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See detailNovel types of mutation responsible for the dermatosparactic type of Ehlers-Danlos syndrome (Type VIIC) and common polymorphisms in the ADAMTS2 gene.
Colige, Alain ULg; Nuytinck, Lieve; Hausser, Ingrid et al

in The Journal of investigative dermatology (2004), 123(4), 656-63

Ehlers-Danlos syndrome (EDS) type VIIC, or dermatosparactic type, is a recessively inherited connective tissue disorder characterized, among other symptoms, by an extreme skin fragility resulting from ... [more ▼]

Ehlers-Danlos syndrome (EDS) type VIIC, or dermatosparactic type, is a recessively inherited connective tissue disorder characterized, among other symptoms, by an extreme skin fragility resulting from mutations inactivating ADAMTS-2, an enzyme excising the aminopropeptide of procollagens type I, II, and III. All previously described mutations create premature stop codons leading to a marked reduction in the level of mRNA. In this study, we analyzed the ADAMTS2 cDNA sequences from five patients displaying clinical and/or biochemical features consistent with a diagnosis of either typical or potentially mild form of EDS type VIIC. Three different alterations were detected in the two patients with typical EDS type VIIC. The first patient was homozygous for a genomic deletion causing an in-frame skipping of exons 3-5 in the transcript. In the second patient, the allele inherited from the mother lacks exon 3, generating a premature stop codon, whereas the paternal allele has a genomic deletion resulting in an in-frame skipping of exons 14-16 at the mRNA level. Although the exons 3-5 or 14-16 encode protein domains that have not been previously recognized as crucial for ADAMTS-2 activity, the aminoprocollagen processing was strongly impaired in vitro and in vivo, providing evidence for the requirement of these domains for proper enzyme function. The three other patients with a phenotype with some resemblance to EDS type VIIC only had silent and functionally neutral variations also frequently found in a normal population. [less ▲]

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See detailExperimental study of the charring rate of tropical hardwoods
Njankouo, Jacques Michel; Dotreppe, Jean-Claude ULg; Franssen, Jean-Marc ULg

in Fire & Materials (2004), 28(1, JAN-FEB), 15-24

This paper describes an experimental investigation of the charring rates of timber. An experimental procedure was designed for assessing the charring rate of 20 specimens exposed to fire in a single test ... [more ▼]

This paper describes an experimental investigation of the charring rates of timber. An experimental procedure was designed for assessing the charring rate of 20 specimens exposed to fire in a single test, with one-dimensional heat transfer conditions. Each test specimen was manufactured by gluing seven laminates together. Four thermocouples were inserted at different depths in four different laminates located in the middle of the test specimen. The test was conducted using a gas-fired furnace and specimens were exposed to the standard ISO 834 fire. In order to evaluate the fire performance of tropical hardwoods, seven different species with densities ranging from 500 to 1000 kg/m(3) were used. For the purpose of verifying the experimental procedure against existing data, two softwood species (spruce and fir) and one European hardwood species (oak) were also examined. Experimental results indicate that the test method and procedure can be used for assessing the charring rate of timber both for softwood and for hardwood species. They also show that the density of wood significantly affects the charring rate and that the values recommended in Eurocode 5 for high densities are somewhat too conservative. Copyright (C) 2004 John Wiley Sons, Ltd. [less ▲]

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See detailTreillis des sous-algèbres et opérateurs sur les MV-algèbres
Teheux, Bruno ULg

Master of advanced studies dissertation (2004)

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See detailIn vitro evaluation of glutathione peroxidase (GPx)-like activity and antioxidant properties of some Ebselen analogues
Mouithys-Mickalad, Ange ULg; Mareque-Faez, Juan; Chistiaens, U. et al

in Redox Report : Communications in Free Radical Research (2004), 9(2), 81-87

Four analogues of Ebselen were synthesized and their glutathione peroxidase activity and antioxidant property evaluated and compared to Ebselen. Among the studied compounds, only diselenide [3] exhibited ... [more ▼]

Four analogues of Ebselen were synthesized and their glutathione peroxidase activity and antioxidant property evaluated and compared to Ebselen. Among the studied compounds, only diselenide [3] exhibited both glutathione peroxidase activity and radical-scavenging capability. Compounds [3] and [4] showed a strong inhibitory effect (53% and 43%, respectively) on the lipid peroxidation of linoleic acid compared to Ebselen and selenide derivatives ([1] and [2]) which were less active (28%, 26% and 18% inhibition, respectively). A concentration-dependent inhibitory effect was also found in the model of the formation of ABTS*+ radical cation: 65% and 89% inhibition for compound [3] at 10(-4) M and 5 x 10(-5) M, respectively, and 68% and 90% for compound [4], compared to 14% and 52% inhibition for Ebselen and the diselenides [1] and [2] (29%, 46% and 45%, 68%, respectively). By EPR spin trapping technique, the following inhibitory profile of the Ebselen analogues was observed towards the formation of thiyl radicals: Ebselen = [3]>[1]>[2]>[4]. Studies with compound [3] are in progress on oxidative stress cell models. [less ▲]

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See detailAgir de manière socialement responsable: la richesse d'une approche par les parties prenantes
Xhauflair, Virginie ULg

in Entreprise Ethique (2004), 21(octobre), 105-111

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See detailAnalyse des prix de ventes des bois en forêts publiques
Sanchez Christine; Hebert, Jacques ULg; Rondeux, Jacques ULg

in Forêt Wallonne (2004), 73

La détermination des prix unitaires en euro/m³ reste très empirique et est souvent basée sur un nombre réduit de lots réputés représentatifs. La méthode proposée utilise des outils statistiques qui ... [more ▼]

La détermination des prix unitaires en euro/m³ reste très empirique et est souvent basée sur un nombre réduit de lots réputés représentatifs. La méthode proposée utilise des outils statistiques qui valorisent au maximum les informations disponibles. Elle a été intégrée dans une application informatique à la disposition des gestionnaires de la forêt publique. [less ▲]

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See detailConcomitant coronary and carotid artery surgery: operative outcome and long-term results
Kolh, Philippe ULg; Tchana-Sato, Vincent ULg; Honoré, Charles ULg et al

in European Heart Journal (2004), 25(Suppl. S), 360-361

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See detailSustained correction of B-cell development and function in a murine model of X-linked agammaglobulinemia (XLA) using retroviral-mediated gene transfer
Yu, P. W.; Tabuchi, R. S.; Kato, R. M. et al

in Blood (2004), 104(5), 1281-90

X-linked agammaglobulinemia (XLA) is a human immunodeficiency caused by mutations in Bruton tyrosine kinase (Btk) and characterized by an arrest in early B-cell development, near absence of serum ... [more ▼]

X-linked agammaglobulinemia (XLA) is a human immunodeficiency caused by mutations in Bruton tyrosine kinase (Btk) and characterized by an arrest in early B-cell development, near absence of serum immunoglobulin, and recurrent bacteria infections. Using Btk- and Tec-deficient mice (BtkTec-/-) as a model for XLA, we determined if Btk gene therapy could correct this disorder. Bone marrow (BM) from 5-fluorouracil (5FU)-treated BtkTec-/- mice was transduced with a retroviral vector expressing human Btk and transplanted into BtkTec-/- recipients. Mice engrafted with transduced hematopoietic cells exhibited rescue of both primary and peripheral B-lineage development, revocery of peritoneal B1 B cells, and correction of serum immunoglobulin M (IgM) and IgG3 levels. Gene transfer also restored T-independent type II immune responses, and B-cell antigen receptor (BCR) proliferative responses. B-cell progenitors derived from Btk-transduced stem cells exhibited higher levels of Btk expression than non-B cells; and marking studies demonstrated a selective advantage for Btk-transduced B-lineage cells. BM derived from primary recipients also rescued Btk-dependent function in secondary hosts that had received a transplant. Together, these data demonstrate that gene transfer into hematopoietic stem cells can reconstitute Btk-dependent B-cell development and function in vivo, and strongly support the feasibility of pursuing Btk gene transfer for XLA. [less ▲]

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See detailAffect et strategies d'approche/evitement
Grégoire, Christine; Dardenne, Benoît ULg

in Revue Internationale de Psychologie Sociale = International Review of Social Psychology (2004), 17(1), 111-144

Après avoir présenté certaines conceptions de l'approche et de l'évitement en tant que régulateurs du comportement humain, nous défendons l'idée que les individus ressentant des affects positifs ... [more ▼]

Après avoir présenté certaines conceptions de l'approche et de l'évitement en tant que régulateurs du comportement humain, nous défendons l'idée que les individus ressentant des affects positifs développent une stratégie d'approche en situation sociale. Sur base de la théorie du «feeling-as-information» (Schwarz & Clore, 1988), l'affect peut être vu comme une information contextuelle sur l'environnement. L'information selon laquelle l'environnement ne pose pas de problème conduit les individus ressentant des affects positifs à adopter un comportement adapté vis-à-vis d'autrui et des situations sociales, alors que l'information selon laquelle l'environnement est menaçant conduit les individus ressentant des affects négatifs à davantage éviter autrui et les situations sociales. Nous illustrons notre propos par des recherches traitant de la quantité et de la qualité des activités sociales, ainsi que des comportements prosociaux. [less ▲]

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See detailSelf-assembling of poly(-caprolactone)-b-poly(ethylene oxide) diblock copolymers in aqueous solution and at the silica–water interface
Leyh, Bernard ULg; Vangeyte, Patrick; Heinrich, M. et al

in Physica B: Condensed Matter (2004), 350(1-3, suppl. 1), 901-904

Small-angle neutron scattering is used to investigate the self-assembling behaviour of poly( ε-caprolactone)-b-poly(ethylene oxide) diblock copolymers with various block lengths (i) in aqueous solution ... [more ▼]

Small-angle neutron scattering is used to investigate the self-assembling behaviour of poly( ε-caprolactone)-b-poly(ethylene oxide) diblock copolymers with various block lengths (i) in aqueous solution, (ii) in aqueous solution with the addition of sodium dodecyl sulphate (SDS) and (iii) at the silica-water interface. Micelles are observed under our experimental conditions due to the very small critical micellar concentration of these copolymers (0.01 g/l). The poly( ε-caprolactone) core is surrounded by a poly(ethylene oxide) corona. The micellar form factors have been measured at low copolymer concentrations (0.2 wt%) under selected contrast matching conditions. The data have been fitted to various analytical models to extract the micellar core and corona sizes. SDS is shown to induce partial micelle disruption together with an increase of the poly(ethylene oxide) corona extension from 25% (without SDS) to 70% (with SDS) of a completely extended PEOi u chain. Our data at the silica-water interface are compatible with the adsorption of micelles. [less ▲]

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See detailFpvB, an alternative type I ferripyoverdine receptor of Pseudomonas aeruginosa.
Ghysels, Bart ULg; Dieu, Bui Thi Min; Beatson, Scott A. et al

in Microbiology (Reading, England) (2004), 150(Pt 6), 1671-80

Under conditions of iron limitation, Pseudomonas aeruginosa secretes a high-affinity siderophore pyoverdine to scavenge Fe(III) in the extracellular environment and shuttle it into the cell. Uptake of the ... [more ▼]

Under conditions of iron limitation, Pseudomonas aeruginosa secretes a high-affinity siderophore pyoverdine to scavenge Fe(III) in the extracellular environment and shuttle it into the cell. Uptake of the pyoverdine-Fe(III) complex is mediated by a specific outer-membrane receptor protein, FpvA (ferripyoverdine receptor). Three P. aeruginosa siderovars can be distinguished, each producing a different pyoverdine (type I-III) and a cognate FpvA receptor. Growth of an fpvA mutant of P. aeruginosa PAO1 (type I) under iron-limiting conditions can still be stimulated by its cognate pyoverdine, suggesting the presence of an alternative uptake route for type I ferripyoverdine. In silico analysis of the PAO1 genome revealed that the product of gene PA4168 has a high similarity with FpvA. Inactivation of PA4168 (termed fpvB) in an fpvA mutant totally abolished the capacity to utilize type I pyoverdine. The expression of fpvB is induced by iron limitation in Casamino acids (CAA) and in M9-glucose medium, but, unlike fpvA, not in a complex deferrated medium containing glycerol as carbon source. The fpvB gene was also detected in other P. aeruginosa isolates, including strains producing type II and type III pyoverdines. Inactivation of the fpvB homologues in these strains impaired their capacity to utilize type I ferripyoverdine as a source of iron. Accordingly, introduction of fpvB in trans restored the capacity to utilize type I ferripyoverdine. [less ▲]

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See detail"Rationes lacunae". Pourquoi aucune "vita" de Saint Lambert n'a-t-elle été imprimée au XVe siècle?
Adam, Renaud ULg

in Bulletin de la Société Royale Le Vieux-Liège (2004), 14(17), 509-519

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See detailLa video-capsule endoscopique: une nouvelle methode d'exploration du grele
Dupont, P.; Louis, Edouard ULg; Belaiche, Jacques ULg

in Revue Médicale de Liège (2004), 59(7-8, Jul-Aug), 445-50

Capsule endoscopy is a new technique of exploration which enables to visualize the entire small bowel. Occult or unexplained digestive bleeding currently constitutes on major indication of this technique ... [more ▼]

Capsule endoscopy is a new technique of exploration which enables to visualize the entire small bowel. Occult or unexplained digestive bleeding currently constitutes on major indication of this technique. The aim of this study is to report the results obtained among 15 patients investigated by capsule endoscopy and to discuss the place of this new technique in the small bowel exploration. [less ▲]

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See detailLes Stèles arabes du Musée du Cinquantenaire (Bruxelles)
Bauden, Frédéric ULg

in Bauden, Frédéric (Ed.) Ultra mare. Mélanges de langue arabe et d’islamologie offerts à Aubert Martin (2004)

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See detailReply to "Comment on 'Detuning effects in the one-photon mazer' "
Bastin, Thierry ULg; Martin, John ULg

in Physical Review. A (2004), 70

We refute in this Reply the criticisms made by Abdel-Aty. We show that none of them are founded and we demonstrate very explicitly what is wrong with the arguments developed by this author.

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See detailGlobalisation, gouvernance et logiques régionales dans les Amériques
Santander, Sébastian ULg

Book published by L'Harmattan (2004)

L'ouvrage se penche sur les intégrations régionales dans les Amériques. Il épingle aussi bien les groupements régionaux issus d'une initiative latino-américaine que ceux issus de Washington.

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See detailA quantitative point of view of the concept of vulnerability
Brouyère, Serge ULg

in Zwahlen, François (Ed.) Vulnerability and risk mapping for the protection of carbonate (karst) aquifers, COST620 final report (2004)

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