Publications ORBi OA
Bookmark and Share    
Full Text
Peer Reviewed
See detailEvidence for a Role of Early Oestrogens in the Central Processing of Sexually Relevant Olfactory Cues in Female Mice
Pierman, S.; Douhard, Quentin ULg; Bakker, Julie ULg

in European Journal of Neuroscience (2008), 27(2), 423-31

We previously found that female aromatase knockout (ArKO) mice showed less investigation of socially relevant odours as well as reduced sexual behaviour. We now ask whether these behavioural deficits ... [more ▼]

We previously found that female aromatase knockout (ArKO) mice showed less investigation of socially relevant odours as well as reduced sexual behaviour. We now ask whether these behavioural deficits might be due to an inadequate processing of odours in female ArKO mice. Therefore, we exposed female ArKO mice to same- and opposite-sex urinary odours and determined the expression of the immediate early gene c-Fos along the main and accessory olfactory projection pathways. We included ArKO males in the present study as we previously observed that they show female-typical detection thresholds of urinary odours, suggesting a role for perinatal oestrogens in these behavioural responses. No sex or genotype differences were observed in the olfactory bulb after urine exposure. By contrast, sex differences in c-Fos responses were observed in wild-type (WT) mice following exposure to male urine in the more central regions of the olfactory pathway; only WT females showed a significant Fos induction in the amygdala, central medial pre-optic area and ventromedial hypothalamus. However, ArKO females did not show a c-Fos response to male odours in the ventromedial hypothalamus, suggesting that the processing of male odours is affected in ArKO females and thus that oestrogens may be necessary for the development of neural responses to sexually relevant odours in female mice. By contrast, c-Fos responses to either male or oestrous female urine were very similar between ArKO and WT males, pointing to a central role of androgen vs. oestrogen signalling in the male circuits that control olfactory investigation and preferences. [less ▲]

Detailed reference viewed: 6 (0 ULg)
Full Text
Peer Reviewed
See detail(19) F NMR in vivo spectroscopy reflects the effectiveness of perfusion - enhancing vascular modifiers for improving gemcitabine chemotheraypy
Cron, Greg O.; Ansiaux; MARTINIVE, Philippe ULg et al

in Magnetic Resonance in Medicine : Official Journal of the Society of Magnetic Resonance in Medicine (2008)

Laboratory of Biomedical Magnetic Resonance and Laboratory of Medicinal Chemistry and Radiopharmacy, Université Catholique de Louvain, UCL, Brussels, Belgium. Nuclear magnetic resonance spectroscopy of ... [more ▼]

Laboratory of Biomedical Magnetic Resonance and Laboratory of Medicinal Chemistry and Radiopharmacy, Université Catholique de Louvain, UCL, Brussels, Belgium. Nuclear magnetic resonance spectroscopy of fluorine-19 ((19)F NMR) has proven useful for evaluating kinetics of fluorinated chemotherapy drugs in tumors in vivo. This work investigated how three perfusion-enhancing vascular modifiers (BQ123, thalidomide, and Botulinum neurotoxin type A [BoNT-A]) would affect the chemotherapeutic efficacy of gemcitabine, a fluorinated drug widely used in human cancer treatment. Murine tumor growth experiments demonstrated that only BoNT-A showed a strong trend to enhance tumor growth inhibition by gemcitabine (1.7 days growth delay, P = 0.052, Student t-test). In accord with these results, (19)F NMR experiments showed that only BoNT-A increased significantly the uptake of gemcitabine in tumors (50% increase, P = 0.0008, Student t-test). Further experiments on gemcitabine kinetics (NMR vs time) and distribution ((19)F MRI) confirmed the uptake-enhancing properties of BoNT-A. The results of this study demonstrate that (19)F NMR can monitor modulation of the pharmacokinetics of fluorinated chemotherapy drugs in tumors. The results also show that (19)F NMR data can give a strong indication of the effectiveness of perfusion-enhancing vascular modifiers for improving gemcitabine chemotherapy in murine tumors. (19)F NMR is a promising tool for preclinical evaluation of such vascular modifiers and may ultimately be used in the clinic to monitor how these modifiers affect chemotherapy. 2007 Wiley-Liss, Inc PMID: 18050344 [PubMed - indexed for MEDLINE] [less ▲]

Detailed reference viewed: 65 (8 ULg)
Full Text
See detailLe pousse-pousse à gougouttes
Terwagne, Denis ULg; Eddi, Antonin; Fort, Emmanuel et al

Conference (2008, January)

Detailed reference viewed: 24 (1 ULg)
Full Text
Peer Reviewed
See detailBreeding sites of bluetongue vectors in northern Europe
Zimmer, Jean-Yves ULg; Haubruge, Eric ULg; Francis, Frédéric ULg et al

in Veterinary Record : Journal of the British Veterinary Association (2008), 162(4), 131

Detailed reference viewed: 101 (64 ULg)
Full Text
Peer Reviewed
See detailRole for Estradiol in Female-Typical Brain and Behavioral Sexual Differentiation
Bakker, Julie ULg; Baum, M. J.

in Frontiers in Neuroendocrinology (2008), 29(1), 1-16

The importance of estrogens in controlling brain and behavioral sexual differentiation in female rodents is an unresolved issue in the field of behavioral neuroendocrinology. Whereas, the current dogma ... [more ▼]

The importance of estrogens in controlling brain and behavioral sexual differentiation in female rodents is an unresolved issue in the field of behavioral neuroendocrinology. Whereas, the current dogma states that the female brain develops independently of estradiol, many studies have hinted at possible roles of estrogen in female sexual differentiation. Accordingly, it has been proposed that alpha-fetoprotein, a fetal plasma protein that binds estrogens with high affinity, has more than a neuroprotective role and specifically delivers estrogens to target brain cells to ensure female differentiation. Here, we review new results obtained in aromatase and alpha-fetoprotein knockout mice showing that estrogens can have both feminizing and defeminizing effects on the developing neural mechanisms that control sexual behavior. We propose that the defeminizing action of estradiol normally occurs prenatally in males and is avoided in fetal females because of the protective actions of alpha-fetoprotein, whereas the feminizing action of estradiol normally occurs postnatally in genetic females. [less ▲]

Detailed reference viewed: 31 (0 ULg)
Full Text
Peer Reviewed
See detailL'Image du mois. L'acide 5-Amino-Levulinique (5-ALA) dans le traitement des tumeurs cérébrales malignes
Luckers, O.; James, S.; Ghassempour, K. et al

in Revue Médicale de Liège (2008), 63(1), 4-5

Detailed reference viewed: 15 (0 ULg)
Full Text
See detailContribution à la compréhension du fonctionnement hydrogeologique du système aquifère dans le bassin du Kou (Burkina Faso)
Sauret, Elie ULg

Master of advanced studies dissertation (2008)

Located at the South of the town of Dioulasso sore, economic capital of Burkina Faso, the basin of the Kou consists of sandy, carbonated and clayed formations dated from the Precambrian one. These ... [more ▼]

Located at the South of the town of Dioulasso sore, economic capital of Burkina Faso, the basin of the Kou consists of sandy, carbonated and clayed formations dated from the Precambrian one. These formations constitute materials of four aquifers formations whose geometrical characteristics and the interactions with the rivers and the important sources of Nasso (10.000 m3/h) and Pésso (600 m3/h) remain complex and badly known. In order to provide a better comprehension of the hydrogeology of this vast basin of 1860 km2, the present study was initiated. For the needs for the study, the limits of the Kou basin were extended to its natural borders: the Mouhoun river in North-Western and the cliff of Banfora in the South-East. The four aquifers formations considered as a single hydrogeological unit, were modelled considering steady state groundwater flow conditions. Modeling made it possible to understand the interactions groundwater -water of surface by quantifying exchanged water flows. The results provided by this model appear acceptable in comparison of the allowed assumptions, and the data we have used. [less ▲]

Detailed reference viewed: 141 (8 ULg)
Full Text
Peer Reviewed
See detailSurgical correction of ischaemic mitral regurgitation - still a long way to go
Kolh, Philippe ULg

in European Heart Journal (2008), 29

Detailed reference viewed: 44 (25 ULg)
Full Text
Peer Reviewed
See detailAnalgésie par blocs nerveux périphériques continus dans le cadre des soins de plaies cutanées
Lecoq, Jean-Pierre; Jacquemin, Denise ULg; Lamy, Maurice ULg et al

in Revue Médicale de Liège (2008), 63(1), 3-16

We present the case of a patient with a circumferential venous ulcer at the level of the calf. She is hospitalized for surgical treatment by continuous aspiration dressing and coverage with skin graft ... [more ▼]

We present the case of a patient with a circumferential venous ulcer at the level of the calf. She is hospitalized for surgical treatment by continuous aspiration dressing and coverage with skin graft. This patient presents a history of chronic pain, on which classical medications have few effects and are accompanied by side effects. After discussion with the patient, a double continuous peripheral nervous block (femoral and sciatic nerve) is set up to improve the tolerance to wound dressing. This technique presents favorable antalgic results. The indications and the different techniques of block performance (by electrostimulation or echo-guided) are described. The infectious risks related to the presence of catheter near cutaneous wounds are clarified according to recent data of literature. The relevance of these techniques in correlation with the development of chronic pain and on the trophicity of the wounds are also discussed. [less ▲]

Detailed reference viewed: 115 (7 ULg)
Full Text
Peer Reviewed
See detailGastroenterology - Clinical and genetic factors associated with sacroiliitis in Crohn's disease
Peeters, Harald; Cruyssen, Bert Vander; Mielants, Herman et al

in Journal of Gastroenterology and Hepatology (2008), 23(1), 132-137

Background and Aim: Radiographic sacroiliitis (SI), often asymptomatic, is considered the most frequent extra-intestinal manifestation (EIM) of Crohn's disease (CD). Data on the association of SI with ... [more ▼]

Background and Aim: Radiographic sacroiliitis (SI), often asymptomatic, is considered the most frequent extra-intestinal manifestation (EIM) of Crohn's disease (CD). Data on the association of SI with other clinical features of CD are limited. Association of SI with CARD15 polymorphisms has recently been suggested. In a multicenter study, we investigated the association of SI in CD patients with clinical phenotypes, other EIM and CARD15 polymorphisms. Methods: Radiographs of the sacroiliac joints were taken in 251 CD patients from three Belgian university hospitals and scored by two blinded rheumatologists. Clinical features were obtained from medical records. Forty-three percent of patients carried at least one CARD15 polymorphism. Results: Sacroiliitis, defined as the presence of at least grade 2 unilateral changes, was diagnosed in 65 of the 244 scorable radiographs (27%). Only 16 of these patients were previously diagnosed with ankylosing spondylitis (AS). HLA-B27 positivity was observed in 53% of patients with AS and 7% of patients with radiographic SI. In univariate and multivariate analysis, associations between the presence of SI and peripheral arthritis (P = 0.005) and between AS and uveitis (P = 0.005) were found. No associations with other recorded clinical features or with CARD15 polymorphisms were observed. Conclusion: We confirm the high prevalence of radiographic sacroiliitis in a multicenter CD cohort. Uveitis is only associated with AS whereas all patients with SI are more prone to develop peripheral arthritis during their disease course, suggesting similar pathogenetic mechanisms in the development of these EIM. The previously reported association between SI and CARD15 polymorphisms was not confirmed. [less ▲]

Detailed reference viewed: 48 (3 ULg)
Full Text
See detailLes maladies vénériennes chez le cheval
Ponthier, Jérôme ULg; Deleuze, Stefan ULg

in sBs Magazine (2008)

Detailed reference viewed: 76 (16 ULg)
Full Text
Peer Reviewed
See detailActes du colloque 'Enseignement du lexique et des terminologies : corpus, stratégies et méthodes'
Thiry, Bernard ULg

in Cahiers de la Recherche en UER-Langues de HEC-ULg (2008), (2),

Detailed reference viewed: 43 (11 ULg)
Full Text
Peer Reviewed
See detailSoil physical fertility: thesis project for water-soil-plant model improvement
Beckers, Eléonore ULg; Degre, Aurore ULg

Poster (2008, January)

Quantities of nutrients like N, P and K in the soil are generally assumed to be the more important parameters of its fertility. The physical fertility of the soil such as water holding capacity, hydraulic ... [more ▼]

Quantities of nutrients like N, P and K in the soil are generally assumed to be the more important parameters of its fertility. The physical fertility of the soil such as water holding capacity, hydraulic conductivity…is often ignored. The present thesis project purposes the development of a physically-based model linking agricultural practices and changes on soil physical properties. [less ▲]

Detailed reference viewed: 92 (14 ULg)
Full Text
Peer Reviewed
See detailFalse positive PTH results: An easy strategy to test and detect analytical interferences in routine practice
Cavalier, Etienne ULg; Carlisi, Agnès; Chapelle, Jean-Paul ULg et al

in Clinica Chimica Acta (2008), 387(1-2), 150-152

Background: As other immunoassays, PTH determination is not free from interferences. Indeed, natural antibodies like heterophile antibodies (HAMA) and rheumatoid factor (RF) can induce falsely elevated ... [more ▼]

Background: As other immunoassays, PTH determination is not free from interferences. Indeed, natural antibodies like heterophile antibodies (HAMA) and rheumatoid factor (RF) can induce falsely elevated results, leading to misdiagnosis and expensive unnecessary explorations. However, in routine practice, these interferences are not always obvious to detect. Methods: On 2084 PTH samples, we applied a validation strategy in four steps to screen for HAMA and rheumatoid factor interferences. Results: 36% of our samples presented an elevated PTH. We found a clinically plausible reason for 91% of them. The remaining 63 suspicious samples were treated with HBT and 40% of them were found to be HAMA positive. RF determination was performed on the HAMA-negative samples and RE was positive in 21 of them. They were then treated with RF-Absorbent. Nine of these 21 samples presented RE interference. Conclusion: Applying this strategy in our routine validation, we managed to avoid spuriously elevated PTH results, which could have caused medical errors as well as unnecessary cost-effective extra-investigations. (c) 2007 Elsevier B.V. All rights reserved. [less ▲]

Detailed reference viewed: 86 (6 ULg)
Full Text
Peer Reviewed
See detailXMM-Newton X-ray study of early type stars in the Carina OB1 association
Antokhin, I. I.; Rauw, Grégor ULg; Vreux, Jean-Marie ULg et al

in Astronomy and Astrophysics (2008), 477(2), 593-609

Aims. X-ray properties of the stellar population in the Carina OB I association are examined with special emphasis on early-type stars. Their spectral characteristics provide some clues to understanding ... [more ▼]

Aims. X-ray properties of the stellar population in the Carina OB I association are examined with special emphasis on early-type stars. Their spectral characteristics provide some clues to understanding the nature of X-ray formation mechanisms in the winds of single and binary early-type stars. Methods. A timing and spectral analysis of five observations with XMM-Newton is performed using various statistical tests and thermal spectral models. Results. 235 point sources have been detected within the field of view. Several of these sources are probably pre-main sequence stars with characteristic short-term variability. Seven sources are possible background AGNs. Spectral analysis of twenty four sources of type OB and WR 25 was performed. We derived spectral parameters of the sources and their fluxes in three energy bands. Estimating the interstellar absorption for every source and the distance to the nebula, we derived X-ray luminosities of these stars and compared them to their bolometric luminosities. We discuss possible reasons for the fact that, on average, the observed X-ray properties of binary and single early type stars are not very different, and give several possible explanations. [less ▲]

Detailed reference viewed: 17 (0 ULg)
Full Text
Peer Reviewed
See detailThe combined immunodetection of AP-2alpha and YY1 transcription factors is associated with ERBB2 gene overexpression in primary breast tumors.
Allouche, Abdelkader; Nolens, Gregory ULg; Tancredi, Annalisa ULg et al

in Breast Cancer Research [=BCR] (2008), 10(1), 9

INTRODUCTION: Overexpression of the ERBB2 oncogene is observed in about 20% of human breast tumors and is the consequence of increased transcription rates frequently associated with gene amplification ... [more ▼]

INTRODUCTION: Overexpression of the ERBB2 oncogene is observed in about 20% of human breast tumors and is the consequence of increased transcription rates frequently associated with gene amplification. Several studies have shown a link between activator protein 2 (AP-2) transcription factors and ERBB2 gene expression in breast cancer cell lines. Moreover, the Yin Yang 1 (YY1) transcription factor has been shown to stimulate AP-2 transcriptional activity on the ERBB2 promoter in vitro. In this report, we examined the relationships between ERBB2, AP-2alpha, and YY1 both in breast cancer tissue specimens and in a mammary cancer cell line. METHODS: ERBB2, AP-2alpha, and YY1 protein levels were analyzed by immunohistochemistry in a panel of 55 primary breast tumors. ERBB2 gene amplification status was determined by fluorescent in situ hybridization. Correlations were evaluated by a chi2 test at a p value of less than 0.05. The functional role of AP-2alpha and YY1 on ERBB2 gene expression was analyzed by small interfering RNA (siRNA) transfection in the BT-474 mammary cancer cell line followed by real-time reverse transcription-polymerase chain reaction and Western blotting. RESULTS: We observed a statistically significant correlation between ERBB2 and AP-2alpha levels in the tumors (p < 0.01). Moreover, associations were found between ERBB2 protein level and the combined high expression of AP-2alpha and YY1 (p < 0.02) as well as between the expression of AP-2alpha and YY1 (p < 0.001). Furthermore, the levels of both AP-2alpha and YY1 proteins were inversely correlated to ERBB2 gene amplification status in the tumors (p < 0.01). Transfection of siRNAs targeting AP-2alpha and AP-2gamma mRNAs in the BT-474 breast cancer cell line repressed the expression of the endogenous ERBB2 gene at both the mRNA and protein levels. Moreover, the additional transfection of an siRNA directed against the YY1 transcript further reduced the ERBB2 protein level, suggesting that AP-2 and YY1 transcription factors cooperate to stimulate the transcription of the ERBB2 gene. CONCLUSION: This study highlights the role of both AP-2alpha and YY1 transcription factors in ERBB2 oncogene overexpression in breast tumors. Our results also suggest that high ERBB2 expression may result either from gene amplification or from increased transcription factor levels. [less ▲]

Detailed reference viewed: 141 (17 ULg)