Reference : HPV triggers NK cell cytotoxic activity and cytokine secretion
Scientific congresses and symposiums : Unpublished conference
Human health sciences : Oncology
Life sciences : Microbiology
Human health sciences : Immunology & infectious disease
http://hdl.handle.net/2268/99925
HPV triggers NK cell cytotoxic activity and cytokine secretion
English
Jacobs, Nathalie mailto [Université de Liège - ULg > Département des sciences biomédicales et précliniques > Anatomie et cytologie pathologiques >]
Renoux, Virginie mailto [Université de Liège - ULg > Département des sciences biomédicales et précliniques > Anatomie et cytologie pathologiques >]
Bisig, Bettina [Université de Liège - ULg > Département des sciences biomédicales et précliniques > Anatomie et cytologie pathologiques >]
Langers, Inge [Université de Liège - ULg > Département des sciences biomédicales et précliniques > Anatomie et cytologie pathologiques >]
Dortu, Estelle [Université de Liège - ULg > Département des sciences biomédicales et précliniques > Anatomie et cytologie pathologiques >]
Clémenceau, Béatrice [Université de Nantes, France > > > >]
Thiry, Marc mailto [Université de Liège - ULg > Département des sciences de la vie > Biologie cellulaire >]
Deroanne, Christophe [Université de Liège - ULg > Département des sciences biomédicales et précliniques > Laboratoire des tissus conjonctifs >]
Boniver, Jacques [Université de Liège - ULg > Département des sciences biomédicales et précliniques > Département des sciences biomédicales et précliniques >]
Delvenne, Philippe [Université de Liège - ULg > Département des sciences biomédicales et précliniques > Anatomie et cytologie pathologiques >]
2011
Yes
International
27th International Papillomavirus Conference
17-22/9/2011
Berlin
Germany
[en] NK cells ; immunology ; HPV ; Infection ; CD16 ; Cytotoxicity
[en] Background
The immune system controls, at least partially, human papillomavirus (HPV) infection and subsequent tumor development as demonstrated by a higher tumor prevalence in immunodeficient patients. Around 90% of HPV-infected women will clear the virus within two years. However, it remains unclear which immune cells are implicated in this process and no study has been performed evaluating the direct interaction between HPV and Natural Killer (NK) cells although these cells play a key role in host resistance to virus and tumor.
Methods/Results
By immunochemistry, we demonstrated an NK cell infiltration in HPV+ squamous pre-neoplasic lesions. Since HPV cannot grow in vitro, virus-like particles (VLP) were used as a model for studying the NK cell response against the virus. Interestingly, NK cells displayed a higher cytotoxic activity (CD107 and chromium release assays) and cytokine production (TNF-α and IFN-γ) in the presence of HPV-VLP. Uptake of HPV-VLP by dendritic cells (DC) has been shown to induce their activation, therefore, we investigated by flow cytometry and microscopy whether the stimulation of NK cell activity is linked to VLP internalization. We observed a faster entry into these cells compared to DC. Furthermore, virus uptake by NK cells is mediated by macropinocytosis, whereas this entry is dependent on clathrin or caveolin endocytosis pathways in DC. Using NK cell lines expressing or not CD16 and blocking antibody, we demonstrated that CD16 is necessary for HPV-VLP internalization, but also for degranulation and cytokine production.
Conclusion
Thus, we show for the first time that NK cells interact with HPV and could participate in the immune response against HPV-induced tumors.
Fonds de la Recherche Scientifique (Communauté française de Belgique) - F.R.S.-FNRS ; televie-FNRS ; CAC
Researchers ; Professionals
http://hdl.handle.net/2268/99925

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