Unpublished conference/Abstract (Scientific congresses and symposiums)
HPV triggers NK cell cytotoxic activity and cytokine secretion
Jacobs, Nathalie; Renoux, Virginie; Bisig, Bettina et al.
201127th International Papillomavirus Conference
 

Files


Full Text
Berlin poster 11.pdf
Author preprint (3.41 MB)
Download

All documents in ORBi are protected by a user license.

Send to



Details



Keywords :
NK cells; immunology; HPV; Infection; CD16; Cytotoxicity
Abstract :
[en] Background The immune system controls, at least partially, human papillomavirus (HPV) infection and subsequent tumor development as demonstrated by a higher tumor prevalence in immunodeficient patients. Around 90% of HPV-infected women will clear the virus within two years. However, it remains unclear which immune cells are implicated in this process and no study has been performed evaluating the direct interaction between HPV and Natural Killer (NK) cells although these cells play a key role in host resistance to virus and tumor. Methods/Results By immunochemistry, we demonstrated an NK cell infiltration in HPV+ squamous pre-neoplasic lesions. Since HPV cannot grow in vitro, virus-like particles (VLP) were used as a model for studying the NK cell response against the virus. Interestingly, NK cells displayed a higher cytotoxic activity (CD107 and chromium release assays) and cytokine production (TNF-α and IFN-γ) in the presence of HPV-VLP. Uptake of HPV-VLP by dendritic cells (DC) has been shown to induce their activation, therefore, we investigated by flow cytometry and microscopy whether the stimulation of NK cell activity is linked to VLP internalization. We observed a faster entry into these cells compared to DC. Furthermore, virus uptake by NK cells is mediated by macropinocytosis, whereas this entry is dependent on clathrin or caveolin endocytosis pathways in DC. Using NK cell lines expressing or not CD16 and blocking antibody, we demonstrated that CD16 is necessary for HPV-VLP internalization, but also for degranulation and cytokine production. Conclusion Thus, we show for the first time that NK cells interact with HPV and could participate in the immune response against HPV-induced tumors.
Disciplines :
Oncology
Microbiology
Immunology & infectious disease
Author, co-author :
Jacobs, Nathalie  ;  Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Anatomie et cytologie pathologiques
Renoux, Virginie ;  Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Anatomie et cytologie pathologiques
Bisig, Bettina ;  Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Anatomie et cytologie pathologiques
Langers, Inge ;  Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Anatomie et cytologie pathologiques
Dortu, Estelle ;  Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Anatomie et cytologie pathologiques
Clémenceau, Béatrice;  Université de Nantes, France
Thiry, Marc  ;  Université de Liège - ULiège > Département des sciences de la vie > Biologie cellulaire
Deroanne, Christophe ;  Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Laboratoire des tissus conjonctifs
Boniver, Jacques ;  Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Département des sciences biomédicales et précliniques
Delvenne, Philippe ;  Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Anatomie et cytologie pathologiques
Language :
English
Title :
HPV triggers NK cell cytotoxic activity and cytokine secretion
Publication date :
2011
Event name :
27th International Papillomavirus Conference
Event place :
Berlin, Germany
Event date :
17-22/9/2011
Audience :
International
Funders :
F.R.S.-FNRS - Fonds de la Recherche Scientifique [BE]
Télévie [BE]
CAC - Centre anticancéreux près l'Université de Liège asbl [BE]
Available on ORBi :
since 10 October 2011

Statistics


Number of views
199 (6 by ULiège)
Number of downloads
121 (2 by ULiège)

Bibliography


Similar publications



Contact ORBi