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See detailBacillus amyloliquefaciens GA1 as a source of potent antibiotics and other secondary metabolites for biocontrol of plant pathogens.
Arguelles Arias, Anthony ULg; Ongena, Marc ULg; Halimi, Badre et al

in Microbial Cell Factories (2009), 8(1), 63

Phytopathogenic fungi affecting crop and post-harvested vegetables are a amajor threat to food production and food storage. To face these drawbacks, producers have become increasingly dependent on ... [more ▼]

Phytopathogenic fungi affecting crop and post-harvested vegetables are a amajor threat to food production and food storage. To face these drawbacks, producers have become increasingly dependent on agrochemicals. However, intensive use of these compounds has led to the emergence of pathogen resistance and severe negative environmental impacts. There are also a number of plant diseases for which chemical solutions are ineffective or non-existent as well as an increasing demand by consumers for pesticide-free food. Thus, biological control through the use of natural antagonistic microorganisms has emerged as a promising alternative to chemical pesticides for more rational and safe crop management. RESULTS: The genome of the plant-associated B. amyloliquefaciens GA1 was sample sequenced. Several gene clusters involved in the synthesis of biocontrol agents were detected. Four gene clusters were shown to direct the synthesis of the cyclic lipopeptides surfactin, iturin A and fengycin as well as the iron-siderophore bacillibactin. Beside these non-ribosomaly synthetised peptides, three additional gene clusters directing the synthesis of the antibacterial polyketides macrolactin, bacillaene and difficidin were identified. Mass spectrometry analysis of culture supernatants led to the identification of these secondary metabolites, hence demonstrating that the corresponding biosynthetic gene clusters are functional in strain GA1. In addition, genes encoding enzymes involved in synthesis and export of the dipeptide antibiotic bacilysin were highlighted. However, only its chlorinated derivative, chlorotetaine, could be detected in culture supernatants. On the contrary, genes involved in ribosome-dependent synthesis of bacteriocin and other antibiotic peptides were not detected as compared to the reference strain B. amyloliquefaciens FZB42. CONCLUSION: The production of all of these antibiotic compounds highlights B. amyloliquefaciens GA1 as a good candidate for the development of biocontrol agents. [less ▲]

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See detailNon-Standard economics and competition law
Sibony, Anne-Lise ULg

Conference (2009, November 26)

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See detailLa copropriété d'immeubles ou de groupe d'immeubles bâtis : un tour d'horizon de la réforme
Salvé, Arianne ULg

in Revue de la Faculté de Droit de l'Université de Liège (2009), 2009/4

La Chambre des représentants a donné son feu vert à la modification des dispositions légales relatives à la copropriété (d’immeubles ou de groupes d’immeubles bâtis). La Commission Justice de la Chambre a ... [more ▼]

La Chambre des représentants a donné son feu vert à la modification des dispositions légales relatives à la copropriété (d’immeubles ou de groupes d’immeubles bâtis). La Commission Justice de la Chambre a eu à cœur de simplifier et d’accroître la transparence dans la gestion des copropriétés par rapport au prescrit légal de la loi du 30 juin 1994. Entre-temps, le Sénat a utilisé son droit d’évocation du projet de loi voté à la Chambre. Un sommaire du projet de réforme. (Lge 25/11/2009) [less ▲]

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See detailProperty Tax and Land Use Management in Vietnam
Trinh, Hong Loan ULg

Scientific conference (2009, November 24)

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See detailPenser double. Foucault, Lévy-Bruhl et l'ethnologie
Bolmain, Thomas ULg

Scientific conference (2009, November 24)

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See detailHomme, environnement, climat: chronique d'une catastrophe annoncée?
Ozer, Pierre ULg

Conference given outside the academic context (2009)

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See detailThe mayonnaise droplet
Terwagne, Denis ULg; Gilet, Tristan ULg; Vandewalle, Nicolas ULg et al

Conference (2009, November 23)

A compound drop is made of a millimetric water drop encapsulated by an oil shell. They are obtained by merging one drop of each component (water and oil). Afterwards, they are laid on a high viscosity oil ... [more ▼]

A compound drop is made of a millimetric water drop encapsulated by an oil shell. They are obtained by merging one drop of each component (water and oil). Afterwards, they are laid on a high viscosity oil bath which is vertically vibrated. When the forcing acceleration is higher than a given threshold, compound drops can bounce on the surface. We show that above a second threshold some oil contained in the shell enters in the inner water droplet. In a second experiment, we drop the compound droplet on the oil bath at rest. We can determine the range of impact speed in which capillary waves developed on the surface are able to generate an oil drop (coming from the shell) in the water drop. When the bouncing trajectories of the droplets are analyzed a correlation between the emulsion threshold and the static analysis can be made. [less ▲]

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See detailInter- and intraday variability of Flemish travel behavior
Cools, Mario ULg

Doctoral thesis (2009)

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See detailReconstruire la ville sur la ville
Ozer, Pierre ULg

Article for general public (2009)

Nous vivons une époque formidable ! Notre planète, cernée par des centaines de satellites d’observation de la Terre, est sous monitoring constant. Le développement extraordinaire des technologies de ... [more ▼]

Nous vivons une époque formidable ! Notre planète, cernée par des centaines de satellites d’observation de la Terre, est sous monitoring constant. Le développement extraordinaire des technologies de traitement de l’information nous permet de suivre, en direct, l’évolution de l’état de santé de l’environnement de notre village planétaire. Et, dans le domaine des constats, il n’y a plus de place au doute. Ainsi, nous savons que la biodiversité disparaît ici et ailleurs. Nous savons que les forêts, essentiellement dans les pays en développement, se contractent inexorablement. Nous savons que le réchauffement du système climatique est sans équivoque et que son origine anthropique est établie. Nous savons aussi qu’il va fragiliser nos économies en impactant les ressources en eau, les écosystèmes, les zones littorales, la souveraineté alimentaire de larges régions, la santé publique, et les plus vulnérables d’entre nous en accroissant les inégalités sociales ici et ailleurs (réfugiés climatiques) en fonction du degré d’adaptation à ces nouvelles contraintes inéluctables. [...] [less ▲]

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See detailIMMUNOCAP© ISAC: INTEREST IN ALLERGY DIAGNOSIS
Gadisseur, Romy ULg; Chapelle, Jean-Paul ULg; Cavalier, Etienne ULg

Poster (2009, November 19)

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See detailCRD and technical validation of ImmunoCAPISAC
Gadisseur, Romy ULg; Chapelle, Jean-Paul ULg; Cavalier, Etienne ULg

Conference (2009, November 19)

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See detailFunctionlization and grafting of polylactide by click chemistry
Riva, Raphaël ULg; Croisier, Florence ULg; Jérôme, Christine ULg et al

Conference (2009, November 19)

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See detailDéveloppements alimentaires: la demande guide l'innovation
Blecker, Christophe ULg

Scientific conference (2009, November 18)

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See detailVaricella-Zoster Virus IE4 Protein Interacts with SR Proteins and Exports mRNAs through the TAP/NXF1 Pathway.
Ote, Isabelle ULg; Vandevenne, Patricia ULg; Bontems, Sébastien ULg et al

in PLoS ONE (2009), 4(11), 7882

Available data suggest that the Varicella-Zoster virus (VZV) IE4 protein acts as an important regulator on VZV and cellular genes expression and could exert its functions at post-transcriptional level ... [more ▼]

Available data suggest that the Varicella-Zoster virus (VZV) IE4 protein acts as an important regulator on VZV and cellular genes expression and could exert its functions at post-transcriptional level. However, the molecular mechanisms supported by this protein are not yet fully characterized. In the present study, we have attempted to clarify this IE4-mediated gene regulation and identify some cellular partners of IE4. By yeast two-hybrid and immunoprecipitation analysis, we showed that IE4 interacts with three shuttling SR proteins, namely ASF/SF2, 9G8 and SRp20. We positioned the binding domain in the IE4 RbRc region and we showed that these interactions are not bridged by RNA. We demonstrated also that IE4 strongly interacts with the main SR protein kinase, SRPK1, and is phosphorylated in in vitro kinase assay on residue Ser-136 contained in the Rb domain. By Northwestern analysis, we showed that IE4 is able to bind RNA through its arginine-rich region and in immunoprecipitation experiments the presence of RNA stabilizes complexes containing IE4 and the cellular export factors TAP/NXF1 and Aly/REF since the interactions are RNase-sensitive. Finally, we determined that IE4 influences the export of reporter mRNAs and clearly showed, by TAP/NXF1 knockdown, that VZV infection requires the TAP/NXF1 export pathway to express some viral transcripts. We thus highlighted a new example of viral mRNA export factor and proposed a model of IE4-mediated viral mRNAs export. [less ▲]

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