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See detailAcidic extracellular pH induces matrix metalloproteinase-9 expression in mouse metastatic melanoma cells through the phospholipase D-mitogen-activated protein kinase signaling
Kato, Y.; Lambert, Charles ULg; Colige, Alain ULg et al

in Journal of Biological Chemistry (2005), 280(12), 10938-10944

The extracellular pH (pHe) of tumor tissues is often acidic, which can induce the expression of several proteins. We previously showed that production of matrix metalloproteinase-9 (MMP-9) was induced by ... [more ▼]

The extracellular pH (pHe) of tumor tissues is often acidic, which can induce the expression of several proteins. We previously showed that production of matrix metalloproteinase-9 (MMP-9) was induced by culturing cells at acidic pHe (5.4-6.5). Here we have investigated the signal transduction pathway by which acidic pHe induces MMP-9 expression. We found that acidic pHe (5.9) activated phospholipase D (PLD), and inhibition of PLD activity by 1-butanol and Myr-ARF6 suppressed the acidic pHe-induced MMP-9 expression. Exogenous PLD, but not phosphatidylinositol-specific PLC or PLA(2), mimicked MMP-9 induction by acidic pHe. Western blot analysis revealed that acidic pHe increased the steady-state levels of phosphorylated extracellular signal-regulated kinases 1/2 and p38 and that the PLD inhibitors suppressed these increases. Using 5'-deletion mutant constructs of the MMP-9 promoter, we found that the acidic pHe-responsive region was located at nucleotide -670 to -531, a region containing the NF kappa B binding site. A mutation into the NF kappa B binding site reduced, but not completely, the acidic pHe- induced MMP-9 promoter activity, pand NF kappa B activity was induced by acidic pHe. Pharmacological inhibitors specific for mitogen-activated protein kinase kinase 1/2 (PD098059) and p38 (SB203580) attenuated the acidic pHe- induced NF kappa B activity and MMP-9 expression. These data suggest that PLD, mitogen-activated protein kinases (extracellular signal-regulated kinases 1/2 and p38), and NF kappa B mediate the acidic pHe signaling to induce MMP-9 expression. A transcription factor(s) other than NF kappa B may also be involved in the MMP-9 expression. [less ▲]

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See detailInterférométrie destructive: du sol à l’espace
Absil, Olivier ULg

Scientific conference (2005, March 22)

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See detailGraft-versus-tumor effects after allogeneic hematopoietic cell transplantation with nonmyeloablative conditioning
Baron, Frédéric ULg; Maris, M. B.; Sandmaier, B. M. et al

in Journal of Clinical Oncology (2005), 23(9), 1993-2003

Purpose We have used a nonmyelorablative conditioning regimen consisting of total-body irradiation (2 Gy) with or without fludarabine (30 mg/m(2)/d for 3 days) for related and unrelated hematopoietic cell ... [more ▼]

Purpose We have used a nonmyelorablative conditioning regimen consisting of total-body irradiation (2 Gy) with or without fludarabine (30 mg/m(2)/d for 3 days) for related and unrelated hematopoietic cell transplantation (HCT) in patients with hematologic malignancies who were not candidates for conventional HCT because of age, medical comorbidities, or preceding high-dose HCT. This approach relied on graft-versus-tumor (GVT) effects for control of malignancy. Patients and Methods We analyzed GVT effects in 322 patients given grafts from HILA-matched related (n = 192) or unrelated donors (n = 130). Results Of the 221 patients with measurable disease at HCT, 126 (57%) achieved complete (n = 98) or partial (n = 28) remissions. In multivariate analysis, there was a higher probability trend of achieving complete remissions in patients with chronic extensive graft-versus-host disease (GVHD, P = .07). One hundred eight patients (34%) relapsed or progressed. In multivariate analysis, achievement of full donor chimerism was associated with a decreased risk of relapse or progression (P = .002). Grade 2 to 4 acute GVHD had no significant impact on the risk of relapse or progression but was associated with increased risk of nonrelapse mortality and decreased probability of progression-free survival (PFS). Conversely, extensive chronic GVHD was associated with decreased risk of relapse or progression (P = .006) and increased probability of PFS (P = .003). Conclusion New approaches aimed at reducing the incidence of grade 2 to 4 acute GVHD might improve survival after allogeneic HCT after nonmyeloablative conditioning. (c) 2005 by American Society of Clinical Oncology. [less ▲]

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See detailNaître pour disparaître
Van Caillie, Didier ULg

Article for general public (2005)

This vulgarisation paper highlights why so many young firms are almost immediately conducted to business failure. It insists on the problem of under-capitalisation as a key indicator of business failure ... [more ▼]

This vulgarisation paper highlights why so many young firms are almost immediately conducted to business failure. It insists on the problem of under-capitalisation as a key indicator of business failure amongst young firms. [less ▲]

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See detailSa passion : la liberté
Cormann, Grégory ULg

Article for general public (2005)

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See detailInfluence of the nature of the electrolyte on the chiral separation of basic compounds in nonaqueous capillary electrophoresis using heptakis(2,3-di-O-methyl-6-O-sulfo)-beta-cyclodextrin
Servais, Anne-Catherine ULg; Fillet, Marianne ULg; Chiap, Patrice ULg et al

in Journal of Chromatography. A (2005), 1068(1), 143-150

The influence on the enantiomeric resolution of the nature of the cationic BGE component (sodium, ammonium or potassium) and that of the anionic component (chloride, formate, methanesulfonate or ... [more ▼]

The influence on the enantiomeric resolution of the nature of the cationic BGE component (sodium, ammonium or potassium) and that of the anionic component (chloride, formate, methanesulfonate or camphorsulfonate) as well as the concentration of heptakis(2,3-di-O-methyl-6-O-sulfo)-beta-cyclodextrin (HDMS-beta-CD), the selected chiral selector, was studied in nonaqueous capillary electrophoresis (NACE). For this purpose, two D-optimal designs with 33 and 26 experimental points were applied. Three beta-blockers (atenolol, celiprolol and propranolol) and three local anesthetics (bupivacaine, mepivacaine and prilocaine) were selected as basic model compounds. Both cationic and anionic BGE components were found to have a deep impact on the enantiomeric resolution of the investigated analytes but it is the cationic component that has shown the strongest influence. Indeed, in some cases, the change of the latter led to a complete loss of enantioresolution. Based on the observed results, two NACE systems were recommended, namely ammonium formate and potassium camphorsulfonate in a methanolic solution containing HDMS-beta-CD and acidified with formic acid, in order to separate efficiently the enantiomers of basic drugs. (c) 2004 Elsevier B.V. All rights reserved. [less ▲]

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See detailThe massive runaway stars HD 14633 and HD 15137
Boyajian, T. S.; Beaulieu, T. D.; Gies, D. R. et al

in Astrophysical Journal (2005), 621(2, Part 1), 978-984

We present results from a radial velocity study of two runaway O-type stars, HD14633 (ON8.5V) and HD 15137 [O9.5 III(n)]. We find that HD 14633 is a single-lined spectroscopic binary with an orbital ... [more ▼]

We present results from a radial velocity study of two runaway O-type stars, HD14633 (ON8.5V) and HD 15137 [O9.5 III(n)]. We find that HD 14633 is a single-lined spectroscopic binary with an orbital period of 15.4083 days. The second target, HD 15137, is a radial velocity variable and a possible single-lined spectroscopic binary with a period close to 1 month. Both binaries have large eccentricity, small semiamplitude, and a small mass function. We show the trajectories of the stars in the sky based on an integration of motion in the Galactic potential, and we suggest that both stars were ejected from the vicinity of the open cluster NGC 654 in the Perseus spiral arm. The binary orbital parameters and runaway velocities are consistent with the idea that both these stars were ejected by supernova explosions in binaries and that they host neutron star companions. We find that the time of flight since ejection is longer than the predicted evolutionary timescales for the stars. This discrepancy may indicate that the stars have a lower mass than normally associated with their spectral classifications, that they were rejuvenated by mass transfer prior to the supernova, or that their lives have been extended through rapid rotation. [less ▲]

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See detailAutomated method for the determination of a new matrix metalloproteinase inhibitor in ovine plasma and serum by coupling of restricted access material for on-line sample clean-up to liquid chromatography
Chiap, Patrice ULg; Piette, Marie ULg; Evrard, Brigitte ULg et al

in Journal of Chromatography. B : Analytical Technologies in the Biomedical & Life Sciences (2005), 817(1), 109-117

A fully automated liquid chromatographic method was developed for the determination of Ro 28-2653, a new synthetic inhibitor of matrix metalloproteinases (MMPs), in ovine serum and plasma. The method was ... [more ▼]

A fully automated liquid chromatographic method was developed for the determination of Ro 28-2653, a new synthetic inhibitor of matrix metalloproteinases (MMPs), in ovine serum and plasma. The method was based on the coupling of a pre-column packed with restricted access material, namely LiChrospher RP-8 ADS (alkyl diol silica), for sample clean-up to an analytical column containing octyl silica stationary phase. One hundred mul of biological sample, to which 2-propanol was automatically added, were injected onto the ADS pre-column, which was then washed with a washing liquid consisting of a mixture of 25 mM phosphate buffer (pH 7.0) and acetonitrile (90: 10; v/v) for 10 min. By rotation of the switching valve, the analyte was then eluted in the back-flush mode with the LC mobile phase composed of a mixture of acetonitrile and 25 mM phosphate buffer (pH 7.0) (57:43; v/v). The UV detection was performed at 395 nm. The main parameters likely to influence the sample preparation technique were investigated. The method was then validated over a concentration range from 17.5 to 1950 ng/mI, the first concentration level corresponding to the lower limit of quantitation. At this concentration level, the mean bias and the R.S.D. value for intermediate precision were -2.4% and 4.2%, respectively. (C) 2004 Elsevier B.V. All rights reserved. [less ▲]

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See detailLow daunomycin concentrations protect colorectal cancer cells from hypoxia-induced apoptosis
Lechanteur, Chantal ULg; Jacobs, Nathalie ULg; Greimers, Roland ULg et al

in Oncogene (2005), 24(10), 1788-1793

Hypoxia, a common feature of solid tumors, is a direct stress that triggers apoptosis in many cell types. Poor or irregular tumor vascularization also leads to a decreased drug diffusion and cancer cells ... [more ▼]

Hypoxia, a common feature of solid tumors, is a direct stress that triggers apoptosis in many cell types. Poor or irregular tumor vascularization also leads to a decreased drug diffusion and cancer cells distant from blood vessels (hypoxic cells) are exposed to low drug concentrations. In this report, we show that low daunomycin concentrations protect HCT116 colorectal cancer cells from hypoxia-induced apoptosis. While hypoxia induced p53 accumulation without expression of its responsive genes (bax and p21), daunomycin treatment restored p53 transactivation activity and cell cycle progression. We also demonstrated a role for Akt activation in daunomycin-induced protection through phosphorylation and inactivation of the Bcl-2 family proapoptotic factor Bad. Our data therefore suggest that chemotherapy could possibly, because of low concentrations in poorly vascularized tumors, protect cancer cells from hypoxia-induced cytotoxicity. [less ▲]

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See detailIsotopic abundance of nitrogen and carbon in distant comets
Manfroid, Jean ULg; Jehin, Emmanuel ULg; Hutsemekers, Damien ULg et al

in Astronomy and Astrophysics (2005), 432(Letters), 5-8

The [SUP]12[/SUP]C[SUP]14[/SUP]N/[SUP]12[/SUP]C[SUP]15[/SUP]N and [SUP]12[/SUP]C[SUP]14[/SUP]N/[SUP]13[/SUP]C[SUP]14[/SUP]N isotopic ratios have been determined in comets C/1995 O1 (Hale-Bopp), C/2001 Q4 ... [more ▼]

The [SUP]12[/SUP]C[SUP]14[/SUP]N/[SUP]12[/SUP]C[SUP]15[/SUP]N and [SUP]12[/SUP]C[SUP]14[/SUP]N/[SUP]13[/SUP]C[SUP]14[/SUP]N isotopic ratios have been determined in comets C/1995 O1 (Hale-Bopp), C/2001 Q4 (NEAT) and C/2003 K4 (LINEAR) at heliocentric distances of, respectively, 2.7, 3.7 and 2.6 AU. These ratios have also been measured at rË 1 AU. No significant differences were found between all determinations, nor with the value obtained for other comets. If confirmed, the discrepancy between the nitrogen isotopic ratios from optical and millimeter measurements on CN and HCN would rule out HCN as a major parent of the cometary CN radicals. Based on observations collected at the European Southern Observatory, Paranal, Chile (ESO Programmes 270.C-5043, 073.C-0525 and 274.C-5015). [less ▲]

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See detailA study of bright southern slowly pulsating B stars. III. Mode identification for singly-periodic targets in spectroscopy
De Cat, P.; Briquet, Maryline ULg; Daszynska-Daszkiewicz, J. et al

in Astronomy and Astrophysics (2005), 432

We present the results of the mode identification for a sample of 7 bright southern slowly pulsating B stars showing one pulsation frequency in the lambdalambda 413 nm Si II profiles. We combined the ... [more ▼]

We present the results of the mode identification for a sample of 7 bright southern slowly pulsating B stars showing one pulsation frequency in the lambdalambda 413 nm Si II profiles. We combined the results from (1) the method of photometric amplitudes; (2) the moment method; and (3) the amplitude and phase variation across the profile to search for the l and m values of the modes best fitting the data. It is the first time that the applicability of these techniques is tested to a sample of main-sequence g-mode pulsators. Combining the moment method with the amplitude and phase variations across the observed line profile gives an improvement in spectroscopic identification of low degree l g-mode pulsations. Using the variations of the higher order even moments < v[SUP]4[/SUP]> and < v[SUP]6[/SUP]> of the moment method solutions can also help. For HD 181558, HD 24587, HD 140873 and HD 177863, the photometric and spectroscopic results are compatible and point towards (l,m) = (1, +1) sectoral modes. For HD 215573, HD 53921 and HD 92287, the results are inconclusive. Our proposed methodology for mode identification is also applicable to gamma Doradus stars. Based on observations collected with the CAT Telescope of the European Southern Observatory and with the Swiss Photometric Telescope of the Geneva Observatory, both situated at La Silla in Chile. [less ▲]

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See detailL'astronomie dans le monde
Manfroid, Jean ULg

Article for general public (2005)

Trou noir glouton, Matière noire, Integral et le centre de notre Galaxie, Essaim de trous noirs, Carrousel autour de trous noirs, Erosion de trous noirs, Un quasar dans NGC 7319?, Distance de T Tauri ... [more ▼]

Trou noir glouton, Matière noire, Integral et le centre de notre Galaxie, Essaim de trous noirs, Carrousel autour de trous noirs, Erosion de trous noirs, Un quasar dans NGC 7319?, Distance de T Tauri, Chaînon manquant, Des planètes autour d'une naine brune?, Titan, Une quatrième planète pour le pulsar PSR B1257+12, Smart 1, Aurores de Saturne [less ▲]

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See detailDisentangling component spectra of κ Scorpii, a spectroscopic binary with a pulsating primary. II. Interpretation of the line-profile variability
Uytterhoeven, K.; Briquet, Maryline ULg; Aerts, C. et al

in Astronomy and Astrophysics (2005), 432

We analyse the complex short-term SiIII line-profile variability of the spectroscopic binary β Cep star κ Scorpii after orbit subtraction, before and after spectral disentangling. We refine the known ... [more ▼]

We analyse the complex short-term SiIII line-profile variability of the spectroscopic binary β Cep star κ Scorpii after orbit subtraction, before and after spectral disentangling. We refine the known oscillation frequency of the star: f[SUB]1[/SUB]=4.99922 c d[SUP]-1[/SUP] and detect 2f[SUB]1[/SUB]. Variability is also found at frequencies near f[SUB]2[/SUB]≃ 4.85 c d[SUP]-1[/SUP] and f[SUB]3[/SUB]≃ 5.69 c d[SUP]-1[/SUP] or their aliases. These frequencies are not significant if we consider the spectra alone, but they survive our selection after the consideration that they were derived previously from independent ground-based and space photometry by different teams. Moreover, we find dominant variability in the equivalent width with a frequency in the interval [0.22,0.30] c d[SUP]-1[/SUP] which we interpret as the rotational frequency f[SUB]rot[/SUB] of the star. The complex window function does not allow us to determine definite values for f[SUB]2[/SUB], f[SUB]3[/SUB], f[SUB]rot[/SUB]. The variability with f[SUB]1[/SUB] is interpreted as a prograde non-radial oscillation mode with spherical wavenumbers (ℓ,m)=(2,-1) or (1,-1). The additional frequencies are explained in terms of rotational modulation superposed to the main oscillation. We also point out that we cannot disprove the variability in κ Scorpii to originate from co-rotating structures. KOREL disentangling preserves the large-amplitude line-profile variability but its performance for complex low-amplitude variability remains to be studied in detail. Based on observations obtained with the Coudé Échelle Spectrograph on the ESO CAT telescope and with the CORALIE échelle spectrograph on the 1.2-m Euler Swiss telescope, both situated at La Silla, Chile. [less ▲]

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See detailMesure des odeurs dans l'environnement: Synthèse des méthodes et conditions d'usage
Nicolas, Jacques ULg; Otte, Benoît

Report (2005)

In the frame of the project "plan of air in Wallonia", aiming at inventory regulations, standards and measurement methods of air pollutants, this particular report concerns more particularly the ... [more ▼]

In the frame of the project "plan of air in Wallonia", aiming at inventory regulations, standards and measurement methods of air pollutants, this particular report concerns more particularly the measurement of environmental odours. The conditions of the use of each method is discussed. [less ▲]

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See detailLes odeurs dans l'environnement: Sources d'odeur en Région Wallonne
Nicolas, Jacques ULg; Otte, Benoît

Report (2005)

The report was written in the frame of the project "Plan of air in Wallonia" aiming at inventory the policies, standards and measurement methods relative to air quality. The present report concerns more ... [more ▼]

The report was written in the frame of the project "Plan of air in Wallonia" aiming at inventory the policies, standards and measurement methods relative to air quality. The present report concerns more particularly the identification of possible sources of odour in Wallonia. Both the industrial and the agricultural sectors are investigated. [less ▲]

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See detailLes odeurs dans l'environnement: Résumé des législations européennes et nord-américaines
Nicolas, Jacques ULg; Otte, Benoît

Report (2005)

This short report synthesizes the policies concerning odour emissions applicable in Europe and North America. It was written in the frame of the project "Plan of air in Wallonia" aiming at inventory the ... [more ▼]

This short report synthesizes the policies concerning odour emissions applicable in Europe and North America. It was written in the frame of the project "Plan of air in Wallonia" aiming at inventory the policies, standards and measurement methods relative to air quality. [less ▲]

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See detailHutte ou terrier ?
Libois, Roland ULg

Learning material (2005)

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See detailCroque noisettes ou rat d'or ?
Libois, Roland ULg

Learning material (2005)

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