Publications ORBi OA
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See detailStability of recombinant 2 S albumin allergens in vitro
Murtagh, Gareth; Dumoulin, Mireille ULg; Alcocer, Marcos et al

in Biochemical Society Transactions (2002), 30

Two well known 2 S albumins, Ber e 1 from brazil nut and sunflower 2 S albumin 8 (SFA-8), have been expressed in a eukaryotic system and purified. Analysis of recombinant versions of Ber e 1 and SFA-8 ... [more ▼]

Two well known 2 S albumins, Ber e 1 from brazil nut and sunflower 2 S albumin 8 (SFA-8), have been expressed in a eukaryotic system and purified. Analysis of recombinant versions of Ber e 1 and SFA-8 revealed them to be significantly more resistant to digestion by pepsin than BSA, and to be stable for up to 30 min in simulated gastric fluid. Unfolding monitored by CD indicated that both proteins were also very resistant to denaturation induced by heat and low pH. These results suggest that, although the ability of 2 S albumins to reach the circulatory system may be a prerequisite for the allergenicity of this group of proteins, stability is just one of a number of characteristics that provoke a selective immune response. [less ▲]

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See detailLe mélèze, un bois de qualité aux multiples usages
Charron, Stéphane; Hebert, Jacques ULg; Jourez, Benoît ULg

in Forêt Wallonne (2002), (61), 37-41

Réputé pour son aspect esthétique, sa résistance mécanique et sa durabilité naturelle, le mélèze est une essence polyvalente, apte à de multiples usages tant intérieurs qu’extérieurs. Pourtant ... [more ▼]

Réputé pour son aspect esthétique, sa résistance mécanique et sa durabilité naturelle, le mélèze est une essence polyvalente, apte à de multiples usages tant intérieurs qu’extérieurs. Pourtant, l’industrie de la transformation belge ne s’y est pas beaucoup intéressée. <br />Plusieurs explications peuvent être évoquées : faibles volumes commercialisés, mauvaise réputation en raison de quelques expériences malheureuses, <br />etc. Actuellement, les préjugés s’estompent et la demande en bois de mélèze est croissante. [less ▲]

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See detailChoisir un scénario sylvicole pour les mélèzes
Pauwels, D.; Lejeune, Philippe ULg; Rondeux, Jacques ULg

in Forêt Wallonne (2002), (61), 17-24

Le choix du traitement sylvicole à appliquer à un peuplement forestier est une démarche qui peut s’avérer très complexe si l’on tient compte des délais entre la plantation et la récolte du peuplement ... [more ▼]

Le choix du traitement sylvicole à appliquer à un peuplement forestier est une démarche qui peut s’avérer très complexe si l’on tient compte des délais entre la plantation et la récolte du peuplement final, de l’évolution des filières de transformation, ou encore de la multiplicité des objectifs que peut poursuivre le gestionnaire forestier. [less ▲]

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See detailLa gestion forestière durable en Région wallonne, intégration d'indicateurs appropriés à l'inventaire permanent
Lecomte, H.; Koestel, G.; Rondeux, Jacques ULg

Book (2002)

The permanent forest inventory used in Wallonie since 1994 has been launched, as many others, to support a regional forest policy and evaluate wood forest products. To take into account criteria and ... [more ▼]

The permanent forest inventory used in Wallonie since 1994 has been launched, as many others, to support a regional forest policy and evaluate wood forest products. To take into account criteria and indicators of sustainable forest management, especially proposed by the Helsinki conference, some aspects of the methodology have been adapted and new variables have been collected in the field of ecological parameters (soil, lesser vegetation, ecosystem and species diversity, biomass, global carbon budget, forest health, physical environmental factors). This paper provides some insight into the way used to collect, through a regional permanent forest inventory, a large amount of components dealing with forest sustainability. An important database provides useful baseline information for fu-ture management decisions and can be considered as the best achieved tool for monitoring progress toward sustainable forest management at a regional level. [less ▲]

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See detailP2X(1)-mediated activation of extracellular signal-regulated kinase 2 contributes to platelet secretion and aggregation induced by collagen.
Oury, Cécile ULg; Toth-Zsamboki, Emese; Vermylen, Jos et al

in Blood (2002)

This study shows that mild platelet stimulation with collagen rapidly releases ATP, which activates the P2X(1)-PKC-ERK2 pathway. This process enhances further degranulation of the collagen-primed granules ... [more ▼]

This study shows that mild platelet stimulation with collagen rapidly releases ATP, which activates the P2X(1)-PKC-ERK2 pathway. This process enhances further degranulation of the collagen-primed granules allowing platelet aggregation to be completed. [less ▲]

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See detailDoes the P(2X1del) variant lacking 17 amino acids in its extracellular domain represent a relevant functional ion channel in platelets?
Oury, Cécile ULg; Toth-Zsamboki, Emese; Vermylen, Jos et al

in Blood (2002)

In this letter, we questionned the existence of a ADP responsive P2X1 protein variant in platelets.

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See detailFormadis : soutien au déploiement de formations ouvertes et à distance, en Wallonie et à Bruxelles
Dupont, Chantal ULg; Poumay, Marianne ULg; Georges, François ULg et al

(2002)

This paper presents an initiative, FORMADIS, aiming at producing a critical mass of quality courses and thus, contributing to the dissemination of good practices in ODL. A competitive call has been ... [more ▼]

This paper presents an initiative, FORMADIS, aiming at producing a critical mass of quality courses and thus, contributing to the dissemination of good practices in ODL. A competitive call has been launched in 2001 and 2002 to select 23 projects to be closely coached and turned into on-line active courses. The teachers, by working on their own course with the support of instructional, technical and graphic specialists move from a traditional way of considering their course to a more open view of what it could be. Applying the principles of isomorphism and transfer, the teacher training program in pedagogy, partly on line and partly face to face, helps in the different stages of development of the course. As anticipated, this setting appears to be an effective way to provide continuing education to teachers. The produced courses illustrate the diversity of implemented strategies and the added-value due to ODL. Over 40 activities have been described and analysed, according to the “7 teaching/learning paradigms” model. [less ▲]

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See detailPositive effects of glucocorticoids on T cell function by upregulation of interleukin-7 receptor alpha
Franchimont, Denis; Galon, Jérôme; Vacchio, Melanie S. et al

in Journal of Immunology (2002), 168

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See detailThymus, central T cell self-tolerance and type 1 diabetes
Geenen, Vincent ULg

in International Diabetes Monitor (2002), 14

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See detailThe Valence Quark Distribution of the Pion
Lansberg, Jean-Philippe ULg; Bissey, F.; Cudell, Jean-René ULg et al

in Dong Su; Burchat, Patricia (Eds.) XXII Physics in Collision Conference (PIC02) (2002)

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See detailOn the joint analysis of longitudinal responses and early discontinuation in randomized trials
Vandenhende, François; Lambert, Philippe ULg

in Journal of Biopharmaceutical Statistics (2002), 12

Our focus is on the joint analysis of longitudinal nonnormal responses and early discontinuation in (pre)-clinical trials. Separate models are fitted to the two series (response and discontinuation) to ... [more ▼]

Our focus is on the joint analysis of longitudinal nonnormal responses and early discontinuation in (pre)-clinical trials. Separate models are fitted to the two series (response and discontinuation) to account for covariate and time effects. The serial dependence and the dependence between response and drop-out are also modeled. This is done using particular dependence functions, called copulas. Copulas are used to create a joint distribution with given marginal distributions. Applications are given for the analysis of heart rate/morbidity in toxicology and pain severity/intake of rescue medications in a trial on migraine. Using copulas, the level of dependence between two variables remains invariant to changes in the marginal distribution of either variable. This proves interesting in modeling the association in a longitudinal setting when responses change over time. [less ▲]

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See detailA copula based model for multivariate non normal longitudinal data: analysis of a dose titration safety study on a new antidepressant
Lambert, Philippe ULg; Vandenhende, François

in Statistics in Medicine (2002), 21

A new model for multivariate non-normal longitudinal data is proposed. In a first step, each longitudinal series of data corresponding to a given response is modelled separately using a copula to relate ... [more ▼]

A new model for multivariate non-normal longitudinal data is proposed. In a first step, each longitudinal series of data corresponding to a given response is modelled separately using a copula to relate the marginal distributions of the response at each time of observation. In a second step, at each observation time, the conditional (on the past) distributions of each response are related using another copula describing the relationship between the corresponding variables. Note that there is no need to consider the same family of distributions for these response variables. The technique is illustrated in a dose titration safety study on a new antidepressant. The haemodynamic effect on diastolic blood pressure, systolic blood pressure and heart rate is studied. These three responses are measured repeatedly over time on ten healthy volunteers during the dose escalation. The available covariates are sex and the concentration of drug in the plasma at time of measurement. [less ▲]

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See detailA simple approach for the design of steel and composite sway building frames
Maquoi, René ULg; Jaspart, Jean-Pierre ULg

in Steel Structures (2002), 2(n°1), 1-11

The Merchant-Rankine approach is a practical design procedure which allows to predict the ultimate resistance of sway steel building frames with rigid structural joints. In the present paper the ... [more ▼]

The Merchant-Rankine approach is a practical design procedure which allows to predict the ultimate resistance of sway steel building frames with rigid structural joints. In the present paper the background and the traditional field of application of the Merchant-Rankine formula are first described. Its extension to the design of steel frames with so-called semi-rigid joints is then presented and justified. Finally its application to steel-concrete sway composite building frames is investigated and first conclusions of this work are drawn. [less ▲]

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See detailAttempt to enhance the suicide gene therapy agaisnt breast cancer cells by using connexin 43 gene
Grignet, Christine ULg; Hajitou, Amin; Noël, Agnès ULg et al

in Acta Clinica Belgica (2002), 57(2), 99

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See detailSpectators’ characteristics, expenses and feelings. Comparison between four top level sport events organized in the region of Liège
Lenzen, Benoît; François, Cédric; Jurion, Bernard ULg et al

in Laaksonen, K.; Löppönen, P.; Nykänen, E. (Eds.) et al Book of proceedings of the 10th congress of the European Association for Sport Management. Future of sport management (2002)

A team of researchers collected data from one thousand of spectators attending 10 sport events organized within the region of Liège during the year 1999. Spectators were randomly submitted to an ... [more ▼]

A team of researchers collected data from one thousand of spectators attending 10 sport events organized within the region of Liège during the year 1999. Spectators were randomly submitted to an individual interview. Questions were about four main themes: (1) socio-economic characteristics of spectators; (2) spending; (3) opinion about the sport event; and (4) opinion about the region. [less ▲]

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See detailSingle-domain antibody fragments with high conformational stability.
Dumoulin, Mireille ULg; Conrath, Katja; Van Meirhaeghe, Annemie et al

in Protein Science : A Publication of the Protein Society (2002), 11(3), 500-15

A variety of techniques, including high-pressure unfolding monitored by Fourier transform infrared spectroscopy, fluorescence, circular dichroism, and surface plasmon resonance spectroscopy, have been ... [more ▼]

A variety of techniques, including high-pressure unfolding monitored by Fourier transform infrared spectroscopy, fluorescence, circular dichroism, and surface plasmon resonance spectroscopy, have been used to investigate the equilibrium folding properties of six single-domain antigen binders derived from camelid heavy-chain antibodies with specificities for lysozymes, beta-lactamases, and a dye (RR6). Various denaturing conditions (guanidinium chloride, urea, temperature, and pressure) provided complementary and independent methods for characterizing the stability and unfolding properties of the antibody fragments. With all binders, complete recovery of the biological activity after renaturation demonstrates that chemical-induced unfolding is fully reversible. Furthermore, denaturation experiments followed by optical spectroscopic methods and affinity measurements indicate that the antibody fragments are unfolded cooperatively in a single transition. Thus, unfolding/refolding equilibrium proceeds via a simple two-state mechanism (N <--> U), where only the native and the denatured states are significantly populated. Thermally-induced denaturation, however, is not completely reversible, and the partial loss of binding capacity might be due, at least in part, to incorrect refolding of the long loops (CDRs), which are responsible for antigen recognition. Most interestingly, all the fragments are rather resistant to heat-induced denaturation (apparent T(m) = 60-80 degrees C), and display high conformational stabilities (DeltaG(H(2)O) = 30-60 kJ mole(-1)). Such high thermodynamic stability has never been reported for any functional conventional antibody fragment, even when engineered antigen binders are considered. Hence, the reduced size, improved solubility, and higher stability of the camelid heavy-chain antibody fragments are of special interest for biotechnological and medical applications. [less ▲]

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See detailQuintic deficient spline wavelets
Bastin, Françoise ULg; Laubin, Pascal

in Bulletin de la Société Royale des Sciences de Liège (2002), 71(3), 121-144

We show explicitely how to construct scaling functions and wavelets using quintic deficient splines with compact support and symmetry properties.

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See detailStudy of the role of ionogenic amino-acid residues in catalytic activity of thiamine triphosphatase from bovine kidney by means of chemical modification
Makarchikov, Alexander F; Luchko, A. A.; Bettendorff, Lucien ULg et al

in News of Biomedical Sciences (2002), 3

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See detailConsequences of t-channel unitarity for gamma(*) p and gamma(*) gamma(*) amplitudes
Cudell, Jean-René ULg; Martynov, E.; Soyez, G.

in Bentvelsen, S.; de Jong, P.; Koch, J. (Eds.) et al ICHEP 2002 (2002)

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