Publications ORBi OA
La formation documentaire à l'UD FAPSE (ULg) : Une tentative de partenariat et d'intégration dans le cursus
in Pochet, Bernard; Thirion, Paul (Eds.) La bibliothèque, partenaire du projet pédagogique : Compte-rendu des ateliers francophones sur la formation documentaire, Gembloux 13-15 octobre 1997 (1998)
Présentation d'une expérience de mise en place d'un cours de formation documentaire à destination d'étudiants de 4ème année universitaire en psychologie et sciences de l'éducation. Intégration de contenus ... [more ▼]
Présentation d'une expérience de mise en place d'un cours de formation documentaire à destination d'étudiants de 4ème année universitaire en psychologie et sciences de l'éducation. Intégration de contenus techniques et méthodologiques, lien avec un besoin individuel réel, partenariat avec les enseignants et intégration au cursus. L'impact de ces formations sur les résultats scolaires des étudiants a également été mesuré. [less ▲]Detailed reference viewed: 161 (53 ULg)
Isolation Of Highly Performant Sulfate Reducers From Sulfate-Rich Environments
Hiligsmann, Serge ; ; Thonart, Philippe
in Biodegradation (1998), 9(3-4), 285-292
Eleven pure strains of sulfate-reducing bacteria have been isolated from lab-scale bioreactors or disposal sites, all featuring relatively high concentrations of sulfate, and from natural environments in ... [more ▼]
Eleven pure strains of sulfate-reducing bacteria have been isolated from lab-scale bioreactors or disposal sites, all featuring relatively high concentrations of sulfate, and from natural environments in order to produce sulfide from gypsum using hydrogen as energy source. The properties of the eleven strains have been investigated and compared to these of three collection strains i.e. Desulfovibrio desulfuricans and vulgaris and Desulfotomaculum orientis. Particular attention was paid to the absolute and relative sulfide production rate and to the hydrogen sulfide inhibition level. By comparison to the collection strains, a 75 % higher production rate and a hydrogen sulfide inhibition level about twice higher i.e. 25.1 mM have been achieved with strains isolated from sulfate-rich environments. The strain selection, particularly from sulfate-rich environments, should be considered as an optimization factor for the sulfate reduction processes. [less ▲]Detailed reference viewed: 18 (12 ULg)
La bibliothèque, partenaire du projet pédagogique : compte-rendu des ateliers francophones sur la formation documentaire, Gembloux 13-15 octobre 1997
Pochet, Bernard ; Thirion, Paul
Book published by CIUF (1998)Detailed reference viewed: 124 (31 ULg)
Evidence For The Occurrence Of Endophytic Prokaryotic Contaminants In Micropropogated Plantlets Of Prunus Cerasus Cv. 'Montmorency'
; Lepoivre, Philippe ;
in Plant Cell, Tissue & Organ Culture (1998), 52(1-2),Detailed reference viewed: 5 (0 ULg)
Arbres fruitiers et virus: le diagnostic moleculaire.
; Lepoivre, Philippe
in Agricontact (1998), (302),Detailed reference viewed: 11 (3 ULg)
Development of routine dtection tests using PCR for certification
; ; et al
in Med. Fac. Landbouw. Univ. Gent, 63/4b, 1998 (1998)Detailed reference viewed: 7 (0 ULg)
Sensitive detection of apple stem grooving and apple stem pitting viruses from infected apple trees by RT-PCR
; ; Rufflard, Gladys et al
in Acta Horticulturae (1998), 472Detailed reference viewed: 10 (0 ULg)
Development of colorimetric RT-PCR tets for apple stem grooving virus detection in apple trees
; ; et al
in Acta Horticulturae (1998), 472Detailed reference viewed: 5 (0 ULg)
Données nouvelles sur la laumontite de la zone métamorphique de Libramont, Belgique.
in Geologica Belgica (1998), 1Detailed reference viewed: 10 (4 ULg)
The transverse cross section in exclusive vector meson production
Cudell, Jean-René ;
in Fried, H. M.; Mueller, B. (Eds.) Paris 1998, Quantum chromodynamics (1998)Detailed reference viewed: 6 (0 ULg)
Alternative oxidase in the branched mitochondrial respiratory network: an overview on structure, function, regulation, and role.
Sluse, Francis ;
in Brazilian Journal of Medical and Biological Research = Revista Brasileira de Pesquisas Medicas e Biologicas (1998), 31
Plants and some other organisms including protists possess a complex branched respiratory network in their mitochondria. Some pathways of this network are not energy-conserving and allow sites of energy ... [more ▼]
Plants and some other organisms including protists possess a complex branched respiratory network in their mitochondria. Some pathways of this network are not energy-conserving and allow sites of energy conservation to be bypassed, leading to a decrease of the energy yield in the cells. It is a challenge to understand the regulation of the partitioning of electrons between the various energy-dissipating and -conserving pathways. This review is focused on the oxidase side of the respiratory chain that presents a cyanide-resistant energy-dissipating alternative oxidase (AOX) besides the cytochrome pathway. The known structural properties of AOX are described including transmembrane topology, dimerization, and active sites. Regulation of the alternative oxidase activity is presented in detail because of its complexity. The alternative oxidase activity is dependent on substrate availability: total ubiquinone concentration and its redox state in the membrane and O2 concentration in the cell. The alternative oxidase activity can be long-term regulated (gene expression) or short-term (post-translational modification, allosteric activation) regulated. Electron distribution (partitioning) between the alternative and cytochrome pathways during steady-state respiration is a crucial measurement to quantitatively analyze the effects of the various levels of regulation of the alternative oxidase. Three approaches are described with their specific domain of application and limitations: kinetic approach, oxygen isotope differential discrimination, and ADP/O method (thermokinetic approach). Lastly, the role of the alternative oxidase in non-thermogenic tissues is discussed in relation to the energy metabolism balance of the cell (supply in reducing equivalents/demand in energy and carbon) and with harmful reactive oxygen species formation. [less ▲]Detailed reference viewed: 6 (1 ULg)
Impairment of the mitochondrial electron chain transport prevents NF-kappa B activation by hydrogen peroxide.
; ; et al
in Free Radical Biology & Medicine (1998), 25(1), 104-112
A large body of work has been devoted to mechanisms leading to the activation of the transcription factor NF-kappa B in various cell types. Several studies have indicated that NF-kappa B activation by ... [more ▼]
A large body of work has been devoted to mechanisms leading to the activation of the transcription factor NF-kappa B in various cell types. Several studies have indicated that NF-kappa B activation by numerous stimuli depends on the intracellular generation of reactive oxygen species (ROS). In this report, we first demonstrated that inhibition of the electron transport chain by either rotenone or antimycine A gave rise to dose-dependent inhibition of NF-kappa B translocation induced by 150 microM of hydrogen peroxide (H2O2). Conversely, the impairment of the mitochondrial respiratory chain did not affect T lymphocyte treatment by TNF-alpha (tumor necrosis factor alpha) or pre-B lymphocyte treatment with LPS (lipopolysaccharide). We also showed that oligomycine which inhibits ATP synthase and FCCP, which uncouples respiration also led to dose-dependent inhibition of NF-kappa B activation by H2O2. All these inhibitors were also shown to inhibit mitochondrial respiration in lymphocytes assessed by oxygen consumption. Although only a transient drop in ATP concentration was observed when lymphocytes were treated by H2O2, this effect was remarkably reinforced in the presence of oligomycine demonstrating the crucial role of ATP in the signal transduction pathway induced by H2O2. [less ▲]Detailed reference viewed: 8 (1 ULg)
Free fatty acids regulate the uncoupling protein and alternative oxidase activities in plant mitochondria.
Sluse, Francis ; ; et al
in FEBS Letters (1998), 433
Two energy-dissipating systems, an alternative oxidase and an uncoupling protein, are known to exist in plant mitochondria. In tomato fruit mitochondria linoleic acid, a substrate for the uncoupling ... [more ▼]
Two energy-dissipating systems, an alternative oxidase and an uncoupling protein, are known to exist in plant mitochondria. In tomato fruit mitochondria linoleic acid, a substrate for the uncoupling protein, inhibited the alternative oxidase-sustained respiration and decreased the ADP/O ratio to the same value regardless of the level of alternative oxidase activity. Experiments with varying concentrations of linoleic acid have shown that inhibition of the alternative oxidase is more sensitive to the linoleic acid concentration than the uncoupling protein activation. It can be proposed that these dissipating systems work sequentially during the life of the plant cell, since a high level of free fatty acid-induced uncoupling protein activity excludes alternative oxidase activity. [less ▲]Detailed reference viewed: 6 (1 ULg)
Generation of superoxide anion by mitochondria and impairment of their functions during anoxia and reoxygenation in vitro.
; ; Sluse, Francis
in Free Radical Biology & Medicine (1998), 25
A small portion of the oxygen consumed by aerobic cells is converted to superoxide anion at the level of the mitochondrial respiratory chain. If produced in excess, this harmful radical is considered to ... [more ▼]
A small portion of the oxygen consumed by aerobic cells is converted to superoxide anion at the level of the mitochondrial respiratory chain. If produced in excess, this harmful radical is considered to impair cellular structures and functions. Damage at the level of mitochondria have been reported after ischemia and reperfusion of organs. However, the complexity of the in vivo system prevents from understanding and describing precise mechanisms and locations of mitochondrial impairment. An in vitro model of isolated-mitochondria anoxia-reoxygenation is used to investigate superoxide anion generation together with specific damage at the level of mitochondrial oxidative phosphorylation. Superoxide anion is detected by electron paramagnetic resonance spin trapping with POBN-ethanol. Mitochondrial respiratory parameters are calculated from oxygen consumption traces recorded with a Clark electrode. Respiring mitochondria produce superoxide anion in unstressed conditions, however, the production is raised during postanoxic reoxygenation. Several respiratory parameters are impaired after reoxygenation, as shown by decreases of phosphorylating and uncoupled respiration rates and of ADP/O ratio and by increase of resting respiration. Partial protection of mitochondrial function by POBN suggests that functional damage is related and secondary to superoxide anion production by the mitochondria in vitro. [less ▲]Detailed reference viewed: 22 (3 ULg)
Linoleic acid-induced activity of plant uncoupling mitochondrial protein in purified tomato fruit mitochondria during resting, phosphorylating, and progressively uncoupled respiration.
; ; et al
in Journal of Biological Chemistry (1998), 273(52), 34882-34886
An uncoupling protein was recently discovered in plant mitochondria and demonstrated to function similarly to the uncoupling protein of brown adipose tissue. In this work, green tomato fruit mitochondria ... [more ▼]
An uncoupling protein was recently discovered in plant mitochondria and demonstrated to function similarly to the uncoupling protein of brown adipose tissue. In this work, green tomato fruit mitochondria were purified on a self-generating Percoll gradient in the presence of 0.5% bovine serum albumin to deplete mitochondria of endogenous free fatty acids. The uncoupling protein activity was induced by the addition of linoleic acid during the resting state, and in the progressively uncoupled state, as well as during phosphorylating respiration in the presence of benzohydroxamic acid, an inhibitor of the alternative oxidase and with succinate (+ rotenone) as oxidizable substrate. Linoleic acid strongly stimulated the resting respiration in fatty acid-depleted mitochondria but had no effect on phosphorylating respiration, suggesting no activity of the uncoupling protein in this respiratory state. Progressive uncoupling of state 4 respiration decreased the stimulation by linoleic acid. The similar respiratory rates in phosphorylating and fully uncoupled respiration in the presence and absence of linoleic acid suggested that a rate-limiting step on the dehydrogenase side of the respiratory chain was responsible for the insensitivity of phosphorylating respiration to linoleic acid. Indeed, the ADP/O ratio determined by ADP/O pulse method was decreased by linoleic acid, indicating that uncoupling protein was active during phosphorylating respiration and was able to divert energy from oxidative phosphorylation. Moreover, the respiration rates appeared to be determined by membrane potential independently of the presence of linoleic acid, indicating that linoleic acid-induced stimulation of respiration is due to a pure protonophoric activity without any direct effect on the electron transport chain. [less ▲]Detailed reference viewed: 4 (0 ULg)