Publications ORBi OA
Bookmark and Share    
Full Text
Peer Reviewed
See detailLes anticorps monoclonaux en hématologie en 2009
Bonnet, Christophe ULg; Beguin, Yves ULg; De Prijck, Bernard ULg et al

in Revue Médicale de Liège (2009), 64

Directed against the CD20 antigen on B lymphocytes, rituximab (MabThera) is now incorporated in the first line therapy of symptomatic follicular as well as diffuse large B cell non-Hodgkin's lymphoma and ... [more ▼]

Directed against the CD20 antigen on B lymphocytes, rituximab (MabThera) is now incorporated in the first line therapy of symptomatic follicular as well as diffuse large B cell non-Hodgkin's lymphoma and offers superior response and survival rates. 90Y ibritumomab tiuxetan (Zevalin) combines the specificity of rituximab for the CD20 antigen and the therapeutic effect of β irradiation. Given in monotherapy, it constitutes an interesting alternative therapy for follicular lymphomas in second relapse. Alemtuzumab (MabCampath) recognizes the CD52 antigen and offers encouraging results in chronic lymphocytic leukemia resistant to classical chemotherapy. [less ▲]

Detailed reference viewed: 251 (14 ULg)
Full Text
See detailA Treasured Family Album
Ledent, Bénédicte ULg

in Wasafiri (2009), 59

Detailed reference viewed: 49 (6 ULg)
Full Text
Peer Reviewed
See detailCombining microsatellite and pedigree data to estimate relationships among Skyros ponies.
Bömcke, Elisabeth ULg; Gengler, Nicolas ULg

in Journal of Applied Genetics (2009), 50(2), 133-43

Relationship coefficients are particularly useful to improve genetic management of endangered populations. These coefficients are traditionally based on pedigree data, but in case of incomplete or ... [more ▼]

Relationship coefficients are particularly useful to improve genetic management of endangered populations. These coefficients are traditionally based on pedigree data, but in case of incomplete or inexistent pedigrees they are replaced by coefficients calculated from molecular data. The main objective of this study was to develop a new method to estimate relationship coefficients by combining molecular with pedigree data, which is useful for specific situations where neither pedigree nor molecular data are complete. The developed method was applied to contribute to the conservation of the Skyros pony breed, which consists of less than 200 individuals, divided into 3 main herds or subpopulations. In this study, relationships between individuals were estimated using traditional estimators as well as the newly developed method. For this purpose, 99 Skyros ponies were genotyped at 16 microsatellite loci. It appeared that the limitation of the most common molecular-based estimators is the use of weights that assume relationships equal to 0. The results showed that, as a consequence of this limitation, negative relationship values can be obtained in small inbred populations, for example. By contrast, the combined estimator gave no negative values. Using principal component analysis, the combined estimator also enabled a better graphic differentiation between the 3 subpopulations defined previously. In conclusion, this new estimator can be a promising alternative to traditionally used estimators, especially in inbred populations, with both incomplete pedigree and molecular information. [less ▲]

Detailed reference viewed: 52 (9 ULg)
Full Text
See detailLe contrôle des motifs du licenciement et le licenciement abusif des employés
Clesse, Jacques ULg; Mortier, Aurélie ULg

in Le licenciement abusif (2009)

Detailed reference viewed: 235 (39 ULg)
Full Text
Peer Reviewed
See detailInfluence of the rare-earth element on the effects of the structural and magnetic phase transitions in CeFeAsO, PrFeAsO and NdFeAsO
McGuire, Michael A; Hermann, Raphaël ULg; Sefat, Athena S et al

in New Journal of Physics (2009), 11

We present results of transport and magnetic properties and heat capacity measurements on polycrystalline CeFeAsO, PrFeAsO and NdFeAsO. These materials undergo structural phase transitions, spin density ... [more ▼]

We present results of transport and magnetic properties and heat capacity measurements on polycrystalline CeFeAsO, PrFeAsO and NdFeAsO. These materials undergo structural phase transitions, spin density wave-like magnetic ordering of small moments on iron and antiferromagnetic ordering of rare-earth moments. The temperature dependence of the electrical resistivity, Seebeck coefficient, thermal conductivity, Hall coefficient and magnetoresistance are reported. The magnetic behavior of the materials have been investigated using Mossbauer spectroscopy and magnetization measurements. Transport and magnetic properties are affected strongly by the structural and magnetic transitions, suggesting significant changes in the band structure and/or carrier mobilities occur, and phonon-phonon scattering is reduced upon transformation to the low-temperature structure. Results are compared with recent reports for LaFeAsO, and systematic variations in properties as the identity of Ln is changed are observed and discussed. As Ln progresses across the rare-earth series from La to Nd, an increase in the hole contributions to the Seebeck coefficient and increases in magnetoresistance and the Hall coefficient are observed in the low-temperature phase. Analysis of hyperfine fields at the iron nuclei determined from Mossbauer spectra indicates that the moment on Fe in the orthorhombic phase is nearly independent of the identity of Ln, in apparent contrast to reports of powder neutron diffraction refinements. [less ▲]

Detailed reference viewed: 26 (3 ULg)
Full Text
Peer Reviewed
See detailPulmonary veno-occlusive disease in myeloproliferative disorder.
Willems, Evelyne ULg; Canivet, Jean-Luc ULg; Ghaye, Benoît ULg et al

in European Respiratory Journal (2009), 33(1), 213-216

The present study reports a case of biopsy-proven pulmonary veno-occlusive disease as a cause of severe pulmonary hypertension in a patient suffering from a chronic myeloproliferative disorder. The ... [more ▼]

The present study reports a case of biopsy-proven pulmonary veno-occlusive disease as a cause of severe pulmonary hypertension in a patient suffering from a chronic myeloproliferative disorder. The pulmonary disease evolved favourably under treatment with defibrotide, a pro-fibrinolytic medication used in hepatic veno-occlusive disease. [less ▲]

Detailed reference viewed: 84 (27 ULg)
Full Text
Peer Reviewed
See detailToxoplasmore cérébrale compliquant une mini-allogreffe de cellules souches hématopoïétiques du sang périphérique
Dugauquier, C.; Bataille, Y.; Willems, Evelyne ULg et al

in Revue Médicale de Liège (2009), 64(7-8), 366-369

We report the occurrence of a cerebral toxoplasmosis 52 days after a non-myeloablative allogeneic stem cell transplantation as treatment for acute myeloid leukemia.

Detailed reference viewed: 97 (8 ULg)
Full Text
See detailDevelopment of an original approach to evaluate effects of surfactants, biomass and pollutants on the scaling-up of a two-phase partitioning bioreactor
Aldric, Jean-Marc ULg

(2009)

BACKGROUND: Two-phase partitioning bioreactors (TPPBs) are considered as a new technology for xenobiotic degradation in gaseous effluents. However, there is still a need for more knowledge on how to ... [more ▼]

BACKGROUND: Two-phase partitioning bioreactors (TPPBs) are considered as a new technology for xenobiotic degradation in gaseous effluents. However, there is still a need for more knowledge on how to design and scale-up TPPBs. The partitioning of the two phases remains a misunderstood way of research. In particular, the impact of pollutant (isopropylbenzene), biomass and surfactant extract needs to be better evaluated. RESULTS:. An adaptated scale-down apparatus has been developed in order to quantify the speed of phase partitioning (SPP) into a plug flow section. Firstly, it was shown that isopropylbenzene (IPB) doesn’t destabilize more significantly the system. Secondly, respectively 0.5 g.L-1 and 0.05 g.L-1 of biomass and surfactant extract, separately or in mixture, were sufficient to ensure the stability of the two-phase system. Finally, a 100 m3 limit of scaling-up was suggested on the basis of the circulation time comparison. CONCLUSION: The scaling-up of an aqueous/silicone-oil TPPB was found to be definitely conceivable when the presence of biotic compounds were considered. However, further considerations are needed to verify our assumptions, in particular by taking into account the velocity field pattern in full-scale bioreactors and reproduce it in lab-scale apparatus. [less ▲]

Detailed reference viewed: 48 (6 ULg)
Full Text
Peer Reviewed
See detailThe structure of the di-zinc subclass B2 metallo-beta-lactamase CphA reveals that the second inhibitory zinc ion binds in the "histidine" site.
Bebrone, Carine ULg; Delbrück, Heinrich; Kupper, Michaël et al

in Antimicrobial Agents and Chemotherapy (2009)

Bacteria can defend themselves against beta-lactam antibiotics through the expression of class B beta-lactamases, which cleave the beta-lactam amide bond and render the molecule harmless. There are three ... [more ▼]

Bacteria can defend themselves against beta-lactam antibiotics through the expression of class B beta-lactamases, which cleave the beta-lactam amide bond and render the molecule harmless. There are three subclasses of class B beta-lactamases (B1, B2 and B3), all of which require Zn(2+) for activity and can bind either one or two zinc ions. Whereas the B1 and B3 metallo-beta-lactamases are most active as di-zinc enzymes, subclass B2 enzymes such as Aeromonas hydrophila CphA are inhibited by the binding of a second zinc ion. We crystallized A. hydrophila CphA in order to determine the binding site of the inhibitory zinc ion. X-ray data from zinc-saturated crystals allowed us to solve the crystal structures of the di-zinc forms of the wild-type enzyme and N220G mutant. The first zinc ion binds in the "cysteine" site, as previously determined for the mono-zinc form of the enzyme. The second zinc ion occupies a slightly modified "histidine" site, where the conserved His118 and His196 residues act as metal ligands. This atypical coordination sphere probably explains the rather high dissociation constant for the second zinc ion compared to those observed in enzymes of subclasses B1 and B3. Inhibition by the second zinc ion results from immobilization of the catalytically-important His118 and His196 residues, as well as the folding of the Gly232-Asn233 loop into a position that covers the active site. [less ▲]

Detailed reference viewed: 55 (7 ULg)
Full Text
Peer Reviewed
See detailBioreactor mixing efficiency modulates the activity of a prpoS::gfp reporter gene in E. coli
Delvigne, Frank ULg; Boxus, Mathieu ULg; Ingels, Sophie et al

in Microbial Cell Factories (2009), 8(15),

ABSTRACT: BACKGROUND: Extensive studies have shown that up-scaling of bioprocesses has a significant impact on the physiology of the microorganisms. Among the factors associated with the fluid dynamics of ... [more ▼]

ABSTRACT: BACKGROUND: Extensive studies have shown that up-scaling of bioprocesses has a significant impact on the physiology of the microorganisms. Among the factors associated with the fluid dynamics of the bioreactor, concentration gradients induced by loss of the global mixing efficiency associated with the increasing scale is the main phenomena leading to strong physiological modifications at the level of the microbial population. These changes are not fully understood since they involve complex physiological mechanisms. In this work, we intend to investigate, at the single cell level, the expression of the rpoS gene associated with the stress response of E. coli. The cultures of the reporter strain have been performed in a small scale reactor as well as in a series of scaled-down bioreactors able to induce extracellular perturbations with increasing level of magnitude. RESULTS: The rpoS level has been monitored by the aim of a transcriptional reporter gene based on the synthesis of the green fluorescent protein (GFP). It has been observed that the level of GFP increases during the transition from batch to fed-batch phase. After this initial increase, the GFP content of the cell drops, primarily due to the dilution by cell division. However, a significant drop of the GFP content has been observed if using a partitioned bioreactor, for which the mixing conditions are very bad, leading to the exposure of the cells to cyclic and stochastic extracellular fluctuations. If considering the flow cytometric profile of the cell to cell GFP content, this drop has to be attributed to the appearance of segregation at the level of the GFP content among the microbial population. CONCLUSION: The generation of extracellular perturbations (in the present case, at the level of the sugar concentration and the dissolved oxygen level) has led to a drop at the level of the rpoS expression level. This drop has to be attributed to a segregation phenomenon in microbial population, with a major sub-population exhibiting a low expression level and a minor sub-population keeping its initial elevated expression level. The intensity of the segregation, as well as its time of appearance during the culture can be related to the bioreactor mixing efficiency. [less ▲]

Detailed reference viewed: 52 (20 ULg)
Full Text
Peer Reviewed
See detailThymus dysfunction in the development of type 1 diabetes and endocrine autoimmune diseases
Geenen, Vincent ULg; Dardenne Olivier

in European Endocrinology (2009), 5

The discovery that thymic epithelium from many species expresses a large repertoire of genes encoding neuroendocrine and other tissue-restricted antigens has radically changed our knowledge of the ... [more ▼]

The discovery that thymic epithelium from many species expresses a large repertoire of genes encoding neuroendocrine and other tissue-restricted antigens has radically changed our knowledge of the pathogenic mechanisms underlying the development of organ-specific autoimmune diseases such as type 1 diabetes and autoimmune endocrine diseases. Rather than a breakdown of immunological selftolerance in periphery, there is mounting evidence that the diabetogenic autoimmune response may first arise from a thymus dysfunction in the central programming of β-cell self-tolerance. Insulin-like growth factor 2 (IGF-2) is the dominant member of the insulin gene/protein family expressed in thymic epithelial cells (TECs) from different species, and Igf2-/- mice fail to programme complete tolerance to insulin. Based on the homology between insulin, the primary and immunogenic auto-antigen of type 1 diabetes, and IGF-2, the tolerogenic self-antigen of the insulin family, the design of a regulatory/negative self-vaccination for prevention against type 1 diabetes has been proposed and is under development. [less ▲]

Detailed reference viewed: 301 (17 ULg)
Full Text
See detailLa frontière linguistique : le mur des Belges ?
Reuchamps, Min ULg

in Département de science politique de l'Université de Liège (Ed.) Entre les murs - Un collage (2009)

This contribution, which should not be seen as a theory-driven scientifically based endeavor, raises the following question : does the linguistic border constitue the wall of the Belgians? In order to ... [more ▼]

This contribution, which should not be seen as a theory-driven scientifically based endeavor, raises the following question : does the linguistic border constitue the wall of the Belgians? In order to tackle this question, the author puts forward insights from the media, history, political science and sociology. But one should not expect to find here a solution to this tricky question… [less ▲]

Detailed reference viewed: 238 (12 ULg)
Full Text
Peer Reviewed
See detailTrophic relationships in a tropical stream food web assessed by stable isotope analysis
Coat, Sophie; Monti, Dominique; Bouchon, Claude et al

in Freshwater Biology (2009), 54(5), 1028-1041

1. Stable isotope analysis, coupled with dietary data from the literature, was used to investigate trophic patterns of freshwater fauna in a tropical stream food web (Guadeloupe, French West Indies). 2 ... [more ▼]

1. Stable isotope analysis, coupled with dietary data from the literature, was used to investigate trophic patterns of freshwater fauna in a tropical stream food web (Guadeloupe, French West Indies). 2. Primary producers (biofilm, algae and plant detritus of terrestrial origin) showed distinct delta C-13 signatures, which allowed for a powerful discrimination of carbon sources. Both autochthonous (C-13-enriched signatures) and allochthonous (C-13-depleted signatures) resources enter the food web. The migrating behaviour of fishes and shrimps between marine and freshwater during their life cycles can be followed by carbon isotopes. Here, shrimp delta C-13 signatures were shown to shift from -16 parts per thousand (for juveniles under marine influence) to -24.7 parts per thousand (for adults in freshwater habitats). For resident species, delta C-13 values partly reflected the species' habitat preferences along the river continuum: species living in river mouths were C-13-enriched in comparison with those collected upstream. 3. Nitrogen isotopic ratios were also discriminating and defined three main trophic guilds among consumers. The delta N-15 values of herbivores/detritivores were 5.0-8.4 parts per thousand, omnivores 8.8-10.2 parts per thousand and carnivores 11-12.7 parts per thousand. 4. Mixing model equations were employed to calculate the possible range of contribution made by respective food sources to the diet of each species. The results revealed the importance of omnivorous species and the dependence of riverine biota on terrestrial subsidies, such as leaf detritus and fruits. Finally, the abundance of shrimps and their feeding habits placed in relief their key role in tropical freshwater food webs. Isotopic analysis provides a useful tool for assessing animal feeding patterns. [less ▲]

Detailed reference viewed: 101 (2 ULg)
Full Text
Peer Reviewed
See detailNiche differentiation among neotropical soldierless soil-feeding termites revealed by stable isotope ratios
Bourguignon, Thomas; Sobotnik, Jan; Lepoint, Gilles ULg et al

in Soil Biology & Biochemistry (2009), 41

Termites represent one of the most abundant belowground animal taxa in tropical rainforests, where their species richness is much higher than in any other ecosystem. This high diversity in soil ecosystems ... [more ▼]

Termites represent one of the most abundant belowground animal taxa in tropical rainforests, where their species richness is much higher than in any other ecosystem. This high diversity in soil ecosystems is however difficult to explain by classical Hutchinsonian niche theory, as there is little evidence for spatial or temporal separation between species. Using δ13C and δ15N isotopic ratios, we tested if resource partitioning along the humification gradient occurs in neotropical soldierless termites of the Anoplotermes-group. Two distinct sites were investigated to check if interspecific differences are transposable between sites. Significant differences in δ15N were found between species of the Anoplotermes-group. Although some species displayed higher intersite δ15N variation than others, species-average δ15N values for both sites were highly correlated, showing that sympatric soldierless soil-feeding termites feed on distinct components of the soil. Our data also suggest that some species are more likely to shift along this gradient than others, in response to overall habitat conditions or to the presence of competitors. Feeding niche differentiation can therefore account for the high species richness and diversity of soldierless soil-feeding termites in neotropical rainforests. [less ▲]

Detailed reference viewed: 120 (3 ULg)
Full Text
Peer Reviewed
See detailWood-based diet and gut microflora of a galatheid crab associated with Pacific deep-sea wood falls
Hoyoux, Caroline ULg; Zbinden, Magali; Samadi, Sarah et al

in Marine Biology (2009)

Wood falls in the deep sea have recently become the focus of studies showing their importance as nutrients on the deep-sea Xoor. In such environments, Crustaceans constitute numerically the second-largest ... [more ▼]

Wood falls in the deep sea have recently become the focus of studies showing their importance as nutrients on the deep-sea Xoor. In such environments, Crustaceans constitute numerically the second-largest group after Mollusks. Many questions have arisen regarding their trophic role therein. A careful examination of the feeding appendages, gut contents, and gut lining of Munidopsis andamanica caught with wood falls revealed this species as a truly original detritivorous species using wood and the biofilm covering it as two main food sources. Comparing individuals from other geographic areas from substrates not reported highlights the galatheid crab as specialist of refractory substrates, especially vegetal remains. M. andamanica also exhibits a resident gut microXora consisting of bacteria and fungi possibly involved in the digestion of wood fragments. The results suggest that Crustaceans could be full-fledged actors in the food chains of sunken-wood ecosystems and that feeding habits of some squat lobsters could be different than scavenging. [less ▲]

Detailed reference viewed: 171 (12 ULg)
Full Text
Peer Reviewed
See detailBases immunologiques a la comprehension du concept d'anticorps monoclonal.
Moutschen, Michel ULg; Scheen, André ULg

in Revue Médicale de Liège (2009), 64(5-6), 237-43

Antibodies (Ab) are molecules with dual functions: on the one hand, they bind to antigens (Ag) through their variable regions (Fab, "Fragment antigen binding"), located at one of their extremities; on the ... [more ▼]

Antibodies (Ab) are molecules with dual functions: on the one hand, they bind to antigens (Ag) through their variable regions (Fab, "Fragment antigen binding"), located at one of their extremities; on the other hand, they recruit cells of the immune system, via the other extremity, the constant region or Fc region, which results in a selective destruction of cells that have the corresponding Ag. The capacity of recognition of Ag by Ab is unique. Ag generally have several different epitopes that all are binding sites for Ab. Ab may be classified according to their ability to recognize one single epitope or several epitopes. They are called monoclonal Ab (mAb) or polyclonal Ab, respectively. MAbs recognize the same epitope because they are issued from one single line of plasmocytes, originating from one single cell. It is the reason why they are so selective. MAbs exert complex, but unique mechanisms of action: they inhibit or activate signal transduction, and they specifically drive the immune system against target cells, such as tumoral cells. After their extensive use in fundamental and in applied research as wells as diagnostic tools, mAbs are now largely exploited in therapeutics. [less ▲]

Detailed reference viewed: 152 (4 ULg)
Full Text
Peer Reviewed
See detailNomenclature internationale des differents types d'anticorps monoclonaux.
Scheen, André ULg

in Revue Médicale de Liège (2009), 64(5-6), 244-7

Significant advances in the development of monoclonal antibodies ("mabs") have been acknowledged during the last two decades. Successive developments led to the marketing of murine antibodies ("o-mab ... [more ▼]

Significant advances in the development of monoclonal antibodies ("mabs") have been acknowledged during the last two decades. Successive developments led to the marketing of murine antibodies ("o-mab" first, followed by chimeric antibodies ("xi-mab"), humanised antibodies ("zu-mab") and, finally, human monoclonal antibodies ("u-mab"). In order to facilitate the distinction between the various monoclonal antibodies used in clinical practice, an international nomenclature has been proposed with the use of a specific suffix corresponding to the origine/source of "mabs" preceded by an infix referring to the medicine's target. The efforts in developing new types of monoclonal antibodies aimed at improving their pharmacokinetics (longer half-life), pharmacodynamics (better efficacy because of stronger affinity to human receptor), and safety profile (less antigenic and immunogenic reactions). These progresses could be obtained thanks to the remarkable development of molecular biotechnology. [less ▲]

Detailed reference viewed: 497 (3 ULg)
Full Text
Peer Reviewed
See detailNouvelles avancees dans l'utilisation des anticorps monoclonaux en therapeutique.
Scheen, André ULg

in Revue Médicale de Liège (2009), 64(5-6), 253-6

New major advances may be expected in a near future regarding the use of monoclonal antibodies (mAb), mainly thanks to the remarkable progress in biotechnology and the better knowledge of cellular ... [more ▼]

New major advances may be expected in a near future regarding the use of monoclonal antibodies (mAb), mainly thanks to the remarkable progress in biotechnology and the better knowledge of cellular homeostasis. The concept of antibody-targeted drugs relies upon the use of mAbs as vectors capable of specifically delivering at targeted cells (i.e. tumours) other active agents that could exert locally a cytotoxic effect (radioisotope, toxin, enzyme, drug). Other approaches will also be concidered such as the development and use of bispecific mAbs, intracellular mAbs or fragments of mAbs. These novel original approaches open promising therapeutic prospects. However, in many cases, their real interest in terms of "efficacy-safety-cost" ratio remains to be demonstrated as compared to other classical therapeutic approaches. [less ▲]

Detailed reference viewed: 289 (4 ULg)
Full Text
Peer Reviewed
See detailLes anticorps monoclonaux en thérapeutique.
Scheen, André ULg; Moutschen, Michel ULg

in Revue Médicale de Liège (2009), 64(5-6), 233-6

Detailed reference viewed: 95 (7 ULg)