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See detailFalse phylogenies on wood mice due to cryptic cytochrome-b pseudogene.
Dubey, Sylvain; Michaux, Johan ULg; Brunner, Harald et al

in Molecular Phylogenetics & Evolution (2009), 50(3), 633-41

The phylogeny and phylogeography of the Old World wood mice (subgenus Sylvaemus, genus Apodemus, Muridae) are well-documented. Nevertheless, the distributions of species, such as A. fulvipectus and A ... [more ▼]

The phylogeny and phylogeography of the Old World wood mice (subgenus Sylvaemus, genus Apodemus, Muridae) are well-documented. Nevertheless, the distributions of species, such as A. fulvipectus and A. ponticus remain dubious, as well as their phylogenetic relationships with A. sylvaticus. We analysed samples of Apodemus spp. across Europe using the mitochondrial cytochrome-b gene (cyt-b) and compared the DNA and amino-acid compositions of previously published sequences. The main result stemming from this study is the presence of a well-differentiated lineage of Sylvaemus including samples of various species (A. sylvaticus, A. fulvipectus, A. ponticus) from distant locations, which were revealed to be nuclear copies of the mitochondrial cyt-b. The presence of this cryptic pseudogene in published sequences is supported by different pathways. This has led to important errors in previous molecular trees and hence to partial misinterpretations in the phylogeny of Apodemus. [less ▲]

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See detailSpecies identification, molecular sexing and genotyping using non-invasive approaches in two wild bovids species: Bos gaurus and Bos javanicus.
Riviere-Dobigny, Taiana; Doan, Lan Pham; Quang, Nam Le et al

in Zoo Biology (2009), 28(2), 127-36

Since the second Indochina war, habitat destruction and overhunting has resulted in fragmentation of the remaining populations of Bos javanicus and B. gaurus. Nowadays, both species are in serious danger ... [more ▼]

Since the second Indochina war, habitat destruction and overhunting has resulted in fragmentation of the remaining populations of Bos javanicus and B. gaurus. Nowadays, both species are in serious danger, especially the gaur. In Vietnam, where these species have become almost impossible to capture in the wild, non-invasive investigations are the only feasible approach to obtain data on populations. However, non-invasive derived DNA, especially in tropical areas, is usually characterized by low concentrations, poor quality and/or contamination from alien DNA. To assist in tropical conservation management, baseline information is provided here on assessing the reliability of species identification, molecular sexing and microsatellite genotyping using fecal DNA from B. gaurus and B. javanicus. For species identification using bovine fecal samples, cytochrome b fragment between positions 867 and 1140 was found to contain species diagnostic sites, which distinguishes the four species encountered in the region: B. gaurus, B. indicus, B. javanicus and B. taurus. For sex determination, primers were initially tested on DNA obtained from blood. Then, these primers were successfully used on DNA derived from fecal material. Finally, we also evaluate the feasibility of non-invasive microsatellite genotyping on fecal samples collected in Vietnamese nature reserves. The results presented here improve on current molecular methods based on fecal material obtained from tropical areas. [less ▲]

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See detailConcentrations of chlorinated and brominated contaminants and their metabolites in serum of harbour seals and harbour porpoises
Weijs, Liesbeth; Das, Krishna ULg; Siebert, Ursula et al

in Environment International (2009), 35(6), 842-850

Harbour seals (Phoca vitulina) and harbour porpoises (Phocoena phocoena) are top predators in the North Sea and consequently accumulate a variety of pollutants in their tissues. Concentrations of ... [more ▼]

Harbour seals (Phoca vitulina) and harbour porpoises (Phocoena phocoena) are top predators in the North Sea and consequently accumulate a variety of pollutants in their tissues. Concentrations of polychlorinated biphenyls (PCBs), polybrominated diphenyl ethers (PBDEs) and their hydroxylated metabolites (HO-PCBs and HO-PBDEs) were measured in serum of wild harbour seals (n=47) and captive harbour porpoises (n=21). Both species exhibit long life spans and do not have extreme situations, such as complete fasting during periods of lactation, in their annual cycles. For PCBs, concentrations in adult males were slightly higher than in juveniles and lowest in juvenile females. For PBDEs, juveniles have higher levels than adult males and females, probably as a consequence of lactational transfer. However. differences between these age-gender groups were not statistical significant, indicating that individual variation was limited within each species, even without knowing the feeding status of the animals. Body condition. particularly emaciation, has a major influence on the levels of chlorinated and brominated contaminants in serum. Profiles of PCBs were CB 153>CB 138>CB 187>CB 180 and CB 153>CB 138>CB 149>CB 187>CB 180 for harbour seals and porpoises respectively. For PBDEs, BDE 47 was the predominant congener followed by BDE 100 and 99 in both species. In harbour seals, concentrations of sum PCBs (median: 39,200 pg/ml) were more than 200 times higher than levels of sum PBDEs (median: 130 pg/ml) and almost 10 times higher than concentrations of sum HO-PCBs (4350 pg/ml). In harbour porpoises, concentrations of sum PCBs (median: 24,300 pg/ml) were about 20 times higher than concentrations of PBDEs (median: 1300 pg/ml). HO-PCBs were detected in only 4 harbour porpoises and this at very low concentrations. Naturally-produced MeO-PBDEs were only found in harbour porpoises at concentrations ranging from 120 to 810 pg/ml. HO-PBDEs were not found in any species. In general, harbour seals accumulate less compounds and have mostly lower concentrations than harbour porpoises possibly as a result of a better developed metabolism. (C) 2009 Elsevier Ltd. All rights reserved. [less ▲]

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See detailDecalcifying odontocete ears following a routine protocol with RDO (R)
Morell, M.; Degollada, E.; Alonso, J. M. et al

in Journal of Experimental Marine Biology & Ecology (2009), 376(2), 55-58

The study of the organ of Corti is essential to assess the impact of underwater noise on cetaceans. While classical histology techniques (including EDTA decalcification) have been previously considered ... [more ▼]

The study of the organ of Corti is essential to assess the impact of underwater noise on cetaceans. While classical histology techniques (including EDTA decalcification) have been previously considered, the process is time consuming. Independently from the histological technique, one of the challenging steps after extraction and fixation of the samples is to decalcify the bone envelope to access the cochlea without damaging the soft tissues. Here, we propose to use a fast commercial decalcifier (RDO (R)). 93 ears from 11 different odontocetes species stranded in the Mediterranean, Spanish North Atlantic and North Sea were used to precisely determine the decalcification time. Depending on the tympanic-periotic volume of the species, the decalcification time ranged from several hours to a few days, allowing a subsequently faster observation of the cochlear structures through routine microscope techniques. (C) 2009 Elsevier B.V. All rights reserved. [less ▲]

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See detailEvaluations of cognitive, linguistic and mnemonic competencies of people with Down’s syndrome; An integrative conception of mental retardation
Frenkel, Stéphanie ULg

in Journal of Cognitive Education and Psychology (2009), 8(2), 214-215

Thesis summary

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See detailInsight into the role of catalases in salt stress in potato (Solanum tuberosum L.).
M'Hamdi, M.; Bettaieb, T.; Harbaoui, Y. et al

in Biotechnologie, Agronomie, Société et Environnement = Biotechnology, Agronomy, Society and Environment [=BASE] (2009), 13

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See detailVisite guidée au sein du noyau cellulaire
Thiry, Marc ULg

Scientific conference (2009)

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See detailFoy Notre-Dame : Art, Politique et Religion. Actes du colloque de Dinant (16 mai 2009)
Delfosse, Annick ULg; Dekoninck, Ralph, ; Pacco, Christian,

Book published by Société archéologique de Namur (2009)

Ouvrage collectif consacré à l'étude de la statue miraculeuse de Notre-Dame de Foy, de son sanctuaire et de son culte sous deux angles : artistique et historique. La première partie traite du sanctuaire ... [more ▼]

Ouvrage collectif consacré à l'étude de la statue miraculeuse de Notre-Dame de Foy, de son sanctuaire et de son culte sous deux angles : artistique et historique. La première partie traite du sanctuaire et des supports de dévotion. La deuxième partie dégage la signification du culte marial à Foy dans le contexte politique et religieux du XVIIe siècle et au-delà. [less ▲]

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See detailProSup, référentiel des compétences des enseignants du supérieur
Poumay, Marianne ULg; Georges, François ULg

Cartographic material (2009)

La carte représente les compétences poursuivies dans le master complémentaire en pédagogie universitaire pour servir le développement de l'expertise d'encadrant dans le domaine de l'enseignement.

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See detailUrban ontologies for an improved communication in urban development projects
Teller, Jacques ULg; Cutting-Decelle, Anne-Françoise; Billen, Roland ULg

Book published by Les éditions de l'Université de Liège (2009)

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See detailQualitative spatial reasoning
Billen, Roland ULg; Van de Weghe, Nico

in Thrift, Nigel; Kitchin, Rob (Eds.) International Encyclopaedia of Human Geography (2009)

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See detailCollaboration industrielle et enseignement par projet en électronique de puissance
Plumier, Frédéric ULg; Bleus, Paul; Geuzaine, Christophe ULg

in J3eA : Journal sur l'Enseignement des Sciences et Technologies de l'Information et des Systèmes (2009), 8(Hors série 1), 1015

For the first time during year 2007-2008, a complete design of a DC/DC converter, based on specifications only, was asked to the students following the basic Power Electronics course at University of ... [more ▼]

For the first time during year 2007-2008, a complete design of a DC/DC converter, based on specifications only, was asked to the students following the basic Power Electronics course at University of Liège. This very realistic pedagogical way of teaching helped the students to be autonomous, to learn project and team work. [less ▲]

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See detailLa mannheimiose : d’une liaison (moléculaire) fatale à une des principales maladies d’élevage des ruminants
Fett, Thomas ULg; Zecchinon, Laurent; Vanden Bergh, Philippe et al

in Annales de Médecine Vétérinaire (2009), 153(1), 31-53

Les pneumonies bactériennes constituent un problème majeur dans l'élevage et l'engraissement des bovins, avec des répercussions très élevées en termes de morbidité et de mortalité. Parmi les différentes ... [more ▼]

Les pneumonies bactériennes constituent un problème majeur dans l'élevage et l'engraissement des bovins, avec des répercussions très élevées en termes de morbidité et de mortalité. Parmi les différentes causes biologiques recensées, Mannheimia haemolytica tient le haut de l’affiche puisqu’elle est connue comme agent pathogène compliquant principal, voire systématique. Une de ses particularités est qu’elle ne déclenche de pneumonie broncho-alvéolaire fibrino-hémorragique que chez les ruminants. Les lésions observées résultent d’une nécrose en masse des neutrophiles et macrophages et du relarguage de leurs différents constituants. Au niveau moléculaire, cette spécificité trouve son origine dans l’intimité de la liaison entre la leucotoxine, le facteur de virulence majeur de la bactérie, et la sous-unité CD18 des récepteurs de surface leucocytaires bêta2-intégrines. La revue décrit cette liaison et ses conséquences aux niveaux macroscopique et microscopique, avant de faire le point sur les dernières avancées de la recherche et d’ébaucher quelques perspectives thérapeutiques. [less ▲]

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See detailTwo-year clinical, angiographic, and intravascular ultrasound follow-up of the XIENCE V everolimus-eluting stent in the treatment of patients with de novo native coronary artery lesions: the SPIRIT II trial.
Claessen, Bimmer E.; Beijk, Marcel A.; Legrand, Victor ULg et al

in Circulation. Cardiovascular interventions (2009), 2(4), 339-47

BACKGROUND: This article reports the 2-year clinical, angiographic, and intravascular ultrasound outcomes of the everolimus-eluting stent (EES) compared with the paclitaxel-eluting stent (PES) in the ... [more ▼]

BACKGROUND: This article reports the 2-year clinical, angiographic, and intravascular ultrasound outcomes of the everolimus-eluting stent (EES) compared with the paclitaxel-eluting stent (PES) in the randomized SPIRIT II trial. METHODS AND RESULTS: This was a prospective, single-blind clinical trial in which a total of 300 patients with de novo native coronary artery lesions were randomized to either EES or PES in a 3:1 fashion. Clinical follow-up was planned at 2 years in all patients. A subset of 152 patients underwent serial angiographic and intravascular ultrasound analyses at 6 months and 2 years. After 2 years, target lesion failure (cardiac death, myocardial infarction, and ischemia-driven target lesion revascularization) rates were 6.6% and 11% in EES and PES, respectively (P=0.31). At 6 months, a significant reduction in angiographic in-stent late loss and percentage volume obstruction measured by intravascular ultrasound was observed in the EES group. However, at 2-year follow-up, a late increased intimal hyperplasia growth after implantation of an EES was observed. There were no significant differences between EES and PES for in-stent late loss (EES, 0.33+/-0.37 mm versus PES, 0.34+/-0.34 mm; P=0.84) and percentage volume obstruction (EES, 5.18+/-6.22% versus PES, 5.80+/-6.31%; P=0.65) at 2 years. The incidence of stent thrombosis was low and comparable in both groups (EES, 0.9%; PES, 1.4%). CONCLUSIONS: Although the previously reported angiographic and clinical superiority of the EES has vanished over time, this report confirms and extends the previously demonstrated noninferiority in terms of in-stent late loss of the EES when compared with the PES up to 2-year follow-up. There were no significant differences between EES and PES in clinical, angiographic and intravascular ultrasound outcomes at 2 years. [less ▲]

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