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See detailEffect of antiepileptic drugs and reactive oxygen species on folate receptor 1 (FOLR1)-dependent 5-methyltetrahydrofolate transport.
Opladen, Thomas; Blau, Nenad; RAMAEKERS, Vincent ULiege

in Molecular Genetics & Metabolism (2010), 101(1), 48-54

Metabolic breakdown of valproate (VPA), carbamazepine (CBZ) and phenytoin (PHT) by the cytochrome P450 pathway generates toxic drug intermediates and reactive oxygen species (ROS). This mechanism has been ... [more ▼]

Metabolic breakdown of valproate (VPA), carbamazepine (CBZ) and phenytoin (PHT) by the cytochrome P450 pathway generates toxic drug intermediates and reactive oxygen species (ROS). This mechanism has been suspected to play a role in the pathogenesis of secondary cerebral folate deficiency (CFD). Using KB-cell cultures, highly expressing the folate receptor 1 (FOLR1), the effect of antiepileptic drugs (AEDs) and reactive oxygen species (ROS) on the FOLR1 dependent 5-methyltetrahydrofolate (MTHF) uptake was studied. MTHF uptake is time and concentration dependent and shows saturation kinetics. At physiological MTHF concentrations the high-affinity FOLR1 represents the predominant mechanism for cellular incorporation, while at high MTHF concentrations other transport mechanisms participate in folate uptake. Exposure to PHT for more than 8h led to a higher MTHF uptake and decreased cell count, whereas MTHF uptake remained unaltered by VPA and CBZ. However, exposure to superoxide and hydrogen peroxide radicals significantly decreased cellular MTHF uptake. By specific elimination and downregulation of FOLR1 using phosphatidyl-inositol-specific phospholipase C (PIPLC) and siRNA silencing, it was shown that ROS not only inhibited FOLR1 mediated MTHF uptake but also affected all other mechanisms of membrane-mediated MTHF uptake. Generation of ROS with the use of AED might therefore provide an additional explanation for the disturbed folate transfer across the blood-CSF barrier in patients with CFD. [less ▲]

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See detailEukaryotic algae: where lies the diversity of oxygenic photosynthesis.
Cardol, Pierre ULiege; Franck, Fabrice ULiege

in Photosynthesis Research (2010), 106(1-2), 1-2

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See detailConcepts actuels de l'hyperaldostéronisme primaire
Vroonen, Laurent ULiege; Krzesinski, Jean-Marie ULiege; Hamoir, Etienne ULiege et al

in Revue Médicale de Liège (2010), 65(10), 583-587

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See detailElectrochromism: a useful probe to study algal photosynthesis.
Bailleul, Benjamin; Cardol, Pierre ULiege; Breyton, Cecile et al

in Photosynthesis Research (2010), 106(1-2), 179-89

In photosynthesis, electron transfer along the photosynthetic chain results in a vectorial transfer of protons from the stroma to the lumenal space of the thylakoids. This promotes the generation of an ... [more ▼]

In photosynthesis, electron transfer along the photosynthetic chain results in a vectorial transfer of protons from the stroma to the lumenal space of the thylakoids. This promotes the generation of an electrochemical proton gradient (Deltamu(H)(+)), which comprises a gradient of electric potential (DeltaPsi) and of proton concentration (DeltapH). The Deltamu(H)(+) has a central role in the photosynthetic process, providing the energy source for ATP synthesis. It is also involved in many regulatory mechanisms. The DeltapH modulates the rate of electron transfer and triggers deexcitation of excess energy within the light harvesting complexes. The DeltaPsi is required for metabolite and protein transport across the membranes. Its presence also induces a shift in the absorption spectra of some photosynthetic pigments, resulting in the so-called ElectroChromic Shift (ECS). In this review, we discuss the characteristic features of the ECS, and illustrate possible applications for the study of photosynthetic processes in vivo. [less ▲]

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See detailF1FO ATP synthase mutants in Chlamydomonas: Stability and oligomycin resistance mediated by atypical Asa7 protein; interaction between chloroplastic and mitochondrial bioenergetics
Lapaille, Marie ULiege; Escobar-Ramírez, Adelma; Degand, Hervé et al

in Biochimica et Biophysica Acta (BBA) - Bioenergetics (2010), 1797(Supplement 1), 29

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See detailModeling of InSAR displacements related with the January 2002 eruption of Nyiragongo volcano
Wauthier, Christelle ULiege; Cayol, Valérie; Kervyn, François et al

in ECGS Blue Books (2010)

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See detailGene expression and genetic analysis during higher plants embryogenesis
Abid, Ghassen ULiege; Jaquemin, Jean-Marie; Sassi, Khaled et al

in Biotechnologie, Agronomie, Société et Environnement = Biotechnology, Agronomy, Society and Environment [=BASE] (2010), 14(4), 667-680

This review describes and discusses recent attempts to analyze the embryogenesis process in higher plants, through combination of descriptive, experimental, and genetic approach. Analysis of gene ... [more ▼]

This review describes and discusses recent attempts to analyze the embryogenesis process in higher plants, through combination of descriptive, experimental, and genetic approach. Analysis of gene expression profiles has permitted to build hypothesis concerning the induced mechanisms in early phases of embryogenesis in higher plants. Such mechanisms involve specific transcriptional and post-transcriptional regulatory pathways as well as diverse signal transduction processes at each stage of plant development. [less ▲]

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See detailLEGAL CERTAINTY v LEGAL PRECISION. Some thoughts on comparative law
Gerkens, Jean-François ULiege

in Fundamina : A Journal of Legal History (2010), 16-1(2010), 121-129

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See detailEDUCATE State of the Art Academic Curricula and Conditions for Registration
Altomonte, Sergio; Attia, Shady ULiege; Herde, André et al

Report (2010)

The analysis and consolidation of the state of the art of environmental sustainability in higher education and in the conditions for accreditation and professional registration has constituted one of the ... [more ▼]

The analysis and consolidation of the state of the art of environmental sustainability in higher education and in the conditions for accreditation and professional registration has constituted one of the tasks of the 6-month long Work Package 2 - the first stage of development of EDUCATE - so as to build a comprehensive picture in terms of integration of environmental design and energy efficiency in current academic pedagogies and in the criteria that control access to the practice of architecture. [less ▲]

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See detailLa formation: un outil indispensable pour l'ingénieur
Courard, Luc ULiege; Michel, Frédéric ULiege

Article for general public (2010)

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See detailOrgan failure and tight glycemic control in the SPRINT study.
Chase, J Geoffrey; Pretty, Christopher G; Pfeifer, Leesa et al

in Critical Care (2010), 14(4), 154

INTRODUCTION: Intensive care unit mortality is strongly associated with organ failure rate and severity. The sequential organ failure assessment (SOFA) score is used to evaluate the impact of a successful ... [more ▼]

INTRODUCTION: Intensive care unit mortality is strongly associated with organ failure rate and severity. The sequential organ failure assessment (SOFA) score is used to evaluate the impact of a successful tight glycemic control (TGC) intervention (SPRINT) on organ failure, morbidity, and thus mortality. METHODS: A retrospective analysis of 371 patients (3,356 days) on SPRINT (August 2005 - April 2007) and 413 retrospective patients (3,211 days) from two years prior, matched by Acute Physiology and Chronic Health Evaluation (APACHE) III. SOFA is calculated daily for each patient. The effect of the SPRINT TGC intervention is assessed by comparing the percentage of patients with SOFA </=5 each day and its trends over time and cohort/group. Organ-failure free days (all SOFA components </=2) and number of organ failures (SOFA components >2) are also compared. Cumulative time in 4.0 to 7.0 mmol/L band (cTIB) was evaluated daily to link tightness and consistency of TGC (cTIB >/=0.5) to SOFA </=5 using conditional and joint probabilities. RESULTS: Admission and maximum SOFA scores were similar (P = 0.20; P = 0.76), with similar time to maximum (median: one day; IQR: 13 days; P = 0.99). Median length of stay was similar (4.1 days SPRINT and 3.8 days Pre-SPRINT; P = 0.94). The percentage of patients with SOFA </=5 is different over the first 14 days (P = 0.016), rising to approximately 75% for Pre-SPRINT and approximately 85% for SPRINT, with clear separation after two days. Organ-failure-free days were different (SPRINT = 41.6%; Pre-SPRINT = 36.5%; P < 0.0001) as were the percent of total possible organ failures (SPRINT = 16.0%; Pre-SPRINT = 19.0%; P < 0.0001). By Day 3 over 90% of SPRINT patients had cTIB >/=0.5 (37% Pre-SPRINT) reaching 100% by Day 7 (50% Pre-SPRINT). Conditional and joint probabilities indicate tighter, more consistent TGC under SPRINT (cTIB >/=0.5) increased the likelihood SOFA </=5. CONCLUSIONS: SPRINT TGC resolved organ failure faster, and for more patients, from similar admission and maximum SOFA scores, than conventional control. These reductions mirror the reduced mortality with SPRINT. The cTIB >/=0.5 metric provides a first benchmark linking TGC quality to organ failure. These results support other physiological and clinical results indicating the role tight, consistent TGC can play in reducing organ failure, morbidity and mortality, and should be validated on data from randomised trials. [less ▲]

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See detailConsolidation institutionnelle et démocratisation en RDC
Kabamba, Bob ULiege

in Revue de la Faculté de Droit de l'Université de Liège (2010)

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See detailGrupurile informale : primii pasi in actiunea colectiva
Teclu, Codrat Alin ULiege

in Lambru, Mihaela; Vamescu, Ancuta; Kivu, Mircea (Eds.) Romania 2010 : Sectorul neguvernamental - profil, tendinte, provocari (2010)

L'ouvrage se propose de dresser un portrait quantitatif et qualitatif du secteur non-gouvernemental roumain 10 ans après la dernière grande enquête sur le sujet. Les principaux défis des ONG sont ... [more ▼]

L'ouvrage se propose de dresser un portrait quantitatif et qualitatif du secteur non-gouvernemental roumain 10 ans après la dernière grande enquête sur le sujet. Les principaux défis des ONG sont également abordés. Notre contribution sur les groupes informels de citoyens se trouve en pp. 90-93. [less ▲]

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See detailEtude des potentialités de la vision artificielle pour la reconnaissance optique des semences immatures de chicorée industrielle (Cichorium intybus L.)
Ooms, David ULiege; Destain, Marie-France ULiege

in Biotechnologie, Agronomie, Société et Environnement = Biotechnology, Agronomy, Society and Environment [=BASE] (2010), 14(1), 253-263

La production commerciale de semences de chicorée industrielle (Cichorium intybus L.) implique l'élimination des semences (cypsèles) non viables de la récolte à l’aide de méthodes non destructives. Dans ... [more ▼]

La production commerciale de semences de chicorée industrielle (Cichorium intybus L.) implique l'élimination des semences (cypsèles) non viables de la récolte à l’aide de méthodes non destructives. Dans ce cadre, deux techniques basées sur la vision artificielle sont étudiées pour reconnaître les semences non viables : la vision couleur et l’imagerie fluorescente. L’analyse de 1500 semences de la variété Nausica en vision en couleur ne permet de détecter que les semences desséchées ou non développées. Ceci est attribué à la très grande variabilité de la couleur, de la forme et de la texture des semences testées. L’imagerie fluorescente est alors envisagée, en vue d’analyser sur les différentes parties des semences (le péricarpe, la zone d’émergence de l’apex radiculaire et les pappi) la répartition de la chlorophylle, qui est un indicateur de la maturité des semences. La faible teneur en chlorophylle et la grande quantité de semences à trier imposent d’utiliser un dispositif de mesure très sensible. Un dispositif d'imagerie de la fluorescence chlorophyllienne de semences est ainsi proposé. Sa particularité consiste à pouvoir modifier le spectre de la source lumineuse (dans le but d’optimiser la sensibilité) et à enregistrer l’évolution de la répartition de la fluorescence en fonction du temps. Le dispositif fournit des images de la répartition de la fluorescence chlorophyllienne sur les cypsèles de chicorée. Il pourrait permettre de réaliser des mesures à plus grande échelle, reliant les attributs de fluorescence avec le pouvoir germinatif et l’état de maturité des semences. [less ▲]

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See detailSprouty1, a new target of the angiostatic agent 16K prolactin, negatively regulates angiogenesis
Sabatel, Céline ULiege; Cornet, Anne ULiege; Tabruyn, Sébastien ULiege et al

in Molecular Cancer (2010), 9(1), 231

BACKGROUND:Disorganized angiogenesis is associated with several pathologies, including cancer. The identification of new genes that control tumor neovascularization can provide novel insights for future ... [more ▼]

BACKGROUND:Disorganized angiogenesis is associated with several pathologies, including cancer. The identification of new genes that control tumor neovascularization can provide novel insights for future anti-cancer therapies. Sprouty1 (SPRY1), an inhibitor of the MAPK pathway, might be one of these new genes. We identified SPRY1 by comparing the transcriptomes of untreated endothelial cells with those of endothelial cells treated by the angiostatic agent 16K prolactin (16K hPRL). In the present study, we aimed to explore the potential function of SPRY1 in angiogenesis.RESULTS:We confirmed 16K hPRL induced up-regulation of SPRY1 in primary endothelial cells. In addition, we demonstrated the positive SPRY1 regulation in a chimeric mouse model of human colon carcinoma in which 16K hPRL treatment was shown to delay tumor growth. Expression profiling by qRT-PCR with species-specific primers revealed that induction of SPRY1 expression by 16K hPRL occurs only in the (murine) endothelial compartment and not in the (human) tumor compartment. The regulation of SPRY1 expression was NF-kappaB dependent. Partial SPRY1 knockdown by RNA interference protected endothelial cells from apoptosis as well as increased endothelial cell proliferation, migration, capillary network formation, and adhesion to extracellular matrix proteins. SPRY1 knockdown was also shown to affect the expression of cyclinD1 and p21 both involved in cell-cycle regulation. These findings are discussed in relation to the role of SPRY1 as an inhibitor of ERK/MAPK signaling and to a possible explanation of its effect on cell proliferation.CONCLUSIONS:Taken together, these results suggest that SPRY1 is an endogenous angiogenesis inhibitor. [less ▲]

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See detailEffets de la substitution du tourteau d’arachide par la fève de coton conventionnel en production de poulet de chair au Sénégal
Diaw, Mamadou Tandiang; Dieng, Abdoulaye; Mergeai, Guy ULiege et al

in Tropicultura (2010), 28(3), 139-147

A study has been conducted on 400 chicks to evaluate conventional cottonseed kernels on broilers production. Groundnut cake has been substituted by cottonseed kernels at 0, 25, 50 and 75% levels ... [more ▼]

A study has been conducted on 400 chicks to evaluate conventional cottonseed kernels on broilers production. Groundnut cake has been substituted by cottonseed kernels at 0, 25, 50 and 75% levels. Cottonseed kernel incorporation decreased significantly (p< 0.001) the growing parameters, feed intake, feed efficiency and nutrients digestibility of the experimental diets. This reduction was proportional to the level of substitution. The study suggests that only the level of gossypol in cottonseed kernels limits their incorporation in broilers diets and that this by-product should be excluded from the formulation in broilers production or that its level of incorporation should be limited to a maximal level of 10% when production duration is not a constraint for breeders. [less ▲]

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See detailLymphangiogenesis: in vitro and in vivo models
Bruyère, Françoise; Noël, Agnès ULiege

in FASEB Journal (2010), 24(1), 8-21

Lymphangiogenesis, the formation of new lymphatic vessels from preexisting ones, is an important biological process associated with diverse pathologies, such as metastatic dissemination and graft ... [more ▼]

Lymphangiogenesis, the formation of new lymphatic vessels from preexisting ones, is an important biological process associated with diverse pathologies, such as metastatic dissemination and graft rejection. In addition, lymphatic hypoplasia characterizes lymphedema, usually a progressive and lifelong condition for which no curative treatment exists. Much progress has been made in recent years in identifying molecules specifically expressed on lymphatic vessels and in the setting up of in vitro and in vivo models of lymphangiogenesis. These new tools rapidly provided an abundance of information on the mechanisms underlying lymphatic development and the progression of diseases associated with lymphatic dysfunction. In this review, we describe the common in vitro and in vivo models of lymphangiogenesis that have proven suitable for investigating lymphatic biology and the interactions occurring between lymphatic vessels and other cells, such as immune cells and cancer cells. Their rationales and limitations are discussed and illustrated by the most informative findings obtained with them. [less ▲]

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