Compte rendu de « Robert SACRÉ, Les Negro Spirituals et les Gospel Songs, Paris, P.U.F. (Que sais-je ? 2791), 1993 »

in Bulletin de la Société liégeoise de Musicologie (1994), 86

Short-circuit mechanical effect on bundled conductors- IEC 865 recommendations : confrontations with tests and numerical computations.

in Proceedings of the 6th ESCC (1994, September)

IEC 865 discussion with confrontations between tests and numerical computations.

Polymorphism, an extension of binary opening

in Second international conference on Mathematical Morphology and its Applications to Image Processing, Proceedings (1994, September)

L'amélioration de l'environnement nutritionnel à l'école

in Andrien, Michel (Ed.) Actes du colloque européen sur l'éducation nutritionnelle dans le cadre d'une école promotrice de santé (1994, September)

Human xeroderman group G gene encodes a DNA endonuclease

in Nucleic Acids Research (1994), 22(16), 3312-6

The 6-Kilobase C-Erbb2 Promoter Contains Positive and Negative Regulatory Elements Functional in Human Mammary Cell Lines

in Cancer Research (1994), 54(15), 4193-9

A 6-kilobase fragment extending at the 5'-end of the c-erbB2 protooncogene was isolated from a normal human lymphocyte genomic DNA library. The full-length fragment and five subfragments with identical 3 ... [more ▼]

A 6-kilobase fragment extending at the 5'-end of the c-erbB2 protooncogene was isolated from a normal human lymphocyte genomic DNA library. The full-length fragment and five subfragments with identical 3'-ends were obtained by progressive unidirectional deletion from the 5'-end and were cloned in front of the luciferase reporter gene. The hybrid genes were analyzed for transcriptional activity in human mammary cell lines synthesizing low (HBL-100 and T-47D), moderate (MDA-MB-453), or high (BT-474) amounts of the c-erbB2 mRNA and were also analyzed in HeLa cells. Gene-specific expression was observed, indicating the presence of multiple cis-acting sequences in the c-erbB2 promoter. A major negative element is located in the -2- to -4-kilobase region. It is flanked on both sides by positive elements that display enhanced transcriptional activity in the BT-474 tumor cells only. While predominant in the low-expressing cells, the effect of the repressor appears to be overcome by the distal transactivator in the high-expressing BT-474 cells, resulting in a 15 to 50 times increase in luciferase activity relative to the HBL-100 and T-47D cells, respectively. Cell-specific expression relies on the trans-acting factors present in the different cell lines. The formation of cell-specific protein-DNA complexes was demonstrated by gel retardation assay. [less ▲]

Epidemiology of the Insulin Resistance Syndrome in Belgian Adults - Monica Bellux

Conference (1994, July 06)

Cold Adaptation of Proteins. Purification, Characterization, and Sequence of the Heat-Labile Subtilisin from the Antarctic Psychrophile Bacillus Ta41

in Journal of Biological Chemistry (1994), 269(26), 17448-53

The gene of subtilisin S41, an alkaline protease secreted by the psychrophile Bacillus TA41, encodes for a preproenzyme of 419 amino acids residues. The nucleotide sequence and NH2- and COOH-terminal ... [more ▼]

The gene of subtilisin S41, an alkaline protease secreted by the psychrophile Bacillus TA41, encodes for a preproenzyme of 419 amino acids residues. The nucleotide sequence and NH2- and COOH-terminal amino acid sequencing of the purified enzyme indicate that the mature subtilisin S41 is composed of 309 residues with a predicted M(r) = 31,224. Subtilisin S41 shares most of its properties with mesophilic subtilisins (structure of the precursor, 52% amino acid sequence identity, alkaline pH optimum, broad specificity, Ca2+ binding) but is characterized by a higher specific activity on macromolecular substrate, by a shift of the optimum of activity toward low temperatures, and by a low thermal stability. The enzyme also differs by an acidic pI (5.3) and the presence of one disulfide bond. It is proposed that the psychrophilic enzyme possesses a more flexible molecular structure when compared to mesophilic and thermophilic subtilases in order to compensate for the reduction of reaction rates at low temperatures. The model of subtilisin S41 indeed reveals several features able to induce a more flexible, heat-labile conformation: the occurrence of four extended surface loops, a very hydrophilic surface through 11 extra Asp residues, and the lack of several salt bridges and aromatic-aromatic interactions. The low affinity of the Ca1 calcium binding site (Kd(app) = 10(-6) M), resulting possibly from one chelating side chain substitution and the stacking of Gly residues, also reflect a less compact conformation. The difference of free energy of stabilization between subtilisin S41 and a mesophilic subtilisin suggests that the balance of exo- and endothermically formed weak bonds is critical for the enzyme flexibility. [less ▲]

Comment je traite un patient hypertendu âgé

in Revue Médicale de Liège (1994), 49(6), 303-304

La stratégie thérapeutique proposée en pratique est la suivante. 1. Le traitement de personnes âgées hypertendues doit être initié à partir du moment où les valeurs de pression artérielle ont été mesurées ... [more ▼]

La stratégie thérapeutique proposée en pratique est la suivante. 1. Le traitement de personnes âgées hypertendues doit être initié à partir du moment où les valeurs de pression artérielle ont été mesurées à plusieurs reprises et trouvées dépassant les valeurs de 160et/ou 95 mmHg, en raison de la démonstration de l'efficacité certaine de ce traitement dans la diminution de l'incidence des complications cardiovasculaires du patient hypertendu âgé. 2. Le régime sans sel strict doit être évité. Une correction de l'excès de poids doit bien sûr être associée au traitement médicamenteux. 3. Pour le traitement médicamenteux, il est nécessaire de choisir des agents antihypertenseurs dont l'utilisation est bien connue du prescripteur. 4. Ne pas viser d'emblée l'efficacité maximale. Il est conseillé d'augmenter la posologie par palliers successifs. 5. Une surveillance attentive du patient est nécessaire avec recherche des effets secondaires médicamenteux. 6. Si le traitement est inefficace, il est nécessaire de remettre en question ce premier choix. Celui-ci est classiquement un diurétique ou, en cas de contre-indication, un antagoniste calcique. 7. Le schéma thérapeutique doit être facile à comprendre du patient et consister en une prise unique. 8. Rappelons que le but du traitement est de prolonger l'existence du patient tout en assurant une qualité de vie correcte. [less ▲]

A Group Communication Framework

in Bauerfeld, Wulf; Spaniol, Otto; Williams, F. (Eds.) Broadband Islands '94: Connecting with the End-User (1994, June)

This paper presents a general architectural model for group communications which are communications that may involve more than two parties. The concept of group is presented and the concept of group ... [more ▼]

This paper presents a general architectural model for group communications which are communications that may involve more than two parties. The concept of group is presented and the concept of group association, which is an instance of group communication, is analysed. Those group associations are modeled as a set of basic components called multicast conversations. At the service boundery, new architectural concepts are introduced to identify a group association as well as its multicast conversations. Then, facilities to handle the group associations and the multicast conversations are defined and the properties of data transfer on a group association are examined. Finally, the paper deals with aspects of group management. [less ▲]