Publications and communications of Paulette Charlier

Léonard, R., Sauvage, E., Lupo, V., Perrin, A., Sirjacobs, D., Charlier, P., Kerff, F., & Baurain, D. (18 February 2022). Was the last bacterial common ancestor a monoderm after all? Genes, 13. doi:10.3390/genes13020376

vettore, N., Moray, J., Brans, A., Herman, R., Charlier, P., kumita, J., Kerff, F., Dobson, C., & Dumoulin, M. (2021). Characterisation of the structural, dynamic and aggregation properties of the W64R amyloidogenic variant of human lysozyme. Biophysical Chemistry. doi:10.1016/j.bpc.2021.106563

Azarkan, M., Feller, G., Vandenameele, J., Herman, R., El Mahyaoui, R., Sauvage, E., Vanden Broeck, A., Matagne, A., Charlier, P., & Kerff, F. (2018). Biochemical and structural characterization of a mannose binding jacalin-related lectin with two-sugar binding sites from pineapple (Ananas comosus) stem. Scientific Reports. doi:10.1038/s41598-018-29439-x

Rodríguez, M. M., Herman, R., Ghiglione, B., Kerff, F., D'Amico González, G., Bouillenne, F., Galleni, M., Handelsman, J., Charlier, P., Gutkind, G., Sauvage, E., & Power, P. (July 2017). Crystal Structure and Kinetic Analysis of the Class B3 Di-Zinc Metallo-β-Lactamase LRA-12 from an Alaskan Soil Metagenome. PLoS ONE, 12 (7), 0182043. doi:10.1371/journal.pone.0182043

Ruggiero, M., Kerff, F., Herman, R., Sapunaric, F., Galleni, M., Gutkind, G., Charlier, P., Sauvage, E., & Power, P. (2014). Crystal Structure of the Extended-Spectrum β -Lactamase PER-2 and Insights into the Role of Specific Residues in the Interaction with β -Lactams and β -Lactamase Inhibitors. Antimicrobial Agents and Chemotherapy, 58 (10), 5994-6002. doi:10.1128/AAC.00089-14

Sauvage, E., Derouaux, A., Fraipont, C., Joris, M., Herman, R., Rocaboy, M., Schloesser, M., Dumas, J., Kerff, F., Nguyen-Distèche, M., & Charlier, P. (2014). Crystal structure of penicillin-binding protein 3 (PBP3) from Escherichia coli. PLoS ONE. doi:10.1371/journal.pone.0098042

Dzhekieva, L., Adediran, S. A., Herman, R., Kerff, F., Duez, C., Charlier, P., Sauvage, E., & Pratt, R. F. (2013). Inhibition of dd-Peptidases by a Specific Trifluoroketone: Crystal Structure of a Complex with the Actinomadura R39 dd-Peptidase. Biochemistry. doi:10.1021/bi400048s

Rocaboy, M., Herman, R., Sauvage, E., Remaut, H., Moonens, K., Terrak, M., Charlier, P., & Kerff, F. (2013). The crystal structure of the cell division amidase AmiC reveals the fold of the AMIN domain, a new peptidoglycan binding domain. Molecular Microbiology. doi:10.1111/mmi.12361

Zervosen, A., Bouillez, A., Herman, A., Amoroso, A. M., Joris, B., Sauvage, E., Charlier, P., & Luxen, A. (2012). Synthesis and evaluation of boronic acids as inhibitors of Penicillin Binding Proteins of classes A, B and C. Bioorganic and Medicinal Chemistry, 20 (12), 3915-24. doi:10.1016/j.bmc.2012.04.018

Zervosen, A., Herman, R., Kerff, F., Herman, A., Bouillez, A., Prati, F., Pratt, R. F., Frère, J.-M., Joris, B., Luxen, A., Charlier, P., & Sauvage, E. (2011). Unexpected tricovalent binding mode of boronic acids within the active site of a penicillin binding protein. Journal of the American Chemical Society. doi:10.1021/ja200696y

Woon, E. C. Y., Zervosen, A., Sauvage, E., Simmons, K. J., Zivec, M., Inglis, S. R., Fishwick, C. W. G., Gobec, S., Charlier, P., Luxen, A., & Schofield, C. J. (2011). Structure Guided Development of Potent Reversibly Binding Penicillin Binding Protein Inhibitors. ACS Medicinal Chemistry Letters, 2 (3), 219-223. doi:10.1021/ml100260x

Brown, T. J., Charlier, P., Herman, R., Schofield, C. J., & Sauvage, E. (2010). Structural basis for the interaction of lactivicins with serine beta-lactamases. Journal of Medicinal Chemistry, 53 (15), 5890-4. doi:10.1021/jm100437u

Sauvage, E., Zervosen, A., Dive, G., Herman, R., Amoroso, A. M., Joris, B., Fonzé, E., Pratt, R., Luxen, A., Charlier, P., & Kerff, F. (2009). Structural basis of the inhibition of class A beta-lactamases and penicillin-binding proteins by 6-beta-iodopenicillanate. Journal of the American Chemical Society, 131 (42), 15262-15269. doi:10.1021/ja9051526

Beck, J., Sauvage, E., Charlier, P., & Marchand-Brynaert, J. (2008). 2-Aminopropane-1,2,3-tricarboxylic acid: Synthesis and co-crystallization with the class A beta-lactamase BS3 of Bacillus licheniformis. Bioorganic and Medicinal Chemistry Letters, 18 (13), 3764-8. doi:10.1016/j.bmcl.2008.05.045

Michaux, C., Massant, J., Kerff, F., Frère, J.-M., Docquier, J.-D., Vandenberghe, I., Samyn, B., Pierrard, A., Feller, G., Charlier, P., Van Beeumen, J., & Wouters, J. (2008). Crystal structure of a cold-adapted class C beta-lactamase. FEBS Journal, 275 (8), 1687-97. doi:10.1111/j.1742-4658.2008.06324.x

Sauvage, E., Powell, A. J., Heilemann, J., Josephine, H. R., Charlier, P., Davies, C., & Pratt, R. F. (2008). Crystal structures of complexes of bacterial DD-peptidases with peptidoglycan-mimetic ligands: the substrate specificity puzzle. Journal of Molecular Biology, 381 (2), 383-93. doi:10.1016/j.jmb.2008.06.012

Sauvage, E., Duez, C., Herman, R., Kerff, F., Petrella, S., Anderson, J. W., Adediran, S. A., Pratt, R. F., Frère, J.-M., & Charlier, P. (10 August 2007). Crystal structure of the Bacillus subtilis penicillin-binding protein 4a, and its complex with a peptidoglycan mimetic peptide. Journal of Molecular Biology, 371 (2), 528-539. doi:10.1016/j.jmb.2007.05.071

Josephine, H. R., Charlier, P., Davies, C., Nicholas, R. A., & Pratt, R. F. (2006). Reactivity of penicillin-binding proteins with peptidoglycan-mimetic beta-lactams: what's wrong with these enzymes? Biochemistry, 45 (51), 15873-83. doi:10.1021/bi061804f

Sauvage, E., Fonze, E., Quinting, B., Galleni, M., Frère, J.-M., & Charlier, P. (2006). Crystal structure of the Mycobacterium fortuitum class A beta-lactamase: structural basis for broad substrate specificity. Antimicrobial Agents and Chemotherapy, 50 (7), 2516-21. doi:10.1128/AAC.01226-05

Michaux, C., Charlier, P., Frère, J.-M., & Wouters, J. (2005). Crystal structure of BRL 42715, C6-(N1-methyl-1,2,3-triazolylmethylene)penem, in complex with Enterobacter cloacae 908R beta-lactamase: evidence for a stereoselective mechanism from docking studies. Journal of the American Chemical Society, 127 (10), 3262-3. doi:10.1021/ja0426241

Salvagnini, C., Michaux, C., Remiche, J., Wouters, J., Charlier, P., & Marchand-Brynaert, J. (2005). Design, synthesis and evaluation of graftable thrombin inhibitors for the preparation of blood-compatible polymer materials. Organic and Biomolecular Chemistry, 3 (23), 4209-20. doi:10.1039/b510239a

Sauvage, E., Herman, R., Petrella, S., Duez, C., Bouillenne, F., Frère, J.-M., & Charlier, P. (2005). Crystal structure of the Actinomadura R39 DD-peptidase reveals new domains in penicillin-binding proteins. Journal of Biological Chemistry, 280 (35), 31249-56. doi:10.1074/jbc.M503271200

Anderson, J. W., Adediran, S. A., Charlier, P., Nguyen-Distèche, M., Frère, J.-M., Nicholas, R. A., & Pratt, R. F. (01 August 2003). On the substrate specificity of bacterial DD-peptidases: evidence from two series of peptidoglycan-mimetic peptides. Biochemical Journal, 373 (Part 3), 949-955. doi:10.1042/BJ20030217

Wouters, J., Fonze, E., Vermeire, M., Frère, J.-M., & Charlier, P. (August 2003). Crystal structure of Enterobacter cloacae 908R class C beta-lactamase bound to iodo-acetamido-phenyl boronic acid, a transition-state analogue. Cellular and Molecular Life Sciences, 60 (8), 1764-1773. doi:10.1007/s00018-003-3189-2

Fonzé, E., Vanhove, M., Dive, G., Sauvage, E., Frère, J.-M., & Charlier, P. (February 2002). Crystal structures of the Bacillus licheniformis BS3 class A beta-lactamase and of the acyl-enzyme adduct formed with cefoxitin. Biochemistry, 41 (6), 1877-1885. doi:10.1021/bi015789k

Sauvage, E., Kerff, F., Fonze, E., Herman, R., Schoot, B., Marquette, J. P., Taburet, Y., Prevost, D., Dumas, J., Stefanic, P., Coyette, J., Charlier, P., & Leonard, G. (2002). The 2.4-A crystal structure of the penicillin-resistant penicillin-binding protein PBP5fm from Enterococcus faecium in complex with benzylpenicillin. Cellular and Molecular Life Sciences, 59 (7), 1223-32. doi:10.1007/s00018-002-8500-0

Wouters, J., Charlier, P., Monnaie, D., Frère, J.-M., & Fonze, E. (2001). Expression, purification, crystallization and preliminary X-ray analysis of the native class C beta-lactamase from Enterobacter cloacae 908R and two mutants. Acta Crystallographica. Section D, Biological Crystallography, 57 (Pt 1), 162-4. doi:10.1107/S0907444900016413

Wouters, J., Georis, J., Engher, D., Vandenhaute, J., Dusart, J., Frère, J.-M., Depiereux, E., & Charlier, P. (2001). Crystallographic analysis of family 11 endo-beta-1,4-xylanase Xyl1 from Streptomyces sp. S38. Acta Crystallographica. Section D, Biological Crystallography, 57 (Pt 12), 1813-9. doi:10.1107/S0907444901015153

Kelly, J. A., Kuzin, A. P., Charlier, P., & Fonze, E. (1998). X-ray studies of enzymes that interact with penicillins. Cellular and Molecular Life Sciences, 54 (4), 353-8. doi:10.1007/s000180050163

Ledent, P., Duez, C., Vanhove, M., Lejeune, A., Fonze, E., Charlier, P., Rhazi-Filali, F., Thamm, I., Guillaume, G., Samyn, B., Devreese, B., Van Beeumen, J., Lamotte-Brasseur, J., & Frère, J.-M. (1997). Unexpected Influence of a C-Terminal-Fused His-Tag on the Processing of an Enzyme and on the Kinetic and Folding Parameters. FEBS Letters, 413 (2), 194-196. doi:10.1016/S0014-5793(97)00908-3

Ghuysen, J.-M., Charlier, P., Coyette, J., Duez, C., Fonze, E., Fraipont, C., Joris, B., Nguyen-Distèche, M., & Goffin, C. (1996). Penicillin and Beyond: Evolution, Protein Fold, Multimodular Polypeptides, and Multiprotein Complexes. Microbial Drug Resistance: Mechanism, Epidemiology, and Disease, 2 (2, Summer), 163-175. doi:10.1089/mdr.1996.2.163

Fonze, E., Charlier, P., To'th, Y., Vermeire, M., Raquet, X., Dubus, A., & Frère, J.-M. (1995). TEM1 beta-lactamase structure solved by molecular replacement and refined structure of the S235A mutant. Acta Crystallographica. Section D, Biological Crystallography, 51 (Pt 5), 682-94. doi:10.1107/S0907444994014496

Englebert, S., Charlier, P., Fonze, E., To'th, Y., Vermeire, M., Van Beeumen, J., Grandchamps, J., Hoffmann, K., Leyh-Bouille, M., Nguyen-Distèche, M., & Ghuysen, J.-M. (1994). Crystallization and X-ray diffraction study of the Streptomyces K15 penicillin-binding DD-transpeptidase. Journal of Molecular Biology, 241 (2), 295-7. doi:10.1006/jmbi.1994.1504

Charlier, P., Buisson, G., Dideberg, O., Wierenga, J., Keck, W., Laible, G., & Hakenbeck, R. (1993). Crystallization of a genetically engineered water-soluble primary penicillin target enzyme. The high molecular mass PBP2x of Streptococcus pneumoniae. Journal of Molecular Biology, 232 (3), 1007-9. doi:10.1006/jmbi.1993.1452

Charlier, P., Jérôme, R., & Teyssié, P. (1993). Solution behaviour of α,ε{lunate}-(dimethylamino) polymers quaternized with alkyl iodides. Polymer, 34 (2), 369-375. doi:10.1016/0032-3861(93)90091-N

Charlier, P., Jérôme, R., Teyssié, P., & Proud'Homme, R. E. (1993). Thermal stability of modified telechelic polystyrenes. Journal of Polymer Science. Part A, Polymer Chemistry, 31 (1), 129-134. doi:10.1002/pola.1993.080310115

Charlier, P., Jérôme, R., Teyssié, P., & Utracki, L. A. (1992). Viscoelastic Properties of Telechelic Ionomers. 2. Quaternized α,ω-Bis(dimethylamino)polyisoprene. Macromolecules, 25 (2), 617-624. doi:10.1021/ma00028a021

Charlier, P., Jérôme, R., Teyssié, P., & Utracki, L. A. (1992). Viscoelastic Properties of Immiscible Telechelic Polymer Blends: Effect of Acid-Base Interactions. Macromolecules, 25 (10), 2651-2656. doi:10.1021/ma00036a015

Lamotte-Brasseur, J., Dive, G., Dideberg, O., Charlier, P., Frère, J.-M., & Ghuysen, J.-M. (01 October 1991). Mechanism of acyl transfer by the class A serine β-lactamase of Streptomyces albus G. Biochemical Journal, 279 (Pt 1), 213-221. doi:10.1042/bj2790213

Marcq, S., Diaz-Ruano, A., Charlier, P., Dideberg, O., Tricot, C., Stalon, V., & Piérard, A. (1991). Molecular size and symmetry of Pseudomonas aeruginosa catabolic ornithine carbamoyltransferase. An X-ray crystallography analysis. Journal of Molecular Biology, 220 (1), 9-12. doi:10.1016/0022-2836(91)90375-G

Moews, P. C., Knox, J. R., Dideberg, O., Charlier, P., & Frère, J.-M. (1990). Beta-lactamase of Bacillus licheniformis 749/C at 2 A resolution. Proteins, 7 (2), 156-71. doi:10.1002/prot.340070205

Joris, B., Ghuysen, J.-M., Dive, G., Renard, A., Dideberg, O., Charlier, P., Frère, J.-M., Kelly, J. A., Boyington, J., Moews, P. C., & Knox, J. (01 March 1988). The Active-Site-Serine Penicillin-Recognizing Enzymes as Members of the Streptomyces R61 Dd-Peptidase Family. Biochemical Journal, 250 (2), 313-324. doi:10.1042/bj2500313

Ferreira, L. C., Schwarz, U., Keck, W., Charlier, P., Dideberg, O., & Ghuysen, J.-M. (15 January 1988). Properties and crystallization of a genetically engineered, water-soluble derivative of penicillin-binding protein 5 of Escherichia coli K12. European Journal of Biochemistry, 171 (1-2), 11-16. doi:10.1111/j.1432-1033.1988.tb13751.x

Frère, J.-M., Joris, B., Dideberg, O., Charlier, P., & Ghuysen, J.-M. (1988). Penicillin-recognizing enzymes. Biochemical Society Transactions, 16 (6), 934-938. doi:10.1042/bst0160934

Dideberg, O., Charlier, P., Wery, J.-P., Dehottay, P., Dusart, J., Erpicum, T., Frère, J.-M., & Ghuysen, J.-M. (1987). The crystal structure of the β-lactamase of Streptomyces albus G at 0.3 nm resolution. Biochemical Journal, 245 (3), 911-913. doi:10.1042/bj2450911

Kelly, J. A., Dideberg, O., Charlier, P., Wery, J.-P., Libert, M., Moews, P., Knox, J., Duez, C., Fraipont, C., Joris, B., Dusart, J., Frère, J.-M., & Ghuysen, J.-M. (21 March 1986). On the origin of bacterial resistance to penicillin: comparison of a beta-lactamase and a penicillin target. Science, 231, 1429-1431. doi:10.1126/science.3082007

Charlier, P., Dideberg, O., Jamoulle, J.-C., Frère, J.-M., Ghuysen, J.-M., Dive, G., & Lamotte, J. (1984). Active-site-directed inactivators of the Zn2+-containing D-alanyl-D-alanine-cleaving carboxypeptidase of Streptomyces albus G. Biochemical Journal, 219 (3), 763-772. doi:10.1042/bj2190763

Ghuysen, J.-M., Frère, J.-M., Leyh-Bouille, M., Nguyen-Distèche, M., Coyette, J., Dusart, J., Joris, B., Duez, C., Dideberg, O., Charlier, P., Dive, G., & Lamotte-Brasseur, J. (1984). Bacterial cell wall, DD-peptidase and β-lactam antibiotics. Scandinavian Journal of Infectious Diseases, 42, 17-37.

Labischinski, H., Giesbrecht, P., Fischer, E., Barnickel, G., Bradaczek, H., Frère, J.-M., Houssier, C., Charlier, P., Dideberg, O., & Ghuysen, J.-M. (1984). Study of the Zn-containing DD-carboxypeptidase of Streptomyces albus G by small-angle X-ray scattering in solution. European Journal of Biochemistry, 138 (1), 83-87. doi:10.1111/j.1432-1033.1984.tb07884.x

Dideberg, O., Charlier, P., Dive, G., Joris, B., Frère, J.-M., & Ghuysen, J.-M. (1982). Structure of a Zn2+-containing D-alanyl-D-alanine-cleaving carboxypeptidase at 2.5 A resolution. Nature, 299 (5882), 469-470. doi:10.1038/299469a0

Dideberg, O., Charlier, P., Dupont, L., Vermeire, M., Frère, J.-M., & Ghuysen, J.-M. (1980). The 4.5 Å resolution structure analysis of the exocellular DD-carboxypeptidase of Streptomyces albus G. FEBS Letters, 117 (1), 212-214. doi:10.1016/0014-5793(80)80947-1

Charlier, P., Coyette, J., Dideberg, O., Duez, C., Dusart, J., Frère, J.-M., Ghuysen, J.-M., Joris, B., Leyh-Bouille, M., & Nguyen-Distèche, M. (1980). On the Active Centers of Serine and Zn II DD-carboxyppetidases. In G. I. Gregory (Ed.), Recent advances in the Chemistry of beta-lactam antibiotics (pp. 184-202). London, United Kingdom: The Royal Society of Chemistry.