ORBi: Baramova E. N. - Evaluation of Matrix Metalloproteinases and Serine Proteases Activities in Three B16 Melanoma Cell Lines with Distinct Tumorigenic Potential

Reference : Evaluation of Matrix Metalloproteinases and Serine Proteases Activities in Three B16 Mel...


	Document type :	Scientific journals : Article
	Discipline(s) :	Life sciences : Biochemistry, biophysics & molecular biology
	To cite this reference:	http://hdl.handle.net/2268/9935

Title :	Evaluation of Matrix Metalloproteinases and Serine Proteases Activities in Three B16 Melanoma Cell Lines with Distinct Tumorigenic Potential
Language :	English
Author, co-author :	Baramova, E. N. [> > > >]
	Coucke, P. [ > > ]
	Leprince, Pierre [Université de Liège - ULg > > CNCM/ Centre fac. de rech. en neurobiologie cell. et moléc. >]
	Gillet, Marie-Claire [Université de Liège - ULg > Département des sciences biomédicales et précliniques > Histologie - Cytologie - Département des sciences biomédicales et précliniques >]
	Bassleer, R. [ > > ]
	Foidart, Jean-Michel [Université de Liège - ULg > Département des sciences cliniques > Gynécologie - Obstétrique >]
Publication date :	1994
Journal title :	Anticancer Research
Publisher :	JG Delinassios Anticancer Research
Volume :	14
Issue/season :	3A, May-Jun
Pages :	841-6
Peer reviewed :	Yes (verified by ORBi)
ISSN :	0250-7005
City :	Athens
Country :	Greece
Abstract :	[en] Mouse B16 melanoma cells (B16, parental line) and two derived clones either pigmented (B16P) or non pigmented (B16NP) were cultured as monolayers (2D) or on agar, as aggregates (3D). The productions of gelatinases A and B (72 kDa and 92 kDa type IV collagenases) and their inhibitors (TIMP1 and TIMP2), plasminogen activators (PAs) and plasminogen activator inhibitors (PAI) were investigated. The B16 cell lines did not secrete any gelatinase, but they secreted TIMP2, tissue-type (t-PA), urokinase-type (u-PA) plasminogen activators and PAI-1 like activities. High levels of PAI activity were determined in conditioned media and cellular extracts of B16NP, which could account for the lower tumorigenic potential of these cells. In 3D cultures, the cellular extracts of the three cell lines contained essentially u-PA activity. This activity could contribute to the greater tumorigenic and invasive capacities of B16, B16P and B16NP when cultured in 3D.
Permalink :	http://hdl.handle.net/2268/9935

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