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See detailIncreased electron donor and electron acceptor characters enhance the adhesion between oil droplets and cells of Yarrowia lipolytica as evaluated by a new cytometric assay
Aguedo, Mario ULg; Waché, Y.; Mazoyer, V. et al

in Journal of Agricultural and Food Chemistry (2003), 51(10), 3007-3011

The adhesion of methyl ricinoleate droplets to cells of the yeast Yarrowia lipolytica was investigated. A new cytometric method, relying on the double staining of fatty globules with Nile Red and of cells ... [more ▼]

The adhesion of methyl ricinoleate droplets to cells of the yeast Yarrowia lipolytica was investigated. A new cytometric method, relying on the double staining of fatty globules with Nile Red and of cells with Calcofluor, enabled us to quantify methyl ricinoleate droplet adhesion to cells precultured on a hydrophilic or on a hydrophobic carbon source. In this last case, droplet adsorption was enhanced and a MATS (microbial adhesion to solvents) test revealed that this increase was due to Lewis acid-base interactions and not to an increase in the hydrophobic properties of the cell surface. These preliminary results demonstrate that the developed cytometric method is promising for various applications concerning the study of interactions between microorganisms and an emulsified hydrophobic substrates. [less ▲]

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See detailMechanisms underlying the toxicity of lactone aroma compounds towards the producing yeast cells
Aguedo, Mario ULg; Beney, L.; Waché, Y. et al

in Journal of Applied Microbiology (2003), 94(2), 258-265

Aims: To study the fundamental mechanisms of toxicity of the fruity aroma compound γ-decalactone, that lead to alterations in cell viability during its biotechnological production by yeast cells; Yarrowia ... [more ▼]

Aims: To study the fundamental mechanisms of toxicity of the fruity aroma compound γ-decalactone, that lead to alterations in cell viability during its biotechnological production by yeast cells; Yarrowia lipolytica that is able to produce high amounts of this metabolite was used here as a model. Methods and Results: Lactone concentrations above 150 mg l-1 inhibited cell growth, depolarized the living cells and increased membrane fluidity. Infrared spectroscopic measurements revealed that the introduction of the lactone into model phospholipid bilayers, decreased the phase transition temperature. Moreover, the H+-ATPase activity in membrane preparations was strongly affected by the presence of the lactone. On the other hand, only a slight decrease in the intracellular pH occurred. Conclusions: We propose that the toxic effects of γ-decalactone on yeast may be initially linked to a strong interaction of the compound with cell membrane lipids and components. Significance and Impact of the Study: These findings may enable the elaboration of strategies to improve yeast cell viability during the process of lactones bioproduction. [less ▲]

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See detailEffet des bordures de champs sur les populations de Chrysopes en cultures maraîchères
Mignon, Jacques ULg; Colignon, Pierre; Haubruge, Eric ULg et al

in Phytoprotection (2003), 84(2), 121-128

Both diversity and density of lacewings depend on prey availability but also on plant composition near crops. The impact of field borders vegetation on abundance and diversity of Syrphidae and ... [more ▼]

Both diversity and density of lacewings depend on prey availability but also on plant composition near crops. The impact of field borders vegetation on abundance and diversity of Syrphidae and Coccinellidae is well known but very few studies have been realised to assess its effect on the abundance and diversity of Chrysopidae. In 2000 and 2001, the impact of adjacent habitats on lacewing populations was investigated in both carrot and broad bean open fields. Three types of field borders were selected: (1) set-asides mainly composed by Lolium perenne and Trifolium spp., (2) crops (wheat, beet or other vegetables) and (3) woody areas (mixture of Acer sp., Populus sp. and Salix sp.). Insects were caught using yellow pan traps. Chrysopidae populations were shown to be significantly lower in carrot and broad bean fields bordered with woodland. Lacewing and other Neuroptera species richness were always low and were discussed in relation with their role of pest predators associated with other aphidophagous insects. [less ▲]

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See detailCatabolism of hydroxyacids and biotechnological production of lactones by Yarrowia lipolytica
Waché, Y.; Aguedo, Mario ULg; Nicaud, J.-M. et al

in Applied Microbiology and Biotechnology (2003), 61(5-6), 393-404

The γ- and δ-lactones of less than 12 carbons constitute a group of compounds of great interest to the flavour industry. It is possible to produce some of these lactones through biotechnology. For ... [more ▼]

The γ- and δ-lactones of less than 12 carbons constitute a group of compounds of great interest to the flavour industry. It is possible to produce some of these lactones through biotechnology. For instance, γ-decalactone can be obtained by biotransformation of methyl ricinoleate. Among the organisms used for this bioproduction, Yarrowia lipolytica is a yeast of choice. It is well adapted to growth on hydrophobic substrates, thanks to its efficient and numerous lipases, cytochrome P450, acylCoA oxidases and its ability to produce biosurfactants. Furthermore, genetic tools have been developed for its study. This review deals with the production of lactones by Y. lipolytica with special emphasis on the biotransformarion of methyl ricinoleate to γ-decalactone. When appropriate, information from the lipid metabolism of other yeast species is presented. [less ▲]

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See detailTranscription-coupled TA and GC strand asymmetries in the human genome
Touchon, M.; Nicolay, Samuel ULg; Arneodo, Alain et al

in FEBS Letters (2003), 555

Abstract Analysis of the whole set of human genes reveals that most of them present TA and GC skews, that these biases are correlated to each other and are speci¢c to gene sequences, exhibiting sharp ... [more ▼]

Abstract Analysis of the whole set of human genes reveals that most of them present TA and GC skews, that these biases are correlated to each other and are speci¢c to gene sequences, exhibiting sharp transitions between transcribed and non-tran- scribed regions. The GC asymmetries cannot be explained solely by a model previously proposed for (G+T) skew based on tran- sitions measured in a small set of human genes. We propose that the GC skew results from additional transcription-coupled mu- tation process that would include transversions. During evolu- tion, both processes acting on a large majority of genes in germ- line cells would have produced these transcription-coupled strand asymmetries. [less ▲]

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See detailProgesterone, cortisol and pregnancy-associated glycoprotein concentrations in three aborted cows
Alomar, M.; Sulon, J.; El Amiri, B. et al

in Proceedings of the 8e Journée de rencontre Bioforum (2003)

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See detailAdult Growth Hormone Deficiency
Beckers, Albert ULg; Beckers, Albert ULg

Textual, factual or bibliographical database (2003)

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See detailPituitary Adenomas
Beckers, Albert ULg; Beckers, Albert ULg

Textual, factual or bibliographical database (2003)

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See detailThe Genetics of Growth Hormone Axis
Beckers, Albert ULg; Beckers, Albert ULg

Textual, factual or bibliographical database (2003)

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See detailDioxins, furans and dioxin-like PCBs in juvenile harbour porpoises (Phocoena Phocoena) from the North sea
Beans, Cristina; Debacker, Virginie ULg; Jauniaux, Thierry ULg et al

in Organohalogen Compounds (2003)

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See detailWalking for pleasure in Belgium
Hanocq, Philippe ULg

in Busi, Roberto; Pezzagno, Michèle (Eds.) Living and walking in cities 8th International conference (2003)

Description of an original and ambitious project of the regional Walloon government (Belgium) intending for the promotion of not motorised travelling modes : the “RAVeL project”.

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See detailAnalysis of PCDD/Fs in human blood plasma using CALUX bioassay and GC-HRMS: a comparison
Van Wouwe, Nathalie; Eppe, Gauthier ULg; Xhrouet, Céline ULg et al

in Organohalogen Compounds (2003)

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See detailSelective adsorption of dioxins and PCBs from marine oils on activated carbon
De Meulenaer, Bruno; Maes, Jeroen; Van Heerswynghels, Peter et al

in Organohalogen Compounds (2003)

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See detailValidation and discussion of CALUX analysis for marine samples
Windal, Isabelle; Van Wouwe, Nathalie; Carbonnelle, Sophie et al

in Organohalogen Compounds (2003)

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See detailLe cas clinique du mois. La maladie de still de l'adulte: une cause rare d'hepatite aigue febrile
Michels, V.; Delwaide, Jean ULg; Vermeulen, P. et al

in Revue Médicale de Liège (2003), 58(12), 729-733

A 63-year-old woman was hospitalized for the third time in one year for asthenia, fever and chills, jaundice, cytolysis and cholestasis. An adult onset Still's disease was diagnosed. Hepatic ... [more ▼]

A 63-year-old woman was hospitalized for the third time in one year for asthenia, fever and chills, jaundice, cytolysis and cholestasis. An adult onset Still's disease was diagnosed. Hepatic manifestations, diagnostic criteria and efficient therapy of AOSD will be reviewed. [less ▲]

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See detailLe rêve pavillonnaire et la logique du développement durable
Halleux, Jean-Marie ULg

in Le lotissement comme outil qualitatif du développement des villages. Actes du colloque du 4 novembre 2003 (2003)

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See detailA propos de la prescription des mensualités d'un crédit
Biquet, Christine ULg

in Annuaire juridique du crédit et du règlement collectif de dettes (2003)

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