|Reference : BIOREMEDIATION DE SOLS POLLUES AUX HYDROCARBURES – AMELIORATION DE LA BIOREMEDIATION IN ...|
|Dissertations and theses : Master's dissertation|
|Life sciences : Biotechnology|
|BIOREMEDIATION DE SOLS POLLUES AUX HYDROCARBURES – AMELIORATION DE LA BIOREMEDIATION IN SITU DES HYDROCARBURES PAR BIO-AUGMENTATION AU MOYEN DE SOUCHES ENDOGENES ET EXOGENES|
|[en] Bioremediation of hydrocarbon polluted soil - Improvement of in situ bioremediation by bioaugmentation with endogenous and exogenous strains|
|Tarayre, Cédric [Université de Liège - ULg > > > Form. doct. sc. agro. & ingé. biol.]|
|Gembloux Agro-Bio Tech|
|Master en chimie et bio-industries|
|[en] bioremediation ; bioaugmentation ; hydrocarbures|
|[en] This study relates to biological treatment of hydrocarbon polluted soils. Its purpose is to establish an industrial approach of bioaugmentation and to verify its efficiency compared with biostimulation.
The purpose is to simulate in situ bioremediation using experimental columns. It has been demonstrated that it’s possible to produce a biomass able to degrade hydrocarbons in a polluted soil in one month. The main way of hydrocarbon elimination in the soil is biodegradation and the amount of hydrocarbons removed by water is negligible (1 % in each case). The addition of exogenous strains into the soil leads to the best biodegradation rate (39,3 % in 63 days). An endogenous bioaugmentation was tried but the efficiency was not demonstrated. On the other hand, the use of an artificial consortium was not more satisfying. Biostimulation alone led to a biodegradation rate of 23,9 % in 63 days. The lack of oxygen was avoided and evaluated through nitrite and ammonium concentrations in the water. These concentrations were negligible and always less than 2,5 and 1 mg/l, respectively. This work also tried to develop a special technique of monitoring of microbial ecology with microplates (Biolog ECO Micro PlateTM). The results were analysed through two different statistical methods : similarity rates and principal component analysis. It has been demonstrated that the column which leads to the best biodegradation rate also shows a particular metabolic profile, according to principal component analysis. The similarity rate is only 31 % with the initial microflora. This microflora seems to change during the experience because the similarity rate between the initial and the final microfloras is only 61 %.
This work proved the efficiency of exogenous bioaugmentation compared with biostimulation. It proposes to apply this technique on a large scale, but only if the amount of polluted soil is evaluated before.
|Gembloux Agro-Bio Tech, Unité de Bio-Industries|
|SANIFOX, Région Wallonne|
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