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See detailComparison of the Sensititre YeastOne and Fungitest methods with the NCCLS M27-A2 reference method for antifungal susceptibility testing of yeasts
Hayette, Marie-Pierre ULg; Kondarowski, E.; Melin, Pierrette ULg et al

Poster (2005, December)

Background: The recent introduction of Sensititre YeastOne, a colorimetric microdilution method that includes new antifungal agents has opened the field to MIC’s determination by an easy-to-perform method ... [more ▼]

Background: The recent introduction of Sensititre YeastOne, a colorimetric microdilution method that includes new antifungal agents has opened the field to MIC’s determination by an easy-to-perform method. The aim of this study was to compare this test with the NCCLS M27-A protocol and with Fungitest, a current routine method for yeasts susceptibility testing. Methods: Sensititre YeastOne method (Trek diagnostic), and the NCCLS M27-A2 were performed on 300 yeasts clinical isolates distributed as follow: 125 C. albicans, 273 non-albicans species. Four antifungal agents were tested by the reference method: amphotericine B (AmB), fluconazole (FZ), itraconazole (ITZ), and voriconazole (VOR). The reading of the Sensititre and NCCLS results was visually performed after 24 and 48 h respectively. The Fungitest (Biorad) method was applied on 121 among the 300 isolates and the reading was made after 24 to 48 h incubation time according to the positive control growth. Results: By the NCCLS method, the MICs50/MICs90 (µg/ml) were as follows: 1/2 (AmB); 16/64 (FZ); 0.25/4 (ITZ) and 0.125/2 (VOR). Sensititre vs. NCCLS: The overall agreement within 2 dilutions for AmB, FZ, ITZ and VOR was respectively 54, 82, 80 and 78%. The MICs50/MICs90 were in absolute concordance for VOR by both techniques. Very major errors (%) were recorded as follows: 0.01/0 (AmB with a MIC ≥ 4/8µg/ml for resistant strains respectively), 1.6 (FZ), 3.6 (ITZ) and 2.3 (VOR with a MIC ≥ 8µg/ml for resistant strains). Fungitest vs. NCCLS: The agreement between both methods including minor discrepancies was 98% (AmB), 88% (FZ) and 98% (ITZ). Following the breakpoints given by the manufacturer, very major errors were 6.3% for FZ, 0.03% for ITZ and none for AmB. Conclusions: Sensititre is a convenient alternative to the NCCLS method for yeast susceptibility testing. Fungitest in spite of good correlations must change the breakpoints and include new antifungal agents to be competitive. [less ▲]

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See detailEULAR report on the use of ultrasonography in painful knee osteoarthritis. Part 1: Prevalence of inflammation in osteoarthritis
D'Agostino, M. A.; Conaghan, P.; Le Bars, M. et al

in Annals of the Rheumatic Diseases (2005), 64(12), 1703-1709

Objectives: To assess the prevalence of inflammation in subjects with chronic painful knee osteoarthritis (OA), as determined by the presence of synovitis or joint effusion at ultrasonography (US); and to ... [more ▼]

Objectives: To assess the prevalence of inflammation in subjects with chronic painful knee osteoarthritis (OA), as determined by the presence of synovitis or joint effusion at ultrasonography (US); and to evaluate the correlation between synovitis, effusion, and clinical parameters. Methods: A cross sectional, multicentre, European study was conducted under the umbrella of EULAR-ESCISIT. Subjects had primary chronic knee OA (ACR criteria) with pain during physical activity >= 30 mm for at least 48 hours. Clinical parameters were collected by a rheumatologist and an US examination of the painful knee was performed by a radiologist or rheumatologist within 72 hours of the clinical examination. Ultrasonographic synovitis was defined as synovial thickness >= 4 mm and diffuse or nodular appearance, and a joint effusion was defined as effusion depth >= 4 mm. Results: 600 patients with painful knee OA were analysed. At US 16 (2.7%) had synovitis alone, 85 (14.2%) had both synovitis and effusion, 177 (29.5%) had joint effusion alone, and 322 (53.7%) had no inflammation according to the definitions employed. Multivariate analysis showed that inflammation seen by US correlated statistically with advanced radiographic disease (Kellgren-Lawrence grade >= 3; odds ratio (OR) = 2.20 and 1.91 for synovitis and joint effusion, respectively), and with clinical signs and symptoms suggestive of an inflammatory "flare'', such as joint effusion on clinical examination (OR = 1.97 and 2.70 for synovitis and joint effusion, respectively) or sudden aggravation of knee pain (OR = 1.77 for joint effusion). Conclusion: US can detect synovial inflammation and effusion in painful knee OA, which correlate significantly with knee synovitis, effusion, and clinical parameters suggestive of an inflammatory "flare''. [less ▲]

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See detailControl of puberty by excitatory amino acid neurotransmitters and its clinical implications
Parent, Anne-Simone ULg; Matagne, V.; Bourguignon, Jean-Pierre ULg

in Endocrine (2005), 28(3), 281-285

Excitatory amino acids, glutamate in particular, have a marked stimulatory effect on the reproductive axis, particulary at puberty. Glutamate, N-methyl-D-aspartate (NMDA), and kainate stimulate ... [more ▼]

Excitatory amino acids, glutamate in particular, have a marked stimulatory effect on the reproductive axis, particulary at puberty. Glutamate, N-methyl-D-aspartate (NMDA), and kainate stimulate gonadotropin-releasing hormone (GnRH) secretion in immature mammals and NMDA receptor stimulation results in precocious puberty in rats and monkeys. Puberty is characterized by an increased sensitivity of GnRH to glutamate as well as an increase in glutaminase activity in the hypothalamus. Glutamatergic and GABAergic regulation of GnRH secretion seem strongly interdependent around puberty. in addition to the transsynaptic glutamatergic regulation of GnRH secretion, a coordinated activity of glutamatergic neurons and astroglial cells has been shown to play an active role in puberty. The participation of kainate receptors in the estradiol-induced advancement of puberty suggest that these receptors may be involved in the estradiol-mediated activation of GnRH secretion at puberty. A case of precocious puberty associated with hyperglycinemia illustrates the NMDA involvement in puberty in humans. in this patient, the occurrence of precocious puberty was thought to result from excessive stimulation by glycine of the NMDA receptors linked to the GnRH neurons. Glutamate plays several roles in the hypothalamic mechanism of puberty as it has been shown in animal models, but there are still few clinical data supporting the role of glutamate in human puberty. [less ▲]

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See detailIntegration de la pharmacogenetique dans la pratique medicale
Dideberg, Vinciane ULg; Bours, Vincent ULg

in Revue Médicale de Liège (2005), 60(12), 918-22

Today, the initiation of any medical treatment still raises questions about its efficacy and safety. Indeed, therapeutic responses vary over time and between individuals and are influenced by age, sex ... [more ▼]

Today, the initiation of any medical treatment still raises questions about its efficacy and safety. Indeed, therapeutic responses vary over time and between individuals and are influenced by age, sex, other treatments and the pathology itself. Genetic factors are thought to be responsible for 20 to 95% of these individual variations. Recent advances in biotechnology, molecular genetics and genomics allow a better understanding of drug metabolism and action. Pharmacogenetics, compiling phenotypic and genotypic data, may lead to a more personalized treatment. To allow a better approach of pharmacogenetics and pharmacogenomics, we will define these two terms and describe their actual and future clinical application. [less ▲]

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See detailPotential benefits of laparoscopic aorto-bifemoral bypass surgery
Rouers, Anthony; MEURISSE, Nicolas; LAVIGNE, Jean-Paul ULg et al

in Acta Chirurgica Belgica (2005), 105(6), 610-615

Background: This series aims to prove the positive impact of laparoscopic approach in aortofemoral bypass grafting. Methods : It concerns a retrospective non randomized study comparing 58 consecutive ... [more ▼]

Background: This series aims to prove the positive impact of laparoscopic approach in aortofemoral bypass grafting. Methods : It concerns a retrospective non randomized study comparing 58 consecutive patients treated with laparoscopic procedure (n = 30) and with a standard open procedure (n = 28) in a single center. The different operating times, the complications and the follow-up of these two groups are compared Results : The demographics and angiographic data of the two groups were comparable. Operating time was longer in the laparoscopic group. However, we noticed a significant shorter hospitalisation stay (p < 0.0001) after the laparoscopic procedure with a mean 5.1 days. There was no significant difference of morbidity. Conclusion : We suggest that the trans-peritoneal approach is the best way in laparoscopic procedure in term of exposure and ergonomics. Laparoscopic aortofemoral bypass grafting is feasible, safe and effective. Shortening of operating time is observed as surgeon's experience grows. [less ▲]

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See detailPatterns of physical activity determined by heart rate monitoring among diabetic children
Massin, M. M.; Lebrethon, M. C.; Brumioul, Danielle ULg et al

in Archives of Disease in Childhood (2005), 90(12), 1223-1226

Background: Children with type 1 diabetes should be encouraged to participate in physical activity because exercise can benefit insulin sensitivity and improve known risk factors for atherosclerosis ... [more ▼]

Background: Children with type 1 diabetes should be encouraged to participate in physical activity because exercise can benefit insulin sensitivity and improve known risk factors for atherosclerosis. Methods: Physical activity patterns of 127 children and adolescents with stable type 1 diabetes were investigated by 24 hour continuous heart rate monitoring. The percentage of heart rate reserve was used to measure the amounts of physical activity at different intensities. The results were compared with normative data. Results: Diabetic preschoolchildren accumulated 192.7 (78.1), 39.1 (24.3), and 21.3 (9.4) minutes/day (mean (SD)) of light, moderate, and vigorous physical activity, respectively. At the same activity levels, diabetic schoolchildren accumulated 168.9 (76.7), 37.9 (15.9), and 19.0 (14.8) minutes/day, and diabetic teenagers accumulated 166.3 (67.5), 45.6 (26.9), and 25.2 (15.3) minutes/day. Diabetic schoolchildren were significantly more active than healthy peers when considering moderate activity; diabetic teenagers were significantly more active when considering moderate and vigorous activity. There was a negative correlation between the most recent glycated haemoglobin and the time spent in light activities in schoolchildren, and a negative correlation between mean glycated haemoglobin for one year and time spent in light and moderate activities in schoolchildren. Conclusion: The majority of our diabetic patients meet the classical paediatric guidelines for physical activity and compare favourably with their healthy peers. [less ▲]

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See detailAspects zootechniques et économiques de l’utilisation des feuilles de Vitellaria paradoxa et de tourteau de coton en supplémentation sur la production laitière de la vache Borgou en saison hivernale
Chabi Toko, Roukayath ULg

Master's dissertation (2005)

Experiment was carried out to investigate the effects of cottonseed meal (CSM) and Vitellaria paradoxa leaves in rainy season supplementation on milk yield, composition of the milk, live weight changes ... [more ▼]

Experiment was carried out to investigate the effects of cottonseed meal (CSM) and Vitellaria paradoxa leaves in rainy season supplementation on milk yield, composition of the milk, live weight changes and their economical returns. To examine these effects, fifteen (15) Borgou cattle, in late lactation were offered three (3) dietary treatments: grazing natural pasture (control); the two other treatments received in addition cottonseed meal and Vitellaria paradoxa leaves supplements. The experiment lasted ten (10) weeks, including an adaptation period of two (2) weeks. Average daily milk yield was 946,58 g, 1690,07 g and 1176,89 g for the control, cottonseed meal and Vitellaria paradoxa leaves group respectively. Dietary treatments had a significant (p< 0,05) effect on milk yield during the trial. Only cows supplemented CSM produce significantly more milk than those unsupplemented (increasing of 78,54 %). But there is no differences among supplemented group. Cottonseed meal group produce more than 43,60 % milk yield compared to the second supplemented group. The treatments had a significant effect on total solid and milk ash. But no significant effect was observed on fat and protein content. The over all mean values were 15,12 %, 0,35 %, 5,92 % and 4,13 % respectively for total solid, ash, fat and protein content. There were no significant differences (p<0,05) in live weight changes of supplemented group cows. Further more, calves live weight changes were significantly different. Supplemented groups calves live weight increase was significantly more than those unsupplemented from birth to the end of the trial (212,06 g/d and 219,10 g/d versus 161,1 g/d). From birth to the beginning of the trial, only Vitellaria paradoxa leaves group calves had a significant increase in live weight changes. Only CSM group calves had a significant increase in live weight changes (124,98 g/d versus 53,54 g/d for non supplemented group calves) during the trial. Calves live weight changes from birth to the beginning of the trial were highly significantly correlated with those from birth to the end of the trial for all the experimental herd and for CSM group (r = 0,96 and r = 0,99). In other hand, these two live weight changes were slightly correlated for the control (r = 0,96) and Vitellaria paradoxa group (r = 0,97). A net return analysis shows that cottonseed meal and Vitellaria paradoxa supplementation in rainy season was profitable even if Vitellaria paradoxa was more beneficial. [less ▲]

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See detailDépression et maladies coronariennes: quand les émotions nous brisent le coeur...
Massart, Nicolas ULg; Triffaux, Jean-Marc ULg

in Revue Médicale de Liège (2005), 60(12), 931-8

Depression and cardiovascular diseases represent a major public health problem worldwide. Recent studies have shown that depression is a risk factor for the development of coronary artery disease (CAD) in ... [more ▼]

Depression and cardiovascular diseases represent a major public health problem worldwide. Recent studies have shown that depression is a risk factor for the development of coronary artery disease (CAD) in healthy people and also increases morbidity and mortality in depressed patients with CAD. Others studies have shown that selective serotonin inhibitors (SSRIs) constitute a safe and effective treatment for depressed patients with heart disease. There are also data suggesting that treating depression with SSRIs has a protective role in myocardial infarction and may improve outcomes, including mortality. [less ▲]

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See detailThe neural correlate of (un)awareness: lessons from the vegetative state
Laureys, Steven ULg

in Trends in Cognitive Sciences (2005), 9(12), 556-559

Consciousness has two main components: wakefulness and awareness. The vegetative state is characterized by wakefulness without awareness. Recent functional neuroimaging results have shown that some parts ... [more ▼]

Consciousness has two main components: wakefulness and awareness. The vegetative state is characterized by wakefulness without awareness. Recent functional neuroimaging results have shown that some parts of the cortex are still functioning in 'vegetative' patients. External stimulation, such as a painful stimulus, still activates 'primary' sensory cortices in these patients but these areas are functionally disconnected from 'higher order' associative areas needed for awareness. Such studies are disentangling the neural correlates of the vegetative state from the minimally conscious state, and have major clinical consequences in addition to empirical importance for the understanding of consciousness. [less ▲]

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See detailFeeding ecology of five commercial shark species of the Celtic Sea through stable isotope and trace metal analysis
Domi, Nadège; Bouquegneau, Jean-Marie ULg; Das, Krishna ULg

in Marine Environmental Research (2005), 60(5),

In order to trace their feeding habits, stable carbon and nitrogen isotope ratios (delta(15)N and delta(13)C), as well as trace metal concentrations (Zn, Cd, Fe, Cu, Se and Hg) were analysed in the ... [more ▼]

In order to trace their feeding habits, stable carbon and nitrogen isotope ratios (delta(15)N and delta(13)C), as well as trace metal concentrations (Zn, Cd, Fe, Cu, Se and Hg) were analysed in the tissues of five commercial shark species from the Celtic Sea: the tope shark Galeorhinus galeus, the black-mouthed catshark Galeus melastomus, the starry smooth hound Mustelus asterias, the spiny dogfish Squalus acanthias and the lesser-spotted dogfish Scyliorhinus canicula. Our results were compared to previously described stomach contents and isotopic composition of potential preys. Isotopic ratio delta(15)N suggested that tope sharks fed at a higher trophic level (16.7 parts per thousand in the muscle) than the other species, reflecting its piscivorous diet. The lower values of spiny dogfish (11.6 parts per thousand in the muscle) might be explained, amongst other things, by either its migratory behaviour or its preference for preys from lower trophic levels. Cd and Hg were correlated with isotopic ratios delta(13)C and delta(15)N, and were shown to be diet-related whereas Zn, Fe and Cu seemed much more linked to species-specific metabolism. Although this multidisciplinary approach is revealed as a useful tool for the study of shark ecology, the lack of known trophic fractionation suggests that isotopic data be compared to traditional diet analyses. (c) 2005 Elsevier Ltd. All rights reserved. [less ▲]

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See detailThree year joint space narrowing predicts long term incidence of knee surgery in patients with osteoarthritis: an eight year prospective follow up study
Bruyère, Olivier ULg; Richy, F.; Reginster, Jean-Yves ULg

in Annals of the Rheumatic Diseases (2005), 64(12), 1727-1730

OBJECTIVE: To assess the clinical relevance of mean and minimum femorotibial joint space narrowing (JSN) for predicting future osteoarthritis related surgery in patients with knee osteoarthritis. METHODS ... [more ▼]

OBJECTIVE: To assess the clinical relevance of mean and minimum femorotibial joint space narrowing (JSN) for predicting future osteoarthritis related surgery in patients with knee osteoarthritis. METHODS: 126 subjects with primary knee osteoarthritis were followed prospectively for a mean eight years. Minimum and mean joint space width (JSW) were assessed from standard x rays at baseline and after a follow up of three years. The rate of knee osteoarthritis related surgery was recorded for the following five years. RESULTS: After a mean follow up of eight years, 16 patients (12.7%) had received osteoarthritis related joint surgery. The areas under the curves (AUC) resulting from the receiver operating characteristic curve analyses for predicting osteoarthritis surgery were 0.73 (p=0.006) for minimum JSN and 0.55 (p=0.54) for mean JSN. The cut off for minimum JSN maximising sensitivity and specificity for predicting future surgery was a change of 0.7 mm or more in minimum joint space width over a period of three years. However, no meaningful differences were observed for cut off values between 0.5 and 0.8 mm The relative risk (adjusted for age, body mass index, baseline symptoms, and baseline JSW) of experiencing osteoarthritis related surgery during the eight year of follow up was 5.15 (95% confidence interval, 1.70 to 15.60) (p=0.004) in patients with a minimum joint space narrowing of 0.7 mm or more during the first three years of the study. CONCLUSIONS: A cut off of 0.5 to 0.8 mm in minimum JSN, measured on standard x rays, reflects a clinically relevant progression in patients with knee osteoarthritis. [less ▲]

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See detailHétérogénéité, mon cher souci
Lafontaine, Dominique ULg

in Revue Internationale d'Education (2005), 40

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See detailOverhead power lines real time monitoring : a future trend ?
Lilien, Jean-Louis ULg; Destiné, Jacques ULg

in Revue d'Electricité et d'Electronique Industrielle (2005), 4

Overview of the needs which exist to monitor overhead lines in real time. Application to ampacity is detailed to determine the load available on a given line at a given time. After an overview of the ... [more ▼]

Overview of the needs which exist to monitor overhead lines in real time. Application to ampacity is detailed to determine the load available on a given line at a given time. After an overview of the existing sensors, a new device is proposed (Ampacimon) and first results are detailed on a 70 kV line. [less ▲]

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See detailInfluence of emotion on memory for temporal information
D'Argembeau, Arnaud ULg; Van der Linden, Martial ULg

in Emotion (Washington, D.C.) (2005), 5(4), 503-507

Contextual information. such as color and spatial location, has been found to be better remembered for emotional than for neutral items. The current study examined whether the influence of emotion extends ... [more ▼]

Contextual information. such as color and spatial location, has been found to be better remembered for emotional than for neutral items. The current study examined whether the influence of emotion extends to memory for another fundamental feature of episodic memory: temporal information. Results from a list-discrimination paradigm showed that (a) item memory was enhanced for both negative and positive pictures compared with neutral ones and was better for negative than for positive pictures and (b) temporal information was better remembered for negative than for positive and neutral pictures, whereas positive and neutral pictures did not differ from each other. These findings are discussed in relation to the processes involved in memory for temporal information. [less ▲]

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See detailIsocinétisme et LCA : quelle position du contre-appui résistif ?
Croisier, Jean-Louis ULg; Maquet, Didier ULg; Huskin, Jean-Pierre ULg et al

in Abstract book de la 5ème Journée Belge d’Isocinétisme (2005, December)

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See detailExploration isocinétique et électromyographique des fléchisseurs et extenseurs de genou : application à des populations souffrant de douleurs chroniques
Maquet, Didier ULg; Demoulin, Christophe ULg; Forthomme, Bénédicte ULg et al

in Abstract book de la 5ème Journée Belge d’Isocinétisme, Campus Erasme (2005, December)

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