Publications ORBi OA
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See detailEstimation of surface soil organic matter by means of hyperspectral data analysis.
Touré, Souleymane; Tychon, Bernard ULg

Conference (2003, September 04)

This presentation shows results from two different hyperspectral instruments for the assessment of soil organic content of agricultural soils in southern Belgium.

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See detailPortable Holographic Marking Unit
Habraken, Serge ULg; vandormael, Denis; Holman, John, C.

Patent (2003)

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See detailDifferent mechanisms are implicated in ERBB2 gene overexpression in breast and in other cancers
Vernimmen, D.; Guéders, Maud ULg; Pisvin, Sonia ULg et al

in British Journal of Cancer (2003), 89(5), 899-906

The ERBB2 gene is overexpressed in 30% of breast cancers and this has been correlated with poor prognosis. ERBB2 is upregulated in other cancers such as prostate, pancreas, colon and ovary. In breast ... [more ▼]

The ERBB2 gene is overexpressed in 30% of breast cancers and this has been correlated with poor prognosis. ERBB2 is upregulated in other cancers such as prostate, pancreas, colon and ovary. In breast cancer cells, the mechanisms leading to ERBB2 gene overexpression are increased transcription and gene amplification. In these cancers, AP-2 transcription factors are involved in ERBB2 overexpression, and AP-2 levels are correlated with p185(c-erbB-2) levels. In this work, we wanted to know if the same molecular mechanisms are responsible for the ERBB2 upregulation in non-breast cancers. We compared ERBB2 gene copy number, p185(c-erbB-2) and mRNA levels with AP-2 levels in several ovary, prostate, colon and pancreas cancer cells. A moderate expression of erbB-2 mRNA and protein were observed in some cells without gene amplification. In contrast to breast cancer cells, AP-2 factors were absent or low in some non-breast cells which did express ERBB2. It is thus likely that AP-2 is not a major player in the increased levels of erbB-2 transcripts in non-breast cancer cells. The transcriptional activity of the ERBB2 promoter in colon and ovary cancer cells was estimated using reporter vectors. The results showed that the promoter regions involved in ERBB2 gene overexpression in breast cancer cells are different from those that lead to the gene upregulation in colon and ovary cancers. In conclusion, our results indicate that different transcriptional and post-transcriptional mechanisms are responsible for the increased levels of erbB-2 transcript and protein in breast and non-breast cancer cells. [less ▲]

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See detailSeparation of the retinal and central contribution to changes in visual performance after light exposure
Vandewalle, Gilles ULg

Master of advanced studies dissertation (2003)

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See detailElaboration d'outils intégrés de gestion des données topographiques et morphologiques pour la modélisation des crues et inondations
Detrembleur, Sylvain ULg

Master of advanced studies dissertation (2003)

L’activité de recherche dont bénéficie ce travail a consisté à poursuivre le développement du logiciel quasi-tridimensionnel de modélisation des écoulements de surface de la suite WOLF. Plus ... [more ▼]

L’activité de recherche dont bénéficie ce travail a consisté à poursuivre le développement du logiciel quasi-tridimensionnel de modélisation des écoulements de surface de la suite WOLF. Plus particulièrement, ce travail vise à développer et à rendre opérationnelle une filière automatique de traitement de données topographiques et morphologiques directement exploitables par WOLF2D. Ces algorithmes sont basés sur des approches hydrauliques ou sédimentaires, afin de générer une information complète sur grids réguliers organisés en blocs. Il s’agit d’exploiter des informations parcellaires, soit acquises par laser ou sonar multi-faisceaux, soit issues de campagnes plus classiques d’acquisition de profils en travers par méthodes topographiques. Une adaptation des lois classiques de frottement est également suggérée et traitée numériquement afin de tenir compte de ce nouveau type d’information très dense ainsi que des obstacles locaux constitués par les ouvrages d’art imposant des mises en charge et des pertes de charge locales. Le bien-fondé de ces développements est mis en lumière par le routage de grosses applications réelles pour lesquelles des campagnes complémentaires sur sites, l’exploitation de laisses de crue locales et des enquêtes de terrain permettent des comparaisons significatives. [less ▲]

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See detailExpression pattern of metalloproteinases and tissue inhibitors of matrix-metalloproteinases in cycling human endometrium
Goffin, Frédéric ULg; Munaut, Carine ULg; Frankenne, Francis et al

in Biology of Reproduction (2003), 69(3), 976-984

The cyclic growth, differentiation, and cell death of endometrium represents the most dynamic example of steroid-driven tissue turnover in human adults. Key effectors in these processes-matrix ... [more ▼]

The cyclic growth, differentiation, and cell death of endometrium represents the most dynamic example of steroid-driven tissue turnover in human adults. Key effectors in these processes-matrix metalloproteinases (MMPs) and their specific inhibitors (TIMPs)-are regulated by ovarian steroids and, locally, by cytokines. We used reverse transcription-polymerase chain reaction to evaluate the expression of both transcriptionally regulated molecules such as estrogen receptor-alpha, progesterone receptor, and prolactin and a large array of MMPs and TIMPs (MMP-1, -2, -3, -7, -8, -9, -11, -12, -19, -26, MT1-MMP, MT2-MMP, MT3-MMP, TIMP-1, -2, -3). Altogether, three distinct patterns of MMP and two patterns of TIMP expression were detected in cycling endometrium: 1). MMPs restricted to the menstrual period (MMPs-1, -3, -8, -9, -12); 2). MMPs and TIMPs expressed throughout the cycle (MMP-2, MT1-MMP, MT2-MMP, MMP-19, TIMP-1, and TIMP-2); 3). MMPs predominantly expressed during the proliferative phase (MMP-7, MMP-11, MMP-26, and MT3-MMP); and 4). TIMP-3, which, contrary to the other TIMPs, shows significant modulations, with maximum expression during the late secretory and menstrual phases. These specific patterns of MMP expression associated with each phase of the cycle may point to specific roles in the processes of menstruation, housekeeping activities, angiogenesis, tissue growth, and extracellular matrix remodeling. [less ▲]

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See detailStress echocardiography in horses: comparison of cardiac output during incremental dobutamine infusion in atropinised and non-atropinised ponies
Sandersen, Charlotte ULg; Detilleux, Johann ULg; Delguste, Catherine ULg et al

in Proceedings of the 42d Annual Congress of the British Equine Veterinairy Association (2003, September)

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See detailMoritella cold-active dihydrofolate reductase: Are there natural limits to optimization of catalytic efficiency at low temperature?
Xu, Y.; Feller, Georges ULg; Gerday, Charles ULg et al

in Journal of Bacteriology (2003), 185(18), 5519-5526

Adapting metabolic enzymes of microorganisms to low temperature environments may require a difficult compromise between velocity and affinity. We have investigated catalytic efficiency in a key metabolic ... [more ▼]

Adapting metabolic enzymes of microorganisms to low temperature environments may require a difficult compromise between velocity and affinity. We have investigated catalytic efficiency in a key metabolic enzyme (dihydrofolate reductase) of Moritella profunda sp. nov., a strictly psychrophilic bacterium with a maximal growth rate at 2degreesC or less. The enzyme is monomeric (M-r = 18,291), 55% identical to its Escherichia coli counterpart, and displays T-m and denaturation enthalpy changes much lower than E. coli and Thermotoga maritima homologues. Its stability curve indicates a maximum stability above the temperature range of the organism, and predicts cold denaturation below 0degreesC. At mesophilic temperatures the apparent K-m value for dihydrofolate is 50- to 80-fold higher than for E. coli, Lactobacillus casei, and T. maritima dihydrofolate reductases, whereas the apparent K-m value for NADPH, though higher, remains in the same order of magnitude. At 5degreesC these values are not significantly modified. The enzyme is also much less sensitive than its E. coli counterpart to the inhibitors methotrexate and trimethoprim. The catalytic efficiency (k(cat)/K-m) with respect to dihydrofolate is thus much lower than in the other three bacteria. The higher affinity for NADPH could have been maintained by selection since NADPH assists the release of the product tetrahydrofolate. Dihydrofolate reductase adaptation to low temperature thus appears to have entailed a pronounced trade-off between affinity and catalytic velocity. The kinetic features of this psychrophilic protein suggest that enzyme adaptation to low temperature may be constrained by natural limits to optimization of catalytic efficiency. [less ▲]

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See detailDe LAT-relatie tussen strip en literatuur. Over de 'verstripping' van literaire werken
Meesters, Gert ULg

in Ons Erfdeel (2003), 46(4), 523-530

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See detailQualitative and quantitative criteria for comfortable urban public spaces
Reiter, Sigrid ULg; De Herde, André

in Carmeliet, Jan; Hens, Hugo; Vermeir, Gerrit (Eds.) Research in building physics. Proceedings of the 2nd International Conference on Building Physics (2003, September)

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See detailInvestigation of singlet oxygen reactivity towards propofol
Heyne, Belinda; Kohnen, Stephan ULg; Brault, Daniel et al

in Photochemical & Photobiological Sciences (2003), 2(9), 939-945

The reaction between the anaesthetic agent 2,6-diisopropylphenol (propofol, PPF) and singlet oxygen (1O2) has been investigated in aqueous solution by means of HPLC, GC, absorption spectroscopy and laser ... [more ▼]

The reaction between the anaesthetic agent 2,6-diisopropylphenol (propofol, PPF) and singlet oxygen (1O2) has been investigated in aqueous solution by means of HPLC, GC, absorption spectroscopy and laser flash photolysis with infrared luminescence detection. The rate constants for the physical and chemical quenching of 1O2 by PPF (kPPF) are found to be 2.66 x 10(5) M(-1) s(-1) and approximately 3.2 x 10(6) M(-1) s(-1) in CD3OD and D2O-CD3OD (75:25 v/v), respectively. The reaction of propofol with singlet oxygen produced by light irradiation of Rose Bengal leads essentially to two reaction products, 2,6-diisopropyl-p-benzoquinone and 3,5,3',5'-tetraisopropyl-(4,4')-diphenoquinone that are unambiguously identified from comparison with authentic samples. [less ▲]

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See detailStructure of liquid semiconductors
Gaspard, Jean-Pierre ULg; Raty, Jean-Yves ULg

in Journal de Physique IV (2003), 111

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See detailThe in situ Glyptostroboxylon forest of Hoegaarden (Belgium) at the Initial Eocene Thermal Maximum (55 Ma)
Fairon-Demaret, Muriel ULg; Steurbaut, Etienne; Damblon, Freddy ULg et al

in Review of Palaeobotany and Palynology (2003), 126(1-2), 103-129

Hundreds of silicified standing stumps have been discovered within a lignitic horizon in the middle of the Tienen Formation near Hoegaarden in northeast Belgium. The anatomical features of the fossil ... [more ▼]

Hundreds of silicified standing stumps have been discovered within a lignitic horizon in the middle of the Tienen Formation near Hoegaarden in northeast Belgium. The anatomical features of the fossil stumps, as those of the numerous silicified secondary xylem remains collected since the last century from this area, demonstrate that they all belong to a single taxodiaceous taxon. The stumps bear characteristics shared by Taxodioxylon gypsaceum and Glyptostroboxylon tenerum, but affinities with the latter appear closer. They are attributed to Glyptostroboxylon sp. Calibration of the sedimentological, stratigraphical and organic carbon isotope data reveals that these taxodiaceous fossil trees developed in a swampy lowland environment most probably during the Initial Eocene Thermal Maximum at ca. 55 Ma. (C) 2003 Elsevier B.V. All rights reserved. [less ▲]

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See detailLearned material content and acquisition level modulate cerebral reactivation during posttraining rapid-eye-movements sleep
Peigneux, Philippe ULg; Laureys, Steven ULg; Fuchs, Sonia et al

in NeuroImage (2003), 20(1), 125-134

We have previously shown that several brain areas are activated both during sequence learning at wake and during subsequent rapid-eye-movements (REM) sleep (Nat. Neurosci. 3 (2000) 831-836), suggesting ... [more ▼]

We have previously shown that several brain areas are activated both during sequence learning at wake and during subsequent rapid-eye-movements (REM) sleep (Nat. Neurosci. 3 (2000) 831-836), suggesting that REM sleep participates in the reprocessing of recent memory traces in humans. However, the nature of the reprocessed information remains open. Here, we show that regional cerebral reactivation during posttraining REM sleep is not merely related to the acquisition of basic visuomotor skills during prior practice of the serial reaction time task, but rather to the implicit acquisition of the probabilistic rules that defined stimulus sequences. Moreover, functional connections between the reactivated cuneus and the striatum-the latter being critical for implicit sequence learning-are reinforced during REM sleep after practice on a probabilistic rather than on a random sequence of stimuli. Our results therefore support the hypothesis that REM sleep is deeply involved in the reprocessing and optimization of the high-order information contained in the material to be learned. In addition, we show that the level of acquisition of probabilistic rules attained prior to sleep is correlated to the increase in regional cerebral blood flow during subsequent REM sleep. This suggests that posttraining cerebral reactivation is modulated by the strength of the memory traces developed during the learning episode. Our data provide the first experimental evidence for a link between behavioral performance and cerebral reactivation during REM sleep. (C) 2003 Elsevier Inc. All rights reserved. [less ▲]

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See detailConférence citoyenne sur les tests génétiques "Lire dans mes gènes" - Rapport d'évaluation
Mormont, Marc ULg; Melard, François ULg

Report (2003)

Rapport d'évaluation sur la première conférence citoyenne nationale en Belgique (FRB). Le rapport présente les implications méthodologiques liées au fonctionnement de la conférence citoyenne.

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See detailDurability of mortars modified with metakaolin
Courard, Luc ULg; Darimont, Anne ULg; Schouterden, M. et al

in Cement & Concrete Research (2003), 33(9), 1473-1479

Results of an investigation to determine the effects of metakaolin additions on transport properties of mortars are reported. Comparisons are made to ordinary Portland cement (OPC) to determine the ... [more ▼]

Results of an investigation to determine the effects of metakaolin additions on transport properties of mortars are reported. Comparisons are made to ordinary Portland cement (OPC) to determine the influence of addition and replacement percentage. Cement is replaced on a mass basis of 5-20% for metakaolin. A mixture with natural kaolin is also studied. The transport properties and chemical behaviors are analyzed by means of chloride diffusion tests and sulfate immersion. Obserdvations after more than 100 days are used to prescribe mixtures that reduce the rate of chloride diffusion and sulfate degradation. For metakaolin, the optimum seems to be between 10% and 15% with regard to inhibition effect on chloride diffusion and sulfate attack. (C) 2003 Elsevier Ltd. All rights reserved. [less ▲]

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See detailA tool to design steel elements submitted to compartment fires - OZone V2. Part 2: Methodology and application
Cadorin, Jean-François; Pintea, Dan; Dotreppe, Jean-Claude ULg et al

in Fire Safety Journal (2003), 38(5), 429-451

The methodology implemented in the tool OZone V2 to design steel elements submitted to compartment fires is presented. Input needed to define a fire compartment are first quoted. The procedure to define ... [more ▼]

The methodology implemented in the tool OZone V2 to design steel elements submitted to compartment fires is presented. Input needed to define a fire compartment are first quoted. The procedure to define the design fire is then explained. This procedure enables to take into account the benefits of active measures on the fire safety. The combined use of a two- and a one-zone model is then presented. A particular attention is given to the criteria of choice of the model and to the different scenarios that can occur. The calculation of steel element temperature is then explained. The proposed procedure takes into account the localised effect of a fire with the help of Hasemi's model. The fire resistance is then calculated with the EUROCODE 3 methods. An application is finally presented. (C) 2003 Elsevier Science Ltd. All rights reserved. [less ▲]

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