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See detailRedefining the role of psr in beta-lactam resistance and cell autolysis of Enterococcus hirae.
Sapunaric, Frédéric ULg; Franssen, Christine; Stefanic, Patrick et al

in Journal of Bacteriology (2003), 185(20), 5925-35

The contribution of penicillin-binding protein 5 (PBP5) and the PBP5 synthesis repressor (Psr) to the beta-lactam resistance, growth, and cell autolysis of wild-type strain ATCC 9790 and resistant strain ... [more ▼]

The contribution of penicillin-binding protein 5 (PBP5) and the PBP5 synthesis repressor (Psr) to the beta-lactam resistance, growth, and cell autolysis of wild-type strain ATCC 9790 and resistant strain R40 of Enterococcus hirae was investigated by disruption or substitution of the corresponding pbp5 and psr genes by Campbell-type recombination. The resulting modifications were confirmed by hybridization and PCR. The low susceptibility of E. hirae to beta-lactams was confirmed to be largely dependent on the presence of PBP5. However, against all expectations, inactivation of psr in ATCC 9790 or complementation of R40 cells with psr did not modify the susceptibility to benzylpenicillin or the growth and cell autolysis rates. These results indicated that the psr gene does not seem to be involved in the regulation of PBP5 synthesis and consequently in beta-lactam resistance or in the regulation of cell autolysis in E. hirae. [less ▲]

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See detailAfrique de l'Ouest: commerce d'oiseaux pas comme les autres
Libois, Roland ULg; Lougbégnon, Olou

Learning material (2003)

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See detailMycoplasma bovis : synthèse des connaissances actuelles
Thomas, Anne; Mainil, Jacques ULg; Linden, Annick ULg

in Annales de Médecine Vétérinaire (2003), 147(1, FEB-MAR), 23-39

Mycoplasmas frequently infect cattle. Amongst them, M. Bovis is the most pathogenic species in countries free from contagious bovine pleuropneumonia because it is responsible for bronchopneumonia ... [more ▼]

Mycoplasmas frequently infect cattle. Amongst them, M. Bovis is the most pathogenic species in countries free from contagious bovine pleuropneumonia because it is responsible for bronchopneumonia, arthritis and mastitis, and is thus associated with strong economic losses. Several studies have shown the frequency of M. bovis in Europe and the spread of antibiotic-resistant strains. Considering the absence of vaccine in Europe, it is essential to understand this bacteria in order to control the infection in cattle. In this context, this paper aims at summarizing the current knowledge about M. bovis. [less ▲]

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See detailHistological study of immature interspecific embryo abortion between Phaseolus vulgaris L. and P. polyanthus Greenm.
Toussaint, André ULg; Geerts, P.; Mergeai, Guy ULg et al

in Annual Report of the Bean Improvment Cooperative (2003), 46

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See detailMycoplasma bovis dans le complexe respiratoire bovin et propriétés de cyto-adhésion in vitro
Thomas, Anne; Dizier, Isabelle ULg; Sachse, K. et al

in Annales de Médecine Vétérinaire (2003), 147(4, AUG-SEP), 267-272

Mycoplasmas frequently infect cattle, causing especially respiratory diseases. Mycoplasma bovis is the most important pathogenic species in countries free of bovine contagious pleuropneumonia. This ... [more ▼]

Mycoplasmas frequently infect cattle, causing especially respiratory diseases. Mycoplasma bovis is the most important pathogenic species in countries free of bovine contagious pleuropneumonia. This species was frequently isolated in Belgium from cattle with respiratory disease. Furthermore, associations were often observed with pasteurellas and bovine respiratory syncytial virus. Of these M. bovis isolates, many were resistant to several antimicrobial agents which are used in cattle practice, except to fluoroquinolones. Inasmuch the high frequency of M. bovis isolation and antibiotic resistances, it is very important to understand the pathogenicity of this bacteria in order to optimize prophylactic tools. Therefore, the study of the cytadherence of M. bovis is essential since it represents the first step of the bacterial infection. According to our experimental results, PG45 is not representative of field isolates because of its low adherence rates to various cell lines. This could be explained by the high number of subcultures of this pathogenic strain underwent since its first isolation, which sharply contrasts with other isolates. M. bovis adheres specifically to bovine bronchial epithelial cells in primary culture. Proteins such as variable surface proteins C and F are involved in this step as observed by decreased adherence rates after trypsinization of mycoplasma cells or addition of monoclonal antibodies. [less ▲]

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See detailEvolution of pressure distribution during apple compression tests measured with tactile sensors
Kleynen, Olivier; de la Cierva, Sonia; Destain, Marie-France ULg

in Acta Horticulturae (2003), (604),

The paper analyses the ability of thin-film tactile sensors in providing information during static compression tests of ‘Jonagold’ apples (Malus pumila) of different ripeness stages. Such sensors are able ... [more ▼]

The paper analyses the ability of thin-film tactile sensors in providing information during static compression tests of ‘Jonagold’ apples (Malus pumila) of different ripeness stages. Such sensors are able to measure the contact surface and the interfacial pressure distribution during compression of fruits, this latter being characterised by suitable mathematical parameters. Results of compression tests between two flat steel plates are presented. The differentiated evolution of the pressure distribution according to the fruit maturity is pointed out. Ability of the sensor in evaluating the firmness is also discussed. [less ▲]

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See detailBehavioral characterization of acetaldehyde in C57BL/6J mice: Anxiolytic, amnesic and hedonic effects
Tambour, Sophie ULg; Quertemont, Etienne ULg; Tirelli, Ezio ULg

in Behavioural Pharmacology (2003), 14(Suppl. 1), 68-69

It has been postulated that a number of central effects of ethanol are mediated through the action of its first metabolite, acetaldehyde. In particular, acetaldehyde might be involved in the anxiolytic ... [more ▼]

It has been postulated that a number of central effects of ethanol are mediated through the action of its first metabolite, acetaldehyde. In particular, acetaldehyde might be involved in the anxiolytic and hedonic effects of ethanol and is therefore believed to play an important role in alcohol abuse. In agreement with this assumption, previous studies indicated that acetaldehyde is mainly reinforcing in rats, which have been shown to readily self-administer acetaldehyde both peripherally and centrally. However, the hedonic effects of acetaldehyde have never been tested in mice, and the possible amnesic and anxiolytic effects of acetaldehyde remain to be elucidated. Therefore, the present studies were aimed at characterizing the anxiolytic, hedonic and amnesic effects of acetaldehyde after its acute peripheral administration to C57BL/6J mice. The effects of intraperitoneal acetaldehyde (0-300 mg/kg) injections were assessed in several classical behavioral tests. The anxiolytic effects were tested with the elevated plus maze, the hedonic effects with the place conditioning procedure and the amnesic effects with the passive avoidance apparatus. Our results show that acetaldehyde dose-dependently altered memory consolidation as evidenced by a reduced performance in the passive avoidance test when acetaldehyde was injected immediately after training at doses between 100 and 300 mg/kg. The elevated plus-maze showed that acetaldehyde, in contrast to ethanol, does not possess anxiolytic properties. Finally, the results of the place conditioning experiment confirmed that acetaldehyde displays significant hedonic properties. The present results add further support to the role of acetaldehyde in ethanol amnesic and hedonic effects but interestingly suggest that acetaldehyde is not involved in ethanol anxiolytic effects. [less ▲]

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See detailBehavioral characterization of acetaldehyde in C57BL/6J mice: Locomotor, hypnotic and ataxic effects
Quertemont, Etienne ULg; Tambour, Sophie ULg; Tirelli, Ezio ULg

in Behavioural Pharmacology (2003), 14(Suppl. 1), 69-69

Acetaldehyde, the first ethanol metabolite, was recently suggested to play a major role in many behavioral effects of ethanol. However, no studies have directly investigated the behavioral effects of ... [more ▼]

Acetaldehyde, the first ethanol metabolite, was recently suggested to play a major role in many behavioral effects of ethanol. However, no studies have directly investigated the behavioral effects of acetaldehyde after acute administration. Therefore, the aim of the present study was to characterize the locomotor, hypnotic and ataxic effects of acetaldehyde in C57BL/6J mice. Various acetaldehyde doses (0-300 mg/kg) were injected intraperitoneally and their effects were investigated with several classical behavioral tests. The locomotor effects of acetaldehyde were measured in standard activity boxes. In addition, the loss of righting reflex was used to assess the hypnotic effects of acetaldehyde. Finally, the ataxic effects of acetaldehyde were studied with the horizontal wire test. The results show that acetaldehyde induced a significant hypolocomotor effect at 170 mg/kg and higher doses. In addition, the hypnotic effects of acetaldehyde were evidenced by a loss of righting reflex in doses between 170 and 300 mg/kg. However, the locomotor and hypnotic effects of acetaldehyde were very brief relative to what is observed after ethanol administration. After 170 mg/kg acetaldehyde, normal activity was recovered in less than 30 minutes and the loss of righting reflex lasted only an average of 6.14 ± 1.29 minutes after the administration of 300 mg/kg acetaldehyde, the highest testable dose before lethality. Ataxic effects were observed with lower doses that did not significantly affect locomotor activity. These results show that acetaldehyde, like ethanol, possesses sedative, hypnotic and ataxic properties and therefore indicate that the first product of ethanol metabolism might be involved in these ethanol effects. [less ▲]

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See detailL'idiologue à raison : A propos du P. Cattaoui, recto, col. V = M. Chr. 372, col. VI
Straus, Jean ULg

in Chronique d'Egypte : Bulletin Périodique de la Fondation Egyptologique Reine Elisabeth (2003), LXXVIII(155-156), 217-220

Examination of the minutes of a hearing before the idios logos (M. Chr. 372; 22 November A.D. 136) in which the ownership of slaves left by a deceased soldier without heirs poses problems. The solution ... [more ▼]

Examination of the minutes of a hearing before the idios logos (M. Chr. 372; 22 November A.D. 136) in which the ownership of slaves left by a deceased soldier without heirs poses problems. The solution chosen by the idios logos rests on a sound logic and a fair application of the law.. [less ▲]

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See detailMarine mammals from Northeast Atlantic: relationship between their trophic status as determined by delta C-13 and delta N-15 measurements and their trace metal concentrations
Das, Krishna ULg; Beans, Cristina; Holsbeek, Ludo et al

in Marine Environmental Research (2003), 56(3),

The relationship between trophic position through delta(13)C and delta(15)N and trace metal concentrations (Zn, Cd, Cu and Hg) was investigated in the tissues of six marine mammal species from the ... [more ▼]

The relationship between trophic position through delta(13)C and delta(15)N and trace metal concentrations (Zn, Cd, Cu and Hg) was investigated in the tissues of six marine mammal species from the Northeast Atlantic: striped dolphin Stenella coeruleoalba, common dolphin, Delphinus delphis. Atlantic white-sided dolphin Lagenorhynchus acutus, harbour porpoise Phocoena phocoena, white beaked-dolphin Lagenorhynchus albirostris, grey seal Halichoerus grypus stranded on French Channel and Irish coasts. White-beaked dolphins, harbour porpoises, white-sided dolphins, common and striped dolphins display the same relative and decreasing trophic position, as measured by delta(15)N values, along both the Irish and French channel coasts, reflecting conservative trophic habits between these two places. Hepatic and renal Cd concentrations were significantly correlated to muscle delta(13)C and delta(15)N values while Hg, Zn and Cu did not. These results suggest that Cd accumulation is partly linked to the diet while other factors Such as age or body condition might explain Hg, Zn or Cu variability in marine mammals. Combined stable isotope and trace metal analyses appear to be useful tools for the study of marine mammal ecology. (C) 2003 Elsevier Science Ltd. All rights reserved. [less ▲]

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See detailLaudatio Hubert Nyssen
Durand, Pascal ULg

Speech (2003)

Présentation de la double carrière d'Hubert Nyssen écrivain et éditeur à l'occasion de son doctorat honoris causa à l'Université de Liège

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See detailHet vrij verkeer van beslissingen binnen de Europese Unie : toepassing op het arbeidsrechtelijk contentieux
Wautelet, Patrick ULg

in de Leval, Georges (Ed.) Espace judiciaire et social européen (2003)

In this paper, written with Prof. Marta Pertegás Sender, we give an overview of the achievements made within the EU in securing the mutual recognition and enforcement of court decisions issued in ... [more ▼]

In this paper, written with Prof. Marta Pertegás Sender, we give an overview of the achievements made within the EU in securing the mutual recognition and enforcement of court decisions issued in employment disputes. Particular attention is given to the special regime of employment disputes under the 1968 Brussels Convention and the Regulation 44/2001. [less ▲]

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See detailLe trésor de reliques du Neufmoustier près de Huy. Une part de Terre sainte en pays mosan
George, Philippe ULg

in Bulletin de la Commission Royale d'Histoire (2003)

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See detailTrophic habits and aquatic microhabitat use in gilled immature, paedomorphic and metamorphic Alpine newts (Triturus alpestris apuanus) in a pond in central Italy
Denoël, Mathieu ULg; Andreone, F.

in Belgian Journal of Zoology (2003), 133(2), 95-102

Current evolutionary models suggest that the presence of heterogeneous habitats favours the evolution of polymorphisms. In such cases, alternative phenotypes can coexist because they use different ... [more ▼]

Current evolutionary models suggest that the presence of heterogeneous habitats favours the evolution of polymorphisms. In such cases, alternative phenotypes can coexist because they use different resources. Facultative paedomorphosis is a heterochronic polymorphism in which a morph - the paedomorph - retains larval traits during the adult stage while the other morph - the metamorph - is fully metamorphosed. The aim of this study was to determine the microhabitat use and the diet of Alpine newt paedomorphs, metamorphs and immatures (Triturus alpestris apuanus) coexisting in a small pond in Tuscany, central Italy, i.e. in a habitat where dimorphism is not expected. Although the two adult morphs do not use exactly the same resources, resource partitioning was weaker than in deep Alpine lakes. Nevertheless, the diet of immature gilled newts (larvae) differed from that of adults (metamorphs and paedomorphs). While the larvae eat a large number of planktonic organisms, the adults focus on insect larvae and newt eggs. The differences in resource use favour the coexistence of aquatic juveniles and adults. In the studied pond, facultative paedomorphosis was previously shown to be favoured by a precocious maturity of the paedomorphs. This study shows that the coexistence of paedomorphs and metamorphs may also be supported by some dietary and spatial segregation, although any advantages gained by this pattern are rather limited in the adult stage. [less ▲]

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See detailGuided vortex motion in superconductors with a square antidot array
Silhanek, Alejandro ULg; Van Look, L.; Raedts, S. et al

in Physical Review. B : Condensed Matter (2003), 68(21),

We have measured the in-plane anisotropy of the vortex mobility in a thin Pb film with a square array of antidots. The Lorentz force, acting on the vortices, was rotated by adding two perpendicular ... [more ▼]

We have measured the in-plane anisotropy of the vortex mobility in a thin Pb film with a square array of antidots. The Lorentz force, acting on the vortices, was rotated by adding two perpendicular currents and keeping the amplitude of the net current constant. One set of voltage probes was used to detect the vortex motion. We show that the pinning landscape provided by the square antidot lattice influences the vortex motion in two different ways. First, the modulus of the vortex velocity becomes angular dependent with a lower mobility along the diagonals of the pinning array. Second, the vortex displacement is preferentially parallel to the principal axes of the underlying pinning lattice, giving rise to a misalignment between the vortex velocity and the applied Lorentz force. We show that this anisotropic vortex motion is temperature dependent and progressively fades out when approaching the normal state. [less ▲]

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See detailCultures maraîchères et insectes utiles
Francis, Frédéric ULg; Colignon, Pierre; Haubruge, Eric ULg

in Le Canard déchaîné du Kauwberg (2003), 46

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See detailQuelle formation andragogique pour les enseignants universitaires demain ?
Schmitz, Serge ULg

in Actes du Geoforum (2003), 27

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See detailProduits Fermiers : entre Qualification et Identité
Stassart, Pierre M ULg

Book published by Presses Interuniversitaires Européennes (2003)

Cet ouvrage propose une première «écologie» de la qualité alimentaire. Dans ce domaine, la qualité implique l'intervention de multiples agents naturels, techniques et humains. Comment construisent-ils une ... [more ▼]

Cet ouvrage propose une première «écologie» de la qualité alimentaire. Dans ce domaine, la qualité implique l'intervention de multiples agents naturels, techniques et humains. Comment construisent-ils une définition stable et crédible de la qualité? Cette question est traitée à travers l'analyse d'un projet paysan de type coopératif. Son évolution suite à la rencontre des consommateurs et des transformateurs permet de comprendre pourquoi il s'organise et se différencie autour d'un produit critique: la viande. L'analyse ne se limite pas ici aux produits fermiers. Elle se confronte au secteur de la viande pour éclairer ainsi les processus de formation des «intermédiaires» dans l'allongement de la filière. La question de la crédibilité nous force à déplacer le regard de la qualification du produit vers ceux qui le produisent, vers leurs engagements face aux consommateurs. Ce sont ces engagements qui permettent de gérer le risque alimentaire. [less ▲]

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