Publications ORBi OA
Bookmark and Share    
Full Text
Peer Reviewed
See detailEffect of non-steroidal anti-inflammatory drugs on amyloid-beta formation and macrophage activation after platelet phagocytosis.
Jans, Dominique ULg; Martinet, Wim; Fillet, Marianne ULg et al

in Journal of Cardiovascular Pharmacology (2004), 43(3), 462-70

Recently, we showed that platelet phagocytosis occurs in human atherosclerotic plaques and leads to foam cell formation. Platelet phagocytosis, resulting in macrophage activation and iNOS induction, was ... [more ▼]

Recently, we showed that platelet phagocytosis occurs in human atherosclerotic plaques and leads to foam cell formation. Platelet phagocytosis, resulting in macrophage activation and iNOS induction, was associated with the formation of amyloid-beta peptide (Abeta) via proteolytic cleavage of platelet-derived amyloid precursor protein (APP), possibly by secretases. To test the involvement of gamma-secretase in this process, we used indomethacin, ibuprofen, and sulindac sulfide, non-steroidal anti-inflammatory drugs (NSAIDs) known to alter the gamma-secretase cleaving site of APP, on their ability to inhibit macrophage activation evoked by platelet phagocytosis. J774 macrophages were incubated with human platelets or lipopolysaccharide (LPS) with or without NSAIDs. Nitrite was quantified as a measure for inducible nitric oxide synthase (iNOS) activity. Indomethacin, ibuprofen, sulindac sulfide, and meloxicam concentration-dependently reduced nitrite production by macrophages incubated with platelets, but did not alter LPS-induced iNOS activity or platelet uptake. However, acetylsalicylic acid and naproxen, two NSAIDs without effect on the gamma-secretase cleaving site of APP, did not affect nitrite production in either platelet- or LPS-stimulated macrophages. Surface-enhanced laser desorption/ionization time-of-flight mass-spectrometry demonstrated time-dependent formation of Abeta-containing peptides after platelet phagocytosis, which could be inhibited by indomethacin. In conclusion, these results point to the involvement of gamma-secretase in macrophage activation following platelet phagocytosis. [less ▲]

Detailed reference viewed: 18 (2 ULg)
Full Text
See detailFrasnian carbonate mounds of the Frasnes area, Belgium
Boulvain, Frédéric ULg

in IAS Newsletters (2004), 191

Detailed reference viewed: 12 (3 ULg)
Full Text
Peer Reviewed
See detailATP Augments von Willebrand Factor-dependent Shear-induced Platelet Aggregation through Ca2+-Calmodulin and Myosin Light Chain Kinase Activation
Oury, Cécile ULg; Sticker, Elsie; Cornelissen, Heidi et al

in Journal of Biological Chemistry (2004)

Shear stress triggers von Willebrand factor (VWF) binding to platelet glycoprotein Ibalpha and subsequent integrin alpha(IIb)beta(3)-dependent platelet aggregation. Concomitantly, nucleotides are released ... [more ▼]

Shear stress triggers von Willebrand factor (VWF) binding to platelet glycoprotein Ibalpha and subsequent integrin alpha(IIb)beta(3)-dependent platelet aggregation. Concomitantly, nucleotides are released from plateletdense granules, and ADP is known to contribute to shear-induced platelet aggregation (SIPA). This study shows that ATP also contributes to SIPA. The ATP-gated P2X(1) ion channel induces MLC-mediated cytoskeletal rearrangements that increases platelet degranulation during VWF-triggered platelet activation. [less ▲]

Detailed reference viewed: 19 (4 ULg)
Full Text
See detailValorisation industrielle de la pomme de terre.
Delaplace, Pierre ULg; Fauconnier, Marie-Laure ULg

in Troupeaux et Cultures des Tropiques (2004)

Detailed reference viewed: 72 (10 ULg)
Full Text
See detailAN INTRODUCTION TO ROBOTICS: MECHANICAL ASPECTS
Géradin, Michel ULg; Duysinx, Pierre ULg

Learning material (2004)

Detailed reference viewed: 178 (9 ULg)
Full Text
See detailLeçon inaugurale : entre mythes et défis, la forêt en devenir
Rondeux, Jacques ULg

in Bulletin d'Information (Association Royale des Ingénieurs Issus de la Fusagx - Aigx) (2004), (5), 9-15

Detailed reference viewed: 21 (3 ULg)
Full Text
See detailLa forêt privée wallonne et sa gestion : des chiffres qui parlent
Colson, Vincent ULg; Puissant, T.; Hebert, Jacques ULg et al

Book (2004)

An investigation relative to Walloon private forest owners was carried out in order to characterise their properties and the management undergone in each one of them, as well as to identify the main ... [more ▼]

An investigation relative to Walloon private forest owners was carried out in order to characterise their properties and the management undergone in each one of them, as well as to identify the main problems the owners encounter. The results of this study emphasise on the existence of multiple aspects of owners and of their properties. The majority of the Walloon private properties represent above all an important inheritance along with a strong sentimental attachment in regards to the owner. A high proportion of owners manage their forest themselves by devoting a lot of time, but without necessarily planning management. This study clearly shows that a large number of owners wish to be more informed and guided. [less ▲]

Detailed reference viewed: 107 (15 ULg)
Full Text
See detailInventaire forestier de gestion : proposition d'un cadre méthodologique et technique
Lejeune, Philippe ULg; Rondeux, Jacques ULg

in Forêt Wallonne (2004), (73), 22-29

Confronté à des sollicitations de plus en plus nombreuses envers la forêt, le gestionnaire forestier doit pouvoir disposer d’informations adaptées à la complexité des analyses qu’il est amené à réaliser ... [more ▼]

Confronté à des sollicitations de plus en plus nombreuses envers la forêt, le gestionnaire forestier doit pouvoir disposer d’informations adaptées à la complexité des analyses qu’il est amené à réaliser. Les progrès récents en matière de cartographie donnent accès à une information très précieuse. L’inventaire forestier reste cependant l’outil le plus adapté pour une description, tant qualitative que quantitative, des peuplements et de leurs diverses composantes. Ces inventaires ne peuvent cependant plus se limiter au simple recensement du matériel sur pied présent au sein des différentes parcelles d’une propriété. [less ▲]

Detailed reference viewed: 70 (16 ULg)
Full Text
Peer Reviewed
See detailDouces transgressions : notes sur "La Perle" (H. d'Ursel, 1929)
Belloi, Livio ULg

in Mélusine. Cahiers du Centre de Recherche sur le Surréalisme (2004), 26

Cette étude consiste en une lecture approfondie et contextualisante d’un film belge peu connu, à savoir "La Perle" (Henri d’Ursel, 1929, sur un scénario du poète Georges Hugnet). De cet examen ... [more ▼]

Cette étude consiste en une lecture approfondie et contextualisante d’un film belge peu connu, à savoir "La Perle" (Henri d’Ursel, 1929, sur un scénario du poète Georges Hugnet). De cet examen circonstancié et spécialement attentif aux phénomènes d’intertextualité, il ressort notamment que "La Perle" tranche à bien des égards avec les autres grandes œuvres cinématographiques habituellement placées sous la bannière du surréalisme (les films de Bunuel et Dali, de Germaine Dulac, de Man Ray, etc.). En particulier, le film d’Henri d’Ursel se règle sur le principe d’une transgression douce, observant apparemment les conventions narratives, mais dans le seul but de mieux les miner de l’intérieur. Rien de très spectaculaire dans "La Perle", sinon l’expression même des tiraillements intimes dont l’esthétique surréaliste se trouve affectée, entre culture populaire (référence aux "Vampires", le célèbre film à épisodes de Louis Feuillade) et raréfaction poétique (allusion à Stéphane Mallarmé et à son "Coup de dés"). [less ▲]

Detailed reference viewed: 213 (16 ULg)
Full Text
Peer Reviewed
See detailInnovative artificial fog production device - A technical facility for research activities.
Colomb, M.; Dufour, J.; Hirech, M. et al

Conference (2004)

A new innovative fog production device has been developed in close cooperation with partners of a European project called “FOG” funded by the EU. It is set up in the fog chamber of the road and bridges ... [more ▼]

A new innovative fog production device has been developed in close cooperation with partners of a European project called “FOG” funded by the EU. It is set up in the fog chamber of the road and bridges laboratory at Clermont-Ferrand. Most of the activities of this laboratory are dedicated to road safety but the facility is open to any other activity, such as environmental research. The recent developments consist in setting up a controllable device able to produce stable visibility levels and homogeneous fog, representative of various types of natural water droplet distribution. The fog characteristics were determined and compared to natural fog. Results are presented for a selection of conditions including stabilized visibility levels for dense fog, less than 50 metres meteorological visibility and two kinds of droplet distribution. [less ▲]

Detailed reference viewed: 38 (3 ULg)
Full Text
Peer Reviewed
See detailCancer du pancréas et vertèbre d'ivoire
Guillaume, Thierry ULg; Krzesinski, Jean-Marie ULg; Lambinet, Nadine ULg

in Revue Médicale de Liège (2004), 59(12), 685-687

En résumé, nous avons donc objectivé un adénocarcinome de la tête pancréatique cliniquement asymptomatique suite à la mise en évidence d’une lésion osseuse métastatique condensante douloureuse. Le cas ... [more ▼]

En résumé, nous avons donc objectivé un adénocarcinome de la tête pancréatique cliniquement asymptomatique suite à la mise en évidence d’une lésion osseuse métastatique condensante douloureuse. Le cas d’un adénocarcinome pancréatique révélé par une métastase osseuse surtout condensante, étant relativement rare, nous a paru intéressant à rapporter. [less ▲]

Detailed reference viewed: 210 (3 ULg)
Full Text
Peer Reviewed
See detailMossbauer spectral study of the magnetocaloric FeMnP1-xAsx compounds
Hermann, Raphaël ULg; Tegus, O.; Bruck, E. et al

in Physical Review b (2004), 70(21),

The magnetic phase transitions in the FeMnP1-xAsx compounds with x= 0.25, 0.35, 0.45, 0.50, and 0.55, have been studied by iron-57 Mossbauer spectroscopy. The ferromagnetic and antiferromagnetic spectra ... [more ▼]

The magnetic phase transitions in the FeMnP1-xAsx compounds with x= 0.25, 0.35, 0.45, 0.50, and 0.55, have been studied by iron-57 Mossbauer spectroscopy. The ferromagnetic and antiferromagnetic spectra have been analyzed with a model that takes into account the random distribution of the P and As near-neighbor anions of a given iron site. This distribution is a binomial distribution of the contributions to the spectra of each iron with n As near neighbors. A magnetostriction model has been used to characterize the temperature induced paramagnetic to ferromagnetic first-order phase transition and order parameters, eta(F)=2.6, 2.3, 2.0, 1.57, and 1.43 have been obtained for x=0.25, 0.35, 0.45, 0.50, and 0.55, respectively. A detailed phase diagram has been derived from the Mossbauer spectral analysis and reveals a magnetic triple point at x=similar to0.35 and similar to210 K. A model that takes into account the random binomial P and As distribution and the contribution from the iron and manganese magnetic sublattices yields excellent fits of the spectral components assigned to the ferromagnetic and incommensurate antiferromagnetic components for the x=0.25 and 0.35 compounds at all temperatures. [less ▲]

Detailed reference viewed: 38 (2 ULg)
Full Text
See detailL’ironie dans Ounamon : les emplois de mk et de ptr
Winand, Jean ULg

in Göttinger Miszellen (2004), (200), 105-110

Detailed reference viewed: 83 (3 ULg)
Full Text
Peer Reviewed
See detailIdentification of residues within human glycoprotein VI involved in the binding to collagen: evidence for the existence of distinct binding sites.
Lecut, Christelle ULg; Arocas, Veronique; Ulrichts, Hans et al

in Journal of Biological Chemistry (2004), 279(50), 52293-9

Glycoprotein VI (GPVI) has a crucial role in platelet responses to collagen. Still, little is known about its interaction with its ligands. In binding assays using soluble or cell-expressed human GPVI, we ... [more ▼]

Glycoprotein VI (GPVI) has a crucial role in platelet responses to collagen. Still, little is known about its interaction with its ligands. In binding assays using soluble or cell-expressed human GPVI, we observed that (i) collagen, and the GPVI-specific ligands collagen-related peptides (CRP) and convulxin, competed with one another for the binding to GPVI and (ii) monoclonal antibodies directed against the extracellular part of the human receptor displayed selective inhibitory properties on GPVI interaction with its ligands. Monoclonal antibody 9E18 strongly reduced the binding of GPVI to collagen/CRP, 3F8 inhibited its interaction with convulxin, whereas 9O12 prevented all three interactions. These observations suggest that ligand-binding sites are distinct, exhibiting specific features but at the same time also sharing some common residues participating in the recognition of these ligands. The epitope of 9O12 was mapped by phage display, along with molecular modeling of human GPVI, which allowed the identification of residues within GPVI potentially involved in ligand recognition. Site-directed mutagenesis revealed that valine 34 and leucine 36 are critical for GPVI interaction with collagen and CRP. The loop might thus be part of a collagen/CRP-binding site. [less ▲]

Detailed reference viewed: 15 (6 ULg)
Full Text
Peer Reviewed
See detailPrincipal role of glycoprotein VI in alpha2beta1 and alphaIIbbeta3 activation during collagen-induced thrombus formation.
Lecut, Christelle ULg; Schoolmeester, Anne; Kuijpers, Marijke J E et al

in Arteriosclerosis, Thrombosis, and Vascular Biology (2004), 24(9), 1727-33

OBJECTIVE: High-shear perfusion of blood over collagen results in rapid platelet adhesion, aggregation, and procoagulant activity. We studied regulation of alpha2beta1 and alphaIIbbeta3 integrin ... [more ▼]

OBJECTIVE: High-shear perfusion of blood over collagen results in rapid platelet adhesion, aggregation, and procoagulant activity. We studied regulation of alpha2beta1 and alphaIIbbeta3 integrin activation during thrombus formation on collagen. METHODS AND RESULTS: Blockade of glycoprotein (GP) VI by 9O12 antibody or of P2Y purinergic receptors permitted platelet adhesion but reduced aggregate formation, fibrinogen binding, and activation of alpha2beta1 and alphaIIbbeta3, as detected with antibodies IAC-1 and PAC1 directed against activation-dependent epitopes of these integrins. Combined blockade of GPVI and P2Y receptors and thromboxane formation abolished integrin activation but still allowed adhesion of morphologically unstimulated, nonprocoagulant platelets. Exogenous ADP partly restored the suppressive effect of GPVI blockade on integrin alpha2beta1 and alphaIIbbeta3 activation. Adhesion was fully inhibited only with simultaneous blocking of GPVI and alpha2beta1, indicating that the integrin can support platelet-collagen binding in the absence of its activation. Blockade or absence of GPIbalpha only moderately influenced integrin activation and adhesion unless GPVI was inhibited. CONCLUSIONS: GPVI- and autocrine-released ADP induce affinity changes of alpha2beta1 and alphaIIbbeta3 during thrombus formation on collagen under flow. These integrin changes are dispensable for adhesion but strengthen platelet-collagen interactions and thereby collagen-induced platelet activation. Integrin activation during thrombus formation on collagen was studied using fluorescent-labeled antibodies IAC-1 and PAC1 directed against activation-dependent epitopes of alpha2beta1 and alphaIIbbeta3 integrin, respectively. Glycoprotein VI blockade by 9O12 antibody or P2Y ADP receptors reduced integrin activation along with aggregate formation and fibrinogen binding but not alpha2beta1-dependent adhesion. [less ▲]

Detailed reference viewed: 29 (2 ULg)
Full Text
Peer Reviewed
See detailL’application de la loi dans le temps en matière de roulage
Jacobs, Ann ULg

in Revue de Jurisprudence de Liège, Mons et Bruxelles (2004)

Detailed reference viewed: 67 (0 ULg)
Full Text
Peer Reviewed
See detailLa peine subsidiaire à une peine de travail
Jacobs, Ann ULg

in Revue de Droit Pénal et de Criminologie (2004)

Detailed reference viewed: 60 (4 ULg)
Full Text
Peer Reviewed
See detailPerquisitions et droits de la défense : une remise en question des pratiques par la Cour européenne des droits de l’homme ?
Jacobs, Ann ULg

in Revue de Droit Pénal et de Criminologie (2004)

Confrontation de la jurisprudence belge en matière de perquisitions à la jurisprudence de la Cour européenne des droits de l'homme, en particulier sous l'angle des mentions du mandat de perquisition et du ... [more ▼]

Confrontation de la jurisprudence belge en matière de perquisitions à la jurisprudence de la Cour européenne des droits de l'homme, en particulier sous l'angle des mentions du mandat de perquisition et du déroulement de la perquisition. [less ▲]

Detailed reference viewed: 147 (2 ULg)
Full Text
See detailNouvelles approches du diagnostic et de la pathogénie du diabète de type 1
Geenen, Vincent ULg

in Louvain Medical (2004), 123

Detailed reference viewed: 17 (0 ULg)
Full Text
Peer Reviewed
See detailParametric accelerated failure time models with random effects and an application to kidney transplant survival
Lambert, Philippe ULg; Collett, Dave; Kimber, Alan et al

in Statistics in Medicine (2004), 23

Accelerated failure time models with a shared random component are described, and are used to evaluate the effect of explanatory factors and different transplant centres on survival times following kidney ... [more ▼]

Accelerated failure time models with a shared random component are described, and are used to evaluate the effect of explanatory factors and different transplant centres on survival times following kidney transplantation. Different combinations of the distribution of the random effects and baseline hazard function are considered and the fit of such models to the transplant data is critically assessed. A mixture model that combines short- and long-term components of a hazard function is then developed, which provides a more flexible model for the hazard function. The model can incorporate different explanatory variables and random effects in each component. The model is straightforward to fit using standard statistical software, and is shown to be a good fit to the transplant data. [less ▲]

Detailed reference viewed: 41 (3 ULg)