schoemaker et al. (2016)
Bastin, Christine ULg; Besson, Gabriel ULg

in Alzheimer's & Dementia: Diagnosis, Assessment & Disease Monitoring (2017), 6

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See detailL’IMAGE DU MOIS.Une tumeur cardiaque indéterminée
RADERMECKER, Régis ULg; RENSON, Virginie ULg; Delvenne, Philippe ULg et al

in Revue Médicale de Liège (2017), 72(1), 4-5

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