Reference : Development of an animal experimental model to study the effects of levonorgestrel on th...
Scientific journals : Article
Life sciences : Anatomy (cytology, histology, embryology...) & physiology
http://hdl.handle.net/2268/9931
Development of an animal experimental model to study the effects of levonorgestrel on the human endometrium.
English
Alvarez Gonzalez, Maria-Luz mailto [Université de Liège - ULg > Département des sciences cliniques > Labo de biologie des tumeurs et du développement >]
Galant, C. [> >]
Frankenne, F. [> >]
Nisolle, Michelle mailto [Centre Hospitalier Universitaire de Liège - CHU > > Gynécologie-Obstétrique CHR >]
Labied, Soraya mailto [Université de Liège - ULg > Département des sciences cliniques > Labo de biologie des tumeurs et du développement >]
Foidart, Jean-Michel mailto [Université de Liège - ULg > Département des sciences cliniques > Gynécologie - Obstétrique - Labo de biologie des tumeurs et du développement >]
Marbaix, E. [> >]
Beliard, Aude mailto [Centre Hospitalier Universitaire de Liège - CHU > > Gynécologie-Obstétrique CHR >]
2009
Human Reproduction
Oxford University Press
24
3
697-704
Yes (verified by ORBi)
International
0268-1161
1460-2350
Oxford
United Kingdom
[en] levonorgestrel ; endometrium ; mouse model ; endometrial transplants
[en] BACKGROUND: This study was designed to develop an animal model to test the response of endometrium to local progestin delivery.

METHODS: Proliferative human endometrium was subcutaneously grafted in two groups of SCID mice that received, 2 days before, a subcutaneous estradiol (E2) pellet and, for half of them, an additional implant of levonorgestrel (LNG). Mice were sacrificed 1, 2, 3 or 4 weeks after endometrial implantation and grafts were histologically analysed. Proliferation, steroid hormone receptors, blood vessels and stromal decidualization in both groups (E2 and LNG) were immunohistologically evaluated and compared with proliferative endometrium and endometrium from women with an LNG intrauterine device.

RESULTS: Grafts presented normal morphological endometrial characteristics. The expression of progesterone receptors was significantly decreased in glands and stroma of the LNG group as compared with the E2 group at all times. A significant decrease was also observed in the stromal expression of estrogen receptor- in the LNG group. At 4 weeks, the mean cross-sectional area of vessels was significantly higher after LNG treatment.

CONCLUSIONS: These morphological and immunohistochemical characteristics are similar to those observed in women treated with local LNG. This mouse model might facilitate further investigations needed to understand the mechanisms responsible for the breakthrough bleeding frequently observed in progestin users.
Giga-Cancer
http://hdl.handle.net/2268/9931

File(s) associated to this reference

Fulltext file(s):

FileCommentaryVersionSizeAccess
Open access
Alvarez Gonzalez ML 2009.pdf.pdfAuthor postprint590.83 kBView/Open

Bookmark and Share SFX Query

All documents in ORBi are protected by a user license.