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See detailImpact du séchage sur la viabilité de Pseudomonas fluorescens (synthèse bibliographique)
Mputu Kanyinda, Jean-Noël ULg

in Biotechnologie, Agronomie, Société et Environnement = Biotechnology, Agronomy, Society and Environment [=BASE] (2014), 18(1), 134-141

The drying Pseudomonas fluorescens makes more economical storage, transportation and marketing. It aims to stop and to stabilize all biological activities for an optimal storage, compatible with the ... [more ▼]

The drying Pseudomonas fluorescens makes more economical storage, transportation and marketing. It aims to stop and to stabilize all biological activities for an optimal storage, compatible with the conservation of maximum viability of microorganisms desired. The viability of bacteria after drying depends on the operating conditions of the latter. One of the most important criteria to consider during the drying of biologically active products is the quality of the final dried product. Freeze-drying is the most drying method used for Pseudomonas. But temperature changes it induced are not without consequence for the cells. They are responsible for cell damage (peroxidation of fatty acids) and genetic (proteins and DNA oxidation). However, use of protective compounds during freeze-drying and during storage increases significantly the rate of cell viability. [less ▲]

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See detailInfluence du changement climatique sur l'accroissement du hêtre en Wallonie
Latte, Nicolas ULg; Claessens, Hugues ULg

in Forêt Wallonne (2014), (129), 46-47

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See detailQuantification of HTLV-1 Clonality and TCR Diversity.
Laydon, Daniel J.; Melamed, Anat; Sim, Aaron et al

in PLoS computational biology (2014), 10(6), 1003646

Estimation of immunological and microbiological diversity is vital to our understanding of infection and the immune response. For instance, what is the diversity of the T cell repertoire? These questions ... [more ▼]

Estimation of immunological and microbiological diversity is vital to our understanding of infection and the immune response. For instance, what is the diversity of the T cell repertoire? These questions are partially addressed by high-throughput sequencing techniques that enable identification of immunological and microbiological "species" in a sample. Estimators of the number of unseen species are needed to estimate population diversity from sample diversity. Here we test five widely used non-parametric estimators, and develop and validate a novel method, DivE, to estimate species richness and distribution. We used three independent datasets: (i) viral populations from subjects infected with human T-lymphotropic virus type 1; (ii) T cell antigen receptor clonotype repertoires; and (iii) microbial data from infant faecal samples. When applied to datasets with rarefaction curves that did not plateau, existing estimators systematically increased with sample size. In contrast, DivE consistently and accurately estimated diversity for all datasets. We identify conditions that limit the application of DivE. We also show that DivE can be used to accurately estimate the underlying population frequency distribution. We have developed a novel method that is significantly more accurate than commonly used biodiversity estimators in microbiological and immunological populations. [less ▲]

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See detailRésumé CRAIE, 18 février 2014, recours n°645, Savary
Delnoy, Michel ULg

in Aménagement - Environnement (2014), (3), 190

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See detailRésumé C.E.D.H., 28 novembre 2013, Österreichische vereinigung zur erhaltung et crts contre Autriche
Delnoy, Michel ULg

in Aménagement - Environnement (2014), (2), 97

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See detailRésumé C.E., n°227.230, du 29 avril 2014, Lenain et Gatto
Delnoy, Michel ULg

in Aménagement - Environnement (2014), (5), 257

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See detailInsight into the development of a carbonate platform through a multi-disciplinary approach - A case study from the Upper Devonian slope deposits of Mount Freikofel (Carnic Alps, Austria/Italy)
Pas, Damien ULg; Da Silva, Anne-Christine ULg; Suttner, Thomas et al

in International Journal of Earth Sciences (2014), 103

The development and behavior of Million year-scaled depositional sequences recorded within Palaeozoic carbonate platform has remained poorly examined. Therefore, the understanding of palaeoenvironmental ... [more ▼]

The development and behavior of Million year-scaled depositional sequences recorded within Palaeozoic carbonate platform has remained poorly examined. Therefore, the understanding of palaeoenvironmental changes that occur in geological past is still limited. We herein undertake a multi-disciplinary approach (sedimentology, conodont biostratigraphy, magnetic susceptibility and geochemistry) of a long-term succession in the Carnic Alps which offers new insights into the peculiar evolution of one of the best example of Palaeozoic carbonate platform in Europe. The Freikofel section, located in the central part of the Carnic Alps represents an outstanding succession in a fore-reef setting, extending from the latest Givetian (indet. falsiovalis conodont Zones) to the early Famennian (Lower crepida conodont Zone). Sedimentological analysis allowed to propose a sedimentary model dominated by distal slope and fore-reef slope deposits. The most distal setting is characterized by an autochthonous pelagic sedimentation showing local occurrence of thin-bedded turbiditic deposits. In the fore-reef slope, in a more proximal setting, there is an accumulation of various autochthonous and allochthonous fine- to coarse-grained sediments originated from the interplay of gravity-flow currents derived from the shallow-water and deeper-water area. The temporal evolution of microfacies in the Freikofel section evolves in two main steps corresponding to the Freikofel (Unit 1) and the Pal (Unit 2) Limestones. Distal slope to fore-reef lithologies and associate changes are from base to top of the section: (U1) thick bedded litho- and bioclastic breccia beds with local fining upward sequence and fine-grained mudstone intercalations corresponding, in the fore-reef setting, to the dismantlement of the Eifelian – Frasnian carbonate platform during the early to late Frasnian time (falsiovalis to rhenana superzones) with one of the causes being the Late Givetian major rift pulse; (U2) occurrence of thin-bedded red nodular and cephalopod-bearing limestones with local lithoclastic grainstone intercalations corresponding to a significant deepening of the area and the progressive withdrawal of sedimentary influxes toward the basin, in relation with late Frasnian sea-level rise. Magnetic susceptibility and geochemical analyses were also performed along the Freikofel section and demonstrate the inherent-parallel link existing between variation in magnetic susceptibility values and proxy for terrestrial input. Interpretation of magnetic susceptibility in term of palaeoenvironmental processes reflect that even though distality remains the major parameter influencing magnetic susceptibility values, carbonate production and water agitation also play an important role. [less ▲]

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See detailMeteorological variation in travel behaviour
Creemers, Lieve; Wets, Geert; Cools, Mario ULg

Conference (2014)

Weather causes a variety of impacts on the transportation system. This paper contributes to the weather-related transport literature by investigating the meteorological variation in revealed preference ... [more ▼]

Weather causes a variety of impacts on the transportation system. This paper contributes to the weather-related transport literature by investigating the meteorological variation in revealed preference travel data. The main objective of this paper is to investigate the impact of weather conditions on revealed activity participation (trip motives) and revealed modal choices in the Netherlands. To this end, data from the Dutch national travel household survey 2008 was matched to hourly weather data provided by the Royal Dutch Meteorological Institute. Two GEE-MNL models are constructed, namely one for modelling the impact of weather conditions on trip motive and one to assess the effect on modal choice. The parameter estimates of the weather variables indicate that, depending on which travel attribute one focuses, other factors might play a role. Nonetheless, fog, sunshine duration and temperature have a significant impact in both models. Unexpected is the fact that snow and ice cover do not play a role at all. Nonetheless, this finding can be accounted for by the relative low occurrence of these weather types in the study area. It is important to integrate these identified impacts of weather in travel demand modelling frameworks, since this will help to achieve a higher accuracy and more realistic traffic forecasts. This allows policy makers to make better long-term and short-term decisions to achieve various political goals, such as the development towards a sustainable transportation system. Further research in this regard, should emphasize on the role of weather conditions and activity-scheduling attributes. [less ▲]

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See detailRésumé C.E., n°225.793, du 12 décembre 2013, Commune de Braine-l’Alleud
Delnoy, Michel ULg

in Aménagement - Environnement (2014), (3), 176

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See detailInterstitial insulin kinetic parameters for a 2-compartment insulin model with saturable clearance
Pretty, Christopher G.; Le Compte, Aaron; Penning, Sophie ULg et al

in Computer Methods & Programs in Biomedicine (2014)

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See detailEvidence-based practice: fondements et réflexions sur l’apport en clinique.
Maillart, Christelle ULg; Durieux, Nancy ULg

in Langage et Pratiques (2014), 53

L’evidence-based practice ou pratique basée sur les preuves est une approche de plus en plus encouragée en logopédie au niveau international. Cet article présente les principaux composants en détaillant ... [more ▼]

L’evidence-based practice ou pratique basée sur les preuves est une approche de plus en plus encouragée en logopédie au niveau international. Cet article présente les principaux composants en détaillant la nature des preuves liées aux trois piliers de l’EBP (preuves externes, preuves internes ou préférences du patient). La démarche de l’EBP est brièvement discutée ainsi que les freins et les bénéfices attendus en clinique. [less ▲]

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See detailWeighing Elephants with Kitchen Scales. The relevance of traditional performance measurement tools for social enterprises
Mouchamps, Hugues ULg

in International Journal of Productivity & Performance Management (2014), 63(6), 727-745

Purpose – The purpose of this paper is to analyze whether the use of current performance tools is consistent with the specific features of social enterprises. Design/methodology/approach – In a first ... [more ▼]

Purpose – The purpose of this paper is to analyze whether the use of current performance tools is consistent with the specific features of social enterprises. Design/methodology/approach – In a first phase, the main performance tools are divided into strategic planning tools, reporting tools and economic optimization techniques. In a second phase, 15 criteria emerge from a literature review to characterize the specific features of social enterprises. These criteria are brought together into an analytical framework, which makes it possible to analyze the relevance of each performance tool in relation to the specific features of social enterprises. Findings – What comes out of the analysis is that the tools globally fail to account for the specific features of social enterprises. That none of them met more than half of the criteria suggests the need for new performance tools based on strong theoretical bases. [less ▲]

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See detailOrigin and evolution of the early land plants in Argentina and South America through the palynological record
Rubinstein, C.V.; Garcia Muro, V.J.; Steemans, Philippe ULg

Conference (2014)

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See detailStrategies for Using the Sheep Ovarian Cortex as a Model in Reproductive Medicine
Fransolet, Maïté ULg; Labied, Soraya ULg; Henry, Laurie ULg et al

in PLoS ONE (2014), 9(3), 91073

Objective: To evaluate and compare the distribution and density of primordial follicles within a whole sheep ovary and to gain insight into how to overcome the impact of natural follicular heterogeneity ... [more ▼]

Objective: To evaluate and compare the distribution and density of primordial follicles within a whole sheep ovary and to gain insight into how to overcome the impact of natural follicular heterogeneity on the experimental results. Design: Histological study. Setting: Academic research center. Animals: Five- to nine-month-old ewes. Interventions: Freshly sampled whole sheep ovaries were collected and prepared for histological analysis. Main Outcome Measure(s): The follicular densities and distributions were determined for hematoxylin and eosin sections. A mathematical model was derived based on the follicle counts and Monte-Carlo simulations. Results: Heterogeneous distributions and densities of primordial follicles were identified 1) for distinct areas of the same ovarian cortex, 2) between the ovaries of the same animal and 3) across different ewes. A mathematical model based on the analysis of 37,153 primordial follicles from 8 different ovaries facilitated the estimation of the number of cortical biopsies and sections that had to be analyzed to overcome such heterogeneity. Conclusion: The influence of physiological follicular heterogeneity on experimental and clinical results can be overcome when a definite number of cortical pieces and sections are taken into consideration. [less ▲]

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See detailEfficient estimation of the high-order response statistics of a wind-excited oscillator with nonlinear velocity feedback
Denoël, Vincent ULg; Carassale, Luigi

in Proceedings of the XIII Conference of the Italian Association for Wind engineering (2014)

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See detailEmbryonic Protein Undernutrition by Albumen Removal Programs the Hepatic Amino Acid and Glucose Metabolism during the Perinatal Period in an Avian Model.
Willems, Els; Hu, Tjing-Tjing; Soler Vasco, Laura et al

in PloS one (2014), 9(4), 94902

Different animal models have been used to study the effects of prenatal protein undernutrition and the mechanisms by which these occur. In mammals, the maternal diet is manipulated, exerting both direct ... [more ▼]

Different animal models have been used to study the effects of prenatal protein undernutrition and the mechanisms by which these occur. In mammals, the maternal diet is manipulated, exerting both direct nutritional and indirect hormonal effects. Chicken embryos develop independent from the hen in the egg. Therefore, in the chicken, the direct effects of protein deficiency by albumen removal early during incubation can be examined. Prenatal protein undernutrition was established in layer-type eggs by the partial replacement of albumen by saline at embryonic day 1 (albumen-deprived group), compared to a mock-treated sham and a non-treated control group. At hatch, survival of the albumen-deprived group was lower compared to the control and sham group due to increased early mortality by the manipulation. No treatment differences in yolk-free body weight or yolk weight could be detected. The water content of the yolk was reduced, whereas the water content of the carcass was increased in the albumen-deprived group, compared to the control group, indicating less uptake of nutrients from the yolk. At embryonic day 16, 20 and at hatch, plasma triiodothyronine (T3), corticosterone, lactate or glucose concentrations and hepatic glycogen content were not affected by treatment. At embryonic day 20, the plasma thyroxine (T4) concentrations of the albumen-deprived embryos was reduced compared to the control group, indicating a decreased metabolic rate. Screening for differential protein expression in the liver at hatch using two-dimensional difference gel electrophoresis revealed not only changed abundance of proteins important for amino acid metabolism, but also of enzymes related to energy and glucose metabolism. Interestingly, GLUT1, a glucose transporter, and PCK2 and FBP1, two out of three regulatory enzymes of the gluconeogenesis were dysregulated. No parallel differences in gene expressions causing the differences in protein abundance could be detected pointing to post-transcriptional or post-translational regulation of the observed differences. [less ▲]

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See detailRecommadantions européennes concernant la thérapie de resynchronisation cardiaque
ROBINET, Sébastien ULg; DELCOUR, Alexandre ULg; Lancellotti, Patrizio ULg

in Revue Médicale de Liège (2014), 69(4), 180-187

Cardiac resynchronization therapy (CRT) is one of the most successful heart failure therapies to emerge in the last 25 years; it is applicable to > 25-30% of patients with symptomatic heart failure. In ... [more ▼]

Cardiac resynchronization therapy (CRT) is one of the most successful heart failure therapies to emerge in the last 25 years; it is applicable to > 25-30% of patients with symptomatic heart failure. In this article, we summarize the recommendations of the European Society of Cardiology of 2013 on cardiac resynchronization therapy. [less ▲]

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