Publications ORBi OA
Bookmark and Share    
Full Text
Peer Reviewed
See detailNew integrative method to generate Bacillus subtilis recombinant strains free of selection markers
Brans, Alain ULg; Filée, Patrice ULg; Chevigné, Andy ULg et al

in Applied and Environmental Microbiology (2004), 70(12), 7241-7250

The novel method described in this paper combines the use of blaI, which encodes a repressor involved in Bacillus licheniformis BlaP beta-lactamase regulation, an antibiotic resistance gene, and a B ... [more ▼]

The novel method described in this paper combines the use of blaI, which encodes a repressor involved in Bacillus licheniformis BlaP beta-lactamase regulation, an antibiotic resistance gene, and a B. subtilis strain (BS1541) that is conditionally auxotrophic for lysine. We constructed a BlaI cassette containing blaI and the spectinomycin resistance genes and two short direct repeat DNA sequences, one at each extremity of the cassette. The BS1541 strain was obtained by replacing the B. subtilis P(lysA) promoter with that of the P(blaP) beta-lactamase promoter. In the resulting strain, the cloning of the blaI repressor gene confers lysine auxotrophy to BS1541. After integration of the BlaI cassette into the chromosome of a conditionally lys-auxotrophic (BS1541) strain by homologous recombination and positive selection for spectinomycin resistance, the eviction of the BlaI cassette was achieved by single crossover between the two short direct repeat sequences. This strategy was successfully used to inactivate a single gene and to introduce a gene of interest in the Bacillus chromosome. In both cases the resulting strains are free of selection marker. This allows the use of the BlaI cassette to repeatedly further modify the Bacillus chromosome. [less ▲]

Detailed reference viewed: 54 (8 ULg)
Full Text
See detailLa poésie irlandaise aujourd'hui : Michael et Seamus, Medbh et Caitriona
Pagnoulle, Christine ULg

Scientific conference (2004, December)

Detailed reference viewed: 18 (6 ULg)
Full Text
See detailLa sexualité du couple vieillissant : quelle distribution des rôles ?
Kempeneers, Philippe ULg

in Patient Care, Neuropsychiatrie (2004), 4

Detailed reference viewed: 52 (5 ULg)
Full Text
Peer Reviewed
See detailAn insulin-like growth factor 2-derived self-antigen inducing a regulatory cytokine profile after presentation to peripheral blood mononuclear cells from DQ8(+) type 1 diabetic adolescents - Preliminary design of a thymus-based tolerogenic self-vaccination
Geenen, Vincent ULg; Louis, Céline ULg; Martens, Henri ULg

in Annals of the New York Academy of Sciences (2004), 1037

This work aims to evaluate the potential use of insulin-like growth factor 2 (IGF-2) as the dominant thymic self-antigen precursor of the insulin family in designing a tolerogenic approach to type 1 ... [more ▼]

This work aims to evaluate the potential use of insulin-like growth factor 2 (IGF-2) as the dominant thymic self-antigen precursor of the insulin family in designing a tolerogenic approach to type 1 diabetes (T1D) prevention. This evaluation was primarily based on cytokine profile driven by MHC presentation of insulin and IGF-2-derived antigens to PBMC cultures derived from 16 T1D DQ8(+) adolescents. Insulin B9-23, one dominant P-cell autoantigen, and the homologous sequence B11-25 of IGF-2 display the same affinity and fully compete for binding to DQ8, a MHC-II allele conferring major genetic susceptibility to type 1 diabetes (T1D). However, compared to insulin beta 9-23, presentation of IGF-2 B11-25 elicits a suppressive/regulatory cytokine profile with a higher number of IL-10-secreting cells (P < 0.05), a much higher ratio of IL-10/IFN-gamma (P < 0.01), as well as a lower number of IL-4-secreting cells (P < 0.05). Thus, with regard to T1D prevention, administration of IGF-2-derived self-antigen(s) seems to be an efficient approach that combines both antagonism for binding to a major susceptibility MHC-II allele, as well as downstream promotion of an antigen-driven tolerogenic response. [less ▲]

Detailed reference viewed: 24 (0 ULg)
Full Text
Peer Reviewed
See detailApplications of C and N stable isotopes to ecological and environmental studies in seagrass ecosystems
Lepoint, Gilles ULg; Dauby, Patrick ULg; Gobert, Sylvie ULg

in Marine Pollution Bulletin (2004), 49(11-12), 887-891

Stable isotopes of carbon and nitrogen are increasingly used in marine ecosystems, for ecological and environmental studies. Here, we examine some applications of stable isotopes as ecological integrators ... [more ▼]

Stable isotopes of carbon and nitrogen are increasingly used in marine ecosystems, for ecological and environmental studies. Here, we examine some applications of stable isotopes as ecological integrators or tracers in seagrass ecosystem studies. We focus on both the use of natural isotope abundance as food web integrators or environmental tracers and on the use of stable isotopes as experimental tools. As ecosystem integrators, stable isotopes have helped to elucidate the general structure of trophic webs in temperate, Mediterranean and tropical seagrass ecosystems. As environmental tracers, stable isotopes have proven their utility in sewage impact measuring and mapping. However, to make such environmental studies more comprehensible, future works on understanding of basic reasons for variations of N and C stable isotopes in seagrasses should be encouraged. At least, as experimental tracers, stable isotopes allow the study of many aspects of N and C cycles at the scale of a plant or at the scale of the seagrass ecosystem. (C) 2004 Elsevier Ltd. All rights reserved. [less ▲]

Detailed reference viewed: 49 (9 ULg)
Full Text
Peer Reviewed
See detailDevelopment of a universal microarray based on the ligation detection reaction and 16S rRNA gene polymorphism to target diversity of cyanobacteria
Castiglioni, Bianca; Rizzi, Ermann; Frosini, Andrea et al

in Applied and Environmental Microbiology (2004), 70(12), 7161-7172

The cyanobacteria are photosynthetic prokaryotes of significant ecological and biotechnological interest, since they strongly contribute to primary production and are a rich source of bioactive compounds ... [more ▼]

The cyanobacteria are photosynthetic prokaryotes of significant ecological and biotechnological interest, since they strongly contribute to primary production and are a rich source of bioactive compounds. In eutrophic fresh and brackish waters, their mass occurrences (water blooms) are often toxic and constitute a high potential risk for human health. Therefore, rapid and reliable identification of cyanobacterial species in complex environmental samples is important. Here we describe the development and validation of a microarray for the identification of cyanobacteria in aquatic environments. Our approach is based on the use of a ligation detection reaction coupled to a universal array. Probes were designed for detecting 19 cyanobacterial groups including Anabaena/Aphanizomenon, Calothrix, Cylindrospermopsis, Cylindrospermum, Gloeothece, halotolerants, Leptolyngbya, Palau Lyngbya, Microcystis, Nodularia, Nostoc, Planktothrix, Antarctic Phormidium, Prochlorococcus, Spirulina, Synechococcus, Synechocystis, Trichodesmium, and Woronichinia. These groups were identified based on an alignment of over 300 cyanobacterial 16S rRNA sequences. For validation of the microarrays, 95 samples (24 axenic strains from culture collections, 27 isolated strains, and 44 cloned fragments recovered from environmental samples) were tested. The results demonstrated a high discriminative power and sensitivity to 1 fmol of the PCR-amplified 16S rRNA gene. Accurate identification of target strains was also achieved with unbalanced mixes of PCR amplicons from different cyanobacteria and an environmental sample. Our universal array method shows great potential for rapid and reliable identification of cyanobacteria. It can be easily adapted to future development and could thus be applied both in research and environmental monitoring. [less ▲]

Detailed reference viewed: 44 (10 ULg)
Full Text
Peer Reviewed
See detailHard X-ray emission clumps in the gamma-Cygni supernova remnant: An INTEGRAL-ISGRI view
Bykov, A. M.; Krassilchtchikov, A. M.; Uvarov, Y. A. et al

in Astronomy and Astrophysics (2004), 427(3), 21-24

Spatially resolved images of the galactic supernova remnant G78.2+2.1 (gamma-Cygni) in hard X-ray energy bands from 25 keV to 120 keV are obtained with the IBIS-ISGRI imager aboard the International Gamma ... [more ▼]

Spatially resolved images of the galactic supernova remnant G78.2+2.1 (gamma-Cygni) in hard X-ray energy bands from 25 keV to 120 keV are obtained with the IBIS-ISGRI imager aboard the International Gamma-Ray Astrophysics Laboratory INTEGRAL. The images are dominated by localized clumps of about ten arcmin in size. The flux of the most prominent North-Western (NW) clump is (1.7+/-0.4)x10(-11) erg cm(-2) s(-1) in the 25-40 keV band. The observed X-ray fluxes are in agreement with extrapolations of soft X-ray imaging observations of gamma-Cyg by ASCA GIS and spatially unresolved RXTE PCA data. The positions of the hard X-ray clumps correlate with bright patches of optical line emission, possibly indicating the presence of radiative shock waves in a shocked cloud. The observed spatial structure and spectra are consistent with model predictions of hard X-ray emission from nonthermal electrons accelerated by a radiative shock in a supernova interacting with an interstellar cloud, but the powerful stellar wind of the O9V star HD 193322 is a plausible candidate for the NW source as well. [less ▲]

Detailed reference viewed: 30 (0 ULg)
Full Text
Peer Reviewed
See detailOxytocin- and vasopressin-induced growth of human small-cell lung cancer is mediated by the mitogen-activated protein kinase pathway
Pequeux, Christel ULg; Keegan, B. P.; Hagelstein, M. T. et al

in Endocrine-Related Cancer (2004), 11(4), 871-885

Malignant growth of small-cell lung carcinoma is promoted by various neuroendocrine autocrine/paracrine loops. Therefore, to interfere with this mitogenic process, it is crucial to elucidate the ... [more ▼]

Malignant growth of small-cell lung carcinoma is promoted by various neuroendocrine autocrine/paracrine loops. Therefore, to interfere with this mitogenic process, it is crucial to elucidate the mechanisms involved. It is known that the oxytocin (OT) and vasopressin (VP) genes, normally transcriptionally restricted in their expression, are activated in small-cell lung cancer (SCLC), concomitantly with expression of their receptors (OTR, V1aR, V1bR/V3R and V2R). The aim of the present study was to characterize, in concentrations close to physiological and pharmacological conditions, intracellular signalling events triggered by OT and VP binding to their specific receptors in SCLC cells and to identify factors mediating OT- and VP-induced mitogenic effects on SCLC. Known agonists for OTR ([Thr(4),GlY(7)]OT) and V1aR (F180), in addition to OT and VP, were able to elicit increases in cytosolic Ca2+ levels and this effect could be blocked using an OTR antagonist (OVTA) or a V1aR antagonist (SR49059) respectively. There was no activation of the cAMP pathway detected after VP, dDAVP (a V2R agonist), or OT treatment. Stimulation of SCLC cells with OT and VP led to an increase of extracellular signal-regulated kinase (ERK) 1/2 phosphorylation, maximal at 5 min, and the subsequent phosphorylation of its downstream target p90 ribosomal S6 kinase (p90(RSK)). Pre-incubation with OVTA and SR49059, and with inhibitors of phospholipase C (PLC), protein kinase C (PKC), mitogen-activated protein kinase/ERK kinase (MEK) 1/2 and a Ca2+ chelator significantly reduced OT- and VP-induced ERK1/2 phosphorylations. OVTA, SR49059 as well as MEK1/2 and PKC inhibitors also downregulated OT- and VP-induced p90RSK phosphorylation. In [H-3]thymidine-uptake 2, experiments, we subsequently observed that PLC, Ca2+, PKC and ERK1/2 are absolutely required for the OT- and VP-stimulated SCLC cellular growth process. In conclusion, the results presented here indicate that OT- and VP-induced mitogenic effects on SCLC are respectively mediated by OTR and V1aR signalling and that this mitogenic signalling passes through the phosphorylation of ERK1/2 and p90(RSK) in a PLC-, Ca2+-, PKC- and MEK1/2-dependent pathway. [less ▲]

Detailed reference viewed: 21 (2 ULg)
Full Text
Peer Reviewed
See detailPhenomenal characteristics associated with projecting oneself back into the past and forward into the future: Influence of valence and temporal distance
D'Argembeau, Arnaud ULg; Van der Linden, Martial ULg

in Consciousness & Cognition (2004), 13(4), 844-858

As humans, we frequently engage in mental time travel, reliving past experiences and imagining possible future events. This study examined whether similar factors affect the subjective experience ... [more ▼]

As humans, we frequently engage in mental time travel, reliving past experiences and imagining possible future events. This study examined whether similar factors affect the subjective experience associated with remembering the past and imagining the future. Participants mentally "re-experienced" or "pre-experienced" positive and negative events that differed in their temporal distance from the present (close versus distant), and then rated the phenomenal characteristics (i.e., sensorial, contextual, and emotional details) associated with their representations. For both past and future, representations of positive events were associated with a greater feeling of re-experiencing (or pre-experiencing) than representations of negative events. In addition, representations of temporally close events (both past and future) contained more sensorial and contextual details, and generated a stronger feeling of re-experiencing (or pre-experiencing) than representations of temporally distant events. It is suggested that the way we both remember our past and imagine our future is constrained by our current goals. (C) 2004 Elsevier Inc. All rights reserved. [less ▲]

Detailed reference viewed: 83 (3 ULg)
Full Text
Peer Reviewed
See detailProtein phosphorylation as a key mechanism for the regulation of BCL-3 activity
Viatour, Patrick ULg; Merville, Marie-Paule ULg; Bours, Vincent ULg et al

in Cell Cycle (Georgetown, Tex.) (2004), 3(12), 1498-1501

Constitutive NF-kappaB activation, a hallmark of many human cancers, upregulates anti-apoptotic gene expression and therefore disrupts the balance between apoptosis and proliferation. In some lymphomas ... [more ▼]

Constitutive NF-kappaB activation, a hallmark of many human cancers, upregulates anti-apoptotic gene expression and therefore disrupts the balance between apoptosis and proliferation. In some lymphomas, this constitutive NF-kappaB activity is the result of point mutations or translocations of the genes coding for NF-kappaB inhibitors, namely IkappaBalpha or p100. The BCL-3 protein is another member of the IkappaB family and is overexpressed in a subset of human B-cell chronic lymphocytic leukemias because of a chromosomal translocation. This oncoprotein is phosphorylated by multiple kinases including GSK3 and this phosphorylation regulates BCL-3 function by modulating its oncogenic potential and by regulating the expression of a subset of its target genes. Therefore, deciphering the NF-kappaB/IkappaB protein phosphorylations is critical in order to better understand the molecular mechanisms of NF-kappaB-mediated oncogenesis. [less ▲]

Detailed reference viewed: 24 (1 ULg)
Full Text
Peer Reviewed
See detailUnusual thermoelectric behavior of packed crystalline granular metals
Ausloos, Marcel ULg; Pekala, Marek; Latuch, J. et al

in Journal of Applied Physics (2004), 96(12), 7338-7345

Loosely packed granular materials are intensively studied nowadays. Electrical and thermal transport properties should reflect the granular structure, as well as intrinsic properties. We have compacted ... [more ▼]

Loosely packed granular materials are intensively studied nowadays. Electrical and thermal transport properties should reflect the granular structure, as well as intrinsic properties. We have compacted crystalline CaAl-based metallic grains and studied the electrical resistivity and the thermoelectric power as a function of temperature (T) from 15 to 300 K. Both properties show three regimes as a function of temperature. It should be pointed out: (i) The electrical resistivity continuously decreases between 15 and 235 K, (ii) with various dependencies, e.g., similar ~ T(-3/4) at low T, while (iii) the thermoelectric power (TEP) is positive, (iv) shows a bump near 60 K, and (v) presents a rather unusual square root of temperature dependence at low temperature. It is argued that these three regimes indicate a competition between geometric and thermal processes-for which a theory seems to be missing in the case of TEP. The microchemical analysis results are also reported, indicating a complex microstructure inherent to the phase diagram peritectic intricacies of this binary alloy. (C) 2004 American Institute of Physics. [less ▲]

Detailed reference viewed: 48 (10 ULg)
Full Text
Peer Reviewed
See detailLine profile variability in the spectra of Oef stars - II. HD192281, HD14442 and HD14434
De Becker, Michaël ULg; Rauw, Grégor ULg

in Astronomy and Astrophysics (2004), 427(3), 995-1008

We present the very first analysis of the spectroscopic variability of the three rapidly rotating Oef stars HD 192281 (O5(ef)), HD14442 (O5.5ef) and HD 14434 (O6.5( ef)). Radial velocities of the He II ... [more ▼]

We present the very first analysis of the spectroscopic variability of the three rapidly rotating Oef stars HD 192281 (O5(ef)), HD14442 (O5.5ef) and HD 14434 (O6.5( ef)). Radial velocities of the He II lambda 4541 line reveal no evidence of binarity on time scales of a few days, or from one year to the next, for any of the targets. The He II lambda 4686 double-peaked emission and, to some extent, the H absorption line display significant profile variability in the spectra of all three stars. Data gathered during different observing runs spread over six years reveal a rather stable time scale for HD 192281 and HD14442, whereas the variability pattern changes significantly from one year to the other. The case of HD 14434 is less clear as no obvious time scale emerges from our analysis. In a tentative way to interpret this variability, stellar rotation remains a possible clock for HD 192281 and HD14442. However, currently available models addressing stellar rotation fail to explain some crucial aspects of the observed variability behaviour, which appear to be even more complex in the case of HD 14434. [less ▲]

Detailed reference viewed: 18 (3 ULg)
Full Text
Peer Reviewed
See detailStudy on the relationship between lineaments and borehole specific capacity in a fractured and karstified limestone area in Vietnam
Tam, V. T.; De Smedt, F.; Batelaan, Okke et al

in Hydrogeology Journal (2004), 12(6), 662-673

For a karstified limestone area in NW Vietnam, the relationship between the distribution of lineaments and borehole specific capacity is determined, resulting in the conclusion that not only the borehole ... [more ▼]

For a karstified limestone area in NW Vietnam, the relationship between the distribution of lineaments and borehole specific capacity is determined, resulting in the conclusion that not only the borehole geomorphological-hydrogeological position but also the lineament distribution influences the specific capacity. No significant spatial well yield patterns are evident in this highly fractured-karstified region. The supposition is that lineaments caused by geotectonic activities affect the local variability in borehole specific capacity. Sixteen pumping tests in conjunction with a comprehensive lineament analysis are used to prove this relationship. The boreholes and lineaments are classified into two groups according to their similarity in geomorphological-hydrogeological features. Lineaments tend to be less detectable in discharge areas (lowland, wide and flat valleys) in contrast to the high density in recharge areas (highland narrow-mountainous ravines). In addition, the presence of a stream network in the former can act as a recharge source to the underlain karstic groundwater system. Consequently, boreholes that are in the discharge areas with a lower density of lineaments often produce high yield. For recharge areas with a high density of lineaments, a good correlation is found between lineament density and borehole specific capacity. [less ▲]

Detailed reference viewed: 65 (6 ULg)
Full Text
See detailUn homme, une vocation
Claustriaux, Jean-Jacques ULg; Nuyt, Cécile

in Echosud (2004), 5

Quelles sont les raisons pour lesquelles un universitaire s'intéresse aux pays du Sud ?

Detailed reference viewed: 9 (2 ULg)
Full Text
Peer Reviewed
See detailIntérêt des modèles des plus proches voisins pour le contrôle de l'hétérogénéité spatiale : application à un essai de provenances de pin d'Alep (pinus halepensis mill.) en Tunisie
Sghaier, T.; Claustriaux, Jean-Jacques ULg; Beji, A.

in Revue de l'I.N.A.T. (2004), 19(2), 5-22

To study the importance of the spatial analysis in the control of soil heterogeneity in experiment on field, three approaches of statistical analysis were applied to a total height of trees measured at 29 ... [more ▼]

To study the importance of the spatial analysis in the control of soil heterogeneity in experiment on field, three approaches of statistical analysis were applied to a total height of trees measured at 29 years of age in a comparative test of thirty origins of Aleppo pine (Pinus halepensis Mill.), autochtonous and introduced in Tunisia. Analysis without control of heterogeneity (totally random), with a global control of heterogeneity (complete blocks) and with a local control of heterogeneity (nearest neighbour models) were used. For this last approach using to estimate the plot fertility the one of the nearby plots, various combinations of neighbour were tested with one or two coefficients of regression. The obtained results showed that the reduction of the residual mean square by comparison to the analysis without control of heterogeneity is 30 % to the complete blocks analysis and varied between 66 and 79 % for spatial analysis. Significant positive correlations between plots residues and the average of the residues of the nearby plots were observed before adjustment of data. After adjustment of data by the nearest neighbour models, these correlations were registered a reduction from 92 to 95 % according to the used model. Among the six nearest neighbour models studied, the model bringing in eight neighbours with two coefficients of regression showed itself the most effective. It also seemed that after adjustment of data by nearest neighbour models, using the structure of blocks does not produce a reduction of the residual mean square. [less ▲]

Detailed reference viewed: 73 (3 ULg)
Full Text
Peer Reviewed
See detailVirologic therapy response significantly correlates with the number of active drugs as evaluated using a LiPA HIV-1 resistance scoring system
Ziermann, Rainer; Celis, Linda; Derdelinckx, Inge et al

in Journal of Clinical Virology (2004), 31(Suppl. 1), 7-15

Background: Resistance testing is increasingly accepted as a tool in guiding the selection of human immunodeficiency virus type 1 (HIV-1) antiretroviral therapy in HIV-1 infected individuals who fail ... [more ▼]

Background: Resistance testing is increasingly accepted as a tool in guiding the selection of human immunodeficiency virus type 1 (HIV-1) antiretroviral therapy in HIV-1 infected individuals who fail their current regimen. Objectives: To descriptively compare the correlation between virologic treatment response and results using three genotypic HIV-1 drug resistance interpretation systems: the VERSANT(R) HIV-1 Resistance Assay (LiPA) system and two sequence-based interpretation systems. Study design: Specimens from 213 HIV-1-infected subjects, either starting (n = 104) or switching to (n = 109) a regimen of three or four antiretroviral drugs, were collected retrospectively at baseline and after 3 months of uninterrupted therapy. The correlation between viral load change and the number of predicted active drugs in the treatment regimen was assessed. An interpretation algorithm was recently developed to process VERSANT(R) HIV-1 Resistance Assay (LiPA) data. The number of active drugs predicted using this algorithm was rank correlated with the viral load change over a 3-month treatment period. For comparison, a similar calculation was made using two sequence-based algorithms (REGA version 5.5 and VGI GuideLines(TM) Rules 4.0), both applied on the same sequences. Results: Statistically significant (p < 0.05) correlation coefficients for each of the three HIV-1 drug resistance interpretation systems were observed in the treatment-experienced subjects on a 3-drug regimen (-0.39, -0.38, and -0.42, respectively) as well as on a 4-drug regimen (-0.33, -0.31, and -0.37, respectively). However, no significant correlation was observed in treatment-naive subjects, probably due to the very low frequency of drug resistance in these subjects. Conclusion: All three genotypic drug resistance interpretation systems (LiPA version 1, REGA version 5.5, and VGI GuideLines(TM) Rules 4.0) were statistically significantly correlated with virologic therapy response as measured by viral load testing. (C) 2004 Elsevier B.V. All rights reserved. [less ▲]

Detailed reference viewed: 23 (3 ULg)
Full Text
Peer Reviewed
See detailIncreased expression of receptor activator of NF-kappa B ligand (RANKL), its receptor RANK and its decoy receptor osteoprotegerin in the colon of Crohn's disease patients
Franchimont, N.; Reenaers, Catherine ULg; Lambert, Chantal ULg et al

in Clinical & Experimental Immunology (2004), 138(3), 491-498

Crohn's disease (CD) is associated with low bone mass due to chronic inflammation and other factors. Receptor activator of NF-kappaB ligand (RANKL), its receptor RANK and its decoy receptor ... [more ▼]

Crohn's disease (CD) is associated with low bone mass due to chronic inflammation and other factors. Receptor activator of NF-kappaB ligand (RANKL), its receptor RANK and its decoy receptor osteoprotegerin (OPG) are potentially involved in this process as they regulate osteoclastogenesis and are influenced by pro-inflammatory cytokines. The aim of this study was to determine the levels of soluble RANKL (sRANKL), RANK and OPG expression both in the serum and in the colon of CD patients. Levels of sRANKL and OPG were assessed in the serum and the supernatants of cultured colonic biopsies in patients with CD and controls by ELISA. RANK expression was explored by immunostaining and immunofluorescence of fixed colonic samples. OPG and sRANKL levels were higher in the serum of CD patients as compared to age- and sex-matched controls. Levels of sRANKL and OPG were significantly enhanced in cultured colonic biopsies from CD, and OPG levels correlated with histological inflammation, and pro- and anti-inflammatory cytokine levels. No significant correlation was found for sRANKL. RANK(+) cells were increased in the colon of CD, particularly in inflamed areas. These cells were positive for CD68 or S100 protein. We conclude that serum and local levels of sRANKL and OPG are increased in CD. Moreover, RANK is expressed in the colonic mucosa by subpopulations of activated macrophages or dendritic cells at higher levels in CD compared to normal colon. [less ▲]

Detailed reference viewed: 22 (2 ULg)
Full Text
Peer Reviewed
See detailEffect of transplantation on Posidonia oceanica shoots
Vangeluwe, Denis; Lepoint, Gilles ULg; Bouquegneau, Jean-Marie ULg et al

in Vie et Milieu-Life and Environment (2004), 54(4), 223-230

Posidonia oceanica shoots sampled from the healthy meadow in the bay of Revelatta (Corsica, France) were transplanted on metallic grids at 10 m depth. Transplanted shoots have been regularly monitored by ... [more ▼]

Posidonia oceanica shoots sampled from the healthy meadow in the bay of Revelatta (Corsica, France) were transplanted on metallic grids at 10 m depth. Transplanted shoots have been regularly monitored by the measurement of growth, biomass and C, N and P contents in the leaves and the belowground parts. After six months of experiment, the survival rates varied from 14 to 84 % on the different transplantation sites. Leaf biomass on the transplanted cuttings was lower than biomass in the control shoots but root biomass raised from December 2000 to May 2001 on the transplanted shoots. Transplant growth (8,3 +/- 2,3 mm.d(-1).shoot(-1)) after six months was similar to control growth (8,2 +/- 2,8 mm.d(-1). shoot(-1)). The C, N and P contents in transplants were significatively lower than in control shoots suggesting that transplanted cuttings are unable to meet their nutrient requirements. The modifications of the C, N, P contents in the belowground parts of the transplanted shoots indicate a mobilization of these nutrients in those compartments to produce new roots and restore a normal nutrients absorption. [less ▲]

Detailed reference viewed: 88 (3 ULg)
Full Text
Peer Reviewed
See detailL'image du mois. Ulcere perineal chez une patiente atteinte de spina bifida
Lorquet, Sophie ULg; Conti, V.; Nervo, Patricia ULg et al

in Revue Médicale de Liège (2004), 59(12), 688-90

Detailed reference viewed: 66 (4 ULg)