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See detailLe dosage du cobalt dans le sang par ICP-MS : application aux patients porteurs de prothèses de hanche en métal
MISTRETTA, Virginie ULg; Charlier, Corinne ULg

in Toxicologie Analytique & Clinique (2015), 27(2), 91-98

Objectif Les patients porteurs de prothèse de hanche à couple métal-métal (PHM) présentent un taux de cobalt circulant supérieur à celui de la population générale. Chez ces patients, le dosage du cobalt ... [more ▼]

Objectif Les patients porteurs de prothèse de hanche à couple métal-métal (PHM) présentent un taux de cobalt circulant supérieur à celui de la population générale. Chez ces patients, le dosage du cobalt permet d’apprécier le risque toxique éventuel de l’implant et peut servir d’indicateur de révision ou de remplacement de cette prothèse. A cette fin, une méthode de dosage a été validée et appliquée à des patients porteurs de PHM. Méthode Le dosage du cobalt est réalisé sur 500 μL de sang total, après dilution dix fois dans une solution aqueuse acide et après ajout de 100 μL d’une solution de standard interne titrée à 500 μg/L de germanium. L’échantillon est ensuite analysé par un spectromètre de masse avec source d’ionisation par plasma induit haute fréquence (ICP-MS). La méthode décrite a été validée par l’approche de l’erreur totale au moyen du logiciel e•noval (Arlenda®). Résultats La méthode de dosage a été validée avec succès. Elle est linéaire de 0,5 à 50 μg/L. L’inexactitude et l’imprécision intra- et inter-essais sont inférieures à 5 % et 8 %, respectivement. L’incertitude de mesure est inférieure à 17 %. La concentration sanguine moyenne de cobalt estimée sur une période de 1 an à partir de 107 prélèvements de patients porteurs de PHM est de 2,61 μg/L (nombre de patients : 98 ; âge moyen : 60 ans ; ratio hommes/femmes : 38/60). Ce résultat est inférieur au seuil recommandé dans ces populations, fixé à 7 μg/L de cobalt sanguin. Conclusion La méthode analytique présentée permet de doser le cobalt sanguin, notamment chez les patients porteurs de PHM. Ce dosage est recommandé pour le suivi de ces patients, en association avec un examen clinique et radiologique. [less ▲]

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See detailFavoriser l'émergence d'innovations institutionnelles dans la gouvernance des universités du Moyen-Orient: retour sur notre rôle de chercheur-intervenant dans un projet de collaboration internationale
Lisein, Olivier ULg; Pichault, François ULg

Conference (2015, June)

Cette communication revient sur un projet de recherche-action – visant à mettre en œuvre de nouveaux modes de gouvernance au sein de cinq universités d’Afrique méditerranéenne et du Moyen-Orient – que ... [more ▼]

Cette communication revient sur un projet de recherche-action – visant à mettre en œuvre de nouveaux modes de gouvernance au sein de cinq universités d’Afrique méditerranéenne et du Moyen-Orient – que nous avons géré pendant trois ans. A partir des enseignements que nous avons retirés de nos responsabilités dans ce projet collaboratif, nous proposons un regard réflexif sur notre position et notre rôle de chercheurs-intervenants invités à gérer un projet de recherche-action dans des contextes culturels auxquels nous n’étions pas habitués, avec des méthodologies implicatives pas nécessairement partagées par toutes les parties. Nous élaborons sur cette base différentes pistes d’explication potentielle aux difficultés méthodologiques que nous avons rencontrées et aux résultats contrastés que nous avons atteints. [less ▲]

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See detailThe Authority of Strasbourg Jurisprudence from the Perspective of the International Criminal Court
Deprez, Christophe ULg

in Journal Européen des Droits de l'Homme = European Journal of Human Rights (2015), 3

Although the case law of the European Court of Human Rights (Eur. Ct. H.R.) exerts a critical influence on the development of the practice of the International Criminal Court (ICC), it remains unclear ... [more ▼]

Although the case law of the European Court of Human Rights (Eur. Ct. H.R.) exerts a critical influence on the development of the practice of the International Criminal Court (ICC), it remains unclear what the exact authority of such external jurisprudence is vis-à-vis the international criminal judge. This article addresses the question whether ICC judges have used Strasbourg jurisprudence in a consistent manner, in particular as regards the authority which they decided to vest the latter with. It demonstrates that the Court’s chambers have relied on European human rights practice, alternatively, as a mere source of inspiration, as binding interpretation, as a corpus having ambivalent authority, or as a source whose value it simply left unspecified. It therefore concludes that the ICC has been mostly inconsistent regarding the authority of the case law of the Eur. Ct. H.R. in the context of international criminal proceedings. Such inconsistency, it is argued, amounts to a discretionary use of Strasbourg jurisprudence that further reinforces the ICC’s paradoxical position as the ultimate authority for defining the boundaries of its own human rights constraint. [less ▲]

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See detailExtracellular peptidase hunting for improvement of protein production in plant cells and roots
Lallemand, Jérôme ULg; Bouché, Frédéric ULg; Desiron, Carole et al

Poster (2015, June)

Plant-based biopharmaceuticals have gained a lot of interest in the past decade due to their reduced cost and relative safety compared to mammalian cell cultures. While the first plant-made recombinant ... [more ▼]

Plant-based biopharmaceuticals have gained a lot of interest in the past decade due to their reduced cost and relative safety compared to mammalian cell cultures. While the first plant-made recombinant proteins are now reaching the market, the production systems still need improvements to maximize their competitiveness, proteolysis being one of the main factors limiting the yields. Identifying and inhibiting in vivo endogenous proteases involved in the degradation of recombinant proteins could then lead to a significant increase in production yields. In this study, we focused on two different production systems in Arabidopsis thaliana: rhizosecretion and cell suspensions. Extracellular proteases of both systems were used in vitro to study the conditions of target protein degradation (Bovine Serum Albumine, BSA). First, proteases from both systems degrade BSA at both acidic and neutral-to-basic pH conditions. Then, serine and metallopeptidases were shown to be the main protease classes responsible for BSA degradation by rhizosecreted proteomes or extracellular cell culture media, respectively. Finally, the biochemical tests were coupled to a bioinformatics analysis of publicly available transcriptomic data, in order to reduce the number of the proteases most likely involved in BSA degradation. Using this method, only five serine proteases and two metallopeptidases remain candidates for an amiRNA-mediated in vivo inhibition. [less ▲]

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See detailDe novo sequencing using MELD proteolysis coupled to a "sequence assembly" algorithm
Mazzucchelli, Gabriel ULg; Zimmerman, Tyler; Smargiasso, Nicolas ULg et al

in 63rd ASMS Conference Proceedings, May 30 - June 4 2015, St. Louis, MO (2015, June)

Introduction Protein de novo sequencing requires a method that combines extensive MSMS fragmentation and an appropriate data processing. This can be applied either on intact protein or on its proteolytic ... [more ▼]

Introduction Protein de novo sequencing requires a method that combines extensive MSMS fragmentation and an appropriate data processing. This can be applied either on intact protein or on its proteolytic peptides. Peptide analysis has the advantage to be compatible with well-known workflows. Nevertheless the connectivity between peptides is lost. Taking these considerations into account, a specific digestion method and a sequence assembly software were developed. The MELD method relies on a combination of MultiEnzymatic AND Limited proteolytic Digestions. The MELD generates in a single experiment numerous different peptides with miss-cleavages that overlap when aligned on the matching protein sequence. The two major benefices are an increased probability to obtain the entire protein sequence and a redundancy in the protein sequence matches. Methods The MELD consists in two parallel 2h digestions both using an optimized protease mixture. The mixtures are composed of the same proteases but in different relative quantities. Analyses were performed by UPLC-Orbitrap (IClass, Waters, QExactive, Thermo). Data were processed with PEAKS (BSI) to generate de novo sequence candidates. After data importation into our software, a seed sequence is set or can be found automatically. The software extends the seed sequence in both directions with moving windows of three amino acids, plus a fourth one to be added. The added amino acid is validated in several ways, including by the total frequency of occurrence, by larger windows of aa, by the local spectrum-derived confidence, and by combinations of these factors. Preliminary Data The MELD protocol was first validated by applying a traditional database search workflow on several commercial proteins with an inter-day and inter-individual procedure. These experiments showed 100% sequence coverage for each protein analyzed, involving information on peptide identity and modifications localization. Strong confident identification was obtained due to multiple overlapping peptides matches with the given sequence and with a high number of overlapping peptides assignments. The analysis of the four proteins provided the following results: HSA, Myoglobin and Lysozyme were identified with 100% sequence coverage with respectively 890, 300 and 120 unique peptides (CV<10%, peptide FDR<0.1%). The variable region of each heavy and light chains of Adalimumab antibody were identified with 100% sequence coverage. With the MELD, an average of 10 different peptides covering each sequence stretch of the protein could be obtained. The combinatory effect of the multiple enzymes used and the limited digestions leads to an increased robustness, very high confidence identifications and allows clear localization of PTMs. The MELD protocol as presented here and tested on several pure proteins digested in solution, certainly improves the general "bottom-up" strategy applied for highly confident protein identification and would allow better protein characterization, even for those having PTMs. In addition, in our analysis, each fragment position of the entire protein sequence was evidenced either by a "y" or a "b" fragment ion. This high and confident amount of information enables extensive de novo sequencing using PEAKS software, followed by application of our “sequence assembly” algorithm. The first version of our assembling tool on MELD experimental data generated long sequence tags, up to 90 amino acids long. [less ▲]

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See detailPanorama des incitants fiscaux destinés à stimuler l’investissement privé dans le secteur cinématographique
Vandenbulke, Antoine ULg

in Agefi Luxembourg (2015)

The film industry in Europe is largely funded through States support mechanisms. Among the many instruments set up by the various national laws, particular attention should be given to tax incentives ... [more ▼]

The film industry in Europe is largely funded through States support mechanisms. Among the many instruments set up by the various national laws, particular attention should be given to tax incentives aimed to stimulate private capital investments in this sector particularly risky. This short paper aims to outline the main features of tax breaks for the private financing of an audiovisual work in three States in particular: France, Belgium and Luxembourg. [less ▲]

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See detailCROSSROADS le magazine sonore des musiques africaines-américaines
Sacré, Robert ULg

Diverse speeche and writing (2015)

Magazine sonore des musiques africaines-américaines - - - - - page Facebook www.facebook.com/robert.sacre.9 --------- podcast www.mixcloud.com/robertsacre9

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See detailLe financement des entités décentralisées dans la nouvelle Belgique fédérale
Bayenet, Benoît ULg; Husson, Jean-François ULg

in La dotation globale de fonctionnement en question, éléments d'évaluation et perspectives : contributions pour un débat d'actualité (2015)

This publication debates on the current general grant scheme to French municipalities. Our contribution brings a comparison with the Belgian schemes for financing sub-national entities (Regions ... [more ▼]

This publication debates on the current general grant scheme to French municipalities. Our contribution brings a comparison with the Belgian schemes for financing sub-national entities (Regions/Communities and local government). [less ▲]

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See detailMélanges en l'honneur de Roger Marijnissen
Verbeeck, Muriel ULg

Book published by CeROArt (2015)

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See detail“Elements of the Sublime (and the Grotesque) in Crime Fiction”
Dechene, Antoine ULg

Conference (2015, June)

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See detailProceedings of the 12th Workshop on Models and Algorithms for Planning and Scheduling Problems
Marchetti-Spaccamela, Alberto; Crama, Yves ULg; Goossens, Dries et al

Book published by KU Leuven (2015)

This volume contains abstracts of talks presented at the 12th Workshop on Models and Algorithms for Planning and Scheduling Problems (MAPSP 2015), held from June 8 to June 12, 2015, in La Roche-en-Ardenne ... [more ▼]

This volume contains abstracts of talks presented at the 12th Workshop on Models and Algorithms for Planning and Scheduling Problems (MAPSP 2015), held from June 8 to June 12, 2015, in La Roche-en-Ardenne, Belgium. MAPSP is a biennial workshop dedicated to all theoretical and practical aspects of scheduling, planning, and timetabling. The abstracts in this volume include 5 invited talks by Onno Boxma, Michel Goemans, Willem-Jan van Hoeve, Rolf Niedermeier, and Stephan Westphal, plus 88 contributed talks. [less ▲]

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See detailThe ICE protocol for ecological continuity: a new tool to evaluate the upstream fish passage success at physical barriers
Ovidio, Michaël ULg; Larinier, Michel; Burgun, Vincent et al

Conference (2015, June)

The recording and classification of obstacles that can interfere with the movements and migrations of fish is critical information to plan river restoration program. To date a major problem is to ... [more ▼]

The recording and classification of obstacles that can interfere with the movements and migrations of fish is critical information to plan river restoration program. To date a major problem is to determine the potential effect of each obstacle and to select the problematic sites that should be improved to restore longitudinal connectivity (construction of fish passage facilities, removal or modification of the obstacles). Fish passage success about an obstacle is complex and depends on the hydraulic conditions over and at the foot of the obstacle in relation to swimming and leaping capabilities of the fish species concerned. This selection and classification is too often biased because managers lack information’s on the fish capabilities to leap physical obstacles. The need for a simple, reliable and standardised assessment method for use by a wide range of environmental stakeholders rapidly became evident. We responded to the challenge and developed the ICE protocol as the basis for the required ecological-continuity assessment method, particularly in the context of the European Water Framework Directive implementation. The ICE protocol coordinated by ONEMA is based on an integration of the topographical and hydraulic characteristics of obstacles with the physical capabilities (swimming, jumping or crawling) of the fish species analysed. It requires the gathering of standardised descriptive variables on each obstacle. The result of the analysis is an indication on the risks of a structure constituting a more or less severe obstacle (5 classes) for a given fish species (n=47) or group of species. Particular attention was paid to the practical aspects of the method (time required and necessary human resources) to facilitate its use in a wide variety of situations and areas. Each procedure is presented as a flow chart to assist in decision-making, thus making the protocol easy to use for a wide range of people. [less ▲]

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See detailAssessing the passage of obstacles by fish. Concepts, design and application
Baudoin, Jean-Marc; Burgun, Vincent; Chanseau, Matthieu et al

Book published by Onema - Editeur: Véronique Barre (2015)

The fragmentation of habitats has been acknowledged for 30 years as one of the five main factors of biodiversity loss, in conjunction with pollution, overuse of natural resources, invasive species and ... [more ▼]

The fragmentation of habitats has been acknowledged for 30 years as one of the five main factors of biodiversity loss, in conjunction with pollution, overuse of natural resources, invasive species and climate change. Since then, public environmental policies have strived to restore the connectivity of natural habitats. That is particularly the case for water policy, which has made the ecological continuity of rivers a central element in policy planning, a quality element for monitoring programmes and one of the basic guidelines for river-basin management plans (RBMP). The time has thus come for decisions, with the resulting controversy given that any attempt to modify existing discontinuities impacts our perception of landscapes and various uses of aquatic environments. Given that there is, on average, at least one obstacle for every five kilometres of river in continental France, this issue concerns the entire country, its population and all water managers. That explains why it was necessary to have a single set of standardised procedures for selecting the work to be done on the basis of objective and comparable data. For the ecological continuity of fish, we now have the ICE method presented in this book, which describes obstacles in rivers and assesses the capacity of fish to overcome those obstacles during their upstream migration. The design, development and national deployment of this method required five years of intense, collective effort on the part of numerous scientists and the Onema local and regional offices. The method is the result of an outstanding multi-disciplinary approach involving both hydraulics and ecology, two disciplines that some people might see as irreconcilable, but that must work together synergistically in the effort to restore environments. The publication of this book in the Knowledge for action series marks the transition from the team that developed the method to the people who will use it to acquire better understanding of ecological continuity in rivers and as an operational tool in implementing water policy and enhancing biodiversity. [less ▲]

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See detailOn n'est pas à une seconde près, si?
Van Der Beken, Emeline ULg

Article for general public (2015)

L'année 2015 comptera une seconde supplémentaire. Le passage du 30 juin 2015 au 1 juillet 2015 suivra la séquence temporelle suivante: 23 h 59 min 59 s, 23 h 59 min 60 s, 0 h 0 min 0 s. L'ajout de cette ... [more ▼]

L'année 2015 comptera une seconde supplémentaire. Le passage du 30 juin 2015 au 1 juillet 2015 suivra la séquence temporelle suivante: 23 h 59 min 59 s, 23 h 59 min 60 s, 0 h 0 min 0 s. L'ajout de cette seconde, dite seconde intercalaire, a pour objectif de conserver le temps légal en synchronisation avec le mouvement de rotation de la Terre sur elle-même. [less ▲]

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See detailA First Look at the Nonadiabatic Properties of Pulsating Accreting White Dwarfs of the GW Lib Type
Van Grootel, Valérie ULg; Fontaine, G.; Brassard, P. et al

in Astronomical Society of the Pacific Conference Series (2015, June), 493

We present results of a detailed stability survey of the pulsation properties of accreting white dwarfs of the GW Lib type. This is based on several state-of-the- art white dwarf evolutionary sequences ... [more ▼]

We present results of a detailed stability survey of the pulsation properties of accreting white dwarfs of the GW Lib type. This is based on several state-of-the- art white dwarf evolutionary sequences with varying envelope compositions, from pure hydrogen to pure helium. Using the same tools as in Van Grootel et al. (2013), where we have presented the first consistent view of the ZZ Ceti instability strip, we have mapped the GW Lib instability strip over the effective temperature-surface gravity plane, and as a function of envelope composition. We find that the location of the GW Lib instability domain is a strong and continuous function of the assumed envelope composition. We can accomodate all of the known GW Lib pulsators in various strips according to their atmospheric compositions. [less ▲]

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