Publications ORBi OA
Genetic variation in the familial Mediterranean fever gene (MEFV) and risk for Crohn's disease and ulcerative colitis.
; ; Louis, Edouard et al
in PLoS ONE (2009), 4(9), 7154
BACKGROUND AND AIMS: The familial Mediterranean fever (FMF) gene (MEFV) encodes pyrin, a major regulator of the inflammasome platform controlling caspase-1 activation and IL-1beta processing. Pyrin has ... [more ▼]
BACKGROUND AND AIMS: The familial Mediterranean fever (FMF) gene (MEFV) encodes pyrin, a major regulator of the inflammasome platform controlling caspase-1 activation and IL-1beta processing. Pyrin has been shown to interact with the gene product of NLRP3, NALP3/cryopyrin, also an important active member of the inflammasome. The NLRP3 region was recently reported to be associated with Crohn's disease (CD) susceptibility. We therefore sought to evaluate MEFV as an inflammatory bowel disease (IBD) susceptibility gene. METHODOLOGY AND RESULTS: MEFV colonic mucosal gene expression was significantly increased in experimental colitis mice models (TNBS p<0.0003; DSS p<0.006), in biopsies from CD (p<0.02) and severe ulcerative colitis (UC) patients (p<0.008). Comprehensive genetic screening of the MEFV region in the Belgian exploratory sample set (440 CD trios, 137 UC trios, 239 CD cases, 96 UC cases, and 107 healthy controls) identified SNPs located in the MEFV 5' haplotype block that were significantly associated with UC (rs224217; p = 0.003; A allele frequency: 56% cases, 45% controls), while no CD associations were observed. Sequencing and subsequent genotyping of variants located in this associated haplotype block identified three synonymous variants (D102D/rs224225, G138G/rs224224, A165A/rs224223) and one non-synonymous variant (R202Q/rs224222) located in MEFV exon 2 that were significantly associated with UC (rs224222: p = 0.0005; A allele frequency: 32% in cases, 23% in controls). No consistent associations were observed in additional Canadian (256 CD trios, 91 UC trios) and Scottish (495 UC, 370 controls) sample sets. We note that rs224222 showed marginal association (p = 0.012; G allele frequency: 82% in cases, 70% in controls) in the Canadian sample, but with a different risk allele. None of the NLRP3 common variants were associated with UC in the Belgian-Canadian UC samples and no significant interactions were observed between NLRP3 and MEFV that could explain the observed flip-flop of the rs224222 risk allele. CONCLUSION: The differences in association levels observed between the sample sets may be a consequence of distinct founder effects or of the relative small sample size of the cohorts evaluated in this study. However, the results suggest that common variants in the MEFV region do not contribute to CD and UC susceptibility. [less ▲]Detailed reference viewed: 71 (14 ULg)
Développement d'embryons zygotiques hybrides entre un cultivar (NI637) de Phaseolus vulgaris L. et un génotype sauvage (NI108) de P. coccineus L.
; Baudoin, Jean-Pierre ; et al
in International Journal of Biology and Chemical Sciences (2009), 3(1),Detailed reference viewed: 26 (5 ULg)
Analyse dynamique du phénomène d'auto-blocage dans les charnières de type Carpentier
Hoffait, Sébastien ; Bruls, Olivier ; et al
in Colloque National en Calcul des Structures, Giens, 2009 (2009)Detailed reference viewed: 47 (9 ULg)
Common variants in the NLRP3 region contribute to Crohn's disease susceptibility.
; ; et al
in Nature Genetics (2009), 41(1), 71-6
We used a candidate gene approach to identify a set of SNPs, located in a predicted regulatory region on chromosome 1q44 downstream of NLRP3 (previously known as CIAS1 and NALP3) that are associated with ... [more ▼]
We used a candidate gene approach to identify a set of SNPs, located in a predicted regulatory region on chromosome 1q44 downstream of NLRP3 (previously known as CIAS1 and NALP3) that are associated with Crohn's disease. The associations were consistently replicated in four sample sets from individuals of European descent. In the combined analysis of all samples (710 father-mother-child trios, 239 cases and 107 controls), these SNPs were strongly associated with risk of Crohn's disease (P(combined) = 3.49 x 10(-9), odds ratio = 1.78, confidence interval = 1.47-2.16 for rs10733113), reaching a level consistent with the stringent significance thresholds imposed by whole-genome association studies. In addition, we observed significant associations between SNPs in the associated regions and NLRP3 expression and IL-1beta production. Mutations in NLRP3 are known to be responsible for three rare autoinflammatory disorders. These results suggest that the NLRP3 region is also implicated in the susceptibility of more common inflammatory diseases such as Crohn's disease. [less ▲]Detailed reference viewed: 105 (20 ULg)
Dyspeptic symptoms in the general population: a factor and cluster analysis of symptom groupings.
; ; Louis, Edouard et al
in Neurogastroenterology & Motility : The Official Journal of the European Gastrointestinal Motility Society (2009), 21(4), 378-88
Both dyspeptic and gastro-oesophageal reflux-like symptoms are frequent in the general population, but their degree of overlap is unknown. In severe functional dyspepsia (FD), symptoms are organized in ... [more ▼]
Both dyspeptic and gastro-oesophageal reflux-like symptoms are frequent in the general population, but their degree of overlap is unknown. In severe functional dyspepsia (FD), symptoms are organized in factors associated with pathophysiological mechanisms. The aims of this study were: (i) to assess the prevalence of dyspeptic symptoms with and without overlapping reflux symptoms in the general population and their impact on daily life and on healthcare utilization; and (ii) to compare symptom groupings in the general population to FD patients. A total of 2025 subjects, representative of the Belgian general population, were used in this study. The subjects were submitted to a questionnaire with validated questions on their dyspeptic and reflux symptoms and with evaluators of impact on daily life and use of healthcare resources. Significant dyspeptic symptoms were found in 417 (20.6%). Overlapping reflux symptoms were present in 141 (33.8%). In this group, symptoms were more frequent and more severe. Dyspeptic symptoms induced weight loss (12.7%) and absenteeism (12.4%), affected daily life (61.2%) and generated use of healthcare resources, such as medical consultations (61.4%) and medication (70.9%). Factor analysis revealed a three-component structure with factor 1 including fullness, bloating and early satiety, factor 2 including nausea and vomiting and factor 3 including discomfort, pain, belching and reflux. If forced in a four-factor model, the analysis separates belching as independent factor. Dyspeptic symptoms are frequent in the general population, with overlapping reflux symptoms and increased symptom burden in about a third. [less ▲]Detailed reference viewed: 54 (3 ULg)
Dossier de progression et dossier de réussite : deux outils complémentaires au service de la professionnalisation enseignante dans le supérieur
Poumay, Marianne ; Georges, François
in Collected Essays on Learning and Teaching (2009), II
Cet article aborde et illustre les notions de référentiel de compétences, de dossier de progression et de dossier de réussite (Tardif, 2006) tels qu’ils sont mis en oeuvre au sein du programme Formasup ... [more ▼]
Cet article aborde et illustre les notions de référentiel de compétences, de dossier de progression et de dossier de réussite (Tardif, 2006) tels qu’ils sont mis en oeuvre au sein du programme Formasup, master complémentaire en pédagogie de l’enseignement supérieur organisé à l’université de Liège. Les auteurs présentent l’articulation interne de ce programme qui cible un nombre limité de compétences critiques, accompagne le développement de ces compétences chez les enseignants participants et mesure ce développement à travers un portfolio. Ils discutent enfin des défis et difficultés qui persistent ainsi que des moyens mis en place par l’équipe encadrante pour tenter de les surmonter. [less ▲]Detailed reference viewed: 203 (43 ULg)
Insights into bacterial cellulose biosynthesis by functional metagenomics on Antarctic soil samples.
Berlemont, Renaud ; Delsaute, Maud ; Pipers, Delphine et al
in ISME Journal (The) (2009), 3(9), 1070-1081
In this study, the mining of an Antarctic soil sample by functional metagenomics allowed the isolation of a cold-adapted protein (RBcel1) that hydrolyzes only carboxymethyl cellulose. The new enzyme is ... [more ▼]
In this study, the mining of an Antarctic soil sample by functional metagenomics allowed the isolation of a cold-adapted protein (RBcel1) that hydrolyzes only carboxymethyl cellulose. The new enzyme is related to family 5 of the glycosyl hydrolase (GH5) protein from Pseudomonas stutzeri (Pst_2494) and does not possess a carbohydrate-binding domain. The protein was produced and purified to homogeneity. RBcel1 displayed an endoglucanase activity, producing cellobiose and cellotriose, using carboxymethyl cellulose as a substrate. Moreover, the study of pH and the thermal dependence of the hydrolytic activity shows that RBcel1 was active from pH 6 to pH 9 and remained significantly active when temperature decreased (18% of activity at 10 degrees C). It is interesting that RBcel1 was able to synthetize non-reticulated cellulose using cellobiose as a substrate. Moreover, by a combination of bioinformatics and enzyme analysis, the physiological relevance of the RBcel1 protein and its mesophilic homologous Pst_2494 protein from P. stutzeri, A1501, was established as the key enzymes involved in the production of cellulose by bacteria. In addition, RBcel1 and Pst_2494 are the two primary enzymes belonging to the GH5 family involved in this process.The ISME Journal advance online publication, 21 May 2009; doi:10.1038/ismej.2009.48. [less ▲]Detailed reference viewed: 102 (38 ULg)
Finite-Element Analysis of a Shielded Pulsed-Current Induction Heater-- Experimental Validation of a Time-Domain Thin-Shell Approach
V Sabariego, Ruth ; ; et al
in Proceedings of the 8th International Symposium on Electric and Magnetic Fields (EMF2009) (2009)
A time-domain extension of the classical frequency-domain thin-shell approach is used for the finite-element analysis of a shielded pulse-current induction heater. The time-domain interface conditions at ... [more ▼]
A time-domain extension of the classical frequency-domain thin-shell approach is used for the finite-element analysis of a shielded pulse-current induction heater. The time-domain interface conditions at the shell surface are expressed in terms of the average (zero-order) instantaneous flux and current density vectors in the shell, as well as in terms of a limited number of higher-order components. The three-dimensional thin-shell model is validated by comparing the numerical results with measurements performed on the heating device at different working frequencies. [less ▲]Detailed reference viewed: 44 (7 ULg)
Modeling milk urea of Walloon dairy cows in management perspectives.
Bastin, Catherine ; ; Gillon, Alain et al
in Journal of Dairy Science (2009), 92(7), 3529-40
The aim of this study was to develop an adapted random regression test-day model for milk urea (MU) and to study the possibility of using predictions and solutions given by the model for management ... [more ▼]
The aim of this study was to develop an adapted random regression test-day model for milk urea (MU) and to study the possibility of using predictions and solutions given by the model for management purposes. Data included 607,416 MU test-day records of first-lactation cows from 632 dairy herds in the Walloon Region of Belgium. Several advanced features were used. First, to detect the herd influence, the classical herd x test-day effect was split into 3 new effects: a fixed herd x year effect, a fixed herd x month-period effect, and a random herd test-day effect. A fixed time period regression was added in the model to take into account the yearly oscillations of MU on a population scale. Moreover, first autoregressive processes were introduced and allowed us to consider the link between successive test-day records. The variance component estimation indicated that large variance was associated with the random herd x test-day effect (48% of the total variance), suggesting the strong influence of herd management on the MU level. The heritability estimate was 0.13. By comparing observed and predicted MU levels at both the individual and herd levels, target ranges for MU concentrations were defined to take into account features of each cow and each herd. At the cow level, an MU record was considered as deviant if it was <200 or >400 mg/L (target range used in the field) and if the prediction error was >50 mg/L (indicating a significant deviation from the expected level). Approximately 7.5% of the MU records collected between June 2007 and May 2008 were beyond these thresholds. This combination allowed for the detection of potentially suspicious cows. At the herd level, the expected MU level was considered as the sum of the solutions for specific herd effects. A herd was considered as deviant from its target range when the prediction error was greater than the standard deviation of MU averaged by herd test day. Results showed that 6.7% of the herd test-day MU levels between June 2007 and May 2008 were considered deviant. These deviations seemed to occur more often during the grazing period. Although theoretical considerations developed in this study should be validated in the field, this research showed the potential use of a test-day model for analyzing functional traits to advise dairy farmers. [less ▲]Detailed reference viewed: 101 (35 ULg)
Ground-Target Tracking in Multiple Cameras Using Collaborative Particle Filters and Principal Axis-Based Integration
Du, Wei ; ; Verly, Jacques et al
in IPSJ Transactions on Computer Vision and Applications (2009), 1Detailed reference viewed: 91 (4 ULg)
Fast Gas Chromatography Characterisation Of Purified Semiochemicals From Essential Oils Of Matricaria Chamomilla L. (Asteraceae) And Nepeta Cataria L. (Lamiaceae)
Heuskin, Stéphanie ; ; Leroy, Pascal et al
in Journal of Chromatography. A (2009), 1216(14), 2768-2775Detailed reference viewed: 257 (89 ULg)
Expression of the "glanded-plant and glandless-seed" trait of Australian diploid cottons in different genetic backgrounds.
; Lognay, Georges ; et al
in Euphytica : International Journal of Plant Breeding (2009), 165(2), 211-221Detailed reference viewed: 260 (24 ULg)
Adsorption of essential oil components of Xylopia aethiopica (Annonaceae) by kaolin from Wak, Adamawa province (Cameroon).
; ; et al
in Applied Clay Science (2009), 44(1-2), 1-6
Essential oils of aromatic plants are nowadays cited as suitable tools for better protection of stored grains from insect pest attacks. These chemical insecticides are less attractive to producers because ... [more ▼]
Essential oils of aromatic plants are nowadays cited as suitable tools for better protection of stored grains from insect pest attacks. These chemical insecticides are less attractive to producers because of their low persistence and difficulty to use as pure product. There is therefore a need to formulate themas an easy handling chemical with better persistence. The present work aimed to study the formulation through adsorption of active components of Xylopia aethiopica Dunal (Annonaceae) essential oil on kaolin. X. aethiopica essential oil was obtained by hydrodistillation in a Clevenger type apparatus unit. Essential oil obtained was analysed by a GC–FID and a GC–MS. The adsorption measurements were performed with collected two kaolin fractions (F1 <100 μm; F2 <50 μm) and two kaolin fractions after H2O2 treatment (F3 <50 μm; F4 <2 μm). The kaolin was characterized by XRD, XRF, FTIR; its specific surface area was determined by the B.E.T method. The amount of essential oil adsorbed was inversely proportional to the particles size. Treatment of kaolin with hydrogen peroxide increased the adsorption capacity of essential oil components. The components adsorbed in highest amounts were sabinene, β-pinene, α-pinene and β-phellandrene. [less ▲]Detailed reference viewed: 170 (9 ULg)
Non, les cabinets d'avocats ne sont pas des banques données!
Jacobs, Ann ;
in Revue de Jurisprudence de Liège, Mons et Bruxelles (2009), 19
Etat de la jurisprudence belge en matière de perquisitions dans les cabinets d'avocats; critique de cette jurisprudence au regard de la jurisprudence de la Cour européenne des droits de l'homme et ... [more ▼]
Etat de la jurisprudence belge en matière de perquisitions dans les cabinets d'avocats; critique de cette jurisprudence au regard de la jurisprudence de la Cour européenne des droits de l'homme et propositions de changements. [less ▲]Detailed reference viewed: 73 (5 ULg)
Transition from turbulent to nearly laminar vortex flow in superconductors with periodic pinning
; Silhanek, Alejandro ; et al
in Physical Review. B : Condensed Matter (2009), 80(14),
We revisit the vortex dynamics in Al thin films containing an artificial periodic array of antidots by means of electrical transport measurements. We clearly identify a turbulent to laminarlike vortex ... [more ▼]
We revisit the vortex dynamics in Al thin films containing an artificial periodic array of antidots by means of electrical transport measurements. We clearly identify a turbulent to laminarlike vortex flow transition which manifests itself as a negative differential resistivity. This transition is accompanied by a strong irreversibility in the voltage-current characteristics. The dynamical phase diagrams obtained as a function of commensurability, temperature, and driving force are in good agreement with the early predictions by Reichhardt et al. [Phys. Rev. Lett. 78, 2648 (1997)] based on molecular dynamic simulations. [less ▲]Detailed reference viewed: 7 (1 ULg)
Développement de l'entre-soi communautaire dans les espaces résidentiels périurbains
in Déviance et Société (2009), 33Detailed reference viewed: 95 (27 ULg)