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See detailBronchoconstrictive properties of inhaled 8-epi-Pgf2alpha in healthy and heaves-susceptible horses
Kirschvink, Nathalie; Bureau, Fabrice ULg; Art, Tatiana ULg et al

in Veterinary Research (2001), 32(5, Sep-Oct), 397-407

The 8-epi-PGF2α is a marker of oxidative stress which is increased in lungs of asthmatic humans and heaves-susceptible horses. 8-Epi-PGF2α has also been demonstrated to be an in vitro and in vivo ... [more ▼]

The 8-epi-PGF2α is a marker of oxidative stress which is increased in lungs of asthmatic humans and heaves-susceptible horses. 8-Epi-PGF2α has also been demonstrated to be an in vitro and in vivo bronchoconstrictor in humans and rodents. We hypothesised that inhaled 8-epi-PGF2α was a bronchoconstrictor in healthy and heaves-susceptible horses in clinical remission. The effect on ventilatory mechanics of nebulised 8-epi-PGF2α was compared to that of PGF2α and U46619, a thromboxane A2 agonist. Pulmonary resistance (RL) and dynamic compliance (Cdyn) were assessed in six healthy horses and in six heaves-susceptible horses in clinical remission before (baseline) and immediately after a single inhalation challenge of 1 mg 8-epi-PGF2α, PGF2α, or U46619 and placebo. RL and Cdyn were unchanged after inhalation of 8-epi-PGF2α in healthy horses. In heaves-susceptible horses, 8-epi-PGF2α induced a significant increase of RL and a significant decrease of Cdyn when compared to baseline values. Differences between RL and Cdyn values after 8-epi-PGF2α inhalation and those of placebo inhalation were not significant. Differences with healthy horses were not significant. PGF2α and U46619 induced a significant bronchoconstriction in healthy (RL and Cdyn, versus baseline) and heaves-susceptible horses (RL and Cdyn, versus baseline and placebo). The RL increase in heaves-susceptible horses after PGF2α inhalation was significantly higher than that in healthy horses. Our results suggest that 8-epi-PGF2α is not a bronchoconstrictor in healthy horses, and a bronchoconstrictor far less efficient than PGF2α and U46619 at the same dose in heaves-susceptible horses [less ▲]

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See detailDistinct pathways in the over-expression of matrix metalloproteinases in human fibroblasts by relaxation of mechanical tension.
Lambert, Charles ULg; Colige, Alain ULg; Munaut, Carine ULg et al

in Matrix Biology (2001), 20(7), 397-408

The aim of the work was to analyze, on a comparative basis, the signaling pathways operating in the regulation of a panel of matrix metalloproteinases (MMP) expressed by human dermal fibroblasts submitted ... [more ▼]

The aim of the work was to analyze, on a comparative basis, the signaling pathways operating in the regulation of a panel of matrix metalloproteinases (MMP) expressed by human dermal fibroblasts submitted to mechanical stress relaxation by cytochalasin D (CD) and in a retracting collagen gel (RCG). The mRNA steady-state level of MMPs was measured by a quantitative RT-PCR procedure using a synthetic RNA as internal standard. In monolayer, most MMPs were barely detected, except MMP-2. Disruption of the actin stress fibers by CD induced a moderate increase of MMP-2 mRNA and a much larger stimulation of MMP-3, -9, -13 and -14 mRNAs. In RCG, a significant up-regulation of these MMPs was also observed although to a lower extent than in CD-treated monolayers. Among the investigated MMPs, the MMP-8 and -11 were not reproducibly detected. MMP-2 was processed to its active form both by CD and in RCG. The CD-induced up-regulation of gene expression was largely repressed by blocking protein synthesis by cycloheximide for all the MMPs, by inhibiting the tyrosine-kinases of the src family by herbimycin A for all MMPs, except MMP-2, and by inhibiting the TPA-inducible PKC isoforms by bisindoyl maleimide for all MMPs, except MMP-14. The up-regulation induced by stress relaxation in RCG was protein synthesis-dependent for MMP-2 and MMP-13, tyrosine kinases-dependent for MMP-3 and MMP-13, as previously described for MMP-1. Inhibiting TPA-inducible PKC did not affect any MMP in RCG except MMP-13, which was strongly induced. The processing of MMP-2 was tyrosine kinases-dependent but PKC-independent. Inhibitors of the ERK1,2 and p38 MAP kinases pathways diversely affected the MMPs expression. Inhibiting the Rho-kinase activity by Y-27632 was inactive. These results point to the potent regulation operated by the status of the cytoskeleton on the cell phenotype, and to distinct regulatory pathways involved in the control of different MMPs expression. [less ▲]

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See detailLes manuels scolaires en Belgique francophone
Merenne-Schoumaker, Bernadette ULg

in Travaux de l’Institut de Géographie de Reims (2001), (109-110), 83-91

This article tries to evaluate the evolution of both the status and the content of geography’s textbooks in the French part of Belgium. With this aim in view, three periods are analysed : before the ... [more ▼]

This article tries to evaluate the evolution of both the status and the content of geography’s textbooks in the French part of Belgium. With this aim in view, three periods are analysed : before the renovation of the teaching, the years 1980-1990 and today. Through the mutations, narrows links are confirmed between the teaching’s aims and the role devoted to the textbooks. [less ▲]

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See detailThe prevalence of mycotoxins in Kashin-Beck disease
Haubruge, Eric ULg; Chasseur, Camille; Debouck, Catherine et al

in International Orthopaedics (2001), 25

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See detailThe GL bibliography and an interactive database (Poster contribution)
Pospieszalska-Surdej, Anna ULg; Surdej, Jean ULg; Detal, Alain ULg et al

in Brainerd, T. G.; Kochanek, C. S. (Eds.) Proceedings of the Conference "Gravitational lensing: recent progress and future goals" (2001)

It is now possible to directly access, via the Internet, a bibliographical database on Gravitational Lensing (GL) literature. The Interactive Gravitational Lensing Bibliography (IGLB) totalizes more than ... [more ▼]

It is now possible to directly access, via the Internet, a bibliographical database on Gravitational Lensing (GL) literature. The Interactive Gravitational Lensing Bibliography (IGLB) totalizes more than 2400 titles of published articles in scientific journals and meeting proceedings (except those fully dedicated to Gravitational Lenses) as well as papers submitted to the e-Print archive. This database is a product from the Gravitational Lensing Bibliography first presented in 1993 (Proceedings of the 31st Liege International Astrophysical Colloquium). It is easy to do field based searches for title keywords, authors (using boolean operators), year and journal (a pull-down list of the most cited journals is available). Access to the original version of published articles as well as to preprints submitted to the e-Print archive at the URL address http://xxx.lanl.gov/ is also provided. This database is updated approximately every two months. The "complete" bibliography of published articles is also available in the form of Latex and PostScript files. The IGLB can be accessed at the URL: http://vela.astro.ulg.ac.be/grav_lens [less ▲]

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See detailThe relevance of HST observations for studies of quasars, gravitational lenses and intervening gas clouds "
Surdej, Jean ULg; Claeskens, Jean-François ULg; Jean, C. et al

in Space Sciences, Vol. II, Part 1: Space Scientific Research in Belgium (2001)

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See detailIron microbial communities in Belgian Frasnian carbonate mounds
Boulvain, Frédéric ULg; De Ridder, C.; Mamet, B. et al

in Facies (2001), 44

The Belgian Frasnian carbonate mounds occur in three stratigraphic levels in an overall backstepping succession. Petit-Mont and Arche Members form the famous red and grey “marble” exploited for ornamental ... [more ▼]

The Belgian Frasnian carbonate mounds occur in three stratigraphic levels in an overall backstepping succession. Petit-Mont and Arche Members form the famous red and grey “marble” exploited for ornamental stone since Roman times. The evolution and distribution of the facies in the mounds is thought to be associated with ecologic evolution and relative sea-level fluctuations. Iron oxides exist in five forms in the Frasnian mounds; four are undoubtedly endobiotic organized structures: (1) microstromatolites and associated forms (blisters, veils...), possibly organized in “endostromatolites”; (2) hematitic coccoids and (3) non dichotomic filaments. The filaments resemble iron bacteria of the Sphaerotilus- Leptothrix "group"; (4) networks of dichotomic filaments ascribable to fungi; (5) a red ferruginous pigment dispersed in the calcareous matrix whose distribution is related to the mound facies type. The endobiotic forms developed during the edification of the mounds, before cementation by fibrous calcite. The microbial precipitation of iron took place as long as the developing mounds were bathed by water impoverished in oxygen. [less ▲]

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See detailA 4-year study of the mycological aspects of Kashin-Beck disease in Tibet
Chasseur, Camille; Suetens, Carl; Michel, Valérie et al

in International Orthopaedics (2001), 25

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See detailGlutathione S-transferases of Aulacorthum solani and Acyrthosiphon pisum: partial purification and characterization.
Francis, Frédéric ULg; Haubruge, Eric ULg; Gaspar, Charles ULg et al

in Comparative Biochemistry & Physiology Part B (2001), 129(1), 165-71

Glutathione S-transferases (GST) play an important role in the detoxification of many substances including allelochemicals from plants. Brassicaceae plants contain glucosinolates and emit volatile ... [more ▼]

Glutathione S-transferases (GST) play an important role in the detoxification of many substances including allelochemicals from plants. Brassicaceae plants contain glucosinolates and emit volatile isothiocyanates which affect the GST system. A comparison of the GST of two aphid species, the generalist Aulacorthum solani found on Brassicaceae and the Fabaceae specialist Acyrthosiphon pisum, was made to try to explain their respective feeding behaviour. Differences of GST were determined among the two aphid species based on purification by affinity chromatography, SDS-PAGE and on kinetic studies. Purification yields using an epoxy-activated Sepharose 6B column were highly different for the two aphid species (18% and 34% for A. solani and A. pisum, respectively). These variations were confirmed by SDS-PAGE. While only a 27-kDa band was observed for A. pisum, two bands of approximately 25-kDa were visualized for the generalist aphid, A. solani. Considering the kinetic results, differences of Km and Vmax were observed following the aphid species when a range of substrates (CDNB and DCNB) and GSH concentrations were tested. Studies on the detoxification enzymes of generalist and specialist herbivores would be undertaken to determine accurately the effect of the host plant on the organisms eating them, particularly in terms of biochemical and ecological advantages. [less ▲]

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See detailPre-and post-harvest application of antagonistic yeasts for the control of gray and blue mold: efficacy and monitoring
De Clercq, D.; Dickburt, C.; Lepoivre, Philippe ULg et al

in Phytopathology (2001), 91(6), 21

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See detailCompte-rendu de Daniel Giovannangeli, Le retard de la conscience. Husserl, Sartre, Derrida
Leclercq, Bruno ULg

in Recherches Husserliennes (2001), 16

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See detailColomb interactions and the 17 August 1999 Izmit, Turkey earthquake
King, G. C. P.; Hubert, Aurelia ULg; Nalbant, S. S. et al

in Comptes Rendu de l’Académie des Sciences de Paris - Série 2. Sciences de la terre et des planètes (2001), 333(rie 2. Sciences de la terre et des planètes), 557-569

At 00:02 GMT (03:02 local time) on 17 August, 1999 a magnitude 7.4 (Ms) earthquake occurred 100 km east of Istanbul causing extensive destruction. The event was expected and several scientists have ... [more ▼]

At 00:02 GMT (03:02 local time) on 17 August, 1999 a magnitude 7.4 (Ms) earthquake occurred 100 km east of Istanbul causing extensive destruction. The event was expected and several scientists have published and attempted to publicize the danger. A paper on stress interactions for NW Turkey (J. Geophys. Res. 103 (1998) 24466–24469) concluded that “by combining the stress change map with the map of active faulting, likely locations for the occurrence of future earthquakes can be refined; faults in the Izmit Bay area, the western part of Biga Peninsula, the Saroz Gulf and a part of western Sea of Marmara must be regarded as posing a specific hazard”. An extension of that study is described here. It is shown that the Izmit (1999) earthquake loaded faults both to the east and west of the Izmit rupture. About three months after the Izmit event an M 7.2 earthquake occurred with an epicenter at Duzce extending the Izmit rupture to the east. In the Marmara Sea, west of Izmit, faults have been loaded by between 1 and 5 bar; 5 to 30 % of typical earthquake stress drops in the region suggesting the likelihood of a future event. The risk of a major event on a fault depends not just on stress increases associated with an individual earthquake, but also on the longer-term earthquake history and on tectonic loading. The roles of both are examined over two time periods from 1900 to 1999 and 1700 to 1999. Whatever interpretation we place on the data we conclude that one or two events as great or greater than the recent one is likely to occur within the next few decades near to the northern coast of the Marmara Sea [less ▲]

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See detailEthno-agricultural approach to the rural environment in the prevention of Kashin-Beck disease
Malaisse, François ULg; Haubruge, Eric ULg; Mathieu, Françoise et al

in International Orthopaedics (2001), 25

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See detailComparison of inulin with urea as dilutional markers of bronchoalveolar lavage in healthy and heaves-affected horses.
Kirschvink, Nathalie; Fievez, Laurence ULg; Dogne, S. et al

in Veterinary Research (2001), 32(2), 145-154

Solute analysis in bronchoalveolar lavage fluid involves the use of dilutional markers to correct for variable recovery of pulmonary epithelial lining fluid (PELF). Urea is the best characterised ... [more ▼]

Solute analysis in bronchoalveolar lavage fluid involves the use of dilutional markers to correct for variable recovery of pulmonary epithelial lining fluid (PELF). Urea is the best characterised endogenous marker, whereas inulin appears to meet the requirements of an exogenous marker. In horses, the use of inulin has never been investigated and the impact of lower airway diseases such as heaves, on PELF recovery is unknown. In this study, five healthy and five heaves-affected horses underwent airway endoscopy and bronchoalveolar lavage. PELF recovery from bronchoalveolar lavage was calculated by the inulin and the urea method. The inulin method was compared to the urea method and differences between healthy and heaves-affected horses were analysed. From a technical and analytical point of view, inulin fulfilled the requirements of a marker of dilution as well as urea. When both healthy and heaves-affected horses groups were pooled together, PELF recovery calculated by the inulin method was significantly higher than by the urea method (6.43+/-4.08% versus 0.789+/-0.299%, P < 0.005). No significant differences were observed between healthy and heaves-affected horses, neither by the inulin nor by the urea method. Inulin did not present major advantages over urea, but the combined use of both markers can improve the standardisation of studies comparing PELF compounds, by providing upper limits (inulin dilution) and lower limits (urea dilution) of PELF recovery. [less ▲]

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See detailLow TSH requirement and goiter in transgenic mice overexpressing IGF-1 and IGF-1R in the thyroid gland
Clément, S.; Refetoff, S.; Robaye, B. et al

in Endocrinology (2001), 142(12), 5131-5139

Through the cAMP signaling pathway, TSH stimulates thyroid follicular cell proliferation, differentiation, and function. Although the autocrine production of IGF-I in the thyroid gland suggests an ... [more ▼]

Through the cAMP signaling pathway, TSH stimulates thyroid follicular cell proliferation, differentiation, and function. Although the autocrine production of IGF-I in the thyroid gland suggests an important physiological function for this factor in these processes, the exact role of the IGF-I/IGF-I receptor system in vivo remains unclear. Although the mitogenic action of TSH requires the presence of IGF-I or insulin in primary culture of dog and human thyroid cells, IGF-I has an effect equal to and independent of the effect of TSH on cell proliferation in rat thyroid cell lines and may even be the main growth regulator in this case. To investigate the in vivo function of the IGF-I/IGF-I receptor system, transgenic mice overexpressing human IGF-I, IGF-I receptor, or both in the thyroid were generated. Adult transgenic mice did not present external signs of thyroid dysfunction, but mice overexpressing both transgenes had significantly increased gland weight and follicular lumen area. A decreased TSH level together with a slightly increased serum T4 concentration and increased thyroidal iodine uptake were also observed, suggesting that IGF-I and IGF-I receptor stimulate thyroid function to some extent in vivo [less ▲]

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See detailLa résilience hors la loi
Born, Michel ULg; Boët, Sylvie

in Cyrulnik, B. (Ed.) La résilience : le réalisme de l'espérance (2001)

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See detailMonitoring of erythropoiesis by the serum transferrin receptor and erythropoietin.
Fillet, Georges ULg; Beguin, Yves ULg

in Acta Clinica Belgica (2001), 56(3), 146-54

Virtually all cells have transferrin receptors (a transmembrane glycoprotein) on their surface but in a normal adult, 80% of them are in the erythroid marrow. Some of them are lost into the circulation ... [more ▼]

Virtually all cells have transferrin receptors (a transmembrane glycoprotein) on their surface but in a normal adult, 80% of them are in the erythroid marrow. Some of them are lost into the circulation where they can be measured by immuno-assays. A direct and highly significant correlation exists between serum transferrin receptor level and erythron transferrin uptake in humans. The measurement of serum transferrin receptor has wide clinical applications for the quantitation of erythropoiesis. It can be used to study erythropoiesis in situations in which ferrokinetics is not acceptable such as pregnancy. It is particularly useful for serial studies, e.i., for monitoring the recovery of erythropoiesis after stem cell transplantation or after treatment with erythropoietin. Combined with the determination of serum erythropoietin, both evaluated in relation to the degree of anemia, they provide a physiological approach to the diagnosis of anemia. Thus, the simultaneous determination of hematocrit, reticulocytes, serum transferrin receptor and serum erythropoietin has high discriminatory value in distinguishing between a defect in erythroid proliferation, maturation or red cell survival. It is also particularly useful for detecting the presence of multiple mechanisms of anemia in the same patient. [less ▲]

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See detailSpatialité et temporalité du vécu du bien-être
Manfredini, Tiber ULg; Gavray, Claire ULg; Born, Michel ULg

in Bawin, B. (Ed.) L'exclusion et l'insécurité d'existence en milieu urbain (2001)

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