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See detailMyoferlin: an indispensable component in VEGFA secretion by pancreas cancer cells
Fahmy, Karim ULg; Peulen, Olivier ULg; Castronovo, Vincenzo ULg et al

Poster (2015, January 31)

In this poster, our laboratory showed the importance of myoferlin, a biomarker of pancreas cancer, in the controle of VEGF-A mediated angiogenesis. Our laboratory showed that silencing myoferlin in ... [more ▼]

In this poster, our laboratory showed the importance of myoferlin, a biomarker of pancreas cancer, in the controle of VEGF-A mediated angiogenesis. Our laboratory showed that silencing myoferlin in pancreas cancer cells, BxPC-3, provoques a decrease in cell prolifération in vitro and a decrease in tumor volumes in animal model. Myoferlin silencing also provokes a decrease in VEGF-A secretion in the conditioned medium and that decrease was abserved in the animal model as a decrease in microvessels dencity. It appeared that this decrease in secretion is due to a a blockage in the exocytosis. Our data also showed a significate correlation between myoferlin expression and microvessels density in patients section. [less ▲]

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See detailMyoferlin Regulates Endosomal Trafficking and Tunes Cancer Cell Metabolism
Blomme, Arnaud; Costanza, Brunella; Thiry, Marc ULg et al

Poster (2015, January 31)

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See detailWildflower strips for crop protection: What do we know ? What should we know ?
Hatt, Séverin ULg; Uyttenbroeck, Roel ULg; Bodson, Bernard ULg et al

Poster (2015, January 30)

Wildflower strips (WFS) are known to support the conservation of a large diversity of insects and thus natural enemies (i.e. predators and parasitoids) that can control pests. However, the conclusions of ... [more ▼]

Wildflower strips (WFS) are known to support the conservation of a large diversity of insects and thus natural enemies (i.e. predators and parasitoids) that can control pests. However, the conclusions of studies looking at the efficiency of WFS to control pests are not unanimous. Indeed, the enhancement of pest control seems to depend on (1) the ability of flowers to attract the natural enemies at the right moment and (2) the capacity of natural enemies to migrate into the adjacent crops to attack pests. Therefore, constituting appropriate flower mixes may be an essential lever to enhance the efficiency of pest control. In this context, using functional diversity is promising. To our knowledge, few studies have tested the impact of the functional diversity of a flower mix on insect abundance and diversity and the control of pests. Through this contribution, the insect diversity and abundance found to be associated with the different kinds of WFS and management applied will be discussed, as well as the further research needed. [less ▲]

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See detailStudy of durability and the physico-mechanical properties of thermally modified wood
Ninane, Maxime; Jourez, Benoît ULg; Hebert, Jacques ULg

Poster (2015, January 30)

Heat-treated wood is a credible alternative for certain kinds of tropical timber. It is also an alternative for certain polluting wood preservatives. This study aims to determine the changes in physico ... [more ▼]

Heat-treated wood is a credible alternative for certain kinds of tropical timber. It is also an alternative for certain polluting wood preservatives. This study aims to determine the changes in physico-mechanical properties and durability of wood that is subjected to heat treatment. For this, we studied five species (oak, ash, natural beech and a variant known as steamed beech, poplar, Douglas-fir). For each species, we had fifteen reference samples and fifteen corresponding treated samples. The results show a decrease in the hygroscopicity and an increase in dimensional stability of heat-treated wood in relation to the degradation of hemicelluloses. The mechanical properties are influence variously, because heat-treated wood is stiffer but also more brittle. The modulus of elasticity increases slightly after treatment. Side hardness and compressive fracture of heat-treated wood may increase up to 30%. On the other hand, the modulus of rupture, the ability to slit and impact may decrease by 50%, 60% and 70%. The durability of heat-treated wood against wood-destroying fungi increases. However, within each species, the durability increase varies greatly. Additional tests conducted to understand this variability, suggests that the heat distribution is not homogeneous within the processing unit. Moreover, our work suggests that durability is related to the fixed carbon content and that this path would be interesting to explore in the future. [less ▲]

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See detailL'arme de la transition énergétique pour combattre les djihadistes
Ernst, Damien ULg; Hermans, Michel ULg

Article for general public (2015)

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See detailLangmuir film balance- A technique to study the interaction of β-cyclodextrin with cholesterol in milk fat globule membrane monolayers
Malik, Priyanka ULg; Danthine, Sabine ULg; Paul, Aman ULg et al

Poster (2015, January 30)

Milk is a natural complex system present as oil in water emulsion. The natural organisation of milk fat globules is stabilized by the presence of a natural membrane, called the milk fat globule membrane ... [more ▼]

Milk is a natural complex system present as oil in water emulsion. The natural organisation of milk fat globules is stabilized by the presence of a natural membrane, called the milk fat globule membrane (MFGM). It has tripartite structure consisting of a monolayer facing the inner triacylglycerol core and an external bilayer. This MFGM accounts for 2-6% of fat globule. The MFGM is composed of both polar lipids and proteins. Cholesterol is also present in MFGM and accounts for about 2% of MFGM. Langmuir film balance is a technique to study monolayers and is a useful tool to examine the effect of cholesterol removal on surface properties of MFGM. β-cyclodextrin has no surface activity of its own and is thus a useful tool to study the effect of cholesterol desorption on surface properties in membrane. The removal of monolayer cholesterol to the sub-phase is proportional to the area decrease of the monolayer at a constant surface pressure. Amount of cholesterol removed can also be calculated as a function of mean molecular area at a given pressure, time and temperature. Using the monolayer technique, it is possible to study the interactions between β-cyclodextrin and MFGM film as well as effect of cholesterol removal in altering the surface properties MFGM. [less ▲]

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See detailUtility of removing cholesterol from an industrial by-product- Buttermilk powder
Malik, Priyanka ULg; Danthine, Sabine ULg; Paul, Aman ULg et al

Poster (2015, January 30)

Almost 16% of milk produced in European Union is being converted to butter. Buttermilk, is a low cost by-product from butter manufacture and available in large quantities but has been considered ... [more ▼]

Almost 16% of milk produced in European Union is being converted to butter. Buttermilk, is a low cost by-product from butter manufacture and available in large quantities but has been considered invaluable for many years. When cream is churned to butter the stable oil in water emulsion is destabilized and milk fat globule membrane (MFGM) is disrupted which is released in buttermilk. Thus, over the last two decades it has gained considerable attention due to its specific composition (proteins and polar lipids) attributed towards the MFGM. In account to this composition of buttermilk powder, it finds application in food industry as a natural stabilizing and emulsifying agent. It also finds application as a source of total solids and is commercially being added upto 10% in many food products. However, it has limitation in such applications due to the presence of cholesterol (approx. 80mg/100g). Cholesterol is also an integral part of MFGM and is released into buttermilk with MFGM. The most suitable techniques to remove cholesterol from buttermilk powder are using β-cyclodextrin and cholesterol oxidase enzyme. To valorize this abundantly produced industrial by-product of butter industry and increase its application in health food industry, as well as extend its application in pharmaceutical industry and preparation of liposomes, it is important to remove cholesterol from buttermilk. [less ▲]

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See detailNitrous oxide flux measurement with a closed chamber system : data treatment
Regaert, Donat ULg; Moureaux, Christine ULg; Heinesch, Bernard ULg et al

Poster (2015, January 30)

Nitrous oxide flux estimation from concentration measurements with a closed chamber system. Statistical data treatment to sort between relevant/irrelevant fluxes.

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See detailInfluence of cover crop management on sugar beet production
Hiel, Marie-Pierre ULg; Bodson, Bernard ULg

Poster (2015, January 30)

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See detailActivation of the calcium-sensing receptor before renal ischemia/reperfusion exacerbates kidney injury
WEEKERS, Laurent ULg; De Tullio, Pascal ULg; BOVY, Christophe ULg et al

in American Journal of Translational Research (2015), 7(1), 128-138

Activation of the calcium-sensing receptor (CaSR) by ischemia/reperfusion (I/R) favours apoptosis in cardiomyocytes, hepatocytes and neurons. Its role in renal I/R is unknown. We investigated the impact ... [more ▼]

Activation of the calcium-sensing receptor (CaSR) by ischemia/reperfusion (I/R) favours apoptosis in cardiomyocytes, hepatocytes and neurons. Its role in renal I/R is unknown. We investigated the impact of pharmacological preactivation of the CaSR on kidney structure and function in a murine model of bilateral renal 30-min ischemia and 48-hour reperfusion, and in a 6-year cohort of kidney transplant recipients (KTR). C57BL/6J mice were administered daily with CaSR agonist, R-568, or with vehicle for 48 hours. Evaluation of serum urea and creatinine levels, renal histology and urine metabolome by nuclear magnetic resonance showed that R-568 was not nephrotoxic per se. Following I/R, serum urea and creatinine levels increased higher in R-568-treated animals than in controls. Jablonski’s score was significantly greater in R-568-treated kidneys, which showed a higher rate of cell proliferation and apoptosis in comparison to controls. Next, we retrospectively identified 36 patients (10.7% of our cohort) who were treated by CaSR agonist, cinacalcet, at the time of kidney transplantation (KTx). After matching these to 61 KTR upon type of donor, cold ischemic time, residual diuresis, and donor age, we observed that delayed graft function, i.e. need for dialysis in the first week after KTx, occurred in 42 and 23% of cinacalcet-treated and control groups, respectively (p≤0.05). These data suggest that pharmacological preactivation of the CaSR before renal I/R exacerbates kidney injury. [less ▲]

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See detailInvestigating the Effects of Plant Root Exudates on PAHs Bioavailability to Soil Microorganisms in Contaminated Brownfields : Research Methodology.
Davin, Marie ULg; Lognay, Georges ULg; Fauconnier, Marie-Laure ULg et al

Poster (2015, January 30)

As a result of heavy industrial past activities, an estimated 6,000 brownfields require remediation in Wallonia. This number rises to over 3.5 million in Europe. Polycyclic Aromatic Hydrocarbons (PAHs ... [more ▼]

As a result of heavy industrial past activities, an estimated 6,000 brownfields require remediation in Wallonia. This number rises to over 3.5 million in Europe. Polycyclic Aromatic Hydrocarbons (PAHs) represent 17% of treated pollutants in Wallonia (Aldric et al., 2011). Current remediation techniques are rather expensive and technically demanding (Megharaj et al., 2011). Based on the observation that PAHs soil content decreases in the presence of plants (Cheema et al., 2010), the PhD aims at developing alternative PAHs remediation techniques in brownfields. It is articulated around three research axes. The first axis focusses on plant exudates and how they may improve PAHs bioavailability to soil microorganisms and enhance their degradation. This will be investigated by (i) characterizing several contaminated soils (physico-chemical parameters) and PAH content and factors of bioavailability, (ii) selecting a plant model and collecting root exudates, and (iii) evaluating the effects of exudates on PAHs bioavailability. The objective of the second axis is to evaluate the effects of plant exudates on PAHs degrading microorganisms by (i) comparing PAHs biodegradation in the presence/absence of exudates and (ii) assessing the potential toxic effects of exudate compounds on the microbial communities. The aim of the third axis is to study plant-pollutants interactions by (i) establishing the plant tolerance to several contamination levels and (ii) following PAHs bioavailability when facing real exudation rates, on the field. [less ▲]

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See detailLa participation : pour quoi faire ?
Mormont, Marc ULg

Conference given outside the academic context (2015)

La participation du public aux politiques environnementales peut prendre de multiples formes. Mais elle relève aussi de justifications différentes qui correspondent à des formes d'engagement très ... [more ▼]

La participation du public aux politiques environnementales peut prendre de multiples formes. Mais elle relève aussi de justifications différentes qui correspondent à des formes d'engagement très différentes. La contribution de la participation est légitime au plan légal et politique, mais son instrumentation est faible. L'auteur insiste sur deux critères cruciaux d'évaluation ; la délibération et les niveaux d'engagement des publics. [less ▲]

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See detailAccompagnement individualisé et pratique d’évaluation dans le cadre du master complémentaire Formasup
Jérôme, Françoise ULg; Delfosse, Catherine ULg; Detroz, Pascal ULg et al

in Detroz, Pascal; Borsu, Olivier (Eds.) Actes du 27ème colloque de l ADMEE Europe : l'évaluation à la lumière des contextes et des disciplines (2015, January 30)

Cet article rend compte de la réflexion d’une équipe de formateurs à propos du mode d’évaluation à appliquer aux travaux d’enseignants en formation disposant au départ d’un bagage de compétences ... [more ▼]

Cet article rend compte de la réflexion d’une équipe de formateurs à propos du mode d’évaluation à appliquer aux travaux d’enseignants en formation disposant au départ d’un bagage de compétences hétérogènes. Cette variété des compétences de départ a pour effet que les enseignants recourent de façon plus ou moins intensive à l’accompagnement pédagogique qui leur est proposé pour réaliser certaines tâches. Dans les cas d’un recours intensif à l’accompagnement se pose la question de la part de la note finale qui revient véritablement à l’enseignant-participant. Ces tensions entre équité et différentiation sont analysées dans le cadre du master complémentaire en pédagogie de l’enseignement supérieur organisé à l’Université de Liège (IFRES-FAPSE). Un outil est proposé pour les aborder : une grille visant à objectiver l’intensité de l’accompagnement dispensé/reçu et donc à apporter un éclairage plus nuancé sur les résultats de l’évaluation finale standardisée. [less ▲]

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See detailNumerical modeling of the long term behavior of Municipal Solid Waste in a landfill
Hubert, Julien ULg; Collin, Frédéric ULg

Scientific conference (2015, January 30)

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See detailAzacytidine prevents experimental sclerodermic chronic graft-versus-host disease
Fransolet, Gilles ULg; Ehx, Grégory ULg; SOMJA, Joan ULg et al

Poster (2015, January 30)

Introduction: Graft-versus-host disease (GVHD) remains one major complication of allogeneic hematopoietic stem cell transplantation (HSCT). Following unmanipulated peripheral-blood stem cell ... [more ▼]

Introduction: Graft-versus-host disease (GVHD) remains one major complication of allogeneic hematopoietic stem cell transplantation (HSCT). Following unmanipulated peripheral-blood stem cell transplantation, 60% of the patients experience chronic GVHD while approximately 15% of them develop a sclerodermic form of chronic GVHD characterized by multiple organ fibrosis and loss of skin elasticity. Regulatory T cells (Tregs) play a pivotal role in the pathology of chronic GVHD by inhibiting alloreactive conventional T cells. Several studies have shown the hypomethylating agent Azacytidine (Aza) can demethylate the master transcription factor of Treg (Forkhead box protein 3 factor, FoxP3), thus promoting Treg differentiation of conventional T cells. This work investigates the impact of Aza in a classical murine model of sclerodermic chronic GVHD (B10.D2  BALB/cJ). Methods: Lethally irradiated BALB/cJ recipient mice were injected with 107 bone marrow cells + 7.107splenocytes from B10.D2 donor mice. Recipients were treated with subcutaneous injections of Aza at the dose of 0,5 or 2 mg/kg every two days from day 10 to 30 following transplantation. Mice GVHD was evaluated with five criteria (weight loss, activity, fibrosis, hair loss and mice posture; 0-1-2 points/criteria). Mice were sacrificed at a score of 8/10 (or > 20% weight loss). Results: Mice treated with Aza 0.5 mg/kg (n = 14) or 2 mg/kg (n = 17) had significant lower clinical scores compared to control ones (n = 15) after treatment. FACS analysis showed a higher proportion of Treg among CD4+ T cells in the blood of Aza 2 mg/kg mice than in control mice (P = 0.047), as well as a higher percentage of Tregs expressing the KI67 proliferative marker on the same day (P = 0.0005). Finally, analyses of the cellular blood components with Cell-dyn demonstrated that Aza 2 mg/kg treated mice were significantly lymphopenic as compared to control mice (P = 0.05). Conclusion : Aza prevented sclerodermic GVHD in this classical murine model of chronic GVHD. [less ▲]

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See detailElicitation of the defence mechanisms at plant case of Cucumis melo
Ydjedd, Siham; Kati, D.E.; Fauconnier, Marie-Laure ULg

Poster (2015, January 30)

The judicious use of elicitors could reduce the amount of pesticide necessary to protect crops and preservation of the environment. This strategy frequently called "stimulation of natural defenses ... [more ▼]

The judicious use of elicitors could reduce the amount of pesticide necessary to protect crops and preservation of the environment. This strategy frequently called "stimulation of natural defenses" arouses more and more interest in the phytosanitary field. [less ▲]

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See detailInteractions of natural rhamnolpids produced by Pseudomonas aeruginosa with plant model membranes
Polo Lozano, Damien ULg; Nasir, Mehmet Nail ULg; Deleu, Magali ULg et al

Poster (2015, January 30)

It is well known that chemical pesticides have harmful effects on human health and environment. In this context, the interest for alternative products such as biopesticides is increasing. Among them ... [more ▼]

It is well known that chemical pesticides have harmful effects on human health and environment. In this context, the interest for alternative products such as biopesticides is increasing. Among them, elicitors act on the plants by inducing systemic resistance against diseases caused by fungal, viral, bacterial agents and insects. Rhamnolipids are surface active molecules produced mainly by various strains of the bacterium Pseudomonas aeruginosa. These secondary metabolites are composed of one to three fatty acids with various chain lengths linked through a glycosidic bond to one or two rhamnose moieties. The fatty acids are linked together through an ester bond. These molecules have shown several biological activities including plant defense stimulation. It has been suggested that this elicitor activity could be related to an interaction of rhamnolipids with the lipid bilayer of the plant plasma membrane (PPM) and lead to its destabilization, which can activate the plant defense signaling pathways. In this context, interactions of two rhamnolipids (Rha-C10-C10 and Rha-Rha-C10-C10) with biomimetic membranes of PPM such as Langmuir monolayers and multilayers were investigated using biophysical and in silico approaches. [less ▲]

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See detailFéminiser la voix
Morsomme, Dominique ULg; Remacle, Angélique ULg

Conference (2015, January 29)

Parlée ou chantée, notre voix révèle qui nous sommes. Elle participe à notre identité et véhicule nos émotions (ref. Revis. J). Dans cet exposé, nous présenterons la voix du point de vue du genre. En ... [more ▼]

Parlée ou chantée, notre voix révèle qui nous sommes. Elle participe à notre identité et véhicule nos émotions (ref. Revis. J). Dans cet exposé, nous présenterons la voix du point de vue du genre. En effet, l’Unité Logopédie de la Voix (www.logopedie-voix.be) s’intéresse entre autres à la féminisation vocale chez les personnes transidentitaires, en processus de changement de genre. Ces personnes ne s’identifiant pas au sexe et au genre qui leur ont été attribués à la naissance, elles éprouvent un désir profond de vivre comme une personne du sexe opposé à leur sexe biologique. Lors du processus de transition où ont lieu les transformations nécessaires au changement de genre, la logopédie a pour but d’améliorer la cohérence entre l’apparence de la personne et sa voix. Ainsi, notre recherche se focalise sur ce qui distingue la voix féminine de la voix masculine. Nous visons à développer des stratégies de féminisation vocale, portant sur des paramètres tels que la fréquence fondamentale de la voix (grave ou aigüe), les contours intonatifs, le niveau d’amplitude de certains formants, le débit de parole, son rythme, ou encore le vocabulaire choisi. Féminiser une voix, c’est chercher à comprendre ce qui permet d’en identifier le genre, mais également participer à la construction de l’identité de la personne à part entière. [less ▲]

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