Publications ORBi OA
Bookmark and Share    
Full Text
See detailFunctionalization of aliphatic polyesters by “click chemistry” in supercritical carbon dioxide
Grignard, Bruno ULg; Schmeits, Stephanie ULg; Riva, Raphaël ULg et al

Poster (2009, May 14)

The combination of ring-opening polymerization of lactones and “click” copper-catalyzed Huisgen’s [3+2] cycloaddition is known to be a very efficient strategy for the functionalization of poly(ε ... [more ▼]

The combination of ring-opening polymerization of lactones and “click” copper-catalyzed Huisgen’s [3+2] cycloaddition is known to be a very efficient strategy for the functionalization of poly(ε-caprolactone) (PCL) and poly(lactic acid) (PLA). Whenever the “click” reaction occurs in an organic solvent (THF or DMF), at relatively low temperature (35°C) and within short reaction time (2 hours), no significant degradation of polyester chains is detected. This strategy was implemented in previous works to graft alkynes substituted by different functional groups, such as hydroxyl, tertiary amines, acrylates or ammonium salts onto azide-functionalized PCL. Moreover, this approach was previously extended to the synthesis of grafted copolymers, either by the grafting of omega-alkyne-PEO onto azide-functionalized aliphatic PLA or PCL (“grafting onto” technique) either by grafting of an ATRP initiator followed by the polymerization of vinyl monomers, such as styrene (“grafting from” technique). These functionalized aliphatic polyesters are promising materials for the development of new biomedical devices. In this work, novel conditions were implemented for the “click” reaction in order to avoid the use of organic solvents and to limit the amount of catalyst remnants in functionalized aliphatic polyesters. Toward this end, if was found that the functionalization by “click” chemistry can be efficiently carried out in supercritical carbon dioxide rather than in THF or DMF. For that sake, it turned out necessary to synthesize a perfluorinated polyamine in order to solubilize the catalyst in supercritical carbon dioxide. Aliphatic polyesters are not soluble in supercritical carbon dioxide. Nevertheless, even under heterogeneous conditions, the functionalization of aliphatic polyesters by “click” chemistry is quantitative. Interestingly enough, no degradation was observed. Last but not least, the copper catalyst was easily removed by supercritical fluid extraction leading to a very low content of residual copper in the final copolyester. [less ▲]

Detailed reference viewed: 176 (18 ULg)
Full Text
See detailpH-sensitive micellar systems for controlled drug delivery: synthesis and structural characterization by small-angle neutron scattering
Joset, Arnaud ULg; Jérôme, Christine ULg; Brulet, Annie et al

Poster (2009, May 14)

The aim of the project is the preparation of micellar nanocarriers made of biocompatibles copolymers and their structural analysis by Small Angle Neutron Scattering (SANS). These micelles could be used in ... [more ▼]

The aim of the project is the preparation of micellar nanocarriers made of biocompatibles copolymers and their structural analysis by Small Angle Neutron Scattering (SANS). These micelles could be used in drug delivery applications to fight cancer1. The hydrophobic polycaprolactone (PCL) core is intended to incorporate the drug. The corona of hydrophilic polyethylene oxide (PEO) stabilizes the nanocarriers with respect to the plasma proteins. The pH in the neighborhood of the tumoral cells is lower than in the healthy cells. We incorporated a pH-sensitive sequence of poly(2-vinylpyridine) (P2VP). As a result, these micelles are expected to deliver their drug near the cancerous cells without affecting the healthy cells. When the pH is acidic, the P2VP is protonated and the chains are repulsive. The micellar size is then larger than in basic pH, when the P2VP is precipitated on the PCL core. We prepared PCL65-b-P2VP31 / PCL65-b-PEO114 and PCL32-b-P2VP52 / PCL36-b-PEO114 50:50 mixtures of diblocks copolymers. The resulting mixed micelles are analyzed by SANS. We developed a theoretical model with a spherical water-free PCL core. The PEO corona is described as consisting of gaussian chains with a thickness estimated as twice the chain gyration radius, Rg. We propose two alternatives for handling the P2VP zone. The P2VP molecules are either assumed to be Gaussian chains or they fill a shell of thickness L with possible water penetration.The fitting of the models to the experimental scattering cross sections leads to important structural parameters like the aggregation number, the core radius, the gyration radius and the thickness of the P2VP shell. [less ▲]

Detailed reference viewed: 91 (16 ULg)
Full Text
See detailMannosylated amphiphilic and degradable PEO-b-PCL copolymers for drug delivery systems: preparation and sugar availability characterizations
Freichels, Hélène ULg; Imberty, Anne; Auezély-Velty, Rachel et al

Poster (2009, May 14)

Over the last decade, polymer micelles and nanoparticles attracted an increasing interest in pharmaceutical research because they can be used as efficient drug delivery systems. In this field, amphiphilic ... [more ▼]

Over the last decade, polymer micelles and nanoparticles attracted an increasing interest in pharmaceutical research because they can be used as efficient drug delivery systems. In this field, amphiphilic copolymers combining poly(ethylene oxide) and aliphatic polyester (such as poly(ε-caprolactone) (PCL) or polylactide (PLA)) are particularly of interest because (i) PEO has unique protein- repellent properties and thus provides a stealth behaviour to the drug carriers and (ii) aliphatic polyesters are biocompatible and biodegradable hydrophobic matrices well-suited for the incorporation of an hydrophobic drug. By end-capping the hydrophilic segment by a targeting moiety so that they may interact with membrane receptors, the biodistribution of polymeric micelles and nanoparticles stabilized with this copolymer can be modulated and can induce specific cellular uptake by receptor-mediated endocytosis. One class of interesting targeting agent is the saccharides, in particular the mannose, because of its specific interaction with mannose receptor, which are found on peripheral and bone marrow macrophages, dendritic cells and sinusoidal liver cells. In this study, the reductive amination reaction is use to attach this targeting agent. After optimisation of the reaction with amino fluorescein, a model amine, mannosylated copolymer of PEO and PCL has been prepared. The surface availability of the saccharide upon the micelles in aqueous phosphate buffer was then assessed by DLS through binding with the protein Concanavalin A (ConA), a known mannose receptor. The interactions between the Bcla lectin and the mannosylated micelles have then been studied by Isothermal Titration Calorimetry (ITC) and the thermodynamic parameters have been obtained. This polymer is particularly useful for the stabilization of PLGA nanoparticles with the goal to target M cells for oral vaccination. [less ▲]

Detailed reference viewed: 105 (10 ULg)
Full Text
See detailDispersion nitroxide mediated polymerization of MMA in supercritical carbon dioxide
Grignard, Bruno ULg; Gigmes, Didier; Jérôme, Christine ULg et al

Poster (2009, May 14)

Controlled dispersion Nitroxide Mediated Polymerization (NMP) of methyl methacrylate (MMA) was successfully carried out for the first time in supercritical carbon dioxide (scCO2) in the presence of CO2 ... [more ▼]

Controlled dispersion Nitroxide Mediated Polymerization (NMP) of methyl methacrylate (MMA) was successfully carried out for the first time in supercritical carbon dioxide (scCO2) in the presence of CO2-philic perfluorinated surfactant that was generated “in situ”. The control of the MMA polymerization relies on the strategy developed by Charleux et al. that consists of using a SG1-based alkoxyamine, i.e. the block-builder, in the presence of small amount of styrene. In a first step, CO2 soluble polyheptadecafluorodecylacrylate was prepared in scCO2 using block-builder as an alkoxyamine. In a second step, nitroxide SG1 mediated dispersion polymerization of MMA was conducted at 70°C and 300 bar in the presence of 5 w% of SG1 terminated surfactant compared to the monomer. Different monomer to alkoxyamine molar ratios were investigated in order to target different molecular weights. In each case, the monomer conversion was high (>90 %), the experimental molecular weight was in good agreement with the theoretical value and the polydispersity was narrow (Mw/Mn ~1.2). Moreover, after depressurisation of the cell, PMMA was collected as a free flowing powder consisting of small sized microspheres. [less ▲]

Detailed reference viewed: 83 (9 ULg)
Full Text
See detailNovel Stealthy Gd(III)-DOTA/polymer Conjugates for Magnetic Resonance Imaging (MRI)
Grogna, Mathurin ULg; Bémelmans, Stéphanie ULg; Vanasschen, Christian ULg et al

Conference (2009, May 14)

Magnetic resonance imaging (MRI) is a routine diagnostic tool in modern clinical medicine. MRI has many advantages as a diagnostic imaging modality. It is noninvasive, delivers no radiation, and has ... [more ▼]

Magnetic resonance imaging (MRI) is a routine diagnostic tool in modern clinical medicine. MRI has many advantages as a diagnostic imaging modality. It is noninvasive, delivers no radiation, and has excellent (submillimeter) spatial resolution. Some Gadolinium(III) complexes are commonly used to enhance the contrast between adjacent tissues when the resolution/sensitivity of MRI is too low. Because free Gd3+ is very toxic in doses required for MRI, Gd(III) is chelated by poly(amino-carboxylate) such as diethylenetriamine pentaacetic acid (DTPA) or 1,4,7,10-Tetraazacyclododecane-1,4,7,10-tetraacetic acid (DOTA). Although DTPA/Gd3+ and DOTA/Gd3+ are water soluble, they have a very short circulation lifetime in blood, a low molecular weight and a short rotational time that make the contrast poor. To enhance the contrast, the Gd3+/complex doses have to be increased. In order to increase the sensitivity of the technique, while not increasing the concentration of the contrast agent, we were investigating different strategies to improve (i) the circulation lifetime in blood, (ii) the relaxation rate of Gd(III) (and consequently, the contrasting efficiency) and (iii) the targeting of the contrast agent. This presentation aims at reporting how a multifunctional (co)polymer can be designed and exploited for improving the contrasting ability and bioavailability of gadolinium-based complexes. [less ▲]

Detailed reference viewed: 99 (21 ULg)
Full Text
See detailMarcel Leclerq: Médecin, diptériste, parasitologue et pionnier de l'entomologie forensique
Mignon, Jacques ULg

Speech/Talk (2009)

Contribution biographique sur le pionnier en entomologie médico-légale qu'était Marcel Leclercq.

Detailed reference viewed: 50 (0 ULg)
Full Text
See detailLe Dossier Professionnel Réflexif (DPR) en didactique des Langues et littératures modernes à l'ULg : un outil pour développer l'esprit critique et l'autonomie des futurs enseignants
Simons, Germain ULg

Conference (2009, May 13)

Depuis l'année académique 2007-2008, le service de didactique des Langues et littératures modernes de l'ULg a introduit un Dossier Professionnel Réflexif (DPR) qui clôture la formation de l'AESS du Master ... [more ▼]

Depuis l'année académique 2007-2008, le service de didactique des Langues et littératures modernes de l'ULg a introduit un Dossier Professionnel Réflexif (DPR) qui clôture la formation de l'AESS du Master/à finalité didactique. Ce DPR, sorte de "portfolio", vise à amener les étudiants à prendre distance par rapport à leur formation, à s'auto-évaluer en tant que professeur-stagiaire, et à imaginer des pistes de solution afin de s'améliorer dans le futur. La présente communication décrit et commente le dispositif mis en place et fournit quelques éléments d'évaluation. [less ▲]

Detailed reference viewed: 178 (5 ULg)
Full Text
See detailDie belgisch-deutschen diplomatischen Beziehungen 1949-1991
Brüll, Christoph ULg

Speech/Talk (2009)

Detailed reference viewed: 62 (6 ULg)
Full Text
See detailExozodiacal discs with ALADDIN: how deep can we go?
Absil, Olivier ULg

Scientific conference (2009, May 12)

Studying the warm inner part of debris discs—-the extrasolar counterparts of the zodiacal dust cloud-—is of prime importance to characterise the global architecture of planetary systems. Furthermore, the ... [more ▼]

Studying the warm inner part of debris discs—-the extrasolar counterparts of the zodiacal dust cloud-—is of prime importance to characterise the global architecture of planetary systems. Furthermore, the possible presence of large quantities of warm dust around nearby main sequence stars is unanimously recognised as a major threat for future space missions dedicated to the direct detection and characterisation of Earth-like planets (either with visible/near-IR coronagraphy or mid-infrared interferometry). As of today, exozodiacal discs have been directly resolved around very few main sequence stars, at a sensitivity level of about 1000 times our zodiacal dust cloud. In this context, the ALADDIN project aims at detecting warm dust populations around nearby main sequence stars with significantly improved sensitivity. In this paper, we present a thorough study of ALADDIN's estimated performance. End-to-end simulations taking into account the specific characteristics of the Antarctic environment have been carried out, showing that a nulling interferometer coupled to a pair of 1-m class telescopes in Antarctica would perform significantly better than a similar instrument working on 8-m class telescopes in a temperate site. Exozodiacal dust density levels as low as 50 times the Solar zodiacal cloud are within reach around most Solar-type stars within 25 pc. Such performance would bring the study of exozodiacal light to a new level, and enable a fine study of terrestrial planet environments. Suitable candidate targets for direct Earth-like planet detection could then be identified among nearby main sequence stars in the Southern hemisphere, and the design of future space missions tuned to cope with the statistical occurrence of bright exozodiacal discs. [less ▲]

Detailed reference viewed: 11 (1 ULg)
Full Text
Peer Reviewed
See detailDetecting Triangle Inequality Violations in Internet Coordinate Systems by Supervised Learning
Liao, Yongjun ULg; Kaafar, Mohamed Ali; Gueye, Bamba et al

in Lecture Notes in Computer Science (2009, May 12), 5550

Internet Coordinates Systems (ICS) are used to predict Internet distances with limited measurements. However the precision of an ICS is degraded by the presence of Triangle Inequality Violations (TIVs ... [more ▼]

Internet Coordinates Systems (ICS) are used to predict Internet distances with limited measurements. However the precision of an ICS is degraded by the presence of Triangle Inequality Violations (TIVs). Simple methods have been proposed to detect TIVs, based e.g. on the empirical observation that a TIV is more likely when the distance is underestimated by the coordinates. In this paper, we apply supervised machine learning techniques to try and derive more powerful criteria to detect TIVs. We first show that (ensembles of) Decision Trees (DTs) learnt on our datasets are very good models for this problem. Moreover, our approach brings out a discriminative variable (called OREE), which combines the classical estimation error with the variance of the estimated distance. This variable alone is as good as an ensemble of DTs, and provides a much simpler criterion. If every node of the ICS sorts its neighbours according to OREE, we show that cutting these lists after a given number of neighbours, or when OREE crosses a given threshold value, achieves very good performance to detect TIVs. [less ▲]

Detailed reference viewed: 131 (32 ULg)
Full Text
See detailSupporting cell cytoskeleton during development of the organ of Corti in rat
Johnen, Nicolas ULg; Thelen, Nicolas ULg; Malgrange, Brigitte ULg et al

Poster (2009, May 11)

The mammalian auditory organ, the organ of Corti (OC), is composed of mechanosensory hair cells and nonsensory supporting cell types. Based on their morphology and physiology, a least four types of ... [more ▼]

The mammalian auditory organ, the organ of Corti (OC), is composed of mechanosensory hair cells and nonsensory supporting cell types. Based on their morphology and physiology, a least four types of supporting cells can be identified in the OC: inner pillar cell, outer pillar cell, phalangeal cell and Deiter’s cells. All supporting cells are highly specialized cells that are characterized by the presence of bundled microtubules with 15 protofilaments instead of 13. Using antibobies against different proteins of cytoskeleton (tubulin, cutokeratin and vimentin), we investigated by confocal microscopy the setting up of supporting cells' cytoskeleton during the differentiation of the OC in art from the embryonic day 18 (E18) to the postnatal 15 (P15). We showed that the inner pillar cells are labelled with an anti-beta IV tubulin from P0. Using an antibody to cytokeratin, a labelling appeared in Deiters' cells from E22. We also revealed that during the development of the OC, supporting cells were labelled with an anti-vimentin antibody from P0. [less ▲]

Detailed reference viewed: 36 (7 ULg)
Full Text
Peer Reviewed
See detailObserver l’écoute : du paradoxe à l’enquête
Colon, Paul-Louis ULg

Conference (2009, May 11)

La communication faisait un état critique des techniques d'observation de la perception auditive qui tiennent compte de sa dimension sociale et culturelle.

Detailed reference viewed: 47 (8 ULg)
Full Text
See detailHow much more Economics is the New Approach ?
Sibony, Anne-Lise ULg

Conference (2009, May 11)

Detailed reference viewed: 30 (3 ULg)
Full Text
Peer Reviewed
See detailMulti-Criteria Decision Support for Cost Assessment Techniques in Shipbuilding Industry
Caprace, Jean-David ULg; Rigo, Philippe ULg

in COMPIT'09, Budapest (2009, May 10)

Cost is perhaps the most influential factor in the outcome of a product or service within many of today’s industries. Cost assessment during the early stage of ship design is crucial. It influences the go ... [more ▼]

Cost is perhaps the most influential factor in the outcome of a product or service within many of today’s industries. Cost assessment during the early stage of ship design is crucial. It influences the go, no-go decision concerning a new development. Cost assessment occurs at various stages of ship design development. Economic evaluation as early as possible, in the design phase, is therefore crucial to find the best price–function compromise for the ship projects. This paper presents a Multi Criteria Decision Aid (MCDA) method in order to select an appropriate cost assessment method at each stage of the ship design. In order to compare the cost performance between all alternatives, the evaluation of each alternative is performed by PROMETHEE. [less ▲]

Detailed reference viewed: 120 (7 ULg)
Full Text
Peer Reviewed
See detailSpace Allocation Optimization Applied to Lower Hulls’ Production of Semi-Submersible Platforms
Caprace, Jean-David ULg; Marins, Cassiano; Losseau, Nicolas ULg et al

in COMPIT'09 (2009, May 10)

This paper presents a part of new developments included inside a holistic optimisation strategy during the design cycle of ships. The first part of the paper describes an algorithm to maximize the number ... [more ▼]

This paper presents a part of new developments included inside a holistic optimisation strategy during the design cycle of ships. The first part of the paper describes an algorithm to maximize the number of ship blocks and ship sections to produce in workshops during a certain time window. A tool was developed in order to support planners to improve the space utilization and workshop productivity. Thanks to this software, the scheduling is now done more efficiently and above all it takes less time. The second part of the paper describes a recent development of an algorithm able to generate a feasible blocks erection sequence starting from the blocks splitting definition. The idea is to integrate this module as a new functionality inside simulation software in order to take into account blocks sequence during the elaboration of the optimised production strategy. The two developments were validated on the lower hull of a semi-submersible platform coming from a new Brazilian shipyard. [less ▲]

Detailed reference viewed: 112 (14 ULg)
Full Text
See detailCoeur et Diabète. Objectifs de prévention cardiovasculaire
RADERMECKER, Régis ULg

Conference (2009, May 09)

Detailed reference viewed: 13 (1 ULg)