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See detailMise au point d'une hyperferritinémie
Delwaide, Jean ULg; Giet, Didier ULg; Lamproye, Anne ULg et al

in Revue Médicale de Liège (2006), 61(5-6), 329-333

Hemochromatosis is the most common genetic disorder in persons of northern European descent, and the majority of cases are caused by a mutation in the gene HFE. Genetic testing for hemochromatosis is ... [more ▼]

Hemochromatosis is the most common genetic disorder in persons of northern European descent, and the majority of cases are caused by a mutation in the gene HFE. Genetic testing for hemochromatosis is therefore indicated in all patients with increases in transferrine saturation and ferritin levels. When this genetic testing does not demonstrate a hemochromatosis, other diseases responsible for elevated ferritin levels have to be ruled out, mainly hemolytic anemia, chronic inflammatory disorders, liver diseases such as hepatitis B or C, alcohol abuse, and non alcoholic fatty liver disease. In demonstrated iron overload with absence of classic causes, second-line genetic testing should be considered. [less ▲]

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See detailLa résolution de problèmes : analyse des pratiques de classe au cycle 5-8
Fagnant, Annick ULg; Hindryckx, Geneviève ULg

in Actes du 4e congrès des chercheurs en éducation : le point sur la recherche en éducation en Communauté française (2006)

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See detailCombination therapy versus monotherapy: a randomised pilot study on the evolution of inflammatory parameters after ventilator associated pneumonia
Damas, Pierre ULg; Garweg, Christophe ULg; Monchi, Mehran et al

in Critical Care (2006), 10(2), 52

Introduction Combination antibiotic therapy for ventilator associated pneumonia (VAP) is often used to broaden the spectrum of activity of empirical treatment. The relevance of such synergy is commonly ... [more ▼]

Introduction Combination antibiotic therapy for ventilator associated pneumonia (VAP) is often used to broaden the spectrum of activity of empirical treatment. The relevance of such synergy is commonly supposed but poorly supported. The aim of the present study was to compare the clinical outcome and the course of biological variables in patients treated for a VAP, using a monotherapy with a beta-lactam versus a combination therapy. Methods Patients with VAP were prospectively randomised to receive either cefepime alone or cefepime in association with amikacin or levofloxacin. Clinical and inflammatory parameters were measured on the day of inclusion and thereafter. Results Seventy-four mechanically ventilated patients meeting clinical criteria for VAP were enrolled in the study. VAP was microbiologically confirmed in 59 patients (84%). Patients were randomised to receive cefepime (C group, 20 patients), cefepime with amikacin (C-A group, 19 patients) or cefepime with levofloxacin (C-L group, 20 patients). No significant difference was observed regarding the time course of temperature, leukocytosis or C-reactive protein level. There were no differences between length of stay in the intensive care unit after infection, nor in ventilator free days within 28 days after infection. No difference in mortality was observed. Conclusion Antibiotic combination using a fourth generation cephalosporin with either an aminoside or a fluoroquinolone is not associated with a clinical or biological benefit when compared to cephalosporin monotherapy against common susceptible pathogens causing VAP. [less ▲]

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See detailNo accelerated rate of protein evolution in male-biased Drosophila pseudoobscura genes.
Metta, Muralidhar ULg; Gudavalli, Rambabu; Gibert, Jean-Michel et al

in Genetics (2006), 174(1), 411-20

Sexually dimorphic traits are often subject to diversifying selection. Genes with a male-biased gene expression also are probably affected by sexual selection and have a high rate of protein evolution. We ... [more ▼]

Sexually dimorphic traits are often subject to diversifying selection. Genes with a male-biased gene expression also are probably affected by sexual selection and have a high rate of protein evolution. We used SAGE to measure sex-biased gene expression in Drosophila pseudoobscura. Consistent with previous results from D. melanogaster, a larger number of genes were male biased (402 genes) than female biased (138 genes). About 34% of the genes changed the sex-related expression pattern between D. melanogaster and D. pseudoobscura. Combining gene expression with protein divergence between both species, we observed a striking difference in the rate of evolution for genes with a male-biased gene expression in one species only. Contrary to expectations, D. pseudoobscura genes in this category showed no accelerated rate of protein evolution, while D. melanogaster genes did. If sexual selection is driving molecular evolution of male-biased genes, our data imply a radically different selection regime in D. pseudoobscura. [less ▲]

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See detailQuantitative ultrasonography for the noninvasive assessment of equine bone: a review
Carstanjen, B.; Gabriel, Annick ULg

in IPPOLOGIA (2006), 17(4), 11-15

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See detailMorphometric study of the equine fetlock and coffin joints
Riccio, Barbara; Carstanjen, Bianca; Denoix, Jean-Marie et al

in Italian Journal of Anatomy and Embryology (2006), 111

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See detailEpidémiosurveillance des événements rares chez les bovins en Belgique
Saegerman, Claude ULg

in Annales de Médecine Vétérinaire (2006), 150(Sp. Iss. SI), 4-9

Epidémiosurveillance des événements rares chez les bovins en Belgique. Thèse de doctorat en sciences vétérinaires défendue le 29 septembre 2004.

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See detailTranscriptome analysis reveals link between proteasomal and mitochondrial pathways in Parkinson's disease.
Duke, D. C.; Moran, L. B.; Kalaitzakis, M. E. et al

in Neurogenetics (2006), 7(3), 139-48

There is growing evidence that dysfunction of the mitochondrial respiratory chain and failure of the cellular protein degradation machinery, specifically the ubiquitin-proteasome system, play an important ... [more ▼]

There is growing evidence that dysfunction of the mitochondrial respiratory chain and failure of the cellular protein degradation machinery, specifically the ubiquitin-proteasome system, play an important role in the pathogenesis of Parkinson's disease. We now show that the corresponding pathways of these two systems are linked at the transcriptomic level in Parkinsonian substantia nigra. We examined gene expression in medial and lateral substantia nigra (SN) as well as in frontal cortex using whole genome DNA oligonucleotide microarrays. In this study, we use a hypothesis-driven approach in analysing microarray data to describe the expression of mitochondrial and ubiquitin-proteasomal system (UPS) genes in Parkinson's disease (PD). Although a number of genes showed up-regulation, we found an overall decrease in expression affecting the majority of mitochondrial and UPS sequences. The down-regulated genes include genes that encode subunits of complex I and the Parkinson's-disease-linked UCHL1. The observed changes in expression were very similar for both medial and lateral SN and also affected the PD cerebral cortex. As revealed by "gene shaving" clustering analysis, there was a very significant correlation between the transcriptomic profiles of both systems including in control brains. Therefore, the mitochondria and the proteasome form a higher-order gene regulatory network that is severely perturbed in Parkinson's disease. Our quantitative results also suggest that Parkinson's disease is a disease of more than one cell class, i.e. that it goes beyond the catecholaminergic neuron and involves glia as well. [less ▲]

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See detailComparative study of commercially available anti-alpha-synuclein antibodies.
Croisier, E.; MRes, D Elfant; Deprez, Manuel ULg et al

in Neuropathology & Applied Neurobiology (2006), 32(3), 351-6

Immunohistochemistry for alpha-synuclein has become the histological technique of choice for the diagnosis for Parkinson's disease, Dementia with Lewy bodies and Multiple System Atrophy (http://www.ICDNS ... [more ▼]

Immunohistochemistry for alpha-synuclein has become the histological technique of choice for the diagnosis for Parkinson's disease, Dementia with Lewy bodies and Multiple System Atrophy (http://www.ICDNS.org). Nevertheless, no standardised protocol has been proposed. We have reviewed 242 of the 270 studies published until June 2005 that mentioned immunohistochemistry for anti-alpha synuclein on human tissue and we found that only 75 (31%) used commercial antibodies. We also noted that protocols, particularly dilution and antigen unmasking, varied between studies, even when the same antibody was employed. In order to establish a standardised protocol for alpha-synuclein immunohistochemistry, which can be applied in diagnostic neuropathology we tested seven commercial monoclonal antibodies in brains of subjects with Parkinson's disease, dementia with Lewy bodies, multiple system atrophy, multiple sclerosis with incidental Lewy bodies and aged-matched normal brain and determined for each antibody the best suited protocol for antigen unmasking. We evaluated the intensity of immunolabelling in Lewy bodies, neuropil threads, dendrites, pre-synaptic terminals, granular cytoplasmic positivity, peri-axonal positivity, glial inclusions and non-specific immunolabelling. Although our results showed that all the antibodies detected alpha-synuclein inclusions, differences were noted between antibodies, particularly with regard to the detection of glial inclusions. From our study, the best antibodies of the seven tested appeared to be those directed against amino acids 116-131 and 15-123 and we suggest them to be used in routine diagnostic practice for alpha-synucleinopathies. [less ▲]

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See detailLes méthodes diagnostiques en dentisterie restauratrice et prophylactique: Evolution des outils de détection des lésions carieuses pour un diagnostic précoce
Gueders, Audrey ULg; Geerts, Sabine ULg

in Journal du dentiste (2006), 338

La mise sur le marché de nouveaux outils de diagnostic permet la détection plus précoce du processus carieux de déminéralisation. Ce présent article fait le point sur le diagnostic de la carie et sur les ... [more ▼]

La mise sur le marché de nouveaux outils de diagnostic permet la détection plus précoce du processus carieux de déminéralisation. Ce présent article fait le point sur le diagnostic de la carie et sur les outils actuellement disponibles pour améliorer nos diagnostics et intercepter plus tôt le phénomène de déminéralisation. [less ▲]

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See detailExpression of matrix metalloproteinase-2 and mutant p53 is increased in hydatidiform mole as compared with normal placenta
Petignat, P.; Laurini, R.; Goffin, Frédéric ULg et al

in International Journal of Gynecological Cancer : Official Journal of the International Gynecological Cancer Society (2006), 16(4, JUL-AUG), 1679-1684

Matrix metalloproteinases (MMPs) are group of enzymes thought to play an important role in trophoblastic and tumor invasion. The aim of our study was to investigate the trophoblastic expression of MMPs ... [more ▼]

Matrix metalloproteinases (MMPs) are group of enzymes thought to play an important role in trophoblastic and tumor invasion. The aim of our study was to investigate the trophoblastic expression of MMPs and p53 in normal trophoblast and hydatidiform moles (HM). Paraffin sections of 45 specimens, including 14 complete hydatidiform moles (CM), 15 partial hydatidiform moles (PM), 8 atypical partial hydatidiform moles (aPM), and 8 controls were selected. Classification of HM was established on histologic criteria and supported by the DNA ploidy results. Tissue sections from each case were immunostained with monoclonal antibodies, cytokeratin-7, MMP-2, MMP-9, tissue inhibitors of metalloproteinases (TIMP)-1, and p53 wild type (p53wt) and mutant types (mutp53). Staining for cytokeratin-7 revealed a positive reaction in 93% of the samples. MMP-2 was mainly expressed in the syncytiotrophoblast of HM and found in 62% of aPM, 60% PM, and 93% CM. The mutp53 was mainly and focally expressed in syncytiotrophoblastic cells and was found in 63% of aPM, 80% PM, and 93% CM. Expression of MMP-2 and mutp53 was both significantly greater in HM vs control group (P < 0.05) and greater in CM vs PM and aPM (P < 0.05). No significant difference was observed for cytokeratin-7, MMP-9, TIMP-1, and p53wt between the HM subgroups and between HM and control group. MMP-2 and mutp53 are overexpressed in HM as compared with normal trophoblast and might participate in the invasive behavior of the HM. [less ▲]

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See detailLes interactions entre l’économie sociale et la responsabilité sociale des entreprises. Illustration à travers la filière du commerce équitable
Huybrechts, Benjamin ULg; Mertens de Wilmars, Sybille ULg; Xhauflair, Virginie ULg

in Gestion : Revue Internationale de Gestion (2006), 31(2), 65-74

L’étude de la responsabilité sociale des entreprises (RSE) cherche à analyser les démarches volontaires dans lesquelles des entreprises à but de lucre s'engagent afin de respecter, au-delà de leur ... [more ▼]

L’étude de la responsabilité sociale des entreprises (RSE) cherche à analyser les démarches volontaires dans lesquelles des entreprises à but de lucre s'engagent afin de respecter, au-delà de leur objectif de rentabilité économique, des exigences sociales et environnementales, et d’améliorer les relations avec toutes les parties concernées par leurs activités (Bender et Pigeyre, 2003). Ces initiatives rappellent des pratiques à l'œuvre depuis plus d'un siècle dans les organisations d'économie sociale (OES), qui essayent d'atteindre des objectifs sociétaux par le biais d'une activité économique (Defourny et Develtere, 1999). Si, à la suite de Levi (1998), on place les entreprises privées "classiques" et les OES aux extrémités d'un continuum qui s'étendrait de la "performance économique" à la "performance sociétale", on peut émettre l'hypothèse selon laquelle l'apparition de la RSE provoque un double mouvement. D'une part, les entreprises privées recherchent une certaine performance sociétale et, d'autre part, les OES visent davantage de performance économique en adoptant parfois un comportement plus typiquement commercial (Weisbrod, 1998). En conséquence, alors que les rôles respectifs des différentes catégories d’acteurs semblaient clairement définis, les frontières se font à présent plus floues et certains domaines d’activité sont indubitablement témoins d’un rapprochement entre le secteur privé "classique" et le secteur de l'économie sociale. Néanmoins, ces deux ensembles ne semblent pas mus l’un vers l’autre de manière endogène et autonome : au contraire, il y a lieu de penser que les évolutions respectives sont le fruit d’influences réciproques. Notre article a pour but de faire le point sur cette hypothèse de rapprochement, en partant des rôles traditionnels des institutions au sein du système socio-économique et en observant de quelle manière le monde des entreprises et celui de l’économie sociale se sont non seulement rapprochés, mais également influencés réciproquement. Notre réflexion est structurée en trois étapes. Tout d'abord, nous présentons brièvement le contenu que nous associons aux deux concepts principaux sur lesquels repose notre argumentation (l'économie sociale et la RSE) et nous nous basons sur les arguments de la théorie économique institutionnelle pour identifier les atouts respectifs et les rôles traditionnellement dévolus aux grandes catégories d'acteurs dans nos économies. Dans un deuxième temps, nous montrons pourquoi on peut considérer la rencontre entre les OES et les entreprises à but de profit comme un des stimuli du mouvement de RSE et combien ces interactions peuvent potentiellement transformer les comportements de ces deux types d'acteurs. Enfin, nous illustrons notre hypothèse par une analyse des interactions entre OES et entreprises en quête de responsabilité sociale dans la filière du commerce équitable et éthique. Pour ce faire, nous nous basons principalement sur une étude empirique exploratoire menée sur la scène équitable belge et portant sur ces interactions et leurs conséquences en termes de gestion de l’activité économique (Huybrechts et Mertens, 2006). [less ▲]

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See detailCytoreductive Surgery and Hyperthermic Intraperitoneal Chemotherapy for Colorectal Peritoneal Carcinomatosis : Higher Complication Rate for Oxaliplatin Compared to Mitomycin C
Rouers, A.; Laurent, S.; Detroz, Bernard ULg et al

in Acta Chirurgica Belgica (2006), 106

Peritoneal carcinomatosis (PC) from colo-rectal cancer carries a very poor prognosis with a mean and median overall survival times of 6.9 and 5.2 months. It has been proved that a locoregional therapeutic ... [more ▼]

Peritoneal carcinomatosis (PC) from colo-rectal cancer carries a very poor prognosis with a mean and median overall survival times of 6.9 and 5.2 months. It has been proved that a locoregional therapeutic approach of this disease with cytoreduction followed by hyperthermic intraperitoneal chemotherapy (HIPEC) improved survival of these patients. However, this combined treatment presents a high complication rate. Methods : 21 patients with PC of colorectal origin underwent complete cytoreduction followed by HIPEC using Mitomycin-C (13 patients) or oxaliplatin (8 patients) and the open coliseum technique. For each case the medical datas were retrospectively analysed to determine feasibility, morbidity, mortality, survival time and prognostic factors. Results : All patients presented a Sugarbaker’s Peritoneal Cancer index inferior to 15. The mean operating time was 453 minutes. After a median follow-up of 24.9 months, actuarial disease-free survival was 36.6% at 5 years. The median survival time was 34 months. The morbidity rate was 33.3% with a significant higher complication rate in the oxaliplatin group (5/8) than in the Mytomycin-C (MMC) group (2/13). One patient (4.7%) died two months after treatment with MMC (endocarditis). Conclusions : This series confirm positive impact of cytoreduction and HIPEC on PC. We obtained a moderated complications rate thanks to a high degree of selection of the patient. Oxaliplatin scheme is responsible of a higher morbidity than in MMC group. Phase III trial comparing these two drugs is needed. [less ▲]

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See detailThe immune system as a foundation for immunologic therapy and hematologic malignancies: a historical perspective.
Baron, Frédéric ULg; Storb, Rainer

in Bailliere's Best Practice & Research. Clinical Haematology = Best Practice & Research. Clinical Haematology (2006), 19(4), 637-53

In this review we aim to provide a historical overview of the immunotherapeutic approaches which have been developed for the treatment of hematological malignancies. After briefly summarizing the ... [more ▼]

In this review we aim to provide a historical overview of the immunotherapeutic approaches which have been developed for the treatment of hematological malignancies. After briefly summarizing the development of the theory of cancer immune surveillance, we describe how initial studies discovering the efficacy of the immune-mediated graft-versus-tumor effects after allogeneic hematopoietic cell transplantation led to new transplantation approaches (termed non-myeloablative transplantation) relying almost exclusively on graft-versus-tumor effects for tumor eradication. We then summarize important steps in the development of tumor vaccines and autologous adoptive immunotherapy in patients with hematological malignancies. Finally, we describe historical discoveries leading to the recent success with monoclonal antibodies as treatment for lymphomas, chronic lymphocytic leukemia, and acute myeloid leukemia. [less ▲]

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See detailVariation in fatty acid contents of milk and milk fat within and across breeds
Soyeurt, Hélène ULg; Dardenne, Pierre; Gillon, Alain ULg et al

in Journal of Dairy Science (2006), 89(12), 4858-4865

The aim of this research was to study the potential for selection of cows with a higher nutritional quality of milk fat by studying the differences in fatty acid profiles within and across the following ... [more ▼]

The aim of this research was to study the potential for selection of cows with a higher nutritional quality of milk fat by studying the differences in fatty acid profiles within and across the following breeds: Dual Purpose Belgian Blue, Holstein-Friesian, Jersey, Montbeliarde, and non-Holstein Meuse-Rhine-Yssel type Red and White. Six hundred milk samples from 275 animals were taken from 7 herds. Several types of fatty acids in milk and milk fat were quantified using midinfrared spectrometry and previously obtained calibration equations. Statistical analyses were made using a mixed linear model with a random animal effect. The variance components were estimated by using REML. Results showed breed differences for the fatty acid profile. The repeatability estimate obtained in the present study may suggest the existence of moderate additive genetic variance for the fatty acid profile within each breed. Results also indicated variation for each analyzed milk component in the whole cow population studied. Genetic improvement of the nutritional quality of milk fat based on fatty acid profiles might be possible, and further research and development are warranted. [less ▲]

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See detailParticle density determination of pellets and briquettes
Rabier, Fabienne; Temmerman, Michaël ULg; Böhm, Thorsten et al

in Biomass & Bioenergy (2006)

Several methods and procedures for the determination of particle density of pellets and briquettes were tested and evaluated. Round robin trials were organized involving five European laboratories, which ... [more ▼]

Several methods and procedures for the determination of particle density of pellets and briquettes were tested and evaluated. Round robin trials were organized involving five European laboratories, which measured the particle densities of 15 pellet and five briquette types. The test included stereometric methods, methods based on liquid displacement (hydrostatic and buoyancy) applying different procedures and one method based on solid displacement. From the results for both pellets and briquettes, it became clear that the application of a method based on either liquid or solid displacement (only tested on pellet samples) leads to an improved reproducibility compared to a stereometric method. For both, pellets and briquettes, the variability of measurements strongly depends on the fuel type itself. For briquettes, the three methods tested based on liquid displacement lead to similar results. A coating of the samples with paraffin did not improve the repeatability and the reproducibility. Determinations with pellets proved to be most reliable when the buoyancy method was applied using a wetting agent to reduce surface tensions without sample coating. This method gave the best values for repeatability and reproducibility, thus less replications are required to reach a given accuracy level. For wood pellets, the method based on solid displacement gave better values of repeatability, however, this instrument was tested at only one laboratory. [less ▲]

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See detailMethods for size classification of wood chips
Hartmann, Hans; Böhm, Thorsten; Daugbjerg Jensen, Peter et al

in Biomass & Bioenergy (2006)

Methods for size classification of wood chips were analysed in an international round robin using 13 conventional wood chip samples and two specially prepared standard samples, one from wood chips and one ... [more ▼]

Methods for size classification of wood chips were analysed in an international round robin using 13 conventional wood chip samples and two specially prepared standard samples, one from wood chips and one from hog fuel. The true size distribution of these two samples (according to length, width and height) had been determined stereometrically (reference method) using a digital calliper gauge and by weighing each of the about 7000 wood particles per sample. Five different horizontal and three rotary screening devices were tested using five different screen hole diameters (3.15, 8, 16, 45, 63 mm, round holes). These systems are compared to a commercially available continuously measuring image analysis equipment. The results show that among the devices of a measuring principle—horizontal and rotary screening—the results are quite comparable, while there is a severe incompatibility when distributions are determined by different measuring principles. Highest conformity with the reference values is given for measurements with an image analysis system, whereas for all machines with horizontal screens the median value of the size distribution only reached between one-third to half of the reference median value for the particle length distribution. These deviations can be attributed to a higher particle misplacement, which is particularly found in the larger fractions. Such differences decrease when the particle’s shape is more roundish (i.e. sphericity closer to one). The median values of length distributions from screenings with a rotary classifier are between the measurements from an image analysis and horizontal screening devices. [less ▲]

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See detailInternal particle size distribution of biofuel pellets
Daugbjerg Jensen, Peter; Temmerman, Michaël ULg; Westborg, Suzanne

in Fuel (2006)

Several methods for disintegration of biofuel pellets were tested and compared for their ability to break up the pellets into the original particles of the raw material. Analyses performed on softwood ... [more ▼]

Several methods for disintegration of biofuel pellets were tested and compared for their ability to break up the pellets into the original particles of the raw material. Analyses performed on softwood pellets and straw pellets concluded that wet disintegration in water at ambient temperature is insufficient for a determination of the internal particle size distribution of wood- and straw pellets. When the wet disintegration was performed with water heated to the boiling point and coupled with mechanical disintegration in terms of stirring a more complete disintegration of the pellets was obtained. Based on the results obtained in the initial study a round robin was set up including six European laboratories where the selected method was tested. In the round robin test the method combining heated water and stirring of the slurry was tested on solid biofuel pellets produced of comminute straw, deciduous wood and coniferous wood respectively. With the method a satisfactory disintegration was obtained of all three types of pellets. Further wet disintegration of coniferous pellets was compared to a dry disintegration using a hammer mill. The dry disintegration of the coniferous pellets resulted in smaller particle sizes compared to the wet disintegration using heated water and stirring of the slurry indicating a further disintegration of the original particles in the hammer mill process. Overall the wet disintegration combined with mechanical impact was found to be the most suitable method for disintegration of solid biofuel pellets. Combined with sieving analysis the method gives realistic image of the internal particle size distribution of solid biofuel pellets. [less ▲]

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See detailComparative study of durability test methods for pellets and briquettes
Temmerman, Michaël ULg; Rabier, Fabienne; Daugbjerg Jensen, Peter et al

in Biomass & Bioenergy (2006)

Different methods for the determination of the mechanical durability (DU) of pellets and briquettes were compared by international round robin tests including different laboratories. The DUs of five ... [more ▼]

Different methods for the determination of the mechanical durability (DU) of pellets and briquettes were compared by international round robin tests including different laboratories. The DUs of five briquette and 26 pellet types were determined. For briquettes, different rotation numbers of a prototype tumbler and a calculated DU index are compared. For pellets testing, the study compares two standard methods, a tumbling device according to ASAE S 269.4, the Lignotester according to O¨ NORM M 7135 and a second tumbling method with a prototype tumbler. For the tested methods, the repeatability, the reproducibility and the required minimum number of replications to achieve given accuracy levels were calculated. Additionally, this study evaluates the relation between DU and particle density. The results show for both pellets and briquettes, that the measured DU values and their variability are influenced by the applied method. Moreover, the variability of the results depend on the biofuel itself. For briquettes of DU above 90%, five replications lead to an accuracy of 2%, while 39 replications are needed to achieve an accuracy of 10%, when briquettes of DU below 90% are tested. For pellets, the tumbling device described by the ASAE standard allows to reach acceptable accuracy levels (1%) with a limited number of replications. Finally, for the tested pellets and briquettes no relation between DU and particle density was found. [less ▲]

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