Publications ORBi OA
Bookmark and Share    
Full Text
Peer Reviewed
See detailSoluble forms of VEGF receptor-1 and -2 promote vascular maturation via mural cell recruitment.
LORQUET, Sophie ULiege; Berndt, Sarah; Blacher, Silvia ULiege et al

in FASEB Journal (2010), 24(10), 3782-95

Two soluble forms of vascular endothelial growth factor (VEGF) receptors, sVEGFR-1 and sVEGFR-2, are physiologically released and overproduced in some pathologies. They are known to act as anti-VEGF ... [more ▼]

Two soluble forms of vascular endothelial growth factor (VEGF) receptors, sVEGFR-1 and sVEGFR-2, are physiologically released and overproduced in some pathologies. They are known to act as anti-VEGF agents. Here, we report that these soluble receptors contribute to vessel maturation by mediating a dialogue between endothelial cells (EC) and mural cells that leads to blood vessel stabilization. Through a multidisciplinary approach, we provide evidences that these soluble VEGF receptors promote mural cell migration through a paracrine mechanism involving interplay in EC between VEGF/VEGFR-2 and sphingosine-1- phosphate type-1 (S1P)/S1P1 pathways that leads to endothelial nitric oxyde synthase (eNOS) activation. This new paradigm is supported by the finding that sVEGFR-1 and -2: 1) induce an eNOS-dependent outgrowth of a mural cell network in an ex vivo model of angiogenesis, 2) increase the mural cell coverage of neovessels in vitro and in vivo, 3) promote mural cell migration towards EC, 4) stimulate endothelial S1P1 overproduction and eNOS activation that promote the migration and the recruitment of neighboring mural cells. These findings provide new insights into mechanisms regulating physiological and pathological angiogenesis and vessel stabilization. [less ▲]

Detailed reference viewed: 169 (44 ULiège)
Full Text
Peer Reviewed
See detailReliable hydraulic numerical modeling with multiblock grids and linked models
Erpicum, Sébastien ULiege; Archambeau, Pierre ULiege; Dewals, Benjamin ULiege et al

in Proc. Int. Conf. SimHydro 2010: Hydraulic modeling and uncertainty (2010)

Detailed reference viewed: 20 (4 ULiège)
Full Text
Peer Reviewed
See detailContribution théorique et numérique a la modélisation des écoulements mixtes diphasiques hautement transitoires
Kerger, François ULiege; Archambeau, Pierre ULiege; Dewals, Benjamin ULiege et al

in Proc. Int. Conf. SimHydro 2010: Hydraulic modeling and uncertainty (2010)

Detailed reference viewed: 67 (7 ULiège)
Full Text
Peer Reviewed
See detailSHIP-1 inhibits CD95/APO-1/Fas-induced apoptosis in primary T lymphocytes and T leukemic cells by promoting CD95 glycosylation independently of its phosphatase activity
Charlier, Edith ULiege; Condé, Claude ULiege; Zhang, Jing et al

in Leukemia : Official Journal of the Leukemia Society of America, Leukemia Research Fund, U.K (2010)

SHIP-1 functions as a negative regulator of immune responses by hydrolyzing phosphatidylinositol-3,4,5-triphosphate generated by PI 3-kinase activity. As a result, SHIP-1 deficiency in mice results in ... [more ▼]

SHIP-1 functions as a negative regulator of immune responses by hydrolyzing phosphatidylinositol-3,4,5-triphosphate generated by PI 3-kinase activity. As a result, SHIP-1 deficiency in mice results in myeloproliferation and B cell lymphoma. On the other hand, SHIP-1 deficient mice have a reduced T cell population, but the underlying mechanisms are unknown. In this work, we hypothesized that SHIP-1 plays anti-apoptotic functions in T cells upon stimulation of the death receptor CD95/APO-1/Fas. Using primary T cells from SHIP-1-/- mice and T leukemic cell lines, we report here that SHIP-1 is a potent inhibitor of CD95-induced death. We observed that a small fraction of the SHIP-1 pool is localized to the endoplasmic reticulum where it promotes CD95 glycosylation. This post-translational modification requires an intact SH2 domain of SHIP-1, but is independent of its phosphatase activity. The glycosylated CD95 fails to oligomerize upon stimulation, resulting in impaired DISC formation and downstream apoptotic cascade. These results uncover an unanticipated inhibitory function for SHIP-1 and emphasize the role of glycosylation in the regulation of CD95 signaling in T cells. This work may also provide a new basis for therapeutic strategies using compounds inducing apoptosis through the CD95 pathway on SHIP-1 negative leukemic T cells. [less ▲]

Detailed reference viewed: 110 (16 ULiège)
Full Text
See detailChapter 6 : Agrometeorological forecasting
Gommes, René; Das, Haripada; Mariani, Luigi et al

in WMO/CAgM Guide to Agricultural Meteorological Practices (GAMP) WMO n° 134 (2010)

This chapter presents a set of tools and techniques for crop yield forecasting.

Detailed reference viewed: 73 (5 ULiège)
Full Text
Peer Reviewed
See detailNational Forest Inventories Pathways for Common Reporting: Belgium (Walloon Region)
Rondeux, Jacques ULiege; Sanchez, Christine; Latte, Nicolas ULiege

in Tomppo, Erkki; Gschwantner, Thomas; Lawrence, Mark (Eds.) et al National Forest Inventories. Pathways for Common Reporting (2010)

Detailed reference viewed: 53 (17 ULiège)
Full Text
See detailModave/Modave : elements du Mésolithique recent de la couche 4b-delta du Trou Al'Wesse
Miller, Rebecca ULiege; Otte, Marcel ULiege; Stewart, John

in Chronique de l'Archéologie Wallonne (2010), 17

Detailed reference viewed: 23 (0 ULiège)
Full Text
Peer Reviewed
See detailEffect of exogenous circulating anti-bPL antibodies on bovine placental lactogen measurements in foetal samples.
Alvarez Oxiley, Andrea Vivian ULiege; Melo de Sousa, Noelita ULiege; Hornick, Jean-Luc ULiege et al

in Acta Veterinaria Scandinavica (2010), 52

BACKGROUND: The involvement of placental lactogen (PL) in the regulation of foetal growth has been investigated in different species by in vivo immunomodulation techniques. However, when circulating ... [more ▼]

BACKGROUND: The involvement of placental lactogen (PL) in the regulation of foetal growth has been investigated in different species by in vivo immunomodulation techniques. However, when circulating antibodies are present together with the hormone, the procedure for hormonal measurement becomes considerably complex. The aim of this study was the immunoneutralization of bovine placental lactogen (bPL) concentrations in bovine foetal circulation by direct infusion of rabbit anti-bPL purified immunoglobulins (IgG) via a foetal catheter (in vivo study). The ability of a RIA based on guinea pig anti-bPL antiserum, for the measurement of bPL concentrations in samples containing exogenous rabbit anti-bPL immunoglobulins, was also analyzed in in vitro and in vivo conditions. METHODS: Six bovine foetuses were chronic cannulated on the aorta via the medial tarsal artery. Infusion of rabbit anti-bPL IgG was performed during late gestation. Pooled rabbit anti-bPL antisera had a maximal neutralization capacity of 25 microg bPL/mL of immunoglobulin. Interference of rabbit anti-bPL immunoglobulin with radioimmunoassay measurement using guinea pig anti-bPL as primary antibody was first evaluated in vitro. Polyclonal anti-bPL antibodies raised in rabbit were added in foetal sera to produce 100 samples with known antibodies titers (dilutions ranging from 1:2,500 till 1:1,280,000). RESULT(S): Assessment of the interference of rabbit anti-bPL antibody showed that bPL concentrations were significantly lower (P < 0.05) in samples added with dilutions of rabbit antiserum lower than 1:80,000 (one foetus) or 1:10,000 (four foetuses). It was also shown that the recovery of added bPL (12 ng/mL) was markedly reduced in those samples in which exogenous rabbit anti-bPL were added at dilutions lower than 1:20,000. Concentrations of foetal bPL were determined in samples from cannulated foetuses. In foetuses 1 and 6, bPL concentrations remained almost unchanged (<5 ng/mL) during the whole experimental period. In Foetus 3, bPL concentrations decreased immediately after IgG infusion and thereafter, they increased until parturition. CONCLUSION(S): The use of a bPL RIA using a guinea pig anti-bPL as primary antiserum allowed for the measurement of bPL concentrations in foetal plasma in presence of rabbit anti-bPL IgG into the foetal circulation. Long-term foetal catheterization allowed for the study of the influence of direct infusion of anti-bPL IgG on peripheral bPL concentrations in bovine foetuses. [less ▲]

Detailed reference viewed: 130 (17 ULiège)
Full Text
Peer Reviewed
See detailContractile reserve assessed using dobutamine echocardiography predicts left ventricular reverse remodeling after cardiac resynchronization therapy: prospective validation in patients with left ventricular dyssynchrony.
Sénéchal, Mario; Lancellotti, Patrizio ULiege; Magne, Julien ULiege et al

in Echocardiography (2010), 27(6), 668-76

Background: The presence of viable myocardium may predict response to cardiac resynchronization therapy (CRT). The aim of this study is to evaluate in patients with left ventricular (LV) dyssynchrony ... [more ▼]

Background: The presence of viable myocardium may predict response to cardiac resynchronization therapy (CRT). The aim of this study is to evaluate in patients with left ventricular (LV) dyssynchrony whether response to CRT is related to myocardial viability in the region of the pacing lead. Methods: Forty-nine consecutive patients with advanced heart failure, LV ejection fraction < 35%, QRS duration > 120 ms and intraventricular asynchronism ≥ 50 ms were included. Dobutamine stress echocardiography was performed within the week before CRT implantation. Resting echocardiography was performed 6 months after CRT implantation. Viability in the region of LV pacing lead was defined as the presence of viability in two contiguous segments. Response to CRT was defined by evidence of reverse LV remodeling (≥15% reduction in LV end-systolic volume). Results: Thirty-one patients (63%) were identified as responders at follow-up. The average of viable segments was 5.9 ± 2 in responders and 3.2 ± 3 in nonresponders (P = 0.0003). Viability in the region of the pacing lead had a sensitivity of 94%, a specificity of 67%, a positive predictive value of 83%, and a negative predictive value of 86% for the prediction of response to CRT. Conclusions: In patients with LV dyssynchrony, reverse remodeling after CRT requires viability in the region of the pacing lead. This simple method using echocardiography dobutamine for the evaluation of local viability (i.e., viability in two contiguous segments) may be useful to the clinician in choosing the best LV lead positioning. [less ▲]

Detailed reference viewed: 37 (2 ULiège)
Full Text
Peer Reviewed
See detailHydroCube: a new entity-relationship hydrogeological data model
Wojda, Piotr; Brouyère, Serge ULiege; Derouane, Johan et al

in Hydrogeology Journal (2010), 18(8), 1953-1962

Managing, handling, and accessing hydrogeological information depend mainly on the applied hydrogeological data models, which differ between institutions and across countries. Growing interest in ... [more ▼]

Managing, handling, and accessing hydrogeological information depend mainly on the applied hydrogeological data models, which differ between institutions and across countries. Growing interest in hydrogeological information diffusion, combined with a need for information availability, require the convergence of hydrogeological data models to make hydrogeological information accessible to multiple users such as universities, administrations, water suppliers, and research organisations. Furthermore, because hydrogeological studies are complex, they require a large variety of high-quality hydrogeological data with appropriate metadata in clearly designed and coherent structures. A need therefore exist to develop and implement hydrogeological data models that cover, as much as possible, the full hydrogeological domain. To respond to these requirements, a new data model, called HydroCube, has been developed for the Walloon Region in Belgium. The HydroCube model presents an innovative holistic “project-based” approach, which covers a full set of hydrogeological concepts and features, allowing for effective hydrogeological project management. This approach enables to store data about the project localisation, hydrogeological equipment, related observations and measurements. In particular, the model focuses on specialized hydrogeological field experiments, such as pumping and tracer tests. This logical data model uses entity-relationship diagrams and it has been implemented in the MS Access environment as the HydroCube database. It has been additionally enriched with a fully functional user-interface. [less ▲]

Detailed reference viewed: 120 (25 ULiège)
Full Text
Peer Reviewed
See detailNew Biological Investigations on 3-Bromophenyl 6-Acetoxymethyl-2-oxo-2H-1-Benzopyran-3-Carboxylate as Anti-angiogenic Agent
Hemmer, M.; Kempen, Isabelle; De Tullio, Pascal ULiege et al

in Drug Development Research (2010), 71

The development of blood vessels inside tumors is required to provide the nutrients and oxygen needed for tumor growth and to allow the spread of cancer cells at a distance to form metastasis ... [more ▼]

The development of blood vessels inside tumors is required to provide the nutrients and oxygen needed for tumor growth and to allow the spread of cancer cells at a distance to form metastasis. Angiogenesis is also implicated in ocular diseases like age-related macular degeneration. The present work describes the potential anti-angiogenic properties of a coumarinic derivative, 3-bromophenyl 6-acetoxymethyl-2-oxo-2H-1-benzopyran-3-carboxylate (IK9), previously described as a potent inhibitor of HT 1080 fibrosarcoma cell invasion in vitro and tumor growth in vivo. In vivo, ex vivo, and in vitro models were used to delineate the anti-angiogenic properties of IK9. The anti-angiogenic effect of IK9 was demonstrated in vivo in a choroidal neovascularization mice model and additionally ex vivo in a rat aortic ring assay where it was more active than the known matrix metalloproteinase inhibitor Ro 28-2653. IK9 did not affect apoptosis, proliferation, or endothelial cell invasiveness in vitro. These findings suggest a complex mechanism of action of the compound via direct or indirect effects on endothelial cell properties. This study identifies IK9 as a new potent inhibitor of angiogenesis and suggests its potential use as a therapeutic agent. [less ▲]

Detailed reference viewed: 93 (15 ULiège)
Full Text
Peer Reviewed
See detailLe tabac et ses effets sur le système endocrinien
VALDES SOCIN, Hernan Gonzalo ULiege; VROONEN, Laurent ULiege; Latta, A. et al

in Revue Médicale de Liège (2010), 65(9), 498-501

Detailed reference viewed: 89 (6 ULiège)
Full Text
Peer Reviewed
See detailEffects of HPV-16 E5, E6 and E7 Proteins on Survival, Adhesion, Migration and Invasion of Trophoblastic Cells
Boulenouar, S.; Weyn, C.; Van Noppen, M. et al

in Carcinogenesis (2010), 31(3), 473-80

Amongst high-risk human papillomaviruses (HPV), HPV-16 infection is the most prevalent causative factor for cervical cancer. Beside other mucosal targets, HPV-16 was reported to infect the placenta and to ... [more ▼]

Amongst high-risk human papillomaviruses (HPV), HPV-16 infection is the most prevalent causative factor for cervical cancer. Beside other mucosal targets, HPV-16 was reported to infect the placenta and to replicate in trophoblastic cells. Since these cells share invasive properties of tumoral cells, they represent an ideal model to investigate several oncogenic processes. In the present work, we analyzed the impacts of HPV-16 E5, E6 and E7 oncoproteins on the trophoblastic model. Our results showed that E5 impaired the viability of trophoblastic and cervical cell lines but E6 and E7, favouring cell growth, neutralised the E5 cytotoxic effect. In addition, E5 decreased the adhesiveness of trophoblastic cells to the tissue culture plastic and to endometrial cells similarly as previously described for E6 and E7. E5 and E6 plus E7 increased also their migration and their invasive properties. Cells expressing HPV-16 early proteins under the control of the LCR endogenous promoter displayed growth advantage and were also more motile and invasive compared to control cells. Interestingly, the E-cadherin was down regulated in trophoblastic cells expressing E5, E6 and E7. NF-kB and AP-1 activities were also enhanced. In conclusion, HPV-16 early proteins enhanced trophoblastic growth and intensify the malignant phenotype by impairing cell adhesion leading to increased cellular motile and invasive properties. HPV-16 E5 participated, with E6 and E7, in these changes by impairing Ecadherin expression, a hallmark of malignant progression. [less ▲]

Detailed reference viewed: 117 (6 ULiège)
Full Text
Peer Reviewed
See detailInsight on variables leading to burnout in cancer physicians
Bragard, Isabelle ULiege; Libert, Y.; Etienne, Anne-Marie ULiege et al

in Journal of Cancer Education : The Official Journal of the American Association for Cancer Education (2010), 25

Detailed reference viewed: 43 (5 ULiège)
Full Text
Peer Reviewed
See detailApplication of multiple-point geostatistics on modelling groundwater flow and transport in a cross-bedded aquifer
Huysmans, Marijke; Dassargues, Alain ULiege

in Atkinson, P. M. M.; Lloyd, C. D. D. (Eds.) GeoENV VII – Geostatistics for Environmental Applications, Quantitative Geology and Geostatistics (2010)

In this work, the problem of modelling groundwater flow and transport in a heterogeneous environment with complex geological structures is addressed. This study demonstrates how a training image can be ... [more ▼]

In this work, the problem of modelling groundwater flow and transport in a heterogeneous environment with complex geological structures is addressed. This study demonstrates how a training image can be constructed based on geological and hydrogeological field data and how multiple-point geostatistics can be applied to determine the impact of complex geological heterogeneity on groundwater flow and transport in a real aquifer. [less ▲]

Detailed reference viewed: 63 (12 ULiège)
Full Text
See detailLe bananier: comment obtenir du matériel de plantation indemne de virus
De Clerck, Caroline ULiege; Lassois, Ludivine ULiege

Diverse speeche and writing (2010)

Detailed reference viewed: 73 (11 ULiège)
Full Text
See detailL'assainissement viral des bananiers: une étape primordiale dans la préservation de la biodiveristé
Lassois, Ludivine ULiege; Castaigne, Michel; D'haes, François

Diverse speeche and writing (2010)

Le laboratoire de Phytopathologie de Gembloux Agro-Bio Tech a développé les techniques permettant la détection et l’assainissement des plantules de bananiers virosés. Ce laboratoire travaille en étroite ... [more ▼]

Le laboratoire de Phytopathologie de Gembloux Agro-Bio Tech a développé les techniques permettant la détection et l’assainissement des plantules de bananiers virosés. Ce laboratoire travaille en étroite collaboration avec Bioversity International afin de fournir au Centre de Transit International, et à fortiori à la communauté internationale, des bananiers indemnes de virus. Les bananiers ainsi obtenus peuvent donc être diffusés largement sans craintes d’une épidémie quelconque. Ce travail d’assainissement contribue non seulement à la sauvegarde de la biodiversité mais également à sa valorisation en rendant saines des variétés qui étaient virosées et non exploitables. Jusqu’à présent l’ITC a fourni 15 000 plantules saines de bananiers dans 335 régions réparties dans 100 pays. Ce travail conjoint de conservation et d’assainissement permet de valoriser la grande diversité génétique du bananier au service de la communauté internationale. [less ▲]

Detailed reference viewed: 100 (9 ULiège)
Full Text
Peer Reviewed
See detailRole of delta-like-4/Notch in the formation and wiring of the lymphatic network in zebrafish.
Geudens, I.; Herpers, R.; Hermans, K. et al

in Arteriosclerosis, Thrombosis, and Vascular Biology (2010), 30(9), 1695-702

OBJECTIVE: To study whether Notch signaling, which regulates cell fate decisions and vessel morphogenesis, controls lymphatic development. METHODS AND RESULTS: In zebrafish embryos, sprouts from the axial ... [more ▼]

OBJECTIVE: To study whether Notch signaling, which regulates cell fate decisions and vessel morphogenesis, controls lymphatic development. METHODS AND RESULTS: In zebrafish embryos, sprouts from the axial vein have lymphangiogenic potential because they give rise to the first lymphatics. Knockdown of delta-like-4 (Dll4) or its receptors Notch-1b or Notch-6 in zebrafish impaired lymphangiogenesis. Dll4/Notch silencing reduced the number of sprouts producing the string of parchordal lymphangioblasts; instead, sprouts connecting to the intersomitic vessels were formed. At a later phase, Notch silencing impaired navigation of lymphatic intersomitic vessels along their arterial templates. CONCLUSIONS: These studies imply critical roles for Notch signaling in the formation and wiring of the lymphatic network. [less ▲]

Detailed reference viewed: 19 (3 ULiège)
Full Text
Peer Reviewed
See detailHistological and transcriptional study of angiogenesis and lymphangiogenesis in uninvolved skin, acute pinpoint lesions and established psoriasis plaques: an approach of vascular development chronology in psoriasis
Henno, Audrey ULiege; Blacher, Silvia ULiege; Lambert, Charles ULiege et al

in Journal of Dermatological Science (2010), 57(3), 162-169

Background Dysregulation of angiogenesis and lymphangiogenesis could participate in psoriasis pathogenesis. Analysis of nascent psoriasis lesions should help at identifying early vascular anomalies ... [more ▼]

Background Dysregulation of angiogenesis and lymphangiogenesis could participate in psoriasis pathogenesis. Analysis of nascent psoriasis lesions should help at identifying early vascular anomalies. Objective To analyse vascular development, angiogenesis and lymphangiogenesis markers expression in uninvolved skin in psoriatic patients (N), early psoriasis lesions or pinpoints (PP) and psoriasis plaques (PSO). Methods Skin biopsies were taken in 17 patients in N and in PSO and/or PP. The mRNA steady-state level of angiogenesis and lymphangiogenesis markers was measured by RT-PCR. Immunohistochemistry was performed for von Willebrand factor, podoplanin, Ki-67 and VEGFR3. Blood (BV) and lymphatic (LV) vessels expansion was measured by computer-assisted morphometry. Results Clinical and epidermal aspects indicated that PP are intermediate between N and PSO. While total BV area was already increased in PP similarly to PSO as compared to N, LV area in PP was intermediate between N and PSO. Mean LV size was identical in N and PP and increased in PSO, mean BV size in PP being intermediate between N and PSO. VEGF-A 189 variant was increased in PP as compared to N and PSO. As compared to N, angiogenesis markers (VEGF-A isoforms, PlGF, VEGFR2, NRP-1), VEGF-C and NRP-2 were similarly increased in PP and PSO. Keratin 16 and the lymphangiogenesis markers (VEGFR3, prox-1) were intermediate in PP. Conclusion These data suggest that the expansion of lymphatic vessels occurs after blood vascular development in psoriasis. Expansion of BV in PP could be followed by vessel enlargement during progression to PSO, in parallel with a decreased VEGF-A 189/VEGF-A 121 balance in plaques [less ▲]

Detailed reference viewed: 80 (11 ULiège)
Full Text
Peer Reviewed
See detailTIMP-2 binding with cellular MT1-MMP stimulates invasion-promoting MEK/ERK signaling in cancer cells
Sounni, Nor Eddine ULiege; Rozanov, D. V.; Remacle, A. G. et al

in International Journal of Cancer = Journal International du Cancer (2010), 126(5), 1067-78

Both invasion-promoting MT1-MMP and its physiological inhibitorTIMP-2 play a significant role in tumorigenesis and are identified in the most aggressive cancers. Despite its antiproteolytic effects in ... [more ▼]

Both invasion-promoting MT1-MMP and its physiological inhibitorTIMP-2 play a significant role in tumorigenesis and are identified in the most aggressive cancers. Despite its antiproteolytic effects in vitro, clinical data suggest that TIMP-2 expression is positively associated with tumor recurrence, thus emphasizing the wide-ranging role of TIMP-2 in malignancies. To shed light on this role of TIMP-2, we report that low concentrations of TIMP-2, by interacting with MT1-MMP (a specific membrane receptor of TIMP-2), induce the MEK/ERK signaling cascade in fibrosarcoma HT1080 cells which express MT1-MMP naturally. TIMP-2 binding with cell surface-associated MT1-MMP stimulates phosphorylation of MEK1/2, which is upstream of ERK1/2, and the ERK1/2 substrate p90RSK. Consistent with volumes of literature, we confirmed that the activation of ERK stimulated cell migration. Both the transcriptional silencing of MT1-MMP and the inhibition of MEK1/2 reversed the signaling effects of TIMP-2/MT1-MMP while the active site-targeting MMP inhibitor GM6001 did not. Our data suggest that both the interactions of TIMP-2 with MT1-MMP, which activate the pro-migratory ERK signaling cascade, and the conventional inhibition of MT1-MMP's catalytic activity by TIMP-2, play a role in the invasion-promoting function of MT1-MMP. The TIMP-2-induced stimulation of ERK signaling in cancer cells explains the direct, as opposed to the inverse, association of TIMP-2 expression with poor prognosis in cancer. [less ▲]

Detailed reference viewed: 75 (9 ULiège)