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See detailFactors affecting hematopoietic recovery after autologous peripheral blood progenitof-cell transplantation in aggressive non-Hodgkin's lymphoma : a prospective study of 123 patients
Langouet, Anne-Marie; Brice, Pauline; Simon, Danielle et al

in Hematology Journal : The Official Journal of the European Haematology Association (2001), 2

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See detailLes Amériques du jeune Turgot
Droixhe, Daniel ULg

in Van Crugten-André, Valérie (Ed.) Vérité et littérature au XVIIIe siècle : mélanges rassemblés en l'honneur de Raymond Trousson (2001)

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See detailCaractérisation de particules d'engrais : corrélation entre les propriétés physiques et morphométriques
Miserque, Olivier; Tissot, Serge; Pirard, Eric ULg

in Récents Progrès en Génie des Procédés (2001), 15(78),

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See detailMultispectral Imaging in Cathodoluminescence Microscopy
Charlier, Bernard ULg; De Colnet, Laurence; Pirard, Eric ULg

in Proceedings - Annual Meeting - Belgian Soc. Microscopy (2001)

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See detailLe concept d'insulinosensibilite
Scheen, André ULg

in Diabètes & Métabolism (2001), 27(2 Pt 2), 193-200

Insulin resistance was already suspected in the thirties from clinical observations in diabetic patients, then better appreciated in the early sixties with the development of insulin radioimmunoassay, and ... [more ▼]

Insulin resistance was already suspected in the thirties from clinical observations in diabetic patients, then better appreciated in the early sixties with the development of insulin radioimmunoassay, and finally confirmed in the last 20 years by using various sophisticated methods able to quantify insulin action. First demonstrated in obese and/or type 2 diabetic patients, the diminution of insulin sensitivity may in fact concern a much larger population. The concept of insulin sensitivity gained a considerable importance when Reaven, in 1988, emphasized the role of insulin resistance in different human diseases. The metabolic syndrome or syndrome X is characterized by the association of various cardiovascular risk factors (among which impaired glucose tolerance, arterial hypertension and dyslipidaemias), all closely linked to insulin resistance which is indeed the core of the syndrome. Even if insulin action signalling appears to be rather complex and the mechanisms leading to insulin resistance still largely unknown, it is essential to develop pharmacological or non pharmacological strategies to improve insulin sensitivity for treating insulin-resistant individuals. Such an approach should allow not only to provide a better blood glucose control in patients with type 2 diabetes, but also to improve the cardiovascular prognosis of numerous patients, with or without diabetes mellitus, who have the metabolic syndrome. [less ▲]

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See detailGranulométrie et Morphométrie des matériaux granulaires en laboratoire
Pirard, Eric ULg

in Techniques de l'Industrie Minérale (2001), 12

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See detailThiazolidinediones and liver toxicity.
Scheen, André ULg

in Diabètes & Métabolism (2001), 27(3), 305-13

Thiazolidinediones or glitazones specifically target insulin resistance. They have proven efficacy for reducing plasma glucose levels of type 2 diabetic patients treated with diet alone, sulphonylureas ... [more ▼]

Thiazolidinediones or glitazones specifically target insulin resistance. They have proven efficacy for reducing plasma glucose levels of type 2 diabetic patients treated with diet alone, sulphonylureas, metformin or insulin. In addition, they may be associated to some improvement of cardiovascular risk profile. However, troglitazone, the first compound approved by the FDA in the US, proved to be hepatotoxic and was withdrawn from the market after the report of several dozens of deaths or cases of severe hepatic failure requiring liver transplantation. It remains unclear whether or not hepatotoxicity is a class effect or is related to the unique tocopherol side chain of troglitazone. Rosiglitazone and pioglitazone, two other glitazones, appear to have similar efficacy on blood glucose control of type 2 diabetic patients as compared to troglitazone. In controlled clinical trials, the incidence of significant increases in liver enzyme levels (ALT) was similar with rosiglitazone or pioglitazone as compared to placebo, whereas troglitazone was associated with a threefold greater incidence. In contrast to the numerous case reports of acute liver failure in patients receiving troglitzone, only two cases of severe reversible liver failure have been reported in patients treated with rosiglitazone, with a causal relationship remaining uncertain. Furthermore, no single case of severe hepatotoxicity has been reported yet with pioglitazone. While regular monitoring of liver enzymes is still recommended and more long-term data are desirable, current clinical evidence supports the conclusion that rosiglitazone and pioglitazone do not share the hepatotoxic profile of troglitazone. [less ▲]

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See detailApplication and processing of geophysical images for mapping faults
Demanet, Donat; Renardy, François; Pirard, Eric ULg et al

in Computers & Geosciences (2001), 29(7), 1031-1037

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See detailMultiradial Imaging in Optical Ore Microscopy
Pirard, Eric ULg; De Colnet, Laurence

in Proceedings - Annual Meeting - Belgian Soc. Microscopy (2001)

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See detailGeological application of digital imaging
Pirard, Eric ULg; Jongmans, Denis; Marsh, Stuart

in Computers & Geosciences (2001), 27(9), 1015-1017

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See detailOn the Use of Weak Automata for Deciding Linear Arithmetic with Integer and Real Variables
Boigelot, Bernard ULg; Jodogne, Sébastien ULg; Wolper, Pierre ULg

in Lecture Notes in Computer Science (2001), 2083

This paper considers finite-automata based algorithms for handling linear arithmetic with both real and integer variables. Previous work has shown that this theory can be dealt with by using finite ... [more ▼]

This paper considers finite-automata based algorithms for handling linear arithmetic with both real and integer variables. Previous work has shown that this theory can be dealt with by using finite automata on in finite words, but this involves some difficult and delicate to implement algorithms. The contribution of this paper is to show, using topological arguments, that only a restricted class of automata on in finite words are necessary for handling real and integer linear arithmetic. This allows the use of substantially simpler algorithms and opens the path to the implementation of a usable system for handling this combined theory. [less ▲]

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See detailBeta-lactamase inhibitors derived from single-domain antibody fragments elicited in the camelidae.
Conrath, K. E.; Lauwereys, M.; Galleni, Moreno ULg et al

in Antimicrobial Agents and Chemotherapy (2001), 45(10), 2807-12

Small, soluble single-domain fragments derived from the unique variable region of dromedary heavy-chain antibodies (VHHs) against enzymes are known to be potent inhibitors. The immunization of dromedaries ... [more ▼]

Small, soluble single-domain fragments derived from the unique variable region of dromedary heavy-chain antibodies (VHHs) against enzymes are known to be potent inhibitors. The immunization of dromedaries with the TEM-1 and BcII beta-lactamases has lead to the isolation of such single-domain antibody fragments specifically recognizing and inhibiting those beta-lactamases. Two VHHs were isolated that inhibit TEM-1 and one BcII inhibiting VHH was identified. All inhibitory VHHs were tight-binding inhibitors. The 50% inhibitory concentrations were determined for all inhibitors and they were all in the same range as the enzyme concentration used in the assay. Addition of the VHHs to the TEM-1 beta-lactamase, expressed on the surface of bacteria, leads to a higher ampicillin sensitivity of the bacteria. This innovative strategy could generate multiple potent inhibitors for all types of beta-lactamases. [less ▲]

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See detailS179D-human PRL, a pseudophosphorylated human PRL analog, is an agonist and not an antagonist
Bernichtein, Sophie; Kinet, Sandrina; Jeay, Sébastien et al

in Endocrinology (2001), 142(9), 3950-63

For many years, our group has been involved in the development of human PRL antagonists. In two recent publications, S179D-human PRL, a human PRL analog designed to mimic a putative S179-phosphorylated ... [more ▼]

For many years, our group has been involved in the development of human PRL antagonists. In two recent publications, S179D-human PRL, a human PRL analog designed to mimic a putative S179-phosphorylated human PRL, was reported to be a highly potent antagonist of human PRL-induced proliferation and signaling in rat Nb2 cells. We prepared this analog with the aim of testing it in various bioassays involving the homologous, human PRL receptor. In our hands, S179D- human PRL was able to stimulate 1) the proliferation of rat Nb2 cells and of human mammary tumor epithelial cells (T-47D), 2) transcriptional activation of the lactogenic hormone response element-luciferase reporter gene, and 3) activation of the Janus kinase/signal transducer and activator of transcription and MAPK pathways. Using the previously characterized antagonist G129R-human PRL as a control, we failed to observe any evidence for antagonism of S179D-human PRL toward any of the human PRL-induced effects analyzed, including cell proliferation, transcriptional activation, and signaling. In conclusion, our data argue that S179D-human PRL is an agonist displaying slightly reduced affinity and activity due to local alteration of receptor binding site 1, and that the antagonistic properties previously attributed to S179D-human PRL cannot be confirmed in any of the assays analyzed in this study. [less ▲]

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See detailCharacterization of the human Fc gamma RIIB gene promoter: human zinc-finger proteins (ZNF140 and ZNF91) that bind to different regions function as transcription repressors
Nishimura, Tadahiro; Narita, Tadashi; Miyazaki, Emi et al

in International Immunology (2001), 13(8), 1075-84

Expression of the human low-affinity Fc receptors for IgG (human Fc gamma RII) is differentially regulated. We report here the characterization of the promoter structure of the human Fc gamma RIIB gene ... [more ▼]

Expression of the human low-affinity Fc receptors for IgG (human Fc gamma RII) is differentially regulated. We report here the characterization of the promoter structure of the human Fc gamma RIIB gene and the isolation of the promoter region-binding proteins by a yeast one-hybrid assay. The minimal 154-bp region upstream from the transcription start site of the human Fc gamma RIIB gene was shown to possess promoter activity in a variety of cells. An electrophoretic mobility shift assay indicated that multiple nuclear factors in cell extracts bind to the two regions [F2-3 (-110 to -93) and F4-3 (-47 to -31)] of the human Fc gamma RIIB gene promoter. Mutation analysis indicated that GGGAGGAGC (-105 to -97) and AATTTGTTTGCC (-47 to -36) sequences are responsible for binding to nuclear factors respectively. By using GGGAGGAGC and AATTTGTTTGCC as bait sequences, we cloned two zinc-finger proteins (ZNF140 and ZNF91) that bind to the F2-3 and F4-3 regions within the promoter of the human Fc gamma RIIB gene respectively. When the ZNF140 and ZNF91 were transfected with reporter plasmid, both showed repressor activity with additive effects. Thus, these results indicate that these cloned ZNF140 and ZNF91 proteins function as repressors for the human Fc gamma RIIB transcription. [less ▲]

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See detailComparison between Solution-Phase Stability and Gas-Phase Kinetic Stability of Oligodeoxynucleotide Duplexes
Gabelica, Valérie ULg; De Pauw, Edwin ULg

in Journal of Mass Spectrometry [=JMS] (2001), 36(4), 397-402

The relative kinetic stabilities of different 16-mer oligonucleotide duplexes were investigated by source collision-induced dissociation (CID) in a heated capillary electrospray ion source. They were ... [more ▼]

The relative kinetic stabilities of different 16-mer oligonucleotide duplexes were investigated by source collision-induced dissociation (CID) in a heated capillary electrospray ion source. They were compared with the relative stabilities in solution obtained by thermal denaturation monitored by UV spectrophotometry. The results clearly show that both hydrogen bonding and base stacking interactions that are present in solution are maintained in the gas phase. This suggests that the electrospray process preserves the double-helix structure of DNA. A step by step opening of the double helix structure is proposed for the gas-phase dissociation, competing with the covalent bond cleavage of bases. We also draw attention to the fact that by source CID, it is the kinetic stability of the complexes that is probed. In particular, this implies that only complexes of the same size can be compared. [less ▲]

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See detailKinetics of solvent addition on electrosprayed ions in an electrospray source and in a quadrupole ion trap
Gabelica, Valérie ULg; Lemaire, David; Laprévote, Olivier et al

in International Journal of Mass Spectrometry (2001), 210/211

Benzylpyridinium cations readily fragment in the electrospray source by loss of pyridine to give benzyl cations (M-79). The full-scan spectra obtained with some instruments also show, in addition, an m/z ... [more ▼]

Benzylpyridinium cations readily fragment in the electrospray source by loss of pyridine to give benzyl cations (M-79). The full-scan spectra obtained with some instruments also show, in addition, an m/z (M-38) peak corresponding to the addition of acetonitrile, being present in the solvent mixture, on the benzyl cations. Here we report that the addition reaction can occur in the source region of electrospray mass spectrometry instruments, and in a quadrupole ion trap. The kinetics of acetonitrile addition was monitored in an ion trap, acetonitrile being provided by leakage from the source, through the heated capillary. For benzyl ions with different substituents, the addition kinetics has been found positively correlated with the Brown parameter sigma+ of the benzyl radical, and therefore with the effective charge density on the alpha-carbon atom of the benzyl ion. This is consistent with the Langevin or average-dipole-orientation (ADO) theory of ion–molecule reaction kinetics. [less ▲]

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See detailQUESTIONS SPECIALES DE TECHNOLOGIE
Debongnie, Jean-François ULg

Book published by DEBONGNIE (JEAN-FRANCOIS) (2001)

Analytical solutions of the rigid-plastic model to forming processes

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See detailChemometrics methods for the identification and the monitoring of an odour in the environement with an electronic nose
Nicolas, Jacques ULg; Romain, Anne-Claude ULg; Maternova, J.

in Ramirez-Silva, Maria Theresa (Ed.) Sensors and chemometrics (2001)

The purpose of the paper is to briefly review some researches regarding the adaptation of the electronic nose principle to recognise some malodour sources in the environment, if possible directly in the ... [more ▼]

The purpose of the paper is to briefly review some researches regarding the adaptation of the electronic nose principle to recognise some malodour sources in the environment, if possible directly in the field, and to monitor the odour intensity continuously. Research aims at improving the portability and the user-friendliness of the instrument, together with testing what kind of signal may be used to monitor the odour. A laboratory-made electronic nose, constituted of an array of tin-oxide sensors, is used in different configurations. The ambient air is either sampled around environmental sources (landfill, urban waste composting facilities, …), or directly transferred into the sensor chamber in the field. Two main options are considered : firstly, identifying the source of odour in the background and among interfering odours and, secondly, when the malodour is recognised, trying to monitor it continuously in order, for example, to assess the nuisance or to control an odour abatement system. Chemometrics methods are generally used for both purposes. They provide quick answers and allow to evaluate the relationships between variables and between observations at a glance. They are applied on the sensor signals, eventually preprocessed by a suitable algorithm. Non-supervised analyses, such as Principal Component Analysis (PCA), provide basically a performance evaluation of the system during the development phase. On the contrary, supervised analyses, such as Discriminant Analysis (DA), or some Neural Networks algorithms are quite appropriate to make a reliable recognition in real time, when the system is developed. To predict the odour intensity, different techniques are tested : either using only one of the sensor elements, or applying different chemometrics techniques, such as Multilinear Regression (MLR) on the original measured sensor signals, Principal Component Regression (PCR), or Partial Least Squares regression (PLS). The latter seems to be the most adapted model for the intensity prediction. [less ▲]

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See detailAn approach to modal analysis of power system angle stability
Roth, Anna; Ruiz-Vega, Daniel; Ernst, Damien ULg et al

in Proceedings of IEEE Powertech 2001 (2001)

An approach to modal analysis and modal identification is proposed, capable of complementing the panoply of existing methods. It is based on a hybrid time-domain – direct transient stability method called ... [more ▼]

An approach to modal analysis and modal identification is proposed, capable of complementing the panoply of existing methods. It is based on a hybrid time-domain – direct transient stability method called SIME (for SIngle Machine Equivalent). In short, SIME uses a conventional transient stability program to transform the time varying parameters of the system into those of a one-machine infinite bus (OMIB) equivalent system. The representations of this OMIB allow substantial reduction of the original problem’s dimensionality. Many important advantages may result. For example, the multimachine system unstable equilibrium point (UEP) can readily be derived from the OMIB UEP, which is calculated analytically and unambiguously in a two-dimensional space. Further, the interplay between multimachine and OMIB characteristics and their complementary properties provides a better understanding and handling of damping, inter-area oscillations and their control. More generally, modal analysis and modal identification tasks get closer to each other and easier to handle. The paper essentially focuses on the approach as such rather than on potential applications. Simulations carried out on a 3-machine system illustrate main features. [less ▲]

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