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See detailPBP5 complementation of a PBP3 deficiency in Enterococcus hirae
Leimanis, S.; Hoyez, N.; Hubert, S et al

in Journal of Bacteriology (2006), 188(17), 6298-6307

The low susceptibility of enterococci to beta-lactams is due to the activity of the low-affinity penicillin-binding protein 5 (PBP5). One important feature of PBP5 is its ability to substitute for most ... [more ▼]

The low susceptibility of enterococci to beta-lactams is due to the activity of the low-affinity penicillin-binding protein 5 (PBP5). One important feature of PBP5 is its ability to substitute for most, if not all, penicillin-binding proteins when they are inhibited. That substitution activity was analyzed in Enterococcus hirae SL2, a mutant whose pbp5 gene was interrupted by the nisRK genes and whose PBP3 synthesis was submitted to nisin induction. Noninduced SL2 cells were unable to divide except when plasmid-borne pbp5 genes were present, provided that the PBP5 active site was functional. Potential protein-protein interaction sites of the PBP5 N-terminal module were mutagenized by site-directed mutagenesis. The T-167-L-184 region (designated site D) appeared to be an essential intramolecular site needed for the stability of the protein. Mutations made in the two globular domains present in the N-terminal module indicated that they were needed for the suppletive activity. The P-197-N-209 segment (site E) in one of these domains seemed to be particularly important, as single and double mutations reduced or almost completely abolished, respectively, the action of PBP5. [less ▲]

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See detailRemodelage chirurgical du ventricule gauche par endoventriculoplastie: observation clinique
Radermecker, M. A.; Canivet, J. L.; Roediger, Laurence ULg et al

in Revue Médicale de Liège (2006), 61(9), 649-52

A case of dilated ischemic cardiomyopathy, with severely impaired left ventricular systolic function, treated by endoventriculoplasty and CABG, is reported. The authors discuss the modern concepts ... [more ▼]

A case of dilated ischemic cardiomyopathy, with severely impaired left ventricular systolic function, treated by endoventriculoplasty and CABG, is reported. The authors discuss the modern concepts regarding the surgical treatment of large anterior asynergic scars following occlusion of the LAD. Since there are more similarities between akinesia and dyskinesia than previously thought, the endoventriculoplasty of DOR may constitute a new way to surgically remodel the left ventricle in dilated cardiomyopathies with large anterior akinesia. This surgery significantly improves the ventricular function and the overall patients' prognosis at short and mid term follow up. [less ▲]

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See detailLE DEVELOPPEMENT TERRITORIAL DURABLE
Boniver, Véronique ULg; Denis, Olivier; Derzelle, Christophe et al

Report (2006)

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See detailLong-term glycaemic effects of pioglitazone in triple oral therapy: Results from PROactive
Charbonnel, B.; Scheen, André ULg

in Diabetologia (2006, September), 49(Suppl. 1), 488-489

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See detailExtension du modèle de Cascade Intranucléaire de Liège aux énergies comprises entre 2 et 10 GeV
Pedoux, Sophie ULg

Master of advanced studies dissertation (2006)

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See detailSteady flows of a laterally heated ferrofluid layer: Influence of inclined strong magnetic field and gravity level
Hennenberg, M.; Weyssow, B.; Slavtchev, S. et al

in Physics of Fluids (2006), 18(9),

A horizontal ferrofluid layer is submitted to a lateral heating and to a strong oblique magnetic field. The problem, combining the momentum and heat balance equations with the Maxwell equations ... [more ▼]

A horizontal ferrofluid layer is submitted to a lateral heating and to a strong oblique magnetic field. The problem, combining the momentum and heat balance equations with the Maxwell equations, introduces two Rayleigh numbers, Ra the gravitational one and Ram the magnetic one, to represent the buoyancy and the Kelvin forces, which induce motion, versus the momentum viscous diffusion and heat diffusion. Whatever the inclination of the magnetic field, the steady solution of the problem is presented as a power series of a small parameter epsilon(H) measuring the ratio of variation of the magnetization across the layer divided by the magnitude of the external imposed field. For cases of physical relevance, comparisons between analytical and numerical studies have lead to a major statement: in the strong field region (epsilon(H)<< 1) the zero order solution is the product of the Birikh solution that corresponds to the usual Newtonian fluid submitted to a lateral gradient, multiplied by a modulating factor accounting for inclination and both Rayleigh numbers. Physically, this simplified solution is valid for microgravity conditions where the magnetic field competes enough with microgravity effects to invert the laminar flow and thus suppress the motion for two specific values of the inclination angle. (c) 2006 American Institute of Physics. [less ▲]

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See detailDetecting Awareness in the Vegetative State
Owen, Adrian; Coleman, Martin; Boly, Mélanie ULg et al

in Science (2006)

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See detailEarly-type stars in the young open cluster IC 1805. II. The probably single stars HD 15570 and HD 15629, and the massive binary/triple system HD 15558
De Becker, Michaël ULg; Rauw, Grégor ULg; Manfroid, Jean ULg et al

in Astronomy and Astrophysics (2006), 456

Aims.We address the issue of the multiplicity of the three brightest early-type stars of the young open cluster IC 1805, namely HD 15570, HD 15629 and HD 15558. Methods: .For the three stars, we measured ... [more ▼]

Aims.We address the issue of the multiplicity of the three brightest early-type stars of the young open cluster IC 1805, namely HD 15570, HD 15629 and HD 15558. Methods: .For the three stars, we measured the radial velocity by fitting Gaussian curves to line profiles in the optical domain. In the case of the massive binary HD 15558, we also used a spectral disentangling method to separate the spectra of the primary and of the secondary in order to derive the radial velocities of the two components. These measurements were used to compute orbital solutions for HD 15558. Results: .For HD 15570 and HD 15629, the radial velocities do not present any significant trend attributable to a binary motion on time scales of a few days, nor from one year to the next. In the case of HD 15558 we obtained an improved SB1 orbital solution with a period of about 442 days, and we report for the first time on the detection of the spectral signature of its secondary star. We derive spectral types O5.5III(f) and O7V for the primary and the secondary of HD 15558. We tentatively compute a first SB2 orbital solution although the radial velocities from the secondary star should be considered with caution. The mass ratio is rather high, i.e. about 3, and leads to very extreme minimum masses, in particular for the primary object. Minimum masses of the order of 150 ± 50 and 50 ± 15 M_ȯ are found respectively for the primary and the secondary. Conclusions: .We propose that HD 15558 could be a triple system. This scenario could help to reconcile the very large minimum mass derived for the primary object with its spectral type. In addition, considering new and previously published results, we find that the binary frequency among O-stars in IC 1805 has a lower limit of 20%, and that previously published values (80%) are probably overestimated. [less ▲]

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See detailA preliminary study of development of the buccal apparatus in Pomacentridae (Teleostei, Perciformes)
Frederich, Bruno ULg; Parmentier, Eric ULg; Vandewalle, Pierre ULg

in Animal Biology (2006), 56(3), 351-372

This study compares, in five species of Pomacentridae (Chromis viridis, Dascyllus flavicaudus, Chrysiptera glauca, Pomacentrus pavo, Plectroglyphidodon lacrymatus), the changes in cephalic skeletal ... [more ▼]

This study compares, in five species of Pomacentridae (Chromis viridis, Dascyllus flavicaudus, Chrysiptera glauca, Pomacentrus pavo, Plectroglyphidodon lacrymatus), the changes in cephalic skeletal structures between settling larvae and adults living on the reef. Variations were studied in the suspensorium, the opercle, the mandible, the premaxillary bone and the teeth. Comparisons of the feeding apparatus enabled two different types of feeding to be distinguished in adults: suction feeding and grazer sucking. Adult C. viridis and D. flavicaudus have a high suspensorium, a well-rounded mouth during jaw opening and caniniform teeth. These two species can be considered to be suction feeders. On the other hand, adult C glauca, P pavo and P lacrymatus have less developed suspensoria, opercles and ascending process of the premaxillar, and present incisiform teeth. These three species appear to be grazer suckers. However, the larvae of all five species present an unimodal way of feeding, defined as ram-suction. Differences between larvae and adults appear clearly to be related to their ecology. The increasing variation presented in feeding apparatus during the settlement phase could be linked to diversification leading to occupancy of different ecological niches. [less ▲]

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See detailEtude de la pathogénicité de différents variants du viroïde de la mosaïque latente du pêcher (PLMVd) et mise au point d'une technique de détection du phénomène d'extinction post-transcriptionnelle de gènes (Posttranscriptional gene silencing)
Parisi, Olivier ULg

Master's dissertation (2006)

Le Peach latent mosaïc viroïd (PLMVd) est étudié depuis plusieurs années à l’Unité de Phytopathologie. Ces recherches ont permis, entre autres, le développement de techniques moléculaires de détection (RT ... [more ▼]

Le Peach latent mosaïc viroïd (PLMVd) est étudié depuis plusieurs années à l’Unité de Phytopathologie. Ces recherches ont permis, entre autres, le développement de techniques moléculaires de détection (RT-PCR) et de quantification (rétro-transcription suivie de PCR en temps réel) de ce viroïde. Elles ont été utilisées ici pour caractériser la réplication de variants mutés par rapport à un variant de référence non muté. Les mutations réalisées concernent le pseudo-noeud P8 (variant P8) et le domaine ribozyme du viroïde (variant 6A). Ces variants mutés et non mutés ont été inoculés sur des plants de pêcher de la variété GF-305. Les résultats obtenus par quantification absolue du PLMVd au cours des neuf derniers mois ont montré que ces mutations ont eu un impact négatif sur la réplication du viroïde. En moyenne, les titres en PLMVd mesurés ont été respectivement 80 fois inférieurs et 320 fois inférieurs après inoculation par les variants 6A et P8 par rapport aux titres rencontrés dans les plants inoculés par le variant de référence. Ces deux variants mutés ont induit des symptômes de mosaïque sévère dans un premier temps et de mosaïque légère par la suite. Les mutations introduites ont pu amener à la réduction du taux de réplication ou d’auto-clivage de ces variants, favoriser leur dégradation via la RNA silencing et/ou affecter la relation hôte-pathogène. Il est également probable que les variants mutés aient été incapables de se maintenir ou de se répliquer dans les plants mais que des mutations compensatoires soient apparues in vivo et aient restauré leur activité. [less ▲]

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See detailLivre Blanc Céréales - Gembloux - Informations avant les semis
De Proft, Michel; Bodson, Bernard ULg

Book published by Gembloux Agro-Bio Tech - Edition Septembre 2006 (2006)

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See detailCharacterisation of stability behaviour of ultra fine alumina powder in view its colloidal processing by gel-casting
Gaydardzhiev, Stoyan ULg; Ay, P.

in Onal G. et al. (Ed.) Proceedings of XXIII International Mineral Processing Congress, 3-8 September 2006, Istanbul (2006, September)

The paper reports on results study aimed at assessing of the influence of dispersant type on the electrochemical properties of highly concentrated alumina suspension. The relationship between the type of ... [more ▼]

The paper reports on results study aimed at assessing of the influence of dispersant type on the electrochemical properties of highly concentrated alumina suspension. The relationship between the type of dispersant and the stabilization ability towards ultrafine alumina powder CT3000 has been studied. The effectiveness was determined mainly by the zeta potential value, specific surface charge, the shift in the pH(iep) and by visual controlling the fluidity of the slurry at constant solid loading. A good agreement between the zeta potential and the specific surface charge as a way to determine the optimal dose of dispersant has been documented. Gel-casting experiments showed that the Dolapix CE64, an ammonium polyacrylate dispersant was the best dispersant for the studied powder. [less ▲]

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See detailImprovement of the Theta(+) width estimation method on the light cone
Lorce, Cédric ULg

in Physical Review D (2006), 74(5),

Recently, Diakonov and Petrov have suggested a formalism in the chiral quark soliton model allowing one to derive the 3-, 5-, 7-, ...quark wave functions for the octet, decuplet, and antidecuplet. They ... [more ▼]

Recently, Diakonov and Petrov have suggested a formalism in the chiral quark soliton model allowing one to derive the 3-, 5-, 7-, ...quark wave functions for the octet, decuplet, and antidecuplet. They have used this formalism and many strong approximations in order to estimate the exotic Theta(+) width. The latter has been estimated to similar to 4 MeV. Besides they obtained that the 5-quark component of the nucleon is about 50% of its 3-quark component meaning that relativistic effects are not small. We have improved the technique by taking into account some relativistic corrections and considering the previously neglected 5-quark exchange diagrams. We also have computed all nucleon axial charges. It turns out that exchange diagrams affect very little Diakonov's and Petrov's results while relativistic corrections reduce the Theta(+) width to similar to 2 MeV and the 5- to 3-quark component of the nucleon ratio to 30%. [less ▲]

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See detailPrecursors to avalanches in a granular monolayer
Scheller, T.; Huss, C.; Lumay, Geoffroy ULg et al

in Physical Review E (2006), 74(3, Pt 1), 31311

We investigate the stability of a granular monolayer composed of spherical grains on an inclined plate. When the tilt angle alpha increases, some reorganizations are observed throughout the pile. The ... [more ▼]

We investigate the stability of a granular monolayer composed of spherical grains on an inclined plate. When the tilt angle alpha increases, some reorganizations are observed throughout the pile. The packing fraction rho of the packing evolves by successive jumps. Those discontinuous events precede the collapse of the pile at a critical angle alphac. The occurrence of precursors before avalanches is modeled by stop-and-go motions of blocks due to the competition between sliding friction and the Janssen effect [J. Durand, (Springer-Verlag, New York, 2000)]. [less ▲]

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See detailActivités économiques et intérêt local : mesure des flux et identification des localisations optimales
Bastin, A.; Fontaine, P.; Istaz, D. et al

Report (2006)

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See detailA cross-sectional study of adiponectin in patients with schizophrenia
Hanssens, L.; De Hert, M.; Van Eyck, D. et al

in European Neuropsychopharmacology (2006, September), 16(Suppl. 4), 430-431

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See detailEvaluation of different bone markers in hemodialyzed patients
Cavalier, Etienne ULg; Delanaye, Pierre ULg; Collette, Julien ULg et al

in Clinica Chimica Acta (2006), 371(1-2), 107-111

Background: Routinely, nephrologists rely on different biochemical markers like intact PTH (iPTH), bone-specific alkaline phosphatase (BALP), plasmatic calcium and phosphate. The aim of the present study ... [more ▼]

Background: Routinely, nephrologists rely on different biochemical markers like intact PTH (iPTH), bone-specific alkaline phosphatase (BALP), plasmatic calcium and phosphate. The aim of the present study was to evaluate different other bone markers like N-terminal propeptide of type I procollagen (PINP), active isoform 5b of the tartrate-resistant acid phosphatase (TRAP 5b) and beta-crossLaps (R) (CTXS) as well as full-length PTH (wPTH), presumed non-(1-84) PTH, and their ratio in the diagnosis of renal osteodystrophy with high and low turnover. We also determined 25 hydroxyvitamin D (25VTD), 1-25 dihydroxyvitamin D and homocystein (HCY). Methods: We performed those parameters on 73 patients with end-stage renal disease according to the manufacturers' instructions. Results: There were very strong correlations between the bone markers concentrations, particularly between BALP and PINP (r=0.953). We did not observe any correlation between the ratio whole PTH/non-(1-84) PTH and any of the usual bone markers. This ratio was significantly (p < 0.05) higher in low and high bone turnover patients than in normal patients according to the K/DOQI. We found a correlation between low levels of 25VTD and high levels of HCY Conclusions: BALP offers the best clinical and analytical profile as the easier marker of choice in hemodiallyzed patients for the diagnosis of bone disease. (c) 2006 Elsevier B.V All rights reserved. [less ▲]

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