Basement Membrane Components (Matrigel) Promote the Tumorigenicity of Human Breast Adenocarcinoma Mcf7 Cells and Provide an in Vivo Model to Assess the Responsiveness of Cells to Estrogen
Noël, Agnès; Simon, N.; Raus, J.et al.
1992 • In Biochemical Pharmacology, 43 (6), p. 1263-7
[en] The ability to transplant human tumors into athymic nude mice allows studies of tumor cells in vivo. However, after s.c. injection the incidence of tumor and metastases in nude mice is frequently low. We have studied the tumorigenicity in nude mice of estradiol (E2)-sensitive breast adenocarcinoma MCF7 cells. Matrigel, an extract of basement membrane proteins, induces rapid tumor development after s.c. injection of MCF7 cells. In the absence of this matrice, MCF7 cells failed to induce tumor growth. In this in vivo model, MCF7 cells were analysed for their E2 sensitivity. Two weeks after inoculation in the presence of matrigel, cells formed growing tumors in intact mice supplemented with E2. In ovariectomized or untreated mice, tumor appearance was delayed and the growth level was very low. Thus, MCF7 cells formed tumors in the absence of E2 but retained in vivo their responsiveness to estrogen. Growing human tumors in nude mice provides a rapid and useful model for testing the sensitivity of cells to hormone.
Noël, Agnès ; Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Biologie cellulaire et moléculaire appliquée à l'homme
Simon, N.
Raus, J.
Foidart, Jean-Michel ; Université de Liège - ULiège > Département des sciences cliniques > Gynécologie - Obstétrique
Language :
English
Title :
Basement Membrane Components (Matrigel) Promote the Tumorigenicity of Human Breast Adenocarcinoma Mcf7 Cells and Provide an in Vivo Model to Assess the Responsiveness of Cells to Estrogen
Giovanella, Fogh (1985) The nude mouse in cancer research. Adv Cancer Res 44:69-120.
Price, Zhang (1990) Studies of human breast cancer metastasis using nude mice. Cancer Metast Rev 8:285-297.
Miller, Heppner (1990) Cellular interactions in metastasis. Cancer Metast Rev 9:21-34.
Miller, McInerney (1988) Epithelial component of host-tumor interactions in the orthoptic site preference of a mouse mammary tumor. Cancer Res 48:3698-3701.
Turpeenniemi-Hujanen, Thorgeirsson, Rao, Liotta (1986) Laminin increases the release of type IV collagenase from malignant cells. J Cell Biochem 261:1883-1889.
McCarthy, Furcht (1984) Laminin and fibronectin promote the haptotactic migration of B 16 mouse melanoma cells in vitro. J Cell Biol 98:1474-1480.
Humphries, Yamada, Olsen (1988) Investigation of the biological effects of anti-cell adhesive synthetic peptides that inhibit experimental metastasis of B16F10 murine melanoma cells. J Clin Invest 81:782-788.
Iwamoto, Graf, Sasaki, Kleinman, Greatorex, Martin, Robey, Yamada (1988) Synthetic penta-peptide from the B1 chain of laminin promotes B16F10 melanoma cell migration. J Cell Physiol 134:287-291.
Terranova, Williams, Liotta (1984) Modulation of the metastatic activity of melanoma cells by laminin and fibronectin. Science 226:982-984.
Noël, Callé, Emonard, Nusgens, Foidart, Lapière (1988) Antagonistic effects of laminin and fibronectin in cell-to-cell and cell-to-matrix interactions in MCF-7 cultures. In Vitro Cellular & Developmental Biology 24:373-379.
Fridman, Giaccone, Kanemoto, Martin, Gazdar, Mulshine (1990) Reconstituted basement membrane (matrigel) and laminin can enhance the tumorigenicity and the drug resistance of small cell lung cancer cell lines. Proc Natl Acad Sci USA , 2nd English Edn.; 87:6698-6702.
Soule, McGrath (1980) Estrogen responsive proliferation of clonal human breast carcinoma cells in athymic mice. Cancer Res 10:177-189.
Shafie, Grantham (1981) Role of hormone in the growth and regression of human breast cancer cells (MCF7) transplanted into athymic nude mice. J Natl Cancer Inst 67:51-56.
Noël, Callé, Emonard, Nusgens, Simar, Foidart, Lapière, Foidart (1991) Invasion of reconstituted basement membrane matrix is not correlated to the malignant metastatic cell phenotype. Cancer Res 51:405-414.
Kleinman, McGarvey, Hassell, Star, Cannon, Laurie, Martin (1986) Basement membrane complexes with biological activity. Biochemistry 25:218-312.
Robinson, Jordan (1989) Antiestrogenic action of toremifene on hormone-dependent, -independent, and heterogenous breast tumor growth in the athymic mouse. Cancer Res 49:1758-1762.
Seibert, Shafie, Triche, Whang-Peng, O'Brien, Toney, Huff, Lippman (1983) Clonal variation of MCF-7 breast cancer cells in vitro and in athymic nude mice. Cancer Res 43:2223-2239.
Attia, Weiss (1966) Immunology of spontaneous mammary carcinomas in mice. V: Acquired tumor resistance and enhancement in strain a mice infected with mammary tumor virus. Cancer Res 26:1787-1800.
Plessers, Bosmans, Cox, Raus (1986) Specific monoclonal antibodies reacting with human breast cancer cells. Anticancer Res 6:885-888.
Fridman, Kibbey, Royce, Zain, Sweeney, Jicha, Yanelli, Martin, Kleinman (1991) Enhanced tumor growth of both primary and established human and murine tumor cells in athymic mice after coinjection with matrigel. J Natl Cancer Inst 83:769-774.
Clarke, Brünner, Katzenellenbogen, Thompson, Norman, Koppi, Paik, Lippman, Dickson (1989) Progression of human breast cancer cells from hormone-dependent to hormone-independent growth both in vitro and in vivo. Proc Natl Acad Sci USA , 2nd English Edn.; 86:3649-3653.