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See detailPrevalence of enterohaemorrhagic Escherichia coli from serotype O157 and other attaching and effacing Escherichia coli on bovine carcasses in Algeria
Chahed, Amina; China, Bernard; Mainil, Jacques ULg et al

in Journal of Applied Microbiology (2006), 101(2), 361-368

AIMS: Bovine meat is the principal source of human contamination of attaching and effacing Escherichia coli, including enterohaemorrhagic E. coli O157. The aim was to study the prevalence of these strains ... [more ▼]

AIMS: Bovine meat is the principal source of human contamination of attaching and effacing Escherichia coli, including enterohaemorrhagic E. coli O157. The aim was to study the prevalence of these strains on bovine carcasses in Algeria. METHODS AND RESULTS: Two-hundred and thirty carcasses were swabbed and analysed by classical microbiological methods for total E. coli counts and for the presence of pathogenic E. coli. The E. coli counts were high, with a 75th percentile of 444.75 CFUs cm(-2). For pathogenic E. coli, more than 7% of the tested carcasses were positive for E. coli O157. Eighteen E. coli O157 strains were isolated and typed by multiplex PCR. The main isolated pathotype (78%) was eae+ stx2+ ehxA+. In addition to E. coli O157, other attaching and effacing E. coli (AEEC) were also detected from carcasses by colony hybridization after pre-enrichment and plating on sorbitol MacConkey agar using eae, stx1 and stx2 probes. Thirty carcasses (13%) on the 230 analysed harboured at least one colony positive for one of the tested probes. These positive carcasses were different from those positive for E. coli O157. Sixty-six colonies (2.9%) positive by colony hybridization were isolated. The majority (60.6%) of the positive strains harboured an enteropathogenic E. coli-like pathotype (eae+ stx-). Only three enterohaemorrhagic E. coli (EHEC)-like (eae+ stx1+) colonies were isolated from the same carcass. These strains did not belong to classical EHEC serotypes. CONCLUSIONS: In this study, the global hygiene of the slaughterhouse was low, as indicated by the high level of E. coli count. The prevalence of both E. coli O157 and other AEEC was also high, representing a real hazard for consumers. SIGNIFICANCE AND IMPACT OF THE STUDY: This is the first study of this type in Algeria, which indicates that the general hygiene of the slaughterhouse must be improved. [less ▲]

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See detailPreparation of YBa2Cu3O7-x superconducting thick films by the electrophoretic deposition method
Dusoulier, Laurent; Denis, Samuel ULg; Vanderbemden, Philippe ULg et al

in Journal of Materials Science (2006), 41(24), 8109-8114

YBa2Cu3O7-x thick films have been realised by the Electrophoretic Deposition method (EPD). The influence of several parameters (powder and iodine concentrations in the suspension, applied voltage and ... [more ▼]

YBa2Cu3O7-x thick films have been realised by the Electrophoretic Deposition method (EPD). The influence of several parameters (powder and iodine concentrations in the suspension, applied voltage and deposition time) on the EPD process has been studied by measuring the conductivity of the suspension and the amount of YBa2Cu3O7-x particles deposited on the electrode. Superconducting coatings onto silver substrates have been produced by a multilayer process during different deposition times. The highest critical current density value of these coatings measured by the four-point probe method is about 10^3 A/cm^2 (77 K), in a suitable range for magnetic shielding applications. [less ▲]

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See detailProlonged viral RNA detection in blood and lymphoid tissues from Coxsackievirus B4 E2 orally-inoculated Swiss mice
Jaidane, H.; Gharbi, J.; Lobert, P. E. et al

in Microbiology and Immunology (2006), 50(12), 971-974

The spreading of viral RNA within Swiss Albino mice orally inoculated with coxsackievirus B4 E2 strain (CVB4 E2) was studied by using RT-PCR and semi-nested-RT-PCR methods. Viral RNA was detected in ... [more ▼]

The spreading of viral RNA within Swiss Albino mice orally inoculated with coxsackievirus B4 E2 strain (CVB4 E2) was studied by using RT-PCR and semi-nested-RT-PCR methods. Viral RNA was detected in various organs: pancreas, heart, small intestine, spleen, thymus, and blood at various post-infectious (p.i.) times ranging from 8 hr to 150 days. Our results show that (i) outbred mice can be infected with CVB4 E2 following an oral inoculation, which results in systemic spreading of viral RNA, (ii) CVB4 E2 infection can be associated with a prolonged detection of viral RNA in spleen, thymus and blood, up to 70 days p.i. and further in other organ tissues. [less ▲]

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See detailPylephlebitis of the portal vein complicating intragastric migration of an adjustable gastric band
De Roover, Arnaud ULg; Detry, Olivier ULg; Coimbra Marques, Carla ULg et al

in Obesity Surgery (2006), 16(3), 369-371

Pylephlebitis, or septic thrombophlebitis of the portal vein, is an infrequent but life-threatening complication of abdominal septic events. The authors report the occurrence of pylephlebitis and multiple ... [more ▼]

Pylephlebitis, or septic thrombophlebitis of the portal vein, is an infrequent but life-threatening complication of abdominal septic events. The authors report the occurrence of pylephlebitis and multiple liver abscesses induced by a neglected intra-gastric migration of an adjustable silicone gastric band. The patient was successfully treated by broad-spectrum antibiotics and total gastrectomy with Roux-en-Y esophagojejunostomy. Postoperative recovery was marked by acute liver failure that was managed conservatively. The patient is alive and well at 1-year follow-up. This case emphasizes the interest in early removal of the band when intra-gastric migration is diagnosed. [less ▲]

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See detailResidual and contaminants of alimentary commodity: 25 years of their analysis progress. 2. Biological methods of detection
Scippo, Marie-Louise ULg; Maghuin-Rogister, Guy ULg

in Annales de Médecine Vétérinaire (2006), 150(2), 125-130

This article presents a synthesis of the various biological methods used to detect residues or contaminants in foods, by insisting on their evolution during these last 25 years and by illustrating with ... [more ▼]

This article presents a synthesis of the various biological methods used to detect residues or contaminants in foods, by insisting on their evolution during these last 25 years and by illustrating with numerous examples. The biological methods are based on the detection of a biological effect of the analyte of interest. In the years 60-70, this effect was detected by means of histology. At present, the biological tests use cells in culture genetically modified to contain a reporter gene, activated when the analyte of interest is present in the culture medium. [less ▲]

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See detailRole of acetaldehyde in mediating the pharmacological and behavioral effects of alcohol
Quertemont, Etienne ULg; Didone, Vincent ULg

in Alcohol Research & Health : The Journal of the National Institute on Alcohol Abuse & Alcoholism (2006), 29(4), 258-265

Acetaldehyde is the first active breakdown product (i.e., metabolite) generated during alcohol metabolism. It has toxic properties but also exerts other actions on the body (i.e., has pharmacological ... [more ▼]

Acetaldehyde is the first active breakdown product (i.e., metabolite) generated during alcohol metabolism. It has toxic properties but also exerts other actions on the body (i.e., has pharmacological properties). Recent studies have shown that the direct administration of acetaldehyde, especially into the brain, induces several effects that mimic those of alcohol. High doses of acetaldehyde induce sedative as well as movement-and memory-impairing effects, whereas lower doses produce behavioral effects (e.g., stimulation and reinforcement) that are characteristic of addictive drugs. When acetaldehyde accumulates outside the brain (i.e., in the periphery), adverse effects predominate and prevent further alcohol drinking. To investigate the role of acetaldehyde in mediating alcohol's effects, investigators have pharmacologically manipulated alcohol metabolism and the production of acetaidehyde within the body (i.e., endogenous acetaldehyde production). Studies manipulating the activity of the enzyme catalase, which promotes acetaldehyde production in the brain, suggest that acetaldehyde contributes to many behavioral effects of alcohol, especially its stimulant properties. However, it remains controversial whether acetaldehyde concentrations obtained under normal physiological conditions are sufficient to induce significant pharmacological effects. Current evidence suggests that the contribution of acetaldehyde to alcohol's effects is best explained by a process in which acetaidehyde modulates, rather than mediates, some of alcohol's effects. [less ▲]

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See detailZn, Cu, Cd and Hg Binding to Metallothioneins in Harbour Porpoises Phocoena Phocoena from the Southern North Sea
Das, Krishna ULg; De Groof, Arnaud; Jauniaux, Thierry ULg et al

in BMC Ecology (2006), 6

BACKGROUND: Harbour porpoises Phocoena phocoena from the southern North Sea are known to display high levels of Zn and Hg in their tissues linked to their nutritional status (emaciation). The question ... [more ▼]

BACKGROUND: Harbour porpoises Phocoena phocoena from the southern North Sea are known to display high levels of Zn and Hg in their tissues linked to their nutritional status (emaciation). The question arises regarding a potential role of metallothioneins (MTs) with regard to these high metal levels. In the present study, metallothionein detection and associated Zn, Cd, Cu and Hg concentrations were investigated in the liver and kidney of 14 harbour porpoises collected along the Belgian coast. RESULTS: Metallothioneins seemed to play a key role in essential metal homeostasis, as they were shown to bind 50% of the total hepatic Zn and 36% of the total hepatic Cu concentrations. Renal MTs also participated in Cd detoxification, as they were shown to bind 56% of the total renal Cd. Hg was mainly found in the insoluble fraction of both liver and kidney. Concomitant increases in total Zn concentration and Zn bound to MTs were observed in the liver, whereas Zn concentration bound to high molecular weight proteins remained constant. Cu, Zn and Cd were accumulated preferentially in the MT fraction and their content in this fraction increased with the amount in the hepatocytosol. CONCLUSION: MTs have a key role in Zn and Cu homeostasis in harbour porpoises. We demonstrated that increasing hepatic Zn concentration led to an increase in Zn linked to MTs, suggesting that these small proteins take over the Zn overload linked to the poor body condition of debilitated harbour porpoises. [less ▲]

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See detailVariability in the impairments of recognition memory in patients with frontal lobe lesions
Bastin, Christine ULg; Van der Linden, Martial ULg; Lekeu, Françoise ULg et al

in Cortex : A Journal Devoted to the Study of the Nervous System & Behavior (2006), 42(7), 971-1058

Fourteen patients with frontal lobe lesions and 14 normal subjects were tested on a recognition memory task that required discriminating between target words, new words that are synonyms of the targets ... [more ▼]

Fourteen patients with frontal lobe lesions and 14 normal subjects were tested on a recognition memory task that required discriminating between target words, new words that are synonyms of the targets and unrelated distractors. A deficit was found in 12 of the patients. Moreover, three different patterns of recognition impairment were identified: (I) poor memory for targets, (II) normal hits but increased false recognitions for both types of distractors, (III) normal hit rates, but increased false recognitions for synonyms only. Differences in terms of location of the damage and behavioural characteristics between these subgroups were examined. An encoding deficit was proposed to explain the performance of patients in subgroup I. The behavioral patterns of the patients in subgroups II and III could be interpreted as deficient postretrieval verification processes and an inability to recollect item-specific information, respectively. [less ▲]

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See detailTexturation of YBa2Cu3O7-delta thick films by electrophoretic deposition under magnetic field
Dusoulier, Laurent; Denis, Samuel ULg; Nutal, Nicolas ULg et al

in Key Engineering Materials [=KEM] (2006), 314

YBa2Cu3O7-delta coatings were deposited by electrophoretic deposition (EPD) onto Ni substrates. Particles of different sizes and shapes were used in order to study the influence of the powder ... [more ▼]

YBa2Cu3O7-delta coatings were deposited by electrophoretic deposition (EPD) onto Ni substrates. Particles of different sizes and shapes were used in order to study the influence of the powder microstructure on the film density. Texturation of the thick films was induced by application of a magnetic field during the electrophoretic deposition. X-ray diffraction analysis has clearly shown preferred c-axis alignment of the YBa2Cu3O7-delta films along the direction normal to the substrate surface. Scanning electron microscopy and optical polarised light microscopy were used to characterise the microstructure of the coatings, revealing a nonrandom platelets organisation. [less ▲]

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See detailTabagisme occasionnel de l'adolescent, ou commence la nocivite?
Bartsch, Pierre ULg; Laperche, J.

in Revue Médicale de Liège (2006), 61(5-6, May-Jun), 394-400

It is clear that even low rate smoking is hazardous for health, the risk being independently increased by the daily number of cigarettes smoked and by the duration of smoking. The question raised is thus ... [more ▼]

It is clear that even low rate smoking is hazardous for health, the risk being independently increased by the daily number of cigarettes smoked and by the duration of smoking. The question raised is thus: will an adolescent experimenter be a non smoker, an experimenter for ever, a regular smoker, light or heavy? This short review shows that there are numerous factors from genetics, to familial environment not limited to tobacco issues, smoking situation at school as well as school performances of the student, and also individual psychological characteristics. The experimenter is a very good target for smoking cessation actions and should deserve particular attention from preventive medicine and, thus, from school medicine, before he becomes a regular smoker, who will be more resistant to smoking cessation programs. [less ▲]

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See detailThe concept of iso-inertial assessment: Reproducibility analysis and descriptive data
Jidovtseff, Boris ULg; Croisier, Jean-Louis ULg; Lhermerout, Claude ULg et al

in Isokinetics & Exercise Science (2006), 14(1), 53-62

The purpose of this study was to investigate the reproducibility of findings derived from a new iso-inertial dynamometer during bench press (BP) and squat (SQ) and to provide descriptive data for ... [more ▼]

The purpose of this study was to investigate the reproducibility of findings derived from a new iso-inertial dynamometer during bench press (BP) and squat (SQ) and to provide descriptive data for recreational athletes. A position transducer and accelerometer were combined to assess velocity and power during free weight lifting exercises. Simulated movement with a pulley system revealed the excellent technical consistency of the dynamometer. Sixteen male subjects participated in the study. Iso-inertial tests consisted of lifting as fast as possible four different relative loads (35, 50, 70, 90% 1RM in BP and 45, 60, 75, 90% 1RM in SQ). The test was repeated one week later. Analysis of variance revealed no significant variation between sessions or trials. Reproducibility was better in velocity than in power, although it remained fairly good in both exercises (coefficients of variation [CV] never exceeding 10%) except for the time to peak power parameter. Descriptive data confirmed the classical force-velocity and force-power relationships for BP and SQ. In conclusion, this study demonstrated reliable measurements in BP and SQ iso-inertial exercises. Monitoring force-velocity and force-power relationships offers an original functional approach in strength training supervision. [less ▲]

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See detailTeaching communication and stress management skills to junior physicians dealing with cancer patients: a Belgian Interuniversity Curriculum
Bragard, Isabelle ULg; Razavi, Darius; Marchal, Serge et al

in Supportive Care in Cancer (2006), 14(5), 454-461

Background: Ineffective physicians' communication skills have detrimental consequences for patients and their relatives, such as insufficient detection of psychological disturbances, dissatisfaction with ... [more ▼]

Background: Ineffective physicians' communication skills have detrimental consequences for patients and their relatives, such as insufficient detection of psychological disturbances, dissatisfaction with care, poor compliance, and increased risks of litigation for malpractice. These ineffective communication skills also contribute to everyday stress, lack of job satisfaction, and burnout among physicians. Literature shows that communication skills training programs may significantly improve physicians' key communication skills, contributing to improvements in patients' satisfaction with care and physicians' professional satisfaction. This paper describes a Belgian Interuniversity Curriculum (BIC) theoretical roots, principles, and techniques developed for junior physicians specializing in various disciplines dealing with cancer patients. Curriculum description: The 40-h training focuses on two domains: stress management skills and communication skills with cancer patients and their relatives. The teaching method is learner-centered and includes a cognitive, behavioral, and affective approach. The cognitive approach aims to improve physicians' knowledge and skills on the two domains cited. The behavioral approach offers learners the opportunity to practice these appropriate skills through practical exercises and role plays. The affective approach allows participants to express attitudes and feelings that communicating about difficult issues evoke. Such an intensive course seems to be necessary to facilitate the transfer of learned skills in clinical practice. Conclusions: The BIC is the first attempt to bring together a stress management training course and a communication training course that could lead not only to communication skills improvements but also to burnout prevention. [less ▲]

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See detailValidation analytique de l'ImmunoCap 250 (Sweden Diagnostics)
Cavalier, Etienne ULg; Carlisi, Agnès; Chapelle, Jean-Paul ULg

in Annales de Biologie Clinique (2006), 64(1, Jan-Feb), 91-4

A new automate, ImmunoCap 250 has recently been released by Sweden Diagnostics for the determination of specific and total IgE. We have evaluated its analytical performances (intra- and inter-assay ... [more ▼]

A new automate, ImmunoCap 250 has recently been released by Sweden Diagnostics for the determination of specific and total IgE. We have evaluated its analytical performances (intra- and inter-assay precision, linearity, recovery and sensibility) and compared the results obtained by Unicap 100 on 149 samples positive for 12 different allergens and 30 samples containing different concentrations of total IgE. Intra- and inter-assay precision are less than 10% excepted for the specific IgE of one allergen (i3, common wasp), but still in accordance with the NCCLS guidelines. The sensibility is 0.01 kU(A)/L for specific and 0.35 kU/L for total IgE. The method is linear up to 5000 kU/L for total and at least 80 kU(A)/L for specific IgE. The recovery test showed a recovery of the International standard WHO 75/502 between 85 and 115%, with a mean of 103%. The correlation coefficient between the ImmunoCap 250 and Unicap 100 is 0.9866 and 0.9984 for specific and total IgE respectively. ImmunoCap 250 is a good solution for laboratories that perform twenty to thirty thousand determinations. [less ▲]

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See detailIntérêt de la spectrométrie de mobilité ionique pour l'étude de la conformation et des assemblages non-covalents de biomolécules
Gabelica, Valérie ULg

in Spectra Analyse (2006), 251

This paper gives an overview of experimental and theoretical aspects of the ion mobility spectrometry technique coupled to mass spectrometry. These aspects are illustrated by a recent study on the ... [more ▼]

This paper gives an overview of experimental and theoretical aspects of the ion mobility spectrometry technique coupled to mass spectrometry. These aspects are illustrated by a recent study on the conformation of a DNA sequence capable of forming intramolecular G-quadruplex folds. This sequence is found in the promoter of the gene coding for the c-Myc protein. The comparison between experimental collision cross sections determined by ion mobility spectrometry and theoretical cross sections calculated for several model structures allows demonstrating that ions of low charge states have conserved the G-quadruplex parallel fold that was present in solution. Mass spectrometry also revealed the presence of a dimer, which conformation was interpreted using ion mobility : this structure consists of two parallel monomers stacked with each other. [less ▲]

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See detailQuelques réflexions sur la lex societatis dans le Code de droit international privé
Wautelet, Patrick ULg

in Revue Pratique des Sociétés Civiles et Commerciales (2006)

L'article fait le point sur le nouveau régime des sociétés en droit international tel qu'introduit par le Code de droit international privé belge. Après un examen des questions classiques liées à la ... [more ▼]

L'article fait le point sur le nouveau régime des sociétés en droit international tel qu'introduit par le Code de droit international privé belge. Après un examen des questions classiques liées à la détermination du droit applicable aux personnes morales, dont notamment la question de la nationalité des personnes morales ainsi que celle de la reconnaissance, l'article s'attarde sur la nouvelle règle de rattachement retenu par le législateur belge, pour examiner si elle répond aux nombreuses critiques formulées par les partisans de la doctrine de l'incorporation. [less ▲]

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See detailThe Photoabsorption and Photoionization of Bromine revisited.
Locht, Robert ULg; Leyh, Bernard ULg; Jochims, H.-W. et al

Book published by Berliner Elektronen Speicherring für Synchrotron Strahlund m.b.H - on CD-ROM (2006)

The V-UV photoabsorption spectrum (PAS) and the photoionization (PIE) efficiency of Br2, Br2+ and Br+ have been measured using synchrotron radiation. At high photon energy a strong correlation between the ... [more ▼]

The V-UV photoabsorption spectrum (PAS) and the photoionization (PIE) efficiency of Br2, Br2+ and Br+ have been measured using synchrotron radiation. At high photon energy a strong correlation between the PAS of Br2 and the PIE of Br2+ is observed. A detailed analysis of the PIE of Br2+ in the threshold region is presented. New data related to the Br+/Br- ion-pair formation are reported. [less ▲]

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See detailThe VUV Photoabsorption Spectroscopy and Photoionization of Halogenated Etylenes: Bromo-derivatives.
Locht, Robert ULg; Leyh, Bernard ULg; Jochims, H.-W. et al

Book published by Berliner Elektronen Speicherring für Synchrotron Strahlung m.b.H - on CD-ROM (2006)

In this contribution the measurement, by the use of synchrotron radiation, of the V-UV photoabsorption spectrum (PAS) of 1,1-C2H2Br2 and the photoionization efficiency curve (PIC) of 1,1-C2H2Br2+ are ... [more ▼]

In this contribution the measurement, by the use of synchrotron radiation, of the V-UV photoabsorption spectrum (PAS) of 1,1-C2H2Br2 and the photoionization efficiency curve (PIC) of 1,1-C2H2Br2+ are reported. Strong correlations between the PAS and the PIC are shown. The PAS of 1,1-C2F2Br2 is also presented and compared to the PAS of 1,1-C2H2Br2 to show the influence of the fluuorine-substitution. [less ▲]

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See detailAux confins de la norme : quelques réflexions sur le statut du droit étranger en droit international privé belge
Wautelet, Patrick ULg

in Pâques, Michel (Ed.) Liber Liber amicorum Paul Martens. L'humanisme dans la résolution des conflits. Utopie ou réalité? (2006)

In this article, an attempt is made to describe the status of foreign law in Belgian private international law. The focus is in particular on the difference in the relationship between the court and the ... [more ▼]

In this article, an attempt is made to describe the status of foreign law in Belgian private international law. The focus is in particular on the difference in the relationship between the court and the legal rule which is applied to solve the dispute, depending on whether the rule is a local rule or a foreign rule. [less ▲]

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See detailSymlinks as boundary objects
Lejeune, Christophe ULg

in TeamEthno-Online (2006), 2

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See detailConflits et territorialisation
Mormont, Marc ULg

in Géographie Economie Sociétés (2006), 8(3), 399-418

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