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See detailThe Tolerability of Risk
Delvenne, Pierre ULg; Brunet, Sébastien ULg

in Journal of Risk Research (2008)

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See detailADAMTS-1 metalloproteinase induces a stromal reaction and propotes tumor development in mice.
Rocks, Natacha ULg; Paulissen, Geneviève ULg; Quesada Calvo, Florence ULg et al

in Cancer Research (2008), 68(22), 9541-50

ADAMTS-1 (a disintegrin and metalloproteinase with thrombospondin motifs), the first described member of the ADAMTS family, is differentially expressed in various tumors. However, its exact role in tumor ... [more ▼]

ADAMTS-1 (a disintegrin and metalloproteinase with thrombospondin motifs), the first described member of the ADAMTS family, is differentially expressed in various tumors. However, its exact role in tumor development and progression is still unclear. The aim of this study was to investigate the effects of ADAMTS-1 transfection in a bronchial epithelial tumor cell line (BZR) and its potential to modulate tumor development. ADAMTS-1 overexpression did not affect in vitro cell properties such as (a) proliferation in two-dimensional culture, (b) proliferation in three-dimensional culture, (c) anchorageindependent growth in soft agar, (d) cell migration and invasion in modified Boyden chamber assay, (e) angiogenesis in the aortic ring assay, and (f) cell apoptosis. In contrast, ADAMTS-1 stable transfection in BZR cells accelerated the in vivo tumor growth after s.c. injection into severe combined immunodeficient mice. It also promoted a stromal reaction characterized by myofibroblast infiltration and excessive matrix deposition. These features are, however, not observed in tumors derived from cells overexpressing a catalytically inactive mutant of ADAMTS-1. Conditioned media from ADAMTS-1–overexpressing cells display a potent chemotactic activity toward fibroblasts. ADAMTS-1 overexpression in tumors was associated with increased production of matrix metalloproteinase-13, fibronectin, transforming growth factor B (TGF-B), and interleukin-1B (IL-1B). Neutralizing antibodies against TGF-B and IL-1B blocked the chemotactic effect of medium conditioned by ADAMTS-1–expressing cells on fibroblasts, showing the contribution of these factors in ADAMTS-1–induced stromal reaction. In conclusion, we propose a new paradigm for catalytically active ADAMTS-1 contribution to tumor development, which consists of the recruitment of fibroblasts involved in tumor growth and tumor-associated stroma remodeling. [less ▲]

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See detailthe metalloproteinase ADAM-12 regulates bronchial epithelial cell proliferation and apoptosis.
Rocks, Natacha ULg; Estrella, C.; Paulissen, Geneviève ULg et al

in Cell Proliferation (2008), 41(6), 988-1001

Objectives: The ADAMs (a disintegrin and metalloproteinase) enzymes compose a family of membrane-bound proteins characterized by their multi-domain structure and ADAM-12 expression is elevated in human ... [more ▼]

Objectives: The ADAMs (a disintegrin and metalloproteinase) enzymes compose a family of membrane-bound proteins characterized by their multi-domain structure and ADAM-12 expression is elevated in human non-small cell lung cancers. The aim of this study was to investigate the roles played by ADAM-12 in critical steps of bronchial cell transformation during carcinogenesis. Materials and methods: To assess the role of ADAM-12 in tumorigenicity, BEAS-2B cells were transfected with a plasmid encoding human full-length ADAM-12 cDNA, and then the effects of ADAM-12 overexpression on cell behaviour were explored. Treatment of clones with heparin-binding epidermal growth factor (EGF)-like growth factor (HB-EGF) neutralizing antibodies as well as an EGFR inhibitor allowed the dissection of mechanisms regulating cell proliferation and apoptosis. Results: Overexpression of ADAM-12 in BEAS-2B cells promoted cell proliferation. ADAM-12 overexpressing clones produced higher quantities of HB-EGF in their culture medium which may rely on membrane-bound HB-EGF shedding by ADAM-12. Targeting HB-EGF activity with a neutralizing antibody abrogated enhanced cell proliferation in the ADAM-12 overexpressing clones. In sharp contrast, targeting of amphiregulin, EGF or transforming growth factor-α failed to influence cell proliferation; moreover, ADAM-12 transfectants were resistant to etoposide-induced apoptosis and the use of a neutralizing antibody against HB-EGF activity restored rates of apoptosis to be similar to controls. Conclusions: ADAM-12 contributes to enhancing HB-EGF shedding from plasma membranes leading to increased cell proliferation and reduced apoptosis in this bronchial epithelial cell line. [less ▲]

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See detailLe Conseil de la concurrence accepte les engagements proposés par les NMPP pour permettre l’accès des MLP au tronc commun du logiciel Presse 2000 moyennant quelques modifications et précisions
Sibony, Anne-Lise ULg

in Concurrences : Revue des Droits de la Concurrence (2008), 2

Cons. conc, déc. n° 08-D-04, 25 février 2008, relative à des pratiques mises en œuvre par les Nouvelles Messageries de la presse parisienne)

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See detailExpression of zebrafish pax6b in pancreas is regulated by two enhancers containing highly conserved cis-elements bound by PDX1, PBX and PREP factors
Delporte, F. M.; Pasque, Vincent; Devos, Nathalie et al

in BMC Developmental Biology (2008), 8

BACKGROUND: PAX6 is a transcription factor playing a crucial role in the development of the eye and in the differentiation of the pancreatic endocrine cells as well as of enteroendocrine cells. Studies on ... [more ▼]

BACKGROUND: PAX6 is a transcription factor playing a crucial role in the development of the eye and in the differentiation of the pancreatic endocrine cells as well as of enteroendocrine cells. Studies on the mouse Pax6 gene have shown that sequences upstream from the P0 promoter are required for expression in the lens and the pancreas; but there remain discrepancies regarding the precise location of the pancreatic regulatory elements. RESULTS: Due to genome duplication in the evolution of ray-finned fishes, zebrafish has two pax6 genes, pax6a and pax6b. While both zebrafish pax6 genes are expressed in the developing eye and nervous system, only pax6b is expressed in the endocrine cells of the pancreas. To investigate the cause of this differential expression, we used a combination of in silico, in vivo and in vitro approaches. We show that the pax6b P0 promoter targets expression to endocrine pancreatic cells and also to enteroendocrine cells, retinal neurons and the telencephalon of transgenic zebrafish. Deletion analyses indicate that strong pancreatic expression of the pax6b gene relies on the combined action of two conserved regulatory enhancers, called regions A and C. By means of gel shift assays, we detected binding of the homeoproteins PDX1, PBX and PREP to several cis-elements of these regions. In constrast, regions A and C of the zebrafish pax6a gene are not active in the pancreas, this difference being attributable to sequence divergences within two cis-elements binding the pancreatic homeoprotein PDX1. CONCLUSION: Our data indicate a conserved role of enhancers A and C in the pancreatic expression of pax6b and emphasize the importance of the homeoproteins PBX and PREP cooperating with PDX1, in activating pax6b expression in endocrine pancreatic cells. This study also provides a striking example of how adaptative evolution of gene regulatory sequences upon gene duplication progressively leads to subfunctionalization of the paralogous gene pair. [less ▲]

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See detailA novel formulation of inhaled doxycycline reduces allergen-induced inflammation, hyperresponsiveness and remodeling by matrix metalloproteinases and cytokines modulation in a mouse model of asthma
Guéders, Maud ULg; Bertholet, P.; Perin, Fabienne ULg et al

in Biochemical Pharmacology (2008), 75(2), 514-26

Background In this study, we assess the effectiveness of inhaled doxycycline, a tetracycline antibiotic displaying matrix metalloproteinases (MMP) inhibitory effects to prevent allergen-induced ... [more ▼]

Background In this study, we assess the effectiveness of inhaled doxycycline, a tetracycline antibiotic displaying matrix metalloproteinases (MMP) inhibitory effects to prevent allergen-induced inflammation, hyperresponsiveness and remodeling. MMPs play key roles in the complex cascade of events leading to asthmatic phenotype. Methods Doxycycline was administered by aerosols by the mean of a novel formulation as a complex with hydroxypropyl-gamma-cyclodextrin (HP-gamma-CD) used as an excipient. BALB/c mice (n = 16–24 in each group) were sensitized and exposed to aerosolized ovalbumin (OVA) from day 21 to 27 (short-term exposure protocol) or 5 days/odd weeks from day 22 to 96 (long-term exposure protocol). Results In the short-term exposure model, inhaled doxycycline decreased allergen-induced eosinophilic inflammation in bronchoalveolar lavage (BAL) and in peribronchial areas, as well as airway hyperresponsiveness. In lung tissue, exposure to doxycycline via inhaled route induced a fourfold increase in IL-10 levels, a twofold decrease in IL-5, IL-13 levels and diminished MMP-related proteolysis and the proportion of activated MMP-9 as compared to placebo. In the long-term exposure model, inhaled doxycycline significantly decreased the extent of glandular hyperplasia, airway wall thickening, smooth muscle hyperplasia and subepithelial collagen deposition which are well recognized features of airway remodeling. Conclusion Doxycycline administered by aerosols decreases the allergen-induced airway inflammation and hyperresponsiveness and inhibits the development of bronchial remodeling in a mouse model of asthma by modulation of cytokines production and MMP activity. [less ▲]

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See detailL’Avocat général Colomer estime que le refus partiel de livrer opposé par un laboratoire à des grossistes en vue d’assécher le commerce parallèle n’est pas un abus per se, mais l’intention manifestement anticoncurrentielle crée une forte présomption d’abus
Sibony, Anne-Lise ULg

in Concurrences : Revue des Droits de la Concurrence (2008), 2

Conclusions de l’avocat général D. Ruiz-Jarabo Colomer, 1er avril 2008, Lélos e.a./Glaxosmithkline, C468/06 à C478/06 L’Avocat général Colomer estime que le refus partiel de livrer opposé par un ... [more ▼]

Conclusions de l’avocat général D. Ruiz-Jarabo Colomer, 1er avril 2008, Lélos e.a./Glaxosmithkline, C468/06 à C478/06 L’Avocat général Colomer estime que le refus partiel de livrer opposé par un laboratoire à des grossistes en vue d’assécher le commerce parallèle n’est pas un abus per se, mais l’intention manifestement anticoncurrentielle crée une forte présomption d’abus [less ▲]

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See detailSox10 is not necessary for auditory neurons survival
Breuskin, I; Bodson, M; Thelen, Nicolas ULg et al

Poster (2008)

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See detail« Maar dus ik denk dat … » Het gebruik van dus en maar door Franstalige leerders van het Nederlands: een kwalitatieve analyse
Perrez, Julien ULg

in Verslagen en Mededelingen van de Koninklijke Vlaamse Academie voor Taal en Letterkunde (2008)

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See detailDesertification process in the sahelian belt of West Africa: What's the state of play?
Hountondji, Yvon ULg; Sokpon, Nestor; Nicolas, Jacques ULg et al

in Ozer, André; Swinne, Danielle (Eds.) Desertification: Migration, Health, Remediation and Local Governance (2008)

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See detailAngles corniculaires et nombres superréels
Bair, Jacques ULg; Henry, Valérie ULg

in Bulletin of the Belgian Mathematical Society Simon Stevin (2008), 15(1), 77-86

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See detailL'assurance obligatoire de la responsabilité des architectes et le principe d'égalité
Kohl, Benoît ULg

in Revue Générale de Droit Civil Belge = Tijdschrift voor Belgisch Burgerlijk (2008)

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See detailL'actualité du droit des ententes en 2007
Petit, Nicolas ULg

in Les Petites Affiches (2008)

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See detailUn projet ambitieux : l'informatisation du Französisches Etymologisches Wörterbuch
Renders, Pascale ULg

in Wallonnes : Chronique de la Société de Langue et de Littérature Wallonnes (2008), (3e trimestre), 3-5

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See detailSelective growth of CdTe on patterned CdTe/Si(211)
Seldrum, T.; Bommena, R.; Samain, Louise ULg et al

in Journal of Vacuum Science & Technology : Part B (2008), 26(3), 1105-1109

The authors have studied selective growth of cadmium telluride on Si(211) by molecular beam epitaxy (MBE). Patterned substrates were produced by optical lithography of MBE-grown CdTe/As/Si(211 ... [more ▼]

The authors have studied selective growth of cadmium telluride on Si(211) by molecular beam epitaxy (MBE). Patterned substrates were produced by optical lithography of MBE-grown CdTe/As/Si(211). Photoemission microscopy was used as the main tool to study selective growth. This is very powerful because Si or SiO2 can be very easily distinguished from areas covered with even small amounts of CdTe due to contrast from work function differences. It was found that CdTe grows on CdTe without sticking on bare Si areas if the temperature is sufficiently high. Based on the analysis of the temperature dependence of the growth rate of CdTe, we suggest that different physisorption energies on Si and CdTe are the main cause of this selective growth. (C) 2008 American Vacuum Society. [less ▲]

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See detailStress echocardiography expert consensus statement: European Association of Echocardiography (EAE) (a registered branch of the ESC).
Sicari, Rosa; Nihoyannopoulos, Petros; Evangelista, Arturo et al

in European journal of echocardiography : the journal of the Working Group on Echocardiography of the European Society of Cardiology (2008), 9(4), 415-37

Stress echocardiography is the combination of 2D echocardiography with a physical, pharmacological or electrical stress. The diagnostic end point for the detection of myocardial ischemia is the induction ... [more ▼]

Stress echocardiography is the combination of 2D echocardiography with a physical, pharmacological or electrical stress. The diagnostic end point for the detection of myocardial ischemia is the induction of a transient worsening in regional function during stress. Stress echocardiography provides similar diagnostic and prognostic accuracy as radionuclide stress perfusion imaging, but at a substantially lower cost, without environmental impact, and with no biohazards for the patient and the physician. Among different stresses of comparable diagnostic and prognostic accuracy, semisupine exercise is the most used, dobutamine the best test for viability, and dipyridamole the safest and simplest pharmacological stress and the most suitable for combined wall motion coronary flow reserve assessment. The additional clinical benefit of myocardial perfusion contrast echocardiography and myocardial velocity imaging has been inconsistent to date, whereas the potential of adding - coronary flow reserve evaluation of left anterior descending coronary artery by transthoracic Doppler echocardiography adds another potentially important dimension to stress echocardiography. New emerging fields of application taking advantage from the versatility of the technique are Doppler stress echo in valvular heart disease and in dilated cardiomyopathy. In spite of its dependence upon operator's training, stress echocardiography is today the best (most cost-effective and risk-effective) possible imaging choice to achieve the still elusive target of sustainable cardiac imaging in the field of noninvasive diagnosis of coronary artery disease. [less ▲]

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See detailBiodiversité linguistique
Durand, Pascal ULg

E-print/Working paper (2008)

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See detailStrain accommodation by slow slip and dyking in a youthful continental rift, East Africa
Calais, E.; d'Oreye, N.; Albaric, J. et al

in Nature (2008), 456

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