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Publications ORBi OA
Molecular size and symmetry of Pseudomonas aeruginosa catabolic ornithine carbamoyltransferase. An X-ray crystallography analysis.; ; Charlier, Paulette et alin Journal of Molecular Biology (1991), 220(1), 9-12 The catabolic ornithine carbamoyltransferase (EC 2.1.3.3) from Pseudomonas aeruginosa, that shows allosteric behaviour, and a mutant version of this enzyme has been crystallized in several different ... [more ▼] The catabolic ornithine carbamoyltransferase (EC 2.1.3.3) from Pseudomonas aeruginosa, that shows allosteric behaviour, and a mutant version of this enzyme has been crystallized in several different crystal forms. All of these have been characterized by X-ray diffraction methods. A 4.5 A resolution data set has been collected on a triclinic crystal. Analysis of the data using the self-rotation function shows that 12 monomers associate to form a particle with cubic 23 point group symmetry. [less ▲] Detailed reference viewed: 6 (1 ULg) Metrópoli real y aldea letrada: las luchas por la hegemonía literaria en la Ciudad de MéxicoVanden Berghe, Kristine ![]() in Schneier, Gabriela (Ed.) Consommation urbaine de produits culturels (1991) Detailed reference viewed: 14 (5 ULg) L'agriculture dans la Province du LuxembourgBurny, Philippe ![]() in Annales de Gembloux (1991), 97(1), 43-67 Detailed reference viewed: 5 (1 ULg) Choix de l'habitat par le lynx européen et par sa proie principale, le chevreuil, dans un milieu de moyenne montagne (massif vosgien). 1. le cas du chevreuil; ; Libois, Roland ![]() in Cahiers d'Ethologie (1991), 11(1), 51-66 Since 1983, 12 lynxes were reintroduced in the forests of Eastern France (massif des Vosges). They use their habitat in a patchy way. In this part, the authors deal with the ecological distridution of ... [more ▼] Since 1983, 12 lynxes were reintroduced in the forests of Eastern France (massif des Vosges). They use their habitat in a patchy way. In this part, the authors deal with the ecological distridution of their main prey, the roe deer. It is to see to what extent the prey distribution can influence the predator habits. Roe deer observations were made during 4 consecutive springs and 3 consecutive summers and related with the environmental characteristics of the forest plots: physiographical factors, vegetation type, vegetation structure, relative cover of vegetation layer... Eight discriminant analysis were performed. They suggest that in our study area, the roe deer spatial distribution is mainly influenced by the altitude and the non deciduous tree cover. Other factors of less importance are the global tree cover and the understory (scrub layer). Moreover it seems that the presence of the red deer can also influence the roe deer habitat utilization. [less ▲] Detailed reference viewed: 21 (4 ULg) Daily Seasonalities on the Brussels Spot Equity MarketCorhay, Albert ![]() in Cahiers Economiques de Bruxelles (1991), (132), 415-430 Detailed reference viewed: 14 (5 ULg) Mesure de la dénitrification biologique potentielle dans les sols à l'aide de l'acétylène.; Tychon, Bernard ; Vander Borght, Paul ![]() in Revue de l’Agriculture = Landbouwtijdschrift (1991), 44(4), 637-648 De nombreuses méthodes ont été établies pour quantifier et définir la cinétique de dénitrification mais elles se sont toutes confrontées à la difficulté de doser l'azote de l'air (N2) qui est l'élément ... [more ▼] De nombreuses méthodes ont été établies pour quantifier et définir la cinétique de dénitrification mais elles se sont toutes confrontées à la difficulté de doser l'azote de l'air (N2) qui est l'élément terminal de la chaîne de Payne (1973) : NO3 → NO2 → NO → N2O → N2. L'utilisation de l'acétylène a résolu ce problème en permettant l'inhibition de la réduction de l'oxyde nitreux (N2O) en N2, l'oxyde nitreux étant facilement dosable par chromatographie gazeuse. La technique utilisée lors de ce travail consiste à prélever un échantillon de sol dans l'horizon de surface. Après homogénéisation, il est placé dans des fioles à pénicilline hermétiques de 120 ml. Une préincubation de 50 h à 20-22° C sous anaérobiose engendre l'élimination des nitrates endogènes présents au départ dans le sol, ce qui permet par la suite d'ajouter des quantités précises de nitrates en vue d'effectuer des bilans de transformation du NO3 en N2O. Cette préincubation est suivie d'une incubation sous air, en présence d'acétylène (10 % vol/vol), à 20° C et à 100 % de saturation en eau. Sous ces conditions, la production de N-N2O et la disparition de N-NO3 sont mesurées, permettant ainsi de quantifier les bilans de transformation. En reportant les résultats obtenus pour 1 ha de sol (pour les 20 premiers cm), 12 kg d'azote sur les 20 présents au départ ont dénitrifié sur une période de 5 jours. De plus, cette disparition suit une cinétique à allure précise. Si ces estimations s'avéraient correspondre à la réalité, les mécanismes en cause seraient alors de la plus grande importance, tant pour l'agriculture en améliorant l'utilisation de l'azote, que pour l'environnement et la pollution de l'air et de l'eau. [less ▲] Detailed reference viewed: 18 (0 ULg) Transfert des nitrates et qualité des eaux souterraines en Lorraine belge.Debbaut, Vincent ; Tychon, Bernard ; Vander Borght, Paul ![]() in Vander Borght, Paul; Tychon, Bernard (Eds.) Gestion de l'azote agricole et qualité des eaux. Actes du séminaire spécialisé (1991) Detailed reference viewed: 12 (1 ULg) Pratique agricole en Lorraine belge et pollution des eaux par les nitrates.Tychon, Bernard ; Vander Borght, Paul ![]() in Harpes, J.-P. (Ed.) Actes du colloque Les problèmes environnementaux au Luxembourg et dans la grande région. (1991) Detailed reference viewed: 20 (0 ULg) Conformational analysis of beta and gamma lactam antibiotics; Dive, Georges ; Ghuysen, Jean-Marie ![]() in European Journal of Medicinal Chemistry (1991), 26(1), 43-50 Geometry optimization, superimposition searches and conformational analysis carried on several lactam antibiotics show that reactivity with the active-site serine penicillin-binding proteins is related to ... [more ▼] Geometry optimization, superimposition searches and conformational analysis carried on several lactam antibiotics show that reactivity with the active-site serine penicillin-binding proteins is related to a particular spatial disposition of 2 flanking functional groups - namely a C = O or C-OH on 1 side and a carboxylate on the other - with respect to the central scissile amide bond. Such a binding entity is found in one of the most stable conformers of the tripeptide diacetyl-L-Lys-D-Ala-D-Ala conferring substrate activity, and in benzylpenicillin, cephapyrin, thienamycin, gamma-lactam, the 6-spiro-epoxypenicillin S and in one epimer of lactivicin, conferring inactivating potency. This binding entity generates a particular electronic distribution and the fact that it is conserved in compounds belonging to very different chemical families strongly suggests that it is an important feature required for enzyme recognition. [less ▲] Detailed reference viewed: 37 (1 ULg) L'éducation des jeunes enfants au seuil de l'ère technologiquede Landsheere, Gilbert ![]() in Communication et Langages (1991), (89), 31-36 Detailed reference viewed: 2 (0 ULg) Blowout of Carotid Venous Patch AngioplastyVAN DAMME, Hendrik ; GRENADE, Thierry ; CREEMERS, Etienne et alin Annals of Vascular Surgery (1991), 5(6), 542-5 Two cases of vein patch blowout were observed five and seven days after carotid bifurcation endarterectomy with patch angioplasty. Both patients died in spite of emergency reoperation. One patient ... [more ▼] Two cases of vein patch blowout were observed five and seven days after carotid bifurcation endarterectomy with patch angioplasty. Both patients died in spite of emergency reoperation. One patient developed respiratory failure with subsequent fatal cardiac arrest seven days after reoperation; the other died of extensive hemispheric infarction on the fifth postoperative day. At reoperation both ruptures were found to be located in the middle of the patch whereas the suture lines were intact. Both patients were hypertensive. In the first case, an accessory saphenous vein retrieved from the calf had been the only venous material available for the patch, while the other patient had varicose veins in the contralateral leg. Pathology revealed central transmural tissue necrosis in one of the disrupted patches. A review of the literature regarding morphologic alterations of free vein grafts placed within the arterial circulation as well as hemodynamics in patched arterial segments may provide additional insight as to the inherent benefits and risks of vein patch angioplasty after carotid endarterectomy. When considering vein patch angioplasty, particular attention should be directed to the gross aspect of the vein to be used as well as to any antecedent history of phlebitis. [less ▲] Detailed reference viewed: 15 (0 ULg) Decision Trees and Transient Stability of Electric Power SystemsWehenkel, Louis ; Pavella, Mania ![]() in Automatica (1991), 27(1), 115-134 An inductive inference method for the automatic building of decision trees is investigated. Among its various tasks, the splitting and the stop splitting criteria successively applied to the nodes of a ... [more ▼] An inductive inference method for the automatic building of decision trees is investigated. Among its various tasks, the splitting and the stop splitting criteria successively applied to the nodes of a grown tree, are found to play a crucial role on its overall shape and performances. The application of this general method to transient stability is systematically explored. Parameters related to the stop splitting criterion, to the learning set and to the tree classes are thus considered, and their influence on the tree features is scrutinized. Evaluation criteria appropriate to assess accuracy are also compared. Various tradeoffs are further examined, such as complexity vs number of classes, or misclassification rate vs type of misclassification errors. Possible uses of the trees are also envisaged. Computational issues relating to the building and the use of trees are finally discussed. [less ▲] Detailed reference viewed: 20 (2 ULg) Sequence analysis of the ARG7 gene of Schizosaccharomyces pombe coding for argininosuccinate lyase. Expression of the gene in Saccharomyces cerevisiae.Loppes, Roland ; ; et alin Current Genetics (1991), 19(4), 255-60 The complete nucleotide sequence of the ARG7 gene, coding for argininosuccinate lyase (EC 4.3.2.1), in the fission yeast (Schizosaccharomyces pombe) has been determined. It consists of an open reading ... [more ▼] The complete nucleotide sequence of the ARG7 gene, coding for argininosuccinate lyase (EC 4.3.2.1), in the fission yeast (Schizosaccharomyces pombe) has been determined. It consists of an open reading frame of 461 codons. The deduced protein has a molecular weight of 51,200 Da. The gene is devoid of introns which is confirmed by the fact that it is expressed in Escherichia coli after spontaneous insertion of a bacterial sequence probably bearing a prokaryotic promoter. A perfect "TATA" box is found at -72 and the major transcription initiation site in Saccharomyces cerevisiae is located at -11 as shown by primer extension experiments. Comparison of the S. pombe lyase with related proteins from other organisms reveals an important degree of conservation except in the carboxyterminal part of the polypeptide. Additionally, a deletion removing 66 amino acids of the carboxy terminus yields an enzyme exhibiting some biological activity. A unique 1,500 b transcript was found in S. cerevisiae when the intact gene was present, but the deleted version of the gene gave rise to at least three transcripts of 1,800, 2,800 and 3,900 b. [less ▲] Variations saisonnières du régime alimentaire du chevreuil (Capreolus capreolus) en Haute Belgique; Libois, Roland ![]() in Cahiers d'Ethologie (1991), 11(1), 17-30 In Belgium, information related to the deer food are quite uncommon and, as far as the roe deer is concerned, restricted to the hunting season (autumn). Our study was conducted in two areas of southern ... [more ▼] In Belgium, information related to the deer food are quite uncommon and, as far as the roe deer is concerned, restricted to the hunting season (autumn). Our study was conducted in two areas of southern Belgium. The first, in Hotton (50°15 N, 5°27E) is a mixed oak-beech forest with a important cover of brambles. Faeces samples were collected monthly from October 1987 to September 1988. The second area, inside the Nature reserve of "Les Hautes Fagnes" (50°30N, 6°06 E; 600 m) is an old peat-bog (Eriophorum vaginatum, Molinia coerulea, Vaccinium myrtillus and V. uliginosum) surrounded by spruce plantations. Sampling took place there only in April and in June 1989. Faeces were boiled and filtered before digestion in the Scharrer- Kurschner mixture for 30 min. Then samples were sieved under water and a small amount taken and mounted on a slide for microscopic observation. More than 13,000 small vegetal pieces were identified to the species by comparison between the observed epidermal features and a reference collection of plant epidermis. Woody plants are the main food of the roe deer in both areas: in the Hautes Fagnes, heather (Calluna vulgaris) and bilberry (Vaccinium) are the main food items whereas in Hotton, brambles are the most important part the diet in each season. In Hotton, we found that the diet was much more diversified during the green season than in the winter. The winter diet comprises mainly brambles but also heather an Vaccinium myrtillus in low quantities. Spruce needles are present only at the end of the winter. In the spring, grasses were taken but their contribution to the food of the deer was never important. During the summer, the roe deer ate more tree leaves (Quercus robur and Sorbus aucuparia). In the Hautes Fagnes, grasses were important only in the spring diet and tree leaves (Sorbus) in the summer. Some tree species were very common in both study areas (beech and spruce saplings) but nearly not eaten. The roe deer can thus considered as a selective feeder preferring high quality foods (spring grasses or young leaves) to bulky one (old grasses or hard leaves). [less ▲] Detailed reference viewed: 49 (1 ULg) Pour une production ligneuse de qualité : impératifs écologiques et sylvicoles.; ; Rondeux, Jacques ![]() Book published by Faculté des Sciences Agronomiques (1991) In highly industrialized countries the quality of wood production is becoming a concept that is gaining ground. Especially in temperates zones, according to the climate and the great variety of good soils ... [more ▼] In highly industrialized countries the quality of wood production is becoming a concept that is gaining ground. Especially in temperates zones, according to the climate and the great variety of good soils, healthy forests of high production can be created provided the choice of species takes site quality (vegetative characteristics, soil factors, site index) into account and the forest is intensively managed. Such a concept calls for an integrated approach based upon a careful knowledge of the forest ecosystem as a whole. [less ▲] Detailed reference viewed: 19 (1 ULg) La forêt et les forestiers : réalités, nouvelles approches et défisRondeux, Jacques ![]() Book published by Faculté des Sciences Agronomiques (1991) In this paper, the author states his views concerning the objective notion of « multiple use » forests and the forest ecosystem itself. The role of the forester and the challenges facing him in both ... [more ▼] In this paper, the author states his views concerning the objective notion of « multiple use » forests and the forest ecosystem itself. The role of the forester and the challenges facing him in both highly industrialized as well as developing countries are analyzed in the light of new approaches and concepts. Particular attention is focused on his role in the future and some of the points considered are : efforts to prevent depletion of natural resources, the integration of ecological, economic and sociological aspects in management practices, the decision making under risk situation. [less ▲] Detailed reference viewed: 12 (1 ULg) Application de l'échographie bidimentionnelle au diagnostic de gestation et à l'évaluation de l'incidence de la mortalité embryonnaire dans l'espèce bovine.Hanzen, Christian ; in Annales de Médecine Vétérinaire (1991), 135 Detailed reference viewed: 78 (4 ULg) Particularités physiologiques et physiopathologiques de la fonction pulmonaire des bovinsLekeux, Pierre ![]() in Point Vétérinaire (1991), 23(140), 793-799 This review détails the specific factors of respiratory function in cattle, the purpose being to understand cattle's great sensitivity te respiratory conditions, to be able to breed them for an optimal ... [more ▼] This review détails the specific factors of respiratory function in cattle, the purpose being to understand cattle's great sensitivity te respiratory conditions, to be able to breed them for an optimal functioning of their respiratory metabolism, and to adapt the prevention of respiratory conditions to the physiological specificity of this species. For each main respiratory syndrome, the consequences on the vital functions, and particularly for the respiratory function, are analyzed (9 tables, 33 references). [less ▲] Detailed reference viewed: 61 (5 ULg) Caractérisation des anomalies pluviométriques de la saison des pluies 1989-1990 dans le Sud-Est du Zaïre; ; Erpicum, Michel ![]() in Publications de l'Association Internationale de Climatologie (1991), 4 Detailed reference viewed: 11 (0 ULg) Conditional Heteroscedasticity in Stock Returns: International EvidenceCorhay, Albert ; E-print/Working paper (1991) Detailed reference viewed: 10 (3 ULg) |
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