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See detailApplication of multiple-point geostatistics on modelling groundwater flow and transport in a cross-bedded aquifer
Huysmans, Marijke; Dassargues, Alain ULg

in Atkinson, P. M. M.; Lloyd, C. D. D. (Eds.) GeoENV VII – Geostatistics for Environmental Applications, Quantitative Geology and Geostatistics (2010)

In this work, the problem of modelling groundwater flow and transport in a heterogeneous environment with complex geological structures is addressed. This study demonstrates how a training image can be ... [more ▼]

In this work, the problem of modelling groundwater flow and transport in a heterogeneous environment with complex geological structures is addressed. This study demonstrates how a training image can be constructed based on geological and hydrogeological field data and how multiple-point geostatistics can be applied to determine the impact of complex geological heterogeneity on groundwater flow and transport in a real aquifer. [less ▲]

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See detailLe bananier: comment obtenir du matériel de plantation indemne de virus
De Clerck, Caroline ULg; Lassois, Ludivine ULg

Diverse speeche and writing (2010)

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See detailL'assainissement viral des bananiers: une étape primordiale dans la préservation de la biodiveristé
Lassois, Ludivine ULg; Castaigne, Michel; D'haes, François

Diverse speeche and writing (2010)

Le laboratoire de Phytopathologie de Gembloux Agro-Bio Tech a développé les techniques permettant la détection et l’assainissement des plantules de bananiers virosés. Ce laboratoire travaille en étroite ... [more ▼]

Le laboratoire de Phytopathologie de Gembloux Agro-Bio Tech a développé les techniques permettant la détection et l’assainissement des plantules de bananiers virosés. Ce laboratoire travaille en étroite collaboration avec Bioversity International afin de fournir au Centre de Transit International, et à fortiori à la communauté internationale, des bananiers indemnes de virus. Les bananiers ainsi obtenus peuvent donc être diffusés largement sans craintes d’une épidémie quelconque. Ce travail d’assainissement contribue non seulement à la sauvegarde de la biodiversité mais également à sa valorisation en rendant saines des variétés qui étaient virosées et non exploitables. Jusqu’à présent l’ITC a fourni 15 000 plantules saines de bananiers dans 335 régions réparties dans 100 pays. Ce travail conjoint de conservation et d’assainissement permet de valoriser la grande diversité génétique du bananier au service de la communauté internationale. [less ▲]

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See detailRole of delta-like-4/Notch in the formation and wiring of the lymphatic network in zebrafish.
Geudens, I.; Herpers, R.; Hermans, K. et al

in Arteriosclerosis, Thrombosis, and Vascular Biology (2010), 30(9), 1695-702

OBJECTIVE: To study whether Notch signaling, which regulates cell fate decisions and vessel morphogenesis, controls lymphatic development. METHODS AND RESULTS: In zebrafish embryos, sprouts from the axial ... [more ▼]

OBJECTIVE: To study whether Notch signaling, which regulates cell fate decisions and vessel morphogenesis, controls lymphatic development. METHODS AND RESULTS: In zebrafish embryos, sprouts from the axial vein have lymphangiogenic potential because they give rise to the first lymphatics. Knockdown of delta-like-4 (Dll4) or its receptors Notch-1b or Notch-6 in zebrafish impaired lymphangiogenesis. Dll4/Notch silencing reduced the number of sprouts producing the string of parchordal lymphangioblasts; instead, sprouts connecting to the intersomitic vessels were formed. At a later phase, Notch silencing impaired navigation of lymphatic intersomitic vessels along their arterial templates. CONCLUSIONS: These studies imply critical roles for Notch signaling in the formation and wiring of the lymphatic network. [less ▲]

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See detailHistological and transcriptional study of angiogenesis and lymphangiogenesis in uninvolved skin, acute pinpoint lesions and established psoriasis plaques: an approach of vascular development chronology in psoriasis
Henno, Audrey ULg; Blacher, Silvia ULg; Lambert, Charles ULg et al

in Journal of Dermatological Science (2010), 57(3), 162-169

Background Dysregulation of angiogenesis and lymphangiogenesis could participate in psoriasis pathogenesis. Analysis of nascent psoriasis lesions should help at identifying early vascular anomalies ... [more ▼]

Background Dysregulation of angiogenesis and lymphangiogenesis could participate in psoriasis pathogenesis. Analysis of nascent psoriasis lesions should help at identifying early vascular anomalies. Objective To analyse vascular development, angiogenesis and lymphangiogenesis markers expression in uninvolved skin in psoriatic patients (N), early psoriasis lesions or pinpoints (PP) and psoriasis plaques (PSO). Methods Skin biopsies were taken in 17 patients in N and in PSO and/or PP. The mRNA steady-state level of angiogenesis and lymphangiogenesis markers was measured by RT-PCR. Immunohistochemistry was performed for von Willebrand factor, podoplanin, Ki-67 and VEGFR3. Blood (BV) and lymphatic (LV) vessels expansion was measured by computer-assisted morphometry. Results Clinical and epidermal aspects indicated that PP are intermediate between N and PSO. While total BV area was already increased in PP similarly to PSO as compared to N, LV area in PP was intermediate between N and PSO. Mean LV size was identical in N and PP and increased in PSO, mean BV size in PP being intermediate between N and PSO. VEGF-A 189 variant was increased in PP as compared to N and PSO. As compared to N, angiogenesis markers (VEGF-A isoforms, PlGF, VEGFR2, NRP-1), VEGF-C and NRP-2 were similarly increased in PP and PSO. Keratin 16 and the lymphangiogenesis markers (VEGFR3, prox-1) were intermediate in PP. Conclusion These data suggest that the expansion of lymphatic vessels occurs after blood vascular development in psoriasis. Expansion of BV in PP could be followed by vessel enlargement during progression to PSO, in parallel with a decreased VEGF-A 189/VEGF-A 121 balance in plaques [less ▲]

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See detailTIMP-2 binding with cellular MT1-MMP stimulates invasion-promoting MEK/ERK signaling in cancer cells
Sounni, Nor Eddine ULg; Rozanov, D. V.; Remacle, A. G. et al

in International Journal of Cancer = Journal International du Cancer (2010), 126(5), 1067-78

Both invasion-promoting MT1-MMP and its physiological inhibitorTIMP-2 play a significant role in tumorigenesis and are identified in the most aggressive cancers. Despite its antiproteolytic effects in ... [more ▼]

Both invasion-promoting MT1-MMP and its physiological inhibitorTIMP-2 play a significant role in tumorigenesis and are identified in the most aggressive cancers. Despite its antiproteolytic effects in vitro, clinical data suggest that TIMP-2 expression is positively associated with tumor recurrence, thus emphasizing the wide-ranging role of TIMP-2 in malignancies. To shed light on this role of TIMP-2, we report that low concentrations of TIMP-2, by interacting with MT1-MMP (a specific membrane receptor of TIMP-2), induce the MEK/ERK signaling cascade in fibrosarcoma HT1080 cells which express MT1-MMP naturally. TIMP-2 binding with cell surface-associated MT1-MMP stimulates phosphorylation of MEK1/2, which is upstream of ERK1/2, and the ERK1/2 substrate p90RSK. Consistent with volumes of literature, we confirmed that the activation of ERK stimulated cell migration. Both the transcriptional silencing of MT1-MMP and the inhibition of MEK1/2 reversed the signaling effects of TIMP-2/MT1-MMP while the active site-targeting MMP inhibitor GM6001 did not. Our data suggest that both the interactions of TIMP-2 with MT1-MMP, which activate the pro-migratory ERK signaling cascade, and the conventional inhibition of MT1-MMP's catalytic activity by TIMP-2, play a role in the invasion-promoting function of MT1-MMP. The TIMP-2-induced stimulation of ERK signaling in cancer cells explains the direct, as opposed to the inverse, association of TIMP-2 expression with poor prognosis in cancer. [less ▲]

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See detailLa banane: de la récolte à la consommation
De Clerck, Caroline ULg; Lassois, Ludivine ULg

Diverse speeche and writing (2010)

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See detaille bananier: de la plantation à la récolte
De Clerck, Caroline ULg; Lassois, Ludivine ULg

Diverse speeche and writing (2010)

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See detailL'exraction par micro-ondes: un choix de qualité
Ramdani, M.; Ghazi, Z.; Fadel, O. et al

Poster (2010)

Le but de ce travail est de comparer l’extraction faite par chauffage classique (CC) avec celle réalisée sous micro-ondes (MO). Deux modes d’extraction ont été réalisés : - L’hydro-distillation par ... [more ▼]

Le but de ce travail est de comparer l’extraction faite par chauffage classique (CC) avec celle réalisée sous micro-ondes (MO). Deux modes d’extraction ont été réalisés : - L’hydro-distillation par chauffage classique (extraction par entraînement à la vapeur d’un solvant) et, - L’hydro-distillation activée par micro-ondes. Les MO permettent un gain de temps considérable. Par exemple, dans le cas du lavandula dentata : on note une cinétique 3 fois plus rapide, de plus le rendement est nettement supérieur (1% contre 0,6% par chauffage classique). Dans le cas de la plante fraiche, l’extraction est faite en absence de solvant sous MO. Ceci présente un grand intérêt sur le plan économique et environnemental. De plus, la qualité de l’huile extraite présente généralement une meilleure pureté. L’extraction par MO constitue une technique en accord avec la chimie verte. Grâce à ce procédé, on pourra alors réduire d'environ 60 % les besoins en énergie, ce qui diminuera d'autant les émissions de gaz à effet de serre. [less ▲]

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See detailCyclodimerization by ring-closing metathesis: synthesis, computational and biological evaluation of novel bis-azetidinyl-macrocycles
Sliwa, Aline; Dive, Georges ULg; Habib Jiwan, Jean-Louis et al

in Tetrahedron (2010), 66

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See detailReview of three Recent Books on the Boundary of Bioinformatics and Systems Biology
Bullinger, Eric ULg; Schliemann, Monica ULg

in BioMedical Engineering OnLine (2010), 9(1), 33

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See detailStochastic analysis of a stadium roof from deterministic wind tunnel measurements
Blaise, Nicolas ULg; Denoël, Vincent ULg

in 13th International Conference on Wind Engineering (2010)

Dynamic analyses of structures under buffeting wind loads can be performed in a deterministic (Clough and Penzien, 1997) or stochastic (Preumont, 1994) context, both with a modal approach for ... [more ▼]

Dynamic analyses of structures under buffeting wind loads can be performed in a deterministic (Clough and Penzien, 1997) or stochastic (Preumont, 1994) context, both with a modal approach for computational efficiency reasons. In the first option, the forces are deterministically given, and the uncoupled modal equations of motion are solved either in the time domain with a stepby- step method, either in the frequency domain, with Fourier transformation. In the second option, the analysis relies on the determination of the Power Spectral Density (PSD) matrix of the structural response given that of the loading. The choice of one or another method usually depends on whether the loading is provided in the time or frequency domain and as a deterministic (a single time history) or stochastic manner. From a designer’s point of view, the wind loading can be defined using design codes (e.g. Eurocode, 2005) where analytical expressions of (i) the PSD of wind velocities (Davenport, Von Karman, etc) (ii) the coherence functions and (iii) the pressure coefficients are given to compute, finally, (iv) the PSD of the aerodynamic pressures. Design engineers are usually familiar with this probabilistic approach. Alternatively, the design may be conducted from aerodynamic pressures measured in a wind tunnel. This approach is more realistic than the aforementioned codified procedure since a number of phenomena as (a) the aerodynamic instabilities, (b) aerodynamic admittance (Scanlan and Jones, 1999), (c) site effects are taken into account. Pressures are thus given as unique (deterministic) time histories at each sensor. In a Finite Element context and a modal analysis, the generalized forces are computed from the measured pressures. With the firm wish to perform the analysis in a stochastic manner (for a number of good reasons mentioned next), we suggest to fit a probabilistic model to the measured data. Such a model could be fitted to the measured pressures right away, or any other subsequent quantity such as the generalized forces. The following discussion is about the most favorable quantity that has to be fitted and how to do it appropriately in view of typical measurement imperfections. [less ▲]

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See detailShort communication: Genetic relationship between calving traits and body condition score before and after calving in Canadian Ayrshire second-parity cows.
Bastin, Catherine ULg; Loker, Sarah; Gengler, Nicolas ULg et al

in Journal of Dairy Science (2010), 93(9), 4398-403

The objective of this study was to investigate the genetic relationship between body condition score (BCS) and calving traits (including calving ease and calf survival) for Ayrshire second-parity cows in ... [more ▼]

The objective of this study was to investigate the genetic relationship between body condition score (BCS) and calving traits (including calving ease and calf survival) for Ayrshire second-parity cows in Canada. The use of random regression models allowed assessment of the change of genetic correlation from 100 d before calving to 335 d after calving. Therefore, the influence of BCS in the dry period on subsequent calving could be studied. Body condition scores were collected by field staff several times over the lactation in 101 herds from Quebec and calving records were extracted from the official database used for Canadian genetic evaluation of calving ease. Daily heritability of BCS increased from 0.07 on d 100 before calving to 0.25 at 335 d in milk. Genetic correlations between BCS at different stages ranged between 0.59 and 0.99 and indicated that genetic components for BCS did not change much over lactation. With the exception of the genetic correlation between BCS and direct calving ease, which was low and negative, genetic correlations between BCS and calving traits were positive and moderate to high. Correlations were the highest before calving and decreased toward the end of the ensuing lactation. The correlation between BCS 10 d before calving and maternal calving ease was 0.32 and emphasized the relationship between fat cows before calving with dystocia. Standards errors of the genetic correlations estimates were low. Genetic correlations between BCS and calf survival were moderate to high and favorable. This indicates that cows with a genetically high BCS across lactation would have a greater chance of producing a calf that survived (maternal calf survival) and that they would transmit genes that allow the calf to survive (direct calf survival). [less ▲]

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See detailMembrane type 1 matrix metalloproteinase detection in tumors, using the iodinated endogenous [123I]-tissue inhibitor 2 of metalloproteinases as imaging agent.
Van Steenkiste, Magali; Oltenfreiter, Ruth; Frankenne, Francis et al

in Cancer Biotherapy & Radiopharmaceuticals (2010), 25(5), 511-20

Matrix metalloproteinases (MMPs) are principal participants in tumor development. In addition to serve as a useful biochemical marker, MMP expression may also provide a target for the diagnostic in vivo ... [more ▼]

Matrix metalloproteinases (MMPs) are principal participants in tumor development. In addition to serve as a useful biochemical marker, MMP expression may also provide a target for the diagnostic in vivo imaging of tumors, using a radiolabeled inhibitor. This study investigates the use of membrane type 1 (MT1)-MMP as target for in vivo tumor diagnosis. Specific binding of the endogenous tissue inhibitor of metalloproteinase-2 (TIMP-2) to MT1-MMP has been previously described. In this study, biodistribution and imaging experiments were performed on MT1-MMP-overexpressing (S.1.5) and control (C.IV.3) tumor-inoculated mice using [(123)I]-recombinant human TIMP-2 (rhTIMP-2) as radioligand and [(123)I]-rhTIMP-1 as control. The expression profile was controlled in vitro and on tumor extracts. rhTIMP-2 as well as rhTIMP-1 were labeled using the Iodogen method and characterized. Biodistribution of [(123)I]-rhTIMP-2 showed a tumor uptake of 2.87% +/- 1.58% ID/g at 3 hours postinjection in S.1.5. Tumor values of [(123)I]-rhTIMP-1 and [(123)I]-rhTIMP-2 evaluated in S.1.5 and C.IV.3, respectively, were significantly lower. Planar imaging revealed significant uptake of [(123)I]-rhTIMP-2 in S.1.5 compared with contralateral background areas. This could not be observed in C.IV.3 and with [(123)I]-rhTIMP-1 in S.1.5. All tumors were well established (200-800 mg). These results suggest that rhTIMP-2 holds potential for development of radiotracers for in vivo imaging in overexpressing MT1-MMP but not in similar tumors that do not express this protease. [less ▲]

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See detailDe internationalisering van de Vlaamse strip
Meesters, Gert ULg

in Praagse Perspectieven (2010), 6

De traditionele Vlaamse strip is ongewoon populair in de eigen regio, maar heeft — op Suske en Wiske en enkele andere uitzonderingen na — in het buitenland nooit een grote bekendheid verkregen. Sinds ... [more ▼]

De traditionele Vlaamse strip is ongewoon populair in de eigen regio, maar heeft — op Suske en Wiske en enkele andere uitzonderingen na — in het buitenland nooit een grote bekendheid verkregen. Sinds enkele jaren is het Vlaamse striplandschap echter grondig aan het veranderen. Jonge auteurs zetten een heel ander soort strips op de kaart en krijgen daarvoor zowel in eigen regio als ver daarbuiten erkenning. In deze lezing zal ik die nieuwe beweging in zijn context plaatsen en enkele boeken gedetailleerder bespreken, zoals De maagd en de neger van Judith Vanistendael en Over naar jou van Philip Paquet en Adriaan Van Aken. [less ▲]

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See detailTove Jansson's Dreamworld
Meesters, Gert ULg

in International Journal of Comic Art [=IJOCA] (2010), 12(2-3), 648-649

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See detailNotariat et courtage immobilier. Soleil à l'horizon
Kohl, Benoît ULg

in Journal des Tribunaux (2010)

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See detailDetermination of a Suitable Protocol for Indigenous Oilseed Cucurbits Plant Regeneration
Gnamien, Yah Gwladys; Zoro Bi, I. A.; Djè, Y. et al

in Tropicultura (2010), 28(4), 217-225

The present work was carried out to establish rapid in vitro propagation of the indigenous oilseed Citrullus lanatus. Efficiency of 3 protocols for seed decontamination was investigated. High level of ... [more ▼]

The present work was carried out to establish rapid in vitro propagation of the indigenous oilseed Citrullus lanatus. Efficiency of 3 protocols for seed decontamination was investigated. High level of seed sterilization was obtained after removing seed coat and soaking the seeds in 1.6 % sodium hypochlorite with a drop of Tween 20. Shoot tips, single node and cotyledon explants of 3 morphotypes have been screened for adventitious shoot formation in tissue culture. Best response in termis of multiple shoot induction was obtained from cotyledon proximal part with hypocotyl segmenet on Murashige and Skoog medium supplemented with 1 mg/l 6-Benzylaminopurine, 30 g/l sucrose and 8 g/l agar. After 3 weeks of culture, 90% of cotyledon proximal parts induced shoot. An average of 12.6 shoots per explants and a mean shoot length of 8 mm were obtained after multiplication stage. Shoot induction appeared to be strongly influenced by genotype and explant type. The percentage of shoot induction from cotyledon proximal parts ranged from 23.3% to 64.0% according to the genotype. Efficient rooting was achieved on half-solid MS medium containing 0.1 mg/l 1-Naphthaleneactic acid or without growth regulator. Acclimatised plantlets were transferred to greenhouse where they grew and flowered like seeded plantlets. [less ▲]

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See detailDistribution of EFHC1 or myoclonin 1 in mouse neural structures
Leon, Christine ULg; de Nijs, Laurence ULg; Chanas, Grazyna et al

in Epilepsy Research (2010), 88

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See detailLa fonction rénale
Cavalier, Etienne ULg

Learning material (2010)

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