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See detailVaricella-Zoster Virus IE4 Protein Interacts with SR Proteins and Exports mRNAs through the TAP/NXF1 Pathway.
Ote, Isabelle ULg; Vandevenne, Patricia ULg; Bontems, Sébastien ULg et al

in PLoS ONE (2009), 4(11), 7882

Available data suggest that the Varicella-Zoster virus (VZV) IE4 protein acts as an important regulator on VZV and cellular genes expression and could exert its functions at post-transcriptional level ... [more ▼]

Available data suggest that the Varicella-Zoster virus (VZV) IE4 protein acts as an important regulator on VZV and cellular genes expression and could exert its functions at post-transcriptional level. However, the molecular mechanisms supported by this protein are not yet fully characterized. In the present study, we have attempted to clarify this IE4-mediated gene regulation and identify some cellular partners of IE4. By yeast two-hybrid and immunoprecipitation analysis, we showed that IE4 interacts with three shuttling SR proteins, namely ASF/SF2, 9G8 and SRp20. We positioned the binding domain in the IE4 RbRc region and we showed that these interactions are not bridged by RNA. We demonstrated also that IE4 strongly interacts with the main SR protein kinase, SRPK1, and is phosphorylated in in vitro kinase assay on residue Ser-136 contained in the Rb domain. By Northwestern analysis, we showed that IE4 is able to bind RNA through its arginine-rich region and in immunoprecipitation experiments the presence of RNA stabilizes complexes containing IE4 and the cellular export factors TAP/NXF1 and Aly/REF since the interactions are RNase-sensitive. Finally, we determined that IE4 influences the export of reporter mRNAs and clearly showed, by TAP/NXF1 knockdown, that VZV infection requires the TAP/NXF1 export pathway to express some viral transcripts. We thus highlighted a new example of viral mRNA export factor and proposed a model of IE4-mediated viral mRNAs export. [less ▲]

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See detailAlignement et optimisation d'un simulateur solaire d'une configuration de 1SC à une configuration à 10SC.
De Rauw, Dominique ULg; Mazy, Emmanuel ULg; Stockman, Yvan ULg et al

Conference (2009, November 18)

Ce papier décrit les tâches réalisées dans le cadre d’un projet de modification du simulateur solaire de l’ESA afin de l’adapter et le faire passer d’une configuration où le flux est de une constante ... [more ▼]

Ce papier décrit les tâches réalisées dans le cadre d’un projet de modification du simulateur solaire de l’ESA afin de l’adapter et le faire passer d’une configuration où le flux est de une constante solaire vers une configuration à 10 constantes solaires. Nous exposons les tâches réalisées par le Centre Spatial de Liège en ce qui concerne la modélisation et la simulation des configurations ainsi que les éléments mis au point pour l’alignement des miroirs du simulateur solaire et pour la mesure d’uniformité de flux. [less ▲]

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See detailInfluence of geometry in the case of relative positioning with GNSS
Wautelet, Gilles ULg; Lejeune, Sandrine; Lonchay, Matthieu ULg et al

Poster (2009, November 18)

Relative positioning with GNSS is generally used to achieve precise positions in the frame of critical applications (surveying, photo-control...). On this basis, we have developed a software which allows ... [more ▼]

Relative positioning with GNSS is generally used to achieve precise positions in the frame of critical applications (surveying, photo-control...). On this basis, we have developed a software which allows to compute a positioning error due to the ionosphere only using reference stations belonging to the Belgian Dense Network (BDN). This network consists in 66 GPS (dual-frequency) receivers over the whole Belgium. The drawback of this method is that this computation needs the design matrix which contains coefficients depending on satellite constellation geometry. Therefore, like for absolute positioning, a poor geometry (evaluated by the Dilution of Precision, or DOP) can also lead to large positioning error that cannot be separated from the one due to ionospheric effects, and in particular the small-scale structures. The main goal of this paper is to build a similar index to DOP for relative positioning in our software to be able to separate the ionospheric effects from the geometric ones. The final step is to study the feasability of a service for users of relative positioning using the BDN. The objective is to give in post-processing the positioning accuracy degradation for all BDN baselines and to associate a colour scheme to the different degradation classes created. [less ▲]

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See detailLe traitement du cancer
RONDIA, Karine; VANDER STEICHEL, Didier; Barthelemy, Nicole ULg et al

Article for general public (2009)

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See detailA new look to an old measurement.
Pierard, Luc ULg; Magne, Julien ULg

in Journal of the American College of Cardiology (2009), 54(21), 1969-70

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See detailMise au point d’un protocole de micropropagation in vitro de Jatropha curcas L
Medza Mve, Samson Daudet ULg; Mergeai, Guy ULg; Baudoin, Jean-Pierre ULg et al

in Biotechnologie végétales: amélioration des plantes et sécurité alimentaire» et par le réseau « Biotechnologies Végétales et Biosécurité » du projet SISTBiotechnologies et valorisation du patrimoine végétal sous-exploité en zone sahélienne et soudano-sahélienne (2009, November 14)

Jatropha curcas L. est considéré comme une source potentielle non-comestible pour la production de biocarburants (Sujatha et al, 2005). Sa multiplication par semis présente l’inconvénient de cultiver au ... [more ▼]

Jatropha curcas L. est considéré comme une source potentielle non-comestible pour la production de biocarburants (Sujatha et al, 2005). Sa multiplication par semis présente l’inconvénient de cultiver au champ des plantes hétérogènes, dont les graines présentent des contenus en huile qui varie de 4-40% (Heller, 1996). La présente étude vise la mise au point d’un protocole de multiplication massale de plantes élites à partir d’explants nodaux cultivés en conditions axéniques. [less ▲]

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See detailVehicle loading optimization with stochastic supply
Pironet, Thierry ULg

Scientific conference (2009, November 13)

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See detailThe dashundergaps package
Merciadri, Luca ULg

Computer development (2009)

This is the manual of the dashundergaps package that I wrote. Briefly, this package aims at helping the LaTeX user at underlining a given set of words by either a line (using \underline{text}), dots ... [more ▼]

This is the manual of the dashundergaps package that I wrote. Briefly, this package aims at helping the LaTeX user at underlining a given set of words by either a line (using \underline{text}), dots (using \dotuline{text}) or even a dashed line (using \dashuline{text}). Various options were implemented for this. This can be used in conjunction with the phantomtext option, which allows the user to write a text with gaps, e.g. for a completion by students. These gaps can be completed with the real input using the teachernotes option. Please note that this is a really basic package. For more info on it, please look at the manual in the tarball. [less ▲]

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See detailMultidentate small-molecule inhibitors of vaccinia H1-related (VHR) phosphatase decrease proliferation of cervix cancer cells.
Wu, Shuangding; Vossius, Sofie ULg; Rahmouni, Souad ULg et al

in Journal of Medicinal Chemistry (2009), 52(21), 6716-23

Loss of VHR phosphatase causes cell cycle arrest in HeLa carcinoma cells, suggesting that VHR inhibition may be a useful approach to halt the growth of cancer cells. We recently reported that VHR is ... [more ▼]

Loss of VHR phosphatase causes cell cycle arrest in HeLa carcinoma cells, suggesting that VHR inhibition may be a useful approach to halt the growth of cancer cells. We recently reported that VHR is upregulated in several cervix cancer cell lines as well as in carcinomas of the uterine cervix. Here we report the development of multidentate small-molecule inhibitors of VHR that inhibit its enzymatic activity at nanomolar concentrations and exhibit antiproliferative effects on cervix cancer cells. Chemical library screening was used to identify hit compounds, which were further prioritized in profiling and kinetic experiments. SAR analysis was applied in the search for analogs with improved potency and selectivity, resulting in the discovery of novel inhibitors that are able to interact with both the phosphate-binding pocket and several distinct hydrophobic regions within VHR’s active site. This multidentate binding mode was confirmed by X-ray crystallography. The inhibitors decreased the proliferation of cervix cancer cells, while growth of primary normal keratinocytes was not affected. These compounds may be a starting point to develop drugs for the treatment of cervical cancer. [less ▲]

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See detailSensitivity analysis on the elastic buckling and ultimate strength of continuous stiffened aluminium plates under combined in-plane compression and lateral pressure
Khedmati, Mohammad Reza; Zareei, Mohammad Reza; Rigo, Philippe ULg

in Thin-Walled Structures (2009), 47

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See detailAbusive margin squeeze: an overview of European national case laws
Petit, Nicolas ULg; Provost, Elise ULg

in e-Competitions (2009)

The purpose of this brief article is to introduce the reader to the national case-law on abusive margin squeezes that was commented in the e-competitions bulletin over the period 2003-2009.To this end, it ... [more ▼]

The purpose of this brief article is to introduce the reader to the national case-law on abusive margin squeezes that was commented in the e-competitions bulletin over the period 2003-2009.To this end, it seeks, to the extent possible, to analyze the 23 e-competitions case notes in relation to six items which are generally reviewed in margin squeeze cases, i.e., existence of an upstream input and a downstream product/service (1); indispensability of the upstream input (2); dominance in the upstream market and vertical integration (3); particularly aggressive downstream pricing policy (4); anticompetitive effects (5); absence of objective justifications (6). In addition, the present article reviews a seventh issue, namely sanctions and remedies (7), where national enforcement practices may again diverge/converge. [less ▲]

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See detailKu proteins interact with activator protein-2 transcription factors and contribute to ERBB2 overexpression in breast cancer cell lines.
Nolens, Grégory ULg; Pignon, Jean-Christophe ULg; Koopmansch, Benjamin ULg et al

in Breast Cancer Research [=BCR] (2009), 11(6),

INTRODUCTION: Activator protein-2 (AP-2) alpha and AP-2 gamma transcription factors contribute to ERBB2 gene overexpression in breast cancer. In order to understand the mechanism by which the ERBB2 gene ... [more ▼]

INTRODUCTION: Activator protein-2 (AP-2) alpha and AP-2 gamma transcription factors contribute to ERBB2 gene overexpression in breast cancer. In order to understand the mechanism by which the ERBB2 gene is overexpressed we searched for novel AP-2 interacting factors that contribute to its activity. METHODS: Ku proteins were identified as AP-2 alpha interacting proteins by glutathione serine transferase (GST)-pull down followed by mass spectrometry. Transfection of the cells with siRNA, expression vectors and reporter vectors as well as chromatin immunoprecipitation (ChIP) assay were used to ascertain the implication of Ku proteins on ERBB2 expression. RESULTS: Nuclear proteins from BT-474 cells overexpressing AP-2 alpha and AP-2 gamma were incubated with GST-AP2 or GST coated beads. Among the proteins retained specifically on GST-AP2 coated beads Ku70 and Ku80 proteins were identified by mass spectrometry. The contribution of Ku proteins to ERBB2 gene expression in BT-474 and SKBR3 cell lines was investigated by downregulating Ku proteins through the use of specific siRNAs. Depletion of Ku proteins led to downregulation of ERBB2 mRNA and protein levels. Furthermore, reduction of Ku80 in HCT116 cell line decreased the AP-2 alpha activity on a reporter vector containing an AP-2 binding site linked to the ERBB2 core promoter, and transfection of Ku80 increased the activity of AP-2 alpha on this promoter. Ku siRNAs also inhibited the activity of this reporter vector in BT-474 and SKBR3 cell lines and the activity of the ERBB2 promoter was further reduced by combining Ku siRNAs with AP-2 alpha and AP-2 gamma siRNAs. ChIP experiments with chromatin extracted from wild type or AP-2 alpha and AP-2 gamma or Ku70 siRNA transfected BT-474 cells demonstrated Ku70 recruitment to the ERBB2 proximal promoter in association with AP-2 alpha and AP-2 gamma. Moreover, Ku70 siRNA like AP-2 siRNAs, greatly reduced PolII recruitment to the ERBB2 proximal promoter. CONCLUSIONS: Ku proteins in interaction with AP-2 (alpha and gamma) contribute to increased ERBB2 mRNA and protein levels in breast cancer cells. [less ▲]

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See detailActivités de l'unité de Chimie Biologique Industrielle
Jacquet, Nicolas ULg; Paquot, Michel ULg

Speech/Talk (2009)

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See detailEtude chromatographique de substances volatiles: cas des sémiochimiques
Heuskin, Stéphanie ULg

Scientific conference (2009, November 10)

Detailed reference viewed: 45 (15 ULg)