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See detailComparative ontogeny, processing, and segmental distribution of the renal chloride channel, ClC-5.
JOURET, François ULg; Igarashi, Takashi; Gofflot, Francoise et al

in Kidney International (2004), 65(1), 198-208

BACKGROUND: The renal chloride channel ClC-5, which is responsible for Dent's disease, is coexpressed with the vacuolar H+-ATPase in proximal tubules (PT) and alpha-type intercalated cells (IC) of the ... [more ▼]

BACKGROUND: The renal chloride channel ClC-5, which is responsible for Dent's disease, is coexpressed with the vacuolar H+-ATPase in proximal tubules (PT) and alpha-type intercalated cells (IC) of the mature kidney. Neonatal cases of Dent's disease suggest that ClC-5 distribution must be acquired before birth. However, the ontogeny of ClC-5, and its processing and segmental distribution with respect to related proteins during nephrogenesis remain unknown. METHODS: Immunoblotting, real-time polymerase chain reaction (RT-PCR), immunostaining, and deglycosylation studies were used to investigate the expression, distribution, and maturation of ClC-5 during mouse and human nephrogenesis, in comparison with H+-ATPase, type II carbonic anhydrase (CAII), and aquaporin-1 (AQP1). RESULTS: An early induction (E13.5-E14.5) of ClC-5 was observed in mouse kidney, with persistence at high levels through late nephrogenesis. This pattern contrasted with the progressive expression of H+-ATPase and AQP1, and the postnatal upregulation of CAII. Immunostaining showed expression of ClC-5 in ureteric buds and, from E14.5, its location in developing PT. From E15.5, ClC-5 codistributed with H+-ATPase in PT cells and alpha-type IC. In the human kidney, ClC-5 was detected from 12 gestation weeks; its distribution was similar to that observed in mouse, except for a later detection in IC. Although mouse and human ClC-5 proteins are glycosylated, biochemical differences between fetal and adult proteins were observed in both species. CONCLUSION: The segmental expression of ClC-5 and H+-ATPase is essentially achieved during early nephrogenesis, in parallel with the onset of glomerular filtration. These data give insight into PT and IC maturation, and explain early phenotypic variants of Dent's disease. [less ▲]

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See detailRapport final de synthèse du projet 4M-µPompe (2001-2003). Annexes Partie 1/2 (sections 1-2-3/1,2,3,4,5)
Raucent, Benoit; Dereine, E.; Smal, Olivier et al

Report (2004)

Micropump : Modelling, Design, Drawings, Tests, Publications, Manufacturing, Medical aspects, Miscellaneous aspects.

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See detailLaboratory investigation of inlays and surface treatments for the decoration of copper based alloy objects from the imperial roman period
Mathis, François ULg; Descamps, Sophie; Robcis, Dominique et al

in 34th INTERNATIONAL SYMPOSIUM ON ARCHAEOMETRY (2004)

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See detailEvaluation of population density of Pichia anomala strain K and Candida oleophila strain O and their protection against Penicillium expansum Link on apples.
Lahlali, Rachid; Jijakli, Haissam ULg

in Bulletin OILB/SROP = IOBC/WPRS Bulletin (2004), 27(8), 341-346

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See detailEfficacy assessment of Candida oleophila (strain O) and Pichia anomala (strain K) against major postharvest diseases of citrus fruits in Morocco.
Lahlali, R.; Serrhini, M. N.; Jijakli, Haissam ULg

in comm. appl. biol. sci. Ghent University (2004), 69(4), 601-609

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See detailParalysie non traumatique du nerf interosseux antébrachial postérieur liée à la pratique du VTT
Goffinet, Estelle; Zeevaert, Bernard; Claes, Frédérique et al

in Journal de Traumatologie du Sport (2004)

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See detailLe Codex miscellaneus chez les Frères Croisiers. Technique de copie et vie spirituelle (XVe-XVIe siècles)
Opsomer, Carmélia ULg

in Segno e Testo. International Journal of Manuscripts and Text Transmission (2004), 2

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See detailFemmes qui rêvent avec les doigts, les voies de la peinture aborigènes d'Australie
Strivay, Lucienne ULg

in Cahiers Internationaux de Symbolisme (2004), 107-108-109

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See detailDes socles de compétences à la mise en place d'une démarche expérimentale à l'école primaire : intervention en formation initiale des enseignants
Hindryckx, Marie-Noëlle ULg; Graftiau, Marie-Christine; Stegen, Pierre

in Actes du Troisième Congrès des chercheurs e, Education (Re)trouver le plaisir d'enseigner et d'apprendre ; construire savoirs et compétences (2004)

recherche action menée en collaboration entre deux services universitaires et la Haute Ecole Charlemagne de Liège autour de la formation initiale des enseignants du primaire en sciences. Interventions ... [more ▼]

recherche action menée en collaboration entre deux services universitaires et la Haute Ecole Charlemagne de Liège autour de la formation initiale des enseignants du primaire en sciences. Interventions dans le cadre des ateliers de formation professionnelle [less ▲]

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See detailDinámicas locales de la conservación in situ de la diversidad de tubérculos andinos en el microcentro Candelaria
Terrazas, A. F.; Baudoin, Jean-Pierre ULg; Duran, G.

Conference given outside the academic context (2004)

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See detailEcogeography, demography, diversity and conservation of Phaseolus lunatus L. in the Central Valley of Costa Rica.
Baudoin, Jean-Pierre ULg; Rocha, O.; Degreef, J. et al

Book published by International Plant Genetic Resources Institute (IPGRI) (2004)

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See detailEtude histologique du développement d'embryons issus du croisement Phaseolus vulgaris L. x P. coccineus L.
NGuema Ndoutoumou, P.; Toussaint, André ULg; Baudoin, Jean-Pierre ULg

Conference given outside the academic context (2004)

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See detailDynamiques locales de la conservation in situ à la ferme de la diversité de tubercules andins dans le micro-réservoir de Candelaria
Toussaint, André ULg; Terrazas, A.; Malice, Marie ULg et al

Conference given outside the academic context (2004)

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See detailConservation ex situ de tubérculos andinos y analisis de datos de la diversidad genética de la oca (Oxalis tuberosa Mol.)
Ugarte, M.; Baudoin, Jean-Pierre ULg

Conference given outside the academic context (2004)

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See detailEffect Of Imbalance Between Energy And Nitrogen Supplies On Microbial Protein Synthesis And Nitrogen Metabolism In Growing Double-Muscled Belgian Blue Bulls
Valkeners, Damien; Thewis, André ULg; Piron, Fabien ULg et al

in Journal of Animal Science (2004), 82(6), 1818-1825

Six double-muscled Belgian Blue bulls (initial weight: 345 ± 16 kg) with cannulas in the rumen and proximal duodenum were used in two juxtaposed 3 × 3 Latin squares to study the effect of a lack of ... [more ▼]

Six double-muscled Belgian Blue bulls (initial weight: 345 ± 16 kg) with cannulas in the rumen and proximal duodenum were used in two juxtaposed 3 × 3 Latin squares to study the effect of a lack of synchronization between energy and N in the rumen on microbial protein synthesis and N metabolism by giving the same diet according to three different feeding patterns. The feed ingredients of the diet were separated into two groups supplying the same amount of fermentable OM (FOM), but characterized by different levels of ruminally degradableN (RDN). The first group primarily provided energy for the ruminal microbes (14.6 g of RDN/kg of FOM), and the second provided N (33.3 g of RDN/kg of FOM). These two groups were fed to the bulls simultaneously or alternately with the aim of creating three different time periods of imbalance (0, 12, or 24 h) between energy and N supplies in the rumen. The introduction of imbalance affected neither microbial-N flow at the duodenum (P = 0.65) nor efficiency of growth (P = 0.69), but decreased (P = 0.016) the NDF degradation in the rumen 12.2% for a 12-h period of imbalance. N retention was not affected by imbalance (P = 0.53) and reached 57.8, 58.5, and 54.7 g/d, respectively, for 0-, 12- and 24-h imbalance. It seems that the introduction of an imbalance of 12 or 24 h between energy and N supplies for the ruminal microbes by altering the feeding pattern of the same diet does not negatively influence microbial protein synthesis or N retention by the animal. Nitrogen recycling in the rumen plays a major role in regulating the amount of ruminally availableNand allows for continuous synchronization of N- and energy-yielding substrates for the microorganisms in the rumen. Therefore, a lack of synchronization in the diet between the energy and N supplies for the ruminal microbes is not detrimental to their growth or for the animal as long as the nutrient supply is balanced on a 48-h basis. Thus, these dietary feeding patterns may be used under practical feeding conditions with minimal effect on the performance of ruminant animals. [less ▲]

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See detailEarly abortion in reciprocal crosses between phaseolus vulgaris and phaseolus polyanthus, and in vitro culture of immature embryos from these species
Toussaint, André ULg; Geerts, P.; Clement, F. et al

in Belgian Journal of Botany (2004), 137(1), 47-54

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See detailDistribución geográfica y relaciones genéticas del género Arracacia Bancroft en el Perú
Blas Sevillano, R.; Arbizu, C.; Baudoin, Jean-Pierre ULg

Conference given outside the academic context (2004)

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