Article (Scientific journals)
The CMC-like behaviour of bile salts as probed by photoexcited Rose Bengal
Seret, Alain; Bahri, Mohamed Ali
2009In Colloids and Surfaces A: Physicochemical and Engineering Aspects, 339, p. 153-158
Peer Reviewed verified by ORBi
 

Files


Full Text
COLSUA09_PostPrintAuthor.pdf
Author postprint (1.46 MB)
Download

The original publication is available at www.sciencedirect.com


All documents in ORBi are protected by a user license.

Send to



Details



Keywords :
Bile salts; Aggregation process; Critical Micelle Concentration; Micellar Dissociation Concentration; Dihydroxy bile salt; Trihydroxy bile salt; CHAPS; Monomers; Rose Bengal
Abstract :
[en] The onset of the aggregation process in aqueous bile salt (BS) solutions remains a debated question. This study aimed at investigating this point through the detection of BS monomers and small aggregates using the dye Rose Bengal. Results showed that a few small BS aggregates led to a red shift of the absorption spectrum, while the BS monomer reacted with the photo-induced semi-oxidized dye and decreased its decay rate constant. The dye was dissolved in aqueous solution (10 mmol dm-3 NaOH) of sodium cholate (NaC), deoxycholate (NaDC), chenodoxycholate (NaCDC), dehydrocholate (NaDHC), glycocholate (NaGC) glycodeoxycholate (NaGDC), taurocholate (NaTC), taurodeoxycholate (NaTDC) and the zwitterionic analogue of sodium cholate called CHAPS. BS aggregates were detected at concentrations well below the concentration above which any added bile salt molecules participate in the aggregation process for all trihydroxy bile salts and for CHAPS but not for the dihydroxy bile salts. No aggregation of NaDHC could be observed in the studied concentration range (≤ 10 mmol dm-3). The presence of 150 and 600 mmol dm-3 sodium ions lowered the concentration below which BS are mainly monomers for dihydroxy but not for trihydroxy bile salts. As already proposed by others, the use of the concept of Micellar Dissociation Concentration (MDC) is suggested here instead of the classical Critical Micelle Concentration (CMC).
Disciplines :
Biochemistry, biophysics & molecular biology
Chemistry
Physics
Author, co-author :
Seret, Alain ;  Université de Liège - ULiège > Département de physique > Imagerie médicale expérimentale
Bahri, Mohamed Ali  ;  Université de Liège - ULiège > Département de physique > Imagerie médicale expérimentale
Language :
English
Title :
The CMC-like behaviour of bile salts as probed by photoexcited Rose Bengal
Publication date :
28 March 2009
Journal title :
Colloids and Surfaces A: Physicochemical and Engineering Aspects
ISSN :
0927-7757
eISSN :
1873-4359
Publisher :
Elsevier Science
Volume :
339
Pages :
153-158
Peer reviewed :
Peer Reviewed verified by ORBi
Commentary :
The authors thank Elsevier for allowing deposit of their final version on the ULg institutional repository
Available on ORBi :
since 30 March 2009

Statistics


Number of views
98 (14 by ULiège)
Number of downloads
766 (4 by ULiège)

Scopus citations®
 
11
Scopus citations®
without self-citations
11
OpenCitations
 
10

Bibliography


Similar publications



Contact ORBi